AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0896080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0896080 MtrunA17_Chr6g0482501 90.909 55 5 0 17 71 477 531 2.92e-27 102
Msa0896080 MtrunA17_Chr6g0482481 85.185 54 8 0 17 70 134 187 6.65e-26 93.6
Msa0896080 MtrunA17_Chr6g0482371 70.968 62 18 0 10 71 62 123 2.81e-25 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAACAGATTGAGATTT+TGG 0.155235 6_1:-85579777 Msa0896080:CDS
GTTCTTCAGCTTCTCCTATT+GGG 0.285656 6_1:+85579792 None:intergenic
TGTTCTTCAGCTTCTCCTAT+TGG 0.317888 6_1:+85579791 None:intergenic
TGTCAAGAATGCGTTAGATT+TGG 0.348270 6_1:-85579412 Msa0896080:CDS
TGCAAGAAGTCGGTATCTTC+TGG 0.350045 6_1:-85579731 Msa0896080:CDS
AATATCTTGGCTGGCGCATA+AGG 0.372722 6_1:-85579237 Msa0896080:three_prime_UTR
CAGCATTGCTTGGTAATTGT+TGG 0.423362 6_1:-85579307 Msa0896080:three_prime_UTR
TGGAAAAGACTGTGCCAGAC+TGG 0.439177 6_1:-85579757 Msa0896080:CDS
ATACATCTTGTCCTCCCAAT+AGG 0.445957 6_1:-85579806 Msa0896080:CDS
GACTTCTTGCAAAGCCAGTC+TGG 0.462523 6_1:+85579743 None:intergenic
AATTGTTGCTCAGCATTGCT+TGG 0.475440 6_1:-85579317 Msa0896080:three_prime_UTR
ACCAGCTATAGTGAATATCT+TGG 0.485587 6_1:-85579250 Msa0896080:three_prime_UTR
GCATAAGGGATTGTAAGAAC+TGG 0.491937 6_1:-85579222 Msa0896080:three_prime_UTR
GCCAAGATATTCACTATAGC+TGG 0.499828 6_1:+85579249 None:intergenic
AGAGAAGTGGAGTGAGAGAG+TGG 0.541048 6_1:+85579917 None:intergenic
GGTATAGAAGACAAGAGAAG+TGG 0.541866 6_1:+85579904 None:intergenic
CAGACAGAGAATACAATATG+TGG 0.551554 6_1:+85579169 None:intergenic
CTTCTGGAGACACTATGTAC+TGG 0.555300 6_1:-85579715 Msa0896080:intron
CTTCAGCTTCTCCTATTGGG+AGG 0.555591 6_1:+85579795 None:intergenic
GCTATAGTGAATATCTTGGC+TGG 0.556676 6_1:-85579246 Msa0896080:three_prime_UTR
ATATCTTGGCTGGCGCATAA+GGG 0.560983 6_1:-85579236 Msa0896080:three_prime_UTR
ATTGCTTGGTAATTGTTGGT+TGG 0.572533 6_1:-85579303 Msa0896080:three_prime_UTR
CAACAACGAAGATGAGTGAA+AGG 0.610775 6_1:-85579358 Msa0896080:exon
AGACTGGCTTTGCAAGAAGT+CGG 0.629242 6_1:-85579741 Msa0896080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAAATTTTACATATATCA+TGG - chr6_1:85579514-85579533 Msa0896080:intron 15.0%
!!! ACAAAATTTTACATATATCA+TGG - chr6_1:85579514-85579533 Msa0896080:intron 15.0%
!!! TTTAGGTTTGAAATTTTGTT+TGG + chr6_1:85579223-85579242 None:intergenic 20.0%
!! TACATATATCATGGATATAA+TGG - chr6_1:85579523-85579542 Msa0896080:intron 20.0%
!!! TAAATTGTTCTTAAGCTTTT+TGG - chr6_1:85579886-85579905 Msa0896080:intron 20.0%
!!! TTTAGGTTTGAAATTTTGTT+TGG + chr6_1:85579223-85579242 None:intergenic 20.0%
!! TACATATATCATGGATATAA+TGG - chr6_1:85579523-85579542 Msa0896080:intron 20.0%
!!! TAAATTGTTCTTAAGCTTTT+TGG - chr6_1:85579886-85579905 Msa0896080:intron 20.0%
!!! GAAATTTTGTTTGGTAGTAT+GGG + chr6_1:85579214-85579233 None:intergenic 25.0%
!!! TGAAATTTTGTTTGGTAGTA+TGG + chr6_1:85579215-85579234 None:intergenic 25.0%
!!! TGGAATTTCATGTTTTAAGT+TGG + chr6_1:85579472-85579491 None:intergenic 25.0%
! AGCAGAACTTTAAATGTTAT+TGG - chr6_1:85579549-85579568 Msa0896080:intron 25.0%
!!! GAAATTTTGTTTGGTAGTAT+GGG + chr6_1:85579214-85579233 None:intergenic 25.0%
!!! TGAAATTTTGTTTGGTAGTA+TGG + chr6_1:85579215-85579234 None:intergenic 25.0%
!!! TGGAATTTCATGTTTTAAGT+TGG + chr6_1:85579472-85579491 None:intergenic 25.0%
! AGCAGAACTTTAAATGTTAT+TGG - chr6_1:85579549-85579568 Msa0896080:intron 25.0%
! CAATTAGCAGTTATGGTTTT+AGG + chr6_1:85579240-85579259 None:intergenic 30.0%
CTAAAACCATAACTGCTAAT+TGG - chr6_1:85579238-85579257 Msa0896080:three_prime_UTR 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85579303-85579322 Msa0896080:three_prime_UTR 30.0%
! CTGATACTCTAAAAGAACTA+AGG + chr6_1:85579421-85579440 None:intergenic 30.0%
!! TTAGAGTATCAGTCTCTTTT+AGG - chr6_1:85579429-85579448 Msa0896080:intron 30.0%
!! TAGAGTATCAGTCTCTTTTA+GGG - chr6_1:85579430-85579449 Msa0896080:intron 30.0%
!!! GTTTTTTTATAGCCCAAAGA+AGG + chr6_1:85579496-85579515 None:intergenic 30.0%
! CAATTAGCAGTTATGGTTTT+AGG + chr6_1:85579240-85579259 None:intergenic 30.0%
CTAAAACCATAACTGCTAAT+TGG - chr6_1:85579238-85579257 Msa0896080:three_prime_UTR 30.0%
! TGAAGAACAGATTGAGATTT+TGG - chr6_1:85579303-85579322 Msa0896080:three_prime_UTR 30.0%
! CTGATACTCTAAAAGAACTA+AGG + chr6_1:85579421-85579440 None:intergenic 30.0%
!! TTAGAGTATCAGTCTCTTTT+AGG - chr6_1:85579429-85579448 Msa0896080:intron 30.0%
!! TAGAGTATCAGTCTCTTTTA+GGG - chr6_1:85579430-85579449 Msa0896080:intron 30.0%
!!! GTTTTTTTATAGCCCAAAGA+AGG + chr6_1:85579496-85579515 None:intergenic 30.0%
! TTTGTTTGGTAGTATGGGTT+TGG + chr6_1:85579209-85579228 None:intergenic 35.0%
ACACAACCAATTAGCAGTTA+TGG + chr6_1:85579247-85579266 None:intergenic 35.0%
ATGAAATTCCAGCCTTCTTT+GGG - chr6_1:85579481-85579500 Msa0896080:intron 35.0%
! TATTGGTAGCAAAAATCCCT+TGG - chr6_1:85579566-85579585 Msa0896080:intron 35.0%
CTTGACACTGCAAATATAAC+AGG + chr6_1:85579655-85579674 None:intergenic 35.0%
TGTCAAGAATGCGTTAGATT+TGG - chr6_1:85579668-85579687 Msa0896080:intron 35.0%
! ATTGCTTGGTAATTGTTGGT+TGG - chr6_1:85579777-85579796 Msa0896080:CDS 35.0%
ACCAGCTATAGTGAATATCT+TGG - chr6_1:85579830-85579849 Msa0896080:intron 35.0%
CAGACAGAGAATACAATATG+TGG + chr6_1:85579914-85579933 None:intergenic 35.0%
! TTTGTTTGGTAGTATGGGTT+TGG + chr6_1:85579209-85579228 None:intergenic 35.0%
ACACAACCAATTAGCAGTTA+TGG + chr6_1:85579247-85579266 None:intergenic 35.0%
ATGAAATTCCAGCCTTCTTT+GGG - chr6_1:85579481-85579500 Msa0896080:intron 35.0%
! TATTGGTAGCAAAAATCCCT+TGG - chr6_1:85579566-85579585 Msa0896080:intron 35.0%
CTTGACACTGCAAATATAAC+AGG + chr6_1:85579655-85579674 None:intergenic 35.0%
TGTCAAGAATGCGTTAGATT+TGG - chr6_1:85579668-85579687 Msa0896080:intron 35.0%
! ATTGCTTGGTAATTGTTGGT+TGG - chr6_1:85579777-85579796 Msa0896080:CDS 35.0%
ACCAGCTATAGTGAATATCT+TGG - chr6_1:85579830-85579849 Msa0896080:intron 35.0%
CAGACAGAGAATACAATATG+TGG + chr6_1:85579914-85579933 None:intergenic 35.0%
GGTATAGAAGACAAGAGAAG+TGG + chr6_1:85579179-85579198 None:intergenic 40.0%
TAGTATGGGTTTGGACTTTG+AGG + chr6_1:85579200-85579219 None:intergenic 40.0%
ATACATCTTGTCCTCCCAAT+AGG - chr6_1:85579274-85579293 Msa0896080:three_prime_UTR 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:85579291-85579310 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:85579292-85579311 None:intergenic 40.0%
CATGAAATTCCAGCCTTCTT+TGG - chr6_1:85579480-85579499 Msa0896080:intron 40.0%
! TTTTATAGCCCAAAGAAGGC+TGG + chr6_1:85579492-85579511 None:intergenic 40.0%
! TAGTTTGAAGTCAACGCCAA+GGG + chr6_1:85579585-85579604 None:intergenic 40.0%
CAACAACGAAGATGAGTGAA+AGG - chr6_1:85579722-85579741 Msa0896080:CDS 40.0%
AATTGTTGCTCAGCATTGCT+TGG - chr6_1:85579763-85579782 Msa0896080:CDS 40.0%
! CAGCATTGCTTGGTAATTGT+TGG - chr6_1:85579773-85579792 Msa0896080:CDS 40.0%
GCCAAGATATTCACTATAGC+TGG + chr6_1:85579834-85579853 None:intergenic 40.0%
GCTATAGTGAATATCTTGGC+TGG - chr6_1:85579834-85579853 Msa0896080:intron 40.0%
GCATAAGGGATTGTAAGAAC+TGG - chr6_1:85579858-85579877 Msa0896080:intron 40.0%
GGTATAGAAGACAAGAGAAG+TGG + chr6_1:85579179-85579198 None:intergenic 40.0%
TAGTATGGGTTTGGACTTTG+AGG + chr6_1:85579200-85579219 None:intergenic 40.0%
ATACATCTTGTCCTCCCAAT+AGG - chr6_1:85579274-85579293 Msa0896080:three_prime_UTR 40.0%
GTTCTTCAGCTTCTCCTATT+GGG + chr6_1:85579291-85579310 None:intergenic 40.0%
TGTTCTTCAGCTTCTCCTAT+TGG + chr6_1:85579292-85579311 None:intergenic 40.0%
CATGAAATTCCAGCCTTCTT+TGG - chr6_1:85579480-85579499 Msa0896080:intron 40.0%
! TTTTATAGCCCAAAGAAGGC+TGG + chr6_1:85579492-85579511 None:intergenic 40.0%
! TAGTTTGAAGTCAACGCCAA+GGG + chr6_1:85579585-85579604 None:intergenic 40.0%
CAACAACGAAGATGAGTGAA+AGG - chr6_1:85579722-85579741 Msa0896080:CDS 40.0%
AATTGTTGCTCAGCATTGCT+TGG - chr6_1:85579763-85579782 Msa0896080:CDS 40.0%
! CAGCATTGCTTGGTAATTGT+TGG - chr6_1:85579773-85579792 Msa0896080:CDS 40.0%
GCCAAGATATTCACTATAGC+TGG + chr6_1:85579834-85579853 None:intergenic 40.0%
GCTATAGTGAATATCTTGGC+TGG - chr6_1:85579834-85579853 Msa0896080:intron 40.0%
GCATAAGGGATTGTAAGAAC+TGG - chr6_1:85579858-85579877 Msa0896080:intron 40.0%
!! AGACTGGCTTTGCAAGAAGT+CGG - chr6_1:85579339-85579358 Msa0896080:three_prime_UTR 45.0%
TGCAAGAAGTCGGTATCTTC+TGG - chr6_1:85579349-85579368 Msa0896080:exon 45.0%
! CTTCTGGAGACACTATGTAC+TGG - chr6_1:85579365-85579384 Msa0896080:CDS 45.0%
! TGGAGACACTATGTACTGGT+GGG - chr6_1:85579369-85579388 Msa0896080:CDS 45.0%
! CTAGTTTGAAGTCAACGCCA+AGG + chr6_1:85579586-85579605 None:intergenic 45.0%
AATATCTTGGCTGGCGCATA+AGG - chr6_1:85579843-85579862 Msa0896080:intron 45.0%
ATATCTTGGCTGGCGCATAA+GGG - chr6_1:85579844-85579863 Msa0896080:intron 45.0%
!! AGACTGGCTTTGCAAGAAGT+CGG - chr6_1:85579339-85579358 Msa0896080:three_prime_UTR 45.0%
TGCAAGAAGTCGGTATCTTC+TGG - chr6_1:85579349-85579368 Msa0896080:exon 45.0%
! CTTCTGGAGACACTATGTAC+TGG - chr6_1:85579365-85579384 Msa0896080:CDS 45.0%
! TGGAGACACTATGTACTGGT+GGG - chr6_1:85579369-85579388 Msa0896080:CDS 45.0%
! CTAGTTTGAAGTCAACGCCA+AGG + chr6_1:85579586-85579605 None:intergenic 45.0%
AATATCTTGGCTGGCGCATA+AGG - chr6_1:85579843-85579862 Msa0896080:intron 45.0%
ATATCTTGGCTGGCGCATAA+GGG - chr6_1:85579844-85579863 Msa0896080:intron 45.0%
AGAGAAGTGGAGTGAGAGAG+TGG + chr6_1:85579166-85579185 None:intergenic 50.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:85579288-85579307 None:intergenic 50.0%
TGGAAAAGACTGTGCCAGAC+TGG - chr6_1:85579323-85579342 Msa0896080:three_prime_UTR 50.0%
GACTTCTTGCAAAGCCAGTC+TGG + chr6_1:85579340-85579359 None:intergenic 50.0%
CTGGAGACACTATGTACTGG+TGG - chr6_1:85579368-85579387 Msa0896080:CDS 50.0%
AGAGAAGTGGAGTGAGAGAG+TGG + chr6_1:85579166-85579185 None:intergenic 50.0%
CTTCAGCTTCTCCTATTGGG+AGG + chr6_1:85579288-85579307 None:intergenic 50.0%
TGGAAAAGACTGTGCCAGAC+TGG - chr6_1:85579323-85579342 Msa0896080:three_prime_UTR 50.0%
GACTTCTTGCAAAGCCAGTC+TGG + chr6_1:85579340-85579359 None:intergenic 50.0%
CTGGAGACACTATGTACTGG+TGG - chr6_1:85579368-85579387 Msa0896080:CDS 50.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 85579159 85579943 85579159 ID=Msa0896080;Name=Msa0896080
chr6_1 mRNA 85579159 85579943 85579159 ID=Msa0896080-mRNA-1;Parent=Msa0896080;Name=Msa0896080-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.5|0.33|0.66|0.5|0.33|3|203|71
chr6_1 exon 85579159 85579434 85579159 ID=Msa0896080-mRNA-1:exon:14410;Parent=Msa0896080-mRNA-1
chr6_1 exon 85579716 85579830 85579716 ID=Msa0896080-mRNA-1:exon:14409;Parent=Msa0896080-mRNA-1
chr6_1 exon 85579916 85579943 85579916 ID=Msa0896080-mRNA-1:exon:14408;Parent=Msa0896080-mRNA-1
chr6_1 CDS 85579916 85579943 85579916 ID=Msa0896080-mRNA-1:cds;Parent=Msa0896080-mRNA-1
chr6_1 CDS 85579716 85579830 85579716 ID=Msa0896080-mRNA-1:cds;Parent=Msa0896080-mRNA-1
chr6_1 CDS 85579362 85579434 85579362 ID=Msa0896080-mRNA-1:cds;Parent=Msa0896080-mRNA-1
chr6_1 three_prime_UTR 85579159 85579361 85579159 ID=Msa0896080-mRNA-1:three_prime_utr;Parent=Msa0896080-mRNA-1
Gene Sequence

>Msa0896080

ATGTCCACTCTCTCACTCCACTTCTCTTTTATACATCTTGTCCTCCCAATAGGAGAAGCTGAAGAACAGATTGAGATTTTGGAAAAGACTGTGCCAGACTGGCTTTGCAAGAAGTCGGTATCTTCTGGAGACACTATGTACTGTGTCAAGAATGCGTTAGATTTGGACTCTGTTCGATCGACGCTTCTCAGCAATGTCAACAACGAAGATGAGTGA

Protein sequence

>Msa0896080

MSTLSLHFSFIHLVLPIGEAEEQIEILEKTVPDWLCKKSVSSGDTMYCVKNALDLDSVRSTLLSNVNNEDE*