AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0569220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0569220 MtrunA17_Chr8g0384311 97.479 119 3 0 168 286 90 208 6.13e-81 243
Msa0569220 MtrunA17_Chr8g0384311 100.000 90 0 0 1 90 1 90 1.24e-57 183
Msa0569220 MtrunA17_Chr3g0130271 92.373 118 8 1 170 286 91 208 4.82e-75 228
Msa0569220 MtrunA17_Chr3g0130271 87.912 91 10 1 1 91 1 90 6.00e-48 159
Msa0569220 MtrunA17_Chr1g0159481 83.333 120 19 1 168 286 94 213 3.80e-69 213
Msa0569220 MtrunA17_Chr1g0159481 91.463 82 7 0 9 90 13 94 3.29e-47 157
Msa0569220 MtrunA17_Chr1g0180291 65.833 120 40 1 168 286 91 210 5.40e-54 174
Msa0569220 MtrunA17_Chr1g0180291 71.605 81 23 0 10 90 11 91 5.82e-35 125
Msa0569220 MtrunA17_Chr7g0265001 62.810 121 42 2 168 286 94 213 8.08e-53 171
Msa0569220 MtrunA17_Chr7g0265001 73.171 82 21 1 10 90 13 94 5.91e-35 125
Msa0569220 MtrunA17_Chr1g0197331 58.824 119 48 1 168 286 94 211 2.03e-43 147
Msa0569220 MtrunA17_Chr1g0197331 67.021 94 27 2 1 90 1 94 1.29e-33 122
Msa0569220 MtrunA17_Chr2g0276931 70.238 84 24 1 8 90 4 87 1.61e-34 124
Msa0569220 MtrunA17_Chr2g0276931 35.294 119 74 3 168 286 87 202 6.71e-14 68.9
Msa0569220 MtrunA17_Chr4g0055651 69.880 83 24 1 9 90 4 86 8.80e-34 122
Msa0569220 MtrunA17_Chr4g0055651 31.933 119 77 3 168 286 86 200 1.07e-13 68.6
Msa0569220 MtrunA17_Chr8g0335471 69.880 83 24 1 9 90 4 86 2.01e-33 121
Msa0569220 MtrunA17_Chr8g0335471 32.773 119 77 3 168 286 86 201 1.60e-12 65.1
Msa0569220 MtrunA17_Chr7g0250531 69.880 83 24 1 9 90 4 86 2.24e-33 121
Msa0569220 MtrunA17_Chr7g0250531 34.454 119 74 3 168 286 86 200 2.27e-14 70.5
Msa0569220 MtrunA17_Chr1g0182321 56.818 88 37 1 4 90 6 93 7.36e-30 112
Msa0569220 MtrunA17_Chr1g0182321 33.858 127 71 6 168 286 93 214 4.32e-11 61.2
Msa0569220 MtrunA17_Chr1g0182301 58.140 86 35 1 6 90 8 93 8.01e-30 112
Msa0569220 MtrunA17_Chr1g0182301 33.858 127 71 6 168 286 93 214 8.27e-11 60.5
Msa0569220 MtrunA17_Chr1g0212521 60.241 83 32 1 9 90 11 93 8.96e-30 112
Msa0569220 MtrunA17_Chr4g0030911 60.241 83 32 1 9 90 11 93 1.01e-29 112
Msa0569220 MtrunA17_Chr7g0263071 60.241 83 32 1 9 90 11 93 1.66e-29 111
Msa0569220 MtrunA17_Chr2g0276941 54.651 86 38 1 6 90 8 93 2.01e-28 108
Msa0569220 MtrunA17_Chr2g0319951 57.831 83 34 1 9 90 9 91 6.01e-28 107
Msa0569220 MtrunA17_Chr2g0327911 54.023 87 39 1 5 90 7 93 1.23e-27 106
Msa0569220 MtrunA17_Chr2g0327911 41.250 80 45 2 168 246 93 171 6.06e-11 60.8
Msa0569220 MtrunA17_Chr3g0112681 55.422 83 36 1 9 90 8 90 1.52e-27 106
Msa0569220 MtrunA17_Chr4g0032201 54.762 84 37 1 8 90 7 90 2.67e-27 105
Msa0569220 MtrunA17_Chr8g0392931 58.333 84 34 1 8 90 9 92 8.98e-27 104
Msa0569220 MtrunA17_Chr2g0325091 57.143 84 35 1 8 90 6 89 9.79e-27 103
Msa0569220 MtrunA17_Chr6g0482721 58.333 84 34 1 8 90 7 90 1.01e-26 103
Msa0569220 MtrunA17_Chr3g0123821 53.488 86 39 1 6 90 9 94 3.21e-26 102
Msa0569220 MtrunA17_Chr1g0189361 45.133 113 48 2 11 109 12 124 4.10e-26 102
Msa0569220 MtrunA17_Chr7g0234201 58.750 80 32 1 12 90 12 91 5.22e-26 102
Msa0569220 MtrunA17_Chr2g0315551 53.012 83 38 1 9 90 9 91 5.52e-26 102
Msa0569220 MtrunA17_Chr4g0046261 49.451 91 45 1 1 90 1 91 1.64e-25 100
Msa0569220 MtrunA17_Chr2g0283131 49.451 91 45 1 1 90 1 91 1.99e-25 100
Msa0569220 MtrunA17_Chr4g0040781 49.451 91 45 1 1 90 1 91 2.46e-25 100
Msa0569220 MtrunA17_Chr8g0346421 52.941 85 39 1 7 90 20 104 4.27e-25 100
Msa0569220 MtrunA17_Chr1g0174281 52.941 85 39 1 7 90 9 93 5.42e-25 99.8
Msa0569220 MtrunA17_Chr7g0275991 53.571 84 38 1 8 90 15 98 8.62e-25 99.4
Msa0569220 MtrunA17_Chr8g0381841 51.163 86 41 1 6 90 10 95 9.53e-25 99.0
Msa0569220 MtrunA17_Chr6g0458081 54.878 82 36 1 10 90 3 84 2.08e-24 97.8
Msa0569220 MtrunA17_Chr6g0458081 42.529 87 48 2 168 254 84 168 2.76e-14 70.5
Msa0569220 MtrunA17_Chr2g0290891 54.878 82 36 1 10 90 3 84 7.46e-24 96.3
Msa0569220 MtrunA17_Chr2g0290891 34.351 131 70 5 168 286 84 210 2.46e-13 67.8
Msa0569220 MtrunA17_Chr7g0231131 53.012 83 38 1 9 90 11 93 2.05e-23 95.5
Msa0569220 MtrunA17_Chr8g0351861 56.098 82 35 1 10 90 13 94 2.38e-23 95.5
Msa0569220 MtrunA17_Chr7g0214241 53.750 80 36 1 12 90 14 93 5.03e-23 94.4
Msa0569220 MtrunA17_Chr8g0349681 55.000 80 35 1 12 90 383 462 8.12e-23 98.2
Msa0569220 MtrunA17_Chr8g0349681 51.250 80 38 1 12 90 12 91 1.53e-18 85.5
Msa0569220 MtrunA17_Chr8g0349681 54.667 75 33 1 12 85 174 248 2.62e-18 84.7
Msa0569220 MtrunA17_Chr8g0349681 31.111 180 106 6 71 246 164 329 9.13e-14 71.2
Msa0569220 MtrunA17_Chr8g0349681 33.333 123 76 3 168 286 462 582 8.31e-13 68.6
Msa0569220 MtrunA17_Chr8g0349681 39.024 82 48 2 168 249 91 170 1.18e-12 67.8
Msa0569220 MtrunA17_Chr8g0349711 53.750 80 36 1 12 90 5 84 3.42e-22 92.0
Msa0569220 MtrunA17_Chr8g0349711 43.038 79 43 2 168 246 84 160 1.24e-14 71.2
Msa0569220 MtrunA17_Chr4g0055081 49.296 71 35 1 14 83 10 80 2.15e-16 75.9
Msa0569220 MtrunA17_Chr5g0400131 49.296 71 35 1 14 83 10 80 2.19e-16 75.9
Msa0569220 MtrunA17_Chr2g0331691 44.304 79 43 1 13 90 10 88 3.69e-16 75.5
Msa0569220 MtrunA17_Chr8g0382221 49.296 71 35 1 14 83 10 80 6.90e-16 74.7
Msa0569220 MtrunA17_Chr3g0123971 49.296 71 35 1 14 83 10 80 7.96e-16 74.3
Msa0569220 MtrunA17_Chr4g0034871 44.304 79 43 1 13 90 8 86 1.18e-15 73.9
Msa0569220 MtrunA17_Chr4g0004661 44.304 79 43 1 13 90 64 142 1.81e-15 74.7
Msa0569220 MtrunA17_Chr8g0383261 41.758 91 51 2 1 90 1 90 1.95e-15 73.6
Msa0569220 MtrunA17_Chr1g0201121 42.500 80 45 1 13 91 8 87 2.15e-15 73.2
Msa0569220 MtrunA17_Chr5g0433561 45.455 77 41 1 15 90 12 88 4.43e-15 72.4
Msa0569220 MtrunA17_Chr5g0433561 31.646 158 91 6 129 286 59 199 8.98e-13 65.9
Msa0569220 MtrunA17_Chr3g0109721 45.455 77 41 1 15 90 12 88 4.90e-15 72.0
Msa0569220 MtrunA17_Chr6g0466301 43.038 79 44 1 13 90 8 86 4.93e-15 72.4
Msa0569220 MtrunA17_Chr6g0465491 43.038 79 44 1 13 90 8 86 4.93e-15 72.4
Msa0569220 MtrunA17_Chr8g0390561 42.857 77 43 1 15 90 11 87 2.27e-14 70.1
Msa0569220 MtrunA17_Chr1g0199331 47.945 73 36 2 15 85 33 105 6.46e-14 68.9
Msa0569220 MtrunA17_Chr1g0199331 33.824 136 77 5 124 259 76 198 3.01e-13 67.0
Msa0569220 MtrunA17_Chr8g0371501 39.286 84 49 2 14 96 7 89 9.47e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0569220 AT1G43890.3 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0569220 AT1G43890.3 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0569220 AT1G43890.1 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0569220 AT1G43890.1 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0569220 AT1G43890.2 83.486 109 18 0 168 276 90 198 1.03e-62 197
Msa0569220 AT1G43890.2 87.778 90 11 0 1 90 1 90 1.85e-48 160
Msa0569220 AT5G03530.2 63.333 120 43 1 168 286 90 209 5.64e-54 174
Msa0569220 AT5G03530.2 73.333 90 24 0 1 90 1 90 1.31e-40 140
Msa0569220 AT5G03530.1 63.333 120 43 1 168 286 90 209 5.64e-54 174
Msa0569220 AT5G03530.1 73.333 90 24 0 1 90 1 90 1.31e-40 140
Msa0569220 AT3G09910.3 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0569220 AT3G09910.3 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0569220 AT3G09910.1 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0569220 AT3G09910.1 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0569220 AT3G09910.2 58.824 119 44 1 168 286 90 203 8.03e-45 151
Msa0569220 AT3G09910.2 70.000 90 27 0 1 90 1 90 8.35e-33 120
Msa0569220 AT3G09910.5 64.516 93 30 1 168 257 90 182 9.75e-37 130
Msa0569220 AT3G09910.5 70.000 90 27 0 1 90 1 90 7.12e-33 120
Msa0569220 AT3G09910.4 64.516 93 30 1 168 257 90 182 9.75e-37 130
Msa0569220 AT3G09910.4 70.000 90 27 0 1 90 1 90 7.12e-33 120
Msa0569220 AT5G47200.1 69.880 83 24 1 9 90 4 86 1.86e-33 121
Msa0569220 AT5G47200.1 33.613 119 75 3 168 286 86 200 1.29e-13 68.6
Msa0569220 AT4G17530.1 69.880 83 24 1 9 90 4 86 2.30e-33 121
Msa0569220 AT4G17530.1 33.613 119 75 3 168 286 86 200 2.16e-13 68.2
Msa0569220 AT1G02130.1 68.675 83 25 1 9 90 4 86 5.82e-33 120
Msa0569220 AT1G02130.1 33.613 119 76 3 168 286 86 201 7.79e-13 66.6
Msa0569220 AT3G11730.1 67.470 83 26 1 9 90 4 86 5.69e-32 117
Msa0569220 AT3G11730.1 33.333 120 77 3 168 286 86 203 2.45e-11 62.4
Msa0569220 AT5G59840.1 60.241 83 32 1 9 90 11 93 1.50e-29 112
Msa0569220 AT3G46060.2 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0569220 AT3G46060.3 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0569220 AT3G46060.1 60.241 83 32 1 9 90 11 93 1.68e-29 111
Msa0569220 AT3G53610.2 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0569220 AT3G53610.3 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0569220 AT3G53610.1 60.241 83 32 1 9 90 11 93 1.78e-29 111
Msa0569220 AT1G06400.1 54.945 91 40 1 1 90 1 91 1.28e-28 109
Msa0569220 AT5G03520.1 57.955 88 36 1 4 90 6 93 1.52e-28 109
Msa0569220 AT3G09900.1 57.955 88 36 1 4 90 6 93 1.89e-28 108
Msa0569220 AT3G09900.1 36.000 125 72 5 168 286 93 215 6.37e-11 61.2
Msa0569220 AT3G46830.1 55.952 84 36 1 8 90 7 90 2.17e-27 106
Msa0569220 AT3G46830.2 55.952 84 36 1 8 90 7 90 2.17e-27 106
Msa0569220 AT1G18200.1 57.143 91 38 1 1 90 1 91 2.24e-27 106
Msa0569220 AT3G07410.1 60.494 81 31 1 11 90 10 90 3.27e-27 105
Msa0569220 AT1G09630.1 54.762 84 37 1 8 90 7 90 3.99e-27 105
Msa0569220 AT1G07410.1 54.762 84 37 1 8 90 7 90 4.26e-27 105
Msa0569220 AT5G59150.1 52.941 85 39 1 7 90 6 90 8.13e-27 104
Msa0569220 AT1G73640.1 54.945 91 40 1 1 90 1 91 1.51e-26 104
Msa0569220 AT1G16920.1 52.941 85 39 1 7 90 7 91 1.82e-26 103
Msa0569220 AT2G33870.1 50.549 91 44 1 1 90 1 91 4.04e-26 102
Msa0569220 AT2G33870.1 33.594 128 74 3 168 286 91 216 9.20e-11 60.8
Msa0569220 AT5G45750.1 54.217 83 37 1 9 90 9 91 4.90e-26 102
Msa0569220 AT5G60860.1 50.549 91 44 1 1 90 1 91 6.72e-26 102
Msa0569220 AT4G39990.1 56.790 81 34 1 11 90 15 95 1.09e-25 102
Msa0569220 AT4G39990.1 31.008 129 77 5 168 286 95 221 7.21e-11 61.2
Msa0569220 AT5G47520.1 56.790 81 34 1 11 90 12 92 1.77e-25 101
Msa0569220 AT4G35860.1 56.098 82 35 1 10 90 3 84 2.01e-25 100
Msa0569220 AT4G35860.1 43.678 87 47 2 168 254 84 168 3.25e-16 75.9
Msa0569220 AT1G28550.1 50.549 91 44 1 1 90 1 91 2.02e-25 100
Msa0569220 AT4G18430.1 50.549 91 44 1 1 90 1 91 3.50e-25 100
Msa0569220 AT2G31680.1 55.556 81 35 1 11 90 10 90 4.90e-25 100
Msa0569220 AT4G18800.1 53.012 83 38 1 9 90 9 91 5.17e-25 99.8
Msa0569220 AT1G05810.1 52.809 89 41 1 3 90 2 90 6.76e-25 99.8
Msa0569220 AT1G05810.1 33.594 128 74 4 168 286 90 215 5.08e-12 64.3
Msa0569220 AT3G12160.1 53.571 84 38 1 8 90 10 93 1.01e-24 99.4
Msa0569220 AT1G01200.1 53.571 84 38 1 8 90 23 106 1.20e-24 99.4
Msa0569220 AT5G65270.1 52.381 84 39 1 8 90 12 95 1.28e-24 99.0
Msa0569220 AT1G05810.2 52.809 89 41 1 3 90 48 136 1.31e-24 100
Msa0569220 AT1G05810.2 33.594 128 74 4 168 286 136 261 1.01e-11 64.3
Msa0569220 AT2G43130.1 54.321 81 36 1 11 90 10 90 1.66e-24 98.6
Msa0569220 AT5G47960.1 52.381 84 39 1 8 90 10 93 2.52e-24 98.2
Msa0569220 AT3G15060.1 47.253 91 47 1 1 90 1 91 3.15e-24 97.8
Msa0569220 AT4G17170.1 54.878 82 36 1 10 90 3 84 1.04e-23 96.3
Msa0569220 AT4G17170.1 36.434 129 70 5 168 286 84 210 2.14e-14 70.9
Msa0569220 AT4G17160.1 54.878 82 36 1 10 90 3 84 5.89e-23 94.0
Msa0569220 AT4G17160.1 38.095 126 66 5 168 286 84 204 1.22e-16 77.0
Msa0569220 AT4G39890.1 48.718 78 39 1 14 90 10 87 1.65e-17 79.7
Msa0569220 AT4G39890.1 34.906 106 66 2 168 271 87 191 2.62e-11 62.4
Msa0569220 AT4G35860.2 43.678 87 47 2 168 254 38 122 1.14e-16 76.3
Msa0569220 AT5G45130.1 43.820 89 47 2 3 90 2 88 1.28e-16 77.0
Msa0569220 AT4G19640.2 46.753 77 40 1 15 90 12 88 1.77e-16 75.1
Msa0569220 AT2G44610.1 49.296 71 35 1 14 83 10 80 2.62e-16 76.3
Msa0569220 AT4G19640.3 46.753 77 40 1 15 90 12 88 2.86e-16 75.9
Msa0569220 AT4G19640.1 46.753 77 40 1 15 90 12 88 4.26e-16 75.5
Msa0569220 AT3G16100.1 45.570 79 42 1 13 90 8 86 4.49e-16 75.5
Msa0569220 AT1G52280.1 45.570 79 42 1 13 90 8 86 5.49e-16 75.5
Msa0569220 AT2G21880.1 44.304 79 43 1 13 90 9 87 5.94e-16 75.5
Msa0569220 AT5G39620.1 46.154 78 41 1 14 90 6 83 8.00e-16 74.7
Msa0569220 AT2G22290.1 49.296 71 35 1 14 83 10 80 1.07e-15 74.3
Msa0569220 AT5G10260.1 49.296 71 35 1 14 83 10 80 1.21e-15 74.3
Msa0569220 AT3G18820.1 44.304 79 43 1 13 90 8 86 1.29e-15 74.3
Msa0569220 AT1G49300.2 44.304 79 43 1 13 90 8 86 2.01e-15 73.6
Msa0569220 AT1G49300.1 44.304 79 43 1 13 90 8 86 2.01e-15 73.6
Msa0569220 AT1G22740.1 43.038 79 44 1 13 90 8 86 2.08e-15 73.6
Msa0569220 AT5G03520.2 60.000 50 20 0 41 90 34 83 5.52e-15 72.4
Msa0569220 AT4G09720.1 47.297 74 38 1 13 85 8 81 7.02e-15 72.4
Msa0569220 AT4G09720.4 47.297 74 38 1 13 85 8 81 7.35e-15 72.4
Msa0569220 AT4G09720.3 47.297 74 38 1 13 85 8 81 8.55e-15 72.4
Msa0569220 AT5G45130.2 45.333 75 40 1 17 90 22 96 8.61e-15 72.0
Msa0569220 AT3G54840.2 47.945 73 36 2 15 85 35 107 5.07e-14 69.7
Msa0569220 AT3G54840.1 47.945 73 36 2 15 85 35 107 7.24e-14 69.3
Msa0569220 AT3G54840.1 33.333 126 72 3 124 249 78 191 7.74e-12 63.5
Msa0569220 AT3G54840.3 47.222 72 36 2 16 85 2 73 1.83e-13 67.4
Msa0569220 AT3G54840.3 33.333 126 72 3 124 249 44 157 4.03e-12 63.5
Msa0569220 AT5G64990.1 46.479 71 37 1 14 83 8 78 2.26e-13 68.2
Msa0569220 AT5G55080.1 41.111 90 48 5 5 90 4 92 9.18e-12 63.9
Msa0569220 AT5G46025.1 38.636 88 53 1 168 255 22 108 1.65e-11 60.5
Msa0569220 AT5G64990.2 42.308 78 37 2 14 83 8 85 7.75e-11 60.8
Msa0569220 AT2G44690.1 39.286 84 49 2 14 96 19 101 8.40e-11 60.8

Find 83 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAATATTGCAGTCATTT+TGG 0.199457 4_1:-71089868 None:intergenic
TTACAAATCTCTCTGAAATA+TGG 0.215859 4_1:+71090044 Msa0569220:CDS
TAGCACTGCATTCAATAAAT+AGG 0.259648 4_1:-71091245 None:intergenic
ATAGACTTTGCTAGGGAATA+TGG 0.277032 4_1:+71091219 Msa0569220:CDS
CACTCAGAGCACATCTTTAC+AGG 0.282566 4_1:-71091939 None:intergenic
AAGCTTGCCATTTGGGATAC+TGG 0.289194 4_1:+71088720 Msa0569220:CDS
TTGTTAATGATTGGGGATTC+TGG 0.299697 4_1:+71088488 Msa0569220:CDS
CCTTAGAGCCCTCGGCTAAA+AGG 0.308198 4_1:-71091605 None:intergenic
ATGTTAGAAATCCATGAAAA+TGG 0.308707 4_1:-71091754 None:intergenic
ATAATAAGATTTGAACATAT+TGG 0.312652 4_1:+71092014 Msa0569220:three_prime_UTR
AGTTTCTTTCTGATAGATAC+TGG 0.314153 4_1:+71088200 Msa0569220:five_prime_UTR
AAAGCTTAAGCTTGCCATTT+GGG 0.317285 4_1:+71088713 Msa0569220:CDS
TGTTCAAATTGTTAATGATT+GGG 0.336729 4_1:+71088480 Msa0569220:CDS
TTTGCAATGGATTCGAGTTC+AGG 0.340964 4_1:+71088437 Msa0569220:exon
GATCGCGGACCCACTAATAT+TGG 0.345728 4_1:+71088222 Msa0569220:five_prime_UTR
ATGTTCAAATTGTTAATGAT+TGG 0.346507 4_1:+71088479 Msa0569220:CDS
AGTCTTGATTTGTTGAATAA+AGG 0.348413 4_1:-71090081 None:intergenic
AAAATACATGATTTGAATAC+TGG 0.350066 4_1:-71089672 None:intergenic
ACCAAATAATGCAGCTGGTC+AGG 0.352646 4_1:+71089064 Msa0569220:intron
ACAGTAAATAAATGTACTAT+TGG 0.357343 4_1:-71089182 None:intergenic
TGGTAAAGCAAATGCATGTT+AGG 0.369362 4_1:+71088177 Msa0569220:five_prime_UTR
AAAAGCTTAAGCTTGCCATT+TGG 0.383397 4_1:+71088712 Msa0569220:CDS
GAAGTTGGAACCTAGAAAAC+TGG 0.389416 4_1:-71091814 None:intergenic
GGATTTCTAACATAATTTGC+TGG 0.390362 4_1:+71091764 Msa0569220:three_prime_UTR
GGACTTTCTTTGTTAGTGAT+GGG 0.390599 4_1:+71089837 Msa0569220:CDS
TGGACTTTCTTTGTTAGTGA+TGG 0.392991 4_1:+71089836 Msa0569220:CDS
AAGAAAGAAACCAATATTAG+TGG 0.394278 4_1:-71088232 None:intergenic
TACAAATCTCTCTGAAATAT+GGG 0.405801 4_1:+71090045 Msa0569220:CDS
GATGATCTCTCTCCCACCAT+TGG 0.416793 4_1:+71088557 Msa0569220:CDS
GACTGCATGAAGATGCTTGT+TGG 0.424153 4_1:+71090100 Msa0569220:CDS
ATGATTGGGGATTCTGGTGT+TGG 0.430250 4_1:+71088494 Msa0569220:CDS
CTCTTGTCATCTTAATCTGT+TGG 0.452730 4_1:+71092043 Msa0569220:three_prime_UTR
TGGATGCAATGCCCTTCTCT+TGG 0.453432 4_1:-71091734 None:intergenic
AAGAGGGAATAGACTTTGCT+AGG 0.457004 4_1:+71091211 Msa0569220:CDS
TAATAATCTGTAGGATTCAA+TGG 0.458814 4_1:+71089807 Msa0569220:intron
TTGTGGGAGGGGATGCTTGT+TGG 0.459665 4_1:+71088157 Msa0569220:five_prime_UTR
GGAACCTAGAAAACTGGAAA+TGG 0.464492 4_1:-71091808 None:intergenic
CTACATATTCGAGACAAAAC+AGG 0.468979 4_1:-71091836 None:intergenic
GTTCAAATTGTTAATGATTG+GGG 0.472425 4_1:+71088481 Msa0569220:CDS
TAATAGTCTGTAGGATTCAA+TGG 0.481798 4_1:+71089649 Msa0569220:intron
GGTCAAATATGTCACGATAG+AGG 0.481991 4_1:+71088686 Msa0569220:CDS
GAAAAGTGGTTGGTTTGCAA+TGG 0.486253 4_1:+71088424 Msa0569220:exon
TTCGAGACAAAACAGGAAGT+TGG 0.491124 4_1:-71091829 None:intergenic
GACTTTCTTTGTTAGTGATG+GGG 0.491805 4_1:+71089838 Msa0569220:CDS
TTGCAATGGATTCGAGTTCA+GGG 0.512395 4_1:+71088438 Msa0569220:exon
TTGTATAGTACCCAATGAAA+AGG 0.512553 4_1:-71088302 None:intergenic
TAGAGTTGTGACAAAGAAAG+AGG 0.518206 4_1:+71091194 Msa0569220:CDS
GTCAAATATGTCACGATAGA+GGG 0.528925 4_1:+71088687 Msa0569220:CDS
TGTTGGAAACAAAGTAGATA+AGG 0.532932 4_1:+71090117 Msa0569220:CDS
GTAGGATTCAATGGCCAAGT+TGG 0.538976 4_1:+71089816 Msa0569220:intron
AGAGTTGTGACAAAGAAAGA+GGG 0.538991 4_1:+71091195 Msa0569220:CDS
TCTTTCATCTAATAGTCTGT+AGG 0.546041 4_1:+71089640 Msa0569220:intron
AAAAGGAAACGAACCAATGG+TGG 0.548019 4_1:-71088570 None:intergenic
TAGTACCCAATGAAAAGGAC+AGG 0.550018 4_1:-71088297 None:intergenic
TCTTACTACAGAGGGGCTCA+AGG 0.553803 4_1:+71089110 Msa0569220:CDS
GAATCACGGTCTCCAAGAGA+AGG 0.560488 4_1:+71091722 Msa0569220:three_prime_UTR
AACAGAATCAGAAAAGTGGT+TGG 0.562276 4_1:+71088414 Msa0569220:intron
TGACAAGTTCTTACTACAGA+GGG 0.566870 4_1:+71089102 Msa0569220:CDS
ATACATACCAGTATCCCAAA+TGG 0.569683 4_1:-71088727 None:intergenic
TCTCTCTGAAATATGGGCAA+AGG 0.571068 4_1:+71090051 Msa0569220:CDS
GAGCCCTCGGCTAAAAGGCT+AGG 0.572458 4_1:-71091600 None:intergenic
AGGGGCTCAAGGAATCATCA+TGG 0.573145 4_1:+71089121 Msa0569220:CDS
AGAGGGAATAGACTTTGCTA+GGG 0.577586 4_1:+71091212 Msa0569220:CDS
GCTTCAGCAACAACCGCTGG+TGG 0.578132 4_1:-71091673 None:intergenic
CATCTTTACAGGTCTTCACA+TGG 0.584085 4_1:-71091928 None:intergenic
AGAAAGAAACCAATATTAGT+GGG 0.587334 4_1:-71088231 None:intergenic
ATAGCTTCAGCAACAACCGC+TGG 0.588465 4_1:-71091676 None:intergenic
AGAGAGATCATCAAAATCAT+CGG 0.592019 4_1:-71088546 None:intergenic
CTAACAAAGAAAGTCCAACT+TGG 0.594978 4_1:-71089830 None:intergenic
GGGGCTCAAGGAATCATCAT+GGG 0.609277 4_1:+71089122 Msa0569220:CDS
TGTGAACAGAATCAGAAAAG+TGG 0.619527 4_1:+71088410 Msa0569220:intron
AAGCAGCATTTCAAGAATCA+CGG 0.626294 4_1:+71091708 Msa0569220:three_prime_UTR
AATCACGGTCTCCAAGAGAA+GGG 0.631244 4_1:+71091723 Msa0569220:three_prime_UTR
GTACTATTGGAAGAATTGAG+AGG 0.635651 4_1:-71089169 None:intergenic
CAGTCTGATGCATCCACCAG+CGG 0.644893 4_1:+71091660 Msa0569220:CDS
ATGTAGATGTAATATAGTGA+CGG 0.650213 4_1:+71091853 Msa0569220:three_prime_UTR
AAAGGAAACGAACCAATGGT+GGG 0.657318 4_1:-71088569 None:intergenic
CTGGTGGATGCATCAGACTG+AGG 0.668797 4_1:-71091657 None:intergenic
GAGATCATCAAAATCATCGG+AGG 0.668909 4_1:-71088543 None:intergenic
TTCTGATAGATACTGGATCG+CGG 0.680015 4_1:+71088207 Msa0569220:five_prime_UTR
CTGACAAGTTCTTACTACAG+AGG 0.693244 4_1:+71089101 Msa0569220:CDS
ATGCAGTTTATGACGTAACA+CGG 0.694182 4_1:+71090011 Msa0569220:intron
GACAAGTTCTTACTACAGAG+GGG 0.732850 4_1:+71089103 Msa0569220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATTTTTGTAATCTTTT+TGG + chr4_1:71088834-71088853 Msa0569220:intron 10.0%
!!! AAACATTTTTGTATAATTGT+GGG + chr4_1:71088141-71088160 Msa0569220:five_prime_UTR 15.0%
!! TAAATCAATTAAGGAATAAA+AGG - chr4_1:71088590-71088609 None:intergenic 15.0%
!! AGATAGAATTAAATCAATTA+AGG - chr4_1:71088599-71088618 None:intergenic 15.0%
!! TGTAAAATGTGAATTAATTT+AGG - chr4_1:71089416-71089435 None:intergenic 15.0%
!! TAATAATCAATATCATCATT+GGG - chr4_1:71089483-71089502 None:intergenic 15.0%
!!! TATTCAAATCATGTATTTTT+TGG + chr4_1:71089676-71089695 Msa0569220:CDS 15.0%
!! ATTTGACATAAAATTCTTAA+AGG - chr4_1:71090147-71090166 None:intergenic 15.0%
!!! AAATTTATCCATGTATTTTT+TGG + chr4_1:71090225-71090244 Msa0569220:intron 15.0%
!! AAAAAATTGAATATCATTAG+AGG + chr4_1:71090783-71090802 Msa0569220:intron 15.0%
!! AAAAAATTGAATATCATTAG+AGG + chr4_1:71090783-71090802 Msa0569220:intron 15.0%
!!! AGTAAAAAAGAACATTTTTA+AGG + chr4_1:71091629-71091648 Msa0569220:CDS 15.0%
!! ATAATAAGATTTGAACATAT+TGG + chr4_1:71092014-71092033 Msa0569220:three_prime_UTR 15.0%
!! ATGTTCAAATTGTTAATGAT+TGG + chr4_1:71088479-71088498 Msa0569220:CDS 20.0%
!! TGTTCAAATTGTTAATGATT+GGG + chr4_1:71088480-71088499 Msa0569220:CDS 20.0%
!! TAAATAAAACTCCAATCTAA+TGG - chr4_1:71088819-71088838 None:intergenic 20.0%
!!! GATATTGGTTTTTAGATTAA+AGG + chr4_1:71088960-71088979 Msa0569220:intron 20.0%
!! AGTACAATATCAATCAAATA+GGG - chr4_1:71089033-71089052 None:intergenic 20.0%
!! AAGTACAATATCAATCAAAT+AGG - chr4_1:71089034-71089053 None:intergenic 20.0%
!! ACAGTAAATAAATGTACTAT+TGG - chr4_1:71089185-71089204 None:intergenic 20.0%
!!! AATTATTTGTTTTCTGAGTA+GGG + chr4_1:71089331-71089350 Msa0569220:intron 20.0%
!! CTAATAATCAATATCATCAT+TGG - chr4_1:71089484-71089503 None:intergenic 20.0%
!! AAAATACATGATTTGAATAC+TGG - chr4_1:71089675-71089694 None:intergenic 20.0%
!! TAAATTAAATACCACATGAA+AGG - chr4_1:71090956-71090975 None:intergenic 20.0%
!!! ATTTAATTTGTGATGAACAA+TGG + chr4_1:71091524-71091543 Msa0569220:intron 20.0%
!! ATTTTTGTATAATTGTGGGA+GGG + chr4_1:71088145-71088164 Msa0569220:five_prime_UTR 25.0%
! AGAAAGAAACCAATATTAGT+GGG - chr4_1:71088234-71088253 None:intergenic 25.0%
! AAGAAAGAAACCAATATTAG+TGG - chr4_1:71088235-71088254 None:intergenic 25.0%
! GTTCAAATTGTTAATGATTG+GGG + chr4_1:71088481-71088500 Msa0569220:CDS 25.0%
! AATGGACAAGGATTTAAAAA+TGG - chr4_1:71088801-71088820 None:intergenic 25.0%
! TTAAAGGCCAATTGTATATA+TGG + chr4_1:71088976-71088995 Msa0569220:intron 25.0%
! TAAAGGCCAATTGTATATAT+GGG + chr4_1:71088977-71088996 Msa0569220:intron 25.0%
! TATTGGGAAGACAATTTAAA+AGG - chr4_1:71089010-71089029 None:intergenic 25.0%
!! TATCAATCAAATAGGGTATT+GGG - chr4_1:71089026-71089045 None:intergenic 25.0%
! ATATCAATCAAATAGGGTAT+TGG - chr4_1:71089027-71089046 None:intergenic 25.0%
! ATATATTCAGTGGATAGAAT+AGG + chr4_1:71089239-71089258 Msa0569220:intron 25.0%
!!! GAATTATTTGTTTTCTGAGT+AGG + chr4_1:71089330-71089349 Msa0569220:intron 25.0%
!! CTTTAAACATGAAGTAGTTT+TGG - chr4_1:71089363-71089382 None:intergenic 25.0%
! TAGGTAAATATTTAACCATG+AGG - chr4_1:71089397-71089416 None:intergenic 25.0%
! TTGATATCATTCATATTGCT+TGG + chr4_1:71089726-71089745 Msa0569220:intron 25.0%
! TGATATCATTCATATTGCTT+GGG + chr4_1:71089727-71089746 Msa0569220:intron 25.0%
! TCTTTCATCTAATAATCTGT+AGG + chr4_1:71089798-71089817 Msa0569220:intron 25.0%
! TAATAATCTGTAGGATTCAA+TGG + chr4_1:71089807-71089826 Msa0569220:intron 25.0%
!! TACAAATATTGCAGTCATTT+TGG - chr4_1:71089871-71089890 None:intergenic 25.0%
! AACAAAATATAGAGGTAATC+AGG - chr4_1:71089981-71090000 None:intergenic 25.0%
!!! TTACAAATCTCTCTGAAATA+TGG + chr4_1:71090044-71090063 Msa0569220:CDS 25.0%
!!! TACAAATCTCTCTGAAATAT+GGG + chr4_1:71090045-71090064 Msa0569220:CDS 25.0%
!!! AGTCTTGATTTGTTGAATAA+AGG - chr4_1:71090084-71090103 None:intergenic 25.0%
! ACATCGTACATAAATATATG+GGG - chr4_1:71090272-71090291 None:intergenic 25.0%
! GACATCGTACATAAATATAT+GGG - chr4_1:71090273-71090292 None:intergenic 25.0%
! AGACATCGTACATAAATATA+TGG - chr4_1:71090274-71090293 None:intergenic 25.0%
!! ATTCAATCGTTAGCAAATTT+TGG + chr4_1:71090363-71090382 Msa0569220:intron 25.0%
!!! TTGTTTTTCTAGTTTGCATT+TGG + chr4_1:71090601-71090620 Msa0569220:intron 25.0%
!! AGTTTCTTTTACATATGTAG+AGG + chr4_1:71090645-71090664 Msa0569220:intron 25.0%
! TTCCTAAAAGTTATCATTTC+CGG - chr4_1:71090763-71090782 None:intergenic 25.0%
! TATACAAATCATGAATTGCA+TGG + chr4_1:71090980-71090999 Msa0569220:intron 25.0%
!!! CTGATTTACTAACCTTATTT+TGG + chr4_1:71091336-71091355 Msa0569220:intron 25.0%
! ATGTTAGAAATCCATGAAAA+TGG - chr4_1:71091757-71091776 None:intergenic 25.0%
! ATGTAGATGTAATATAGTGA+CGG + chr4_1:71091853-71091872 Msa0569220:three_prime_UTR 25.0%
! CATTTTTGTATAATTGTGGG+AGG + chr4_1:71088144-71088163 Msa0569220:five_prime_UTR 30.0%
! TTTTTGTATAATTGTGGGAG+GGG + chr4_1:71088146-71088165 Msa0569220:five_prime_UTR 30.0%
AGTTTCTTTCTGATAGATAC+TGG + chr4_1:71088200-71088219 Msa0569220:five_prime_UTR 30.0%
TTGTATAGTACCCAATGAAA+AGG - chr4_1:71088305-71088324 None:intergenic 30.0%
AGAGAGATCATCAAAATCAT+CGG - chr4_1:71088549-71088568 None:intergenic 30.0%
AATAAAAGGAAACGAACCAA+TGG - chr4_1:71088576-71088595 None:intergenic 30.0%
!!! TATCACAGGTGTTGATTTTA+AGG + chr4_1:71088665-71088684 Msa0569220:intron 30.0%
TAAATCCTTGTCCATTAGAT+TGG + chr4_1:71088805-71088824 Msa0569220:intron 30.0%
AAAGGCCAATTGTATATATG+GGG + chr4_1:71088978-71088997 Msa0569220:intron 30.0%
AAGAATTGAGAGGCAAAAAA+GGG - chr4_1:71089162-71089181 None:intergenic 30.0%
TCTATCCACTGAATATATCA+CGG - chr4_1:71089237-71089256 None:intergenic 30.0%
!! AGCTTCGATTTTGATAAGAA+TGG - chr4_1:71089523-71089542 None:intergenic 30.0%
TCGATATCATTCATATTGCT+TGG + chr4_1:71089568-71089587 Msa0569220:intron 30.0%
CGATATCATTCATATTGCTT+GGG + chr4_1:71089569-71089588 Msa0569220:intron 30.0%
TCTTTCATCTAATAGTCTGT+AGG + chr4_1:71089640-71089659 Msa0569220:intron 30.0%
TAATAGTCTGTAGGATTCAA+TGG + chr4_1:71089649-71089668 Msa0569220:intron 30.0%
CTAACAAATCAATTGTTTCC+AGG - chr4_1:71089770-71089789 None:intergenic 30.0%
GCAATCTGTTTAATGATCTT+TGG + chr4_1:71089897-71089916 Msa0569220:intron 30.0%
!! TGTTTAATGATCTTTGGAGT+TGG + chr4_1:71089903-71089922 Msa0569220:intron 30.0%
!! GTTTAATGATCTTTGGAGTT+GGG + chr4_1:71089904-71089923 Msa0569220:intron 30.0%
!! GCGTGTTGAAAATTTAGTTT+TGG + chr4_1:71089951-71089970 Msa0569220:intron 30.0%
!!! GTTTTGGGAACAAAATATAG+AGG - chr4_1:71089989-71090008 None:intergenic 30.0%
! TCATAAACTGCATCAGTTTT+GGG - chr4_1:71090004-71090023 None:intergenic 30.0%
TGTTGGAAACAAAGTAGATA+AGG + chr4_1:71090117-71090136 Msa0569220:CDS 30.0%
ACTGACATGATTTGAATACA+AGG - chr4_1:71090187-71090206 None:intergenic 30.0%
TAGGCAAACCAAAAAATACA+TGG - chr4_1:71090236-71090255 None:intergenic 30.0%
ATGGGGAACATAGATTATTT+AGG - chr4_1:71090255-71090274 None:intergenic 30.0%
! ACATAGATTTTGAACAACTC+GGG - chr4_1:71090300-71090319 None:intergenic 30.0%
! GACATAGATTTTGAACAACT+CGG - chr4_1:71090301-71090320 None:intergenic 30.0%
TTTCTTCCTTATAGAGAACT+TGG + chr4_1:71090339-71090358 Msa0569220:intron 30.0%
TGAATTCCAAGTTCTCTATA+AGG - chr4_1:71090348-71090367 None:intergenic 30.0%
TTGACACTTCTGCTTATTTA+AGG + chr4_1:71090506-71090525 Msa0569220:intron 30.0%
!! CTGCTTATTTAAGGTGTTTT+TGG + chr4_1:71090515-71090534 Msa0569220:intron 30.0%
TTAATTGCATAACAGATGGA+CGG - chr4_1:71090580-71090599 None:intergenic 30.0%
CAACTTAATTGCATAACAGA+TGG - chr4_1:71090584-71090603 None:intergenic 30.0%
! TCTTTTACATATGTAGAGGT+TGG + chr4_1:71090649-71090668 Msa0569220:intron 30.0%
TAAAAGTTATCATTTCCGGA+GGG - chr4_1:71090759-71090778 None:intergenic 30.0%
TAAACTTTATCATTTCCGGA+GGG - chr4_1:71090850-71090869 None:intergenic 30.0%
TCCGGAAATGATAAAGTTTA+GGG + chr4_1:71090850-71090869 Msa0569220:intron 30.0%
TCCCTAAACTTTATCATTTC+CGG - chr4_1:71090854-71090873 None:intergenic 30.0%
GTAAAAGAAGACAATGAAGA+AGG + chr4_1:71090919-71090938 Msa0569220:intron 30.0%
! CTGTTATCACATTTGTTTCT+AGG + chr4_1:71091055-71091074 Msa0569220:intron 30.0%
! TTTTTCTTGCGTTACATTAG+AGG + chr4_1:71091119-71091138 Msa0569220:intron 30.0%
! ATGTTTGTGCTTGTATTTTC+AGG + chr4_1:71091164-71091183 Msa0569220:intron 30.0%
TAGCACTGCATTCAATAAAT+AGG - chr4_1:71091248-71091267 None:intergenic 30.0%
TGTGAAACGTAAATTCTCTT+TGG + chr4_1:71091369-71091388 Msa0569220:intron 30.0%
GTGAAACGTAAATTCTCTTT+GGG + chr4_1:71091370-71091389 Msa0569220:intron 30.0%
! TGTTTTTCACACAAGTTCAA+TGG + chr4_1:71091463-71091482 Msa0569220:intron 30.0%
! GGATTTCTAACATAATTTGC+TGG + chr4_1:71091764-71091783 Msa0569220:three_prime_UTR 30.0%
TGGTAAAGCAAATGCATGTT+AGG + chr4_1:71088177-71088196 Msa0569220:five_prime_UTR 35.0%
! TTCATTCCTGTCCTTTTCAT+TGG + chr4_1:71088291-71088310 Msa0569220:five_prime_UTR 35.0%
! TCATTCCTGTCCTTTTCATT+GGG + chr4_1:71088292-71088311 Msa0569220:five_prime_UTR 35.0%
TGTGAACAGAATCAGAAAAG+TGG + chr4_1:71088410-71088429 Msa0569220:intron 35.0%
AACAGAATCAGAAAAGTGGT+TGG + chr4_1:71088414-71088433 Msa0569220:intron 35.0%
TTGTTAATGATTGGGGATTC+TGG + chr4_1:71088488-71088507 Msa0569220:CDS 35.0%
GTCAAATATGTCACGATAGA+GGG + chr4_1:71088687-71088706 Msa0569220:CDS 35.0%
AAAAGCTTAAGCTTGCCATT+TGG + chr4_1:71088712-71088731 Msa0569220:CDS 35.0%
! AAAGCTTAAGCTTGCCATTT+GGG + chr4_1:71088713-71088732 Msa0569220:CDS 35.0%
ATACATACCAGTATCCCAAA+TGG - chr4_1:71088730-71088749 None:intergenic 35.0%
! AAACTCCAATCTAATGGACA+AGG - chr4_1:71088813-71088832 None:intergenic 35.0%
!! TTTTGGAGAACCATCTTTAG+CGG + chr4_1:71088851-71088870 Msa0569220:intron 35.0%
ACGAAAAAATGTGGTTGCAA+GGG - chr4_1:71088883-71088902 None:intergenic 35.0%
TTTCAAGCCACGAAAAAATG+TGG - chr4_1:71088892-71088911 None:intergenic 35.0%
TTGAAATGAGACCATGCTTA+AGG + chr4_1:71088906-71088925 Msa0569220:intron 35.0%
TGAAATGAGACCATGCTTAA+GGG + chr4_1:71088907-71088926 Msa0569220:intron 35.0%
CACTTGGAACAATGTGATAT+TGG + chr4_1:71088945-71088964 Msa0569220:intron 35.0%
AAGGCCAATTGTATATATGG+GGG + chr4_1:71088979-71088998 Msa0569220:intron 35.0%
! GTTTTACCAAATAATGCAGC+TGG + chr4_1:71089059-71089078 Msa0569220:intron 35.0%
TGACAAGTTCTTACTACAGA+GGG + chr4_1:71089102-71089121 Msa0569220:CDS 35.0%
GAAGAATTGAGAGGCAAAAA+AGG - chr4_1:71089163-71089182 None:intergenic 35.0%
GTACTATTGGAAGAATTGAG+AGG - chr4_1:71089172-71089191 None:intergenic 35.0%
AAGAATACAGCAATCCAGAT+TGG - chr4_1:71089268-71089287 None:intergenic 35.0%
!!! AAAGCTTTTTTGTTGCCTCA+TGG + chr4_1:71089379-71089398 Msa0569220:intron 35.0%
GAATATCAACCAGTATATCC+TGG + chr4_1:71089591-71089610 Msa0569220:intron 35.0%
! CTAACAAATCAGTTGTTTCC+AGG - chr4_1:71089612-71089631 None:intergenic 35.0%
CAATTGTTTCCAGGATATAC+TGG - chr4_1:71089761-71089780 None:intergenic 35.0%
CTAACAAAGAAAGTCCAACT+TGG - chr4_1:71089833-71089852 None:intergenic 35.0%
TGGACTTTCTTTGTTAGTGA+TGG + chr4_1:71089836-71089855 Msa0569220:CDS 35.0%
GGACTTTCTTTGTTAGTGAT+GGG + chr4_1:71089837-71089856 Msa0569220:CDS 35.0%
GACTTTCTTTGTTAGTGATG+GGG + chr4_1:71089838-71089857 Msa0569220:CDS 35.0%
!! ATATTGCAGTCATTTTGGCA+GGG - chr4_1:71089866-71089885 None:intergenic 35.0%
!! AATATTGCAGTCATTTTGGC+AGG - chr4_1:71089867-71089886 None:intergenic 35.0%
CACGCATCAACTTAAAGAAA+AGG - chr4_1:71089936-71089955 None:intergenic 35.0%
! GTCATAAACTGCATCAGTTT+TGG - chr4_1:71090005-71090024 None:intergenic 35.0%
ATGCAGTTTATGACGTAACA+CGG + chr4_1:71090011-71090030 Msa0569220:intron 35.0%
TAATTGCATAACAGATGGAC+GGG - chr4_1:71090579-71090598 None:intergenic 35.0%
!! TTGTTTTGTGCTTTTGCTTC+TGG + chr4_1:71090711-71090730 Msa0569220:intron 35.0%
CTAAAAGTTATCATTTCCGG+AGG - chr4_1:71090760-71090779 None:intergenic 35.0%
! CTCCGGAAATGATAACTTTT+AGG + chr4_1:71090758-71090777 Msa0569220:intron 35.0%
CTAAACTTTATCATTTCCGG+AGG - chr4_1:71090851-71090870 None:intergenic 35.0%
CTCCGGAAATGATAAAGTTT+AGG + chr4_1:71090849-71090868 Msa0569220:intron 35.0%
TTTGTTTGCATCCTTTCATG+TGG + chr4_1:71090942-71090961 Msa0569220:intron 35.0%
TGTAAAACTGAGAGTCCAAA+TGG - chr4_1:71091009-71091028 None:intergenic 35.0%
TTAGAGGATTTGACATGTGT+TGG + chr4_1:71091135-71091154 Msa0569220:intron 35.0%
!! TTTGTGCTTGTATTTTCAGG+AGG + chr4_1:71091167-71091186 Msa0569220:intron 35.0%
!! TTGTGCTTGTATTTTCAGGA+GGG + chr4_1:71091168-71091187 Msa0569220:intron 35.0%
TAGAGTTGTGACAAAGAAAG+AGG + chr4_1:71091194-71091213 Msa0569220:CDS 35.0%
AGAGTTGTGACAAAGAAAGA+GGG + chr4_1:71091195-71091214 Msa0569220:CDS 35.0%
ATAGACTTTGCTAGGGAATA+TGG + chr4_1:71091219-71091238 Msa0569220:CDS 35.0%
!!! CTTTGAAGAGCTTGTTTTGA+AGG + chr4_1:71091293-71091312 Msa0569220:CDS 35.0%
!!! CTAACCTTATTTTGGTCAGT+AGG + chr4_1:71091344-71091363 Msa0569220:intron 35.0%
TTTCCACCTTCTAATATGCA+TGG - chr4_1:71091401-71091420 None:intergenic 35.0%
GTTCAATGGCTGACAATAAA+TGG + chr4_1:71091477-71091496 Msa0569220:intron 35.0%
!!! GACTGGGGATTTTTTTTCTT+TGG + chr4_1:71091555-71091574 Msa0569220:intron 35.0%
!!! TTTTTTCTTTGGTTGCATGC+AGG + chr4_1:71091566-71091585 Msa0569220:intron 35.0%
AAGCAGCATTTCAAGAATCA+CGG + chr4_1:71091708-71091727 Msa0569220:three_prime_UTR 35.0%
!!! GCTGGTTATTTTAGCATATG+CGG + chr4_1:71091782-71091801 Msa0569220:three_prime_UTR 35.0%
CTACATATTCGAGACAAAAC+AGG - chr4_1:71091839-71091858 None:intergenic 35.0%
CTCTTGTCATCTTAATCTGT+TGG + chr4_1:71092043-71092062 Msa0569220:three_prime_UTR 35.0%
TTCTGATAGATACTGGATCG+CGG + chr4_1:71088207-71088226 Msa0569220:five_prime_UTR 40.0%
TAGTACCCAATGAAAAGGAC+AGG - chr4_1:71088300-71088319 None:intergenic 40.0%
!! GAAAAGTGGTTGGTTTGCAA+TGG + chr4_1:71088424-71088443 Msa0569220:exon 40.0%
! TTTGCAATGGATTCGAGTTC+AGG + chr4_1:71088437-71088456 Msa0569220:exon 40.0%
! TTGCAATGGATTCGAGTTCA+GGG + chr4_1:71088438-71088457 Msa0569220:exon 40.0%
GAGATCATCAAAATCATCGG+AGG - chr4_1:71088546-71088565 None:intergenic 40.0%
AAAGGAAACGAACCAATGGT+GGG - chr4_1:71088572-71088591 None:intergenic 40.0%
AAAAGGAAACGAACCAATGG+TGG - chr4_1:71088573-71088592 None:intergenic 40.0%
CAACACCTGTGATAGATAGT+AGG - chr4_1:71088659-71088678 None:intergenic 40.0%
GGTCAAATATGTCACGATAG+AGG + chr4_1:71088686-71088705 Msa0569220:CDS 40.0%
TTTGGAGAACCATCTTTAGC+GGG + chr4_1:71088852-71088871 Msa0569220:intron 40.0%
CACGAAAAAATGTGGTTGCA+AGG - chr4_1:71088884-71088903 None:intergenic 40.0%
!!! CTTGCAACCACATTTTTTCG+TGG + chr4_1:71088882-71088901 Msa0569220:intron 40.0%
GATCCAAATCCACTTTCACT+TGG + chr4_1:71088929-71088948 Msa0569220:intron 40.0%
! GTTCCAAGTGAAAGTGGATT+TGG - chr4_1:71088935-71088954 None:intergenic 40.0%
CACATTGTTCCAAGTGAAAG+TGG - chr4_1:71088941-71088960 None:intergenic 40.0%
CTCTCCCCCATATATACAAT+TGG - chr4_1:71088986-71089005 None:intergenic 40.0%
CTGACAAGTTCTTACTACAG+AGG + chr4_1:71089101-71089120 Msa0569220:CDS 40.0%
GACAAGTTCTTACTACAGAG+GGG + chr4_1:71089103-71089122 Msa0569220:CDS 40.0%
AGAGGCAAAAAAGGGAGAAT+AGG - chr4_1:71089154-71089173 None:intergenic 40.0%
GATGACCGTGATATATTCAG+TGG + chr4_1:71089229-71089248 Msa0569220:intron 40.0%
GATAGAATAGGATGCCAATC+TGG + chr4_1:71089251-71089270 Msa0569220:intron 40.0%
! TTGTTTTCTGAGTAGGGCTA+AGG + chr4_1:71089337-71089356 Msa0569220:intron 40.0%
!! TCATTTTTCTGACCTCTGCT+GGG + chr4_1:71089445-71089464 Msa0569220:intron 40.0%
! CAGTTGTTTCCAGGATATAC+TGG - chr4_1:71089603-71089622 None:intergenic 40.0%
GAATATCAGCCAGTATATCC+TGG + chr4_1:71089749-71089768 Msa0569220:intron 40.0%
!! TCTCTCTGAAATATGGGCAA+AGG + chr4_1:71090051-71090070 Msa0569220:CDS 40.0%
!! GGAAGAAAACTTTAAAGCGC+TGG - chr4_1:71090327-71090346 None:intergenic 40.0%
CGATCTGCGATTTACTTAGA+AGG - chr4_1:71090409-71090428 None:intergenic 40.0%
TCTAAGTAAATCGCAGATCG+CGG + chr4_1:71090409-71090428 Msa0569220:intron 40.0%
!! ACTTTAGAGAGTACTGCAGA+AGG + chr4_1:71090468-71090487 Msa0569220:intron 40.0%
TGAATTGCATGGACTCCATT+TGG + chr4_1:71090991-71091010 Msa0569220:intron 40.0%
AAGAGGGAATAGACTTTGCT+AGG + chr4_1:71091211-71091230 Msa0569220:CDS 40.0%
AGAGGGAATAGACTTTGCTA+GGG + chr4_1:71091212-71091231 Msa0569220:CDS 40.0%
CACACCTACTGACCAAAATA+AGG - chr4_1:71091351-71091370 None:intergenic 40.0%
GTGCAACCATGCATATTAGA+AGG + chr4_1:71091392-71091411 Msa0569220:intron 40.0%
CAACCATGCATATTAGAAGG+TGG + chr4_1:71091395-71091414 Msa0569220:intron 40.0%
AAGATGAGCCTGTGCAATTT+CGG + chr4_1:71091432-71091451 Msa0569220:intron 40.0%
TTGTGATGAACAATGGCTGA+TGG + chr4_1:71091531-71091550 Msa0569220:intron 40.0%
! GAAGCCATTTCCAGTTTTCT+AGG + chr4_1:71091804-71091823 Msa0569220:three_prime_UTR 40.0%
GGAACCTAGAAAACTGGAAA+TGG - chr4_1:71091811-71091830 None:intergenic 40.0%
GAAGTTGGAACCTAGAAAAC+TGG - chr4_1:71091817-71091836 None:intergenic 40.0%
TTCGAGACAAAACAGGAAGT+TGG - chr4_1:71091832-71091851 None:intergenic 40.0%
CATCTTTACAGGTCTTCACA+TGG - chr4_1:71091931-71091950 None:intergenic 40.0%
ATGATTGGGGATTCTGGTGT+TGG + chr4_1:71088494-71088513 Msa0569220:CDS 45.0%
CGTCTCCTACTATCTATCAC+AGG + chr4_1:71088651-71088670 Msa0569220:intron 45.0%
! AAGCTTGCCATTTGGGATAC+TGG + chr4_1:71088720-71088739 Msa0569220:CDS 45.0%
TTGGAGAACCATCTTTAGCG+GGG + chr4_1:71088853-71088872 Msa0569220:intron 45.0%
GGATTTGGATCCCTTAAGCA+TGG - chr4_1:71088920-71088939 None:intergenic 45.0%
ACCAAATAATGCAGCTGGTC+AGG + chr4_1:71089064-71089083 Msa0569220:intron 45.0%
TCCTGACCAGCTGCATTATT+TGG - chr4_1:71089068-71089087 None:intergenic 45.0%
ATCACGGTCATCAATACTCG+AGG - chr4_1:71089221-71089240 None:intergenic 45.0%
!!! GTCATTTTTCTGACCTCTGC+TGG + chr4_1:71089444-71089463 Msa0569220:intron 45.0%
GTAGGATTCAATGGCCAAGT+TGG + chr4_1:71089816-71089835 Msa0569220:intron 45.0%
GACTGCATGAAGATGCTTGT+TGG + chr4_1:71090100-71090119 Msa0569220:CDS 45.0%
!! TTAGAGAGTACTGCAGAAGG+AGG + chr4_1:71090471-71090490 Msa0569220:intron 45.0%
!! TAGAGAGTACTGCAGAAGGA+GGG + chr4_1:71090472-71090491 Msa0569220:intron 45.0%
! GGTTATCAGCATGTTTCTGC+AGG + chr4_1:71090622-71090641 Msa0569220:intron 45.0%
AGCATGATTGCTTATCCCTC+CGG + chr4_1:71090741-71090760 Msa0569220:intron 45.0%
!!! GAGGAAAGTGCTTTTGCTTC+TGG + chr4_1:71090802-71090821 Msa0569220:intron 45.0%
AGCATGATTGCTTATCCCTC+CGG + chr4_1:71090741-71090760 Msa0569220:intron 45.0%
AGATGAGCCTGTGCAATTTC+GGG + chr4_1:71091433-71091452 Msa0569220:intron 45.0%
! AACAATGGCTGATGGTGACT+GGG + chr4_1:71091539-71091558 Msa0569220:intron 45.0%
! TTTTACTCCCTTAGAGCCCT+CGG - chr4_1:71091616-71091635 None:intergenic 45.0%
AATCACGGTCTCCAAGAGAA+GGG + chr4_1:71091723-71091742 Msa0569220:three_prime_UTR 45.0%
!! GGGCATTGCATCCATTTTCA+TGG + chr4_1:71091743-71091762 Msa0569220:three_prime_UTR 45.0%
CACTCAGAGCACATCTTTAC+AGG - chr4_1:71091942-71091961 None:intergenic 45.0%
GATCGCGGACCCACTAATAT+TGG + chr4_1:71088222-71088241 Msa0569220:five_prime_UTR 50.0%
GATGATCTCTCTCCCACCAT+TGG + chr4_1:71088557-71088576 Msa0569220:CDS 50.0%
TCTTACTACAGAGGGGCTCA+AGG + chr4_1:71089110-71089129 Msa0569220:CDS 50.0%
AGGGGCTCAAGGAATCATCA+TGG + chr4_1:71089121-71089140 Msa0569220:CDS 50.0%
GGGGCTCAAGGAATCATCAT+GGG + chr4_1:71089122-71089141 Msa0569220:CDS 50.0%
TCAGCAAACATTCCCAGCAG+AGG - chr4_1:71089460-71089479 None:intergenic 50.0%
TCGCAGATCGCGGAAAATAG+CGG + chr4_1:71090419-71090438 Msa0569220:intron 50.0%
AGCCTGTGCAATTTCGGGAA+AGG + chr4_1:71091438-71091457 Msa0569220:intron 50.0%
CTCCTTTCCCGAAATTGCAC+AGG - chr4_1:71091443-71091462 None:intergenic 50.0%
! GAACAATGGCTGATGGTGAC+TGG + chr4_1:71091538-71091557 Msa0569220:intron 50.0%
! ACAATGGCTGATGGTGACTG+GGG + chr4_1:71091540-71091559 Msa0569220:intron 50.0%
!! TACACCTAGCCTTTTAGCCG+AGG + chr4_1:71091596-71091615 Msa0569220:CDS 50.0%
!! ACACCTAGCCTTTTAGCCGA+GGG + chr4_1:71091597-71091616 Msa0569220:CDS 50.0%
!! CTTTTAGCCGAGGGCTCTAA+GGG + chr4_1:71091606-71091625 Msa0569220:CDS 50.0%
ATAGCTTCAGCAACAACCGC+TGG - chr4_1:71091679-71091698 None:intergenic 50.0%
GAATCACGGTCTCCAAGAGA+AGG + chr4_1:71091722-71091741 Msa0569220:three_prime_UTR 50.0%
TGGATGCAATGCCCTTCTCT+TGG - chr4_1:71091737-71091756 None:intergenic 50.0%
TTGTGGGAGGGGATGCTTGT+TGG + chr4_1:71088157-71088176 Msa0569220:five_prime_UTR 55.0%
AGGGAAGACCCCGCTAAAGA+TGG - chr4_1:71088864-71088883 None:intergenic 55.0%
AGTACTGCAGAAGGAGGGTG+AGG + chr4_1:71090477-71090496 Msa0569220:intron 55.0%
CCTTAGAGCCCTCGGCTAAA+AGG - chr4_1:71091608-71091627 None:intergenic 55.0%
! CCTTTTAGCCGAGGGCTCTA+AGG + chr4_1:71091605-71091624 Msa0569220:CDS 55.0%
! CTGGTGGATGCATCAGACTG+AGG - chr4_1:71091660-71091679 None:intergenic 55.0%
CAGTCTGATGCATCCACCAG+CGG + chr4_1:71091660-71091679 Msa0569220:CDS 55.0%
GAGCCCTCGGCTAAAAGGCT+AGG - chr4_1:71091603-71091622 None:intergenic 60.0%
GCTTCAGCAACAACCGCTGG+TGG - chr4_1:71091676-71091695 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 71088141 71092075 71088141 ID=Msa0569220;Name=Msa0569220
chr4_1 mRNA 71088141 71092075 71088141 ID=Msa0569220-mRNA-1;Parent=Msa0569220;Name=Msa0569220-mRNA-1;_AED=0.25;_eAED=0.25;_QI=197|0.62|0.55|0.88|0.5|0.55|9|383|286
chr4_1 exon 71088141 71088313 71088141 ID=Msa0569220-mRNA-1:exon:24059;Parent=Msa0569220-mRNA-1
chr4_1 exon 71088419 71088578 71088419 ID=Msa0569220-mRNA-1:exon:24060;Parent=Msa0569220-mRNA-1
chr4_1 exon 71088673 71088741 71088673 ID=Msa0569220-mRNA-1:exon:24061;Parent=Msa0569220-mRNA-1
chr4_1 exon 71089078 71089223 71089078 ID=Msa0569220-mRNA-1:exon:24062;Parent=Msa0569220-mRNA-1
chr4_1 exon 71089655 71089724 71089655 ID=Msa0569220-mRNA-1:exon:24063;Parent=Msa0569220-mRNA-1
chr4_1 exon 71089820 71089903 71089820 ID=Msa0569220-mRNA-1:exon:24064;Parent=Msa0569220-mRNA-1
chr4_1 exon 71090017 71090138 71090017 ID=Msa0569220-mRNA-1:exon:24065;Parent=Msa0569220-mRNA-1
chr4_1 exon 71091186 71091314 71091186 ID=Msa0569220-mRNA-1:exon:24066;Parent=Msa0569220-mRNA-1
chr4_1 exon 71091588 71092075 71091588 ID=Msa0569220-mRNA-1:exon:24067;Parent=Msa0569220-mRNA-1
chr4_1 five_prime_UTR 71088141 71088313 71088141 ID=Msa0569220-mRNA-1:five_prime_utr;Parent=Msa0569220-mRNA-1
chr4_1 five_prime_UTR 71088419 71088442 71088419 ID=Msa0569220-mRNA-1:five_prime_utr;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71088443 71088578 71088443 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71088673 71088741 71088673 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71089078 71089223 71089078 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71089655 71089724 71089655 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71089820 71089903 71089820 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71090017 71090138 71090017 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71091186 71091314 71091186 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 CDS 71091588 71091692 71091588 ID=Msa0569220-mRNA-1:cds;Parent=Msa0569220-mRNA-1
chr4_1 three_prime_UTR 71091693 71092075 71091693 ID=Msa0569220-mRNA-1:three_prime_utr;Parent=Msa0569220-mRNA-1
Gene Sequence

>Msa0569220

ATGGATTCGAGTTCAGGGCAGCAAGAATTCGATTATATGTTCAAATTGTTAATGATTGGGGATTCTGGTGTTGGAAAAAGTAGTCTTCTTCTCAGATTCACCTCCGATGATTTTGATGATCTCTCTCCCACCATTGGTGTTGATTTTAAGGTCAAATATGTCACGATAGAGGGTAAAAAGCTTAAGCTTGCCATTTGGGATACTGCTGGTCAGGAGAGATTTAGAACACTGACAAGTTCTTACTACAGAGGGGCTCAAGGAATCATCATGGGCAAGTTCCTATTCTCCCTTTTTTGCCTCTCAATTCTTCCAATAGTACATTTATTTACTGTGTTATGTGAAATGCCTCGATCTGTAGGATTCAATGGCCAGTATTCAAATCATGTATTTTTTGGCTTTAATGATTTAGATTCTCGTGAAAGATTCAATGGCCAAGTTGGACTTTCTTTGTTAGTGATGGGGTTCCCTGCCAAAATGACTGCAATATTTGTATTAGATGCAATCTTTTATGACGTAACACGGCGAGAAACATTTACAAATCTCTCTGAAATATGGGCAAAGGAAATAGACCTTTATTCAACAAATCAAGACTGCATGAAGATGCTTGTTGGAAACAAAGTAGATAAGGAGGGTGATAGAGTTGTGACAAAGAAAGAGGGAATAGACTTTGCTAGGGAATATGGTTGCCTATTTATTGAATGCAGTGCTAAAACTCGAGTAAATGTGCAGCAATGCTTTGAAGAGCTTGTTTTGAAGGTTCTTGATACACCTAGCCTTTTAGCCGAGGGCTCTAAGGGAGTAAAAAAGAACATTTTTAAGGACAAACCTCAGTCTGATGCATCCACCAGCGGTTGTTGCTGA
Protein sequence

>Msa0569220

MDSSSGQQEFDYMFKLLMIGDSGVGKSSLLLRFTSDDFDDLSPTIGVDFKVKYVTIEGKKLKLAIWDTAGQERFRTLTSSYYRGAQGIIMGKFLFSLFCLSILPIVHLFTVLCEMPRSVGFNGQYSNHVFFGFNDLDSRERFNGQVGLSLLVMGFPAKMTAIFVLDAIFYDVTRRETFTNLSEIWAKEIDLYSTNQDCMKMLVGNKVDKEGDRVVTKKEGIDFAREYGCLFIECSAKTRVNVQQCFEELVLKVLDTPSLLAEGSKGVKKNIFKDKPQSDASTSGCC*