Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1059670 | XP_013448654.2 | 95.773 | 828 | 34 | 1 | 1 | 828 | 11 | 837 | 0.0 | 1644 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1059670 | sp|Q9SXB8|Y1133_ARATH | 49.077 | 813 | 386 | 13 | 35 | 828 | 39 | 842 | 0.0 | 778 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1059670 | A0A396GZH2 | 95.773 | 828 | 34 | 1 | 1 | 828 | 11 | 837 | 0.0 | 1644 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001830 | Msa1059670 | 0.803274 | 3.770125e-49 | -8.615850e-47 |
| Msa0006060 | Msa1059670 | 0.835388 | 1.727757e-56 | -8.615850e-47 |
| Msa0026630 | Msa1059670 | 0.845125 | 4.936011e-59 | -8.615850e-47 |
| Msa0041840 | Msa1059670 | 0.826480 | 2.651263e-54 | -8.615850e-47 |
| Msa0051960 | Msa1059670 | 0.834365 | 3.126801e-56 | -8.615850e-47 |
| Msa0059900 | Msa1059670 | 0.807663 | 4.527719e-50 | -8.615850e-47 |
| Msa0061220 | Msa1059670 | 0.803120 | 4.055936e-49 | -8.615850e-47 |
| Msa0090380 | Msa1059670 | 0.852191 | 5.427746e-61 | -8.615850e-47 |
| Msa0094710 | Msa1059670 | 0.827372 | 1.622331e-54 | -8.615850e-47 |
| Msa0101960 | Msa1059670 | 0.835539 | 1.582111e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1059670 | MtrunA17_Chr7g0236231 | 95.773 | 828 | 34 | 1 | 1 | 828 | 11 | 837 | 0.0 | 1644 |
| Msa1059670 | MtrunA17_Chr7g0236121 | 62.546 | 817 | 266 | 12 | 15 | 813 | 13 | 807 | 0.0 | 1036 |
| Msa1059670 | MtrunA17_Chr7g0236201 | 61.566 | 830 | 250 | 11 | 15 | 828 | 13 | 789 | 0.0 | 1027 |
| Msa1059670 | MtrunA17_Chr7g0236641 | 59.437 | 853 | 312 | 10 | 1 | 828 | 1 | 844 | 0.0 | 994 |
| Msa1059670 | MtrunA17_Chr7g0236341 | 59.349 | 829 | 302 | 9 | 19 | 828 | 20 | 832 | 0.0 | 988 |
| Msa1059670 | MtrunA17_Chr7g0236691 | 59.905 | 838 | 307 | 8 | 1 | 828 | 1 | 819 | 0.0 | 980 |
| Msa1059670 | MtrunA17_Chr7g0236551 | 58.264 | 841 | 304 | 10 | 1 | 828 | 1 | 807 | 0.0 | 978 |
| Msa1059670 | MtrunA17_Chr7g0236651 | 58.313 | 830 | 298 | 11 | 8 | 828 | 6 | 796 | 0.0 | 965 |
| Msa1059670 | MtrunA17_Chr7g0236161 | 58.968 | 814 | 247 | 10 | 15 | 813 | 13 | 754 | 0.0 | 963 |
| Msa1059670 | MtrunA17_Chr7g0236561 | 58.495 | 824 | 314 | 8 | 26 | 828 | 7 | 823 | 0.0 | 954 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1059670 | AT1G11330.1 | 49.320 | 809 | 388 | 12 | 35 | 828 | 39 | 840 | 0.0 | 778 |
| Msa1059670 | AT1G11330.2 | 49.077 | 813 | 386 | 13 | 35 | 828 | 39 | 842 | 0.0 | 778 |
| Msa1059670 | AT1G11300.1 | 48.921 | 834 | 383 | 14 | 15 | 828 | 10 | 820 | 0.0 | 774 |
| Msa1059670 | AT1G11300.2 | 48.739 | 833 | 371 | 15 | 15 | 828 | 10 | 805 | 0.0 | 765 |
| Msa1059670 | AT1G11303.1 | 47.143 | 840 | 397 | 15 | 10 | 828 | 7 | 820 | 0.0 | 734 |
| Msa1059670 | AT1G11350.1 | 48.044 | 818 | 397 | 14 | 28 | 828 | 24 | 830 | 0.0 | 729 |
| Msa1059670 | AT4G21390.1 | 45.499 | 822 | 402 | 18 | 39 | 828 | 42 | 849 | 0.0 | 694 |
| Msa1059670 | AT1G11330.3 | 50.492 | 711 | 324 | 13 | 35 | 726 | 39 | 740 | 0.0 | 694 |
| Msa1059670 | AT1G61370.1 | 43.353 | 835 | 425 | 16 | 11 | 828 | 11 | 814 | 0.0 | 678 |
| Msa1059670 | AT1G61610.1 | 42.909 | 825 | 424 | 19 | 33 | 828 | 36 | 842 | 0.0 | 649 |
Find 140 sgRNAs with CRISPR-Local
Find 188 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGACTTTCGAGATCATC+CGG | 0.487705 | 7_2:+46405204 | Msa1059670:CDS |
| AAAATCTTGGAGTATTGAAA+AGG | 0.502539 | 7_2:+46404034 | Msa1059670:CDS |
| AAACGCTTTAGTATAATAGA+AGG | 0.447930 | 7_2:+46405571 | Msa1059670:CDS |
| AAACTCAACTCTTTGTTGTA+TGG | 0.310445 | 7_2:+46403451 | Msa1059670:CDS |
| AAACTTGGACAAGGAGGTTT+TGG | 0.261465 | 7_2:+46405058 | Msa1059670:CDS |
| AAAGAGAAGCTTCCGTTGGA+TGG | 0.488539 | 7_2:-46403774 | None:intergenic |
| AAATCAGACGTCTTCAGCTT+TGG | 0.376486 | 7_2:+46405903 | Msa1059670:CDS |
| AAATCAGATTGTGATCTTTA+TGG | 0.106758 | 7_2:+46404086 | Msa1059670:CDS |
| AACAATATTGTTGTATTGAA+CGG | 0.458584 | 7_2:+46403537 | Msa1059670:CDS |
| AACCAAGAAACAATCAAGAG+TGG | 0.602313 | 7_2:+46404177 | Msa1059670:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAACTTTAAGCTCAAATAG+TGG | + | chr7_2:46403363-46403382 | Msa1059670:CDS | 30.0% | |
| AAGAATCATTGATCAATGGT+TGG | - | chr7_2:46403491-46403510 | None:intergenic | 30.0% | |
| ACGGAAAGAAAAATGTTATC+TGG | + | chr7_2:46403556-46403575 | Msa1059670:CDS | 30.0% | |
| TCATGATGCTTGTAAATTTC+AGG | - | chr7_2:46403725-46403744 | None:intergenic | 30.0% | |
| TCTTTCCTGATAAAAACTTC+TGG | - | chr7_2:46403822-46403841 | None:intergenic | 30.0% | |
| ATGTCAGCTCTTTATCTTAA+TGG | + | chr7_2:46403903-46403922 | Msa1059670:CDS | 30.0% | |
| ATGTATGTTCTTAATTCGAC+AGG | + | chr7_2:46403999-46404018 | Msa1059670:CDS | 30.0% | |
| ATTGGTTGTATTTCTTGGAA+TGG | + | chr7_2:46404425-46404444 | Msa1059670:CDS | 30.0% | |
| GATATTCAACAATTCGAAAC+TGG | + | chr7_2:46404458-46404477 | Msa1059670:CDS | 30.0% | |
| GGGAGGATAAAAACTTATAA+CGG | - | chr7_2:46404644-46404663 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 46403249 | 46406409 | 46403249 | ID=Msa1059670;Name=Msa1059670 |
| chr7_2 | mRNA | 46403249 | 46406409 | 46403249 | ID=Msa1059670-mRNA-1;Parent=Msa1059670;Name=Msa1059670-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0.33|0|1|1|1|7|0|828 |
| chr7_2 | exon | 46403249 | 46404515 | 46403249 | ID=Msa1059670-mRNA-1:exon:8394;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46404674 | 46404778 | 46404674 | ID=Msa1059670-mRNA-1:exon:8395;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46404888 | 46405093 | 46404888 | ID=Msa1059670-mRNA-1:exon:8396;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46405171 | 46405381 | 46405171 | ID=Msa1059670-mRNA-1:exon:8397;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46405539 | 46405776 | 46405539 | ID=Msa1059670-mRNA-1:exon:8398;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46405857 | 46406007 | 46405857 | ID=Msa1059670-mRNA-1:exon:8399;Parent=Msa1059670-mRNA-1 |
| chr7_2 | exon | 46406101 | 46406409 | 46406101 | ID=Msa1059670-mRNA-1:exon:8400;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46403249 | 46404515 | 46403249 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46404674 | 46404778 | 46404674 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46404888 | 46405093 | 46404888 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46405171 | 46405381 | 46405171 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46405539 | 46405776 | 46405539 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46405857 | 46406007 | 46405857 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| chr7_2 | CDS | 46406101 | 46406409 | 46406101 | ID=Msa1059670-mRNA-1:cds;Parent=Msa1059670-mRNA-1 |
| Gene Sequence |
>Msa1059670 ATGGTTTTAAGCAAGTGCAAAAATTTGATCTCAGTTTTGAATTTCATCACATCATTACTATGTTGTTCTTTTTTTGTTCATGTTGTTGCCACACAACAAATCATCAAAGACTCCGAAACTTTAAGCTCAAATAGTGGAAATTTCACCTTAGGATTTTTCACCCCTCAAAATTCCACAAATCGCTATGTAGGAATTTGGTGCAAAACTCAACTCTTTGTTGTATGGGTTGCCAACAGAAACCAACCATTGATCAATGATTCTTCTGGAGTTCTTGAAATATCCAATGATAACAATATTGTTGTATTGAACGGAAAGAAAAATGTTATCTGGTCAACAAATTTGAACAATGTTTCAACAATCACCAATTCTAGTGTCACACTTTCAGATTATGGAAGTCTTGAATTGTTTGAAACAATTACAGAAAAAACCATATGGGCGAGTTCCGATCATCCTACAAATTCAATATTACCAAGCCTGAAATTTACAAGCATCATGACAATCACATCATGGAAAACACCTACTGATCCATCCAACGGAAGCTTCTCTTTAAGCATTGAACGGTTGAATATGCCAGAAGTTTTTATCAGGAAAGAAAATCGGCCATATTGGCGGAGTGGTCCTTGGAACAATCAAATCTTTATAGGGATAGAAGACATGTCAGCTCTTTATCTTAATGGTTTTCATTTCGGAAAAGATAGAACCGGAGGAACTCTTGATTTAGTCTTCAGAGCAGACGACTATGGACTTGTGATGTATGTTCTTAATTCGACAGGTCAAATGAATGAAAAATCTTGGAGTATTGAAAAGGAGGAATGGATGGATACATGGACTAATCAAAAATCAGATTGTGATCTTTATGGTTTTTGTGGTTCATTTGGAATTTGTAATTCAAAAGGGTCCCCAATTTGTCGCTGTTTGGAAGGGTTTGAACCAAGAAACAATCAAGAGTGGAATAAACAAAATTGGACTAATGGATGTGTTAGGAAGACGCCGTTGCAGTGTGAAAGTGCCAACAATCAAAATAAGAGCGCAAATGGTAATGAAGCAGTTAGTTTTATGAAATTGAGTCTGGTAAAAGTACCTGATTTTGCAGAATTGTCATCTGTTGAACAAGATGAATGCAAAAACCAGTGTTTGATGAATTGCTCTTGCACTGCATATTCATATGTAGCTGATATTGGTTGTATTTCTTGGAATGGAAATCTAATCGATATTCAACAATTCGAAACTGGAGGAACTGATCTATACATTCGTGTACCATCATCAGATAAAGGGAACAATGGAACAGTTATCATTACAGTTTCAGTACTAGTAGGAATTATCATATTTGCTACTGCGGCATATTTCATTTGGATAAAGGTTTCAAAGCCAGTGAGAAATAAGAAGAAACATAGAATTTTTCAATTCAACAAAATTGAAAAAGATGAAGAATACAAAACTGACAATGTAATTGGAGAACTGTCACAAGCAAAGCTACAAGAGCTATTACTATATAGTTTTGAAAAGCTTGCAATTGCCACAAACAACTTCCATTTATCCAATAAACTTGGACAAGGAGGTTTTGGTCCTGTATACAAGGGAATACTGCAAGATGGTAAGGAAATAGCGGTTAAAAGACTTTCGAGATCATCCGGACAAGGGTTAGAAGAATTCATGAATGAAGTAGTAGTGATTTCTAAGCTTCAGCACCGAAATCTTGTAAGACTTGTAGGCTGCTGCATTGAAGGAGACGAAAAGATGTTGATGTATGAGTTCATGCCAAATAGCAGTCTGGATGCATATGTTTTTGATCCATCAAGAAATAAACTTCTGGATTGGGAGAAACGCTTTAGTATAATAGAAGGAATAGCTCGTGGATTGCTTTATCTACACAGGGATTCCAGGCTAAAGATCATACATAGAGATATGAAAGCAAGTAATATCTTGCTAGACGAGAACCTGAATCCAAAAATATCTGACTTTGGTATGGCCAGAATCTTTGGAGTGAGTGAAGATCATGCCAATACTCAAAGGGTTGTCGGAACATATGGTTATATGGCTCCAGAGTATGCAATGCAAGGAGTATTTTCGGATAAATCAGACGTCTTCAGCTTTGGAGTTTTGCTAATAGAGATTGTTAGTGGAAGACGAAACTCAAGCTTTTATGAACATGAAAATTCTCTCACTCTCTTAGGATTTGCTTGGATTCAATGGAAGGAAGACAATATTTTATCTTTGATAGAACCAGAAATATATGATCATAGTAATCATAGGAGTATTTCGAGATGCATACATATAGGACTGTTATGTGTACAAGAATCGGCTGTGGATAGACCAACTATGGCTGCAGTGATTTCTATGCTTAACAGTGAAATTATGGACATTCCTCCTGCAAGGGAACCTGCATTCCTACTGAGGCAGAATATGTTGAATACAGTTTCTTCTGAAGAAAGAAATGAATTTTACTCCAATAATGCTTTGAGTATCACAGACATTCATGGAAGATAA |
| Protein sequence |
>Msa1059670 MVLSKCKNLISVLNFITSLLCCSFFVHVVATQQIIKDSETLSSNSGNFTLGFFTPQNSTNRYVGIWCKTQLFVVWVANRNQPLINDSSGVLEISNDNNIVVLNGKKNVIWSTNLNNVSTITNSSVTLSDYGSLELFETITEKTIWASSDHPTNSILPSLKFTSIMTITSWKTPTDPSNGSFSLSIERLNMPEVFIRKENRPYWRSGPWNNQIFIGIEDMSALYLNGFHFGKDRTGGTLDLVFRADDYGLVMYVLNSTGQMNEKSWSIEKEEWMDTWTNQKSDCDLYGFCGSFGICNSKGSPICRCLEGFEPRNNQEWNKQNWTNGCVRKTPLQCESANNQNKSANGNEAVSFMKLSLVKVPDFAELSSVEQDECKNQCLMNCSCTAYSYVADIGCISWNGNLIDIQQFETGGTDLYIRVPSSDKGNNGTVIITVSVLVGIIIFATAAYFIWIKVSKPVRNKKKHRIFQFNKIEKDEEYKTDNVIGELSQAKLQELLLYSFEKLAIATNNFHLSNKLGQGGFGPVYKGILQDGKEIAVKRLSRSSGQGLEEFMNEVVVISKLQHRNLVRLVGCCIEGDEKMLMYEFMPNSSLDAYVFDPSRNKLLDWEKRFSIIEGIARGLLYLHRDSRLKIIHRDMKASNILLDENLNPKISDFGMARIFGVSEDHANTQRVVGTYGYMAPEYAMQGVFSDKSDVFSFGVLLIEIVSGRRNSSFYEHENSLTLLGFAWIQWKEDNILSLIEPEIYDHSNHRSISRCIHIGLLCVQESAVDRPTMAAVISMLNSEIMDIPPAREPAFLLRQNMLNTVSSEERNEFYSNNALSITDIHGR* |
