AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0933450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGGTGGAAGAGCATTGT+TGG 0.392371 6_3:+1919317 Msa0933450:intron
AAGAGCATTGTTGGTTTCAG+TGG 0.499187 6_3:+1919326 Msa0933450:CDS
AAGAACATTGTTGATTTCAG+TGG 0.510674 6_3:+1920309 Msa0933450:CDS
TCTCGTCAAAACCGTGGAAG+AGG 0.517503 6_3:+1919019 Msa0933450:CDS
GAGAGAGAGTGAATCAGAAG+AGG 0.523266 6_3:-1918962 None:intergenic
AGCATTGTTGGTTTCAGTGG+AGG 0.538384 6_3:+1919329 Msa0933450:CDS
AAATCAAATATTGAAATCAA+CGG 0.594348 6_3:+1918851 None:intergenic
TTCCTCTCTCGTCAAAACCG+TGG 0.622333 6_3:+1919013 Msa0933450:CDS
AAGAATCCGAACCTCTTCCA+CGG 0.634242 6_3:-1919030 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATTTACCTTTATATT+GGG - chr6_3:1919869-1919888 None:intergenic 15.0%
!! AAATCAATTTACCTTTATAT+TGG - chr6_3:1919870-1919889 None:intergenic 15.0%
!! AATAAGCTGAAAAAAATTTA+TGG + chr6_3:1920008-1920027 Msa0933450:intron 15.0%
!! AATCAATTTACCTTTATATT+GGG - chr6_3:1919869-1919888 None:intergenic 15.0%
!! AAATCAATTTACCTTTATAT+TGG - chr6_3:1919870-1919889 None:intergenic 15.0%
!! AATAAGCTGAAAAAAATTTA+TGG + chr6_3:1920008-1920027 Msa0933450:intron 15.0%
!!! ATTTTGTTGAATGTTAGTTT+GGG + chr6_3:1919374-1919393 Msa0933450:intron 20.0%
!! AAGTTATATTTGTTTGCATA+AGG + chr6_3:1919895-1919914 Msa0933450:intron 20.0%
!!! ATTTTGTTGAATGTTAGTTT+GGG + chr6_3:1919374-1919393 Msa0933450:intron 20.0%
!! AAGTTATATTTGTTTGCATA+AGG + chr6_3:1919895-1919914 Msa0933450:intron 20.0%
!! GATTTTGTTGAATGTTAGTT+TGG + chr6_3:1919373-1919392 Msa0933450:intron 25.0%
!! AAATTTTGTGTTTGAATGCT+AGG + chr6_3:1919409-1919428 Msa0933450:intron 25.0%
!! AATTTTGTGTTTGAATGCTA+GGG + chr6_3:1919410-1919429 Msa0933450:intron 25.0%
! ATAAGCTGAAAACAACTTAT+TGG + chr6_3:1919505-1919524 Msa0933450:intron 25.0%
!! AATTTTCTGTGTTCAGTTTT+AGG + chr6_3:1919580-1919599 Msa0933450:intron 25.0%
!!! TAAGCTATTTCTATTGTGAA+AGG + chr6_3:1919680-1919699 Msa0933450:intron 25.0%
! ATGGTTATGATTTCTATCTT+TGG + chr6_3:1920145-1920164 Msa0933450:intron 25.0%
!! GATTTTGTTGAATGTTAGTT+TGG + chr6_3:1919373-1919392 Msa0933450:intron 25.0%
!! AAATTTTGTGTTTGAATGCT+AGG + chr6_3:1919409-1919428 Msa0933450:intron 25.0%
!! AATTTTGTGTTTGAATGCTA+GGG + chr6_3:1919410-1919429 Msa0933450:intron 25.0%
! ATAAGCTGAAAACAACTTAT+TGG + chr6_3:1919505-1919524 Msa0933450:intron 25.0%
!! AATTTTCTGTGTTCAGTTTT+AGG + chr6_3:1919580-1919599 Msa0933450:intron 25.0%
!!! TAAGCTATTTCTATTGTGAA+AGG + chr6_3:1919680-1919699 Msa0933450:intron 25.0%
! ATGGTTATGATTTCTATCTT+TGG + chr6_3:1920145-1920164 Msa0933450:intron 25.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG + chr6_3:1919300-1919319 Msa0933450:intron 30.0%
TATATCCAAACATGCCATAA+GGG - chr6_3:1920126-1920145 None:intergenic 30.0%
ATATATCCAAACATGCCATA+AGG - chr6_3:1920127-1920146 None:intergenic 30.0%
TTATGGCATGTTTGGATATA+TGG + chr6_3:1920126-1920145 Msa0933450:intron 30.0%
!! TTTCTATCTTTGGTGTTTCA+AGG + chr6_3:1920155-1920174 Msa0933450:intron 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG + chr6_3:1919300-1919319 Msa0933450:intron 30.0%
!! AAGAACATTGTTGATTTCAG+TGG + chr6_3:1920309-1920328 Msa0933450:CDS 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG + chr6_3:1919300-1919319 Msa0933450:intron 30.0%
TATATCCAAACATGCCATAA+GGG - chr6_3:1920126-1920145 None:intergenic 30.0%
ATATATCCAAACATGCCATA+AGG - chr6_3:1920127-1920146 None:intergenic 30.0%
TTATGGCATGTTTGGATATA+TGG + chr6_3:1920126-1920145 Msa0933450:intron 30.0%
!! TTTCTATCTTTGGTGTTTCA+AGG + chr6_3:1920155-1920174 Msa0933450:intron 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG + chr6_3:1919300-1919319 Msa0933450:intron 30.0%
!! AAGAACATTGTTGATTTCAG+TGG + chr6_3:1920309-1920328 Msa0933450:CDS 30.0%
!!! TTTTTTCGTGTTTGATGCAG+TGG + chr6_3:1919146-1919165 Msa0933450:intron 35.0%
!!! GTTCAGTTTTAGGTGATGTA+TGG + chr6_3:1919590-1919609 Msa0933450:intron 35.0%
TATATTGGGACTGTTTGGAT+TGG - chr6_3:1919855-1919874 None:intergenic 35.0%
TCCAAACAGTCCCAATATAA+AGG + chr6_3:1919856-1919875 Msa0933450:intron 35.0%
ACCTTTATATTGGGACTGTT+TGG - chr6_3:1919860-1919879 None:intergenic 35.0%
!! GCTGTTTTCATTAGCTATCT+TGG + chr6_3:1919973-1919992 Msa0933450:intron 35.0%
TTGAGTTTATCCATTAGCAG+AGG - chr6_3:1920083-1920102 None:intergenic 35.0%
ACAATCCAAACAAACCCTTA+TGG + chr6_3:1920109-1920128 Msa0933450:intron 35.0%
AAAAGAAATCCTTGTGTGTG+TGG + chr6_3:1920187-1920206 Msa0933450:intron 35.0%
!!! TTTTTTCGTGTTTGATGCAG+TGG + chr6_3:1919146-1919165 Msa0933450:intron 35.0%
!!! GTTCAGTTTTAGGTGATGTA+TGG + chr6_3:1919590-1919609 Msa0933450:intron 35.0%
TATATTGGGACTGTTTGGAT+TGG - chr6_3:1919855-1919874 None:intergenic 35.0%
TCCAAACAGTCCCAATATAA+AGG + chr6_3:1919856-1919875 Msa0933450:intron 35.0%
ACCTTTATATTGGGACTGTT+TGG - chr6_3:1919860-1919879 None:intergenic 35.0%
!! GCTGTTTTCATTAGCTATCT+TGG + chr6_3:1919973-1919992 Msa0933450:intron 35.0%
TTGAGTTTATCCATTAGCAG+AGG - chr6_3:1920083-1920102 None:intergenic 35.0%
ACAATCCAAACAAACCCTTA+TGG + chr6_3:1920109-1920128 Msa0933450:intron 35.0%
AAAAGAAATCCTTGTGTGTG+TGG + chr6_3:1920187-1920206 Msa0933450:intron 35.0%
!!! AAGAGCATTTTCGACTTCAG+TGG + chr6_3:1919172-1919191 Msa0933450:intron 40.0%
TTCCACGAATTTCTACCTTC+GGG - chr6_3:1919205-1919224 None:intergenic 40.0%
CTTCCACGAATTTCTACCTT+CGG - chr6_3:1919206-1919225 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG + chr6_3:1919303-1919322 Msa0933450:intron 40.0%
!! AAGAGCATTGTTGGTTTCAG+TGG + chr6_3:1919326-1919345 Msa0933450:CDS 40.0%
! GGGTTTAGTGTTTTGTGTTG+TGG + chr6_3:1919430-1919449 Msa0933450:intron 40.0%
! ACATGCCATAAGGGTTTGTT+TGG - chr6_3:1920117-1920136 None:intergenic 40.0%
ACAAACCCTTATGGCATGTT+TGG + chr6_3:1920118-1920137 Msa0933450:intron 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG + chr6_3:1919303-1919322 Msa0933450:intron 40.0%
!! TTCAGTGGAGATTTTGACGA+AGG + chr6_3:1920324-1920343 Msa0933450:CDS 40.0%
!!! AAGAGCATTTTCGACTTCAG+TGG + chr6_3:1919172-1919191 Msa0933450:intron 40.0%
TTCCACGAATTTCTACCTTC+GGG - chr6_3:1919205-1919224 None:intergenic 40.0%
CTTCCACGAATTTCTACCTT+CGG - chr6_3:1919206-1919225 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG + chr6_3:1919303-1919322 Msa0933450:intron 40.0%
!! AAGAGCATTGTTGGTTTCAG+TGG + chr6_3:1919326-1919345 Msa0933450:CDS 40.0%
! GGGTTTAGTGTTTTGTGTTG+TGG + chr6_3:1919430-1919449 Msa0933450:intron 40.0%
! ACATGCCATAAGGGTTTGTT+TGG - chr6_3:1920117-1920136 None:intergenic 40.0%
ACAAACCCTTATGGCATGTT+TGG + chr6_3:1920118-1920137 Msa0933450:intron 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG + chr6_3:1919303-1919322 Msa0933450:intron 40.0%
!! TTCAGTGGAGATTTTGACGA+AGG + chr6_3:1920324-1920343 Msa0933450:CDS 40.0%
GAGAGAGAGTGAATCAGAAG+AGG - chr6_3:1918965-1918984 None:intergenic 45.0%
TCAAAACCGTGGAAGAGGTT+CGG + chr6_3:1919024-1919043 Msa0933450:intron 45.0%
AAGAATCCGAACCTCTTCCA+CGG - chr6_3:1919033-1919052 None:intergenic 45.0%
! TTCAGTGGAGATTTTCCCGA+AGG + chr6_3:1919187-1919206 Msa0933450:intron 45.0%
TTCCCGAAGGTAGAAATTCG+TGG + chr6_3:1919200-1919219 Msa0933450:intron 45.0%
!! AGCATTGTTGGTTTCAGTGG+AGG + chr6_3:1919329-1919348 Msa0933450:CDS 45.0%
!! TTCAGTGGAGGTTTTGACGA+AGG + chr6_3:1919341-1919360 Msa0933450:CDS 45.0%
TCCATTAGCAGAGGTTGTGT+GGG - chr6_3:1920074-1920093 None:intergenic 45.0%
ATCCATTAGCAGAGGTTGTG+TGG - chr6_3:1920075-1920094 None:intergenic 45.0%
AAATCCTTGTGTGTGTGGCA+TGG + chr6_3:1920192-1920211 Msa0933450:intron 45.0%
AATCCTTGTGTGTGTGGCAT+GGG + chr6_3:1920193-1920212 Msa0933450:intron 45.0%
GAGAGAGAGTGAATCAGAAG+AGG - chr6_3:1918965-1918984 None:intergenic 45.0%
TCAAAACCGTGGAAGAGGTT+CGG + chr6_3:1919024-1919043 Msa0933450:intron 45.0%
AAGAATCCGAACCTCTTCCA+CGG - chr6_3:1919033-1919052 None:intergenic 45.0%
! TTCAGTGGAGATTTTCCCGA+AGG + chr6_3:1919187-1919206 Msa0933450:intron 45.0%
TTCCCGAAGGTAGAAATTCG+TGG + chr6_3:1919200-1919219 Msa0933450:intron 45.0%
!! AGCATTGTTGGTTTCAGTGG+AGG + chr6_3:1919329-1919348 Msa0933450:CDS 45.0%
!! TTCAGTGGAGGTTTTGACGA+AGG + chr6_3:1919341-1919360 Msa0933450:CDS 45.0%
TCCATTAGCAGAGGTTGTGT+GGG - chr6_3:1920074-1920093 None:intergenic 45.0%
ATCCATTAGCAGAGGTTGTG+TGG - chr6_3:1920075-1920094 None:intergenic 45.0%
AAATCCTTGTGTGTGTGGCA+TGG + chr6_3:1920192-1920211 Msa0933450:intron 45.0%
AATCCTTGTGTGTGTGGCAT+GGG + chr6_3:1920193-1920212 Msa0933450:intron 45.0%
TTCCTCTCTCGTCAAAACCG+TGG + chr6_3:1919013-1919032 Msa0933450:CDS 50.0%
!! TTCCACGGTTTTGACGAGAG+AGG - chr6_3:1919018-1919037 None:intergenic 50.0%
TCTCGTCAAAACCGTGGAAG+AGG + chr6_3:1919019-1919038 Msa0933450:CDS 50.0%
!! CAGAGGTGGAAGAGCATTGT+TGG + chr6_3:1919317-1919336 Msa0933450:intron 50.0%
GAGGTTGTGTGGGACTGTTT+AGG - chr6_3:1920064-1920083 None:intergenic 50.0%
TCCCACACAACCTCTGCTAA+TGG + chr6_3:1920070-1920089 Msa0933450:intron 50.0%
ATCCTTGTGTGTGTGGCATG+GGG + chr6_3:1920194-1920213 Msa0933450:intron 50.0%
TTCCTCTCTCGTCAAAACCG+TGG + chr6_3:1919013-1919032 Msa0933450:CDS 50.0%
!! TTCCACGGTTTTGACGAGAG+AGG - chr6_3:1919018-1919037 None:intergenic 50.0%
TCTCGTCAAAACCGTGGAAG+AGG + chr6_3:1919019-1919038 Msa0933450:CDS 50.0%
!! CAGAGGTGGAAGAGCATTGT+TGG + chr6_3:1919317-1919336 Msa0933450:intron 50.0%
GAGGTTGTGTGGGACTGTTT+AGG - chr6_3:1920064-1920083 None:intergenic 50.0%
TCCCACACAACCTCTGCTAA+TGG + chr6_3:1920070-1920089 Msa0933450:intron 50.0%
ATCCTTGTGTGTGTGGCATG+GGG + chr6_3:1920194-1920213 Msa0933450:intron 50.0%
AGCCCCATGCCACACACACA+AGG - chr6_3:1920199-1920218 None:intergenic 60.0%
AGCCCCATGCCACACACACA+AGG - chr6_3:1920199-1920218 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 1918865 1920368 1918865 ID=Msa0933450;Name=Msa0933450
chr6_3 mRNA 1918865 1920368 1918865 ID=Msa0933450-mRNA-1;Parent=Msa0933450;Name=Msa0933450-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.5|0.66|1|1|1|3|12|90
chr6_3 exon 1918865 1919040 1918865 ID=Msa0933450-mRNA-1:exon:780;Parent=Msa0933450-mRNA-1
chr6_3 exon 1919320 1919362 1919320 ID=Msa0933450-mRNA-1:exon:781;Parent=Msa0933450-mRNA-1
chr6_3 exon 1920303 1920368 1920303 ID=Msa0933450-mRNA-1:exon:782;Parent=Msa0933450-mRNA-1
chr6_3 CDS 1918865 1919040 1918865 ID=Msa0933450-mRNA-1:cds;Parent=Msa0933450-mRNA-1
chr6_3 CDS 1919320 1919362 1919320 ID=Msa0933450-mRNA-1:cds;Parent=Msa0933450-mRNA-1
chr6_3 CDS 1920303 1920356 1920303 ID=Msa0933450-mRNA-1:cds;Parent=Msa0933450-mRNA-1
chr6_3 three_prime_UTR 1920357 1920368 1920357 ID=Msa0933450-mRNA-1:three_prime_utr;Parent=Msa0933450-mRNA-1
Gene Sequence

>Msa0933450

AATCAACGGTTCAGATCTCTCTATCTCAGCGAGTGTGTGAACGTTGCTGAGTTCACTCAGCGCGTGAACAAAACTCGTTCTTCTTCGTCGTCTTCTTCCTCTTCTGATTCACTCTCTCTCATTCTTCGTTTTCTTCTTCGTTTTCATCTTCCTCTCTCGTCAAAACCGTGGAAGAGAGGTGGAAGAGCATTGTTGGTTTCAGTGGAGGTTTTGACGAAGAGGTGGAAGAACATTGTTGATTTCAGTGGAGATTTTGACGAAGGTAGAATTTAG

Protein sequence

>Msa0933450

NQRFRSLYLSECVNVAEFTQRVNKTRSSSSSSSSSDSLSLILRFLLRFHLPLSSKPWKRGGRALLVSVEVLTKRWKNIVDFSGDFDEGRI*