Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1396830 | XP_013468976.1 | 92.222 | 90 | 7 | 0 | 1 | 90 | 1 | 90 | 1.80e-56 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1396830 | sp|P29062|PR4A_TOBAC | 65.060 | 83 | 27 | 1 | 8 | 90 | 11 | 91 | 5.94e-38 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1396830 | A0A072VNL8 | 92.222 | 90 | 7 | 0 | 1 | 90 | 1 | 90 | 8.62e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011720 | Msa1396830 | 0.809562 | 1.780092e-50 | -8.615850e-47 |
| Msa0012040 | Msa1396830 | 0.804440 | 2.157796e-49 | -8.615850e-47 |
| Msa0014800 | Msa1396830 | 0.830439 | 2.935165e-55 | -8.615850e-47 |
| Msa0032270 | Msa1396830 | 0.838355 | 3.020364e-57 | -8.615850e-47 |
| Msa0033340 | Msa1396830 | 0.830271 | 3.225771e-55 | -8.615850e-47 |
| Msa0051930 | Msa1396830 | 0.837361 | 5.439127e-57 | -8.615850e-47 |
| Msa0055730 | Msa1396830 | 0.809376 | 1.951143e-50 | -8.615850e-47 |
| Msa0061150 | Msa1396830 | 0.819581 | 1.077145e-52 | -8.615850e-47 |
| Msa0074080 | Msa1396830 | 0.806833 | 6.787834e-50 | -8.615850e-47 |
| Msa0074090 | Msa1396830 | 0.801106 | 1.052942e-48 | -8.615850e-47 |
| Msa0090100 | Msa1396830 | 0.806204 | 9.215445e-50 | -8.615850e-47 |
| Msa0092990 | Msa1396830 | 0.806335 | 8.647698e-50 | -8.615850e-47 |
| Msa0094920 | Msa1396830 | 0.807588 | 4.698203e-50 | -8.615850e-47 |
| Msa0100090 | Msa1396830 | 0.800440 | 1.439725e-48 | -8.615850e-47 |
| Msa0105700 | Msa1396830 | 0.839729 | 1.331131e-57 | -8.615850e-47 |
| Msa0107390 | Msa1396830 | 0.818246 | 2.166995e-52 | -8.615850e-47 |
| Msa0108560 | Msa1396830 | 0.851133 | 1.082400e-60 | -8.615850e-47 |
| Msa0115270 | Msa1396830 | 0.805517 | 1.285089e-49 | -8.615850e-47 |
| Msa1348950 | Msa1396830 | 0.822639 | 2.127528e-53 | -8.615850e-47 |
| Msa1358610 | Msa1396830 | -0.807925 | 3.983995e-50 | -8.615850e-47 |
| Msa1362880 | Msa1396830 | 0.837810 | 4.172787e-57 | -8.615850e-47 |
| Msa1369530 | Msa1396830 | 0.810806 | 9.595498e-51 | -8.615850e-47 |
| Msa1396830 | Msa1408790 | 0.813246 | 2.820466e-51 | -8.615850e-47 |
| Msa1396830 | Msa1411020 | 0.800154 | 1.646342e-48 | -8.615850e-47 |
| Msa1396830 | Msa1427230 | 0.803959 | 2.717001e-49 | -8.615850e-47 |
| Msa1396830 | Msa1427830 | 0.811413 | 7.090274e-51 | -8.615850e-47 |
| Msa1396830 | Msa1434350 | 0.800602 | 1.334248e-48 | -8.615850e-47 |
| Msa1396830 | Msa1436040 | 0.814057 | 1.870108e-51 | -8.615850e-47 |
| Msa1396830 | Msa1437720 | 0.801031 | 1.090681e-48 | -8.615850e-47 |
| Msa1396830 | Msa1438700 | 0.801733 | 7.832222e-49 | -8.615850e-47 |
| Msa1396830 | Msa1439320 | 0.803841 | 2.875313e-49 | -8.615850e-47 |
| Msa1396830 | Msa1441190 | 0.811238 | 7.738404e-51 | -8.615850e-47 |
| Msa1396830 | Msa1455490 | 0.806403 | 8.366384e-50 | -8.615850e-47 |
| Msa1396830 | Msa1460830 | 0.803151 | 3.997788e-49 | -8.615850e-47 |
| Msa1396830 | Msa1461250 | -0.803301 | 3.720635e-49 | -8.615850e-47 |
| Msa0607540 | Msa1396830 | 0.804335 | 2.269439e-49 | -8.615850e-47 |
| Msa0610370 | Msa1396830 | 0.815677 | 8.179328e-52 | -8.615850e-47 |
| Msa0621590 | Msa1396830 | 0.805239 | 1.469428e-49 | -8.615850e-47 |
| Msa0638280 | Msa1396830 | 0.834108 | 3.627354e-56 | -8.615850e-47 |
| Msa0638320 | Msa1396830 | 0.820042 | 8.454628e-53 | -8.615850e-47 |
| Msa0642180 | Msa1396830 | 0.814473 | 1.513531e-51 | -8.615850e-47 |
| Msa0647830 | Msa1396830 | 0.804376 | 2.225471e-49 | -8.615850e-47 |
| Msa0650900 | Msa1396830 | 0.811954 | 5.406161e-51 | -8.615850e-47 |
| Msa0653490 | Msa1396830 | 0.804535 | 2.061955e-49 | -8.615850e-47 |
| Msa0660300 | Msa1396830 | 0.827631 | 1.406098e-54 | -8.615850e-47 |
| Msa0662650 | Msa1396830 | 0.816844 | 4.484017e-52 | -8.615850e-47 |
| Msa0677270 | Msa1396830 | 0.811640 | 6.328428e-51 | -8.615850e-47 |
| Msa0689010 | Msa1396830 | 0.810035 | 1.407684e-50 | -8.615850e-47 |
| Msa0692110 | Msa1396830 | 0.804640 | 1.960050e-49 | -8.615850e-47 |
| Msa0693380 | Msa1396830 | 0.820049 | 8.423764e-53 | -8.615850e-47 |
| Msa0708860 | Msa1396830 | 0.810356 | 1.200329e-50 | -8.615850e-47 |
| Msa0718470 | Msa1396830 | 0.803829 | 2.891408e-49 | -8.615850e-47 |
| Msa0731180 | Msa1396830 | 0.835163 | 1.968745e-56 | -8.615850e-47 |
| Msa0734660 | Msa1396830 | 0.801102 | 1.054818e-48 | -8.615850e-47 |
| Msa0743260 | Msa1396830 | 0.808392 | 3.167345e-50 | -8.615850e-47 |
| Msa0771580 | Msa1396830 | 0.831283 | 1.822004e-55 | -8.615850e-47 |
| Msa0801100 | Msa1396830 | 0.801148 | 1.032082e-48 | -8.615850e-47 |
| Msa0152170 | Msa1396830 | 0.837601 | 4.721747e-57 | -8.615850e-47 |
| Msa0172410 | Msa1396830 | 0.853112 | 2.963952e-61 | -8.615850e-47 |
| Msa0172430 | Msa1396830 | 0.841461 | 4.684686e-58 | -8.615850e-47 |
| Msa0173990 | Msa1396830 | 0.805770 | 1.137218e-49 | -8.615850e-47 |
| Msa0191010 | Msa1396830 | 0.811579 | 6.522685e-51 | -8.615850e-47 |
| Msa0197660 | Msa1396830 | 0.811112 | 8.240262e-51 | -8.615850e-47 |
| Msa0199820 | Msa1396830 | 0.810715 | 1.004052e-50 | -8.615850e-47 |
| Msa0223380 | Msa1396830 | 0.814606 | 1.414088e-51 | -8.615850e-47 |
| Msa0225470 | Msa1396830 | 0.826808 | 2.213792e-54 | -8.615850e-47 |
| Msa0233300 | Msa1396830 | 0.819555 | 1.092184e-52 | -8.615850e-47 |
| Msa0233310 | Msa1396830 | 0.800539 | 1.374216e-48 | -8.615850e-47 |
| Msa0235190 | Msa1396830 | 0.801923 | 7.160419e-49 | -8.615850e-47 |
| Msa0236440 | Msa1396830 | 0.811289 | 7.541009e-51 | -8.615850e-47 |
| Msa1233500 | Msa1396830 | 0.840553 | 8.111255e-58 | -8.615850e-47 |
| Msa1233890 | Msa1396830 | 0.806237 | 9.069687e-50 | -8.615850e-47 |
| Msa1247250 | Msa1396830 | 0.808052 | 3.742709e-50 | -8.615850e-47 |
| Msa1257600 | Msa1396830 | 0.813354 | 2.670538e-51 | -8.615850e-47 |
| Msa1275360 | Msa1396830 | 0.800611 | 1.328875e-48 | -8.615850e-47 |
| Msa1275770 | Msa1396830 | 0.811314 | 7.449026e-51 | -8.615850e-47 |
| Msa1276440 | Msa1396830 | 0.804303 | 2.305069e-49 | -8.615850e-47 |
| Msa1288420 | Msa1396830 | 0.810131 | 1.342697e-50 | -8.615850e-47 |
| Msa1292730 | Msa1396830 | 0.804228 | 2.389103e-49 | -8.615850e-47 |
| Msa1297090 | Msa1396830 | 0.806382 | 8.451317e-50 | -8.615850e-47 |
| Msa1300350 | Msa1396830 | -0.804121 | 2.515317e-49 | -8.615850e-47 |
| Msa1303160 | Msa1396830 | 0.808879 | 2.493469e-50 | -8.615850e-47 |
| Msa1304840 | Msa1396830 | 0.834324 | 3.201773e-56 | -8.615850e-47 |
| Msa1306750 | Msa1396830 | 0.830591 | 2.694002e-55 | -8.615850e-47 |
| Msa1307740 | Msa1396830 | 0.827914 | 1.202208e-54 | -8.615850e-47 |
| Msa1308620 | Msa1396830 | 0.808947 | 2.410405e-50 | -8.615850e-47 |
| Msa1316560 | Msa1396830 | -0.800437 | 1.441651e-48 | -8.615850e-47 |
| Msa1333990 | Msa1396830 | 0.823976 | 1.036169e-53 | -8.615850e-47 |
| Msa0377530 | Msa1396830 | 0.815041 | 1.132649e-51 | -8.615850e-47 |
| Msa0380040 | Msa1396830 | 0.826638 | 2.430582e-54 | -8.615850e-47 |
| Msa0389810 | Msa1396830 | 0.833749 | 4.459061e-56 | -8.615850e-47 |
| Msa0394340 | Msa1396830 | 0.800879 | 1.171834e-48 | -8.615850e-47 |
| Msa0404030 | Msa1396830 | 0.822886 | 1.863805e-53 | -8.615850e-47 |
| Msa0419970 | Msa1396830 | 0.802981 | 4.334984e-49 | -8.615850e-47 |
| Msa0429290 | Msa1396830 | 0.819951 | 8.869299e-53 | -8.615850e-47 |
| Msa0442850 | Msa1396830 | 0.815879 | 7.374290e-52 | -8.615850e-47 |
| Msa0445280 | Msa1396830 | 0.805418 | 1.347683e-49 | -8.615850e-47 |
| Msa0449760 | Msa1396830 | 0.801606 | 8.319108e-49 | -8.615850e-47 |
| Msa0449830 | Msa1396830 | 0.824580 | 7.475191e-54 | -8.615850e-47 |
| Msa0466280 | Msa1396830 | 0.803365 | 3.609687e-49 | -8.615850e-47 |
| Msa0468130 | Msa1396830 | 0.824976 | 6.026358e-54 | -8.615850e-47 |
| Msa0473240 | Msa1396830 | 0.840453 | 8.616080e-58 | -8.615850e-47 |
| Msa0475410 | Msa1396830 | 0.801795 | 7.607853e-49 | -8.615850e-47 |
| Msa0475580 | Msa1396830 | 0.809939 | 1.476713e-50 | -8.615850e-47 |
| Msa0982270 | Msa1396830 | 0.833242 | 5.969129e-56 | -8.615850e-47 |
| Msa0991670 | Msa1396830 | 0.809498 | 1.836452e-50 | -8.615850e-47 |
| Msa0992490 | Msa1396830 | -0.821792 | 3.343677e-53 | -8.615850e-47 |
| Msa0999040 | Msa1396830 | 0.800584 | 1.345624e-48 | -8.615850e-47 |
| Msa1021340 | Msa1396830 | 0.806545 | 7.807848e-50 | -8.615850e-47 |
| Msa1026760 | Msa1396830 | 0.827127 | 1.857004e-54 | -8.615850e-47 |
| Msa1026790 | Msa1396830 | 0.830677 | 2.566952e-55 | -8.615850e-47 |
| Msa1027290 | Msa1396830 | 0.855224 | 7.281029e-62 | -8.615850e-47 |
| Msa1039880 | Msa1396830 | 0.811203 | 7.873018e-51 | -8.615850e-47 |
| Msa1043050 | Msa1396830 | 0.802376 | 5.777136e-49 | -8.615850e-47 |
| Msa1053260 | Msa1396830 | 0.817363 | 3.428816e-52 | -8.615850e-47 |
| Msa1059420 | Msa1396830 | 0.808691 | 2.735033e-50 | -8.615850e-47 |
| Msa1077950 | Msa1396830 | 0.821906 | 3.147955e-53 | -8.615850e-47 |
| Msa1082500 | Msa1396830 | 0.825532 | 4.453015e-54 | -8.615850e-47 |
| Msa1085920 | Msa1396830 | 0.800571 | 1.354020e-48 | -8.615850e-47 |
| Msa1087470 | Msa1396830 | 0.812865 | 3.419003e-51 | -8.615850e-47 |
| Msa1095480 | Msa1396830 | 0.820858 | 5.493657e-53 | -8.615850e-47 |
| Msa0239800 | Msa1396830 | 0.810155 | 1.326561e-50 | -8.615850e-47 |
| Msa0258760 | Msa1396830 | 0.838515 | 2.746944e-57 | -8.615850e-47 |
| Msa0260210 | Msa1396830 | 0.810344 | 1.207981e-50 | -8.615850e-47 |
| Msa0269450 | Msa1396830 | 0.808492 | 3.015290e-50 | -8.615850e-47 |
| Msa0280570 | Msa1396830 | 0.824534 | 7.660316e-54 | -8.615850e-47 |
| Msa0304290 | Msa1396830 | 0.812809 | 3.516283e-51 | -8.615850e-47 |
| Msa0304330 | Msa1396830 | 0.812019 | 5.232940e-51 | -8.615850e-47 |
| Msa0305890 | Msa1396830 | 0.815844 | 7.508380e-52 | -8.615850e-47 |
| Msa0309550 | Msa1396830 | 0.801455 | 8.931696e-49 | -8.615850e-47 |
| Msa0311640 | Msa1396830 | 0.814837 | 1.256811e-51 | -8.615850e-47 |
| Msa0311660 | Msa1396830 | 0.825573 | 4.353830e-54 | -8.615850e-47 |
| Msa0313780 | Msa1396830 | 0.805137 | 1.543546e-49 | -8.615850e-47 |
| Msa0313920 | Msa1396830 | 0.833667 | 4.675395e-56 | -8.615850e-47 |
| Msa0317510 | Msa1396830 | 0.816127 | 6.489886e-52 | -8.615850e-47 |
| Msa0323080 | Msa1396830 | 0.812861 | 3.425102e-51 | -8.615850e-47 |
| Msa0326670 | Msa1396830 | 0.809622 | 1.727302e-50 | -8.615850e-47 |
| Msa0329700 | Msa1396830 | 0.823798 | 1.140694e-53 | -8.615850e-47 |
| Msa0339620 | Msa1396830 | 0.801216 | 9.996832e-49 | -8.615850e-47 |
| Msa0340810 | Msa1396830 | 0.815191 | 1.049229e-51 | -8.615850e-47 |
| Msa0344830 | Msa1396830 | 0.827222 | 1.762878e-54 | -8.615850e-47 |
| Msa0347360 | Msa1396830 | 0.832860 | 7.427173e-56 | -8.615850e-47 |
| Msa0350670 | Msa1396830 | 0.814816 | 1.270427e-51 | -8.615850e-47 |
| Msa0350990 | Msa1396830 | 0.815586 | 8.570576e-52 | -8.615850e-47 |
| Msa0351030 | Msa1396830 | 0.812715 | 3.688327e-51 | -8.615850e-47 |
| Msa0356960 | Msa1396830 | 0.805124 | 1.553068e-49 | -8.615850e-47 |
| Msa1107800 | Msa1396830 | 0.804102 | 2.537276e-49 | -8.615850e-47 |
| Msa1111700 | Msa1396830 | 0.817544 | 3.121670e-52 | -8.615850e-47 |
| Msa1116690 | Msa1396830 | 0.815469 | 9.099656e-52 | -8.615850e-47 |
| Msa1142500 | Msa1396830 | 0.839215 | 1.810865e-57 | -8.615850e-47 |
| Msa1151710 | Msa1396830 | 0.834191 | 3.457070e-56 | -8.615850e-47 |
| Msa1154160 | Msa1396830 | 0.801721 | 7.878890e-49 | -8.615850e-47 |
| Msa1198090 | Msa1396830 | 0.811751 | 5.986564e-51 | -8.615850e-47 |
| Msa1206020 | Msa1396830 | 0.808062 | 3.723821e-50 | -8.615850e-47 |
| Msa1207380 | Msa1396830 | 0.831494 | 1.617008e-55 | -8.615850e-47 |
| Msa1207420 | Msa1396830 | 0.804209 | 2.410748e-49 | -8.615850e-47 |
| Msa0484780 | Msa1396830 | 0.838651 | 2.534060e-57 | -8.615850e-47 |
| Msa0489610 | Msa1396830 | 0.805651 | 1.204508e-49 | -8.615850e-47 |
| Msa0500720 | Msa1396830 | 0.803968 | 2.705470e-49 | -8.615850e-47 |
| Msa0517330 | Msa1396830 | 0.805722 | 1.163809e-49 | -8.615850e-47 |
| Msa0517490 | Msa1396830 | 0.816518 | 5.307140e-52 | -8.615850e-47 |
| Msa0519220 | Msa1396830 | 0.805764 | 1.140364e-49 | -8.615850e-47 |
| Msa0535060 | Msa1396830 | 0.802056 | 6.723568e-49 | -8.615850e-47 |
| Msa0540070 | Msa1396830 | 0.829536 | 4.872778e-55 | -8.615850e-47 |
| Msa0553460 | Msa1396830 | 0.812253 | 4.652689e-51 | -8.615850e-47 |
| Msa0555950 | Msa1396830 | 0.813418 | 2.586162e-51 | -8.615850e-47 |
| Msa0558690 | Msa1396830 | 0.802935 | 4.430517e-49 | -8.615850e-47 |
| Msa0562440 | Msa1396830 | 0.827530 | 1.487087e-54 | -8.615850e-47 |
| Msa0585900 | Msa1396830 | 0.807495 | 4.915987e-50 | -8.615850e-47 |
| Msa0587690 | Msa1396830 | 0.819732 | 9.951252e-53 | -8.615850e-47 |
| Msa0855310 | Msa1396830 | 0.800461 | 1.425513e-48 | -8.615850e-47 |
| Msa0870620 | Msa1396830 | 0.805653 | 1.203224e-49 | -8.615850e-47 |
| Msa0874500 | Msa1396830 | 0.830447 | 2.921560e-55 | -8.615850e-47 |
| Msa0877410 | Msa1396830 | 0.800679 | 1.287126e-48 | -8.615850e-47 |
| Msa0898370 | Msa1396830 | 0.800686 | 1.282622e-48 | -8.615850e-47 |
| Msa0905720 | Msa1396830 | 0.812682 | 3.749065e-51 | -8.615850e-47 |
| Msa0907070 | Msa1396830 | 0.805504 | 1.293080e-49 | -8.615850e-47 |
| Msa0908170 | Msa1396830 | 0.809364 | 1.962941e-50 | -8.615850e-47 |
| Msa0910980 | Msa1396830 | -0.814576 | 1.435915e-51 | -8.615850e-47 |
| Msa0927670 | Msa1396830 | 0.821879 | 3.193505e-53 | -8.615850e-47 |
| Msa0960160 | Msa1396830 | 0.811122 | 8.200234e-51 | -8.615850e-47 |
| Msa0974630 | Msa1396830 | 0.807067 | 6.058267e-50 | -8.615850e-47 |
| Msa0976250 | Msa1396830 | 0.812428 | 4.261421e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1396830 | MtrunA17_Chr1g0190671 | 92.222 | 90 | 7 | 0 | 1 | 90 | 1 | 90 | 1.66e-60 | 181 |
| Msa1396830 | MtrunA17_Chr1g0190661 | 87.778 | 90 | 11 | 0 | 1 | 90 | 1 | 90 | 1.52e-58 | 176 |
| Msa1396830 | MtrunA17_Chr1g0190651 | 86.517 | 89 | 12 | 0 | 1 | 89 | 1 | 89 | 1.45e-55 | 168 |
| Msa1396830 | MtrunA17_Chr1g0190641 | 61.728 | 81 | 27 | 2 | 10 | 90 | 9 | 85 | 5.94e-32 | 108 |
| Msa1396830 | MtrunA17_Chr7g0274181 | 70.968 | 62 | 18 | 0 | 29 | 90 | 69 | 130 | 6.76e-29 | 102 |
| Msa1396830 | MtrunA17_Chr6g0484021 | 53.488 | 86 | 31 | 3 | 10 | 89 | 31 | 113 | 7.99e-25 | 96.3 |
| Msa1396830 | MtrunA17_Chr6g0483971 | 51.250 | 80 | 34 | 2 | 10 | 89 | 11 | 85 | 5.98e-23 | 89.4 |
| Msa1396830 | MtrunA17_Chr6g0484031 | 47.500 | 80 | 35 | 2 | 10 | 89 | 11 | 83 | 3.98e-19 | 80.1 |
| Msa1396830 | MtrunA17_Chr6g0484041 | 47.674 | 86 | 34 | 4 | 10 | 89 | 23 | 103 | 3.46e-17 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1396830 | AT3G04720.1 | 70.149 | 67 | 20 | 0 | 24 | 90 | 71 | 137 | 6.61e-32 | 110 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAACCCTCAGAATATTAAC+TGG | 0.317116 | tig0022086:+85126 | Msa1396830:CDS |
| ACATAGAAGTTTAAGCTTGA+TGG | 0.365054 | tig0022086:+85028 | Msa1396830:CDS |
| TTGTCATGGCGTCAAAAGTA+TGG | 0.371251 | tig0022086:+85200 | Msa1396830:CDS |
| GTCTCTACCGCGAGGACCCT+GGG | 0.394792 | tig0022086:-85243 | None:intergenic |
| CTCTTGCGGCAAGTGCTTGA+GGG | 0.475168 | tig0022086:+85265 | Msa1396830:CDS |
| AGGACCCTGGGGTCCACAAA+AGG | 0.477350 | tig0022086:-85231 | None:intergenic |
| AGTCTCTACCGCGAGGACCC+TGG | 0.482201 | tig0022086:-85244 | None:intergenic |
| GTCAGAGTGCAAACAACGTA+AGG | 0.487660 | tig0022086:+85087 | Msa1396830:CDS |
| ACTCTTGCGGCAAGTGCTTG+AGG | 0.504119 | tig0022086:+85264 | Msa1396830:CDS |
| TAATATTCTGAGGGTTGTAA+AGG | 0.506256 | tig0022086:-85121 | None:intergenic |
| TATTCTGAGGGTTGTAAAGG+TGG | 0.518272 | tig0022086:-85118 | None:intergenic |
| CCAGTGTATACTGTGCTACA+TGG | 0.518274 | tig0022086:+85162 | Msa1396830:CDS |
| CATGGCGTCAAAAGTATGGC+TGG | 0.519495 | tig0022086:+85204 | Msa1396830:CDS |
| TAGTCCCAGTTAATATTCTG+AGG | 0.529315 | tig0022086:-85131 | None:intergenic |
| TTTGACGCCATGACAAGGGC+TGG | 0.551508 | tig0022086:-85193 | None:intergenic |
| CAACCCTCAGAATATTAACT+GGG | 0.554793 | tig0022086:+85127 | Msa1396830:CDS |
| ATGCCAACCAGCCCTTGTCA+TGG | 0.561952 | tig0022086:+85186 | Msa1396830:CDS |
| ACGCCATGACAAGGGCTGGT+TGG | 0.589508 | tig0022086:-85189 | None:intergenic |
| AGTCCCAGTTAATATTCTGA+GGG | 0.592868 | tig0022086:-85130 | None:intergenic |
| CAGTGTATACTGTGCTACAT+GGG | 0.615919 | tig0022086:+85163 | Msa1396830:CDS |
| TCTCTACCGCGAGGACCCTG+GGG | 0.650500 | tig0022086:-85242 | None:intergenic |
| GTGGGAACGATGTTAGTGAA+TGG | 0.656794 | tig0022086:+85065 | Msa1396830:CDS |
| TCAGAGTGCAAACAACGTAA+GGG | 0.659863 | tig0022086:+85088 | Msa1396830:CDS |
| CCATGTAGCACAGTATACAC+TGG | 0.663920 | tig0022086:-85162 | None:intergenic |
| TCACTAACATCGTTCCCACA+AGG | 0.670804 | tig0022086:-85061 | None:intergenic |
| TGTGGACCCCAGGGTCCTCG+CGG | 0.678697 | tig0022086:+85236 | Msa1396830:CDS |
| CCGCAAGAGTCTCTACCGCG+AGG | 0.740415 | tig0022086:-85251 | None:intergenic |
| CCTCGCGGTAGAGACTCTTG+CGG | 0.755069 | tig0022086:+85251 | Msa1396830:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATAGAAGTTTAAGCTTGA+TGG | + | tig0022086:85028-85047 | Msa1396830:CDS | 30.0% | |
| ! | TAATATTCTGAGGGTTGTAA+AGG | - | tig0022086:85124-85143 | None:intergenic | 30.0% |
| !!! | ATGGTGTTTTGTTTCCTTGT+GGG | + | tig0022086:85047-85066 | Msa1396830:CDS | 35.0% |
| ACAACCCTCAGAATATTAAC+TGG | + | tig0022086:85126-85145 | Msa1396830:CDS | 35.0% | |
| CAACCCTCAGAATATTAACT+GGG | + | tig0022086:85127-85146 | Msa1396830:CDS | 35.0% | |
| AGTCCCAGTTAATATTCTGA+GGG | - | tig0022086:85133-85152 | None:intergenic | 35.0% | |
| TAGTCCCAGTTAATATTCTG+AGG | - | tig0022086:85134-85153 | None:intergenic | 35.0% | |
| !!! | GATGGTGTTTTGTTTCCTTG+TGG | + | tig0022086:85046-85065 | Msa1396830:CDS | 40.0% |
| TCAGAGTGCAAACAACGTAA+GGG | + | tig0022086:85088-85107 | Msa1396830:CDS | 40.0% | |
| ! | TATTCTGAGGGTTGTAAAGG+TGG | - | tig0022086:85121-85140 | None:intergenic | 40.0% |
| CAGTGTATACTGTGCTACAT+GGG | + | tig0022086:85163-85182 | Msa1396830:CDS | 40.0% | |
| ! | TACTTTTGACGCCATGACAA+GGG | - | tig0022086:85200-85219 | None:intergenic | 40.0% |
| ! | ATACTTTTGACGCCATGACA+AGG | - | tig0022086:85201-85220 | None:intergenic | 40.0% |
| TTGTCATGGCGTCAAAAGTA+TGG | + | tig0022086:85200-85219 | Msa1396830:CDS | 40.0% | |
| TCACTAACATCGTTCCCACA+AGG | - | tig0022086:85064-85083 | None:intergenic | 45.0% | |
| GTGGGAACGATGTTAGTGAA+TGG | + | tig0022086:85065-85084 | Msa1396830:CDS | 45.0% | |
| GTCAGAGTGCAAACAACGTA+AGG | + | tig0022086:85087-85106 | Msa1396830:CDS | 45.0% | |
| CCATGTAGCACAGTATACAC+TGG | - | tig0022086:85165-85184 | None:intergenic | 45.0% | |
| CCAGTGTATACTGTGCTACA+TGG | + | tig0022086:85162-85181 | Msa1396830:CDS | 45.0% | |
| ! | CATGGCGTCAAAAGTATGGC+TGG | + | tig0022086:85204-85223 | Msa1396830:CDS | 50.0% |
| ! | TATGGCTGGACTGCCTTTTG+TGG | + | tig0022086:85218-85237 | Msa1396830:CDS | 50.0% |
| ATGCCAACCAGCCCTTGTCA+TGG | + | tig0022086:85186-85205 | Msa1396830:CDS | 55.0% | |
| TTTGACGCCATGACAAGGGC+TGG | - | tig0022086:85196-85215 | None:intergenic | 55.0% | |
| ! | ACTGCCTTTTGTGGACCCCA+GGG | + | tig0022086:85227-85246 | Msa1396830:CDS | 55.0% |
| ! | ACGCCATGACAAGGGCTGGT+TGG | - | tig0022086:85192-85211 | None:intergenic | 60.0% |
| ! | GACTGCCTTTTGTGGACCCC+AGG | + | tig0022086:85226-85245 | Msa1396830:CDS | 60.0% |
| AGGACCCTGGGGTCCACAAA+AGG | - | tig0022086:85234-85253 | None:intergenic | 60.0% | |
| CCTCGCGGTAGAGACTCTTG+CGG | + | tig0022086:85251-85270 | Msa1396830:CDS | 60.0% | |
| TCTCTACCGCGAGGACCCTG+GGG | - | tig0022086:85245-85264 | None:intergenic | 65.0% | |
| GTCTCTACCGCGAGGACCCT+GGG | - | tig0022086:85246-85265 | None:intergenic | 65.0% | |
| AGTCTCTACCGCGAGGACCC+TGG | - | tig0022086:85247-85266 | None:intergenic | 65.0% | |
| CCGCAAGAGTCTCTACCGCG+AGG | - | tig0022086:85254-85273 | None:intergenic | 65.0% | |
| TGTGGACCCCAGGGTCCTCG+CGG | + | tig0022086:85236-85255 | Msa1396830:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022086 | gene | 85017 | 85286 | 85017 | ID=Msa1396830;Name=Msa1396830 |
| tig0022086 | mRNA | 85017 | 85286 | 85017 | ID=Msa1396830-mRNA-1;Parent=Msa1396830;Name=Msa1396830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|90 |
| tig0022086 | exon | 85017 | 85286 | 85017 | ID=Msa1396830-mRNA-1:exon:15704;Parent=Msa1396830-mRNA-1 |
| tig0022086 | CDS | 85017 | 85286 | 85017 | ID=Msa1396830-mRNA-1:cds;Parent=Msa1396830-mRNA-1 |
| Gene Sequence |
| Protein sequence |
