Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067163.t1 | RHN58085.1 | 100 | 62 | 0 | 0 | 1 | 62 | 143 | 204 | 1.90E-25 | 124.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067163.t1 | Q9ZW34 | 81.7 | 60 | 11 | 0 | 1 | 60 | 387 | 446 | 3.2e-21 | 101.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067163.t1 | G7K4W1 | 100.0 | 62 | 0 | 0 | 1 | 62 | 387 | 448 | 1.4e-25 | 124.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene067163 | 0.838703 | 2.46E-57 | -1.69E-46 |
| MS.gene049254 | MS.gene067163 | 0.818876 | 1.56E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene067163 | 0.828509 | 8.64E-55 | -1.69E-46 |
| MS.gene049435 | MS.gene067163 | 0.812661 | 3.79E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene067163 | 0.823313 | 1.48E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene067163 | 0.818503 | 1.89E-52 | -1.69E-46 |
| MS.gene05016 | MS.gene067163 | 0.806859 | 6.70E-50 | -1.69E-46 |
| MS.gene050273 | MS.gene067163 | 0.809167 | 2.16E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene067163 | 0.831718 | 1.42E-55 | -1.69E-46 |
| MS.gene050807 | MS.gene067163 | 0.800238 | 1.58E-48 | -1.69E-46 |
| MS.gene050844 | MS.gene067163 | 0.839812 | 1.27E-57 | -1.69E-46 |
| MS.gene051198 | MS.gene067163 | 0.829752 | 4.32E-55 | -1.69E-46 |
| MS.gene051247 | MS.gene067163 | 0.831702 | 1.44E-55 | -1.69E-46 |
| MS.gene051265 | MS.gene067163 | 0.833712 | 4.56E-56 | -1.69E-46 |
| MS.gene051279 | MS.gene067163 | 0.819769 | 9.76E-53 | -1.69E-46 |
| MS.gene051388 | MS.gene067163 | 0.814868 | 1.24E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene067163 | 0.809479 | 1.85E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene067163 | 0.805205 | 1.49E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene067163 | 0.82547 | 4.61E-54 | -1.69E-46 |
| MS.gene051800 | MS.gene067163 | 0.828881 | 7.02E-55 | -1.69E-46 |
| MS.gene052020 | MS.gene067163 | 0.825956 | 3.53E-54 | -1.69E-46 |
| MS.gene052042 | MS.gene067163 | 0.814297 | 1.66E-51 | -1.69E-46 |
| MS.gene052190 | MS.gene067163 | 0.82125 | 4.46E-53 | -1.69E-46 |
| MS.gene052323 | MS.gene067163 | 0.807373 | 5.22E-50 | -1.69E-46 |
| MS.gene052333 | MS.gene067163 | 0.810314 | 1.23E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene067163 | 0.825903 | 3.64E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene067163 | 0.827403 | 1.59E-54 | -1.69E-46 |
| MS.gene052582 | MS.gene067163 | 0.813173 | 2.93E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene067163 | 0.812441 | 4.23E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene067163 | 0.847497 | 1.11E-59 | -1.69E-46 |
| MS.gene052612 | MS.gene067163 | 0.81731 | 3.52E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene067163 | 0.806702 | 7.24E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene067163 | 0.840138 | 1.04E-57 | -1.69E-46 |
| MS.gene05288 | MS.gene067163 | 0.814702 | 1.35E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene067163 | 0.829766 | 4.28E-55 | -1.69E-46 |
| MS.gene05289 | MS.gene067163 | 0.823173 | 1.60E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene067163 | 0.822096 | 2.84E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene067163 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene053565 | MS.gene067163 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene053584 | MS.gene067163 | 0.817768 | 2.78E-52 | -1.69E-46 |
| MS.gene053621 | MS.gene067163 | 0.807782 | 4.27E-50 | -1.69E-46 |
| MS.gene053967 | MS.gene067163 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene053998 | MS.gene067163 | 0.822023 | 2.96E-53 | -1.69E-46 |
| MS.gene05414 | MS.gene067163 | 0.817526 | 3.15E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene067163 | 0.818682 | 1.73E-52 | -1.69E-46 |
| MS.gene05417 | MS.gene067163 | 0.803901 | 2.79E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene067163 | 0.840245 | 9.77E-58 | -1.69E-46 |
| MS.gene054291 | MS.gene067163 | -0.811721 | 6.08E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene067163 | 0.854014 | 1.63E-61 | -1.69E-46 |
| MS.gene054468 | MS.gene067163 | 0.809867 | 1.53E-50 | -1.69E-46 |
| MS.gene054472 | MS.gene067163 | 0.82298 | 1.77E-53 | -1.69E-46 |
| MS.gene05447 | MS.gene067163 | 0.814865 | 1.24E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene067163 | 0.814558 | 1.45E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene067163 | 0.840483 | 8.46E-58 | -1.69E-46 |
| MS.gene054775 | MS.gene067163 | 0.821255 | 4.45E-53 | -1.69E-46 |
| MS.gene054801 | MS.gene067163 | 0.8091 | 2.24E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene067163 | 0.821238 | 4.49E-53 | -1.69E-46 |
| MS.gene055228 | MS.gene067163 | 0.812408 | 4.30E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene067163 | 0.834038 | 3.78E-56 | -1.69E-46 |
| MS.gene055357 | MS.gene067163 | 0.858016 | 1.10E-62 | -1.69E-46 |
| MS.gene055358 | MS.gene067163 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene067163 | 0.804929 | 1.71E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene067163 | 0.827168 | 1.82E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene067163 | 0.805379 | 1.37E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene067163 | 0.83402 | 3.81E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene067163 | 0.829622 | 4.64E-55 | -1.69E-46 |
| MS.gene05604 | MS.gene067163 | 0.849529 | 3.05E-60 | -1.69E-46 |
| MS.gene056050 | MS.gene067163 | 0.804896 | 1.73E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene067163 | 0.828831 | 7.22E-55 | -1.69E-46 |
| MS.gene056174 | MS.gene067163 | 0.816177 | 6.32E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene067163 | 0.815765 | 7.82E-52 | -1.69E-46 |
| MS.gene056373 | MS.gene067163 | 0.802727 | 4.89E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene067163 | 0.802247 | 6.14E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene067163 | 0.834283 | 3.28E-56 | -1.69E-46 |
| MS.gene056595 | MS.gene067163 | 0.812478 | 4.15E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene067163 | 0.802338 | 5.88E-49 | -1.69E-46 |
| MS.gene057314 | MS.gene067163 | 0.846134 | 2.63E-59 | -1.69E-46 |
| MS.gene057444 | MS.gene067163 | 0.813046 | 3.12E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene067163 | 0.810608 | 1.06E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene067163 | 0.831132 | 1.98E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene067163 | 0.828676 | 7.88E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene067163 | 0.83289 | 7.30E-56 | -1.69E-46 |
| MS.gene058037 | MS.gene067163 | 0.823832 | 1.12E-53 | -1.69E-46 |
| MS.gene058294 | MS.gene067163 | 0.817005 | 4.13E-52 | -1.69E-46 |
| MS.gene058426 | MS.gene067163 | 0.807683 | 4.48E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene067163 | 0.833055 | 6.65E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene067163 | 0.827471 | 1.54E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene067163 | 0.814817 | 1.27E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene067163 | 0.819622 | 1.05E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene067163 | 0.817244 | 3.65E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene067163 | 0.818076 | 2.37E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene067163 | 0.829681 | 4.49E-55 | -1.69E-46 |
| MS.gene059584 | MS.gene067163 | 0.819645 | 1.04E-52 | -1.69E-46 |
| MS.gene059601 | MS.gene067163 | 0.803756 | 3.00E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene067163 | 0.821501 | 3.90E-53 | -1.69E-46 |
| MS.gene059937 | MS.gene067163 | 0.805618 | 1.22E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene067163 | 0.827936 | 1.19E-54 | -1.69E-46 |
| MS.gene060137 | MS.gene067163 | -0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene060167 | MS.gene067163 | 0.807948 | 3.94E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene067163 | 0.803105 | 4.09E-49 | -1.69E-46 |
| MS.gene060312 | MS.gene067163 | 0.830183 | 3.39E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene067163 | 0.845521 | 3.86E-59 | -1.69E-46 |
| MS.gene06052 | MS.gene067163 | 0.827921 | 1.20E-54 | -1.69E-46 |
| MS.gene060781 | MS.gene067163 | 0.800361 | 1.49E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene067163 | 0.802718 | 4.91E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene067163 | 0.804038 | 2.62E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067163.t1 | MTR_5g096970 | 100.000 | 62 | 0 | 0 | 1 | 62 | 387 | 448 | 8.47e-37 | 127 |
| MS.gene067163.t1 | MTR_6g060570 | 73.770 | 61 | 16 | 0 | 1 | 61 | 338 | 398 | 3.23e-26 | 98.6 |
| MS.gene067163.t1 | MTR_7g103620 | 72.131 | 61 | 17 | 0 | 1 | 61 | 353 | 413 | 8.26e-26 | 97.8 |
| MS.gene067163.t1 | MTR_7g103620 | 72.131 | 61 | 17 | 0 | 1 | 61 | 303 | 363 | 6.43e-25 | 94.7 |
| MS.gene067163.t1 | MTR_2g056190 | 72.131 | 61 | 17 | 0 | 1 | 61 | 405 | 465 | 3.89e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067163.t1 | AT2G29560 | 81.667 | 60 | 11 | 0 | 1 | 60 | 387 | 446 | 5.49e-27 | 101 |
| MS.gene067163.t1 | AT2G36530 | 73.770 | 61 | 16 | 0 | 1 | 61 | 353 | 413 | 4.67e-26 | 98.6 |
| MS.gene067163.t1 | AT2G36530 | 73.770 | 61 | 16 | 0 | 1 | 61 | 342 | 402 | 1.19e-24 | 94.4 |
| MS.gene067163.t1 | AT1G74030 | 72.131 | 61 | 17 | 0 | 1 | 61 | 391 | 451 | 4.40e-24 | 93.2 |
Find 0 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGCGATTAGAAAATACAACC+AGG | - | 37937:9709-9728 | MS.gene067163:CDS | 35.0% | |
| ! | TTCATAGCTGATTTATCCGT+TGG | - | 37937:9642-9661 | MS.gene067163:CDS | 35.0% |
| CTTTAATCTGACCAACAGCA+AGG | + | 37937:9668-9687 | MS.gene067163:intergenic | 40.0% | |
| TTTGACCACTTCAATGACCT+CGG | + | 37937:9559-9578 | MS.gene067163:intergenic | 40.0% | |
| GCTGTTGGTCAGATTAAAGC+AGG | - | 37937:9669-9688 | MS.gene067163:CDS | 45.0% | |
| GGTCATTGAAGTGGTCAAAC+AGG | - | 37937:9560-9579 | MS.gene067163:CDS | 45.0% | |
| TGAAGTGGTCAAACAGGCAA+AGG | - | 37937:9566-9585 | MS.gene067163:CDS | 45.0% | |
| GTGGTGACATCTCACAGAAG+TGG | - | 37937:9603-9622 | MS.gene067163:CDS | 50.0% | |
| TCTAATCGCTCTCCTCTGCA+AGG | + | 37937:9699-9718 | MS.gene067163:intergenic | 50.0% | |
| TGTGACCGAGGTCATTGAAG+TGG | - | 37937:9551-9570 | MS.gene067163:CDS | 50.0% | |
| ! | AACAGGCAAAGGAAGCGCAT+TGG | - | 37937:9577-9596 | MS.gene067163:CDS | 50.0% |
| ! | ACAGGCAAAGGAAGCGCATT+GGG | - | 37937:9578-9597 | MS.gene067163:CDS | 50.0% |
| !! | TTATCCGTTGGCCTTGCTGT+TGG | - | 37937:9654-9673 | MS.gene067163:CDS | 50.0% |
| CCTCGGTCACAGTTCCAACT+TGG | + | 37937:9542-9561 | MS.gene067163:intergenic | 55.0% | |
| CTGACCAACAGCAAGGCCAA+CGG | + | 37937:9661-9680 | MS.gene067163:intergenic | 55.0% | |
| ! | AGGCAAAGGAAGCGCATTGG+GGG | - | 37937:9580-9599 | MS.gene067163:CDS | 55.0% |
| ! | CAGGCAAAGGAAGCGCATTG+GGG | - | 37937:9579-9598 | MS.gene067163:CDS | 55.0% |
| ! | CCAAGTTGGAACTGTGACCG+AGG | - | 37937:9539-9558 | MS.gene067163:CDS | 55.0% |
| AAAGCAGGCGCTCCTTGCAG+AGG | - | 37937:9684-9703 | MS.gene067163:CDS | 60.0% | |
| ! | AAAGGAAGCGCATTGGGGGG+TGG | - | 37937:9584-9603 | MS.gene067163:CDS | 60.0% |
| ! | GGCAAAGGAAGCGCATTGGG+GGG | - | 37937:9581-9600 | MS.gene067163:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37937 | gene | 9534 | 9730 | 9534 | ID=MS.gene067163 |
| 37937 | mRNA | 9534 | 9730 | 9534 | ID=MS.gene067163.t1;Parent=MS.gene067163 |
| 37937 | exon | 9534 | 9730 | 9534 | ID=MS.gene067163.t1.exon1;Parent=MS.gene067163.t1 |
| 37937 | CDS | 9534 | 9730 | 9534 | ID=cds.MS.gene067163.t1;Parent=MS.gene067163.t1 |
| Gene Sequence |
| Protein sequence |
