AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0681800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0681800 MtrunA17_Chr4g0027731 94.977 438 22 0 1 438 1 438 0.0 819
Msa0681800 MtrunA17_Chr2g0320571 65.487 452 139 4 1 438 1 449 0.0 554
Msa0681800 MtrunA17_Chr4g0028281 25.428 409 249 11 44 438 108 474 1.46e-30 123
Msa0681800 MtrunA17_Chr5g0447441 26.022 465 275 16 5 448 14 430 7.62e-30 121
Msa0681800 MtrunA17_Chr4g0044051 26.566 399 236 12 55 438 65 421 1.13e-29 120
Msa0681800 MtrunA17_Chr2g0327401 26.066 422 254 13 44 448 113 493 1.62e-27 115
Msa0681800 MtrunA17_Chr1g0171411 26.107 429 255 13 38 447 90 475 2.98e-27 114
Msa0681800 MtrunA17_Chr4g0028241 25.513 439 269 13 17 438 75 472 1.20e-26 112
Msa0681800 MtrunA17_Chr4g0028271 24.292 424 267 11 29 438 93 476 1.29e-25 109
Msa0681800 MtrunA17_Chr1g0199171 24.887 442 261 15 30 447 87 481 2.19e-24 105
Msa0681800 MtrunA17_Chr4g0028261 22.143 420 273 9 44 448 131 511 2.25e-24 105
Msa0681800 MtrunA17_Chr3g0118851 27.231 437 255 19 36 453 95 487 3.73e-24 105
Msa0681800 MtrunA17_Chr8g0385871 25.532 423 264 15 36 438 50 441 8.67e-24 103
Msa0681800 MtrunA17_Chr3g0131731 25.463 432 254 14 36 438 48 440 1.04e-22 100
Msa0681800 MtrunA17_Chr3g0118881 25.169 445 272 15 36 458 95 500 2.17e-22 99.8
Msa0681800 MtrunA17_Chr2g0281621 24.942 429 260 17 36 438 52 444 3.70e-22 99.0
Msa0681800 MtrunA17_Chr7g0249431 21.242 499 311 18 1 438 1 478 2.42e-21 96.7
Msa0681800 MtrunA17_Chr2g0327381 24.270 445 278 16 44 448 107 532 3.74e-21 96.3
Msa0681800 MtrunA17_Chr4g0047861 24.942 429 262 17 36 438 70 464 9.72e-21 94.7
Msa0681800 MtrunA17_Chr3g0118841 26.562 448 253 21 36 458 96 492 3.47e-20 93.2
Msa0681800 MtrunA17_Chr1g0153881 25.000 428 256 17 27 438 98 476 7.31e-20 92.0
Msa0681800 MtrunA17_Chr2g0327371 22.049 449 264 15 33 438 90 495 7.82e-19 89.0
Msa0681800 MtrunA17_Chr3g0118831 32.386 176 100 7 36 199 95 263 1.03e-18 88.6
Msa0681800 MtrunA17_Chr8g0338461 23.404 470 286 19 19 438 30 475 2.34e-18 87.4
Msa0681800 MtrunA17_Chr1g0165811 28.194 227 142 7 36 252 47 262 3.15e-18 84.3
Msa0681800 MtrunA17_Chr3g0094811 23.462 439 293 14 36 459 47 457 3.50e-18 86.7
Msa0681800 MtrunA17_Chr1g0153941 23.928 443 251 17 36 438 95 491 4.93e-18 86.7
Msa0681800 MtrunA17_Chr3g0118891 32.948 173 103 7 36 199 95 263 8.15e-18 85.5
Msa0681800 MtrunA17_Chr2g0304931 26.699 412 255 17 55 447 70 453 2.30e-17 84.3
Msa0681800 MtrunA17_Chr4g0038311 24.714 437 271 18 36 447 51 454 2.50e-17 84.3
Msa0681800 MtrunA17_Chr3g0090331 23.690 439 266 16 32 446 87 480 2.57e-17 84.3
Msa0681800 MtrunA17_Chr4g0068841 25.000 448 283 19 36 456 56 477 3.96e-17 83.6
Msa0681800 MtrunA17_Chr3g0118861 30.460 174 102 6 36 197 93 259 8.37e-17 82.4
Msa0681800 MtrunA17_Chr7g0221551 24.726 457 280 18 10 438 16 436 1.63e-16 81.6
Msa0681800 MtrunA17_Chr7g0234051 21.896 443 258 14 55 438 62 475 8.44e-16 79.7
Msa0681800 MtrunA17_Chr4g0020241 24.554 224 144 7 36 237 90 310 2.86e-15 77.8
Msa0681800 MtrunA17_Chr2g0309511 28.655 171 110 6 36 197 57 224 4.48e-15 77.4
Msa0681800 MtrunA17_Chr3g0126031 23.585 424 275 15 55 456 60 456 8.25e-15 76.3
Msa0681800 MtrunA17_Chr1g0199991 30.323 155 98 4 27 173 41 193 9.58e-15 76.3
Msa0681800 MtrunA17_Chr1g0153931 27.219 169 109 5 36 194 93 257 1.20e-14 73.9
Msa0681800 MtrunA17_Chr5g0431301 29.714 175 107 5 32 196 42 210 3.38e-14 74.7
Msa0681800 MtrunA17_Chr7g0219051 22.595 447 292 15 36 459 52 467 3.51e-14 74.3
Msa0681800 MtrunA17_Chr2g0309521 29.801 151 95 5 36 178 57 204 3.79e-14 74.3
Msa0681800 MtrunA17_Chr3g0126051 30.178 169 102 5 38 196 54 216 5.78e-14 73.9
Msa0681800 MtrunA17_Chr2g0309541 28.758 153 96 5 36 178 56 205 5.95e-14 73.9
Msa0681800 MtrunA17_Chr2g0281641 36.923 130 76 3 73 196 7 136 1.19e-13 72.4
Msa0681800 MtrunA17_Chr1g0199971 30.519 154 92 6 31 173 44 193 4.85e-13 70.9
Msa0681800 MtrunA17_Chr2g0318911 26.316 171 114 6 36 197 58 225 2.42e-12 68.9
Msa0681800 MtrunA17_Chr4g0068861 28.070 171 108 7 36 196 102 267 1.33e-11 66.6
Msa0681800 MtrunA17_Chr7g0234041 29.500 200 103 9 7 181 4 190 2.19e-11 65.9
Msa0681800 MtrunA17_Chr2g0285371 23.438 448 253 19 34 438 49 449 3.65e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0681800 AT2G27920.1 64.649 413 144 1 28 438 29 441 0.0 546
Msa0681800 AT2G27920.2 61.475 366 139 1 75 438 4 369 3.73e-156 448
Msa0681800 AT2G27920.3 58.410 327 134 1 114 438 48 374 4.68e-129 379
Msa0681800 AT3G45010.1 24.658 438 272 14 29 448 97 494 1.18e-29 121
Msa0681800 AT5G22980.1 26.066 422 252 12 44 448 111 489 2.11e-29 120
Msa0681800 AT1G15000.1 26.014 419 244 12 36 438 38 406 3.05e-29 119
Msa0681800 AT3G10410.1 25.455 440 260 16 30 447 96 489 1.44e-23 103
Msa0681800 AT3G52010.1 26.128 421 254 18 36 438 82 463 8.97e-22 98.2
Msa0681800 AT4G12910.1 22.222 468 292 15 19 438 34 477 1.49e-19 91.7
Msa0681800 AT2G35780.1 23.877 423 263 15 36 438 47 430 1.82e-18 87.8
Msa0681800 AT4G15100.1 25.576 434 277 17 36 446 61 471 1.90e-18 88.2
Msa0681800 AT3G25420.1 22.796 465 295 16 19 438 29 474 6.34e-18 86.7
Msa0681800 AT3G63470.1 23.544 412 273 14 36 438 98 476 1.06e-17 85.9
Msa0681800 AT1G11080.1 24.408 422 280 15 36 438 67 468 1.70e-17 85.1
Msa0681800 AT1G11080.2 24.408 422 280 15 36 438 67 468 2.02e-17 85.1
Msa0681800 AT2G33530.1 23.953 430 283 14 36 447 50 453 2.04e-17 84.7
Msa0681800 AT3G07990.1 24.356 427 274 16 28 438 43 436 2.86e-17 84.3
Msa0681800 AT4G12910.2 21.615 421 270 13 19 394 34 439 3.62e-17 84.0
Msa0681800 AT5G23210.3 28.821 229 145 8 36 252 68 290 9.26e-17 82.8
Msa0681800 AT5G23210.1 28.270 237 152 8 36 260 68 298 1.04e-16 82.8
Msa0681800 AT3G17180.2 25.746 268 165 10 36 285 56 307 1.23e-16 82.4
Msa0681800 AT3G17180.1 25.746 268 165 10 36 285 56 307 1.68e-16 82.0
Msa0681800 AT3G52020.1 24.886 438 261 20 36 448 96 490 1.70e-16 82.0
Msa0681800 AT1G43780.1 25.794 252 159 7 36 271 54 293 1.76e-16 82.0
Msa0681800 AT1G28110.1 24.286 420 271 15 36 438 48 437 1.84e-16 82.0
Msa0681800 AT1G28110.2 24.286 420 271 15 36 438 48 437 1.84e-16 82.0
Msa0681800 AT5G42240.1 26.800 250 147 8 36 264 49 283 5.55e-16 80.5
Msa0681800 AT2G24010.1 31.395 172 102 6 36 196 47 213 1.23e-15 79.3
Msa0681800 AT5G42230.1 32.000 150 94 3 55 196 60 209 1.86e-15 79.0
Msa0681800 AT5G42230.2 32.000 150 94 3 55 196 60 209 1.86e-15 79.0
Msa0681800 AT4G30810.1 23.855 415 275 17 46 447 67 453 5.87e-15 77.4
Msa0681800 AT2G05850.1 23.185 427 260 17 36 438 81 463 6.23e-15 77.4
Msa0681800 AT2G24000.1 30.994 171 104 6 36 196 47 213 1.32e-14 76.3
Msa0681800 AT5G08260.1 30.612 147 89 4 36 173 56 198 1.93e-14 75.9
Msa0681800 AT1G61130.1 34.043 141 84 3 65 196 75 215 2.57e-14 75.5
Msa0681800 AT3G02110.1 30.935 139 90 2 65 197 81 219 4.38e-14 74.7
Msa0681800 AT2G12480.2 33.333 126 77 2 55 173 63 188 4.92e-14 74.3
Msa0681800 AT2G12480.4 33.333 126 77 2 55 173 63 188 5.07e-14 74.3
Msa0681800 AT2G12480.1 33.333 126 77 2 55 173 63 188 5.36e-14 74.3
Msa0681800 AT2G12480.3 33.333 126 77 2 55 173 63 188 6.00e-14 74.3
Msa0681800 AT4G30610.1 30.978 184 97 8 32 197 45 216 2.03e-13 72.4
Msa0681800 AT2G35770.1 29.609 179 111 6 28 196 47 220 3.26e-13 72.0
Msa0681800 AT1G73290.2 29.655 145 85 4 34 166 47 186 9.86e-13 69.3
Msa0681800 AT1G73290.3 29.655 145 85 4 34 166 47 186 2.65e-12 68.9
Msa0681800 AT1G73300.1 30.345 145 84 4 34 166 48 187 2.81e-12 68.9
Msa0681800 AT1G73300.2 30.345 145 84 4 34 166 48 187 2.81e-12 68.9
Msa0681800 AT5G22960.1 27.660 188 106 6 5 187 8 170 3.08e-12 65.9
Msa0681800 AT5G36180.1 29.814 161 95 5 19 166 32 187 3.55e-12 68.6
Msa0681800 AT1G73290.1 29.655 145 85 4 34 166 47 186 3.64e-12 68.6
Msa0681800 AT1G73280.2 29.444 180 99 6 10 166 13 187 7.11e-12 67.4
Msa0681800 AT1G73280.1 29.444 180 99 6 10 166 13 187 1.03e-11 67.0
Msa0681800 AT3G56540.1 27.848 158 103 5 19 169 79 232 1.18e-11 65.5
Msa0681800 AT1G73270.2 31.034 145 83 4 34 166 48 187 1.19e-11 67.0
Msa0681800 AT1G73270.1 31.034 145 83 4 34 166 48 187 1.21e-11 67.0
Msa0681800 AT1G73310.2 29.655 145 85 4 34 166 48 187 6.03e-11 64.7
Msa0681800 AT1G73310.1 29.655 145 85 4 34 166 48 187 7.87e-11 64.3

Find 117 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACAATTCTATTGGATTCT+TGG 0.200204 4_4:-42487203 Msa0681800:CDS
CCCTGCAACCAGAGAATTAT+TGG 0.209686 4_4:+42490593 None:intergenic
CTTCTTACCTCATCTATTCT+TGG 0.218674 4_4:+42488100 None:intergenic
CCTTAGGGAAGTAAGATATT+TGG 0.230572 4_4:+42488354 None:intergenic
AAACTTGGCGCTTTACTAAA+TGG 0.231890 4_4:-42488301 Msa0681800:CDS
GGCAAATTTGCTGTCACTCT+TGG 0.293076 4_4:-42489697 Msa0681800:CDS
GCCAATAATTCTCTGGTTGC+AGG 0.294647 4_4:-42490594 Msa0681800:CDS
CAAGAATCCAATAGAATTGT+AGG 0.295802 4_4:+42487204 None:intergenic
GTCATTGGATAGGGTGCTTC+AGG 0.303141 4_4:-42490369 Msa0681800:intron
TACAATTTCTTGGTCGATTC+GGG 0.308824 4_4:-42488442 Msa0681800:CDS
TTGCAGAAGCACACATGTTT+TGG 0.324024 4_4:-42490664 Msa0681800:intron
TAGACTTACGTGACATTCTC+AGG 0.332673 4_4:+42488250 None:intergenic
AAGATAAGTGAAAACTTCAT+TGG 0.346770 4_4:+42488141 None:intergenic
TTACAATTTCTTGGTCGATT+CGG 0.359915 4_4:-42488443 Msa0681800:CDS
TTACTTACAACAAAATCTTC+TGG 0.366194 4_4:+42489206 None:intergenic
TACATTGTGGCAGAGTCTTA+TGG 0.367175 4_4:-42489721 Msa0681800:CDS
CCCATTCTTCTGATCCATCT+TGG 0.371873 4_4:+42490827 None:intergenic
CAAAAGAATGAAAAGGGTAT+TGG 0.372196 4_4:+42490980 None:intergenic
ACTTATGTAGGTGTGGCATT+AGG 0.381627 4_4:-42489245 Msa0681800:intron
GGATAGGGTGCTTCAGGTGT+TGG 0.387749 4_4:-42490363 Msa0681800:intron
CGCTCATTAAAGATGATGCT+TGG 0.395330 4_4:-42486034 Msa0681800:CDS
AGAAGAAGACCTTGTCCTTA+GGG 0.396772 4_4:+42488339 None:intergenic
GATAGGGTGCTTCAGGTGTT+GGG 0.400100 4_4:-42490362 Msa0681800:intron
GAGAAGAAGACCTTGTCCTT+AGG 0.404625 4_4:+42488338 None:intergenic
GTCTCAAGACTTGATGATAA+TGG 0.408231 4_4:-42488964 Msa0681800:CDS
GAAGAATCTTCCACTCTGTA+TGG 0.410067 4_4:+42490626 None:intergenic
AACAACCATGTTGAATTCCT+TGG 0.412636 4_4:+42490294 None:intergenic
CCAAGATGGATCAGAAGAAT+GGG 0.416640 4_4:-42490827 Msa0681800:CDS
TTCTTGGTCGATTCGGGAAG+TGG 0.417221 4_4:-42488436 Msa0681800:CDS
GAGAAGCAAAAGAATGAAAA+GGG 0.421945 4_4:+42490974 None:intergenic
AGAGAAGCAAAAGAATGAAA+AGG 0.421991 4_4:+42490973 None:intergenic
AACCCAAGCTTCAGTCCCTT+TGG 0.424615 4_4:+42487643 None:intergenic
GCTCATTAAAGATGATGCTT+GGG 0.440255 4_4:-42486033 Msa0681800:CDS
GATAGTAACATTGGCTCCCT+TGG 0.440557 4_4:+42487946 None:intergenic
AAACTGTATCCTGTTCCAAC+TGG 0.449212 4_4:+42489856 None:intergenic
CTACACTAAGTTAGCGCCCT+AGG 0.449298 4_4:+42485999 None:intergenic
TTCTATTGGATTCTTGGAGC+TGG 0.449461 4_4:-42487197 Msa0681800:intron
GGTGCTTCAGGTGTTGGGAT+TGG 0.451236 4_4:-42490357 Msa0681800:CDS
AAGAGAAGAATGATGGGAAA+AGG 0.459345 4_4:+42490895 None:intergenic
TCTTTATTCCGTAGGTGGGA+AGG 0.462971 4_4:-42487314 Msa0681800:intron
AAGGGACTGAAGCTTGGGTT+AGG 0.462980 4_4:-42487640 Msa0681800:CDS
ACCAAGATGGATCAGAAGAA+TGG 0.467901 4_4:-42490828 Msa0681800:CDS
TACCAAAGGGACTGAAGCTT+GGG 0.469620 4_4:-42487645 Msa0681800:CDS
CTACCAAAGGGACTGAAGCT+TGG 0.476951 4_4:-42487646 Msa0681800:CDS
CAGGACAATCCAGTTGGAAC+AGG 0.483965 4_4:-42489865 Msa0681800:CDS
TCTGAATTTGCAGTTGTCAT+GGG 0.484342 4_4:-42489004 Msa0681800:intron
ATCTAATTTGTGCTACCAAA+GGG 0.486363 4_4:-42487658 Msa0681800:CDS
TGAAAGGAAGAGAAGAATGA+TGG 0.491304 4_4:+42490888 None:intergenic
CTTTATTCCGTAGGTGGGAA+GGG 0.493124 4_4:-42487313 Msa0681800:intron
GATTAATAAAAGAAAGATCA+AGG 0.496246 4_4:-42488387 Msa0681800:CDS
CAAGTCTTGAGACGTCTTTG+AGG 0.497604 4_4:+42488974 None:intergenic
AGGACAAGGTCTTCTTCTCC+TGG 0.502448 4_4:-42488334 Msa0681800:CDS
ATCTGAATTTGCAGTTGTCA+TGG 0.503567 4_4:-42489005 Msa0681800:intron
AGCCATGGCCAATAATTCTC+TGG 0.505294 4_4:-42490601 Msa0681800:CDS
TGAAAATTGACGCAATGATA+CGG 0.509825 4_4:+42491064 None:intergenic
AATTCAACATGGTTGTTGAA+AGG 0.521568 4_4:-42490288 Msa0681800:CDS
TAAAAGAAAGATCAAGGAAG+AGG 0.522688 4_4:-42488381 Msa0681800:CDS
TCATATCCTCTGTCCTTGAC+TGG 0.522768 4_4:-42491111 Msa0681800:five_prime_UTR
GATCATGCTACTCCACCTGC+TGG 0.523388 4_4:-42486065 Msa0681800:CDS
GAAAGGAGATCTCTTGTTTG+TGG 0.524255 4_4:-42490271 Msa0681800:intron
TTAATGATGCAACTGACTCA+TGG 0.525154 4_4:-42488789 Msa0681800:CDS
AACCAGAGAATTATTGGCCA+TGG 0.526681 4_4:+42490599 None:intergenic
TGGATACCAGCTTGAAGCCA+AGG 0.530260 4_4:-42490311 Msa0681800:CDS
TGAATTCCTTGGCTTCAAGC+TGG 0.530298 4_4:+42490305 None:intergenic
AAATTGATACTTACACTTGG+AGG 0.534832 4_4:-42489643 Msa0681800:intron
TCCATTGTAGATAGTAACAT+TGG 0.536914 4_4:+42487937 None:intergenic
ATCAAGAAGCAACTTGAAGA+TGG 0.537399 4_4:-42488817 Msa0681800:CDS
CCTGGTGGTGATGATAAACT+TGG 0.538460 4_4:-42488316 Msa0681800:CDS
CCAAATATCTTACTTCCCTA+AGG 0.539917 4_4:-42488354 Msa0681800:CDS
GAAAGGAAGAGAAGAATGAT+GGG 0.542790 4_4:+42490889 None:intergenic
GATCTAATTTGTGCTACCAA+AGG 0.546200 4_4:-42487659 Msa0681800:CDS
TACACTAAGTTAGCGCCCTA+GGG 0.555544 4_4:+42486000 None:intergenic
TTTGTTACCACCACAGTAGA+GGG 0.555703 4_4:+42487259 None:intergenic
AAGACTTGATGATAATGGAC+TGG 0.560259 4_4:-42488959 Msa0681800:CDS
AAAGAGTCCTTTGTACATTG+TGG 0.565413 4_4:-42489734 Msa0681800:CDS
GACATATCAGCAATGGCAGT+AGG 0.570461 4_4:-42488409 Msa0681800:CDS
CCAATAATTCTCTGGTTGCA+GGG 0.571892 4_4:-42490593 Msa0681800:CDS
GATATATACGCTGCCAGTCA+AGG 0.574900 4_4:+42491098 None:intergenic
CTCATTAAAGATGATGCTTG+GGG 0.583566 4_4:-42486032 Msa0681800:CDS
CTGAATTTGCAGTTGTCATG+GGG 0.584944 4_4:-42489003 Msa0681800:intron
TGAAATGATTATAGATGGGG+AGG 0.588913 4_4:-42488169 Msa0681800:intron
ATGAGCGGCAACAATCCAGC+AGG 0.591371 4_4:+42486050 None:intergenic
TGGAAGATTCTTCAAAGCCA+TGG 0.593562 4_4:-42490616 Msa0681800:CDS
GGCTTCAAGCTGGTATCCAA+AGG 0.594499 4_4:+42490315 None:intergenic
ATTTGTTACCACCACAGTAG+AGG 0.594837 4_4:+42487258 None:intergenic
AAGAAATTGATACTTACACT+TGG 0.595456 4_4:-42489646 Msa0681800:CDS
GCCAATGTTACTATCTACAA+TGG 0.596962 4_4:-42487938 Msa0681800:CDS
GTGTGGCATTAGGAGACAGT+TGG 0.599447 4_4:-42489235 Msa0681800:CDS
ATTGACGCAATGATACGGAT+AGG 0.601072 4_4:+42491069 None:intergenic
AGACTTACGTGACATTCTCA+GGG 0.602881 4_4:+42488251 None:intergenic
GGTTGATCAGCTACTGACCA+AGG 0.605656 4_4:-42487963 Msa0681800:CDS
CTTGAGACGTCTTTGAGGAG+AGG 0.606726 4_4:+42488979 None:intergenic
TATGAAGCCAAGAATAGATG+AGG 0.609873 4_4:-42488107 Msa0681800:intron
AGCGGCAACAATCCAGCAGG+TGG 0.611685 4_4:+42486053 None:intergenic
ATAACACAGGACAATCCAGT+TGG 0.618495 4_4:-42489871 Msa0681800:intron
GACTCTGCCACAATGTACAA+AGG 0.618531 4_4:+42489727 None:intergenic
TGGTTCAGACATATCAGCAA+TGG 0.623686 4_4:-42488416 Msa0681800:CDS
ATCTTACTTCCCTAAGGACA+AGG 0.628942 4_4:-42488348 Msa0681800:CDS
CCAAGTTTATCATCACCACC+AGG 0.637624 4_4:+42488316 None:intergenic
TTCAATAAGCAGCAACAATG+TGG 0.638580 4_4:-42488746 Msa0681800:intron
CAACAATGTTGTCAAATCTG+CGG 0.648619 4_4:+42489789 None:intergenic
CAAGCATCATCTTTAATGAG+CGG 0.653891 4_4:+42486035 None:intergenic
TCATTAAAGATGATGCTTGG+GGG 0.654702 4_4:-42486031 Msa0681800:CDS
TGAAGCCAAGGAATTCAACA+TGG 0.659940 4_4:-42490299 Msa0681800:CDS
ACAAGGTCTTCTTCTCCTGG+TGG 0.673235 4_4:-42488331 Msa0681800:CDS
GGTGGTAACAAATCGACCAA+AGG 0.673311 4_4:-42487248 Msa0681800:CDS
GACAGAACACCCCTCTACTG+TGG 0.677504 4_4:-42487269 Msa0681800:CDS
AGAACACCCCTCTACTGTGG+TGG 0.678028 4_4:-42487266 Msa0681800:CDS
TTGTTACCACCACAGTAGAG+GGG 0.680201 4_4:+42487260 None:intergenic
CAAGATGGATCAGAAGAATG+GGG 0.681105 4_4:-42490826 Msa0681800:CDS
GTTGATCAGCTACTGACCAA+GGG 0.682526 4_4:-42487962 Msa0681800:CDS
ATAATTCTCTGGTTGCAGGG+TGG 0.685909 4_4:-42490590 Msa0681800:CDS
TGGAGATCAGTATAGCTCAG+AGG 0.690094 4_4:-42488840 Msa0681800:intron
GAAATGATTATAGATGGGGA+GGG 0.690725 4_4:-42488168 Msa0681800:intron
TTATAAAAGTCCATACAGAG+TGG 0.699820 4_4:-42490636 Msa0681800:CDS
ATTGTGGCAGAGTCTTATGG+TGG 0.700649 4_4:-42489718 Msa0681800:CDS
ACGCTGCCAGTCAAGGACAG+AGG 0.702856 4_4:+42491105 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATAGTTTATACAAAA+GGG + chr4_4:42487841-42487860 None:intergenic 10.0%
!!! ATTTTTTTTATTTTTTTTCG+AGG - chr4_4:42489097-42489116 Msa0681800:intron 10.0%
!!! TTTTTTTTATTTTTTTTCGA+GGG - chr4_4:42489098-42489117 Msa0681800:intron 10.0%
!!! TTTTATTTTAATTATAAGCT+TGG - chr4_4:42489124-42489143 Msa0681800:intron 10.0%
!! TTTAAACTTGTATATTTATA+TGG + chr4_4:42490094-42490113 None:intergenic 10.0%
!!! TATTTGAATTTTAGTTTTTA+AGG - chr4_4:42490159-42490178 Msa0681800:intron 10.0%
!! ACTTATATGAATTAAATACA+AGG + chr4_4:42486987-42487006 None:intergenic 15.0%
!!! ATCAAATATTTTTAGTGATT+TGG + chr4_4:42487053-42487072 None:intergenic 15.0%
!! CTAAAAATAGTTTATACAAA+AGG + chr4_4:42487842-42487861 None:intergenic 15.0%
!!! TTAAAGAAAAAGTATGATTT+TGG - chr4_4:42487873-42487892 Msa0681800:intron 15.0%
!!! GAATTTTCTCAAATTATTAA+TGG - chr4_4:42488593-42488612 Msa0681800:intron 15.0%
!!! CTTAAATTTTTTGTTGAAAT+AGG - chr4_4:42488680-42488699 Msa0681800:intron 15.0%
!!! TTAATAATTTTAACCTTTAC+AGG - chr4_4:42489166-42489185 Msa0681800:intron 15.0%
!! TAGTTCAATAAGTTTAATTA+CGG + chr4_4:42489723-42489742 None:intergenic 15.0%
!! CTAGTTACTATTAATAAATT+TGG - chr4_4:42490222-42490241 Msa0681800:intron 15.0%
!! TATTAATAAATTTGGAAATG+TGG - chr4_4:42490230-42490249 Msa0681800:intron 15.0%
!!! TTTCCAAATTCTATATTTTA+CGG - chr4_4:42490353-42490372 Msa0681800:CDS 15.0%
!!! AGATAAAATTCTCTTATTTT+TGG - chr4_4:42490520-42490539 Msa0681800:intron 15.0%
!!! TTTTAGATATAAAAATCAGA+AGG - chr4_4:42490957-42490976 Msa0681800:five_prime_UTR 15.0%
!! ATTTATGATGAGTATAAATT+GGG - chr4_4:42491037-42491056 Msa0681800:five_prime_UTR 15.0%
!! CATTGATATCGATAATAATA+TGG - chr4_4:42486608-42486627 Msa0681800:intron 20.0%
!! TCATACATAAACACATAAAA+TGG + chr4_4:42486745-42486764 None:intergenic 20.0%
!! TAAAATAACTATGACGATTA+CGG + chr4_4:42487132-42487151 None:intergenic 20.0%
!! TGAAAGAGTAAAACTATTTA+TGG - chr4_4:42487182-42487201 Msa0681800:intron 20.0%
!!! ATTGGTCTTATTTCAATTTA+TGG - chr4_4:42487577-42487596 Msa0681800:intron 20.0%
!! ATATAATTCAGTGTAATCAT+AGG - chr4_4:42487686-42487705 Msa0681800:intron 20.0%
!! ATAAATTCTGACTAATGAAT+GGG - chr4_4:42487771-42487790 Msa0681800:intron 20.0%
!! GTTCAACAAAATAAACTTAT+TGG + chr4_4:42487983-42488002 None:intergenic 20.0%
!! AAAATATAGAGCAAAATGTA+TGG - chr4_4:42488256-42488275 Msa0681800:intron 20.0%
!! TATTAATGGTGTTTAAGTAT+CGG - chr4_4:42488607-42488626 Msa0681800:intron 20.0%
!!! ATAGGATTTTTACAATTTCT+TGG - chr4_4:42488698-42488717 Msa0681800:intron 20.0%
!! GATTAATAAAAGAAAGATCA+AGG - chr4_4:42488763-42488782 Msa0681800:CDS 20.0%
!! TTAGTTTCGATAATAATAAG+AGG - chr4_4:42489262-42489281 Msa0681800:intron 20.0%
!! GAAAATGAATAAGATACTAT+TGG - chr4_4:42489284-42489303 Msa0681800:intron 20.0%
!!! TTTAATGTTTTTTTTTCCGA+AGG + chr4_4:42489449-42489468 None:intergenic 20.0%
!! AAAGTGTGTTTGATTATTAT+AGG - chr4_4:42489466-42489485 Msa0681800:intron 20.0%
!! AAAGCCAATGTTATAAAAAT+CGG - chr4_4:42489593-42489612 Msa0681800:intron 20.0%
!! AGTTTAATTACGGTTTAATT+TGG + chr4_4:42489713-42489732 None:intergenic 20.0%
!!! ATTCTTATCTTCTTTTTTCA+CGG - chr4_4:42490128-42490147 Msa0681800:intron 20.0%
!! AAACCGTAAAATATAGAATT+TGG + chr4_4:42490359-42490378 None:intergenic 20.0%
!!! GTAGTACTATAATTTTAATG+TGG + chr4_4:42490573-42490592 None:intergenic 20.0%
!!! GTATTTTCTTCAAAATACTT+AGG - chr4_4:42490634-42490653 Msa0681800:CDS 20.0%
!! TGAAAATAATGCAAGAAAAA+AGG - chr4_4:42490983-42491002 Msa0681800:five_prime_UTR 20.0%
!! GAAAATAATGCAAGAAAAAA+GGG - chr4_4:42490984-42491003 Msa0681800:five_prime_UTR 20.0%
!! CATTTATGATGAGTATAAAT+TGG - chr4_4:42491036-42491055 Msa0681800:five_prime_UTR 20.0%
!!! GAAGTTTTTCCATTTTTTGA+AGG + chr4_4:42486233-42486252 None:intergenic 25.0%
! GTAGAAGAAAAAATGATGAA+AGG + chr4_4:42486281-42486300 None:intergenic 25.0%
! ATAAACACAAAAGACTTACT+AGG + chr4_4:42486366-42486385 None:intergenic 25.0%
!! TAGTAAGTCTTTTGTGTTTA+TGG - chr4_4:42486365-42486384 Msa0681800:intron 25.0%
!! TCGATAATAATATGGTGTTT+TGG - chr4_4:42486616-42486635 Msa0681800:intron 25.0%
! CATATAAGTTGTAAGTGTAA+AGG - chr4_4:42486998-42487017 Msa0681800:intron 25.0%
!!! AAATCTTGTTTTTGACTATG+TGG - chr4_4:42487224-42487243 Msa0681800:CDS 25.0%
! AAGAAATTGATACTTACACT+TGG - chr4_4:42487504-42487523 Msa0681800:intron 25.0%
! CATAAATTCTGACTAATGAA+TGG - chr4_4:42487770-42487789 Msa0681800:intron 25.0%
!! TGGTAAACTTTTACTTATGT+AGG - chr4_4:42487893-42487912 Msa0681800:intron 25.0%
! TTACTTACAACAAAATCTTC+TGG + chr4_4:42487947-42487966 None:intergenic 25.0%
!!! AAGTTTATTTTGTTGAACAG+TGG - chr4_4:42487985-42488004 Msa0681800:intron 25.0%
!! TATTTTCTACTTGAGAAGAT+GGG + chr4_4:42488242-42488261 None:intergenic 25.0%
!! ATATTTTCTACTTGAGAAGA+TGG + chr4_4:42488243-42488262 None:intergenic 25.0%
!! TTTATCTAACAAACCCATTT+TGG - chr4_4:42488290-42488309 Msa0681800:CDS 25.0%
! ACAAAAGAGAACTCATTTAT+GGG + chr4_4:42488455-42488474 None:intergenic 25.0%
! TACAAAAGAGAACTCATTTA+TGG + chr4_4:42488456-42488475 None:intergenic 25.0%
! TGGACTTAATATATACATTG+CGG + chr4_4:42488484-42488503 None:intergenic 25.0%
!! TGAACAAAACTTTTCATTCA+TGG + chr4_4:42488938-42488957 None:intergenic 25.0%
! ATCCATGAAATGATTATAGA+TGG - chr4_4:42488976-42488995 Msa0681800:CDS 25.0%
! TCCATGAAATGATTATAGAT+GGG - chr4_4:42488977-42488996 Msa0681800:CDS 25.0%
! AAGATAAGTGAAAACTTCAT+TGG + chr4_4:42489012-42489031 None:intergenic 25.0%
!!! TTTTTATAACATTGGCTTTG+AGG + chr4_4:42489592-42489611 None:intergenic 25.0%
!! TGATCCGATTTTTATAACAT+TGG + chr4_4:42489600-42489619 None:intergenic 25.0%
! ACAAAAACCTACAATTCTAT+TGG - chr4_4:42489939-42489958 Msa0681800:intron 25.0%
!!! ATAGTTGTTTTAGTGATGAT+TGG + chr4_4:42489997-42490016 None:intergenic 25.0%
!! ACATTAAGTTTTTGTGACAT+TGG - chr4_4:42490659-42490678 Msa0681800:CDS 25.0%
TGAAAATTGACGCAATGATA+CGG + chr4_4:42486089-42486108 None:intergenic 30.0%
! CAAAAGAATGAAAAGGGTAT+TGG + chr4_4:42486173-42486192 None:intergenic 30.0%
GAGAAGCAAAAGAATGAAAA+GGG + chr4_4:42486179-42486198 None:intergenic 30.0%
AGAGAAGCAAAAGAATGAAA+AGG + chr4_4:42486180-42486199 None:intergenic 30.0%
CTCTTCTTTCCTTCAAAAAA+TGG - chr4_4:42486221-42486240 Msa0681800:intron 30.0%
TTATAAAAGTCCATACAGAG+TGG - chr4_4:42486514-42486533 Msa0681800:intron 30.0%
! GAATGAGATTTTCATGTCAT+TGG - chr4_4:42486766-42486785 Msa0681800:intron 30.0%
! AGATTTTCATGTCATTGGAT+AGG - chr4_4:42486771-42486790 Msa0681800:intron 30.0%
! GATTTTCATGTCATTGGATA+GGG - chr4_4:42486772-42486791 Msa0681800:intron 30.0%
!! ATTGGAAATTTTGAAGAGGT+TGG - chr4_4:42486811-42486830 Msa0681800:intron 30.0%
AATTCAACATGGTTGTTGAA+AGG - chr4_4:42486862-42486881 Msa0681800:intron 30.0%
!!! AAAAGCACTTTTCATGATGA+TGG + chr4_4:42486928-42486947 None:intergenic 30.0%
TTGATGTCATATGATAACAC+AGG - chr4_4:42487266-42487285 Msa0681800:CDS 30.0%
AAATTGATACTTACACTTGG+AGG - chr4_4:42487507-42487526 Msa0681800:intron 30.0%
!! TAACTTGTTCTTCTTTGTGT+TGG - chr4_4:42487800-42487819 Msa0681800:intron 30.0%
! AACTTTTACTTATGTAGGTG+TGG - chr4_4:42487898-42487917 Msa0681800:intron 30.0%
!!! GTGGTTTTAAATTGCAATTG+TGG - chr4_4:42488004-42488023 Msa0681800:intron 30.0%
! TGCATTTTACCGCAATATAA+AGG + chr4_4:42488042-42488061 None:intergenic 30.0%
CTGTAATTGTAGTTACAATG+CGG - chr4_4:42488073-42488092 Msa0681800:intron 30.0%
ACTTTAGATCAAAGTGTATC+TGG - chr4_4:42488524-42488543 Msa0681800:intron 30.0%
TTACAATTTCTTGGTCGATT+CGG - chr4_4:42488707-42488726 Msa0681800:intron 30.0%
TAAAAGAAAGATCAAGGAAG+AGG - chr4_4:42488769-42488788 Msa0681800:CDS 30.0%
CCATGAAATGATTATAGATG+GGG - chr4_4:42488978-42488997 Msa0681800:CDS 30.0%
! GAAGTTTTCACTTATCTTGA+AGG - chr4_4:42489014-42489033 Msa0681800:intron 30.0%
TCCATTGTAGATAGTAACAT+TGG + chr4_4:42489216-42489235 None:intergenic 30.0%
! CATTAAAGTGTGTTTTCCTT+CGG - chr4_4:42489430-42489449 Msa0681800:intron 30.0%
! ATCTAATTTGTGCTACCAAA+GGG - chr4_4:42489492-42489511 Msa0681800:intron 30.0%
ATCTTCTTTCTTTATTCCGT+AGG - chr4_4:42489828-42489847 Msa0681800:CDS 30.0%
GTATGACTTGAAAAATCCTT+TGG + chr4_4:42489921-42489940 None:intergenic 30.0%
CAAGAATCCAATAGAATTGT+AGG + chr4_4:42489949-42489968 None:intergenic 30.0%
CTACAATTCTATTGGATTCT+TGG - chr4_4:42489947-42489966 Msa0681800:intron 30.0%
! CTTTTAACAGCTCATTCATA+TGG - chr4_4:42490315-42490334 Msa0681800:CDS 30.0%
AAAATTCTATGTGAGCAAGA+AGG - chr4_4:42490396-42490415 Msa0681800:intron 30.0%
AAATTCTATGTGAGCAAGAA+GGG - chr4_4:42490397-42490416 Msa0681800:intron 30.0%
GTAATCTCAAATATCGATGT+CGG - chr4_4:42490877-42490896 Msa0681800:CDS 30.0%
! AAAAAAAGCTTTTACACGTC+TGG + chr4_4:42490932-42490951 None:intergenic 30.0%
! AAGAGAAGAATGATGGGAAA+AGG + chr4_4:42486258-42486277 None:intergenic 35.0%
GAAAGGAAGAGAAGAATGAT+GGG + chr4_4:42486264-42486283 None:intergenic 35.0%
TGAAAGGAAGAGAAGAATGA+TGG + chr4_4:42486265-42486284 None:intergenic 35.0%
ACAGCTTTCAAAAACCAAGA+TGG - chr4_4:42486309-42486328 Msa0681800:intron 35.0%
CCAATCAAGTAGTACTTACA+GGG + chr4_4:42486586-42486605 None:intergenic 35.0%
ACCAATCAAGTAGTACTTAC+AGG + chr4_4:42486587-42486606 None:intergenic 35.0%
!! TGGGATTGGAAATTTTGAAG+AGG - chr4_4:42486807-42486826 Msa0681800:intron 35.0%
AACAACCATGTTGAATTCCT+TGG + chr4_4:42486859-42486878 None:intergenic 35.0%
! GATCTCTTGTTTGTGGTAAT+TGG - chr4_4:42486886-42486905 Msa0681800:intron 35.0%
CAACAATGTTGTCAAATCTG+CGG + chr4_4:42487364-42487383 None:intergenic 35.0%
AAAGAGTCCTTTGTACATTG+TGG - chr4_4:42487416-42487435 Msa0681800:intron 35.0%
! TGAGATCTGAAATCTGAAGA+TGG + chr4_4:42487556-42487575 None:intergenic 35.0%
TCAGATTTCAGATCTCAGAT+TGG - chr4_4:42487559-42487578 Msa0681800:intron 35.0%
! TGTAATCATAGGTGTTGTGT+CGG - chr4_4:42487697-42487716 Msa0681800:intron 35.0%
!! TGTTCTTCTTTGTGTTGGTT+AGG - chr4_4:42487805-42487824 Msa0681800:intron 35.0%
!! TTGTGTTGGTTAGGAAATGA+TGG - chr4_4:42487814-42487833 Msa0681800:intron 35.0%
ATCTGAATTTGCAGTTGTCA+TGG - chr4_4:42488145-42488164 Msa0681800:CDS 35.0%
TCTGAATTTGCAGTTGTCAT+GGG - chr4_4:42488146-42488165 Msa0681800:CDS 35.0%
! GTCTCAAGACTTGATGATAA+TGG - chr4_4:42488186-42488205 Msa0681800:intron 35.0%
!! AAGACTTGATGATAATGGAC+TGG - chr4_4:42488191-42488210 Msa0681800:intron 35.0%
!! ATGGACTGGAAAAATCAAAC+AGG - chr4_4:42488205-42488224 Msa0681800:intron 35.0%
GCTATACTGATCTCCAAAAT+GGG + chr4_4:42488306-42488325 None:intergenic 35.0%
AGCTATACTGATCTCCAAAA+TGG + chr4_4:42488307-42488326 None:intergenic 35.0%
ATCAAGAAGCAACTTGAAGA+TGG - chr4_4:42488333-42488352 Msa0681800:CDS 35.0%
! TTAATGATGCAACTGACTCA+TGG - chr4_4:42488361-42488380 Msa0681800:CDS 35.0%
TTCAATAAGCAGCAACAATG+TGG - chr4_4:42488404-42488423 Msa0681800:CDS 35.0%
! TACACTTTGATCTAAAGTGC+TGG + chr4_4:42488522-42488541 None:intergenic 35.0%
ATTTCTGTGTTGTGTTGTGT+CGG + chr4_4:42488553-42488572 None:intergenic 35.0%
! TACAATTTCTTGGTCGATTC+GGG - chr4_4:42488708-42488727 Msa0681800:intron 35.0%
CCTTAGGGAAGTAAGATATT+TGG + chr4_4:42488799-42488818 None:intergenic 35.0%
CCAAATATCTTACTTCCCTA+AGG - chr4_4:42488796-42488815 Msa0681800:CDS 35.0%
AAACTTGGCGCTTTACTAAA+TGG - chr4_4:42488849-42488868 Msa0681800:intron 35.0%
CCCCATCTATAATCATTTCA+TGG + chr4_4:42488981-42489000 None:intergenic 35.0%
TGAAATGATTATAGATGGGG+AGG - chr4_4:42488981-42489000 Msa0681800:CDS 35.0%
GAAATGATTATAGATGGGGA+GGG - chr4_4:42488982-42489001 Msa0681800:CDS 35.0%
TATGAAGCCAAGAATAGATG+AGG - chr4_4:42489043-42489062 Msa0681800:intron 35.0%
CTTCTTACCTCATCTATTCT+TGG + chr4_4:42489053-42489072 None:intergenic 35.0%
GCCAATGTTACTATCTACAA+TGG - chr4_4:42489212-42489231 Msa0681800:intron 35.0%
! TACTATTGGACATGTTGACT+TGG - chr4_4:42489298-42489317 Msa0681800:intron 35.0%
! GATCTAATTTGTGCTACCAA+AGG - chr4_4:42489491-42489510 Msa0681800:intron 35.0%
AAAAATCGGATCAGACTAGT+CGG - chr4_4:42489607-42489626 Msa0681800:intron 35.0%
!! CATTGATTCGATATTCGATC+CGG - chr4_4:42489762-42489781 Msa0681800:CDS 35.0%
TTCTTTCTTTATTCCGTAGG+TGG - chr4_4:42489831-42489850 Msa0681800:CDS 35.0%
TCTTTCTTTATTCCGTAGGT+GGG - chr4_4:42489832-42489851 Msa0681800:CDS 35.0%
GAAGGGCTAAAAAGTTTCTT+GGG - chr4_4:42489854-42489873 Msa0681800:CDS 35.0%
AAGGGCTAAAAAGTTTCTTG+GGG - chr4_4:42489855-42489874 Msa0681800:CDS 35.0%
TGGAAATGTGGAAGATCTTT+AGG - chr4_4:42490242-42490261 Msa0681800:intron 35.0%
GGAAATGTGGAAGATCTTTA+GGG - chr4_4:42490243-42490262 Msa0681800:intron 35.0%
GAAATGTGGAAGATCTTTAG+GGG - chr4_4:42490244-42490263 Msa0681800:intron 35.0%
AAATGTGGAAGATCTTTAGG+GGG - chr4_4:42490245-42490264 Msa0681800:intron 35.0%
CAAGCATCATCTTTAATGAG+CGG + chr4_4:42491118-42491137 None:intergenic 35.0%
GCTCATTAAAGATGATGCTT+GGG - chr4_4:42491117-42491136 Msa0681800:five_prime_UTR 35.0%
CTCATTAAAGATGATGCTTG+GGG - chr4_4:42491118-42491137 Msa0681800:five_prime_UTR 35.0%
TCATTAAAGATGATGCTTGG+GGG - chr4_4:42491119-42491138 Msa0681800:five_prime_UTR 35.0%
ATTGACGCAATGATACGGAT+AGG + chr4_4:42486084-42486103 None:intergenic 40.0%
ACCAAGATGGATCAGAAGAA+TGG - chr4_4:42486322-42486341 Msa0681800:intron 40.0%
CCAAGATGGATCAGAAGAAT+GGG - chr4_4:42486323-42486342 Msa0681800:intron 40.0%
CAAGATGGATCAGAAGAATG+GGG - chr4_4:42486324-42486343 Msa0681800:intron 40.0%
!! TTGCAGAAGCACACATGTTT+TGG - chr4_4:42486486-42486505 Msa0681800:intron 40.0%
GAAGAATCTTCCACTCTGTA+TGG + chr4_4:42486527-42486546 None:intergenic 40.0%
TGGAAGATTCTTCAAAGCCA+TGG - chr4_4:42486534-42486553 Msa0681800:intron 40.0%
AACCAGAGAATTATTGGCCA+TGG + chr4_4:42486554-42486573 None:intergenic 40.0%
CCAATAATTCTCTGGTTGCA+GGG - chr4_4:42486557-42486576 Msa0681800:intron 40.0%
CCCTGTAAGTACTACTTGAT+TGG - chr4_4:42486583-42486602 Msa0681800:intron 40.0%
! ACATCTAATCAGAAGCGTGT+CGG - chr4_4:42486672-42486691 Msa0681800:intron 40.0%
! GGGGCTTCATTGATTAAATC+TGG - chr4_4:42486693-42486712 Msa0681800:intron 40.0%
!! TTTTGAAGAGGTTGGACCTT+TGG - chr4_4:42486819-42486838 Msa0681800:intron 40.0%
TGAAGCCAAGGAATTCAACA+TGG - chr4_4:42486851-42486870 Msa0681800:intron 40.0%
! GAAAGGAGATCTCTTGTTTG+TGG - chr4_4:42486879-42486898 Msa0681800:intron 40.0%
ACTATGACGATTACGGTGAA+CGG + chr4_4:42487125-42487144 None:intergenic 40.0%
ATAACACAGGACAATCCAGT+TGG - chr4_4:42487279-42487298 Msa0681800:CDS 40.0%
AAACTGTATCCTGTTCCAAC+TGG + chr4_4:42487297-42487316 None:intergenic 40.0%
! TGGAACAGGATACAGTTTTG+TGG - chr4_4:42487299-42487318 Msa0681800:CDS 40.0%
!! AACAGGATACAGTTTTGTGG+AGG - chr4_4:42487302-42487321 Msa0681800:CDS 40.0%
TACATTGTGGCAGAGTCTTA+TGG - chr4_4:42487429-42487448 Msa0681800:intron 40.0%
GCTCAAATTATTATCCGCGT+CGG + chr4_4:42487661-42487680 None:intergenic 40.0%
TATGAAGCATAGACACTCCT+CGG + chr4_4:42487754-42487773 None:intergenic 40.0%
ACTTATGTAGGTGTGGCATT+AGG - chr4_4:42487905-42487924 Msa0681800:intron 40.0%
TGTAGTTACAATGCGGTTTG+CGG - chr4_4:42488080-42488099 Msa0681800:intron 40.0%
CTGAATTTGCAGTTGTCATG+GGG - chr4_4:42488147-42488166 Msa0681800:CDS 40.0%
!! TTTGATCTAAAGTGCTGGTG+CGG + chr4_4:42488517-42488536 None:intergenic 40.0%
TGGTTCAGACATATCAGCAA+TGG - chr4_4:42488734-42488753 Msa0681800:intron 40.0%
ATCTTACTTCCCTAAGGACA+AGG - chr4_4:42488802-42488821 Msa0681800:CDS 40.0%
AGAAGAAGACCTTGTCCTTA+GGG + chr4_4:42488814-42488833 None:intergenic 40.0%
AGACTTACGTGACATTCTCA+GGG + chr4_4:42488902-42488921 None:intergenic 40.0%
TAGACTTACGTGACATTCTC+AGG + chr4_4:42488903-42488922 None:intergenic 40.0%
AGTAGCTGATCAACCTGTAA+AGG + chr4_4:42489182-42489201 None:intergenic 40.0%
!! CCAATGTTGACTTGTCTATC+CGG + chr4_4:42489784-42489803 None:intergenic 40.0%
GGAAGGGCTAAAAAGTTTCT+TGG - chr4_4:42489853-42489872 Msa0681800:CDS 40.0%
TTTGTTACCACCACAGTAGA+GGG + chr4_4:42489894-42489913 None:intergenic 40.0%
! ATTTGTTACCACCACAGTAG+AGG + chr4_4:42489895-42489914 None:intergenic 40.0%
TTCTATTGGATTCTTGGAGC+TGG - chr4_4:42489953-42489972 Msa0681800:intron 40.0%
! AATGTGGAAGATCTTTAGGG+GGG - chr4_4:42490246-42490265 Msa0681800:intron 40.0%
ACCTTCATTTGCGCTTAATG+AGG - chr4_4:42490273-42490292 Msa0681800:CDS 40.0%
TCCTCATTAAGCGCAAATGA+AGG + chr4_4:42490277-42490296 None:intergenic 40.0%
CGCTTAATGAGGATCATCTT+AGG - chr4_4:42490284-42490303 Msa0681800:CDS 40.0%
GTAGTGTTTACCGTTGAGTT+TGG - chr4_4:42490599-42490618 Msa0681800:CDS 40.0%
CAAAGGCAAGGGTTGTATTT+GGG + chr4_4:42490807-42490826 None:intergenic 40.0%
TCAAAGGCAAGGGTTGTATT+TGG + chr4_4:42490808-42490827 None:intergenic 40.0%
CTGTATCTCTTCAAAGGCAA+GGG + chr4_4:42490818-42490837 None:intergenic 40.0%
TCTGTATCTCTTCAAAGGCA+AGG + chr4_4:42490819-42490838 None:intergenic 40.0%
CTGTGTCTGTATCTCTTCAA+AGG + chr4_4:42490824-42490843 None:intergenic 40.0%
! TTTCTGATTGAAGGCGTGTT+CGG + chr4_4:42490906-42490925 None:intergenic 40.0%
GTCTGGACATTTCTGATTGA+AGG + chr4_4:42490915-42490934 None:intergenic 40.0%
GTATAAATTGGGAGCTTCGA+CGG - chr4_4:42491048-42491067 Msa0681800:five_prime_UTR 40.0%
CGCTCATTAAAGATGATGCT+TGG - chr4_4:42491116-42491135 Msa0681800:five_prime_UTR 40.0%
TTGGGGGATTTATTTCCCTA+GGG - chr4_4:42491135-42491154 Msa0681800:five_prime_UTR 40.0%
TCATATCCTCTGTCCTTGAC+TGG - chr4_4:42486039-42486058 Msa0681800:CDS 45.0%
GATATATACGCTGCCAGTCA+AGG + chr4_4:42486055-42486074 None:intergenic 45.0%
CCCATTCTTCTGATCCATCT+TGG + chr4_4:42486326-42486345 None:intergenic 45.0%
!! CAGAAGCACACATGTTTTGG+TGG - chr4_4:42486489-42486508 Msa0681800:intron 45.0%
AGCCATGGCCAATAATTCTC+TGG - chr4_4:42486549-42486568 Msa0681800:intron 45.0%
GCCAATAATTCTCTGGTTGC+AGG - chr4_4:42486556-42486575 Msa0681800:intron 45.0%
CCCTGCAACCAGAGAATTAT+TGG + chr4_4:42486560-42486579 None:intergenic 45.0%
ATAATTCTCTGGTTGCAGGG+TGG - chr4_4:42486560-42486579 Msa0681800:intron 45.0%
GATTAGATGTGTCCTGGTGT+CGG + chr4_4:42486662-42486681 None:intergenic 45.0%
GCTTCTGATTAGATGTGTCC+TGG + chr4_4:42486668-42486687 None:intergenic 45.0%
! CATCTAATCAGAAGCGTGTC+GGG - chr4_4:42486673-42486692 Msa0681800:intron 45.0%
! ATCTAATCAGAAGCGTGTCG+GGG - chr4_4:42486674-42486693 Msa0681800:intron 45.0%
TGAATTCCTTGGCTTCAAGC+TGG + chr4_4:42486848-42486867 None:intergenic 45.0%
GACTCTGCCACAATGTACAA+AGG + chr4_4:42487426-42487445 None:intergenic 45.0%
! ATTGTGGCAGAGTCTTATGG+TGG - chr4_4:42487432-42487451 Msa0681800:intron 45.0%
!! GGCAAATTTGCTGTCACTCT+TGG - chr4_4:42487453-42487472 Msa0681800:intron 45.0%
CGCTGCGAACCTTTATATTG+CGG - chr4_4:42488030-42488049 Msa0681800:intron 45.0%
CAAGTCTTGAGACGTCTTTG+AGG + chr4_4:42488179-42488198 None:intergenic 45.0%
TGGAGATCAGTATAGCTCAG+AGG - chr4_4:42488310-42488329 Msa0681800:CDS 45.0%
GACATATCAGCAATGGCAGT+AGG - chr4_4:42488741-42488760 Msa0681800:intron 45.0%
GAGAAGAAGACCTTGTCCTT+AGG + chr4_4:42488815-42488834 None:intergenic 45.0%
CCAAGTTTATCATCACCACC+AGG + chr4_4:42488837-42488856 None:intergenic 45.0%
! CCTGGTGGTGATGATAAACT+TGG - chr4_4:42488834-42488853 Msa0681800:intron 45.0%
GTTGATCAGCTACTGACCAA+GGG - chr4_4:42489188-42489207 Msa0681800:intron 45.0%
!! GATAGTAACATTGGCTCCCT+TGG + chr4_4:42489207-42489226 None:intergenic 45.0%
TACCAAAGGGACTGAAGCTT+GGG - chr4_4:42489505-42489524 Msa0681800:intron 45.0%
CCGGATAGACAAGTCAACAT+TGG - chr4_4:42489781-42489800 Msa0681800:CDS 45.0%
TCTTTATTCCGTAGGTGGGA+AGG - chr4_4:42489836-42489855 Msa0681800:CDS 45.0%
CTTTATTCCGTAGGTGGGAA+GGG - chr4_4:42489837-42489856 Msa0681800:CDS 45.0%
!! TTTTTAGCCCTTCCCACCTA+CGG + chr4_4:42489847-42489866 None:intergenic 45.0%
TTGTTACCACCACAGTAGAG+GGG + chr4_4:42489893-42489912 None:intergenic 45.0%
GGTGGTAACAAATCGACCAA+AGG - chr4_4:42489902-42489921 Msa0681800:intron 45.0%
! ATGTGGAAGATCTTTAGGGG+GGG - chr4_4:42490247-42490266 Msa0681800:intron 45.0%
TCTATGTGAGCAAGAAGGGT+CGG - chr4_4:42490401-42490420 Msa0681800:intron 45.0%
ATTGCTCACGCCAAACTCAA+CGG + chr4_4:42490612-42490631 None:intergenic 45.0%
CTTGGGGGATTTATTTCCCT+AGG - chr4_4:42491134-42491153 Msa0681800:five_prime_UTR 45.0%
! TCCTGGTGTCGGATACACAT+CGG + chr4_4:42486651-42486670 None:intergenic 50.0%
! GTCATTGGATAGGGTGCTTC+AGG - chr4_4:42486781-42486800 Msa0681800:intron 50.0%
! GATAGGGTGCTTCAGGTGTT+GGG - chr4_4:42486788-42486807 Msa0681800:intron 50.0%
GGCTTCAAGCTGGTATCCAA+AGG + chr4_4:42486838-42486857 None:intergenic 50.0%
TGGATACCAGCTTGAAGCCA+AGG - chr4_4:42486839-42486858 Msa0681800:intron 50.0%
CAGGACAATCCAGTTGGAAC+AGG - chr4_4:42487285-42487304 Msa0681800:CDS 50.0%
GCATAGACACTCCTCGGATT+AGG + chr4_4:42487748-42487767 None:intergenic 50.0%
GTGTGGCATTAGGAGACAGT+TGG - chr4_4:42487915-42487934 Msa0681800:intron 50.0%
GTTACAATGCGGTTTGCGGA+CGG - chr4_4:42488084-42488103 Msa0681800:intron 50.0%
! CTTGAGACGTCTTTGAGGAG+AGG + chr4_4:42488174-42488193 None:intergenic 50.0%
!! GTAGCTTTGACGCACAGACA+TGG + chr4_4:42488648-42488667 None:intergenic 50.0%
!! TTCTTGGTCGATTCGGGAAG+TGG - chr4_4:42488714-42488733 Msa0681800:intron 50.0%
AGGACAAGGTCTTCTTCTCC+TGG - chr4_4:42488816-42488835 Msa0681800:CDS 50.0%
ACAAGGTCTTCTTCTCCTGG+TGG - chr4_4:42488819-42488838 Msa0681800:CDS 50.0%
GGTTGATCAGCTACTGACCA+AGG - chr4_4:42489187-42489206 Msa0681800:intron 50.0%
CTACCAAAGGGACTGAAGCT+TGG - chr4_4:42489504-42489523 Msa0681800:intron 50.0%
AACCCAAGCTTCAGTCCCTT+TGG + chr4_4:42489510-42489529 None:intergenic 50.0%
AAGGGACTGAAGCTTGGGTT+AGG - chr4_4:42489510-42489529 Msa0681800:intron 50.0%
AGAAGGGTCGGTCTAACACA+AGG - chr4_4:42490413-42490432 Msa0681800:intron 50.0%
GAAGGGTCGGTCTAACACAA+GGG - chr4_4:42490414-42490433 Msa0681800:intron 50.0%
AAGGGTCGGTCTAACACAAG+GGG - chr4_4:42490415-42490434 Msa0681800:intron 50.0%
GGAGTAGCATGATCTGTCAG+TGG + chr4_4:42491079-42491098 None:intergenic 50.0%
ACCGATGTGTATCCGACACC+AGG - chr4_4:42486647-42486666 Msa0681800:intron 55.0%
! GGATAGGGTGCTTCAGGTGT+TGG - chr4_4:42486787-42486806 Msa0681800:intron 55.0%
!! GGTGCTTCAGGTGTTGGGAT+TGG - chr4_4:42486793-42486812 Msa0681800:intron 55.0%
AAGCGCACAGACACAGACAC+CGG - chr4_4:42487607-42487626 Msa0681800:intron 55.0%
GATTAGGTGTGTCCCGGTGT+CGG + chr4_4:42487732-42487751 None:intergenic 55.0%
! TCCTCGGATTAGGTGTGTCC+CGG + chr4_4:42487738-42487757 None:intergenic 55.0%
GACAGAACACCCCTCTACTG+TGG - chr4_4:42489881-42489900 Msa0681800:intron 55.0%
AGAACACCCCTCTACTGTGG+TGG - chr4_4:42489884-42489903 Msa0681800:intron 55.0%
GATCATGCTACTCCACCTGC+TGG - chr4_4:42491085-42491104 Msa0681800:five_prime_UTR 55.0%
ATGAGCGGCAACAATCCAGC+AGG + chr4_4:42491103-42491122 None:intergenic 55.0%
ACGCTGCCAGTCAAGGACAG+AGG + chr4_4:42486048-42486067 None:intergenic 60.0%
CACAGACACCGGACACGACA+CGG - chr4_4:42487618-42487637 Msa0681800:intron 60.0%
GTCAGTGTCCGTGTCGTGTC+CGG + chr4_4:42487629-42487648 None:intergenic 60.0%
CTGACACTAACACGCCGACG+CGG - chr4_4:42487644-42487663 Msa0681800:CDS 60.0%
TCGGTACACGTGTCCGACAC+CGG - chr4_4:42487716-42487735 Msa0681800:intron 60.0%
ACCGGGACACACCTAATCCG+AGG - chr4_4:42487734-42487753 Msa0681800:intron 60.0%
GCTGGTGCGGAGCAATAGCA+TGG + chr4_4:42488504-42488523 None:intergenic 60.0%
AGCGGCAACAATCCAGCAGG+TGG + chr4_4:42491100-42491119 None:intergenic 60.0%
CGGTACACGTGTCCGACACC+GGG - chr4_4:42487717-42487736 Msa0681800:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 42486004 42491168 42486004 ID=Msa0681800;Name=Msa0681800
chr4_4 mRNA 42486004 42491168 42486004 ID=Msa0681800-mRNA-1;Parent=Msa0681800;Name=Msa0681800-mRNA-1;_AED=0.20;_eAED=0.20;_QI=236|0.91|0.84|0.92|1|1|13|0|460
chr4_4 exon 42486004 42486086 42486004 ID=Msa0681800-mRNA-1:exon:10243;Parent=Msa0681800-mRNA-1
chr4_4 exon 42487198 42487322 42487198 ID=Msa0681800-mRNA-1:exon:10242;Parent=Msa0681800-mRNA-1
chr4_4 exon 42487632 42487684 42487632 ID=Msa0681800-mRNA-1:exon:10241;Parent=Msa0681800-mRNA-1
chr4_4 exon 42487934 42487984 42487934 ID=Msa0681800-mRNA-1:exon:10240;Parent=Msa0681800-mRNA-1
chr4_4 exon 42488108 42488177 42488108 ID=Msa0681800-mRNA-1:exon:10239;Parent=Msa0681800-mRNA-1
chr4_4 exon 42488259 42488470 42488259 ID=Msa0681800-mRNA-1:exon:10238;Parent=Msa0681800-mRNA-1
chr4_4 exon 42488747 42488852 42488747 ID=Msa0681800-mRNA-1:exon:10237;Parent=Msa0681800-mRNA-1
chr4_4 exon 42488946 42489013 42488946 ID=Msa0681800-mRNA-1:exon:10236;Parent=Msa0681800-mRNA-1
chr4_4 exon 42489214 42489257 42489214 ID=Msa0681800-mRNA-1:exon:10235;Parent=Msa0681800-mRNA-1
chr4_4 exon 42489644 42489884 42489644 ID=Msa0681800-mRNA-1:exon:10234;Parent=Msa0681800-mRNA-1
chr4_4 exon 42490272 42490379 42490272 ID=Msa0681800-mRNA-1:exon:10233;Parent=Msa0681800-mRNA-1
chr4_4 exon 42490586 42490680 42490586 ID=Msa0681800-mRNA-1:exon:10232;Parent=Msa0681800-mRNA-1
chr4_4 exon 42490806 42491168 42490806 ID=Msa0681800-mRNA-1:exon:10231;Parent=Msa0681800-mRNA-1
chr4_4 five_prime_UTR 42490933 42491168 42490933 ID=Msa0681800-mRNA-1:five_prime_utr;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42490806 42490932 42490806 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42490586 42490680 42490586 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42490272 42490379 42490272 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42489644 42489884 42489644 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42489214 42489257 42489214 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42488946 42489013 42488946 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42488747 42488852 42488747 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42488259 42488470 42488259 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42488108 42488177 42488108 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42487934 42487984 42487934 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42487632 42487684 42487632 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42487198 42487322 42487198 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
chr4_4 CDS 42486004 42486086 42486004 ID=Msa0681800-mRNA-1:cds;Parent=Msa0681800-mRNA-1
Gene Sequence

>Msa0681800

ATGGAAAAACTTCTTCCTTTTCCCATCATTCTTCTCTTCCTTTCATCATTTTTTCTTCTACAAAACGTTACAGCTTTCAAAAACCAAGATGGATCAGAAGAATGGGGTTATGTTCAAGTCAGACCTAAAGCACACATGTTTTGGTGGCATTATAAAAGTCCATACAGAGTGGAAGATTCTTCAAAGCCATGGCCAATAATTCTCTGGTTGCAGGGTGGCCCTGGTGCTTCAGGTGTTGGGATTGGAAATTTTGAAGAGGTTGGACCTTTGGATACCAGCTTGAAGCCAAGGAATTCAACATGGTTGTTGAAAGGAGATCTCTTGTTTGTGGACAATCCAGTTGGAACAGGATACAGTTTTGTGGAGGATGAGAAACTTTTTGTGAAAACAGATGATGAAGCAGCCGCAGATTTGACAACATTGTTGATTAAATTATTTAATAATGATGAAAAACTTCAAAAGAGTCCTTTGTACATTGTGGCAGAGTCTTATGGTGGCAAATTTGCTGTCACTCTTGGATTATCTGCTCTTAAAGCTATTGAAGACAAGAAATTGATACTTACACTTGGAGGTGTGGCATTAGGAGACAGTTGGATCTCACCAGAAGATTTTGTTTTGTCATGGGGTCCTCTCCTCAAAGACGTCTCAAGACTTGATGATAATGGACTGGAAAAATCAAACAGTATAGCTCAGAGGATCAAGAAGCAACTTGAAGATGGTAAATTTAATGATGCAACTGACTCATGGAGTGAACTTGAGAATCAGATTTCAATAAGCAGCAACAATGTGGATTTTTACAATTTCTTGGTCGATTCGGGAAGTGGTTCAGACATATCAGCAATGGCAGTAGGATTAATAAAAGAAAGATCAAGGAAGAGGTACTCCAAATATCTTACTTCCCTAAGGACAAGGTCTTCTTCTCCTGGTGGTGATGATAAACTTGGCGCTTTACTAAATGGTGTTATAAAGAAGAAGTTAAAAATCATCCCTGAGAATGTCACATGGGGAGGGCAGTCCAATGAAGTTTTCACTTATCTTGAAGGTGATTTTATGAAGCCAAGAATAGATGAGGTTGATCAGCTACTGACCAAGGGAGCCAATGTTACTATCTACAATGGACAAGTTGATCTAATTTGTGCTACCAAAGGGACTGAAGCTTGGGTTAGGAAACTCAAGTGGGAAGGGCTAAAAAGTTTCTTGGGGAAAGACAGAACACCCCTCTACTGTGGTGGTAACAAATCGACCAAAGGATTTTTCAAGTCATACAAAAACCTACAATTCTATTGGATTCTTGGAGCTGATCATGCTACTCCACCTGCTGGATTGTTGCCGCTCATTAAAGATGATGCTTGGGGGATTTATTTCCCTAGGGCGCTAACTTAG
Protein sequence

>Msa0681800

MEKLLPFPIILLFLSSFFLLQNVTAFKNQDGSEEWGYVQVRPKAHMFWWHYKSPYRVEDSSKPWPIILWLQGGPGASGVGIGNFEEVGPLDTSLKPRNSTWLLKGDLLFVDNPVGTGYSFVEDEKLFVKTDDEAAADLTTLLIKLFNNDEKLQKSPLYIVAESYGGKFAVTLGLSALKAIEDKKLILTLGGVALGDSWISPEDFVLSWGPLLKDVSRLDDNGLEKSNSIAQRIKKQLEDGKFNDATDSWSELENQISISSNNVDFYNFLVDSGSGSDISAMAVGLIKERSRKRYSKYLTSLRTRSSSPGGDDKLGALLNGVIKKKLKIIPENVTWGGQSNEVFTYLEGDFMKPRIDEVDQLLTKGANVTIYNGQVDLICATKGTEAWVRKLKWEGLKSFLGKDRTPLYCGGNKSTKGFFKSYKNLQFYWILGADHATPPAGLLPLIKDDAWGIYFPRALT*