AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0105440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0105440 MtrunA17_Chr1g0160381 93.464 153 10 0 1 153 5 157 1.75e-102 290
Msa0105440 MtrunA17_Chr1g0160371 86.928 153 20 0 1 153 1 153 4.61e-97 276
Msa0105440 MtrunA17_Chr1g0160391 58.065 155 60 2 1 150 1 155 8.71e-61 185
Msa0105440 MtrunA17_Chr1g0160351 78.261 92 20 0 62 153 3 94 3.90e-48 150
Msa0105440 MtrunA17_Chr8g0355721 40.523 153 88 2 1 152 1 151 4.50e-38 127
Msa0105440 MtrunA17_Chr8g0355781 39.869 153 89 2 1 152 1 151 6.19e-38 126
Msa0105440 MtrunA17_Chr8g0355661 39.216 153 89 3 1 152 1 150 1.96e-36 123
Msa0105440 MtrunA17_Chr8g0355811 38.562 153 90 3 1 152 1 150 3.24e-36 122
Msa0105440 MtrunA17_Chr8g0355821 38.562 153 90 3 1 152 1 150 4.67e-36 122
Msa0105440 MtrunA17_Chr8g0355771 38.562 153 90 3 1 152 1 150 5.40e-36 122
Msa0105440 MtrunA17_Chr8g0355761 36.601 153 94 2 1 152 1 151 9.76e-36 121
Msa0105440 MtrunA17_Chr8g0355741 37.662 154 93 2 1 153 1 152 4.06e-35 119
Msa0105440 MtrunA17_Chr8g0355801 37.255 153 92 3 1 152 1 150 2.62e-34 117
Msa0105440 MtrunA17_Chr8g0355791 37.255 153 92 3 1 152 1 150 3.95e-34 117
Msa0105440 MtrunA17_Chr8g0355641 38.961 154 91 2 1 153 1 152 4.58e-34 117
Msa0105440 MtrunA17_Chr1g0160801 37.662 154 86 5 1 152 1 146 3.31e-28 101
Msa0105440 MtrunA17_Chr8g0339261 35.897 156 94 4 1 153 1 153 4.04e-28 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0105440 AT1G70890.1 37.662 154 92 3 2 153 6 157 5.64e-32 112
Msa0105440 AT5G28010.1 38.961 154 90 3 2 153 14 165 2.42e-31 110
Msa0105440 AT5G28010.2 38.961 154 90 3 2 153 14 165 2.42e-31 110
Msa0105440 AT1G70840.1 35.065 154 96 3 2 153 19 170 1.20e-30 108
Msa0105440 AT1G70830.5 37.013 154 93 3 2 153 21 172 3.93e-30 107
Msa0105440 AT1G70830.3 37.013 154 93 3 2 153 21 172 9.82e-30 107
Msa0105440 AT1G70830.2 37.013 154 93 3 2 153 21 172 4.24e-29 107
Msa0105440 AT1G70830.1 37.013 154 93 3 2 153 21 172 1.83e-28 107
Msa0105440 AT1G70830.1 36.364 154 94 3 2 153 183 334 4.03e-27 103
Msa0105440 AT1G70830.4 36.364 154 94 3 2 153 136 287 9.91e-28 104
Msa0105440 AT1G70830.4 36.458 96 58 2 59 153 32 125 9.64e-14 67.0
Msa0105440 AT1G70850.2 34.211 152 96 3 2 151 8 157 1.42e-26 100
Msa0105440 AT1G70850.3 34.211 152 96 3 2 151 8 157 5.68e-26 100
Msa0105440 AT1G70850.3 35.664 143 88 3 13 153 175 315 4.83e-25 98.2
Msa0105440 AT1G70850.1 34.211 152 96 3 2 151 8 157 5.68e-26 100
Msa0105440 AT1G70850.1 35.664 143 88 3 13 153 175 315 4.83e-25 98.2
Msa0105440 AT5G28000.1 33.766 154 98 3 2 153 12 163 6.50e-26 96.7
Msa0105440 AT1G70880.1 32.468 154 100 3 2 153 7 158 7.70e-25 94.0
Msa0105440 AT1G23130.1 27.451 153 108 2 2 153 9 159 1.70e-24 93.2
Msa0105440 AT1G14930.1 35.294 153 95 3 1 152 1 150 1.13e-23 90.9
Msa0105440 AT1G70870.1 36.842 152 82 5 1 152 1 138 2.74e-23 89.4
Msa0105440 AT1G35260.1 31.293 147 96 3 7 151 6 149 2.86e-23 89.7
Msa0105440 AT1G23120.1 31.373 153 99 3 1 153 1 147 9.23e-21 83.2
Msa0105440 AT1G23120.2 31.373 153 99 3 1 153 51 197 3.13e-20 83.2
Msa0105440 AT1G14950.1 30.065 153 103 3 1 152 1 150 6.02e-19 78.6
Msa0105440 AT4G14060.1 30.065 153 103 3 1 152 1 150 1.39e-18 77.4
Msa0105440 AT1G35310.1 30.282 142 95 3 13 153 12 150 7.70e-18 75.5
Msa0105440 AT1G24020.1 35.802 162 85 7 1 153 1 152 3.45e-17 73.9
Msa0105440 AT1G24020.2 35.802 162 85 7 1 153 1 152 3.45e-17 73.9
Msa0105440 AT2G01520.1 29.412 153 104 3 1 152 1 150 4.98e-17 73.6
Msa0105440 AT1G14940.1 29.605 152 103 3 1 151 1 149 5.22e-17 73.6
Msa0105440 AT1G30990.1 27.632 152 105 3 1 151 1 148 3.50e-16 71.2
Msa0105440 AT2G01530.1 29.412 153 104 3 1 152 1 150 2.13e-15 69.3
Msa0105440 AT1G14960.1 28.105 153 106 3 1 152 1 150 2.91e-15 68.9
Msa0105440 AT3G26460.1 28.105 153 107 2 1 152 1 151 6.47e-13 62.8
Msa0105440 AT3G26450.1 28.571 154 105 4 1 152 1 151 9.35e-13 62.4
Msa0105440 AT4G23670.1 29.412 153 104 3 1 152 1 150 1.69e-12 61.6
Msa0105440 AT4G23680.1 27.451 153 107 3 1 152 1 150 7.20e-12 60.1

Find 30 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTACTTCTGTGGTGATATT+AGG 0.304651 1_3:-13959524 None:intergenic
TGAGAGTTACAAGAGTTTAA+AGG 0.313337 1_3:+13960190 Msa0105440:CDS
ATGGTGGTGGGATTGTTAAA+TGG 0.324878 1_3:+13960240 Msa0105440:CDS
GAAGGTGACTGGGAAAATAT+TGG 0.418929 1_3:+13959563 Msa0105440:CDS
ATACTTGGACCTTGCTGTAA+AGG 0.426075 1_3:+13960316 Msa0105440:CDS
GCAAGGTCCAAGTATGAATC+AGG 0.441032 1_3:-13960308 None:intergenic
TGGTGGTGGGATTGTTAAAT+GGG 0.470252 1_3:+13960241 Msa0105440:CDS
TGTATCTCCTGATTCATACT+TGG 0.511390 1_3:+13960301 Msa0105440:CDS
CATGGCTTTAAGTGGGAAAG+TGG 0.512659 1_3:+13959430 None:intergenic
CGAATATGAGAAGCTAAAAG+AGG 0.515741 1_3:+13960268 Msa0105440:CDS
GTAGAGTGCATGAAGGTGAC+TGG 0.523294 1_3:+13959552 Msa0105440:CDS
AGAAGAGACATGGCTTTAAG+TGG 0.526395 1_3:+13959422 None:intergenic
ATTCATAAAGAACATGGTGG+TGG 0.537340 1_3:+13960227 Msa0105440:CDS
ATATTGGTTCTATCAAGCAC+TGG 0.538736 1_3:+13959579 Msa0105440:CDS
TTCATAAAGAACATGGTGGT+GGG 0.570285 1_3:+13960228 Msa0105440:CDS
AATATCACCACAGAAGTACA+TGG 0.570466 1_3:+13959527 Msa0105440:CDS
AAGCTAAAAGAGGAAACTAC+AGG 0.574676 1_3:+13960278 Msa0105440:CDS
TATTGGTTCTATCAAGCACT+GGG 0.594586 1_3:+13959580 Msa0105440:CDS
TCTACCTCCATGTACTTCTG+TGG 0.594643 1_3:-13959534 None:intergenic
TAGAGTGCATGAAGGTGACT+GGG 0.605256 1_3:+13959553 Msa0105440:CDS
CAAGTGATTCATAAAGAACA+TGG 0.605539 1_3:+13960221 Msa0105440:CDS
TATTGATTCTCATCTTGTCA+AGG 0.605627 1_3:+13960349 Msa0105440:CDS
GGCTTTAAGTGGGAAAGTGG+AGG 0.606303 1_3:+13959433 Msa0105440:CDS
AAGCACTGGGAATTTACAGT+AGG 0.622307 1_3:+13959593 Msa0105440:CDS
GAAGAGACATGGCTTTAAGT+GGG 0.623024 1_3:+13959423 None:intergenic
CATGGAGGTAGAGTGCATGA+AGG 0.659264 1_3:+13959545 Msa0105440:CDS
GAAGTAGAGATTCAAGCACC+TGG 0.664321 1_3:+13959458 Msa0105440:CDS
ATATTATAGAACTTAGCACC+AGG 0.680852 1_3:-13959476 None:intergenic
GTGATTCATAAAGAACATGG+TGG 0.735641 1_3:+13960224 Msa0105440:CDS
ATCACCACAGAAGTACATGG+AGG 0.776492 1_3:+13959530 Msa0105440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATATGTTTTGTTTACAT+TGG + chr1_3:13960007-13960026 Msa0105440:intron 15.0%
!!! TAAATATGTTTTGTTTACAT+TGG + chr1_3:13960007-13960026 Msa0105440:intron 15.0%
!! TAGTAAAACTATGAAACATA+AGG - chr1_3:13959629-13959648 None:intergenic 20.0%
!! AGTGTTTATAAATGACAATT+TGG - chr1_3:13959818-13959837 None:intergenic 20.0%
!! TCATTTGCTTAAAAAAAACA+AGG + chr1_3:13959964-13959983 Msa0105440:intron 20.0%
!!! TTATTTATTTTCCCTAATCA+TGG + chr1_3:13960044-13960063 Msa0105440:intron 20.0%
!!! ATCATATATAGCATTTTTGA+TGG + chr1_3:13960158-13960177 Msa0105440:CDS 20.0%
!! TAGTAAAACTATGAAACATA+AGG - chr1_3:13959629-13959648 None:intergenic 20.0%
!! AGTGTTTATAAATGACAATT+TGG - chr1_3:13959818-13959837 None:intergenic 20.0%
!! TCATTTGCTTAAAAAAAACA+AGG + chr1_3:13959964-13959983 Msa0105440:intron 20.0%
!!! TTATTTATTTTCCCTAATCA+TGG + chr1_3:13960044-13960063 Msa0105440:intron 20.0%
!!! ATCATATATAGCATTTTTGA+TGG + chr1_3:13960158-13960177 Msa0105440:CDS 20.0%
! GAATTTACAGTAGGTAATTA+AGG + chr1_3:13959602-13959621 Msa0105440:intron 25.0%
!! ACTATTTTATTGCTCATGAA+AGG + chr1_3:13959645-13959664 Msa0105440:intron 25.0%
! TAAATCAAGTTCCATGATTA+GGG - chr1_3:13960058-13960077 None:intergenic 25.0%
! TTAAATCAAGTTCCATGATT+AGG - chr1_3:13960059-13960078 None:intergenic 25.0%
! GAATTTACAGTAGGTAATTA+AGG + chr1_3:13959602-13959621 Msa0105440:intron 25.0%
!! ACTATTTTATTGCTCATGAA+AGG + chr1_3:13959645-13959664 Msa0105440:intron 25.0%
! TAAATCAAGTTCCATGATTA+GGG - chr1_3:13960058-13960077 None:intergenic 25.0%
! TTAAATCAAGTTCCATGATT+AGG - chr1_3:13960059-13960078 None:intergenic 25.0%
ATATTATAGAACTTAGCACC+AGG - chr1_3:13959479-13959498 None:intergenic 30.0%
! TAACATCAACAAAAGAGTGT+TGG - chr1_3:13959732-13959751 None:intergenic 30.0%
!! TTTGTTTTCTTCCTTCTGTA+GGG - chr1_3:13960094-13960113 None:intergenic 30.0%
TGAGAGTTACAAGAGTTTAA+AGG + chr1_3:13960190-13960209 Msa0105440:CDS 30.0%
CAAGTGATTCATAAAGAACA+TGG + chr1_3:13960221-13960240 Msa0105440:CDS 30.0%
!! TATTGATTCTCATCTTGTCA+AGG + chr1_3:13960349-13960368 Msa0105440:CDS 30.0%
ATATTATAGAACTTAGCACC+AGG - chr1_3:13959479-13959498 None:intergenic 30.0%
! TAACATCAACAAAAGAGTGT+TGG - chr1_3:13959732-13959751 None:intergenic 30.0%
!! TTTGTTTTCTTCCTTCTGTA+GGG - chr1_3:13960094-13960113 None:intergenic 30.0%
TGAGAGTTACAAGAGTTTAA+AGG + chr1_3:13960190-13960209 Msa0105440:CDS 30.0%
CAAGTGATTCATAAAGAACA+TGG + chr1_3:13960221-13960240 Msa0105440:CDS 30.0%
!! TATTGATTCTCATCTTGTCA+AGG + chr1_3:13960349-13960368 Msa0105440:CDS 30.0%
TGTACTTCTGTGGTGATATT+AGG - chr1_3:13959527-13959546 None:intergenic 35.0%
! AATATCACCACAGAAGTACA+TGG + chr1_3:13959527-13959546 Msa0105440:CDS 35.0%
!! ATATTGGTTCTATCAAGCAC+TGG + chr1_3:13959579-13959598 Msa0105440:CDS 35.0%
!! TATTGGTTCTATCAAGCACT+GGG + chr1_3:13959580-13959599 Msa0105440:CDS 35.0%
!! ATCAACAAAAGAGTGTTGGA+AGG - chr1_3:13959728-13959747 None:intergenic 35.0%
!!! CTTTGTTTTCTTCCTTCTGT+AGG - chr1_3:13960095-13960114 None:intergenic 35.0%
! TAGCATTTTTGATGGAGAAG+TGG + chr1_3:13960166-13960185 Msa0105440:CDS 35.0%
! AGCATTTTTGATGGAGAAGT+GGG + chr1_3:13960167-13960186 Msa0105440:CDS 35.0%
GTGATTCATAAAGAACATGG+TGG + chr1_3:13960224-13960243 Msa0105440:CDS 35.0%
ATTCATAAAGAACATGGTGG+TGG + chr1_3:13960227-13960246 Msa0105440:CDS 35.0%
TTCATAAAGAACATGGTGGT+GGG + chr1_3:13960228-13960247 Msa0105440:CDS 35.0%
CGAATATGAGAAGCTAAAAG+AGG + chr1_3:13960268-13960287 Msa0105440:CDS 35.0%
AAGCTAAAAGAGGAAACTAC+AGG + chr1_3:13960278-13960297 Msa0105440:CDS 35.0%
TGTATCTCCTGATTCATACT+TGG + chr1_3:13960301-13960320 Msa0105440:CDS 35.0%
TGTACTTCTGTGGTGATATT+AGG - chr1_3:13959527-13959546 None:intergenic 35.0%
! AATATCACCACAGAAGTACA+TGG + chr1_3:13959527-13959546 Msa0105440:CDS 35.0%
!! ATATTGGTTCTATCAAGCAC+TGG + chr1_3:13959579-13959598 Msa0105440:CDS 35.0%
!! TATTGGTTCTATCAAGCACT+GGG + chr1_3:13959580-13959599 Msa0105440:CDS 35.0%
!! ATCAACAAAAGAGTGTTGGA+AGG - chr1_3:13959728-13959747 None:intergenic 35.0%
!!! CTTTGTTTTCTTCCTTCTGT+AGG - chr1_3:13960095-13960114 None:intergenic 35.0%
! TAGCATTTTTGATGGAGAAG+TGG + chr1_3:13960166-13960185 Msa0105440:CDS 35.0%
! AGCATTTTTGATGGAGAAGT+GGG + chr1_3:13960167-13960186 Msa0105440:CDS 35.0%
GTGATTCATAAAGAACATGG+TGG + chr1_3:13960224-13960243 Msa0105440:CDS 35.0%
ATTCATAAAGAACATGGTGG+TGG + chr1_3:13960227-13960246 Msa0105440:CDS 35.0%
TTCATAAAGAACATGGTGGT+GGG + chr1_3:13960228-13960247 Msa0105440:CDS 35.0%
CGAATATGAGAAGCTAAAAG+AGG + chr1_3:13960268-13960287 Msa0105440:CDS 35.0%
AAGCTAAAAGAGGAAACTAC+AGG + chr1_3:13960278-13960297 Msa0105440:CDS 35.0%
TGTATCTCCTGATTCATACT+TGG + chr1_3:13960301-13960320 Msa0105440:CDS 35.0%
GAAGGTGACTGGGAAAATAT+TGG + chr1_3:13959563-13959582 Msa0105440:CDS 40.0%
!! AAGCACTGGGAATTTACAGT+AGG + chr1_3:13959593-13959612 Msa0105440:CDS 40.0%
!! ACAAAAGAGTGTTGGAAGGA+GGG - chr1_3:13959724-13959743 None:intergenic 40.0%
!! AACAAAAGAGTGTTGGAAGG+AGG - chr1_3:13959725-13959744 None:intergenic 40.0%
TACTTGTGAAACCCTACAGA+AGG + chr1_3:13960080-13960099 Msa0105440:intron 40.0%
!! ATGGTGGTGGGATTGTTAAA+TGG + chr1_3:13960240-13960259 Msa0105440:CDS 40.0%
!! TGGTGGTGGGATTGTTAAAT+GGG + chr1_3:13960241-13960260 Msa0105440:CDS 40.0%
! ATACTTGGACCTTGCTGTAA+AGG + chr1_3:13960316-13960335 Msa0105440:CDS 40.0%
! CTTTTGTGACCTTTACAGCA+AGG - chr1_3:13960328-13960347 None:intergenic 40.0%
GAAGGTGACTGGGAAAATAT+TGG + chr1_3:13959563-13959582 Msa0105440:CDS 40.0%
!! AAGCACTGGGAATTTACAGT+AGG + chr1_3:13959593-13959612 Msa0105440:CDS 40.0%
!! ACAAAAGAGTGTTGGAAGGA+GGG - chr1_3:13959724-13959743 None:intergenic 40.0%
!! AACAAAAGAGTGTTGGAAGG+AGG - chr1_3:13959725-13959744 None:intergenic 40.0%
TACTTGTGAAACCCTACAGA+AGG + chr1_3:13960080-13960099 Msa0105440:intron 40.0%
!! ATGGTGGTGGGATTGTTAAA+TGG + chr1_3:13960240-13960259 Msa0105440:CDS 40.0%
!! TGGTGGTGGGATTGTTAAAT+GGG + chr1_3:13960241-13960260 Msa0105440:CDS 40.0%
! ATACTTGGACCTTGCTGTAA+AGG + chr1_3:13960316-13960335 Msa0105440:CDS 40.0%
! CTTTTGTGACCTTTACAGCA+AGG - chr1_3:13960328-13960347 None:intergenic 40.0%
GAAGTAGAGATTCAAGCACC+TGG + chr1_3:13959458-13959477 Msa0105440:CDS 45.0%
! ATCACCACAGAAGTACATGG+AGG + chr1_3:13959530-13959549 Msa0105440:CDS 45.0%
TCTACCTCCATGTACTTCTG+TGG - chr1_3:13959537-13959556 None:intergenic 45.0%
TAGAGTGCATGAAGGTGACT+GGG + chr1_3:13959553-13959572 Msa0105440:CDS 45.0%
GCAAGGTCCAAGTATGAATC+AGG - chr1_3:13960311-13960330 None:intergenic 45.0%
GAAGTAGAGATTCAAGCACC+TGG + chr1_3:13959458-13959477 Msa0105440:CDS 45.0%
! ATCACCACAGAAGTACATGG+AGG + chr1_3:13959530-13959549 Msa0105440:CDS 45.0%
TCTACCTCCATGTACTTCTG+TGG - chr1_3:13959537-13959556 None:intergenic 45.0%
TAGAGTGCATGAAGGTGACT+GGG + chr1_3:13959553-13959572 Msa0105440:CDS 45.0%
GCAAGGTCCAAGTATGAATC+AGG - chr1_3:13960311-13960330 None:intergenic 45.0%
!! GGCTTTAAGTGGGAAAGTGG+AGG + chr1_3:13959433-13959452 Msa0105440:CDS 50.0%
CATGGAGGTAGAGTGCATGA+AGG + chr1_3:13959545-13959564 Msa0105440:CDS 50.0%
GTAGAGTGCATGAAGGTGAC+TGG + chr1_3:13959552-13959571 Msa0105440:CDS 50.0%
!! GGCTTTAAGTGGGAAAGTGG+AGG + chr1_3:13959433-13959452 Msa0105440:CDS 50.0%
CATGGAGGTAGAGTGCATGA+AGG + chr1_3:13959545-13959564 Msa0105440:CDS 50.0%
GTAGAGTGCATGAAGGTGAC+TGG + chr1_3:13959552-13959571 Msa0105440:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 13959431 13960376 13959431 ID=Msa0105440;Name=Msa0105440
chr1_3 mRNA 13959431 13960376 13959431 ID=Msa0105440-mRNA-1;Parent=Msa0105440;Name=Msa0105440-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0|0|1|1|1|2|0|153
chr1_3 exon 13959431 13959614 13959431 ID=Msa0105440-mRNA-1:exon:5464;Parent=Msa0105440-mRNA-1
chr1_3 exon 13960099 13960376 13960099 ID=Msa0105440-mRNA-1:exon:5465;Parent=Msa0105440-mRNA-1
chr1_3 CDS 13959431 13959614 13959431 ID=Msa0105440-mRNA-1:cds;Parent=Msa0105440-mRNA-1
chr1_3 CDS 13960099 13960376 13960099 ID=Msa0105440-mRNA-1:cds;Parent=Msa0105440-mRNA-1
Gene Sequence

>Msa0105440

ATGGCTTTAAGTGGGAAAGTGGAGGCTGAAGTAGAGATTCAAGCACCTGGTGCTAAGTTCTATAATATTTTCAGAAAGCAACTAGAACATCTTCCTAATATCACCACAGAAGTACATGGAGGTAGAGTGCATGAAGGTGACTGGGAAAATATTGGTTCTATCAAGCACTGGGAATTTACAGTAGAAGGAAGAAAACAAAGTGCTAAAGCAAAAATTGAGACTATAGATGATGACAATAAGATAATCATATATAGCATTTTTGATGGAGAAGTGGGTGAGAGTTACAAGAGTTTAAAGGCAATATTACAAGTGATTCATAAAGAACATGGTGGTGGGATTGTTAAATGGGCATACGAATATGAGAAGCTAAAAGAGGAAACTACAGGTGTATCTCCTGATTCATACTTGGACCTTGCTGTAAAGGTCACAAAAGATATTGATTCTCATCTTGTCAAGGAATAG

Protein sequence

>Msa0105440

MALSGKVEAEVEIQAPGAKFYNIFRKQLEHLPNITTEVHGGRVHEGDWENIGSIKHWEFTVEGRKQSAKAKIETIDDDNKIIIYSIFDGEVGESYKSLKAILQVIHKEHGGGIVKWAYEYEKLKEETTGVSPDSYLDLAVKVTKDIDSHLVKE*