AlfalfaGEDB | Msa1254240

M. sativa cultivar zhongmu-4 / Msa1254240

KEGG: K21777
GO: -
PFAM: PF00134.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254240	XP_003629162.1	98.618	434	5	1	1	433	1	434	0.0	894

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254240	sp\|P46278\|CCNB2_MEDSV	98.618	434	5	1	1	433	1	434	0.0	893

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0054690	Msa1254240	0.807163	5.781122e-50	-8.615850e-47
Msa0065020	Msa1254240	0.807867	4.098687e-50	-8.615850e-47
Msa0077340	Msa1254240	0.829312	5.524600e-55	-8.615850e-47
Msa0077560	Msa1254240	0.822710	2.047389e-53	-8.615850e-47
Msa0094780	Msa1254240	0.804082	2.562038e-49	-8.615850e-47
Msa0125360	Msa1254240	0.831616	1.508920e-55	-8.615850e-47
Msa0181650	Msa1254240	0.804764	1.846984e-49	-8.615850e-47
Msa0190730	Msa1254240	0.821875	3.200390e-53	-8.615850e-47
Msa0269210	Msa1254240	0.822130	2.792643e-53	-8.615850e-47
Msa0271880	Msa1254240	0.806205	9.208444e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254240	MtrunA17_Chr8g0370671	98.618	434	5	1	1	433	1	434	0.0	894
Msa1254240	MtrunA17_Chr5g0406741	73.086	431	103	5	6	431	6	428	0.0	638
Msa1254240	MtrunA17_Chr7g0255771	47.101	276	139	2	158	427	182	456	1.57e-84	266
Msa1254240	MtrunA17_Chr5g0441371	43.223	273	152	3	153	423	143	414	4.14e-75	240
Msa1254240	MtrunA17_Chr8g0357001	47.032	219	116	0	165	383	476	694	1.79e-59	206
Msa1254240	MtrunA17_Chr1g0148681	39.209	278	155	6	155	422	137	410	1.44e-57	194
Msa1254240	MtrunA17_Chr3g0138431	39.608	255	147	3	175	422	213	467	3.37e-55	190
Msa1254240	MtrunA17_Chr3g0125051	35.789	285	171	6	148	423	216	497	2.06e-53	186
Msa1254240	MtrunA17_Chr3g0125041	38.430	242	132	6	196	423	264	502	3.46e-51	180
Msa1254240	MtrunA17_Chr1g0152871	35.827	254	153	4	175	418	91	344	6.95e-49	170

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254240	AT1G20610.1	56.177	429	179	6	5	427	4	429	4.83e-167	476
Msa1254240	AT1G76310.1	56.552	435	169	9	5	427	4	430	1.84e-164	470
Msa1254240	AT1G76310.3	53.132	463	169	10	5	427	4	458	8.37e-158	454
Msa1254240	AT2G17620.1	59.259	405	149	7	29	427	29	423	2.39e-156	449
Msa1254240	AT4G35620.1	57.014	442	162	10	1	429	1	427	2.77e-150	434
Msa1254240	AT1G76310.2	55.357	392	155	9	5	384	4	387	3.09e-140	406
Msa1254240	AT4G35620.2	56.818	396	144	9	1	384	1	381	1.51e-131	384
Msa1254240	AT1G76310.4	54.955	333	130	9	5	325	4	328	2.27e-110	328
Msa1254240	AT3G11520.1	40.054	367	203	4	78	429	48	412	5.21e-85	266
Msa1254240	AT1G20590.1	68.023	172	55	0	233	404	63	234	7.40e-84	258

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Find 114 sgRNAs with CRISPR-Local

Find 468 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATTCTCTTGCTGTTG+TGG	0.505256	8_2:-52271160	Msa1254240:CDS
AAATCAGCAGCACACGTATA+TGG	0.383331	8_2:+52269100	None:intergenic
AACAACTTGACCTCAATGAG+AGG	0.630153	8_2:-52270315	Msa1254240:CDS
AACAAGAACCAGCCATAGGA+AGG	0.559388	8_2:-52269157	Msa1254240:exon
AACTTTGGTTGCTTGTTCTA+GGG	0.482023	8_2:-52269044	Msa1254240:three_prime_UTR
AAGAAGAAAACGAGAATAAC+GGG	0.438031	8_2:+52272884	None:intergenic
AAGAGAAACTACCTCAGCAT+AGG	0.594491	8_2:+52271561	None:intergenic
AAGGAAAGACATTCTCGAAA+TGG	0.454782	8_2:-52269967	Msa1254240:intron
AATCAAGTGGGTCATGGTGG+TGG	0.531920	8_2:-52272482	Msa1254240:CDS
ACAGCTCAATGCACTGTCAG+TGG	0.642330	8_2:-52269558	Msa1254240:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGAAAACGAGAATAAC+GGG	+	chr8_2:52268832-52268851	None:intergenic	30.0%
GAAGAAGAAAACGAGAATAA+CGG	+	chr8_2:52268833-52268852	None:intergenic	30.0%
GTTATGCCAAGAAAATTTCA+AGG	-	chr8_2:52269054-52269073	Msa1254240:three_prime_UTR	30.0%
TGATTGGAACATAAAGGAAA+AGG	-	chr8_2:52269093-52269112	Msa1254240:three_prime_UTR	30.0%
TGAGATTTGTGAAAAGAAAC+AGG	-	chr8_2:52269496-52269515	Msa1254240:intron	30.0%
GGAAACATTTGATCGAAAAT+TGG	-	chr8_2:52269590-52269609	Msa1254240:CDS	30.0%
CACTTGACAATAAAGTAACA+TGG	+	chr8_2:52269644-52269663	None:intergenic	30.0%
AAATTTAATGCCATAGACCA+TGG	-	chr8_2:52269945-52269964	Msa1254240:intron	30.0%
TAACTTGAGTTCATCATCAA+TGG	+	chr8_2:52270314-52270333	None:intergenic	30.0%
TCTTCCTAAAAAGTAGTACA+CGG	+	chr8_2:52270476-52270495	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	52268819	52272916	52268819	ID=Msa1254240;Name=Msa1254240
chr8_2	mRNA	52268819	52272916	52268819	ID=Msa1254240-mRNA-1;Parent=Msa1254240;Name=Msa1254240-mRNA-1;_AED=0.10;_eAED=0.10;_QI=199\|1\|1\|1\|1\|1\|11\|343\|433
chr8_2	exon	52268819	52269312	52268819	ID=Msa1254240-mRNA-1:exon:16004;Parent=Msa1254240-mRNA-1
chr8_2	exon	52269498	52269676	52269498	ID=Msa1254240-mRNA-1:exon:16003;Parent=Msa1254240-mRNA-1
chr8_2	exon	52269772	52269870	52269772	ID=Msa1254240-mRNA-1:exon:16002;Parent=Msa1254240-mRNA-1
chr8_2	exon	52269968	52270189	52269968	ID=Msa1254240-mRNA-1:exon:16001;Parent=Msa1254240-mRNA-1
chr8_2	exon	52270282	52270371	52270282	ID=Msa1254240-mRNA-1:exon:16000;Parent=Msa1254240-mRNA-1
chr8_2	exon	52271119	52271244	52271119	ID=Msa1254240-mRNA-1:exon:15999;Parent=Msa1254240-mRNA-1
chr8_2	exon	52271333	52271473	52271333	ID=Msa1254240-mRNA-1:exon:15998;Parent=Msa1254240-mRNA-1
chr8_2	exon	52271573	52271618	52271573	ID=Msa1254240-mRNA-1:exon:15997;Parent=Msa1254240-mRNA-1
chr8_2	exon	52272189	52272245	52272189	ID=Msa1254240-mRNA-1:exon:15996;Parent=Msa1254240-mRNA-1
chr8_2	exon	52272380	52272512	52272380	ID=Msa1254240-mRNA-1:exon:15995;Parent=Msa1254240-mRNA-1
chr8_2	exon	52272660	52272916	52272660	ID=Msa1254240-mRNA-1:exon:15994;Parent=Msa1254240-mRNA-1
chr8_2	five_prime_UTR	52272718	52272916	52272718	ID=Msa1254240-mRNA-1:five_prime_utr;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52272660	52272717	52272660	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52272380	52272512	52272380	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52272189	52272245	52272189	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52271573	52271618	52271573	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52271333	52271473	52271333	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52271119	52271244	52271119	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52270282	52270371	52270282	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52269968	52270189	52269968	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52269772	52269870	52269772	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52269498	52269676	52269498	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	CDS	52269162	52269312	52269162	ID=Msa1254240-mRNA-1:cds;Parent=Msa1254240-mRNA-1
chr8_2	three_prime_UTR	52268819	52269161	52268819	ID=Msa1254240-mRNA-1:three_prime_utr;Parent=Msa1254240-mRNA-1

Gene Sequence

>Msa1254240

ATGGTGAATACTTCTGAAGAGAACAATTCCAACGCAGTTATGCCAAGAAAATTTCAAGGAGGAATGAATCAAGTGGGTCATGGTGGTGGAAGAATTGTTGGGCAGAACAGAAGAGCTTTAGGAGGGATCAATCAGAATTTTGTGCATGGTCGACCTTACCCTTGTGTTGTACACAAGAGGGTGTTGTCTGAAAAACATGAGATTTGTGAAAAGAAACAGGCTGATCTGGGACATAGACCCATCACAAGGAGGTTTGCTGCACAAATTGCTGGCTCACAGCAATCCTATGCTGAGAAAAAGAATTCAAATCCATTGAATTCGAATGAGTTTGGAAACTCCATCGCCATTGATGATGAACTCAAGTTACCAGAAGACCAACCAGAGCCTATGACTTTGGAGCATACTGAACCAATGCATAGTGATCCACTTGAGATGGAAGAGGTTGAGATGGAGGATATAGAGGGAGAGATGATTTTGGACATTGATAGCTGTGATGCAAACAATTCTCTTGCTGTTGTGGAGTATATTGAAGATCTCCATGCTTACTACAGAAAGATTGAGTATCTTGGCTGTGTTTCACCAACCTACATGGATGAACAACTTGACCTCAATGAGAGGATGAGGGCTATACTAGTTGACTGGCTGATTGAGGTTCATGACAAATTCGACCTCATGCAAGAGACACTGTTTCTTACAGTTAATCTCATAGACAGATTCTTGGCTAAGCAGAATGTTGTAAGAAAGAAACTTCAGCTGGTTGGTCTTGTTGCCATGCTTTTGGCATGCAAGTATGAGGAAGTTTCTGTGCCTGTCGTTTCGGATCTGATCCATATAGCTGACAGAGCTTACACAAGGAAAGACATTCTCGAAATGGAGAAGTCGATGCTCAACACTCTGCAGTATAACATGTCTCTTCCAACTGCTTATGTTTTCATGAGAAGGTTTCTGAAGGCTGCTCAAGCTGACAAAAAACTTGAGCTGGTGGCTTTTTTCTTGGTTGAGCTATCTCTGGTGGAATATGAGATGCTGAAGTTTCCACCATCCTTGGTTGCTGCTGCTGCTGTCTACACAGCTCAATGCACTGTCAGTGGTTTCAAACACTGGAACAAGACATGCGAGTGGCACACAAACTACTCAGAAGATCAGCTGTTAGAGTGCTCTATGCTGATGGTTGGATTTCACCAGAAAGCCGGAGCCGGGAAACTGACGGGAGTGCATAGGAAGTATGGCTCAGCAAAATTTAGCTTCACTGCTAAATGTGAACCAGCATGTTTTCTTCTGGAGAACAAGAACCAGCCATAG

Protein sequence

>Msa1254240

MVNTSEENNSNAVMPRKFQGGMNQVGHGGGRIVGQNRRALGGINQNFVHGRPYPCVVHKRVLSEKHEICEKKQADLGHRPITRRFAAQIAGSQQSYAEKKNSNPLNSNEFGNSIAIDDELKLPEDQPEPMTLEHTEPMHSDPLEMEEVEMEDIEGEMILDIDSCDANNSLAVVEYIEDLHAYYRKIEYLGCVSPTYMDEQLDLNERMRAILVDWLIEVHDKFDLMQETLFLTVNLIDRFLAKQNVVRKKLQLVGLVAMLLACKYEEVSVPVVSDLIHIADRAYTRKDILEMEKSMLNTLQYNMSLPTAYVFMRRFLKAAQADKKLELVAFFLVELSLVEYEMLKFPPSLVAAAAVYTAQCTVSGFKHWNKTCEWHTNYSEDQLLECSMLMVGFHQKAGAGKLTGVHRKYGSAKFSFTAKCEPACFLLENKNQP*