Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016570 | Msa0952350 | 0.800377 | 1.483289e-48 | -8.615850e-47 |
| Msa0016580 | Msa0952350 | 0.815653 | 8.281325e-52 | -8.615850e-47 |
| Msa0102070 | Msa0952350 | 0.812737 | 3.646545e-51 | -8.615850e-47 |
| Msa0110270 | Msa0952350 | 0.809309 | 2.016365e-50 | -8.615850e-47 |
| Msa0726140 | Msa0952350 | 0.843516 | 1.335729e-58 | -8.615850e-47 |
| Msa0729800 | Msa0952350 | 0.846064 | 2.745454e-59 | -8.615850e-47 |
| Msa0733290 | Msa0952350 | 0.834656 | 2.641966e-56 | -8.615850e-47 |
| Msa0743810 | Msa0952350 | 0.826872 | 2.136706e-54 | -8.615850e-47 |
| Msa0745060 | Msa0952350 | 0.827778 | 1.296684e-54 | -8.615850e-47 |
| Msa0783160 | Msa0952350 | 0.845382 | 4.204954e-59 | -8.615850e-47 |
| Msa0787600 | Msa0952350 | 0.806632 | 7.484161e-50 | -8.615850e-47 |
| Msa0808010 | Msa0952350 | 0.837269 | 5.742342e-57 | -8.615850e-47 |
| Msa0808020 | Msa0952350 | 0.833299 | 5.776895e-56 | -8.615850e-47 |
| Msa0825860 | Msa0952350 | 0.836101 | 1.139942e-56 | -8.615850e-47 |
| Msa0200000 | Msa0952350 | 0.812073 | 5.093490e-51 | -8.615850e-47 |
| Msa0227480 | Msa0952350 | 0.828543 | 8.479731e-55 | -8.615850e-47 |
| Msa0232850 | Msa0952350 | 0.810612 | 1.056965e-50 | -8.615850e-47 |
| Msa0365500 | Msa0952350 | 0.803490 | 3.401059e-49 | -8.615850e-47 |
| Msa0384800 | Msa0952350 | 0.805837 | 1.100831e-49 | -8.615850e-47 |
| Msa0384890 | Msa0952350 | 0.805804 | 1.118540e-49 | -8.615850e-47 |
| Msa0414410 | Msa0952350 | 0.815344 | 9.701486e-52 | -8.615850e-47 |
| Msa0414420 | Msa0952350 | 0.846668 | 1.879719e-59 | -8.615850e-47 |
| Msa0450000 | Msa0952350 | 0.828305 | 9.681824e-55 | -8.615850e-47 |
| Msa0452900 | Msa0952350 | 0.850335 | 1.814518e-60 | -8.615850e-47 |
| Msa0267000 | Msa0952350 | 0.814700 | 1.348072e-51 | -8.615850e-47 |
| Msa0267010 | Msa0952350 | 0.821219 | 4.535057e-53 | -8.615850e-47 |
| Msa0342240 | Msa0952350 | 0.824226 | 9.052078e-54 | -8.615850e-47 |
| Msa0491590 | Msa0952350 | 0.810634 | 1.045350e-50 | -8.615850e-47 |
| Msa0534540 | Msa0952350 | 0.812673 | 3.766257e-51 | -8.615850e-47 |
| Msa0564890 | Msa0952350 | 0.823808 | 1.134773e-53 | -8.615850e-47 |
| Msa0596150 | Msa0952350 | 0.809838 | 1.552333e-50 | -8.615850e-47 |
| Msa0846210 | Msa0952350 | 0.808611 | 2.844077e-50 | -8.615850e-47 |
| Msa0846330 | Msa0952350 | 0.831388 | 1.717461e-55 | -8.615850e-47 |
| Msa0846350 | Msa0952350 | 0.854425 | 1.241503e-61 | -8.615850e-47 |
| Msa0848960 | Msa0952350 | 0.823927 | 1.063986e-53 | -8.615850e-47 |
| Msa0858980 | Msa0952350 | 0.815659 | 8.256101e-52 | -8.615850e-47 |
| Msa0865320 | Msa0952350 | 0.816508 | 5.335191e-52 | -8.615850e-47 |
| Msa0888370 | Msa0952350 | 0.811749 | 5.991240e-51 | -8.615850e-47 |
| Msa0896080 | Msa0952350 | 0.817823 | 2.700739e-52 | -8.615850e-47 |
| Msa0914280 | Msa0952350 | 0.805140 | 1.541049e-49 | -8.615850e-47 |
| Msa0952350 | Msa0989060 | 0.802184 | 6.327114e-49 | -8.615850e-47 |
| Msa0952350 | Msa1016280 | 0.825186 | 5.378148e-54 | -8.615850e-47 |
| Msa0952350 | Msa1025360 | 0.827985 | 1.155687e-54 | -8.615850e-47 |
| Msa0952350 | Msa1025370 | 0.813332 | 2.700386e-51 | -8.615850e-47 |
| Msa0952350 | Msa1044830 | 0.816897 | 4.364034e-52 | -8.615850e-47 |
| Msa0952350 | Msa1044840 | 0.829789 | 4.228305e-55 | -8.615850e-47 |
| Msa0952350 | Msa1092990 | 0.825688 | 4.090208e-54 | -8.615850e-47 |
| Msa0952350 | Msa1100980 | 0.832470 | 9.280124e-56 | -8.615850e-47 |
| Msa0952350 | Msa1109440 | 0.802795 | 4.734180e-49 | -8.615850e-47 |
| Msa0952350 | Msa1121200 | 0.810980 | 8.801845e-51 | -8.615850e-47 |
| Msa0952350 | Msa1135740 | 0.809401 | 1.926789e-50 | -8.615850e-47 |
| Msa0952350 | Msa1146110 | 0.807912 | 4.008957e-50 | -8.615850e-47 |
| Msa0952350 | Msa1192640 | 0.814657 | 1.377618e-51 | -8.615850e-47 |
| Msa0952350 | Msa1269670 | 0.827906 | 1.207377e-54 | -8.615850e-47 |
| Msa0952350 | Msa1270230 | 0.829238 | 5.756485e-55 | -8.615850e-47 |
| Msa0952350 | Msa1270240 | 0.812975 | 3.235028e-51 | -8.615850e-47 |
| Msa0952350 | Msa1270270 | 0.823042 | 1.713421e-53 | -8.615850e-47 |
| Msa0952350 | Msa1283330 | 0.837119 | 6.271628e-57 | -8.615850e-47 |
| Msa0952350 | Msa1285930 | 0.822673 | 2.089169e-53 | -8.615850e-47 |
| Msa0952350 | Msa1287800 | 0.823308 | 1.485170e-53 | -8.615850e-47 |
| Msa0952350 | Msa1287810 | 0.837178 | 6.060193e-57 | -8.615850e-47 |
| Msa0952350 | Msa1320300 | 0.818757 | 1.659076e-52 | -8.615850e-47 |
| Msa0952350 | Msa1340590 | 0.836969 | 6.853123e-57 | -8.615850e-47 |
| Msa0952350 | Msa1397580 | 0.831661 | 1.470931e-55 | -8.615850e-47 |
| Msa0952350 | Msa1404000 | 0.832552 | 8.855373e-56 | -8.615850e-47 |
| Msa0952350 | Msa1415310 | 0.823696 | 1.205478e-53 | -8.615850e-47 |
| Msa0952350 | Msa1422180 | 0.812483 | 4.144432e-51 | -8.615850e-47 |
| Msa0952350 | Msa1433120 | 0.815100 | 1.099114e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0952350 | MtrunA17_Chr6g0469541 | 100.000 | 30 | 0 | 0 | 1 | 30 | 20 | 49 | 2.46e-14 | 60.1 |
| Msa0952350 | MtrunA17_Chr7g0233181 | 83.333 | 30 | 5 | 0 | 1 | 30 | 20 | 49 | 4.30e-12 | 54.3 |
| Msa0952350 | MtrunA17_Chr7g0233251 | 80.000 | 30 | 6 | 0 | 1 | 30 | 20 | 49 | 6.43e-11 | 51.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGACAGTGTCCAGCCTTTC+AGG | 0.233115 | 6_3:-46573290 | Msa0952350:intron |
| GAGAAGAAACTGACGTTAAC+AGG | 0.282306 | 6_3:-46572699 | Msa0952350:CDS |
| ACAAACCTCAACAATAATAA+AGG | 0.376511 | 6_3:-46573545 | Msa0952350:intron |
| TTTATTATTGTTGAGGTTTG+TGG | 0.386086 | 6_3:+46573547 | None:intergenic |
| TTAACAGAAAGTAGTGTTGA+AGG | 0.397592 | 6_3:-46573351 | Msa0952350:intron |
| TTATTATTGTTGAGGTTTGT+GGG | 0.419362 | 6_3:+46573548 | None:intergenic |
| ATATATATTACCTGAAAGGC+TGG | 0.445809 | 6_3:+46573280 | None:intergenic |
| ATTATTGATGCCAAATGTAA+AGG | 0.458445 | 6_3:+46573583 | None:intergenic |
| TAATGATAGAACCAAGCAAA+AGG | 0.516485 | 6_3:-46573318 | Msa0952350:five_prime_UTR |
| AATGTAAAGGATTTCAAAAG+AGG | 0.521012 | 6_3:+46573596 | None:intergenic |
| TTTAATATATATTACCTGAA+AGG | 0.535056 | 6_3:+46573276 | None:intergenic |
| AGAAGAAACTGACGTTAACA+GGG | 0.594314 | 6_3:-46572698 | Msa0952350:CDS |
| GAAGAAACTGACGTTAACAG+GGG | 0.758236 | 6_3:-46572697 | Msa0952350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATATATATTACCTGAA+AGG | + | chr6_3:46573055-46573074 | None:intergenic | 15.0% |
| !! | ATTTACGAATTATTTCAAAT+AGG | - | chr6_3:46573542-46573561 | Msa0952350:intron | 15.0% |
| !!! | TTTTTTTTTATCAATACTGT+AGG | - | chr6_3:46573578-46573597 | Msa0952350:five_prime_UTR | 15.0% |
| !!! | TTTTTTTTATCAATACTGTA+GGG | - | chr6_3:46573579-46573598 | Msa0952350:five_prime_UTR | 15.0% |
| !! | TTTAATATATATTACCTGAA+AGG | + | chr6_3:46573055-46573074 | None:intergenic | 15.0% |
| !! | ATTTACGAATTATTTCAAAT+AGG | - | chr6_3:46573542-46573561 | Msa0952350:intron | 15.0% |
| !!! | TTTTTTTTTATCAATACTGT+AGG | - | chr6_3:46573578-46573597 | Msa0952350:five_prime_UTR | 15.0% |
| !!! | TTTTTTTTATCAATACTGTA+GGG | - | chr6_3:46573579-46573598 | Msa0952350:five_prime_UTR | 15.0% |
| !!! | TTTTACCTTTATTATTGTTG+AGG | + | chr6_3:46572791-46572810 | None:intergenic | 20.0% |
| !! | AAAAAATAGGTTTCATAACT+AGG | + | chr6_3:46573282-46573301 | None:intergenic | 20.0% |
| !! | TTTAACATATCAAATGTTCT+CGG | - | chr6_3:46573312-46573331 | Msa0952350:exon | 20.0% |
| !!! | TTCTAGCATTTTTTTTAAAG+AGG | + | chr6_3:46573498-46573517 | None:intergenic | 20.0% |
| !!! | TTTTTTTATCAATACTGTAG+GGG | - | chr6_3:46573580-46573599 | Msa0952350:five_prime_UTR | 20.0% |
| !!! | TTTTACCTTTATTATTGTTG+AGG | + | chr6_3:46572791-46572810 | None:intergenic | 20.0% |
| !! | AAAAAATAGGTTTCATAACT+AGG | + | chr6_3:46573282-46573301 | None:intergenic | 20.0% |
| !! | TTTAACATATCAAATGTTCT+CGG | - | chr6_3:46573312-46573331 | Msa0952350:exon | 20.0% |
| !!! | TTCTAGCATTTTTTTTAAAG+AGG | + | chr6_3:46573498-46573517 | None:intergenic | 20.0% |
| !!! | TTTTTTTATCAATACTGTAG+GGG | - | chr6_3:46573580-46573599 | Msa0952350:five_prime_UTR | 20.0% |
| ! | AATGTAAAGGATTTCAAAAG+AGG | + | chr6_3:46572735-46572754 | None:intergenic | 25.0% |
| !!! | CTTTTGAAATCCTTTACATT+TGG | - | chr6_3:46572735-46572754 | Msa0952350:intron | 25.0% |
| ! | ATTATTGATGCCAAATGTAA+AGG | + | chr6_3:46572748-46572767 | None:intergenic | 25.0% |
| ! | TTATTATTGTTGAGGTTTGT+GGG | + | chr6_3:46572783-46572802 | None:intergenic | 25.0% |
| ! | TTTATTATTGTTGAGGTTTG+TGG | + | chr6_3:46572784-46572803 | None:intergenic | 25.0% |
| ! | ACAAACCTCAACAATAATAA+AGG | - | chr6_3:46572783-46572802 | Msa0952350:intron | 25.0% |
| ! | AAATGAGAAAAGTGAGAAAA+TGG | + | chr6_3:46572830-46572849 | None:intergenic | 25.0% |
| ! | AAATCACGTGATCAAAAAAT+AGG | + | chr6_3:46573295-46573314 | None:intergenic | 25.0% |
| !! | TTTGAGTGAATTGTTTTCTA+TGG | + | chr6_3:46572686-46572705 | None:intergenic | 25.0% |
| ! | AATGTAAAGGATTTCAAAAG+AGG | + | chr6_3:46572735-46572754 | None:intergenic | 25.0% |
| !!! | CTTTTGAAATCCTTTACATT+TGG | - | chr6_3:46572735-46572754 | Msa0952350:intron | 25.0% |
| ! | ATTATTGATGCCAAATGTAA+AGG | + | chr6_3:46572748-46572767 | None:intergenic | 25.0% |
| ! | TTATTATTGTTGAGGTTTGT+GGG | + | chr6_3:46572783-46572802 | None:intergenic | 25.0% |
| ! | TTTATTATTGTTGAGGTTTG+TGG | + | chr6_3:46572784-46572803 | None:intergenic | 25.0% |
| ! | ACAAACCTCAACAATAATAA+AGG | - | chr6_3:46572783-46572802 | Msa0952350:intron | 25.0% |
| ! | AAATGAGAAAAGTGAGAAAA+TGG | + | chr6_3:46572830-46572849 | None:intergenic | 25.0% |
| ! | AAATCACGTGATCAAAAAAT+AGG | + | chr6_3:46573295-46573314 | None:intergenic | 25.0% |
| ! | TTAACAGAAAGTAGTGTTGA+AGG | - | chr6_3:46572977-46572996 | Msa0952350:intron | 30.0% |
| TAATGATAGAACCAAGCAAA+AGG | - | chr6_3:46573010-46573029 | Msa0952350:intron | 30.0% | |
| ATATATATTACCTGAAAGGC+TGG | + | chr6_3:46573051-46573070 | None:intergenic | 30.0% | |
| AGTACTACTACAATGTAATG+TGG | - | chr6_3:46573091-46573110 | Msa0952350:intron | 30.0% | |
| !!! | ATGTGGACTTTTTTTTTGAG+AGG | - | chr6_3:46573108-46573127 | Msa0952350:intron | 30.0% |
| !!! | TGTGGACTTTTTTTTTGAGA+GGG | - | chr6_3:46573109-46573128 | Msa0952350:intron | 30.0% |
| ! | TTAACAGAAAGTAGTGTTGA+AGG | - | chr6_3:46572977-46572996 | Msa0952350:intron | 30.0% |
| TAATGATAGAACCAAGCAAA+AGG | - | chr6_3:46573010-46573029 | Msa0952350:intron | 30.0% | |
| ATATATATTACCTGAAAGGC+TGG | + | chr6_3:46573051-46573070 | None:intergenic | 30.0% | |
| AGTACTACTACAATGTAATG+TGG | - | chr6_3:46573091-46573110 | Msa0952350:intron | 30.0% | |
| !!! | ATGTGGACTTTTTTTTTGAG+AGG | - | chr6_3:46573108-46573127 | Msa0952350:intron | 30.0% |
| !!! | TGTGGACTTTTTTTTTGAGA+GGG | - | chr6_3:46573109-46573128 | Msa0952350:intron | 30.0% |
| ! | TGTCTTCATAACCTTTTGCT+TGG | + | chr6_3:46573024-46573043 | None:intergenic | 35.0% |
| !! | TTTTTGAGAGGGATGTAATG+TGG | - | chr6_3:46573120-46573139 | Msa0952350:intron | 35.0% |
| AGAAGAAACTGACGTTAACA+GGG | - | chr6_3:46573630-46573649 | Msa0952350:five_prime_UTR | 35.0% | |
| ! | TGTCTTCATAACCTTTTGCT+TGG | + | chr6_3:46573024-46573043 | None:intergenic | 35.0% |
| !! | TTTTTGAGAGGGATGTAATG+TGG | - | chr6_3:46573120-46573139 | Msa0952350:intron | 35.0% |
| AGAAGAAACTGACGTTAACA+GGG | - | chr6_3:46573630-46573649 | Msa0952350:five_prime_UTR | 35.0% | |
| GAGAAGAAACTGACGTTAAC+AGG | - | chr6_3:46573629-46573648 | Msa0952350:five_prime_UTR | 40.0% | |
| GAAGAAACTGACGTTAACAG+GGG | - | chr6_3:46573631-46573650 | Msa0952350:five_prime_UTR | 40.0% | |
| GAGAAGAAACTGACGTTAAC+AGG | - | chr6_3:46573629-46573648 | Msa0952350:five_prime_UTR | 40.0% | |
| GAAGAAACTGACGTTAACAG+GGG | - | chr6_3:46573631-46573650 | Msa0952350:five_prime_UTR | 40.0% | |
| !! | TTTAATAATAGATATAATTA+TGG | - | chr6_3:46572921-46572940 | Msa0952350:intron | 5.0% |
| !! | TTAATAATAGATATAATTAT+GGG | - | chr6_3:46572922-46572941 | Msa0952350:intron | 5.0% |
| !! | TTTAATAATAGATATAATTA+TGG | - | chr6_3:46572921-46572940 | Msa0952350:intron | 5.0% |
| !! | TTAATAATAGATATAATTAT+GGG | - | chr6_3:46572922-46572941 | Msa0952350:intron | 5.0% |
| AAGACAGTGTCCAGCCTTTC+AGG | - | chr6_3:46573038-46573057 | Msa0952350:intron | 50.0% | |
| AAGACAGTGTCCAGCCTTTC+AGG | - | chr6_3:46573038-46573057 | Msa0952350:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 46572686 | 46573664 | 46572686 | ID=Msa0952350;Name=Msa0952350 |
| chr6_3 | mRNA | 46572686 | 46573664 | 46572686 | ID=Msa0952350-mRNA-1;Parent=Msa0952350;Name=Msa0952350-mRNA-1;_AED=0.23;_eAED=0.23;_QI=170|0.5|0.33|1|0.5|0.33|3|0|30 |
| chr6_3 | exon | 46572686 | 46572750 | 46572686 | ID=Msa0952350-mRNA-1:exon:10294;Parent=Msa0952350-mRNA-1 |
| chr6_3 | exon | 46573291 | 46573366 | 46573291 | ID=Msa0952350-mRNA-1:exon:10293;Parent=Msa0952350-mRNA-1 |
| chr6_3 | exon | 46573546 | 46573664 | 46573546 | ID=Msa0952350-mRNA-1:exon:10292;Parent=Msa0952350-mRNA-1 |
| chr6_3 | five_prime_UTR | 46573546 | 46573664 | 46573546 | ID=Msa0952350-mRNA-1:five_prime_utr;Parent=Msa0952350-mRNA-1 |
| chr6_3 | five_prime_UTR | 46573316 | 46573366 | 46573316 | ID=Msa0952350-mRNA-1:five_prime_utr;Parent=Msa0952350-mRNA-1 |
| chr6_3 | CDS | 46573291 | 46573315 | 46573291 | ID=Msa0952350-mRNA-1:cds;Parent=Msa0952350-mRNA-1 |
| chr6_3 | CDS | 46572686 | 46572750 | 46572686 | ID=Msa0952350-mRNA-1:cds;Parent=Msa0952350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
