AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0900910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0900910 MtrunA17_Chr6g0487501 39.496 119 65 2 85 196 33 151 9.03e-20 82.4
Msa0900910 MtrunA17_Chr3g0092521 32.919 161 96 5 53 209 67 219 4.73e-19 82.8
Msa0900910 MtrunA17_Chr4g0060621 30.675 163 101 6 53 211 64 218 4.64e-16 74.7
Msa0900910 MtrunA17_Chr4g0064221 27.528 178 106 8 53 218 164 330 8.55e-12 63.5
Msa0900910 MtrunA17_Chr6g0487861 41.096 73 43 0 53 125 9 81 1.17e-11 58.9
Msa0900910 MtrunA17_Chr6g0487691 41.096 73 43 0 53 125 9 81 1.17e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0900910 AT1G74560.1 33.939 165 97 5 53 212 69 226 1.33e-20 87.4
Msa0900910 AT1G74560.3 33.566 143 86 3 53 193 69 204 6.12e-18 80.5
Msa0900910 AT1G18800.1 30.675 163 99 5 53 209 65 219 2.67e-17 78.6

Find 33 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTACATGGATTCTGGTTTA+AGG 0.177881 6_1:-97150596 Msa0900910:CDS
GATTATTATTTGTTTGATTA+TGG 0.226047 6_1:-97149969 Msa0900910:three_prime_UTR
CAGATGCATTTGAGATGTTT+TGG 0.252559 6_1:-97150192 Msa0900910:CDS
TTTCGCGCTTACATGGATTC+TGG 0.322014 6_1:-97150603 Msa0900910:CDS
ATTCTGGTTTAAGGCGATTA+AGG 0.325501 6_1:-97150587 Msa0900910:CDS
TAAATTAAATGTTCTTTGTT+TGG 0.338500 6_1:-97149940 Msa0900910:three_prime_UTR
CTGAATCTATTGCATTCAAC+TGG 0.388969 6_1:+97150545 None:intergenic
CTCTCTTCACAAACAATGTC+GGG 0.397057 6_1:+97150331 None:intergenic
TTCAACTGGCGACCAATGTC+TGG 0.403365 6_1:+97150559 None:intergenic
GAACATATTTCGCGCTTACA+TGG 0.441207 6_1:-97150610 Msa0900910:CDS
TGAAGAGAGAGATGAGGATG+AGG 0.446237 6_1:-97150317 Msa0900910:CDS
CACGTTCAATCTTCTCCACC+CGG 0.468969 6_1:+97150689 None:intergenic
GTGGTTTGAAATTGAGATGC+GGG 0.475190 6_1:-97150970 Msa0900910:CDS
AATAAATGTGGTTATTATTG+AGG 0.481101 6_1:-97149884 None:intergenic
GGGATGGATGTTTCTGATGA+GGG 0.503789 6_1:-97150996 Msa0900910:CDS
CTGAATCTTCATGCATACAT+TGG 0.523603 6_1:-97150366 Msa0900910:CDS
AGGGATGGATGTTTCTGATG+AGG 0.538812 6_1:-97150997 None:intergenic
TCTCTCTTCACAAACAATGT+CGG 0.541509 6_1:+97150330 None:intergenic
AGAGAGAGATGAGGATGAGG+AGG 0.543196 6_1:-97150314 Msa0900910:CDS
ATTATTTGTTTGAATAAATG+TGG 0.560942 6_1:-97149896 Msa0900910:three_prime_UTR
ATGTTTCTGATGAGGGAAGG+TGG 0.567771 6_1:-97150989 Msa0900910:CDS
CAACTCTTCTTCATCAGATG+TGG 0.573742 6_1:+97150234 None:intergenic
GATGAAGAAGAGTTGAGTGA+TGG 0.593224 6_1:-97150226 Msa0900910:CDS
TGGATGTTTCTGATGAGGGA+AGG 0.596404 6_1:-97150992 Msa0900910:CDS
GACACAGAGGATGAAGTTGA+TGG 0.599003 6_1:-97150133 Msa0900910:CDS
TCACCTTGTAGATACTTCAA+AGG 0.607832 6_1:+97150163 None:intergenic
ATTAAGGCTCATCCAGACAT+TGG 0.614087 6_1:-97150571 Msa0900910:CDS
GATCCTTTGAAGTATCTACA+AGG 0.624256 6_1:-97150166 Msa0900910:CDS
GGTGGTTTGAAATTGAGATG+CGG 0.640779 6_1:-97150971 Msa0900910:CDS
TGTTTGTGAAGAGAGAGATG+AGG 0.664206 6_1:-97150323 Msa0900910:CDS
AAAACCAAGAAAATATGATG+AGG 0.665852 6_1:-97150947 Msa0900910:CDS
GATGAGGATGAGGAGGACGA+CGG 0.699737 6_1:-97150307 Msa0900910:CDS
AGGTGATGATGATGACACAG+AGG 0.802099 6_1:-97150146 Msa0900910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGTTAGTTTATTTAATAT+GGG + chr6_1:97150115-97150134 None:intergenic 10.0%
!!! TTAAGTTAGTTTATTTAATA+TGG + chr6_1:97150116-97150135 None:intergenic 10.0%
!!! TAAGTTAGTTTATTTAATAT+GGG + chr6_1:97150115-97150134 None:intergenic 10.0%
!!! TTAAGTTAGTTTATTTAATA+TGG + chr6_1:97150116-97150135 None:intergenic 10.0%
!! TCATTAAAAAAAAAAACACT+TGG + chr6_1:97149966-97149985 None:intergenic 15.0%
!! AATAAAATCAAGGATTTATA+AGG + chr6_1:97150081-97150100 None:intergenic 15.0%
!!! TTTTTCATATACAATTTCAA+TGG + chr6_1:97150384-97150403 None:intergenic 15.0%
!!! TATTAGTTGTTTTTTGATTA+TGG - chr6_1:97150858-97150877 Msa0900910:intron 15.0%
!! GATTATTATTTGTTTGATTA+TGG - chr6_1:97150910-97150929 Msa0900910:intron 15.0%
!! TAAATTAAATGTTCTTTGTT+TGG - chr6_1:97150939-97150958 Msa0900910:CDS 15.0%
!! ATTATTTGTTTGAATAAATG+TGG - chr6_1:97150983-97151002 Msa0900910:CDS 15.0%
!! TCATTAAAAAAAAAAACACT+TGG + chr6_1:97149966-97149985 None:intergenic 15.0%
!! AATAAAATCAAGGATTTATA+AGG + chr6_1:97150081-97150100 None:intergenic 15.0%
!!! TTTTTCATATACAATTTCAA+TGG + chr6_1:97150384-97150403 None:intergenic 15.0%
!!! TATTAGTTGTTTTTTGATTA+TGG - chr6_1:97150858-97150877 Msa0900910:intron 15.0%
!! GATTATTATTTGTTTGATTA+TGG - chr6_1:97150910-97150929 Msa0900910:intron 15.0%
!! TAAATTAAATGTTCTTTGTT+TGG - chr6_1:97150939-97150958 Msa0900910:CDS 15.0%
!! ATTATTTGTTTGAATAAATG+TGG - chr6_1:97150983-97151002 Msa0900910:CDS 15.0%
!! AAAAAAAAAACACTTGGATA+CGG + chr6_1:97149960-97149979 None:intergenic 20.0%
!!! TATTATTATTTTAGACTCTC+CGG - chr6_1:97150171-97150190 Msa0900910:CDS 20.0%
!!! GAAAATGAATATTTTTCCAA+TGG - chr6_1:97150443-97150462 Msa0900910:CDS 20.0%
!! AAAAAAAAAACACTTGGATA+CGG + chr6_1:97149960-97149979 None:intergenic 20.0%
!!! TATTATTATTTTAGACTCTC+CGG - chr6_1:97150171-97150190 Msa0900910:CDS 20.0%
!!! GAAAATGAATATTTTTCCAA+TGG - chr6_1:97150443-97150462 Msa0900910:CDS 20.0%
! AAAACCAAGAAAATATGATG+AGG - chr6_1:97149932-97149951 Msa0900910:three_prime_UTR 25.0%
! CTTAGAGTTCAATAAAATCA+AGG + chr6_1:97150091-97150110 None:intergenic 25.0%
!!! ATTATTATTTTAGACTCTCC+GGG - chr6_1:97150172-97150191 Msa0900910:CDS 25.0%
! TATGAAAAAGATTCAGAATC+AGG - chr6_1:97150395-97150414 Msa0900910:CDS 25.0%
! GAACCAATTGAAAAAACTTT+GGG + chr6_1:97150621-97150640 None:intergenic 25.0%
! TGAACCAATTGAAAAAACTT+TGG + chr6_1:97150622-97150641 None:intergenic 25.0%
! AAAACCAAGAAAATATGATG+AGG - chr6_1:97149932-97149951 Msa0900910:three_prime_UTR 25.0%
! CTTAGAGTTCAATAAAATCA+AGG + chr6_1:97150091-97150110 None:intergenic 25.0%
!!! ATTATTATTTTAGACTCTCC+GGG - chr6_1:97150172-97150191 Msa0900910:CDS 25.0%
! TATGAAAAAGATTCAGAATC+AGG - chr6_1:97150395-97150414 Msa0900910:CDS 25.0%
! GAACCAATTGAAAAAACTTT+GGG + chr6_1:97150621-97150640 None:intergenic 25.0%
! TGAACCAATTGAAAAAACTT+TGG + chr6_1:97150622-97150641 None:intergenic 25.0%
! GGTACCTCATCATATTTTCT+TGG + chr6_1:97149939-97149958 None:intergenic 35.0%
!! CTTACATGGATTCTGGTTTA+AGG - chr6_1:97150283-97150302 Msa0900910:CDS 35.0%
!! ATTCTGGTTTAAGGCGATTA+AGG - chr6_1:97150292-97150311 Msa0900910:CDS 35.0%
CTGAATCTATTGCATTCAAC+TGG + chr6_1:97150337-97150356 None:intergenic 35.0%
CTGAATCTTCATGCATACAT+TGG - chr6_1:97150513-97150532 Msa0900910:CDS 35.0%
TCTCTCTTCACAAACAATGT+CGG + chr6_1:97150552-97150571 None:intergenic 35.0%
CCAATTGAAAAAACTTTGGG+AGG + chr6_1:97150618-97150637 None:intergenic 35.0%
!!! CCTCCCAAAGTTTTTTCAAT+TGG - chr6_1:97150615-97150634 Msa0900910:CDS 35.0%
!! CAGATGCATTTGAGATGTTT+TGG - chr6_1:97150687-97150706 Msa0900910:CDS 35.0%
!! GATCCTTTGAAGTATCTACA+AGG - chr6_1:97150713-97150732 Msa0900910:intron 35.0%
TCACCTTGTAGATACTTCAA+AGG + chr6_1:97150719-97150738 None:intergenic 35.0%
! GGTACCTCATCATATTTTCT+TGG + chr6_1:97149939-97149958 None:intergenic 35.0%
!! CTTACATGGATTCTGGTTTA+AGG - chr6_1:97150283-97150302 Msa0900910:CDS 35.0%
!! ATTCTGGTTTAAGGCGATTA+AGG - chr6_1:97150292-97150311 Msa0900910:CDS 35.0%
CTGAATCTATTGCATTCAAC+TGG + chr6_1:97150337-97150356 None:intergenic 35.0%
CTGAATCTTCATGCATACAT+TGG - chr6_1:97150513-97150532 Msa0900910:CDS 35.0%
TCTCTCTTCACAAACAATGT+CGG + chr6_1:97150552-97150571 None:intergenic 35.0%
CCAATTGAAAAAACTTTGGG+AGG + chr6_1:97150618-97150637 None:intergenic 35.0%
!!! CCTCCCAAAGTTTTTTCAAT+TGG - chr6_1:97150615-97150634 Msa0900910:CDS 35.0%
!! CAGATGCATTTGAGATGTTT+TGG - chr6_1:97150687-97150706 Msa0900910:CDS 35.0%
!! GATCCTTTGAAGTATCTACA+AGG - chr6_1:97150713-97150732 Msa0900910:intron 35.0%
TCACCTTGTAGATACTTCAA+AGG + chr6_1:97150719-97150738 None:intergenic 35.0%
! GGTGGTTTGAAATTGAGATG+CGG - chr6_1:97149908-97149927 Msa0900910:three_prime_UTR 40.0%
! GTGGTTTGAAATTGAGATGC+GGG - chr6_1:97149909-97149928 Msa0900910:three_prime_UTR 40.0%
!!! ATTATTTTAGACTCTCCGGG+TGG - chr6_1:97150175-97150194 Msa0900910:CDS 40.0%
GAACATATTTCGCGCTTACA+TGG - chr6_1:97150269-97150288 Msa0900910:CDS 40.0%
ATTAAGGCTCATCCAGACAT+TGG - chr6_1:97150308-97150327 Msa0900910:CDS 40.0%
ACGCTTAAAAATGCGACCAT+TGG + chr6_1:97150462-97150481 None:intergenic 40.0%
CTCTCTTCACAAACAATGTC+GGG + chr6_1:97150551-97150570 None:intergenic 40.0%
TGTTTGTGAAGAGAGAGATG+AGG - chr6_1:97150556-97150575 Msa0900910:CDS 40.0%
CAACTCTTCTTCATCAGATG+TGG + chr6_1:97150648-97150667 None:intergenic 40.0%
GATGAAGAAGAGTTGAGTGA+TGG - chr6_1:97150653-97150672 Msa0900910:CDS 40.0%
! GGTGGTTTGAAATTGAGATG+CGG - chr6_1:97149908-97149927 Msa0900910:three_prime_UTR 40.0%
! GTGGTTTGAAATTGAGATGC+GGG - chr6_1:97149909-97149928 Msa0900910:three_prime_UTR 40.0%
!!! ATTATTTTAGACTCTCCGGG+TGG - chr6_1:97150175-97150194 Msa0900910:CDS 40.0%
GAACATATTTCGCGCTTACA+TGG - chr6_1:97150269-97150288 Msa0900910:CDS 40.0%
ATTAAGGCTCATCCAGACAT+TGG - chr6_1:97150308-97150327 Msa0900910:CDS 40.0%
ACGCTTAAAAATGCGACCAT+TGG + chr6_1:97150462-97150481 None:intergenic 40.0%
CTCTCTTCACAAACAATGTC+GGG + chr6_1:97150551-97150570 None:intergenic 40.0%
TGTTTGTGAAGAGAGAGATG+AGG - chr6_1:97150556-97150575 Msa0900910:CDS 40.0%
CAACTCTTCTTCATCAGATG+TGG + chr6_1:97150648-97150667 None:intergenic 40.0%
GATGAAGAAGAGTTGAGTGA+TGG - chr6_1:97150653-97150672 Msa0900910:CDS 40.0%
! TGGATGTTTCTGATGAGGGA+AGG - chr6_1:97149887-97149906 Msa0900910:three_prime_UTR 45.0%
! ATGTTTCTGATGAGGGAAGG+TGG - chr6_1:97149890-97149909 Msa0900910:three_prime_UTR 45.0%
TTTCGCGCTTACATGGATTC+TGG - chr6_1:97150276-97150295 Msa0900910:CDS 45.0%
!!! AAGCGTTTTAACTTGCGCGA+CGG - chr6_1:97150476-97150495 Msa0900910:CDS 45.0%
TGAAGAGAGAGATGAGGATG+AGG - chr6_1:97150562-97150581 Msa0900910:CDS 45.0%
! AGGTGATGATGATGACACAG+AGG - chr6_1:97150733-97150752 Msa0900910:intron 45.0%
GACACAGAGGATGAAGTTGA+TGG - chr6_1:97150746-97150765 Msa0900910:intron 45.0%
! TGGATGTTTCTGATGAGGGA+AGG - chr6_1:97149887-97149906 Msa0900910:three_prime_UTR 45.0%
! ATGTTTCTGATGAGGGAAGG+TGG - chr6_1:97149890-97149909 Msa0900910:three_prime_UTR 45.0%
TTTCGCGCTTACATGGATTC+TGG - chr6_1:97150276-97150295 Msa0900910:CDS 45.0%
!!! AAGCGTTTTAACTTGCGCGA+CGG - chr6_1:97150476-97150495 Msa0900910:CDS 45.0%
TGAAGAGAGAGATGAGGATG+AGG - chr6_1:97150562-97150581 Msa0900910:CDS 45.0%
! AGGTGATGATGATGACACAG+AGG - chr6_1:97150733-97150752 Msa0900910:intron 45.0%
GACACAGAGGATGAAGTTGA+TGG - chr6_1:97150746-97150765 Msa0900910:intron 45.0%
CACGTTCAATCTTCTCCACC+CGG + chr6_1:97150193-97150212 None:intergenic 50.0%
TTCAACTGGCGACCAATGTC+TGG + chr6_1:97150323-97150342 None:intergenic 50.0%
AGAGAGAGATGAGGATGAGG+AGG - chr6_1:97150565-97150584 Msa0900910:CDS 50.0%
CACGTTCAATCTTCTCCACC+CGG + chr6_1:97150193-97150212 None:intergenic 50.0%
TTCAACTGGCGACCAATGTC+TGG + chr6_1:97150323-97150342 None:intergenic 50.0%
AGAGAGAGATGAGGATGAGG+AGG - chr6_1:97150565-97150584 Msa0900910:CDS 50.0%
GATGAGGATGAGGAGGACGA+CGG - chr6_1:97150572-97150591 Msa0900910:CDS 55.0%
GATGAGGATGAGGAGGACGA+CGG - chr6_1:97150572-97150591 Msa0900910:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 97149886 97151015 97149886 ID=Msa0900910;Name=Msa0900910
chr6_1 mRNA 97149886 97151015 97149886 ID=Msa0900910-mRNA-1;Parent=Msa0900910;Name=Msa0900910-mRNA-1;_AED=0.05;_eAED=0.13;_QI=0|0|0.5|1|1|1|2|243|222
chr6_1 exon 97149886 97150716 97149886 ID=Msa0900910-mRNA-1:exon:16969;Parent=Msa0900910-mRNA-1
chr6_1 exon 97150935 97151015 97150935 ID=Msa0900910-mRNA-1:exon:16968;Parent=Msa0900910-mRNA-1
chr6_1 CDS 97150935 97151015 97150935 ID=Msa0900910-mRNA-1:cds;Parent=Msa0900910-mRNA-1
chr6_1 CDS 97150129 97150716 97150129 ID=Msa0900910-mRNA-1:cds;Parent=Msa0900910-mRNA-1
chr6_1 three_prime_UTR 97149886 97150128 97149886 ID=Msa0900910-mRNA-1:three_prime_utr;Parent=Msa0900910-mRNA-1
Gene Sequence

>Msa0900910

ATGGATGTTTCTGATGAGGGAAGGTGGTTTGAAATTGAGATGCGGGAAAACCAAGAAAATATGATGAGGTACCGTATCCAAACTCTCCGGGTGGAGAAGATTGAACGTGCAAAAATAAATCAGAGTTTTGTTGACAAACTAAGCGATCACTGTCATAAGCGCAAAGAACATATTTCGCGCTTACATGGATTCTGGTTTAAGGCGATTAAGGCTCATCCAGACATTGGTCGCCAGTTGAATGCAATAGATTCAGAAATTTTTGAGAAACATCTCACTTCCATTGAAATTGTATATGAAAAAGATTCAGAATCAGGATTTATGATCACATTTGATTTCACTGAAAATGAATATTTTTCCAATGGTCGCATTTTTAAGCGTTTTAACTTGCGCGACGGAGATTTAATATCAACTGAATCTTCATGCATACATTGGATAAAAGATATTCCCGACATTGTTTGTGAAGAGAGAGATGAGGATGAGGAGGACGACGGTACATCTGAATCAAAACTTACCTCCCAAAGTTTTTTCAATTGGTTCACGTCCACATCTGATGAAGAAGAGTTGAGTGATGGAGATCATCTTGCAGATGCATTTGAGATGTTTTGGATTGATCCTTTGAAGTATCTACAAGGTGATGATGATGACACAGAGGATGAAGTTGATGGTTGA

Protein sequence

>Msa0900910

MDVSDEGRWFEIEMRENQENMMRYRIQTLRVEKIERAKINQSFVDKLSDHCHKRKEHISRLHGFWFKAIKAHPDIGRQLNAIDSEIFEKHLTSIEIVYEKDSESGFMITFDFTENEYFSNGRIFKRFNLRDGDLISTESSCIHWIKDIPDIVCEERDEDEEDDGTSESKLTSQSFFNWFTSTSDEEELSDGDHLADAFEMFWIDPLKYLQGDDDDTEDEVDG*