AlfalfaGEDB | Msa0413870

M. sativa cultivar zhongmu-4 / Msa0413870

KEGG: K13423|K13424
GO: GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009700 GO:0009889 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010035 GO:0010120 GO:0010200 GO:0010243 GO:0010286 GO:0010468 GO:0010506 GO:0010508 GO:0010556 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0031323 GO:0031325 GO:0031326 GO:0031329 GO:0031331 GO:0033554 GO:0034605 GO:0034641 GO:0042221 GO:0042430 GO:0042435 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0070370 GO:0071704 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413870	AKM20827.1	97.852	512	9	2	1	511	1	511	0.0	1026

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413870	sp\|Q6B6R4\|WRK24_ORYSI	56.171	397	131	11	62	427	65	449	1.20e-131	396

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413870	A0A0G3Y2B1	97.852	512	9	2	1	511	1	511	0.0	1026

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TFs/TRs:

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Gene ID	Type	Classification
Msa0413870	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001830	Msa0413870	0.804479	2.118057e-49	-8.615850e-47
Msa0004480	Msa0413870	0.825105	5.618958e-54	-8.615850e-47
Msa0006060	Msa0413870	0.839731	1.329568e-57	-8.615850e-47
Msa0090380	Msa0413870	0.828751	7.551753e-55	-8.615850e-47
Msa0137860	Msa0413870	0.830252	3.260145e-55	-8.615850e-47
Msa0138900	Msa0413870	0.812972	3.238787e-51	-8.615850e-47
Msa0187310	Msa0413870	0.811285	7.559085e-51	-8.615850e-47
Msa0196880	Msa0413870	0.885249	9.571182e-72	-8.615850e-47
Msa0223140	Msa0413870	0.833870	4.158939e-56	-8.615850e-47
Msa0224130	Msa0413870	0.819842	9.389347e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413870	MtrunA17_Chr3g0106491	92.982	513	29	2	1	511	1	508	0.0	918
Msa0413870	MtrunA17_Chr5g0432161	56.966	567	167	18	1	510	35	581	1.13e-163	476
Msa0413870	MtrunA17_Chr7g0243891	47.818	527	200	21	39	511	37	542	1.04e-125	378
Msa0413870	MtrunA17_Chr4g0004911	46.089	473	202	14	61	510	47	489	3.25e-106	326
Msa0413870	MtrunA17_Chr5g0445311	44.313	422	180	11	40	427	28	428	3.38e-94	296
Msa0413870	MtrunA17_Chr5g0445311	49.351	77	36	2	363	439	200	273	8.90e-14	73.9
Msa0413870	MtrunA17_Chr3g0101131	43.439	442	183	13	40	425	47	477	5.55e-92	292
Msa0413870	MtrunA17_Chr3g0101131	40.650	123	63	4	183	300	406	523	5.66e-17	84.0
Msa0413870	MtrunA17_Chr5g0418191	50.719	278	128	5	185	457	140	413	2.64e-82	262
Msa0413870	MtrunA17_Chr7g0218081	40.144	416	192	10	41	431	95	478	1.36e-80	260

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0413870	AT2G38470.1	43.548	558	227	19	1	510	1	518	5.95e-111	339
Msa0413870	AT5G07100.1	48.087	366	91	10	62	425	28	296	1.73e-93	287
Msa0413870	AT2G03340.1	45.226	398	187	9	39	429	108	481	5.18e-93	293
Msa0413870	AT2G03340.1	60.000	70	27	1	363	432	250	318	1.41e-19	92.0
Msa0413870	AT4G26640.2	43.409	440	176	12	40	432	37	450	6.72e-91	288
Msa0413870	AT4G26640.7	43.953	430	173	12	51	432	1	410	1.66e-90	286
Msa0413870	AT2G30250.1	45.000	440	173	15	1	423	1	388	3.14e-89	279
Msa0413870	AT2G30250.1	55.385	65	28	1	197	260	329	393	8.06e-16	79.7
Msa0413870	AT4G26640.5	56.911	246	93	4	195	432	34	274	5.85e-87	273
Msa0413870	AT4G26640.6	56.911	246	93	4	195	432	34	274	5.85e-87	273

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Find 120 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACATTCCTTCAAGAT+AGG	0.411668	3_2:+58786271	None:intergenic
AAAGTTGAGAGGGGTTTAGA+TGG	0.393749	3_2:-58788420	Msa0413870:CDS
AAATCAATAAATTGAAGGGT+TGG	0.439082	3_2:+58789921	None:intergenic
AAATGAAATTCAAATGGATT+TGG	0.360393	3_2:-58788268	Msa0413870:CDS
AACAATGTTTCACTTCATAA+TGG	0.317177	3_2:-58786435	Msa0413870:CDS
AAGAGAGGTGTAGGAGAAAT+AGG	0.219713	3_2:+58789678	None:intergenic
AAGGAGGTCAGAGAAGAAAG+AGG	0.508130	3_2:+58789818	None:intergenic
AAGTGTTGTCGGATCAAAAC+AGG	0.453220	3_2:-58789746	Msa0413870:CDS
AATGAGATGATGCAGAACAA+TGG	0.599597	3_2:-58786402	Msa0413870:CDS
AATGATCCGGTAGTTGGAGA+TGG	0.563554	3_2:+58789113	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGTATAGAAAAGTAAGAAGA+AGG	+	chr3_2:58786623-58786642	None:intergenic	30.0%
TATGTCTATGTGATTCTAGT+TGG	-	chr3_2:58787092-58787111	Msa0413870:intron	30.0%
CTAATAATGAAGGCTTCAAT+TGG	-	chr3_2:58787183-58787202	Msa0413870:intron	30.0%
AAACTTTCATGTATACCAAC+AGG	-	chr3_2:58787568-58787587	Msa0413870:intron	30.0%
AACTTTCATGTATACCAACA+GGG	-	chr3_2:58787569-58787588	Msa0413870:intron	30.0%
GGATACAATTGGAGAAAATA+TGG	-	chr3_2:58787752-58787771	Msa0413870:intron	30.0%
TATGGACAAAAACAAGTGAA+AGG	-	chr3_2:58787770-58787789	Msa0413870:CDS	30.0%
CAACAAAGAAAAAAGTTGAG+AGG	-	chr3_2:58787840-58787859	Msa0413870:CDS	30.0%
ACAAAGAAAAAAGTTGAGAG+GGG	-	chr3_2:58787842-58787861	Msa0413870:CDS	30.0%
TTTCCAAAATGATTTGAAGG+AGG	+	chr3_2:58787986-58788005	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	58786284	58790009	58786284	ID=Msa0413870;Name=Msa0413870
chr3_2	mRNA	58786284	58790009	58786284	ID=Msa0413870-mRNA-1;Parent=Msa0413870;Name=Msa0413870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=162\|1\|0.8\|1\|1\|1\|5\|0\|511
chr3_2	exon	58786284	58786671	58786284	ID=Msa0413870-mRNA-1:exon:12189;Parent=Msa0413870-mRNA-1
chr3_2	exon	58787765	58787929	58787765	ID=Msa0413870-mRNA-1:exon:12188;Parent=Msa0413870-mRNA-1
chr3_2	exon	58788150	58788703	58788150	ID=Msa0413870-mRNA-1:exon:12187;Parent=Msa0413870-mRNA-1
chr3_2	exon	58788968	58789141	58788968	ID=Msa0413870-mRNA-1:exon:12186;Parent=Msa0413870-mRNA-1
chr3_2	exon	58789593	58790009	58789593	ID=Msa0413870-mRNA-1:exon:12185;Parent=Msa0413870-mRNA-1
chr3_2	five_prime_UTR	58789848	58790009	58789848	ID=Msa0413870-mRNA-1:five_prime_utr;Parent=Msa0413870-mRNA-1
chr3_2	CDS	58789593	58789847	58789593	ID=Msa0413870-mRNA-1:cds;Parent=Msa0413870-mRNA-1
chr3_2	CDS	58788968	58789141	58788968	ID=Msa0413870-mRNA-1:cds;Parent=Msa0413870-mRNA-1
chr3_2	CDS	58788150	58788703	58788150	ID=Msa0413870-mRNA-1:cds;Parent=Msa0413870-mRNA-1
chr3_2	CDS	58787765	58787929	58787765	ID=Msa0413870-mRNA-1:cds;Parent=Msa0413870-mRNA-1
chr3_2	CDS	58786284	58786671	58786284	ID=Msa0413870-mRNA-1:cds;Parent=Msa0413870-mRNA-1

Gene Sequence

>Msa0413870

ATGACCTCCTCTTTCTTCTCTGACCTCCTTAATTCTCCAACCGACCAAAACCTTAAAGACACCGAAAGATCTCATCATCAAGTGTTGTCGGATCAAAACAGGTCGGGTTTACCCCCCAAATTCAAGTCTACACCTCCTCCATCTCTTCCTATTTCTCCTACACCTCTCTTTTCTCCTTCTTCTTACTTTTCTATACCTCCTGGCTTTTCTCTTTCTGAGCTTCTTGACTCTCCTGTTCTCCTTAACTCTTCTCATATTCTTCCATCTCCAACTACCGGATCATTTGCTAATAATGAAGGCTTCAATTGGAAGAATAATTATGAGGAAAAGCAAGAGAATGTCAAGGCAGAAGAGGAAACCATCTCATCAAGTTTCTCCTTCCAAGCTCAACCAGCTGGGTTTCAATCTTCGACCACCACGGTTCAAAATGGATGGAACATGGTGAAAACTGAAAACTCTTCTTCAATGCAAAGTTTCCCACCAGAGAGTAATTCTACTAGTGAACAAAACAACAAGAGTGGTTCACAATCAAATTACAACAATTATCAATCACAGCCACAAGTTCAAATCCTTAGTAGAAGATCAGATGATGGATACAATTGGAGAAAATATGGACAAAAACAAGTGAAAGGAAGTGAAAATCCAAGAAGCTATTACAAATGCACATACCCTAATTGTCCAACAAAGAAAAAAGTTGAGAGGGGTTTAGATGGACAAATTACTGAGATTGTTTACAAAGGTAGTCATAATCATCCTAAGCCTGTGGCTAACAAGAGGAACACAAACTCGTTGTCTTCCTCGTCGTCGTCACTGGCAAATCCTCCTCCTTCAAATCATTTTGGAAATGAAATTCAAATGGATTTGGTTGCTACACCGGAGAATTCATCGATATCGATTGGTGATGATGAATTTGAGCAGACTTCTCATAAATCTGGTGGAGATCATGATCAGTTTTGTGAAGATGAACCTGATTCCAAAAAATGGAGAATAGAAGGTGAAAATGAGGGAATATCATTAGGAGCTGGAAATAGAACAGTGAGGGAACCAAGAGTTGTTGTTCAAACAACAAGTGACATTGATATTCTAGATGATGGATACAGATGGAGGAAATATGGGCAGAAAGTAGTAAAGGGAAATCCAAATCCAAGGAGTTACTACAAGTGCACAAACCCAGGATGTCCAGTGAGAAAACATGTCGAACGAGCCTCACAAGACCTAAGAGCAGTGATAACAACGTATGAAGGAAAACACAATCATGATGTTCCTGCACCACGTGGAAGTGGAAACCACTCTATCAACAAACCACTACCAATGCAAACAACTATGAACAATACCACAAGTGCCAACAATTCCATCAACAACAACAATGTTTCACTTCATAATGGATTCTTCAACAATGAGATGATGCAGAACAATGGATTCACGGTTGATTCTTACATGAATATGAATCAACAACGTGTTAATAATATTATTAGAGGTAGAGCCAAGGAGGAGCCTGTAGAAGATGACTCATTTTTTGAATCTTTCCTATCTTGA

Protein sequence

>Msa0413870

MTSSFFSDLLNSPTDQNLKDTERSHHQVLSDQNRSGLPPKFKSTPPPSLPISPTPLFSPSSYFSIPPGFSLSELLDSPVLLNSSHILPSPTTGSFANNEGFNWKNNYEEKQENVKAEEETISSSFSFQAQPAGFQSSTTTVQNGWNMVKTENSSSMQSFPPESNSTSEQNNKSGSQSNYNNYQSQPQVQILSRRSDDGYNWRKYGQKQVKGSENPRSYYKCTYPNCPTKKKVERGLDGQITEIVYKGSHNHPKPVANKRNTNSLSSSSSSLANPPPSNHFGNEIQMDLVATPENSSISIGDDEFEQTSHKSGGDHDQFCEDEPDSKKWRIEGENEGISLGAGNRTVREPRVVVQTTSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTNPGCPVRKHVERASQDLRAVITTYEGKHNHDVPAPRGSGNHSINKPLPMQTTMNNTTSANNSINNNNVSLHNGFFNNEMMQNNGFTVDSYMNMNQQRVNNIIRGRAKEEPVEDDSFFESFLS*