Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187480 | ABE88115.1 | 79.024 | 205 | 35 | 1 | 195 | 399 | 8 | 204 | 2.62e-94 | 313 |
| Msa1187480 | ABE88115.1 | 91.026 | 390 | 21 | 2 | 505 | 880 | 14 | 403 | 0.0 | 717 |
| Msa1187480 | ABE88115.1 | 91.156 | 147 | 7 | 1 | 363 | 503 | 261 | 407 | 4.92e-78 | 270 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187480 | sp|Q8GYA4|CRK10_ARATH | 56.308 | 325 | 131 | 3 | 565 | 879 | 341 | 664 | 4.83e-120 | 382 |
| Msa1187480 | sp|Q8GYA4|CRK10_ARATH | 36.290 | 372 | 175 | 8 | 40 | 368 | 88 | 440 | 1.01e-58 | 216 |
| Msa1187480 | sp|Q8GYA4|CRK10_ARATH | 45.890 | 146 | 68 | 3 | 363 | 498 | 520 | 664 | 2.08e-28 | 125 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0060690 | Msa1187480 | 0.807991 | 3.856279e-50 | -8.615850e-47 |
| Msa0105440 | Msa1187480 | 0.823440 | 1.383316e-53 | -8.615850e-47 |
| Msa0121300 | Msa1187480 | 0.803933 | 2.752249e-49 | -8.615850e-47 |
| Msa0151660 | Msa1187480 | 0.809764 | 1.610533e-50 | -8.615850e-47 |
| Msa0166190 | Msa1187480 | 0.813597 | 2.361431e-51 | -8.615850e-47 |
| Msa0171330 | Msa1187480 | 0.812212 | 4.749721e-51 | -8.615850e-47 |
| Msa0224870 | Msa1187480 | 0.836032 | 1.186529e-56 | -8.615850e-47 |
| Msa0254470 | Msa1187480 | 0.806264 | 8.948682e-50 | -8.615850e-47 |
| Msa0293720 | Msa1187480 | 0.821748 | 3.424150e-53 | -8.615850e-47 |
| Msa0298000 | Msa1187480 | 0.811061 | 8.450922e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187480 | MtrunA17_Chr8g0354421 | 91.026 | 390 | 21 | 2 | 505 | 880 | 289 | 678 | 0.0 | 715 |
| Msa1187480 | MtrunA17_Chr8g0354421 | 74.839 | 465 | 43 | 4 | 1 | 399 | 23 | 479 | 0.0 | 680 |
| Msa1187480 | MtrunA17_Chr8g0354421 | 91.156 | 147 | 7 | 1 | 363 | 503 | 536 | 682 | 1.07e-78 | 269 |
| Msa1187480 | MtrunA17_Chr8g0354441 | 84.615 | 390 | 46 | 2 | 505 | 880 | 276 | 665 | 0.0 | 672 |
| Msa1187480 | MtrunA17_Chr8g0354441 | 61.064 | 470 | 103 | 6 | 1 | 399 | 6 | 466 | 0.0 | 543 |
| Msa1187480 | MtrunA17_Chr8g0354441 | 86.395 | 147 | 14 | 1 | 363 | 503 | 523 | 669 | 1.33e-75 | 260 |
| Msa1187480 | MtrunA17_Chr8g0354431 | 81.892 | 370 | 53 | 2 | 525 | 880 | 288 | 657 | 0.0 | 617 |
| Msa1187480 | MtrunA17_Chr8g0354431 | 65.501 | 429 | 81 | 3 | 1 | 362 | 1 | 429 | 1.58e-177 | 528 |
| Msa1187480 | MtrunA17_Chr8g0354431 | 87.755 | 147 | 12 | 1 | 363 | 503 | 515 | 661 | 2.90e-75 | 259 |
| Msa1187480 | MtrunA17_Chr8g0354281 | 74.510 | 408 | 91 | 4 | 479 | 880 | 249 | 649 | 0.0 | 604 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187480 | AT4G23180.1 | 56.308 | 325 | 131 | 3 | 565 | 879 | 341 | 664 | 4.91e-121 | 382 |
| Msa1187480 | AT4G23180.1 | 36.290 | 372 | 175 | 8 | 40 | 368 | 88 | 440 | 1.02e-59 | 216 |
| Msa1187480 | AT4G23180.1 | 45.890 | 146 | 68 | 3 | 363 | 498 | 520 | 664 | 2.12e-29 | 125 |
| Msa1187480 | AT4G05200.1 | 47.826 | 414 | 188 | 5 | 490 | 880 | 263 | 671 | 5.77e-118 | 374 |
| Msa1187480 | AT4G05200.1 | 35.101 | 396 | 195 | 7 | 17 | 362 | 50 | 433 | 7.92e-68 | 239 |
| Msa1187480 | AT4G05200.1 | 39.216 | 153 | 77 | 2 | 363 | 499 | 519 | 671 | 1.06e-24 | 110 |
| Msa1187480 | AT4G05200.2 | 47.826 | 414 | 188 | 5 | 490 | 880 | 281 | 689 | 9.42e-118 | 374 |
| Msa1187480 | AT4G05200.2 | 35.101 | 396 | 195 | 7 | 17 | 362 | 68 | 451 | 9.51e-68 | 239 |
| Msa1187480 | AT4G05200.2 | 39.216 | 153 | 77 | 2 | 363 | 499 | 537 | 689 | 1.03e-24 | 110 |
| Msa1187480 | AT4G21390.1 | 60.841 | 309 | 114 | 2 | 556 | 858 | 513 | 820 | 1.46e-117 | 378 |
Find 168 sgRNAs with CRISPR-Local
Find 701 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGACTCGGAAAAGTCAT+CGG | 0.587221 | 8_1:-27186777 | None:intergenic |
| AAATGACTCTGAAAAGTCAT+CGG | 0.600769 | 8_1:-27178833 | None:intergenic |
| AAATTAGGAGAAGGTGGATT+TGG | 0.316113 | 8_1:+27178856 | Msa1187480:CDS |
| AAATTAGGAGAAGGTGGATT+TGG | 0.316113 | 8_1:+27186800 | Msa1187480:CDS |
| AACCCAGCCATAGCGTATTC+TGG | 0.314405 | 8_1:-27187736 | None:intergenic |
| AACGTCAAGTCAAGGTTCAG+AGG | 0.602578 | 8_1:+27186980 | Msa1187480:CDS |
| AACTCCAAAGCTGAAAACAT+CGG | 0.629248 | 8_1:-27179825 | None:intergenic |
| AACTCCAAAGCTGAAAACAT+CGG | 0.629248 | 8_1:-27187773 | None:intergenic |
| AACTCGCCCGTTGCCCAAAC+GGG | 0.412035 | 8_1:-27177299 | None:intergenic |
| AACTGAAAATGCTGGTTGCG+TGG | 0.559495 | 8_1:-27180281 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAACTCAGATTCTGACTT+AGG | + | chr8_1:27176933-27176952 | Msa1187480:intron | 30.0% | |
| TGCTACAGTTACAAAACTTA+GGG | - | chr8_1:27177656-27177675 | None:intergenic | 30.0% | |
| AGTTCTATGCAATTCAAACT+AGG | - | chr8_1:27177887-27177906 | None:intergenic | 30.0% | |
| AAGCCTAAATATTGATGCAT+TGG | + | chr8_1:27178324-27178343 | Msa1187480:intron | 30.0% | |
| ACTTACTAAGATGATGATCA+AGG | - | chr8_1:27178475-27178494 | None:intergenic | 30.0% | |
| ACTATTACTGGAGAAAGAAT+AGG | + | chr8_1:27178532-27178551 | Msa1187480:CDS | 30.0% | |
| GAGTCATTTAAATTAGGAGA+AGG | + | chr8_1:27178847-27178866 | Msa1187480:CDS | 30.0% | |
| TCATTTAAATTAGGAGAAGG+TGG | + | chr8_1:27178850-27178869 | Msa1187480:CDS | 30.0% | |
| AAACGTACAAGTGAAATTCT+GGG | - | chr8_1:27178916-27178935 | None:intergenic | 30.0% | |
| ATGTACCCTGATAAAAATGA+AGG | - | chr8_1:27178973-27178992 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 27176765 | 27188329 | 27176765 | ID=Msa1187480;Name=Msa1187480 |
| chr8_1 | mRNA | 27176765 | 27188329 | 27176765 | ID=Msa1187480-mRNA-1;Parent=Msa1187480;Name=Msa1187480-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0.53|0.43|1|0.6|0.56|16|0|880 |
| chr8_1 | exon | 27176765 | 27176858 | 27176765 | ID=Msa1187480-mRNA-1:exon:9502;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27176982 | 27177220 | 27176982 | ID=Msa1187480-mRNA-1:exon:9503;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27177227 | 27177473 | 27177227 | ID=Msa1187480-mRNA-1:exon:9504;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27178442 | 27178564 | 27178442 | ID=Msa1187480-mRNA-1:exon:9505;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27178719 | 27178891 | 27178719 | ID=Msa1187480-mRNA-1:exon:9506;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27178986 | 27179196 | 27178986 | ID=Msa1187480-mRNA-1:exon:9507;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27179792 | 27179925 | 27179792 | ID=Msa1187480-mRNA-1:exon:9508;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27180088 | 27180187 | 27180088 | ID=Msa1187480-mRNA-1:exon:9509;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27180206 | 27180381 | 27180206 | ID=Msa1187480-mRNA-1:exon:9510;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27186433 | 27186550 | 27186433 | ID=Msa1187480-mRNA-1:exon:9511;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27186687 | 27186835 | 27186687 | ID=Msa1187480-mRNA-1:exon:9512;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27186933 | 27187143 | 27186933 | ID=Msa1187480-mRNA-1:exon:9513;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27187392 | 27187629 | 27187392 | ID=Msa1187480-mRNA-1:exon:9514;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27187723 | 27187873 | 27187723 | ID=Msa1187480-mRNA-1:exon:9515;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27188036 | 27188135 | 27188036 | ID=Msa1187480-mRNA-1:exon:9516;Parent=Msa1187480-mRNA-1 |
| chr8_1 | exon | 27188154 | 27188329 | 27188154 | ID=Msa1187480-mRNA-1:exon:9517;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27176765 | 27176858 | 27176765 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27176982 | 27177220 | 27176982 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27177227 | 27177473 | 27177227 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27178442 | 27178564 | 27178442 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27178719 | 27178891 | 27178719 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27178986 | 27179196 | 27178986 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27179792 | 27179925 | 27179792 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27180088 | 27180187 | 27180088 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27180206 | 27180381 | 27180206 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27186433 | 27186550 | 27186433 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27186687 | 27186835 | 27186687 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27186933 | 27187143 | 27186933 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27187392 | 27187629 | 27187392 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27187723 | 27187873 | 27187723 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27188036 | 27188135 | 27188036 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| chr8_1 | CDS | 27188154 | 27188329 | 27188154 | ID=Msa1187480-mRNA-1:cds;Parent=Msa1187480-mRNA-1 |
| Gene Sequence |
>Msa1187480 ATGACTTGTACGACAAGGTTGCTAAGCTTCATTCTCCTTCATTACTTCCTCTTCATGATCCTAACAACAGCACAATCACCATTTTACTTGTACAGTAGCAACAACAGCAATGATCATGACAATGTTTATGGGTTATACGGTTGTCGGGGTGACATCACAGGAAGTTTCTGCCGATTCTGTATCAACACTGCTGTTAGAGAAATCGCTCAACGTTGTCCTAACAGTGTTTCGGCTTTGATATGGTATGATGTATGCGTCATGGGTTACACTAACCAGAATACCTCAGGAAAAGTTATTGTAACACCGTCATGGAATGTAACTGGATCCAGAAATGACTCAACAGAACTTGGGAAAGCTGAGAATAACATGATGAGTTTGATACGCAAAGTGACTACAGAATCCAGTCCCGTTTGGGCAACGGGCGAGTTCATTTGGAGTGACACCGAGAAAAGATATGGTTTGGTGCAGTGTAATAGAGACCTTAGTAAGGATGGGTGCAAACAGTGTCTAGAGGCTATGTTGGATTTAGTTCCTCGGTGTTGTGGAACGAAGGTAGCGTGGGCAGTTATGTCTCCTAGCTGCAAGCAGGAAGGTGCAAGTAAAACTAAAACCTTGATCATCATCTTAGTAAGTATAACGGTGGCAGTAGCTCTACTGAGCTGTTGTGTCTACTATTACTGGAGAAAGAATAGGTTAAGTAAAGGTGGTATGCTGTTGAGGACCATTACTCGTATATCTTTCCGTAATCAGGTTCAAAGACAGGATTCATTCAATGGAGAACTTCCTACAATCTCTTTAAATGTAATTCAACAAAGTACCGATGACTTTTCAGAGTCATTTAAATTAGGAGAAGGTGGATTTGGCCCTGTTTACAAGGGTACATTACCAGATGGCACAGAAGTTGCTGTCAAAAGGCTTTCGGAAATGTCGAGTCAAGGTTCAGAGGAGTTCAAGAATGAAGTAATATTCATAGCTAAACTGCAACATAGAAACCTTGTGAAACTCTTGGGTTACTGCATTGAGGGAGATGAAAAAATACTAGTATATGAGTACATGCCGAATTCAAGCCTTGACTTTCACCTTTTCAAATACGCTATGGCTGGGTTATTTTCAGTGAAATCCGATGTTTTCAGCTTTGGAGTTCTTGTGTTAGAAATAATTTATGGAAAAAGAAATGGCGACTTCTTTCTTTCAGAACATATGCAAAGTCTTCTATTATATACTTGGAAACTTTGGTGTGAGGGGAAAAGTTTGGAATTAATCGATCCATTTCACCAAAAGACATACATTGAAAGTGAAGTCTTGAAGTGTATACACATTGAATATGCAGCAGATCGACCAACGATGTCGACCGTTGTTTGCATGCTAGGGAGTGACACAGTAGACCTTCCGAAACCCACGCAACCAGCATTTTCAGTTGGCAGAAAGTCCAAGAATGAAGATCAAATTTCAAAAAATTCTAAGGATAATTCTGTTGATGAAGAAACCATAACTATTGCAAGCAGGAAGGTACAAGCTAAAACCTTGATCATCATCTTCGTAGGTATAACAGTGGCAGTAGCTCTACTAAGCTGTTGTGTCTACTATAACCGGAGAAAGAATAGGTTAAGTAAAGGTGGTATGCTGTTGAGGACCATTATTCAAAGACAGGATTCATTCAATGGAGAACTTCCTACAATCCCTTTAACTGTAATTCAACAAAGTACCGATGACTTTTCCGAGTCATTTAAATTAGGAGAAGGTGGATTTGGCCCAGTTTACAAGGGTACTTTACCAGATGGCACAAAAGTTGCTGTCAAAAGGCTTTCGGAAACGTCAAGTCAAGGTTCAGAGGAGTTCAAGAATGAAGTAATATTCATAGCTAAACTGCAACATAGAAACCTTACGAAACTCTTGAATTACTGCATTGAGGGAGATGAAAAAATACTAGTATATGAGTACATGCCGAATTCAAGCCTTGACTTTCATCTTTTCAATAAGGAAAAACATAAGCATCTAGATTGGAAATTACGACTAAGCATTATCAATGGAATTGCAAGAGGACTGCTATCCCTTCATGAGGATTCTCGACTTAGAGTAATCCACAGAGATCTGAAAGCGAGCAATGTTCTTCTGGATGATGAAATGAATCCAAAAATATCAGATTTTGGATTGGCAAGAACATTTGATAAAGACCAATGTCAGACAAAAACAAAAAGAGTTTTTGGCACCTATGGATACATGGCTCCAGAATACGCTATGGCTGGGTTATTTTCAGTGAAATCCGATGTTTTCAGCTTTGGAGTTCTTGTGTTAGAAATAATTTATGGAAAAAGAAATGGCGACTTCTTTCTTTCAGAACATATGCAAAGTCTTCTATTATATACTTGGAAACTTTGGTGTGAGGGGAAAAGTTTGGAATTAATCGATCCATTTCACCAAAAGACATACATTGAAAGTGAAGTCTTGAAGTGTATACACATTGAATATGCAGCAGATCGACCAACGATGTCGACCGTTGTTTGCATGCTAGGAAGTGACACAATAGACCTTCCGAAACCCACGCAACCAGCATTTTCAGTTGGCAGAAAGTCCAAGAATGAAGATCAAATTTCAAAAAATTCTAAGGATAATTCTGTTGATGAAGAAACCATAACTATT |
| Protein sequence |
>Msa1187480 MTCTTRLLSFILLHYFLFMILTTAQSPFYLYSSNNSNDHDNVYGLYGCRGDITGSFCRFCINTAVREIAQRCPNSVSALIWYDVCVMGYTNQNTSGKVIVTPSWNVTGSRNDSTELGKAENNMMSLIRKVTTESSPVWATGEFIWSDTEKRYGLVQCNRDLSKDGCKQCLEAMLDLVPRCCGTKVAWAVMSPSCKQEGASKTKTLIIILVSITVAVALLSCCVYYYWRKNRLSKGGMLLRTITRISFRNQVQRQDSFNGELPTISLNVIQQSTDDFSESFKLGEGGFGPVYKGTLPDGTEVAVKRLSEMSSQGSEEFKNEVIFIAKLQHRNLVKLLGYCIEGDEKILVYEYMPNSSLDFHLFKYAMAGLFSVKSDVFSFGVLVLEIIYGKRNGDFFLSEHMQSLLLYTWKLWCEGKSLELIDPFHQKTYIESEVLKCIHIEYAADRPTMSTVVCMLGSDTVDLPKPTQPAFSVGRKSKNEDQISKNSKDNSVDEETITIASRKVQAKTLIIIFVGITVAVALLSCCVYYNRRKNRLSKGGMLLRTIIQRQDSFNGELPTIPLTVIQQSTDDFSESFKLGEGGFGPVYKGTLPDGTKVAVKRLSETSSQGSEEFKNEVIFIAKLQHRNLTKLLNYCIEGDEKILVYEYMPNSSLDFHLFNKEKHKHLDWKLRLSIINGIARGLLSLHEDSRLRVIHRDLKASNVLLDDEMNPKISDFGLARTFDKDQCQTKTKRVFGTYGYMAPEYAMAGLFSVKSDVFSFGVLVLEIIYGKRNGDFFLSEHMQSLLLYTWKLWCEGKSLELIDPFHQKTYIESEVLKCIHIEYAADRPTMSTVVCMLGSDTIDLPKPTQPAFSVGRKSKNEDQISKNSKDNSVDEETITI |
