AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0767100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 6 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAATTCGAAAGCGAATT+AGG 0.199604 5_2:-29657686 None:intergenic
ATTTGAAAATTGAACAAATC+TGG 0.303344 5_2:-29657645 None:intergenic
ATGCACTTCGTGTTAATTAA+CGG 0.398961 5_2:+29657723 Msa0767100:CDS
AAACAGAGTGAAGAAGAGAG+AGG 0.545632 5_2:+29657594 Msa0767100:CDS
ATGATAAGTTTACAATGCAA+CGG 0.588801 5_2:-29659150 None:intergenic
ATTGAGGTTGAGGTCAACAG+TGG 0.665200 5_2:+29659042 Msa0767100:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGATTATTGTGAAAATT+AGG + chr5_2:29657805-29657824 Msa0767100:intron 15.0%
!! CTAAAAATGACAAAAAAATA+AGG - chr5_2:29658401-29658420 None:intergenic 15.0%
!! TTCGTAAGAAAATATAAATA+TGG - chr5_2:29658801-29658820 None:intergenic 15.0%
!! TAAAACATGTAAATACAAAA+AGG - chr5_2:29658944-29658963 None:intergenic 15.0%
!!! TAATTTGTAGATTTTTATTG+AGG + chr5_2:29659026-29659045 Msa0767100:intron 15.0%
!! TTATGATTATTGTGAAAATT+AGG + chr5_2:29657805-29657824 Msa0767100:intron 15.0%
!! CTAAAAATGACAAAAAAATA+AGG - chr5_2:29658401-29658420 None:intergenic 15.0%
!! TTCGTAAGAAAATATAAATA+TGG - chr5_2:29658801-29658820 None:intergenic 15.0%
!! TAAAACATGTAAATACAAAA+AGG - chr5_2:29658944-29658963 None:intergenic 15.0%
!!! TAATTTGTAGATTTTTATTG+AGG + chr5_2:29659026-29659045 Msa0767100:intron 15.0%
!! ATTTGAAAATTGAACAAATC+TGG - chr5_2:29657648-29657667 None:intergenic 20.0%
!! TCGATTGTTATAATTTAGAT+TGG + chr5_2:29657894-29657913 Msa0767100:intron 20.0%
!! TTATAATTTAGATTGGAGTT+AGG + chr5_2:29657901-29657920 Msa0767100:intron 20.0%
!!! ATTTGTTGAGTTAAATGTTA+TGG + chr5_2:29657995-29658014 Msa0767100:intron 20.0%
!! GCTTTATCAATTCAATTTAA+AGG - chr5_2:29658051-29658070 None:intergenic 20.0%
!! AGAGAAATAAACACAAAATA+TGG + chr5_2:29658199-29658218 Msa0767100:intron 20.0%
!! TACATTATTGTATTGTTGAT+TGG + chr5_2:29658225-29658244 Msa0767100:intron 20.0%
!! AATATGGAACAAAAGTAATT+CGG - chr5_2:29658785-29658804 None:intergenic 20.0%
!!! AATTTTGACGTAGAATTTTA+TGG + chr5_2:29658859-29658878 Msa0767100:intron 20.0%
!!! TTTGTATTTACATGTTTTAC+TGG + chr5_2:29658945-29658964 Msa0767100:intron 20.0%
!! ATTTGAAAATTGAACAAATC+TGG - chr5_2:29657648-29657667 None:intergenic 20.0%
!! TCGATTGTTATAATTTAGAT+TGG + chr5_2:29657894-29657913 Msa0767100:intron 20.0%
!! TTATAATTTAGATTGGAGTT+AGG + chr5_2:29657901-29657920 Msa0767100:intron 20.0%
!!! ATTTGTTGAGTTAAATGTTA+TGG + chr5_2:29657995-29658014 Msa0767100:intron 20.0%
!! GCTTTATCAATTCAATTTAA+AGG - chr5_2:29658051-29658070 None:intergenic 20.0%
!! AGAGAAATAAACACAAAATA+TGG + chr5_2:29658199-29658218 Msa0767100:intron 20.0%
!! TACATTATTGTATTGTTGAT+TGG + chr5_2:29658225-29658244 Msa0767100:intron 20.0%
!! AATATGGAACAAAAGTAATT+CGG - chr5_2:29658785-29658804 None:intergenic 20.0%
!!! AATTTTGACGTAGAATTTTA+TGG + chr5_2:29658859-29658878 Msa0767100:intron 20.0%
!!! TTTGTATTTACATGTTTTAC+TGG + chr5_2:29658945-29658964 Msa0767100:intron 20.0%
!! TTGTTCAATTTTCAAATCCA+TGG + chr5_2:29657651-29657670 Msa0767100:CDS 25.0%
! CAATTTAAAGGAGCAAAATA+AGG - chr5_2:29658039-29658058 None:intergenic 25.0%
! TACATTACATTCAATTACGT+TGG - chr5_2:29658112-29658131 None:intergenic 25.0%
! TCGTAAAAAATAGCATATTG+CGG - chr5_2:29658503-29658522 None:intergenic 25.0%
!!! ATGAATAATTTTTCAGTGAC+TGG + chr5_2:29658670-29658689 Msa0767100:intron 25.0%
!!! TGAATAATTTTTCAGTGACT+GGG + chr5_2:29658671-29658690 Msa0767100:intron 25.0%
! TTAGAAGTTATGTAAACTTG+TGG + chr5_2:29658830-29658849 Msa0767100:intron 25.0%
! TAGAAGTTATGTAAACTTGT+GGG + chr5_2:29658831-29658850 Msa0767100:intron 25.0%
!! TTGTTCAATTTTCAAATCCA+TGG + chr5_2:29657651-29657670 Msa0767100:CDS 25.0%
! CAATTTAAAGGAGCAAAATA+AGG - chr5_2:29658039-29658058 None:intergenic 25.0%
! TACATTACATTCAATTACGT+TGG - chr5_2:29658112-29658131 None:intergenic 25.0%
! TCGTAAAAAATAGCATATTG+CGG - chr5_2:29658503-29658522 None:intergenic 25.0%
!!! ATGAATAATTTTTCAGTGAC+TGG + chr5_2:29658670-29658689 Msa0767100:intron 25.0%
!!! TGAATAATTTTTCAGTGACT+GGG + chr5_2:29658671-29658690 Msa0767100:intron 25.0%
! TTAGAAGTTATGTAAACTTG+TGG + chr5_2:29658830-29658849 Msa0767100:intron 25.0%
! TAGAAGTTATGTAAACTTGT+GGG + chr5_2:29658831-29658850 Msa0767100:intron 25.0%
!! TTAGGTTTGAAATTTTGCCA+TGG - chr5_2:29657671-29657690 None:intergenic 30.0%
TTGAAATTCGAAAGCGAATT+AGG - chr5_2:29657689-29657708 None:intergenic 30.0%
ATGCACTTCGTGTTAATTAA+CGG + chr5_2:29657723-29657742 Msa0767100:CDS 30.0%
TTTAGATTGGAGTTAGGTTA+TGG + chr5_2:29657907-29657926 Msa0767100:intron 30.0%
!! AGTTAGGTTATGGTGTTATT+TGG + chr5_2:29657917-29657936 Msa0767100:intron 30.0%
GATTGTGTTAGATATGAGAT+TGG + chr5_2:29657970-29657989 Msa0767100:intron 30.0%
! ATTGAATTGATAAAGCACCT+TGG + chr5_2:29658055-29658074 Msa0767100:intron 30.0%
TGGTTTATTTCACATTTCCA+AGG - chr5_2:29658075-29658094 None:intergenic 30.0%
GTTAATAGTTACACAACACA+CGG + chr5_2:29658266-29658285 Msa0767100:intron 30.0%
TTAATAGTTACACAACACAC+GGG + chr5_2:29658267-29658286 Msa0767100:intron 30.0%
TTATCCAATAGTGACTATAG+CGG + chr5_2:29658527-29658546 Msa0767100:intron 30.0%
! GTAGATTTTTATTGAGGTTG+AGG + chr5_2:29659032-29659051 Msa0767100:intron 30.0%
!! TTAGGTTTGAAATTTTGCCA+TGG - chr5_2:29657671-29657690 None:intergenic 30.0%
TTGAAATTCGAAAGCGAATT+AGG - chr5_2:29657689-29657708 None:intergenic 30.0%
ATGCACTTCGTGTTAATTAA+CGG + chr5_2:29657723-29657742 Msa0767100:CDS 30.0%
TTTAGATTGGAGTTAGGTTA+TGG + chr5_2:29657907-29657926 Msa0767100:intron 30.0%
!! AGTTAGGTTATGGTGTTATT+TGG + chr5_2:29657917-29657936 Msa0767100:intron 30.0%
GATTGTGTTAGATATGAGAT+TGG + chr5_2:29657970-29657989 Msa0767100:intron 30.0%
! ATTGAATTGATAAAGCACCT+TGG + chr5_2:29658055-29658074 Msa0767100:intron 30.0%
TGGTTTATTTCACATTTCCA+AGG - chr5_2:29658075-29658094 None:intergenic 30.0%
GTTAATAGTTACACAACACA+CGG + chr5_2:29658266-29658285 Msa0767100:intron 30.0%
TTAATAGTTACACAACACAC+GGG + chr5_2:29658267-29658286 Msa0767100:intron 30.0%
TTATCCAATAGTGACTATAG+CGG + chr5_2:29658527-29658546 Msa0767100:intron 30.0%
! GTAGATTTTTATTGAGGTTG+AGG + chr5_2:29659032-29659051 Msa0767100:intron 30.0%
ATGTAATGTAATCACACGTG+TGG + chr5_2:29658122-29658141 Msa0767100:intron 35.0%
TGTAATGTAATCACACGTGT+GGG + chr5_2:29658123-29658142 Msa0767100:intron 35.0%
ATTAACCAACGTAGGTAACT+AGG - chr5_2:29658252-29658271 None:intergenic 35.0%
GTGTAACTATTAACCAACGT+AGG - chr5_2:29658260-29658279 None:intergenic 35.0%
AAATAGCTGCGATAGTGTAT+CGG + chr5_2:29658450-29658469 Msa0767100:intron 35.0%
TACATATTGTGCTTCTCAGA+TGG + chr5_2:29658731-29658750 Msa0767100:intron 35.0%
! GTAATTCGGATGCATTTTGT+TGG - chr5_2:29658771-29658790 None:intergenic 35.0%
! ACTTCTGATTCTAGTTGTCT+TGG + chr5_2:29658886-29658905 Msa0767100:intron 35.0%
! CAGCAAAGTGATTCTTTTGT+TGG + chr5_2:29659091-29659110 Msa0767100:CDS 35.0%
! GTGATTCTTTTGTTGGACAA+TGG + chr5_2:29659098-29659117 Msa0767100:CDS 35.0%
ATGTAATGTAATCACACGTG+TGG + chr5_2:29658122-29658141 Msa0767100:intron 35.0%
TGTAATGTAATCACACGTGT+GGG + chr5_2:29658123-29658142 Msa0767100:intron 35.0%
ATTAACCAACGTAGGTAACT+AGG - chr5_2:29658252-29658271 None:intergenic 35.0%
GTGTAACTATTAACCAACGT+AGG - chr5_2:29658260-29658279 None:intergenic 35.0%
AAATAGCTGCGATAGTGTAT+CGG + chr5_2:29658450-29658469 Msa0767100:intron 35.0%
TACATATTGTGCTTCTCAGA+TGG + chr5_2:29658731-29658750 Msa0767100:intron 35.0%
! GTAATTCGGATGCATTTTGT+TGG - chr5_2:29658771-29658790 None:intergenic 35.0%
! ACTTCTGATTCTAGTTGTCT+TGG + chr5_2:29658886-29658905 Msa0767100:intron 35.0%
! CAGCAAAGTGATTCTTTTGT+TGG + chr5_2:29659091-29659110 Msa0767100:CDS 35.0%
! GTGATTCTTTTGTTGGACAA+TGG + chr5_2:29659098-29659117 Msa0767100:CDS 35.0%
AAACAGAGTGAAGAAGAGAG+AGG + chr5_2:29657594-29657613 Msa0767100:CDS 40.0%
!! CGTTGGTAATAGTGCTTCAT+TGG - chr5_2:29658095-29658114 None:intergenic 40.0%
AACACCGCTATAGTCACTAT+TGG - chr5_2:29658534-29658553 None:intergenic 40.0%
! TGCCATAACAAGTGTTGTCA+TGG - chr5_2:29658595-29658614 None:intergenic 40.0%
GCTCTATAGTGTCTATAACG+TGG + chr5_2:29658638-29658657 Msa0767100:intron 40.0%
ATATTGTGCTTCTCAGATGG+TGG + chr5_2:29658734-29658753 Msa0767100:intron 40.0%
AAACAGAGTGAAGAAGAGAG+AGG + chr5_2:29657594-29657613 Msa0767100:CDS 40.0%
!! CGTTGGTAATAGTGCTTCAT+TGG - chr5_2:29658095-29658114 None:intergenic 40.0%
AACACCGCTATAGTCACTAT+TGG - chr5_2:29658534-29658553 None:intergenic 40.0%
! TGCCATAACAAGTGTTGTCA+TGG - chr5_2:29658595-29658614 None:intergenic 40.0%
GCTCTATAGTGTCTATAACG+TGG + chr5_2:29658638-29658657 Msa0767100:intron 40.0%
ATATTGTGCTTCTCAGATGG+TGG + chr5_2:29658734-29658753 Msa0767100:intron 40.0%
GATGGAATTGTGTCTGACAC+GGG - chr5_2:29658162-29658181 None:intergenic 45.0%
GGATGGAATTGTGTCTGACA+CGG - chr5_2:29658163-29658182 None:intergenic 45.0%
CACAATTCCATCCGAAGTGT+CGG + chr5_2:29658170-29658189 Msa0767100:intron 45.0%
!! TCTCTATAGCACCGACACTT+CGG - chr5_2:29658184-29658203 None:intergenic 45.0%
TTGGACCTAGTTACCTACGT+TGG + chr5_2:29658244-29658263 Msa0767100:intron 45.0%
TCGCAGCTATTTGACAACAC+TGG - chr5_2:29658442-29658461 None:intergenic 45.0%
! CGCCATGACAACACTTGTTA+TGG + chr5_2:29658590-29658609 Msa0767100:intron 45.0%
ATTGAGGTTGAGGTCAACAG+TGG + chr5_2:29659042-29659061 Msa0767100:intron 45.0%
GATGGAATTGTGTCTGACAC+GGG - chr5_2:29658162-29658181 None:intergenic 45.0%
GGATGGAATTGTGTCTGACA+CGG - chr5_2:29658163-29658182 None:intergenic 45.0%
CACAATTCCATCCGAAGTGT+CGG + chr5_2:29658170-29658189 Msa0767100:intron 45.0%
!! TCTCTATAGCACCGACACTT+CGG - chr5_2:29658184-29658203 None:intergenic 45.0%
TTGGACCTAGTTACCTACGT+TGG + chr5_2:29658244-29658263 Msa0767100:intron 45.0%
TCGCAGCTATTTGACAACAC+TGG - chr5_2:29658442-29658461 None:intergenic 45.0%
! CGCCATGACAACACTTGTTA+TGG + chr5_2:29658590-29658609 Msa0767100:intron 45.0%
ATTGAGGTTGAGGTCAACAG+TGG + chr5_2:29659042-29659061 Msa0767100:intron 45.0%
!! TATAGCACCGACACTTCGGA+TGG - chr5_2:29658180-29658199 None:intergenic 50.0%
!! TATAGCACCGACACTTCGGA+TGG - chr5_2:29658180-29658199 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 29657591 29659160 29657591 ID=Msa0767100;Name=Msa0767100
chr5_2 mRNA 29657591 29659160 29657591 ID=Msa0767100-mRNA-1;Parent=Msa0767100;Name=Msa0767100-mRNA-1;_AED=0.41;_eAED=0.65;_QI=0|0|0.5|1|0|0|2|0|87
chr5_2 exon 29657591 29657744 29657591 ID=Msa0767100-mRNA-1:exon:11306;Parent=Msa0767100-mRNA-1
chr5_2 exon 29659054 29659160 29659054 ID=Msa0767100-mRNA-1:exon:11307;Parent=Msa0767100-mRNA-1
chr5_2 CDS 29657591 29657744 29657591 ID=Msa0767100-mRNA-1:cds;Parent=Msa0767100-mRNA-1
chr5_2 CDS 29659054 29659160 29659054 ID=Msa0767100-mRNA-1:cds;Parent=Msa0767100-mRNA-1
Gene Sequence

>Msa0767100

AAAAAACAGAGTGAAGAAGAGAGAGGAAAATCTCTACTCTTTTCTCGCTTCATTCCAGATTTGTTCAATTTTCAAATCCATGGCAAAATTTCAAACCTAATTCGCTTTCGAATTTCAATTCATTCTCAAATCATGCACTTCGTGTTAATTAACGGTCAACAGTGGAAGCTAATAGCTGAGACTCAGTTTCACAGCAAAGTGATTCTTTTGTTGGACAATGGCTTATATACTGATATTGCTGTGATTTCTGCCGTTGCATTG

Protein sequence

>Msa0767100

KKQSEEERGKSLLFSRFIPDLFNFQIHGKISNLIRFRISIHSQIMHFVLINGQQWKLIAETQFHSKVILLLDNGLYTDIAVISAVAL