AlfalfaGEDB | Msa0862800

M. sativa cultivar zhongmu-4 / Msa0862800

KEGG: K00658
GO: -
PFAM: PF00198.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0862800	XP_003593015.1	88.000	225	1	1	1	199	229	453	8.59e-134	392

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0862800	sp\|Q8H107\|ODO2B_ARATH	76.000	225	28	1	1	199	240	464	1.67e-120	352

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0862800	G7IPF6	88.000	225	1	1	1	199	229	453	4.10e-134	392

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0024210	Msa0862800	0.801183	1.015219e-48	-8.615850e-47
Msa0063350	Msa0862800	0.809754	1.618365e-50	-8.615850e-47
Msa0120010	Msa0862800	0.807726	4.390266e-50	-8.615850e-47
Msa0120020	Msa0862800	0.816592	5.108929e-52	-8.615850e-47
Msa0140510	Msa0862800	0.830637	2.625164e-55	-8.615850e-47
Msa0140520	Msa0862800	0.816806	4.574326e-52	-8.615850e-47
Msa0178860	Msa0862800	0.809455	1.875872e-50	-8.615850e-47
Msa0183010	Msa0862800	0.807597	4.677814e-50	-8.615850e-47
Msa0230640	Msa0862800	0.808251	3.394143e-50	-8.615850e-47
Msa0279210	Msa0862800	0.839880	1.215839e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0862800	MtrunA17_Chr2g0277631	88.000	225	1	1	1	199	229	453	7.90e-138	392
Msa0862800	MtrunA17_Chr5g0444531	87.556	225	2	1	1	199	231	455	1.52e-137	392
Msa0862800	MtrunA17_Chr1g0197901	29.870	231	121	5	2	197	401	625	3.60e-22	93.6
Msa0862800	MtrunA17_Chr8g0343061	30.702	228	125	5	1	197	318	543	9.37e-21	89.4
Msa0862800	MtrunA17_Chr4g0067561	34.351	131	85	1	70	199	389	519	5.05e-19	84.3
Msa0862800	MtrunA17_Chr1g0200441	32.283	127	81	2	72	195	331	455	9.51e-14	68.9
Msa0862800	MtrunA17_Chr1g0210021	32.558	129	82	2	72	197	344	470	1.02e-12	65.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0862800	AT4G26910.3	76.000	225	28	1	1	199	141	365	2.17e-122	350
Msa0862800	AT4G26910.2	76.000	225	28	1	1	199	239	463	1.43e-121	352
Msa0862800	AT4G26910.1	76.000	225	28	1	1	199	240	464	1.70e-121	352
Msa0862800	AT5G55070.1	76.000	225	28	1	1	199	240	464	1.54e-120	349
Msa0862800	AT5G55070.2	76.000	225	28	1	1	199	240	464	1.54e-120	349
Msa0862800	AT3G52200.2	31.304	230	119	5	2	197	489	713	2.76e-23	97.1
Msa0862800	AT3G52200.1	31.304	230	119	5	2	197	413	637	2.96e-23	97.1
Msa0862800	AT3G13930.1	31.739	230	118	7	2	197	315	539	1.36e-20	89.4
Msa0862800	AT1G54220.1	29.825	228	125	6	2	197	315	539	1.43e-20	89.0
Msa0862800	AT1G54220.2	29.825	228	125	6	2	197	315	539	1.43e-20	89.0

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Find 124 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTCAAGGCCCAAACAAA+AGG	0.444465	5_4:+62541483	None:intergenic
AACAACTACGATGCATATTA+TGG	0.197684	5_4:-62541319	Msa0862800:three_prime_UTR
AACATTTCCTCCAACAACCG+CGG	0.602311	5_4:+62541701	None:intergenic
AACTTTATCAATTGATGAGA+TGG	0.507014	5_4:-62542245	Msa0862800:CDS
AAGAGGCTTCCATTCAAACA+TGG	0.648188	5_4:-62545345	Msa0862800:five_prime_UTR
AAGCATCCTTATAATCAGCA+CGG	0.676021	5_4:+62542978	None:intergenic
AAGCCTGCGAGGGTCCTCCA+CGG	0.659181	5_4:+62541584	None:intergenic
AATGTAACGAAACCATGGCA+TGG	0.600925	5_4:-62541169	Msa0862800:three_prime_UTR
AATGTCCCTGCATAGTAATA+AGG	0.425481	5_4:+62545375	None:intergenic
AATTCAAGGCCCAAACAAAA+GGG	0.563782	5_4:+62541484	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGATAACCTTATTACTATGC+AGG	-	chr5_4:62541228-62541247	Msa0862800:three_prime_UTR	30.0%
GATAACCTTATTACTATGCA+GGG	-	chr5_4:62541229-62541248	Msa0862800:three_prime_UTR	30.0%
TATAGAAGAATGACAATCCT+TGG	-	chr5_4:62541589-62541608	Msa0862800:CDS	30.0%
ATAGAAGAATGACAATCCTT+GGG	-	chr5_4:62541590-62541609	Msa0862800:CDS	30.0%
GTAATGTTGTTAAGTTAAGC+AGG	+	chr5_4:62541661-62541680	None:intergenic	30.0%
TTAGCAATCACAGGAAAATT+TGG	+	chr5_4:62541780-62541799	None:intergenic	30.0%
TCTGTTTCAATTTGAGCAAT+TGG	+	chr5_4:62542109-62542128	None:intergenic	30.0%
TGCTCAAATTGAAACAGATA+AGG	-	chr5_4:62542111-62542130	Msa0862800:intron	30.0%
TATTTGGGAACAAAGAAAAC+TGG	+	chr5_4:62542197-62542216	None:intergenic	30.0%
ATCTATTAAACAGTACCTTC+AGG	+	chr5_4:62542292-62542311	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	62541148	62545483	62541148	ID=Msa0862800;Name=Msa0862800
chr5_4	mRNA	62541148	62545483	62541148	ID=Msa0862800-mRNA-1;Parent=Msa0862800;Name=Msa0862800-mRNA-1;_AED=0.12;_eAED=0.12;_QI=556\|0.77\|0.7\|1\|0.77\|0.7\|10\|421\|199
chr5_4	exon	62541148	62541760	62541148	ID=Msa0862800-mRNA-1:exon:19542;Parent=Msa0862800-mRNA-1
chr5_4	exon	62542168	62542362	62542168	ID=Msa0862800-mRNA-1:exon:19541;Parent=Msa0862800-mRNA-1
chr5_4	exon	62542805	62542837	62542805	ID=Msa0862800-mRNA-1:exon:19540;Parent=Msa0862800-mRNA-1
chr5_4	exon	62542928	62543018	62542928	ID=Msa0862800-mRNA-1:exon:19539;Parent=Msa0862800-mRNA-1
chr5_4	exon	62543787	62543881	62543787	ID=Msa0862800-mRNA-1:exon:19538;Parent=Msa0862800-mRNA-1
chr5_4	exon	62543963	62544186	62543963	ID=Msa0862800-mRNA-1:exon:19537;Parent=Msa0862800-mRNA-1
chr5_4	exon	62544350	62544380	62544350	ID=Msa0862800-mRNA-1:exon:19536;Parent=Msa0862800-mRNA-1
chr5_4	exon	62544499	62544554	62544499	ID=Msa0862800-mRNA-1:exon:19535;Parent=Msa0862800-mRNA-1
chr5_4	exon	62544659	62544733	62544659	ID=Msa0862800-mRNA-1:exon:19534;Parent=Msa0862800-mRNA-1
chr5_4	exon	62545320	62545483	62545320	ID=Msa0862800-mRNA-1:exon:19533;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62545320	62545483	62545320	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62544659	62544733	62544659	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62544499	62544554	62544499	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62544350	62544380	62544350	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62543963	62544186	62543963	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	five_prime_UTR	62543876	62543881	62543876	ID=Msa0862800-mRNA-1:five_prime_utr;Parent=Msa0862800-mRNA-1
chr5_4	CDS	62543787	62543875	62543787	ID=Msa0862800-mRNA-1:cds;Parent=Msa0862800-mRNA-1
chr5_4	CDS	62542928	62543018	62542928	ID=Msa0862800-mRNA-1:cds;Parent=Msa0862800-mRNA-1
chr5_4	CDS	62542805	62542837	62542805	ID=Msa0862800-mRNA-1:cds;Parent=Msa0862800-mRNA-1
chr5_4	CDS	62542168	62542362	62542168	ID=Msa0862800-mRNA-1:cds;Parent=Msa0862800-mRNA-1
chr5_4	CDS	62541569	62541760	62541569	ID=Msa0862800-mRNA-1:cds;Parent=Msa0862800-mRNA-1
chr5_4	three_prime_UTR	62541148	62541568	62541148	ID=Msa0862800-mRNA-1:three_prime_utr;Parent=Msa0862800-mRNA-1

Gene Sequence

>Msa0862800

ATGACAAGGCTTCGGAAACGGGTTGCTCAACGGTTGAAGGATTCCCAAAACACATTTGCTATGCTGACAACCTTCAATGAAGTTGATATGACTAATTTGATGAAGCTCCGTGCTGATTATAAGGATGCTTTCGTTGAAAAGCATGGAGTCAAATTGGGACTTATGTCTGGGTTTATCAAAGCTGCTGTCAATGCACTTCAATATCAACCAATTGGACTTGTTGTGCCTGTCATCCGAAATGCGGATAAAATGAACTTTGCGGATATAGAAAAACAGATCAATGTTTTTGCAAAGAAGGCTAATGCTGGAACTTTATCAATTGATGAGATGGCCGGAGGCACTCTTACAATATCAAATGGTGGTGTTTATGGCAGTCTTTTGAGTACCCCTATTATCAACCCACCTCAGTCGGCAATCTTGGGTATGCATTCAATTGTGAGCCGTCCCGCGGTTGTTGGAGGAAATGTTGTTCCAAGGCCACTGATGTACATTGCTCTAACATATGACCATAGAATCATTGATGGAAGAGAGGCAGTGTTCTTTTTGAGACGGATCAAAGATACCGTGGAGGACCCTCGCAGGCTTTTGCTCGACATATAA

Protein sequence

>Msa0862800

MTRLRKRVAQRLKDSQNTFAMLTTFNEVDMTNLMKLRADYKDAFVEKHGVKLGLMSGFIKAAVNALQYQPIGLVVPVIRNADKMNFADIEKQINVFAKKANAGTLSIDEMAGGTLTISNGGVYGSLLSTPIINPPQSAILGMHSIVSRPAVVGGNVVPRPLMYIALTYDHRIIDGREAVFFLRRIKDTVEDPRRLLLDI*