AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423200 MtrunA17_Chr3g0124111 86.935 199 26 0 1 199 1 199 2.93e-132 369
Msa0423200 MtrunA17_Chr3g0124141 86.935 199 26 0 1 199 1 199 2.14e-129 362
Msa0423200 MtrunA17_Chr3g0124061 83.920 199 32 0 1 199 1 199 1.22e-125 352
Msa0423200 MtrunA17_Chr3g0124091 84.080 201 29 2 1 199 1 200 1.75e-123 347
Msa0423200 MtrunA17_Chr3g0124151 83.019 106 18 0 94 199 1 106 6.11e-61 185
Msa0423200 MtrunA17_Chr3g0124131 88.235 51 6 0 1 51 1 51 2.73e-27 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423200 AT5G65030.1 31.472 197 110 7 11 187 10 201 8.57e-25 96.7
Msa0423200 AT5G10210.1 30.808 198 111 8 15 199 13 197 4.70e-24 94.4

Find 38 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGCCAAATCATTGCCCTTT+TGG 0.190841 3_2:+72965682 Msa0423200:CDS
ATATGGGGCTCCAAACTAAT+TGG 0.223958 3_2:+72965798 Msa0423200:CDS
ACACTAGGCTTTGTTGATTA+AGG 0.234215 3_2:-72965755 None:intergenic
CTCACCTTTCCCAATTAGTT+TGG 0.258127 3_2:-72965808 None:intergenic
TTAATCTCCACTTCCACTTC+TGG 0.281665 3_2:-72965936 None:intergenic
AGCTAAGGCAAAGGAAGATA+TGG 0.333930 3_2:+72965781 Msa0423200:CDS
TGCACTTGGCGCAGCTTTGG+AGG 0.390376 3_2:+72966088 Msa0423200:CDS
GTCCCAAAAGGGCAATGATT+TGG 0.392085 3_2:-72965685 None:intergenic
ATTTGCACTTGGCGCAGCTT+TGG 0.400082 3_2:+72966085 Msa0423200:CDS
GATTCAAGAATCACTTTCCA+TGG 0.437928 3_2:-72965834 None:intergenic
TATGGGGCTCCAAACTAATT+GGG 0.456932 3_2:+72965799 Msa0423200:CDS
GCTAAGGCAAAGGAAGATAT+GGG 0.457270 3_2:+72965782 Msa0423200:CDS
AAAATGGTTGAAGATGGATT+TGG 0.470551 3_2:+72965875 Msa0423200:CDS
AGTAGCTTCGGTTGCTGAGA+TGG 0.471131 3_2:+72965965 Msa0423200:CDS
GATTAAAATAAAAGTAGCTT+CGG 0.474275 3_2:+72965953 Msa0423200:CDS
ACTAAATGACTCGTCCCAAA+AGG 0.477554 3_2:-72965697 None:intergenic
CTGTGAATTGAGAATAATAC+AGG 0.480110 3_2:+72965557 Msa0423200:CDS
TTGGGAAAGGTGAGATTCCA+TGG 0.490587 3_2:+72965817 Msa0423200:CDS
AAAGCCTAGTGTTGGAGCTA+AGG 0.499139 3_2:+72965766 Msa0423200:CDS
TTTGCCTTAGCTCCAACACT+AGG 0.507447 3_2:-72965770 None:intergenic
AGATGGAGAAGCAAAATAAG+AGG 0.509817 3_2:+72965982 Msa0423200:CDS
CTAAATGACTCGTCCCAAAA+GGG 0.528554 3_2:-72965696 None:intergenic
ATGAATAATTTGAATGAGTG+TGG 0.533229 3_2:+72966008 Msa0423200:CDS
ACGGCCATGACCATCGTGCT+TGG 0.538014 3_2:+72966039 Msa0423200:CDS
CCAAAAGGGCAATGATTTGG+CGG 0.551334 3_2:-72965682 None:intergenic
TAATCAACAAAGCCTAGTGT+TGG 0.564525 3_2:+72965758 Msa0423200:CDS
AGCACCAAGCACGATGGTCA+TGG 0.566327 3_2:-72966043 None:intergenic
AGTCGAAGTGATTGCAAAGA+GGG 0.568276 3_2:+72965900 Msa0423200:CDS
CATAACGCCAGAAGTGGAAG+TGG 0.570407 3_2:+72965929 Msa0423200:CDS
GATTCTTGAATCACAAAACA+TGG 0.583125 3_2:+72965845 Msa0423200:CDS
TATGTTCATAACGCCAGAAG+TGG 0.583659 3_2:+72965923 Msa0423200:CDS
ACATATGAAGATACATGAGA+TGG 0.585374 3_2:-72965525 None:intergenic
TAGTGTTGGAGCTAAGGCAA+AGG 0.590191 3_2:+72965772 Msa0423200:CDS
GATTGTGATATATTTGCACT+TGG 0.591421 3_2:+72966074 Msa0423200:CDS
GGCTCCAAACTAATTGGGAA+AGG 0.604038 3_2:+72965804 Msa0423200:CDS
GAGTCGAAGTGATTGCAAAG+AGG 0.661989 3_2:+72965899 Msa0423200:CDS
CAGCGCAGCACCAAGCACGA+TGG 0.681591 3_2:-72966049 None:intergenic
CTAAGGCAAAGGAAGATATG+GGG 0.687073 3_2:+72965783 Msa0423200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTAAAATAAAAGTAGCTT+CGG + chr3_2:72965953-72965972 Msa0423200:CDS 20.0%
! AAAACATGGAGCTAAAAAAA+TGG + chr3_2:72965859-72965878 Msa0423200:CDS 25.0%
! ATGAATAATTTGAATGAGTG+TGG + chr3_2:72966008-72966027 Msa0423200:CDS 25.0%
ACATATGAAGATACATGAGA+TGG - chr3_2:72965528-72965547 None:intergenic 30.0%
CTGTGAATTGAGAATAATAC+AGG + chr3_2:72965557-72965576 Msa0423200:CDS 30.0%
!!! ATTCTTTTGTTGTTCTCTGT+TGG - chr3_2:72965639-72965658 None:intergenic 30.0%
! AAGGTTTTCCATGAAATCTT+GGG - chr3_2:72965739-72965758 None:intergenic 30.0%
! TAAGGTTTTCCATGAAATCT+TGG - chr3_2:72965740-72965759 None:intergenic 30.0%
GATTCTTGAATCACAAAACA+TGG + chr3_2:72965845-72965864 Msa0423200:CDS 30.0%
GCTAAAAAAATGGTTGAAGA+TGG + chr3_2:72965869-72965888 Msa0423200:CDS 30.0%
! AAAATGGTTGAAGATGGATT+TGG + chr3_2:72965875-72965894 Msa0423200:CDS 30.0%
GATTGTGATATATTTGCACT+TGG + chr3_2:72966074-72966093 Msa0423200:CDS 30.0%
! AGGTTTTCCATGAAATCTTG+GGG - chr3_2:72965738-72965757 None:intergenic 35.0%
!! ACACTAGGCTTTGTTGATTA+AGG - chr3_2:72965758-72965777 None:intergenic 35.0%
TAATCAACAAAGCCTAGTGT+TGG + chr3_2:72965758-72965777 Msa0423200:CDS 35.0%
GATTCAAGAATCACTTTCCA+TGG - chr3_2:72965837-72965856 None:intergenic 35.0%
AGATGGAGAAGCAAAATAAG+AGG + chr3_2:72965982-72966001 Msa0423200:CDS 35.0%
AATGAGTGTGGATGCAAAAA+CGG + chr3_2:72966020-72966039 Msa0423200:CDS 35.0%
! CGACGAAAAACTTGTTTGCA+AGG + chr3_2:72965604-72965623 Msa0423200:CDS 40.0%
CTAAATGACTCGTCCCAAAA+GGG - chr3_2:72965699-72965718 None:intergenic 40.0%
ACTAAATGACTCGTCCCAAA+AGG - chr3_2:72965700-72965719 None:intergenic 40.0%
! GGTTTTCCATGAAATCTTGG+GGG - chr3_2:72965737-72965756 None:intergenic 40.0%
AGCTAAGGCAAAGGAAGATA+TGG + chr3_2:72965781-72965800 Msa0423200:CDS 40.0%
GCTAAGGCAAAGGAAGATAT+GGG + chr3_2:72965782-72965801 Msa0423200:CDS 40.0%
CTAAGGCAAAGGAAGATATG+GGG + chr3_2:72965783-72965802 Msa0423200:CDS 40.0%
ATATGGGGCTCCAAACTAAT+TGG + chr3_2:72965798-72965817 Msa0423200:CDS 40.0%
TATGGGGCTCCAAACTAATT+GGG + chr3_2:72965799-72965818 Msa0423200:CDS 40.0%
CTCACCTTTCCCAATTAGTT+TGG - chr3_2:72965811-72965830 None:intergenic 40.0%
AGTCGAAGTGATTGCAAAGA+GGG + chr3_2:72965900-72965919 Msa0423200:CDS 40.0%
TATGTTCATAACGCCAGAAG+TGG + chr3_2:72965923-72965942 Msa0423200:CDS 40.0%
TTAATCTCCACTTCCACTTC+TGG - chr3_2:72965939-72965958 None:intergenic 40.0%
! CCAAAAGGGCAATGATTTGG+CGG - chr3_2:72965685-72965704 None:intergenic 45.0%
! CGCCAAATCATTGCCCTTTT+GGG + chr3_2:72965683-72965702 Msa0423200:CDS 45.0%
GTCCCAAAAGGGCAATGATT+TGG - chr3_2:72965688-72965707 None:intergenic 45.0%
TTCTTGCCCCCAAGATTTCA+TGG + chr3_2:72965728-72965747 Msa0423200:CDS 45.0%
! AAAGCCTAGTGTTGGAGCTA+AGG + chr3_2:72965766-72965785 Msa0423200:CDS 45.0%
TTTGCCTTAGCTCCAACACT+AGG - chr3_2:72965773-72965792 None:intergenic 45.0%
! TAGTGTTGGAGCTAAGGCAA+AGG + chr3_2:72965772-72965791 Msa0423200:CDS 45.0%
GGCTCCAAACTAATTGGGAA+AGG + chr3_2:72965804-72965823 Msa0423200:CDS 45.0%
TTGGGAAAGGTGAGATTCCA+TGG + chr3_2:72965817-72965836 Msa0423200:CDS 45.0%
GAGTCGAAGTGATTGCAAAG+AGG + chr3_2:72965899-72965918 Msa0423200:CDS 45.0%
! CCGCCAAATCATTGCCCTTT+TGG + chr3_2:72965682-72965701 Msa0423200:CDS 50.0%
CATAACGCCAGAAGTGGAAG+TGG + chr3_2:72965929-72965948 Msa0423200:CDS 50.0%
AGTAGCTTCGGTTGCTGAGA+TGG + chr3_2:72965965-72965984 Msa0423200:CDS 50.0%
ATTTGCACTTGGCGCAGCTT+TGG + chr3_2:72966085-72966104 Msa0423200:CDS 50.0%
AGCACCAAGCACGATGGTCA+TGG - chr3_2:72966046-72966065 None:intergenic 55.0%
ACGGCCATGACCATCGTGCT+TGG + chr3_2:72966039-72966058 Msa0423200:CDS 60.0%
! TGCACTTGGCGCAGCTTTGG+AGG + chr3_2:72966088-72966107 Msa0423200:CDS 60.0%
CAGCGCAGCACCAAGCACGA+TGG - chr3_2:72966052-72966071 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 72965519 72966118 72965519 ID=Msa0423200;Name=Msa0423200
chr3_2 mRNA 72965519 72966118 72965519 ID=Msa0423200-mRNA-1;Parent=Msa0423200;Name=Msa0423200-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|199
chr3_2 exon 72965519 72966118 72965519 ID=Msa0423200-mRNA-1:exon:18107;Parent=Msa0423200-mRNA-1
chr3_2 CDS 72965519 72966118 72965519 ID=Msa0423200-mRNA-1:cds;Parent=Msa0423200-mRNA-1
Gene Sequence

>Msa0423200

ATGGATCCATCTCATGTATCTTCATATGTAAACTTCAGCTGTGAATTGAGAATAATACAGGCTCGAAACATTGAATTCATCAAGACGACGAAAAACTTGTTTGCAAGGTTGTATCTTCCAACAGAGAACAACAAAAGAATTCAACTCAACAGCAAAAATGTTTCCGCCAAATCATTGCCCTTTTGGGACGAGTCATTTAGTCTAGATTGTTCTTGCCCCCAAGATTTCATGGAAAACCTTAATCAACAAAGCCTAGTGTTGGAGCTAAGGCAAAGGAAGATATGGGGCTCCAAACTAATTGGGAAAGGTGAGATTCCATGGAAAGTGATTCTTGAATCACAAAACATGGAGCTAAAAAAATGGTTGAAGATGGATTTGGTGAGTCGAAGTGATTGCAAAGAGGGTATGTTCATAACGCCAGAAGTGGAAGTGGAGATTAAAATAAAAGTAGCTTCGGTTGCTGAGATGGAGAAGCAAAATAAGAGGAGAATGAATAATTTGAATGAGTGTGGATGCAAAAACGGCCATGACCATCGTGCTTGGTGCTGCGCTGAAGATTGTGATATATTTGCACTTGGCGCAGCTTTGGAGGCTTTTTAA

Protein sequence

>Msa0423200

MDPSHVSSYVNFSCELRIIQARNIEFIKTTKNLFARLYLPTENNKRIQLNSKNVSAKSLPFWDESFSLDCSCPQDFMENLNQQSLVLELRQRKIWGSKLIGKGEIPWKVILESQNMELKKWLKMDLVSRSDCKEGMFITPEVEVEIKIKVASVAEMEKQNKRRMNNLNECGCKNGHDHRAWCCAEDCDIFALGAALEAF*