AlfalfaGEDB | MS.gene31419

M. sativa cultivar XinJiangDaYe / MS.gene31419

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31419.t1	KEH39197.1	90.1	415	23	3	1	413	31	429	2.10E-205	724.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31419.t1	Q9ZWA6	49.4	443	163	13	1	397	65	492	3.3e-95	350.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31419.t1	A0A072VBM1	90.1	415	23	3	1	413	31	429	1.5e-205	724.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene31419.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene31419	MS.gene002990	PPI
MS.gene31419	MS.gene02229	PPI
MS.gene31419	MS.gene035805	PPI
MS.gene31419	MS.gene052836	PPI
MS.gene98320	MS.gene31419	PPI
MS.gene31419	MS.gene049090	PPI
MS.gene31419	MS.gene047456	PPI
MS.gene31419	MS.gene015782	PPI
MS.gene31419	MS.gene022490	PPI
MS.gene055771	MS.gene31419	PPI
MS.gene31419	MS.gene012660	PPI
MS.gene35597	MS.gene31419	PPI
MS.gene31419	MS.gene015781	PPI
MS.gene05885	MS.gene31419	PPI
MS.gene31419	MS.gene013049	PPI
MS.gene31419	MS.gene35600	PPI
MS.gene061459	MS.gene31419	PPI
MS.gene31419	MS.gene002268	PPI
MS.gene31419	MS.gene067189	PPI
MS.gene31419	MS.gene01250	PPI
MS.gene31419	MS.gene052375	PPI
MS.gene31419	MS.gene044255	PPI
MS.gene31419	MS.gene25787	PPI
MS.gene31419	MS.gene04728	PPI
MS.gene31419	MS.gene069683	PPI
MS.gene31419	MS.gene041216	PPI
MS.gene31419	MS.gene00834	PPI
MS.gene31419	MS.gene002271	PPI
MS.gene02226	MS.gene31419	PPI
MS.gene31419	MS.gene35598	PPI
MS.gene048829	MS.gene31419	PPI
MS.gene31419	MS.gene02231	PPI
MS.gene31419	MS.gene69038	PPI
MS.gene31419	MS.gene059585	PPI
MS.gene31419	MS.gene047454	PPI
MS.gene31419	MS.gene035091	PPI
MS.gene034713	MS.gene31419	PPI
MS.gene31419	MS.gene047449	PPI
MS.gene31419	MS.gene48433	PPI
MS.gene31419	MS.gene73852	PPI
MS.gene31419	MS.gene02090	PPI
MS.gene31419	MS.gene02232	PPI
MS.gene31419	MS.gene82578	PPI
MS.gene067184	MS.gene31419	PPI
MS.gene31419	MS.gene05447	PPI
MS.gene009212	MS.gene31419	PPI
MS.gene31419	MS.gene042910	PPI
MS.gene31419	MS.gene032645	PPI
MS.gene31419	MS.gene01251	PPI
MS.gene31419	MS.gene042166	PPI
MS.gene31419	MS.gene053073	PPI
MS.gene31419	MS.gene73119	PPI
MS.gene31419	MS.gene35599	PPI
MS.gene31419	MS.gene022192	PPI
MS.gene31419	MS.gene47874	PPI
MS.gene31419	MS.gene69039	PPI
MS.gene31419	MS.gene002273	PPI
MS.gene31419	MS.gene048828	PPI
MS.gene31419	MS.gene059586	PPI
MS.gene31419	MS.gene01252	PPI
MS.gene31419	MS.gene23967	PPI
MS.gene31419	MS.gene29831	PPI
MS.gene026317	MS.gene31419	PPI
MS.gene31419	MS.gene52369	PPI
MS.gene036057	MS.gene31419	PPI
MS.gene31419	MS.gene032641	PPI
MS.gene29823	MS.gene31419	PPI
MS.gene31419	MS.gene01248	PPI
MS.gene31419	MS.gene35594	PPI
MS.gene31419	MS.gene041220	PPI
MS.gene31419	MS.gene52365	PPI
MS.gene038672	MS.gene31419	PPI
MS.gene31419	MS.gene002275	PPI
MS.gene31419	MS.gene69732	PPI
MS.gene31419	MS.gene93974	PPI
MS.gene31419	MS.gene72231	PPI
MS.gene31419	MS.gene30256	PPI
MS.gene31419	MS.gene46427	PPI
MS.gene31419	MS.gene067179	PPI
MS.gene31419	MS.gene038081	PPI
MS.gene31419	MS.gene031671	PPI
MS.gene020412	MS.gene31419	PPI
MS.gene31419	MS.gene71067	PPI
MS.gene31419	MS.gene44555	PPI
MS.gene31419	MS.gene022488	PPI
MS.gene31419	MS.gene022491	PPI
MS.gene52366	MS.gene31419	PPI
MS.gene31419	MS.gene002272	PPI
MS.gene31419	MS.gene74552	PPI
MS.gene31419	MS.gene35602	PPI
MS.gene31419	MS.gene44556	PPI
MS.gene31419	MS.gene031835	PPI
MS.gene31419	MS.gene026307	PPI
MS.gene31419	MS.gene030366	PPI
MS.gene31419	MS.gene05884	PPI
MS.gene31419	MS.gene68947	PPI
MS.gene31419	MS.gene41007	PPI
MS.gene52364	MS.gene31419	PPI
MS.gene31419	MS.gene29826	PPI
MS.gene31419	MS.gene01019	PPI
MS.gene31419	MS.gene035420	PPI
MS.gene022492	MS.gene31419	PPI
MS.gene31419	MS.gene28928	PPI
MS.gene31419	MS.gene049091	PPI
MS.gene31419	MS.gene80830	PPI
MS.gene003290	MS.gene31419	PPI
MS.gene31419	MS.gene030960	PPI
MS.gene31419	MS.gene52371	PPI
MS.gene31419	MS.gene01255	PPI
MS.gene31419	MS.gene52370	PPI
MS.gene31419	MS.gene041679	PPI
MS.gene31419	MS.gene27917	PPI
MS.gene31419	MS.gene032644	PPI
MS.gene31419	MS.gene053074	PPI
MS.gene31419	MS.gene026300	PPI
MS.gene31419	MS.gene053075	PPI
MS.gene31419	MS.gene061460	PPI
MS.gene31419	MS.gene026330	PPI
MS.gene31419	MS.gene27394	PPI
MS.gene31419	MS.gene002270	PPI
MS.gene31419	MS.gene27206	PPI
MS.gene31419	MS.gene43567	PPI
MS.gene31419	MS.gene32947	PPI
MS.gene82809	MS.gene31419	PPI
MS.gene31419	MS.gene067183	PPI
MS.gene31419	MS.gene006634	PPI
MS.gene31419	MS.gene89577	PPI
MS.gene31419	MS.gene003378	PPI
MS.gene31419	MS.gene38844	PPI
MS.gene02356	MS.gene31419	PPI
MS.gene31419	MS.gene29828	PPI
MS.gene31419	MS.gene82811	PPI
MS.gene31419	MS.gene36132	PPI
MS.gene31419	MS.gene03126	PPI
MS.gene31419	MS.gene052174	PPI
MS.gene065927	MS.gene31419	PPI
MS.gene31419	MS.gene047450	PPI
MS.gene31419	MS.gene047453	PPI
MS.gene31419	MS.gene022489	PPI
MS.gene31419	MS.gene82810	PPI
MS.gene31419	MS.gene43577	PPI
MS.gene72613	MS.gene31419	PPI
MS.gene31419	MS.gene98828	PPI
MS.gene31419	MS.gene02230	PPI
MS.gene31419	MS.gene72232	PPI
MS.gene044589	MS.gene31419	PPI
MS.gene31419	MS.gene94332	PPI
MS.gene31419	MS.gene29827	PPI
MS.gene31419	MS.gene01253	PPI
MS.gene31419	MS.gene000498	PPI
MS.gene31419	MS.gene044586	PPI
MS.gene31419	MS.gene000494	PPI
MS.gene31419	MS.gene052854	PPI
MS.gene43565	MS.gene31419	PPI
MS.gene31419	MS.gene047446	PPI
MS.gene31419	MS.gene068992	PPI
MS.gene31419	MS.gene022484	PPI
MS.gene31419	MS.gene43566	PPI
MS.gene31419	MS.gene70542	PPI
MS.gene31419	MS.gene035088	PPI
MS.gene31419	MS.gene68850	PPI
MS.gene31419	MS.gene01895	PPI
MS.gene31419	MS.gene29829	PPI
MS.gene31419	MS.gene54116	PPI
MS.gene31419	MS.gene98322	PPI
MS.gene31419	MS.gene064542	PPI
MS.gene73887	MS.gene31419	PPI
MS.gene67951	MS.gene31419	PPI
MS.gene31419	MS.gene04727	PPI
MS.gene31419	MS.gene02234	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31419.t1	MTR_2g090745	91.807	415	16	3	1	413	31	429	0.0	751
MS.gene31419.t1	MTR_4g059870	49.693	326	118	7	1	320	62	347	2.75e-97	300
MS.gene31419.t1	MTR_2g099990	89.209	139	15	0	1	139	70	208	1.91e-93	289
MS.gene31419.t1	MTR_2g099990	89.209	139	15	0	1	139	1	139	1.93e-93	286
MS.gene31419.t1	MTR_8g017210	89.928	139	14	0	1	139	86	224	9.22e-93	289
MS.gene31419.t1	MTR_1g112270	48.105	343	154	7	1	320	68	409	1.43e-89	282
MS.gene31419.t1	MTR_1g112270	48.105	343	154	7	1	320	79	420	2.57e-89	281
MS.gene31419.t1	MTR_1g094115	84.058	138	22	0	1	138	71	208	5.73e-88	277
MS.gene31419.t1	MTR_1g016010	81.818	143	25	1	1	142	65	207	9.26e-85	266
MS.gene31419.t1	MTR_1g094115	82.836	134	23	0	5	138	58	191	1.76e-84	267
MS.gene31419.t1	MTR_2g093960	69.591	171	42	3	1	170	56	217	5.20e-84	263
MS.gene31419.t1	MTR_2g017720	90.099	101	5	1	1	101	337	432	4.27e-58	196
MS.gene31419.t1	MTR_3g102000	78.788	99	14	2	4	98	36	131	5.78e-48	163
MS.gene31419.t1	MTR_1g093095	33.557	149	86	5	1	137	110	257	1.18e-16	80.5
MS.gene31419.t1	MTR_5g030560	93.548	31	2	0	1	31	30	60	4.00e-15	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31419.t1	AT1G03840	49.436	443	163	13	1	397	63	490	7.44e-119	355
MS.gene31419.t1	AT1G03840	49.436	443	163	13	1	397	65	492	9.72e-119	355
MS.gene31419.t1	AT5G44160	48.966	435	141	14	1	397	22	413	3.11e-116	346
MS.gene31419.t1	AT5G44160	49.766	428	148	14	1	397	61	452	3.25e-116	347
MS.gene31419.t1	AT2G02080	46.705	349	139	10	1	326	1	325	1.53e-92	286
MS.gene31419.t1	AT2G02080	46.705	349	139	10	1	326	1	325	1.53e-92	286
MS.gene31419.t1	AT2G02080	46.991	349	138	10	1	326	78	402	2.78e-92	287
MS.gene31419.t1	AT2G02080	46.991	349	138	10	1	326	78	402	2.78e-92	287
MS.gene31419.t1	AT2G02080	46.991	349	138	10	1	326	78	402	2.78e-92	287
MS.gene31419.t1	AT2G02080	46.991	349	138	10	1	326	78	402	2.78e-92	287
MS.gene31419.t1	AT5G66730	87.050	139	18	0	1	139	56	194	5.53e-92	286
MS.gene31419.t1	AT5G03150	43.439	442	173	15	1	401	77	482	1.07e-90	283
MS.gene31419.t1	AT4G02670	80.405	148	28	1	1	147	79	226	8.22e-90	278
MS.gene31419.t1	AT4G02670	80.405	148	28	1	1	147	77	224	9.10e-90	277
MS.gene31419.t1	AT1G55110	85.714	140	19	1	1	139	87	226	4.28e-89	277
MS.gene31419.t1	AT1G55110	85.714	140	19	1	1	139	87	226	4.28e-89	277
MS.gene31419.t1	AT1G55110	85.714	140	19	1	1	139	87	226	4.28e-89	277
MS.gene31419.t1	AT3G45260	49.693	326	131	11	1	316	63	365	4.28e-89	277
MS.gene31419.t1	AT3G45260	49.693	326	131	11	1	316	63	365	4.28e-89	277
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	75	214	4.18e-88	276
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	94	233	4.64e-88	276
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	94	233	4.64e-88	276
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	89	228	5.78e-88	276
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	81	220	7.71e-88	275
MS.gene31419.t1	AT3G13810	87.857	140	16	1	1	139	95	234	1.16e-87	275
MS.gene31419.t1	AT2G02070	81.295	139	26	0	1	139	76	214	1.06e-86	275
MS.gene31419.t1	AT2G02070	81.295	139	26	0	1	139	76	214	1.06e-86	275
MS.gene31419.t1	AT1G14580	80.576	139	27	0	1	139	77	215	2.54e-86	270
MS.gene31419.t1	AT1G14580	80.576	139	27	0	1	139	77	215	2.54e-86	270
MS.gene31419.t1	AT1G14580	80.576	139	27	0	1	139	91	229	4.44e-86	270
MS.gene31419.t1	AT5G60470	75.177	141	34	1	1	140	59	199	8.65e-81	256
MS.gene31419.t1	AT5G60470	75.177	141	34	1	1	140	68	208	1.46e-80	255
MS.gene31419.t1	AT5G60470	75.177	141	34	1	1	140	64	204	1.59e-80	255
MS.gene31419.t1	AT1G68130	65.152	132	44	2	1	130	65	196	4.55e-58	196
MS.gene31419.t1	AT1G25250	63.910	133	45	2	1	130	34	166	3.33e-57	192
MS.gene31419.t1	AT1G25250	63.910	133	45	2	1	130	57	189	3.69e-57	192
MS.gene31419.t1	AT2G01940	62.687	134	48	2	1	132	68	201	1.07e-56	193
MS.gene31419.t1	AT2G01940	61.481	135	49	3	1	132	68	202	7.92e-53	182
MS.gene31419.t1	AT1G68130	64.545	110	37	2	23	130	1	110	6.05e-45	159
MS.gene31419.t1	AT1G25250	63.063	111	38	2	23	130	1	111	1.12e-43	155
MS.gene31419.t1	AT2G01940	62.500	112	40	2	23	132	1	112	1.30e-43	156
MS.gene31419.t1	AT5G22890	35.000	140	77	4	1	126	212	351	1.33e-19	90.1
MS.gene31419.t1	AT1G34370	34.965	143	79	3	1	129	239	381	3.25e-19	90.1
MS.gene31419.t1	AT1G34370	34.965	143	79	3	1	129	239	381	3.25e-19	90.1
MS.gene31419.t1	AT1G34370	34.965	143	79	3	1	129	29	171	3.46e-19	87.8
MS.gene31419.t1	AT1G13290	34.337	166	87	9	1	154	96	251	7.45e-18	84.0
MS.gene31419.t1	AT1G34790	36.842	133	74	5	6	129	145	276	1.01e-17	83.6
MS.gene31419.t1	AT3G20880	34.783	138	80	5	1	129	251	387	2.46e-17	84.0
MS.gene31419.t1	AT1G51220	33.333	138	82	5	1	129	173	309	6.04e-17	82.0
MS.gene31419.t1	AT1G08290	37.500	136	72	6	5	129	179	312	2.02e-16	80.5
MS.gene31419.t1	AT3G57670	33.813	139	80	6	1	129	212	348	3.12e-16	80.1

Find 70 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGATCAGGTTTATCTCTT+TGG	0.192108	2.4:+9690654	MS.gene31419:CDS
GAAGAAACAGCTAGAATAAA+TGG	0.212216	2.4:+9690473	MS.gene31419:CDS
TGTATAGTGCAAATGAAAAT+TGG	0.226846	2.4:+9690768	MS.gene31419:CDS
CAATCATCCTTCAAGGGCTT+TGG	0.260521	2.4:+9689495	MS.gene31419:CDS
AATATAAACAACTCATTATT+AGG	0.262088	2.4:+9690503	MS.gene31419:CDS
CTCTTGACATCCATTATTAG+AGG	0.287901	2.4:-9690810	None:intergenic
AATAAATTCTCCTCTAATAA+TGG	0.294626	2.4:+9690800	MS.gene31419:CDS
CTGCATGCGACGCCGTTTCA+TGG	0.296065	2.4:-9691191	None:intergenic
GATCTAGATGTTTGATTAGT+TGG	0.313962	2.4:-9690638	None:intergenic
AAAGAACAAGTAAAGAAATA+AGG	0.321457	2.4:+9689434	MS.gene31419:CDS
TAATAACATCGATAATAATA+AGG	0.327167	2.4:+9690865	MS.gene31419:CDS
TCGATGTTATTATTAACTAA+TGG	0.340481	2.4:-9690854	None:intergenic
TGCAGAATGAAGAGAGCGTT+TGG	0.365539	2.4:+9691209	MS.gene31419:CDS
TCAGCAGAGAGCTACATTTC+AGG	0.365625	2.4:+9691148	MS.gene31419:CDS
GCAAATGAAAATTGGGTGTT+TGG	0.367718	2.4:+9690776	MS.gene31419:CDS
GTGTGTGAAATTTGTCTAAA+GGG	0.373283	2.4:+9689346	MS.gene31419:CDS
TCATTATTCCTTGGAAGCTT+AGG	0.378657	2.4:+9691034	MS.gene31419:CDS
GTACTTACAACAAGTGATCT+TGG	0.379692	2.4:+9691124	MS.gene31419:CDS
TCTTGATTTGTGCATGAGAT+TGG	0.386882	2.4:-9690614	None:intergenic
AATCATCCTTCAAGGGCTTT+GGG	0.387364	2.4:+9689496	MS.gene31419:CDS
AGGGCTTTGGGAGATTTAAC+TGG	0.390376	2.4:+9689508	MS.gene31419:CDS
TGCATGCGACGCCGTTTCAT+GGG	0.403719	2.4:-9691190	None:intergenic
TTTGATGATGAGATTGTGAT+TGG	0.412049	2.4:-9690940	None:intergenic
GCAGAATGAAGAGAGCGTTT+GGG	0.418512	2.4:+9691210	MS.gene31419:CDS
GACAAATTTCACACACGAAT+CGG	0.419935	2.4:-9689339	None:intergenic
CGTGTGTGAAATTTGTCTAA+AGG	0.423777	2.4:+9689345	MS.gene31419:CDS
TCTAGCTGTTTCTTCTGCTA+AGG	0.433712	2.4:-9690465	None:intergenic
TTTACGCTGTTCAATCGGAT+TGG	0.439019	2.4:+9689596	MS.gene31419:CDS
GTGTTCACAATCATCCTTCA+AGG	0.445387	2.4:+9689488	MS.gene31419:CDS
TCAACTGAATCATTATTCCT+TGG	0.446740	2.4:+9691025	MS.gene31419:CDS
AACAACTCATTATTAGGAAT+GGG	0.451735	2.4:+9690509	MS.gene31419:CDS
AAATCTCCCAAAGCCCTTGA+AGG	0.460893	2.4:-9689502	None:intergenic
GACGTGGACATAACCTTCCT+TGG	0.468137	2.4:+9689401	MS.gene31419:CDS
TTGTGAACACATGTCTTCTC+TGG	0.471074	2.4:-9689475	None:intergenic
AAACAACTCATTATTAGGAA+TGG	0.474295	2.4:+9690508	MS.gene31419:CDS
AGAGCGTTTGGGGACAAACT+AGG	0.488786	2.4:+9691221	MS.gene31419:CDS
ATGCATGAGATTCATCAACT+TGG	0.490859	2.4:+9690722	MS.gene31419:CDS
GTTCTTTGCTTCAGCTTCCA+AGG	0.495209	2.4:-9689418	None:intergenic
TACTTACAACAAGTGATCTT+GGG	0.495492	2.4:+9691125	MS.gene31419:CDS
GTATAGTGCAAATGAAAATT+GGG	0.498164	2.4:+9690769	MS.gene31419:CDS
AATAGTCCAAGTCAAGATCA+TGG	0.501314	2.4:+9691067	MS.gene31419:CDS
GGAAGCACGGTGAGAAGAAG+TGG	0.504824	2.4:+9689551	MS.gene31419:CDS
GAGCGTTTGGGGACAAACTA+GGG	0.510953	2.4:+9691222	MS.gene31419:CDS
GGGATAGCTTTGTCACTCAC+AGG	0.519723	2.4:+9690429	MS.gene31419:CDS
TTTGCTTCAGCTTCCAAGGA+AGG	0.519934	2.4:-9689414	None:intergenic
ACTAATCAAACATCTAGATC+AGG	0.520793	2.4:+9690641	MS.gene31419:CDS
AATTCTCTTGTCCCAAACAT+AGG	0.523465	2.4:+9690566	MS.gene31419:CDS
TTGTTCCCATGATCTTGACT+TGG	0.537836	2.4:-9691073	None:intergenic
GCATGCGACGCCGTTTCATG+GGG	0.540481	2.4:-9691189	None:intergenic
CTTGGGTCCGCTACAAGCAC+AGG	0.540913	2.4:+9690740	MS.gene31419:CDS
GATTGATGAACTGTGGCAAA+TGG	0.542982	2.4:-9691270	None:intergenic
ATCTCAAACCTAAGCTTCCA+AGG	0.557018	2.4:-9691042	None:intergenic
TGCATGAGATTCATCAACTT+GGG	0.577843	2.4:+9690723	MS.gene31419:CDS
AGAGAATACAAATGTGATTG+TGG	0.583936	2.4:+9689646	MS.gene31419:CDS
GGAATGGGAGTTAACAACAT+TGG	0.588635	2.4:+9690524	MS.gene31419:CDS
TGCCACAGTTCATCAATCAA+CGG	0.590617	2.4:+9691275	MS.gene31419:CDS
TGTTCACAATCATCCTTCAA+GGG	0.591498	2.4:+9689489	MS.gene31419:CDS
ATGCGACGCCGTTTCATGGG+GGG	0.603099	2.4:-9691187	None:intergenic
ATAGTCCAAGTCAAGATCAT+GGG	0.604288	2.4:+9691068	MS.gene31419:CDS
CAGAATGAAGAGAGCGTTTG+GGG	0.617026	2.4:+9691211	MS.gene31419:CDS
CATTGCTCCTGTGCTTGTAG+CGG	0.619229	2.4:-9690747	None:intergenic
ACAGTTCATCAATCAACGGA+TGG	0.619902	2.4:+9691279	MS.gene31419:CDS
CATGCGACGCCGTTTCATGG+GGG	0.637741	2.4:-9691188	None:intergenic
AAAGCTCACTCAAAGACTTG+TGG	0.644093	2.4:+9689619	MS.gene31419:CDS
TGTTGCTGACATGTTTGCAG+AGG	0.649923	2.4:-9690966	None:intergenic
TTGGTGTTGTGAGCTATACA+AGG	0.662064	2.4:-9690921	None:intergenic
TGTTGTGAGCTATACAAGGA+AGG	0.666542	2.4:-9690917	None:intergenic
GGATAGCTTTGTCACTCACA+GGG	0.679845	2.4:+9690430	MS.gene31419:CDS
AATCTTCAACTTCATCGACG+TGG	0.715188	2.4:+9689385	MS.gene31419:CDS
ATCCGTTGATTGATGAACTG+TGG	0.722662	2.4:-9691277	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTAACAATAAACAAAAAA+TGG	-	chr2.4:9690378-9690397	None:intergenic	10.0%
!!	AATTACTTATCAATATAATT+TGG	+	chr2.4:9690394-9690413	MS.gene31419:intron	10.0%
!!	ATTAACAATAAACAAAAAAT+GGG	-	chr2.4:9690377-9690396	None:intergenic	10.0%
!!	AAATAGGTAATAAACTTAAA+TGG	-	chr2.4:9689792-9689811	None:intergenic	15.0%
!!	AATATAAACAACTCATTATT+AGG	+	chr2.4:9690503-9690522	MS.gene31419:CDS	15.0%
!!	TAATAACATCGATAATAATA+AGG	+	chr2.4:9690865-9690884	MS.gene31419:CDS	15.0%
!!	TGTTTATATTTAAGAATAGA+AGG	+	chr2.4:9690290-9690309	MS.gene31419:intron	15.0%
!!	TTGTATGGAATAAAATAAAA+GGG	+	chr2.4:9689962-9689981	MS.gene31419:intron	15.0%
!!!	GTTTTTCTTTCTAAATATAA+CGG	+	chr2.4:9690056-9690075	MS.gene31419:intron	15.0%
!!	AAAGAACAAGTAAAGAAATA+AGG	+	chr2.4:9689434-9689453	MS.gene31419:CDS	20.0%
!!	AATAAATTCTCCTCTAATAA+TGG	+	chr2.4:9690800-9690819	MS.gene31419:CDS	20.0%
!!	AATAATAATCACAAAAGGAA+AGG	-	chr2.4:9689887-9689906	None:intergenic	20.0%
!!	ACAGAAATAATAATCACAAA+AGG	-	chr2.4:9689892-9689911	None:intergenic	20.0%
!!	CAAAAATGTACATTTACATA+TGG	-	chr2.4:9690272-9690291	None:intergenic	20.0%
!!	CTTGTATGGAATAAAATAAA+AGG	+	chr2.4:9689961-9689980	MS.gene31419:intron	20.0%
!!	TCGATGTTATTATTAACTAA+TGG	-	chr2.4:9690857-9690876	None:intergenic	20.0%
!	AAAAATGAGTTCCTATGTTT+GGG	-	chr2.4:9690580-9690599	None:intergenic	25.0%
!	AAACAACTCATTATTAGGAA+TGG	+	chr2.4:9690508-9690527	MS.gene31419:CDS	25.0%
!	AACAACTCATTATTAGGAAT+GGG	+	chr2.4:9690509-9690528	MS.gene31419:CDS	25.0%
!	AATTACTTTGACTAATACGA+GGG	+	chr2.4:9689915-9689934	MS.gene31419:intron	25.0%
!	GAATAAAATAAAAGGGTCTT+GGG	+	chr2.4:9689969-9689988	MS.gene31419:intron	25.0%
!	GTATAGTGCAAATGAAAATT+GGG	+	chr2.4:9690769-9690788	MS.gene31419:CDS	25.0%
!	TAATTACTTTGACTAATACG+AGG	+	chr2.4:9689914-9689933	MS.gene31419:intron	25.0%
!	TACTACTCTTTAATCTTAAG+TGG	-	chr2.4:9690104-9690123	None:intergenic	25.0%
!	TGTATAGTGCAAATGAAAAT+TGG	+	chr2.4:9690768-9690787	MS.gene31419:CDS	25.0%
!!	AAAATTTTACGCTGTTCAAT+CGG	+	chr2.4:9689591-9689610	MS.gene31419:CDS	25.0%
!!	TTGAAAATAGAAGTCTTGTA+TGG	+	chr2.4:9689947-9689966	MS.gene31419:intron	25.0%
!!!	ATTTCTAGCTACTAATAGTT+TGG	+	chr2.4:9689834-9689853	MS.gene31419:intron	25.0%
!!!	ATTTGTCTATTGCTCTAAAA+TGG	+	chr2.4:9689749-9689768	MS.gene31419:intron	25.0%
	AAAGTAGTGTGAAGTCTAAT+TGG	+	chr2.4:9690123-9690142	MS.gene31419:intron	30.0%
	ACTAATCAAACATCTAGATC+AGG	+	chr2.4:9690641-9690660	MS.gene31419:CDS	30.0%
	AGAGAATACAAATGTGATTG+TGG	+	chr2.4:9689646-9689665	MS.gene31419:CDS	30.0%
	AGCTACTAATAGTTTGGATT+TGG	+	chr2.4:9689840-9689859	MS.gene31419:intron	30.0%
	GAAAAATGAGTTCCTATGTT+TGG	-	chr2.4:9690581-9690600	None:intergenic	30.0%
	GAAGAAACAGCTAGAATAAA+TGG	+	chr2.4:9690473-9690492	MS.gene31419:CDS	30.0%
	GATCTAGATGTTTGATTAGT+TGG	-	chr2.4:9690641-9690660	None:intergenic	30.0%
	GGAATAAAATAAAAGGGTCT+TGG	+	chr2.4:9689968-9689987	MS.gene31419:intron	30.0%
	GTGTGTGAAATTTGTCTAAA+GGG	+	chr2.4:9689346-9689365	MS.gene31419:CDS	30.0%
	TACTTACAACAAGTGATCTT+GGG	+	chr2.4:9691125-9691144	MS.gene31419:CDS	30.0%
	TCAACTGAATCATTATTCCT+TGG	+	chr2.4:9691025-9691044	MS.gene31419:CDS	30.0%
!	ATCATGGGAACAAATTTTGT+GGG	+	chr2.4:9691083-9691102	MS.gene31419:CDS	30.0%
!	CGTTTAAGATAACTGCAAAT+AGG	-	chr2.4:9689808-9689827	None:intergenic	30.0%
!	TTGTCTAAAGGGTTTTCAAA+GGG	+	chr2.4:9689357-9689376	MS.gene31419:CDS	30.0%
!	TTTGTCTAAAGGGTTTTCAA+AGG	+	chr2.4:9689356-9689375	MS.gene31419:CDS	30.0%
!!	AATATAATTTGGTGCAACAC+AGG	+	chr2.4:9690405-9690424	MS.gene31419:intron	30.0%
!!	TTTGATGATGAGATTGTGAT+TGG	-	chr2.4:9690943-9690962	None:intergenic	30.0%
!!!	GTATAAAGAAGCACTTTTGT+AGG	+	chr2.4:9689530-9689549	MS.gene31419:CDS	30.0%
	AAGTGTAAACAAACCCCTAT+TGG	-	chr2.4:9690212-9690231	None:intergenic	35.0%
	AATAGTCCAAGTCAAGATCA+TGG	+	chr2.4:9691067-9691086	MS.gene31419:CDS	35.0%
	AATTCTCTTGTCCCAAACAT+AGG	+	chr2.4:9690566-9690585	MS.gene31419:CDS	35.0%
	ATAGTCCAAGTCAAGATCAT+GGG	+	chr2.4:9691068-9691087	MS.gene31419:CDS	35.0%
	ATGCATGAGATTCATCAACT+TGG	+	chr2.4:9690722-9690741	MS.gene31419:CDS	35.0%
	CGTGTGTGAAATTTGTCTAA+AGG	+	chr2.4:9689345-9689364	MS.gene31419:CDS	35.0%
	CTAGATCAGGTTTATCTCTT+TGG	+	chr2.4:9690654-9690673	MS.gene31419:CDS	35.0%
	CTCTTGACATCCATTATTAG+AGG	-	chr2.4:9690813-9690832	None:intergenic	35.0%
	GACAAATTTCACACACGAAT+CGG	-	chr2.4:9689342-9689361	None:intergenic	35.0%
	GCAAATGAAAATTGGGTGTT+TGG	+	chr2.4:9690776-9690795	MS.gene31419:CDS	35.0%
	GTACTTACAACAAGTGATCT+TGG	+	chr2.4:9691124-9691143	MS.gene31419:CDS	35.0%
	TCATTATTCCTTGGAAGCTT+AGG	+	chr2.4:9691034-9691053	MS.gene31419:CDS	35.0%
	TGCAAAAAGCTGCTCAAATT+GGG	+	chr2.4:9690996-9691015	MS.gene31419:CDS	35.0%
	TGCATGAGATTCATCAACTT+GGG	+	chr2.4:9690723-9690742	MS.gene31419:CDS	35.0%
	TGTTCACAATCATCCTTCAA+GGG	+	chr2.4:9689489-9689508	MS.gene31419:CDS	35.0%
	TTGCAAAAAGCTGCTCAAAT+TGG	+	chr2.4:9690995-9691014	MS.gene31419:CDS	35.0%
!	AACAAATTTTGTGGGGTGTA+TGG	+	chr2.4:9691091-9691110	MS.gene31419:CDS	35.0%
!	ATTGTGGCACTATCTTTTCA+AGG	+	chr2.4:9689662-9689681	MS.gene31419:CDS	35.0%
!	GATCATGGGAACAAATTTTG+TGG	+	chr2.4:9691082-9691101	MS.gene31419:CDS	35.0%
!	TCATGGGAACAAATTTTGTG+GGG	+	chr2.4:9691084-9691103	MS.gene31419:CDS	35.0%
!!	TCTTGATTTGTGCATGAGAT+TGG	-	chr2.4:9690617-9690636	None:intergenic	35.0%
	AAAGCTCACTCAAAGACTTG+TGG	+	chr2.4:9689619-9689638	MS.gene31419:CDS	40.0%
	AATCATCCTTCAAGGGCTTT+GGG	+	chr2.4:9689496-9689515	MS.gene31419:CDS	40.0%
	AATCTTCAACTTCATCGACG+TGG	+	chr2.4:9689385-9689404	MS.gene31419:CDS	40.0%
	ACAGTTCATCAATCAACGGA+TGG	+	chr2.4:9691279-9691298	MS.gene31419:CDS	40.0%
	ACGAAGTATATAGTGCTCGA+AGG	+	chr2.4:9689998-9690017	MS.gene31419:intron	40.0%
	ATCTCAAACCTAAGCTTCCA+AGG	-	chr2.4:9691045-9691064	None:intergenic	40.0%
	CCGACGCTAAAATTCCAATA+GGG	+	chr2.4:9690195-9690214	MS.gene31419:intron	40.0%
	CGAAGTATATAGTGCTCGAA+GGG	+	chr2.4:9689999-9690018	MS.gene31419:intron	40.0%
	CGACGCTAAAATTCCAATAG+GGG	+	chr2.4:9690196-9690215	MS.gene31419:intron	40.0%
	GAGCACTATATACTTCGTTG+AGG	-	chr2.4:9689996-9690015	None:intergenic	40.0%
	GATTGATGAACTGTGGCAAA+TGG	-	chr2.4:9691273-9691292	None:intergenic	40.0%
	GGAATGGGAGTTAACAACAT+TGG	+	chr2.4:9690524-9690543	MS.gene31419:CDS	40.0%
	GTGTTCACAATCATCCTTCA+AGG	+	chr2.4:9689488-9689507	MS.gene31419:CDS	40.0%
	TGCCACAGTTCATCAATCAA+CGG	+	chr2.4:9691275-9691294	MS.gene31419:CDS	40.0%
	TGTTGTGAGCTATACAAGGA+AGG	-	chr2.4:9690920-9690939	None:intergenic	40.0%
	TTGTGAACACATGTCTTCTC+TGG	-	chr2.4:9689478-9689497	None:intergenic	40.0%
	TTGTTCCCATGATCTTGACT+TGG	-	chr2.4:9691076-9691095	None:intergenic	40.0%
	TTTACGCTGTTCAATCGGAT+TGG	+	chr2.4:9689596-9689615	MS.gene31419:CDS	40.0%
!	AAGCACTTTTGTAGGAAGCA+CGG	+	chr2.4:9689538-9689557	MS.gene31419:CDS	40.0%
!	ATCCGTTGATTGATGAACTG+TGG	-	chr2.4:9691280-9691299	None:intergenic	40.0%
!	GGACAAACTAGGGATTTTCT+TGG	+	chr2.4:9691232-9691251	MS.gene31419:CDS	40.0%
!!	ACTAGGGATTTTCTTGGTGT+TGG	+	chr2.4:9691238-9691257	MS.gene31419:CDS	40.0%
!!	ATAATTTGGTGCAACACAGG+AGG	+	chr2.4:9690408-9690427	MS.gene31419:intron	40.0%
!!	CCCTATTGGAATTTTAGCGT+CGG	-	chr2.4:9690198-9690217	None:intergenic	40.0%
!!	TAATTTGGTGCAACACAGGA+GGG	+	chr2.4:9690409-9690428	MS.gene31419:intron	40.0%
!!	TCTAGCTGTTTCTTCTGCTA+AGG	-	chr2.4:9690468-9690487	None:intergenic	40.0%
!!	TTGGTGTTGTGAGCTATACA+AGG	-	chr2.4:9690924-9690943	None:intergenic	40.0%
!!!	AATTTTAGCGTCGGCTCATT+TGG	-	chr2.4:9690189-9690208	None:intergenic	40.0%
	AAATCTCCCAAAGCCCTTGA+AGG	-	chr2.4:9689505-9689524	None:intergenic	45.0%
	CAATCATCCTTCAAGGGCTT+TGG	+	chr2.4:9689495-9689514	MS.gene31419:CDS	45.0%
	GCCGACGCTAAAATTCCAAT+AGG	+	chr2.4:9690194-9690213	MS.gene31419:intron	45.0%
	GGTTCTCTAGCTACTATTGC+AGG	+	chr2.4:9690077-9690096	MS.gene31419:intron	45.0%
	GTTCTTTGCTTCAGCTTCCA+AGG	-	chr2.4:9689421-9689440	None:intergenic	45.0%
	TCAGCAGAGAGCTACATTTC+AGG	+	chr2.4:9691148-9691167	MS.gene31419:CDS	45.0%
	TGTTGCTGACATGTTTGCAG+AGG	-	chr2.4:9690969-9690988	None:intergenic	45.0%
	TTTGCTTCAGCTTCCAAGGA+AGG	-	chr2.4:9689417-9689436	None:intergenic	45.0%
!!	AGGGCTTTGGGAGATTTAAC+TGG	+	chr2.4:9689508-9689527	MS.gene31419:CDS	45.0%
!!	CAGAATGAAGAGAGCGTTTG+GGG	+	chr2.4:9691211-9691230	MS.gene31419:CDS	45.0%
!!	GCAGAATGAAGAGAGCGTTT+GGG	+	chr2.4:9691210-9691229	MS.gene31419:CDS	45.0%
!!	GGATAGCTTTGTCACTCACA+GGG	+	chr2.4:9690430-9690449	MS.gene31419:CDS	45.0%
!!	TGCAGAATGAAGAGAGCGTT+TGG	+	chr2.4:9691209-9691228	MS.gene31419:CDS	45.0%
!!!	AGGGATTTTCTTGGTGTTGG+TGG	+	chr2.4:9691241-9691260	MS.gene31419:CDS	45.0%
	CACAAATGCCCCCCATGAAA+CGG	+	chr2.4:9691179-9691198	MS.gene31419:CDS	50.0%
	CATTGCTCCTGTGCTTGTAG+CGG	-	chr2.4:9690750-9690769	None:intergenic	50.0%
	GACGTGGACATAACCTTCCT+TGG	+	chr2.4:9689401-9689420	MS.gene31419:CDS	50.0%
	GAGCGTTTGGGGACAAACTA+GGG	+	chr2.4:9691222-9691241	MS.gene31419:CDS	50.0%
!	AGAGCGTTTGGGGACAAACT+AGG	+	chr2.4:9691221-9691240	MS.gene31419:CDS	50.0%
!!	GGGATAGCTTTGTCACTCAC+AGG	+	chr2.4:9690429-9690448	MS.gene31419:CDS	50.0%
	TGCATGCGACGCCGTTTCAT+GGG	-	chr2.4:9691193-9691212	None:intergenic	55.0%
!	GGAAGCACGGTGAGAAGAAG+TGG	+	chr2.4:9689551-9689570	MS.gene31419:CDS	55.0%
	ATGCGACGCCGTTTCATGGG+GGG	-	chr2.4:9691190-9691209	None:intergenic	60.0%
	CATGCGACGCCGTTTCATGG+GGG	-	chr2.4:9691191-9691210	None:intergenic	60.0%
	CTGCATGCGACGCCGTTTCA+TGG	-	chr2.4:9691194-9691213	None:intergenic	60.0%
	CTTGGGTCCGCTACAAGCAC+AGG	+	chr2.4:9690740-9690759	MS.gene31419:CDS	60.0%
	GCATGCGACGCCGTTTCATG+GGG	-	chr2.4:9691192-9691211	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	9689328	9691312	9689328	ID=MS.gene31419
chr2.4	mRNA	9689328	9691312	9689328	ID=MS.gene31419.t1;Parent=MS.gene31419
chr2.4	exon	9689328	9689683	9689328	ID=MS.gene31419.t1.exon1;Parent=MS.gene31419.t1
chr2.4	CDS	9689328	9689683	9689328	ID=cds.MS.gene31419.t1;Parent=MS.gene31419.t1
chr2.4	exon	9690427	9691312	9690427	ID=MS.gene31419.t1.exon2;Parent=MS.gene31419.t1
chr2.4	CDS	9690427	9691312	9690427	ID=cds.MS.gene31419.t1;Parent=MS.gene31419.t1

Gene Sequence

>MS.gene31419

ATGGCAACAAACCGATTCGTGTGTGAAATTTGTCTAAAGGGTTTTCAAAGGGATCAAAATCTTCAACTTCATCGACGTGGACATAACCTTCCTTGGAAGCTGAAGCAAAGAACAAGTAAAGAAATAAGGAAGAGAGTGTATGTGTGTCCAGAGAAGACATGTGTTCACAATCATCCTTCAAGGGCTTTGGGAGATTTAACTGGTATAAAGAAGCACTTTTGTAGGAAGCACGGTGAGAAGAAGTGGAAGTGTGAGAAGTGCTCAAAATTTTACGCTGTTCAATCGGATTGGAAAGCTCACTCAAAGACTTGTGGCACTAGAGAATACAAATGTGATTGTGGCACTATCTTTTCAAGGTAAGTAATTTATCTTTTCTTAATTCTTAGATGTCATACTCAAAAAAATTTATGTCAAAGTTGTAATTTGTCTATTGCTCTAAAATGGATCATCATTGAAAGAATCCATTTAAGTTTATTACCTATTTGCAGTTATCTTAAACGACAAAAATTTCTAGCTACTAATAGTTTGGATTTGGAATTATCTAATTATTAATCTACCTTTCCTTTTGTGATTATTATTTCTGTTTTAATTACTTTGACTAATACGAGGGTTGAGATTGTTGAAAATAGAAGTCTTGTATGGAATAAAATAAAAGGGTCTTGGGTCCTCAACGAAGTATATAGTGCTCGAAGGGATCGTAACATTTAGAGAAAAAAACAACTCAATTAGTTTTTCTTTCTAAATATAACGGTTCTCTAGCTACTATTGCAGGACCACTTAAGATTAAAGAGTAGTAAAGTAGTGTGAAGTCTAATTGGATAAATTATTTTAGATCATACTAATATTCAATAACACTTTCCAAATGAGCCGACGCTAAAATTCCAATAGGGGTTTGTTTACACTTAATCTTAACTATAAGCAAAAATACACATTTATTTAGTCCATATGTAAATGTACATTTTTGTTTATATTTAAGAATAGAAGGAGTACAAGTTATGACACTTACAAGCTAGTCATCATGTTATACTAATTTTCTTACATTAATTCCCATTTTTTGTTTATTGTTAATTACTTATCAATATAATTTGGTGCAACACAGGAGGGATAGCTTTGTCACTCACAGGGCATTCTGTGATGCCTTAGCAGAAGAAACAGCTAGAATAAATGGATCAGCAAATATAAACAACTCATTATTAGGAATGGGAGTTAACAACATTGGTAATTACAACATCATGCAAAATTCTCTTGTCCCAAACATAGGAACTCATTTTTCTTCAATTTTCAAACCAATCTCATGCACAAATCAAGAACCAACTAATCAAACATCTAGATCAGGTTTATCTCTTTGGATGTCTCAAACATCTCATGACACAATATTGACTAATAATAGCAATATGCATGAGATTCATCAACTTGGGTCCGCTACAAGCACAGGAGCAATGTATAGTGCAAATGAAAATTGGGTGTTTGGAAATAAATTCTCCTCTAATAATGGATGTCAAGAGTTAACTAGTTCTGTTTCACTTCCATTAGTTAATAATAACATCGATAATAATAAGGATAGTACTACAAACAATGTTGTTAGTGTTCCTTCCTTGTATAGCTCACAACACCAATCACAATCTCATCATCAAACAACCTCTGCAAACATGTCAGCAACAGCTTTGTTGCAAAAAGCTGCTCAAATTGGGACAACTTCAACTGAATCATTATTCCTTGGAAGCTTAGGTTTGAGATGCAATAGTCCAAGTCAAGATCATGGGAACAAATTTTGTGGGGTGTATGGTTCAAGTTCAGTACTTACAACAAGTGATCTTGGGTCAGCAGAGAGCTACATTTCAGGTGAATTGTCACAAATGCCCCCCATGAAACGGCGTCGCATGCAGAATGAAGAGAGCGTTTGGGGACAAACTAGGGATTTTCTTGGTGTTGGTGGTGCTAACCATTTGCCACAGTTCATCAATCAACGGATGGATTTGATTTGA

Protein sequence

>MS.gene31419.t1

MATNRFVCEICLKGFQRDQNLQLHRRGHNLPWKLKQRTSKEIRKRVYVCPEKTCVHNHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHSKTCGTREYKCDCGTIFSRRDSFVTHRAFCDALAEETARINGSANINNSLLGMGVNNIGNYNIMQNSLVPNIGTHFSSIFKPISCTNQEPTNQTSRSGLSLWMSQTSHDTILTNNSNMHEIHQLGSATSTGAMYSANENWVFGNKFSSNNGCQELTSSVSLPLVNNNIDNNKDSTTNNVVSVPSLYSSQHQSQSHHQTTSANMSATALLQKAAQIGTTSTESLFLGSLGLRCNSPSQDHGNKFCGVYGSSSVLTTSDLGSAESYISGELSQMPPMKRRRMQNEESVWGQTRDFLGVGGANHLPQFINQRMDLI