AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0794700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794700 MtrunA17_Chr1g0169581 71.831 142 39 1 14 154 1 142 3.36e-64 192
Msa0794700 MtrunA17_Chr5g0434951 72.857 140 36 1 14 153 1 138 1.54e-63 191
Msa0794700 MtrunA17_Chr1g0169621 59.639 166 41 1 14 153 1 166 2.86e-57 176
Msa0794700 MtrunA17_Chr3g0111371 63.571 140 46 2 15 154 3 137 1.70e-56 173
Msa0794700 MtrunA17_Chr5g0396261 75.652 115 27 1 41 154 1 115 7.25e-51 158
Msa0794700 MtrunA17_Chr8g0392951 51.095 137 65 1 16 152 23 157 3.10e-37 125
Msa0794700 MtrunA17_Chr6g0482731 47.482 139 70 2 16 153 25 161 7.31e-34 117
Msa0794700 MtrunA17_Chr1g0161211 43.609 133 74 1 20 152 49 180 5.33e-30 107
Msa0794700 MtrunA17_Chr8g0391691 40.602 133 78 1 17 149 36 167 2.65e-27 100
Msa0794700 MtrunA17_Chr7g0256171 41.304 138 78 2 17 152 5 141 1.99e-26 97.4
Msa0794700 MtrunA17_Chr6g0459231 37.594 133 81 1 20 152 11 141 3.01e-26 96.7
Msa0794700 MtrunA17_Chr4g0033601 38.129 139 83 2 16 152 4 141 6.75e-26 95.9
Msa0794700 MtrunA17_Chr7g0245911 40.523 153 77 4 11 152 41 190 2.19e-25 95.9
Msa0794700 MtrunA17_Chr8g0351681 45.714 140 67 4 21 152 53 191 6.95e-25 94.7
Msa0794700 MtrunA17_Chr7g0225921 35.821 134 83 2 20 152 9 140 7.71e-23 87.8
Msa0794700 MtrunA17_Chr5g0440931 37.500 136 84 1 17 152 12 146 1.69e-22 87.4
Msa0794700 MtrunA17_Chr6g0460211 37.500 136 84 1 17 152 12 146 2.24e-22 87.0
Msa0794700 MtrunA17_Chr7g0254731 36.765 136 85 1 17 152 12 146 2.75e-22 86.7
Msa0794700 MtrunA17_Chr3g0093271 37.500 136 84 1 17 152 63 197 7.29e-22 87.0
Msa0794700 MtrunA17_Chr1g0166281 35.294 136 87 1 17 152 12 146 5.36e-21 83.6
Msa0794700 MtrunA17_Chr3g0116141 34.586 133 85 1 20 152 8 138 9.74e-21 82.4
Msa0794700 MtrunA17_Chr3g0116151 34.586 133 85 1 20 152 9 139 1.02e-20 82.4
Msa0794700 MtrunA17_Chr1g0188171 35.878 131 83 1 22 152 17 146 1.03e-20 82.8
Msa0794700 MtrunA17_Chr4g0044961 38.129 139 83 2 16 154 56 191 1.47e-20 83.6
Msa0794700 MtrunA17_Chr5g0402331 39.286 140 78 4 16 152 47 182 2.32e-20 82.8
Msa0794700 MtrunA17_Chr4g0062221 28.571 140 99 1 16 154 10 149 5.90e-20 81.3
Msa0794700 MtrunA17_Chr5g0402361 39.286 140 78 4 16 152 219 354 3.29e-19 82.4
Msa0794700 MtrunA17_Chr5g0402361 35.766 137 86 1 16 152 47 181 1.38e-17 78.2
Msa0794700 MtrunA17_Chr4g0064231 35.503 169 91 4 1 152 49 216 4.78e-19 80.1
Msa0794700 MtrunA17_Chr8g0366401 28.986 138 96 1 17 152 17 154 1.66e-18 77.4
Msa0794700 MtrunA17_Chr7g0245371 37.063 143 79 3 21 152 28 170 1.07e-17 75.5
Msa0794700 MtrunA17_Chr4g0057651 33.824 136 88 1 17 152 6 139 1.82e-17 73.9
Msa0794700 MtrunA17_Chr8g0373691 34.074 135 84 3 21 153 57 188 4.46e-17 74.3
Msa0794700 MtrunA17_Chr4g0057641 33.333 138 90 1 15 152 4 139 6.57e-17 72.4
Msa0794700 MtrunA17_Chr4g0057661 33.333 138 90 1 15 152 4 139 6.71e-17 72.4
Msa0794700 MtrunA17_Chr1g0185001 31.618 136 91 1 17 152 12 145 1.12e-16 72.0
Msa0794700 MtrunA17_Chr2g0329371 34.286 140 89 2 16 152 63 202 1.33e-16 73.6
Msa0794700 MtrunA17_Chr8g0387671 34.532 139 78 4 21 154 375 505 1.76e-15 72.4
Msa0794700 MtrunA17_Chr5g0397101 35.252 139 77 4 21 154 375 505 1.88e-15 72.4
Msa0794700 MtrunA17_Chr5g0434981 54.688 64 28 1 20 83 13 75 9.13e-15 65.5
Msa0794700 MtrunA17_Chr7g0256701 30.714 140 93 3 17 152 73 212 9.61e-15 68.6
Msa0794700 MtrunA17_Chr8g0368681 28.058 139 99 1 17 154 12 150 1.00e-14 67.4
Msa0794700 MtrunA17_Chr5g0444171 35.036 137 76 4 21 152 401 529 1.20e-14 70.1
Msa0794700 MtrunA17_Chr3g0121191 31.655 139 84 2 14 152 90 217 1.30e-14 68.6
Msa0794700 MtrunA17_Chr3g0098951 34.783 138 82 2 21 152 392 527 2.44e-14 69.3
Msa0794700 MtrunA17_Chr4g0074811 33.582 134 82 3 21 152 400 528 2.99e-14 68.9
Msa0794700 MtrunA17_Chr8g0375741 47.692 65 33 1 17 81 6 69 6.07e-14 63.2
Msa0794700 MtrunA17_Chr7g0274101 30.147 136 94 1 17 152 12 146 8.36e-14 64.7
Msa0794700 MtrunA17_Chr6g0450921 29.710 138 96 1 17 154 29 165 1.30e-13 64.7
Msa0794700 MtrunA17_Chr7g0245231 28.676 136 96 1 17 152 30 164 4.42e-13 63.2
Msa0794700 MtrunA17_Chr5g0441691 30.657 137 88 3 20 154 376 507 6.68e-13 65.1
Msa0794700 MtrunA17_Chr1g0157131 31.884 138 85 3 20 154 382 513 2.10e-12 63.5
Msa0794700 MtrunA17_Chr1g0160151 32.624 141 80 3 21 152 26 160 4.38e-12 60.5
Msa0794700 MtrunA17_Chr5g0398751 29.851 134 90 3 22 154 17 147 1.30e-11 58.9
Msa0794700 MtrunA17_Chr7g0242561 32.877 146 93 3 10 152 351 494 2.69e-11 60.5
Msa0794700 MtrunA17_Chr0c02g0489761 30.216 139 84 4 21 154 387 517 3.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0794700 AT1G18210.2 48.592 142 68 2 11 152 17 153 3.22e-38 128
Msa0794700 AT1G18210.1 48.592 142 68 2 11 152 17 153 3.22e-38 128
Msa0794700 AT1G73630.1 43.624 149 79 2 5 153 8 151 2.00e-35 121
Msa0794700 AT5G37770.1 46.528 144 74 2 13 153 13 156 3.62e-34 117
Msa0794700 AT5G37770.2 46.528 144 74 2 13 153 13 156 3.62e-34 117
Msa0794700 AT1G66400.1 44.681 141 77 1 13 152 11 151 1.18e-32 114
Msa0794700 AT5G17470.1 43.165 139 74 2 18 153 3 139 5.78e-32 111
Msa0794700 AT2G36180.1 43.885 139 72 2 18 152 1 137 1.24e-29 105
Msa0794700 AT1G24620.1 41.606 137 79 1 17 153 37 172 2.10e-28 103
Msa0794700 AT3G07490.1 42.029 138 77 2 17 152 5 141 4.11e-27 99.8
Msa0794700 AT1G05990.1 39.130 138 82 1 17 152 5 142 1.02e-25 95.9
Msa0794700 AT2G15680.1 39.130 138 81 1 16 153 49 183 9.08e-25 94.4
Msa0794700 AT4G03290.1 35.915 142 87 2 17 154 5 146 7.28e-23 88.6
Msa0794700 AT1G66410.1 37.500 136 84 1 17 152 12 146 1.16e-22 88.2
Msa0794700 AT5G37780.1 37.500 136 84 1 17 152 12 146 1.16e-22 88.2
Msa0794700 AT5G21274.1 38.235 136 83 1 17 152 12 146 1.35e-22 87.8
Msa0794700 AT3G03400.1 37.313 134 79 2 15 145 3 134 1.40e-22 87.4
Msa0794700 AT4G12860.1 37.681 138 83 2 17 152 5 141 2.44e-22 87.4
Msa0794700 AT3G56800.1 37.500 136 84 1 17 152 12 146 2.96e-22 87.0
Msa0794700 AT2G41110.1 37.500 136 84 1 17 152 12 146 2.96e-22 87.0
Msa0794700 AT2G27030.1 37.500 136 84 1 17 152 12 146 2.96e-22 87.0
Msa0794700 AT3G43810.1 37.500 136 84 1 17 152 12 146 3.09e-22 87.0
Msa0794700 AT2G27030.3 37.500 136 84 1 17 152 12 146 5.69e-22 87.0
Msa0794700 AT3G43810.2 38.168 131 80 1 22 152 17 146 9.79e-22 87.4
Msa0794700 AT2G43290.1 35.915 142 85 2 17 152 65 206 1.43e-21 87.0
Msa0794700 AT3G10190.1 39.130 138 80 3 17 152 70 205 3.10e-21 85.9
Msa0794700 AT4G37010.2 33.557 149 90 3 12 154 22 167 5.05e-20 81.6
Msa0794700 AT4G37010.1 33.557 149 90 3 12 154 18 163 5.41e-20 81.6
Msa0794700 AT3G59440.1 35.821 134 82 2 21 152 55 186 1.83e-19 80.9
Msa0794700 AT3G50360.1 32.609 138 92 1 17 154 27 163 1.96e-19 80.1
Msa0794700 AT2G41110.2 35.811 148 82 3 17 152 12 158 2.39e-19 79.7
Msa0794700 AT3G22930.1 35.115 131 84 1 22 152 40 169 3.42e-19 79.7
Msa0794700 AT1G32250.1 28.873 142 96 1 16 152 15 156 1.13e-18 78.2
Msa0794700 AT5G37780.2 34.247 146 80 2 22 152 17 161 1.18e-18 78.2
Msa0794700 AT1G66410.4 37.838 111 68 1 42 152 1 110 2.84e-18 75.9
Msa0794700 AT1G66410.3 37.838 111 68 1 42 152 1 110 2.84e-18 75.9
Msa0794700 AT5G37780.4 36.066 122 77 1 31 152 13 133 3.82e-18 75.9
Msa0794700 AT1G66410.2 36.066 122 77 1 31 152 36 156 5.88e-18 76.3
Msa0794700 AT3G43810.4 37.838 111 68 1 42 152 1 110 7.90e-18 74.7
Msa0794700 AT3G43810.3 37.838 111 68 1 42 152 1 110 7.90e-18 74.7
Msa0794700 AT4G14640.1 33.588 131 86 1 22 152 18 147 1.02e-17 75.5
Msa0794700 AT1G76650.1 34.783 138 88 1 15 152 41 176 1.03e-17 75.9
Msa0794700 AT1G76650.3 34.783 138 88 1 15 152 41 176 1.03e-17 75.9
Msa0794700 AT5G37780.3 36.066 122 77 1 31 152 52 172 1.16e-17 75.9
Msa0794700 AT2G41410.1 31.169 154 102 3 2 153 57 208 3.16e-17 75.5
Msa0794700 AT5G42380.1 32.847 137 92 0 16 152 48 184 4.10e-17 74.7
Msa0794700 AT1G18530.1 28.058 139 99 1 17 154 7 145 4.40e-17 73.9
Msa0794700 AT3G50770.1 30.282 142 95 3 16 153 63 204 9.48e-17 73.9
Msa0794700 AT1G76650.2 34.783 138 78 2 15 152 41 166 4.28e-16 71.6
Msa0794700 AT2G41100.4 33.108 148 84 2 22 154 17 164 4.77e-16 73.9
Msa0794700 AT2G41100.4 31.757 148 85 2 22 153 106 253 1.31e-14 69.7
Msa0794700 AT2G41100.1 33.108 148 84 2 22 154 17 164 4.77e-16 73.9
Msa0794700 AT2G41100.1 31.757 148 85 2 22 153 106 253 1.31e-14 69.7
Msa0794700 AT4G23650.1 33.813 139 79 4 21 154 387 517 4.82e-16 74.3
Msa0794700 AT4G04695.1 35.507 138 76 4 20 152 339 468 6.96e-16 73.9
Msa0794700 AT4G20780.1 32.450 151 83 2 21 152 33 183 2.36e-15 70.1
Msa0794700 AT2G41100.7 31.757 148 85 2 22 153 71 218 9.18e-15 70.1
Msa0794700 AT2G41100.7 31.008 129 74 2 41 154 1 129 6.30e-11 59.3
Msa0794700 AT2G41100.6 31.757 148 85 2 22 153 71 218 9.18e-15 70.1
Msa0794700 AT2G41100.6 31.008 129 74 2 41 154 1 129 6.30e-11 59.3
Msa0794700 AT2G41100.3 31.757 148 85 2 22 153 71 218 9.18e-15 70.1
Msa0794700 AT2G41100.3 31.008 129 74 2 41 154 1 129 6.30e-11 59.3
Msa0794700 AT5G17480.1 55.556 63 27 1 21 83 13 74 1.16e-14 65.5
Msa0794700 AT2G41090.1 34.351 131 82 2 22 152 17 143 1.46e-14 68.2
Msa0794700 AT4G04700.1 34.074 135 82 3 20 152 339 468 2.01e-14 69.7
Msa0794700 AT3G03410.1 30.303 132 82 2 21 152 6 127 4.10e-14 65.5
Msa0794700 AT3G03000.1 30.147 136 95 0 17 152 20 155 4.39e-14 66.2
Msa0794700 AT3G25600.1 27.338 139 100 1 17 154 12 150 4.61e-14 65.9
Msa0794700 AT4G14640.2 31.148 122 83 1 31 152 9 129 9.51e-14 64.7
Msa0794700 AT1G61950.1 35.294 136 79 3 20 152 407 536 1.76e-13 67.0
Msa0794700 AT1G76640.1 31.655 139 94 1 15 152 20 158 2.13e-13 64.3
Msa0794700 AT4G04740.4 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT4G04740.6 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT4G04740.5 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT4G04740.3 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT4G04740.1 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT4G04740.7 35.507 138 76 4 20 152 376 505 2.79e-13 66.6
Msa0794700 AT3G20410.2 35.766 137 75 4 21 152 400 528 3.90e-13 66.2
Msa0794700 AT3G20410.1 35.766 137 75 4 21 152 400 528 3.90e-13 66.2
Msa0794700 AT3G03430.1 50.794 63 30 1 21 83 13 74 6.01e-13 61.2
Msa0794700 AT4G04720.2 34.783 138 77 4 20 152 387 516 6.79e-13 65.5
Msa0794700 AT4G04720.1 34.783 138 77 4 20 152 387 516 6.79e-13 65.5
Msa0794700 AT4G21940.2 34.783 138 77 4 20 152 409 538 8.02e-13 65.1
Msa0794700 AT4G21940.1 34.783 138 77 4 20 152 409 538 9.15e-13 65.1
Msa0794700 AT5G19360.1 32.353 136 85 3 21 154 377 507 1.16e-12 64.7
Msa0794700 AT3G01830.1 32.857 140 87 5 16 152 10 145 1.28e-12 62.0
Msa0794700 AT1G50700.1 33.577 137 78 4 21 152 382 510 2.60e-12 63.9
Msa0794700 AT1G62820.1 29.167 144 95 4 15 154 8 148 6.08e-12 60.1
Msa0794700 AT5G12180.1 31.618 136 86 3 21 154 382 512 6.45e-12 62.8
Msa0794700 AT3G51850.1 32.090 134 85 2 21 152 363 492 1.52e-11 61.6
Msa0794700 AT5G04870.1 29.927 137 86 4 21 154 459 588 1.95e-11 61.2
Msa0794700 AT5G44460.1 32.639 144 84 3 21 152 32 174 2.56e-11 59.3
Msa0794700 AT1G74740.1 35.294 136 80 5 20 152 367 497 2.59e-11 60.8
Msa0794700 AT3G51920.1 28.676 136 96 1 17 152 12 146 3.10e-11 58.5
Msa0794700 AT1G76040.1 33.088 136 82 3 22 154 183 312 3.43e-11 60.1
Msa0794700 AT1G76040.4 33.088 136 82 3 22 154 183 312 3.43e-11 60.1
Msa0794700 AT1G76040.2 33.088 136 82 3 22 154 421 550 3.65e-11 60.5
Msa0794700 AT1G12310.1 28.472 144 96 4 15 154 8 148 6.56e-11 57.4
Msa0794700 AT1G21550.1 33.793 145 77 4 21 152 14 152 7.61e-11 57.4

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACAATGTGCAGTTCTTT+TGG 0.251922 5_3:+6621567 Msa0794690:intron
AAGAAATGATTTCGTATTCT+TGG 0.267268 5_3:-6621896 None:intergenic
CACATTGTTCTTAATAAACT+TGG 0.346651 5_3:-6621555 Msa0794700:CDS
TACTTCATCAGATGTCGTTT+TGG 0.347899 5_3:+6621752 Msa0794690:intron
TCATACCGTAATCACCGTTT+TGG 0.378053 5_3:+6621871 Msa0794690:intron
AAAGAAATGCTTTCAACGCT+TGG 0.389101 5_3:-6621777 Msa0794700:CDS
TGGAACCAAAACGGTGATTA+CGG 0.396473 5_3:-6621876 Msa0794700:CDS
CGACGTTGCTTATCATTCTC+AGG 0.449084 5_3:+6621508 Msa0794690:intron
AGCGTTGAAAGCATTTCTTT+CGG 0.471052 5_3:+6621780 Msa0794690:intron
TGACGGAAACATTAACTTTG+AGG 0.498713 5_3:-6621475 Msa0794700:CDS
TCGTATTCTTGGAACCAAAA+CGG 0.526744 5_3:-6621885 Msa0794700:CDS
TGAGGAGTTCAAGAAGATGA+TGG 0.534080 5_3:-6621457 Msa0794700:CDS
CGGATTGATCAGAACGGCGA+TGG 0.536019 5_3:-6621714 Msa0794700:CDS
TTCAACAAGTTCGACAAGAA+CGG 0.554513 5_3:-6621828 Msa0794700:CDS
GATTTCCACTGCACTCATGT+TGG 0.557381 5_3:-6621666 Msa0794700:CDS
TGATGAAGTAAAACGCATGA+AGG 0.558820 5_3:-6621739 Msa0794700:CDS
CGTCCTTGTCGAGGTCGTAA+AGG 0.575303 5_3:+6621601 Msa0794690:intron
AAGTAAAACGCATGAAGGAA+CGG 0.584140 5_3:-6621734 Msa0794700:CDS
AAGTTCGACAAGAACGGCGA+CGG 0.611394 5_3:-6621822 Msa0794700:CDS
CGACCTTTACGACCTCGACA+AGG 0.613996 5_3:-6621604 Msa0794700:CDS
AACGTCGATGTTGATGATGA+CGG 0.629007 5_3:-6621492 Msa0794700:CDS
CATTCTCAGGTAGTCACCGA+GGG 0.631930 5_3:+6621521 Msa0794690:intron
CTTGGCGAGAAGTACTCCCT+CGG 0.634170 5_3:-6621537 Msa0794700:CDS
AGATGAGGCCGTCCTTGTCG+AGG 0.636799 5_3:+6621592 Msa0794690:intron
CATCACCAACATGAGTGCAG+TGG 0.646931 5_3:+6621661 Msa0794690:intron
AAGGAACGGATTGATCAGAA+CGG 0.690029 5_3:-6621720 Msa0794700:CDS
CTTTACGACCTCGACAAGGA+CGG 0.703036 5_3:-6621600 Msa0794700:CDS
TCATTCTCAGGTAGTCACCG+AGG 0.703464 5_3:+6621520 Msa0794690:intron
TGATTACGGTATGAACGACG+AGG 0.775749 5_3:-6621862 Msa0794700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CACATTGTTCTTAATAAACT+TGG - chr5_3:6621785-6621804 Msa0794700:CDS 25.0%
TCGTATTCTTGGAACCAAAA+CGG - chr5_3:6621455-6621474 Msa0794700:CDS 35.0%
TTCAACAAGTTCGACAAGAA+CGG - chr5_3:6621512-6621531 Msa0794700:CDS 35.0%
!! AGCGTTGAAAGCATTTCTTT+CGG + chr5_3:6621563-6621582 Msa0794690:intron 35.0%
AAAGAAATGCTTTCAACGCT+TGG - chr5_3:6621563-6621582 Msa0794700:CDS 35.0%
!! TACTTCATCAGATGTCGTTT+TGG + chr5_3:6621591-6621610 Msa0794690:intron 35.0%
TGATGAAGTAAAACGCATGA+AGG - chr5_3:6621601-6621620 Msa0794700:CDS 35.0%
AAGTAAAACGCATGAAGGAA+CGG - chr5_3:6621606-6621625 Msa0794700:CDS 35.0%
! AGAACAATGTGCAGTTCTTT+TGG + chr5_3:6621776-6621795 Msa0794690:intron 35.0%
TGACGGAAACATTAACTTTG+AGG - chr5_3:6621865-6621884 Msa0794700:CDS 35.0%
TGGAACCAAAACGGTGATTA+CGG - chr5_3:6621464-6621483 Msa0794700:CDS 40.0%
! TCATACCGTAATCACCGTTT+TGG + chr5_3:6621472-6621491 Msa0794690:intron 40.0%
!! AAGGAACGGATTGATCAGAA+CGG - chr5_3:6621620-6621639 Msa0794700:CDS 40.0%
!! AACGTCGATGTTGATGATGA+CGG - chr5_3:6621848-6621867 Msa0794700:CDS 40.0%
TGAGGAGTTCAAGAAGATGA+TGG - chr5_3:6621883-6621902 Msa0794700:CDS 40.0%
TGATTACGGTATGAACGACG+AGG - chr5_3:6621478-6621497 Msa0794700:CDS 45.0%
GATTTCCACTGCACTCATGT+TGG - chr5_3:6621674-6621693 Msa0794700:CDS 45.0%
!! GCAGTTCTTTTGGAGAGATG+AGG + chr5_3:6621766-6621785 Msa0794690:intron 45.0%
CGACGTTGCTTATCATTCTC+AGG + chr5_3:6621835-6621854 Msa0794690:intron 45.0%
AAGTTCGACAAGAACGGCGA+CGG - chr5_3:6621518-6621537 Msa0794700:CDS 50.0%
CATCACCAACATGAGTGCAG+TGG + chr5_3:6621682-6621701 Msa0794690:intron 50.0%
! GTGAAGCTCGTCGTCTTTTC+TGG + chr5_3:6621708-6621727 Msa0794690:intron 50.0%
CTTTACGACCTCGACAAGGA+CGG - chr5_3:6621740-6621759 Msa0794700:CDS 50.0%
CATTCTCAGGTAGTCACCGA+GGG + chr5_3:6621822-6621841 Msa0794690:intron 50.0%
TCATTCTCAGGTAGTCACCG+AGG + chr5_3:6621823-6621842 Msa0794690:intron 50.0%
CGGATTGATCAGAACGGCGA+TGG - chr5_3:6621626-6621645 Msa0794700:CDS 55.0%
CGACCTTTACGACCTCGACA+AGG - chr5_3:6621736-6621755 Msa0794700:CDS 55.0%
CGTCCTTGTCGAGGTCGTAA+AGG + chr5_3:6621742-6621761 Msa0794690:intron 55.0%
!! CTTGGCGAGAAGTACTCCCT+CGG - chr5_3:6621803-6621822 Msa0794700:CDS 55.0%
AGATGAGGCCGTCCTTGTCG+AGG + chr5_3:6621751-6621770 Msa0794690:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 6621449 6621913 6621449 ID=Msa0794700;Name=Msa0794700
chr5_3 mRNA 6621449 6621913 6621449 ID=Msa0794700-mRNA-1;Parent=Msa0794700;Name=Msa0794700-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|154
chr5_3 exon 6621449 6621913 6621449 ID=Msa0794700-mRNA-1:exon:2681;Parent=Msa0794700-mRNA-1
chr5_3 CDS 6621449 6621913 6621449 ID=Msa0794700-mRNA-1:cds;Parent=Msa0794700-mRNA-1
Gene Sequence

>Msa0794700

ATGATTTCGTATTCTTGGAACCAAAACGGTGATTACGGTATGAACGACGAGGTGTGTAACATCTTCAACAAGTTCGACAAGAACGGCGACGGAAAAATCTCTCTCTTAGATCCGAAAGAAATGCTTTCAACGCTTGGATCCAAAACGACATCTGATGAAGTAAAACGCATGAAGGAACGGATTGATCAGAACGGCGATGGTTACATTGATCTCAAAGAATTTGCTGATTTCCACTGCACTCATGTTGGTGATGCTGCCAGAAAAGACGACGAGCTTCACGATGCTATCGACCTTTACGACCTCGACAAGGACGGCCTCATCTCTCCAAAAGAACTGCACATTGTTCTTAATAAACTTGGCGAGAAGTACTCCCTCGGTGACTACCTGAGAATGATAAGCAACGTCGATGTTGATGATGACGGAAACATTAACTTTGAGGAGTTCAAGAAGATGATGGCTCGTTGA
Protein sequence

>Msa0794700

MISYSWNQNGDYGMNDEVCNIFNKFDKNGDGKISLLDPKEMLSTLGSKTTSDEVKRMKERIDQNGDGYIDLKEFADFHCTHVGDAARKDDELHDAIDLYDLDKDGLISPKELHIVLNKLGEKYSLGDYLRMISNVDVDDDGNINFEEFKKMMAR*