Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08577.t1 | XP_003606501.2 | 97.7 | 609 | 14 | 0 | 1 | 609 | 1 | 609 | 4.50E-289 | 1003.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08577.t1 | Q9FII7 | 53.4 | 607 | 281 | 1 | 5 | 609 | 3 | 609 | 4.6e-173 | 609.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08577.t1 | G7JRH6 | 97.7 | 609 | 14 | 0 | 1 | 609 | 1 | 609 | 3.2e-289 | 1003.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene08577 | 0.841339 | 5.05E-58 | -1.69E-46 |
| MS.gene049159 | MS.gene08577 | 0.849282 | 3.57E-60 | -1.69E-46 |
| MS.gene049254 | MS.gene08577 | 0.825435 | 4.70E-54 | -1.69E-46 |
| MS.gene049368 | MS.gene08577 | 0.811652 | 6.29E-51 | -1.69E-46 |
| MS.gene049404 | MS.gene08577 | 0.823401 | 1.41E-53 | -1.69E-46 |
| MS.gene049435 | MS.gene08577 | 0.841872 | 3.65E-58 | -1.69E-46 |
| MS.gene049585 | MS.gene08577 | 0.805488 | 1.30E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene08577 | 0.814567 | 1.44E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene08577 | 0.809671 | 1.69E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene08577 | 0.908588 | 1.45E-81 | -1.69E-46 |
| MS.gene050020 | MS.gene08577 | 0.808321 | 3.28E-50 | -1.69E-46 |
| MS.gene050273 | MS.gene08577 | 0.809011 | 2.34E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene08577 | 0.830105 | 3.54E-55 | -1.69E-46 |
| MS.gene050559 | MS.gene08577 | 0.803877 | 2.83E-49 | -1.69E-46 |
| MS.gene050561 | MS.gene08577 | 0.810619 | 1.05E-50 | -1.69E-46 |
| MS.gene050562 | MS.gene08577 | 0.804941 | 1.70E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene08577 | 0.831688 | 1.45E-55 | -1.69E-46 |
| MS.gene050807 | MS.gene08577 | 0.83084 | 2.34E-55 | -1.69E-46 |
| MS.gene050808 | MS.gene08577 | 0.802819 | 4.68E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene08577 | 0.800319 | 1.52E-48 | -1.69E-46 |
| MS.gene050962 | MS.gene08577 | 0.828669 | 7.91E-55 | -1.69E-46 |
| MS.gene051102 | MS.gene08577 | 0.805634 | 1.21E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene08577 | 0.846674 | 1.87E-59 | -1.69E-46 |
| MS.gene051247 | MS.gene08577 | 0.830324 | 3.13E-55 | -1.69E-46 |
| MS.gene051279 | MS.gene08577 | 0.853569 | 2.19E-61 | -1.69E-46 |
| MS.gene051490 | MS.gene08577 | 0.810591 | 1.07E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene08577 | 0.84032 | 9.34E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene08577 | 0.843093 | 1.73E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene08577 | 0.854762 | 9.92E-62 | -1.69E-46 |
| MS.gene051744 | MS.gene08577 | 0.856055 | 4.17E-62 | -1.69E-46 |
| MS.gene051800 | MS.gene08577 | 0.848149 | 7.37E-60 | -1.69E-46 |
| MS.gene052020 | MS.gene08577 | 0.827817 | 1.27E-54 | -1.69E-46 |
| MS.gene05215 | MS.gene08577 | 0.808975 | 2.38E-50 | -1.69E-46 |
| MS.gene052239 | MS.gene08577 | -0.800836 | 1.20E-48 | -1.69E-46 |
| MS.gene052307 | MS.gene08577 | 0.804675 | 1.93E-49 | -1.69E-46 |
| MS.gene052311 | MS.gene08577 | 0.820591 | 6.33E-53 | -1.69E-46 |
| MS.gene052323 | MS.gene08577 | 0.822731 | 2.03E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene08577 | 0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene08577 | 0.827901 | 1.21E-54 | -1.69E-46 |
| MS.gene052582 | MS.gene08577 | 0.832582 | 8.70E-56 | -1.69E-46 |
| MS.gene052584 | MS.gene08577 | 0.828328 | 9.56E-55 | -1.69E-46 |
| MS.gene052609 | MS.gene08577 | 0.847418 | 1.17E-59 | -1.69E-46 |
| MS.gene052611 | MS.gene08577 | 0.806453 | 8.17E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene08577 | 0.846628 | 1.93E-59 | -1.69E-46 |
| MS.gene052773 | MS.gene08577 | 0.826651 | 2.41E-54 | -1.69E-46 |
| MS.gene052775 | MS.gene08577 | 0.828484 | 8.76E-55 | -1.69E-46 |
| MS.gene052776 | MS.gene08577 | 0.81856 | 1.84E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene08577 | 0.805248 | 1.46E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene08577 | 0.811012 | 8.66E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene08577 | 0.832689 | 8.19E-56 | -1.69E-46 |
| MS.gene053144 | MS.gene08577 | 0.821046 | 4.97E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene08577 | 0.847777 | 9.33E-60 | -1.69E-46 |
| MS.gene053296 | MS.gene08577 | 0.821719 | 3.48E-53 | -1.69E-46 |
| MS.gene053621 | MS.gene08577 | 0.839464 | 1.56E-57 | -1.69E-46 |
| MS.gene05364 | MS.gene08577 | 0.828701 | 7.77E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene08577 | 0.817373 | 3.41E-52 | -1.69E-46 |
| MS.gene054236 | MS.gene08577 | 0.803503 | 3.38E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene08577 | 0.813795 | 2.14E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene08577 | 0.814419 | 1.56E-51 | -1.69E-46 |
| MS.gene05447 | MS.gene08577 | 0.812842 | 3.46E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene08577 | 0.817655 | 2.95E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene08577 | 0.839987 | 1.14E-57 | -1.69E-46 |
| MS.gene05462 | MS.gene08577 | 0.802153 | 6.42E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene08577 | 0.850121 | 2.08E-60 | -1.69E-46 |
| MS.gene05468 | MS.gene08577 | 0.805902 | 1.07E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene08577 | 0.809796 | 1.58E-50 | -1.69E-46 |
| MS.gene054824 | MS.gene08577 | 0.800276 | 1.55E-48 | -1.69E-46 |
| MS.gene055144 | MS.gene08577 | 0.807056 | 6.09E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene08577 | 0.831663 | 1.47E-55 | -1.69E-46 |
| MS.gene055228 | MS.gene08577 | 0.812888 | 3.38E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene08577 | 0.846174 | 2.56E-59 | -1.69E-46 |
| MS.gene055466 | MS.gene08577 | 0.836953 | 6.92E-57 | -1.69E-46 |
| MS.gene055468 | MS.gene08577 | 0.809606 | 1.74E-50 | -1.69E-46 |
| MS.gene055469 | MS.gene08577 | 0.840177 | 1.02E-57 | -1.69E-46 |
| MS.gene055512 | MS.gene08577 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene08577 | 0.827906 | 1.21E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene08577 | 0.804503 | 2.09E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene08577 | 0.813729 | 2.21E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene08577 | 0.83353 | 5.06E-56 | -1.69E-46 |
| MS.gene05604 | MS.gene08577 | 0.814223 | 1.72E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene08577 | 0.842556 | 2.41E-58 | -1.69E-46 |
| MS.gene056173 | MS.gene08577 | 0.839789 | 1.28E-57 | -1.69E-46 |
| MS.gene056174 | MS.gene08577 | 0.83272 | 8.05E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene08577 | 0.855955 | 4.46E-62 | -1.69E-46 |
| MS.gene056366 | MS.gene08577 | 0.821887 | 3.18E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene08577 | 0.88615 | 4.39E-72 | -1.69E-46 |
| MS.gene056553 | MS.gene08577 | 0.835232 | 1.89E-56 | -1.69E-46 |
| MS.gene056622 | MS.gene08577 | 0.825476 | 4.59E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene08577 | 0.820075 | 8.31E-53 | -1.69E-46 |
| MS.gene057034 | MS.gene08577 | 0.808757 | 2.65E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene08577 | 0.806443 | 8.21E-50 | -1.69E-46 |
| MS.gene057294 | MS.gene08577 | 0.813982 | 1.94E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene08577 | 0.811413 | 7.09E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene08577 | 0.817705 | 2.87E-52 | -1.69E-46 |
| MS.gene057446 | MS.gene08577 | 0.832002 | 1.21E-55 | -1.69E-46 |
| MS.gene057598 | MS.gene08577 | 0.822243 | 2.63E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene08577 | 0.803646 | 3.16E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene08577 | 0.888541 | 5.39E-73 | -1.69E-46 |
| MS.gene057915 | MS.gene08577 | 0.865249 | 6.77E-65 | -1.69E-46 |
| MS.gene058010 | MS.gene08577 | 0.843509 | 1.34E-58 | -1.69E-46 |
| MS.gene058289 | MS.gene08577 | 0.823468 | 1.36E-53 | -1.69E-46 |
| MS.gene058330 | MS.gene08577 | 0.805791 | 1.13E-49 | -1.69E-46 |
| MS.gene05847 | MS.gene08577 | 0.8182 | 2.22E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene08577 | 0.831133 | 1.98E-55 | -1.69E-46 |
| MS.gene058647 | MS.gene08577 | 0.841563 | 4.41E-58 | -1.69E-46 |
| MS.gene058649 | MS.gene08577 | 0.808025 | 3.79E-50 | -1.69E-46 |
| MS.gene058919 | MS.gene08577 | 0.840358 | 9.13E-58 | -1.69E-46 |
| MS.gene058979 | MS.gene08577 | 0.818837 | 1.59E-52 | -1.69E-46 |
| MS.gene059331 | MS.gene08577 | 0.824563 | 7.54E-54 | -1.69E-46 |
| MS.gene059335 | MS.gene08577 | 0.822869 | 1.88E-53 | -1.69E-46 |
| MS.gene059439 | MS.gene08577 | 0.849212 | 3.74E-60 | -1.69E-46 |
| MS.gene059570 | MS.gene08577 | 0.84165 | 4.18E-58 | -1.69E-46 |
| MS.gene059725 | MS.gene08577 | 0.845088 | 5.05E-59 | -1.69E-46 |
| MS.gene05978 | MS.gene08577 | 0.82055 | 6.47E-53 | -1.69E-46 |
| MS.gene059842 | MS.gene08577 | 0.80437 | 2.23E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene08577 | 0.813279 | 2.77E-51 | -1.69E-46 |
| MS.gene060312 | MS.gene08577 | 0.81652 | 5.30E-52 | -1.69E-46 |
| MS.gene060373 | MS.gene08577 | 0.8072 | 5.68E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene08577 | 0.809059 | 2.28E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene08577 | 0.841154 | 5.64E-58 | -1.69E-46 |
| MS.gene060587 | MS.gene08577 | 0.840306 | 9.41E-58 | -1.69E-46 |
| MS.gene060637 | MS.gene08577 | 0.808593 | 2.87E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene08577 | 0.809135 | 2.20E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene08577 | 0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene08577 | 0.808452 | 3.08E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08577.t1 | MTR_4g061050 | 97.701 | 609 | 14 | 0 | 1 | 609 | 1 | 609 | 0.0 | 1234 |
| MS.gene08577.t1 | MTR_4g036760 | 77.673 | 636 | 127 | 2 | 1 | 621 | 1 | 636 | 0.0 | 998 |
| MS.gene08577.t1 | MTR_1g018410 | 60.294 | 68 | 21 | 2 | 159 | 224 | 8 | 71 | 5.40e-15 | 70.9 |
| MS.gene08577.t1 | MTR_5g036370 | 21.940 | 629 | 409 | 22 | 21 | 622 | 22 | 595 | 4.13e-12 | 70.1 |
| MS.gene08577.t1 | MTR_4g115050 | 23.038 | 395 | 241 | 13 | 22 | 403 | 11 | 355 | 2.09e-11 | 67.8 |
| MS.gene08577.t1 | MTR_3g033470 | 21.859 | 398 | 250 | 12 | 1 | 386 | 1 | 349 | 2.88e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08577.t1 | AT5G07660 | 57.699 | 591 | 248 | 1 | 21 | 609 | 20 | 610 | 0.0 | 707 |
| MS.gene08577.t1 | AT5G61460 | 56.837 | 607 | 260 | 1 | 5 | 609 | 3 | 609 | 0.0 | 680 |
| MS.gene08577.t1 | AT5G15920 | 24.922 | 321 | 193 | 11 | 13 | 318 | 12 | 299 | 3.02e-12 | 70.5 |
Find 115 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGATTATACTTCCATTT+TGG | 0.225795 | 4.1:-38203927 | None:intergenic |
| CATACGAGTCTAGTTCTTCT+AGG | 0.228029 | 4.1:+38203794 | MS.gene08577:CDS |
| ATTGATGGATGGTTTGTATT+AGG | 0.237226 | 4.1:-38217282 | None:intergenic |
| TATATTTACTTTCATGATCT+TGG | 0.256816 | 4.1:-38216081 | None:intergenic |
| ATAACATCACCATATATTTC+AGG | 0.282347 | 4.1:-38205527 | None:intergenic |
| ATTTCATTAAAACTGGTTCT+AGG | 0.288257 | 4.1:+38205059 | MS.gene08577:CDS |
| GTAAAGACCTCCTTCAGATT+TGG | 0.299303 | 4.1:-38218606 | None:intergenic |
| TGCCTCCCATTGGTCCAATT+GGG | 0.304442 | 4.1:+38216305 | MS.gene08577:CDS |
| ATGCCTCCCATTGGTCCAAT+TGG | 0.309677 | 4.1:+38216304 | MS.gene08577:CDS |
| GAATAACAAAGATAAATTTA+AGG | 0.322875 | 4.1:+38208066 | MS.gene08577:CDS |
| TTGAAGGATTTCATTAAAAC+TGG | 0.329937 | 4.1:+38205052 | MS.gene08577:CDS |
| AATCTCATCACTGGCCAAAA+TGG | 0.333927 | 4.1:+38203913 | MS.gene08577:CDS |
| AGAGATGTCATCAAAGGTTT+AGG | 0.334447 | 4.1:+38216281 | MS.gene08577:CDS |
| TTCCTCCATGTCAGATTCTT+CGG | 0.343251 | 4.1:-38215588 | None:intergenic |
| GGGAAGCAGATCACTGCTTT+TGG | 0.344550 | 4.1:+38216223 | MS.gene08577:intron |
| TAAAGACCTCCTTCAGATTT+GGG | 0.344785 | 4.1:-38218605 | None:intergenic |
| CATTAATGACCAACATTGAT+AGG | 0.346514 | 4.1:+38215772 | MS.gene08577:CDS |
| CTTGTTAACGACTATGGTAT+TGG | 0.346686 | 4.1:+38218555 | MS.gene08577:CDS |
| TGAACTTCTTGAAAGCATTT+CGG | 0.351113 | 4.1:+38208711 | MS.gene08577:CDS |
| TGTAATAGTTGAAGATTTAG+AGG | 0.367142 | 4.1:+38208753 | MS.gene08577:CDS |
| TGGTCCAATTGGGGCCCATC+TGG | 0.379616 | 4.1:+38216315 | MS.gene08577:CDS |
| GCTTTCAAGCCTGAAATATA+TGG | 0.382189 | 4.1:+38205518 | MS.gene08577:CDS |
| TGTAATAGAGCTTGAGGATT+CGG | 0.389954 | 4.1:-38205570 | None:intergenic |
| CGGCTGAGTTTGAATGGAAA+AGG | 0.396255 | 4.1:+38215642 | MS.gene08577:CDS |
| CACCGTGCTTAATGTCTTAG+TGG | 0.401602 | 4.1:+38217332 | MS.gene08577:CDS |
| GATCATGAAAGTAAATATAA+TGG | 0.407899 | 4.1:+38216085 | MS.gene08577:CDS |
| AAGTGGTTGCCTTTGAGAAC+AGG | 0.409368 | 4.1:+38218580 | MS.gene08577:CDS |
| TCATTGAGTTCTTTCTCTAT+AGG | 0.409841 | 4.1:-38208787 | None:intergenic |
| TTGTTAACGACTATGGTATT+GGG | 0.410665 | 4.1:+38218556 | MS.gene08577:CDS |
| AAAAGAAACTTGCATGGTCA+TGG | 0.425831 | 4.1:+38208875 | MS.gene08577:CDS |
| GCAAAGAGCATCTACTTTGA+AGG | 0.428223 | 4.1:+38205036 | MS.gene08577:CDS |
| GTTTAACAGAAGTTGCATAA+TGG | 0.430183 | 4.1:+38216966 | MS.gene08577:intron |
| GGTATGGTTATTCAAGTTCC+CGG | 0.437661 | 4.1:+38218655 | MS.gene08577:CDS |
| CAAGTGCTTGTTAACGACTA+TGG | 0.438425 | 4.1:+38218549 | MS.gene08577:CDS |
| TGTAAGATGGAAAGAATTGA+TGG | 0.438951 | 4.1:-38217297 | None:intergenic |
| TCAATCCTAGCTTGGCATAC+AGG | 0.440880 | 4.1:-38208964 | None:intergenic |
| ACTGCGTTGTGTGTTGCATT+TGG | 0.443216 | 4.1:+38204995 | MS.gene08577:CDS |
| TATACCAGATGGGCCCCAAT+TGG | 0.450039 | 4.1:-38216319 | None:intergenic |
| GCATTTGGTTGTCGAGCTAA+AGG | 0.450992 | 4.1:+38205010 | MS.gene08577:CDS |
| AGCTAGGATTGATAAACAAC+TGG | 0.458878 | 4.1:+38208975 | MS.gene08577:CDS |
| ATTGAGTTTCGTGATTTGAA+TGG | 0.458958 | 4.1:-38203876 | None:intergenic |
| GGATGCCAAAATTAAAATGA+TGG | 0.466285 | 4.1:+38208813 | MS.gene08577:CDS |
| AATAAGCAGCATTGCTGCTA+AGG | 0.472056 | 4.1:+38215806 | MS.gene08577:CDS |
| GCTTAATGTCTTAGTGGATC+AGG | 0.472279 | 4.1:+38217338 | MS.gene08577:CDS |
| TGGGCCCCAATTGGACCAAT+GGG | 0.477585 | 4.1:-38216310 | None:intergenic |
| TGTGCAAAGGAAGCAGACTA+TGG | 0.478040 | 4.1:+38217083 | MS.gene08577:CDS |
| ATGAATAATGAACAAGGGTA+TGG | 0.481370 | 4.1:+38218639 | MS.gene08577:CDS |
| TCAAATCACGAAACTCAATT+CGG | 0.483466 | 4.1:+38203880 | MS.gene08577:CDS |
| CGACTATGGTATTGGGAAAG+TGG | 0.483512 | 4.1:+38218563 | MS.gene08577:CDS |
| GTTGTTTATCAATCCTAGCT+TGG | 0.484038 | 4.1:-38208972 | None:intergenic |
| ATGATTCCTGCTTCAAGAGA+TGG | 0.484469 | 4.1:-38203823 | None:intergenic |
| TTGATGCGGCTGAGTTTGAA+TGG | 0.487145 | 4.1:+38215636 | MS.gene08577:CDS |
| GTATTAGGACGCATGCGCTC+TGG | 0.488270 | 4.1:-38217267 | None:intergenic |
| AAAGCTTGAACTACAAATGC+AGG | 0.494805 | 4.1:+38213546 | MS.gene08577:CDS |
| GTTGCTGTTGAATATGCCAT+TGG | 0.501678 | 4.1:+38217002 | MS.gene08577:CDS |
| TTTCAATGTAATAGAGCTTG+AGG | 0.504415 | 4.1:-38205576 | None:intergenic |
| GACCAACATTGATAGGCAAA+AGG | 0.508262 | 4.1:+38215779 | MS.gene08577:CDS |
| CCATGTTGAAATTCAAAATG+AGG | 0.509978 | 4.1:+38205487 | MS.gene08577:CDS |
| GCCTCCCATTGGTCCAATTG+GGG | 0.510794 | 4.1:+38216306 | MS.gene08577:CDS |
| AGAGAAAGAACTCAATGAGC+TGG | 0.512786 | 4.1:+38208792 | MS.gene08577:CDS |
| GAAGAAACTTGAGGATCAAA+GGG | 0.513256 | 4.1:+38208912 | MS.gene08577:CDS |
| GATTGAAAAGCTGAAGAGTC+GGG | 0.514690 | 4.1:+38208939 | MS.gene08577:CDS |
| ATACGAGTCTAGTTCTTCTA+GGG | 0.516297 | 4.1:+38203795 | MS.gene08577:CDS |
| AGCTAGTAATGTGCAGAAGA+TGG | 0.517397 | 4.1:+38213510 | MS.gene08577:CDS |
| ATGGGCCCCAATTGGACCAA+TGG | 0.518253 | 4.1:-38216311 | None:intergenic |
| AAAGAAACTTGCATGGTCAT+GGG | 0.520535 | 4.1:+38208876 | MS.gene08577:CDS |
| GTCATTCTCGCATTGTAAGA+TGG | 0.521018 | 4.1:-38217310 | None:intergenic |
| GTTTCGCCATCTCTTGAAGC+AGG | 0.522455 | 4.1:+38203817 | MS.gene08577:CDS |
| ATCACAAGTTAAGCAAATGA+AGG | 0.529664 | 4.1:+38213282 | MS.gene08577:CDS |
| AGAAGAAACTTGAGGATCAA+AGG | 0.531514 | 4.1:+38208911 | MS.gene08577:CDS |
| TTTAAGCCACTCTTTGTCAT+CGG | 0.538730 | 4.1:+38213309 | MS.gene08577:CDS |
| TGTTGAACGAATTGCTGTAC+AGG | 0.549951 | 4.1:+38208840 | MS.gene08577:CDS |
| TGAAATTCAAAATGAGGGAG+AGG | 0.550279 | 4.1:+38205493 | MS.gene08577:CDS |
| GGGTTCCTGTATGCCAAGCT+AGG | 0.551572 | 4.1:+38208959 | MS.gene08577:CDS |
| AATTAAGCGAAAGCTACTCT+AGG | 0.554701 | 4.1:+38213230 | MS.gene08577:CDS |
| TTGCACATTGTTTCAGAAGA+CGG | 0.559966 | 4.1:-38217067 | None:intergenic |
| AACTTGAGGATCAAAGGGTA+AGG | 0.562768 | 4.1:+38208917 | MS.gene08577:CDS |
| TTGCTGTTGAATATGCCATT+GGG | 0.563173 | 4.1:+38217003 | MS.gene08577:CDS |
| AGTTGCATAATGGTAATAAG+TGG | 0.563930 | 4.1:+38216976 | MS.gene08577:CDS |
| CATGTTGAAATTCAAAATGA+GGG | 0.566745 | 4.1:+38205488 | MS.gene08577:CDS |
| AGCAACGTTAATCTCATCAC+TGG | 0.568244 | 4.1:+38203904 | MS.gene08577:CDS |
| AAGGAGGTCTTTACTGTGGA+GGG | 0.570452 | 4.1:+38218615 | MS.gene08577:CDS |
| ACTGAAGGGGCTTCGAGAGG+AGG | 0.585025 | 4.1:+38215613 | MS.gene08577:CDS |
| GGATTGAAAAGCTGAAGAGT+CGG | 0.585532 | 4.1:+38208938 | MS.gene08577:CDS |
| ATTTGGGATCCTGTTCTCAA+AGG | 0.590460 | 4.1:-38218589 | None:intergenic |
| TAATTGAGAGATGTCATCAA+AGG | 0.596749 | 4.1:+38216275 | MS.gene08577:CDS |
| ATTGACTTGCTGAAGAAGTG+TGG | 0.597751 | 4.1:-38208689 | None:intergenic |
| GAGGGTATGAATAATGAACA+AGG | 0.598157 | 4.1:+38218633 | MS.gene08577:CDS |
| GTTGCATAATGGTAATAAGT+GGG | 0.604938 | 4.1:+38216977 | MS.gene08577:CDS |
| TGATGTGGAGAAGAAACTTG+AGG | 0.612008 | 4.1:+38208903 | MS.gene08577:CDS |
| AGGTTTAGGATGCCTCCCAT+TGG | 0.614688 | 4.1:+38216295 | MS.gene08577:CDS |
| GCTTCGAGAGGAGGTTGATG+CGG | 0.616385 | 4.1:+38215622 | MS.gene08577:CDS |
| TCTTGACTCATAATCACACA+CGG | 0.617166 | 4.1:-38208016 | None:intergenic |
| GAAGGAGGTCTTTACTGTGG+AGG | 0.619267 | 4.1:+38218614 | MS.gene08577:CDS |
| AAGCTTGAACTACAAATGCA+GGG | 0.633856 | 4.1:+38213547 | MS.gene08577:CDS |
| TCTTCTGAAACAATGTGCAA+AGG | 0.634559 | 4.1:+38217070 | MS.gene08577:CDS |
| CATCAAAGACTATACCAGAT+GGG | 0.636949 | 4.1:-38216329 | None:intergenic |
| GCCCCAATTGGACCAATGGG+AGG | 0.639867 | 4.1:-38216307 | None:intergenic |
| AGAATTGAACAATCTCCCAA+TGG | 0.643670 | 4.1:-38217018 | None:intergenic |
| AGATGGAAAGAATTGATGGA+TGG | 0.643792 | 4.1:-38217293 | None:intergenic |
| AACATACCGATGACAAAGAG+TGG | 0.646147 | 4.1:-38213315 | None:intergenic |
| GAACAGGATCCCAAATCTGA+AGG | 0.651910 | 4.1:+38218596 | MS.gene08577:CDS |
| ATCCACTAAGACATTAAGCA+CGG | 0.662373 | 4.1:-38217334 | None:intergenic |
| AAAACTGAAGGGGCTTCGAG+AGG | 0.666772 | 4.1:+38215610 | MS.gene08577:CDS |
| ATGTTATCATTGTAGAACGT+AGG | 0.666781 | 4.1:+38205543 | MS.gene08577:CDS |
| CAGGATCCCAAATCTGAAGG+AGG | 0.669794 | 4.1:+38218599 | MS.gene08577:CDS |
| GCAGGCCGAAGAATCTGACA+TGG | 0.672118 | 4.1:+38215583 | MS.gene08577:intron |
| ATGGTCATGGGTTTATGATG+TGG | 0.673471 | 4.1:+38208888 | MS.gene08577:CDS |
| TCTGAAGGAGGTCTTTACTG+TGG | 0.676412 | 4.1:+38218611 | MS.gene08577:CDS |
| AGGGTATGAATAATGAACAA+GGG | 0.679887 | 4.1:+38218634 | MS.gene08577:CDS |
| CTTGACTCATAATCACACAC+GGG | 0.680254 | 4.1:-38208015 | None:intergenic |
| TCAACGACAACAAAGCAACA+AGG | 0.680584 | 4.1:+38216132 | MS.gene08577:CDS |
| GGCCGAAGAATCTGACATGG+AGG | 0.698329 | 4.1:+38215586 | MS.gene08577:intron |
| GCATAATGGTAATAAGTGGG+CGG | 0.698969 | 4.1:+38216980 | MS.gene08577:CDS |
| ATTACATTGAAAGATCAACA+AGG | 0.744632 | 4.1:+38205587 | MS.gene08577:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 38203775 | 38218680 | 38203775 | ID=MS.gene08577 |
| chr4.1 | mRNA | 38203775 | 38218680 | 38203775 | ID=MS.gene08577.t1;Parent=MS.gene08577 |
| chr4.1 | exon | 38203775 | 38203937 | 38203775 | ID=MS.gene08577.t1.exon1;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38203775 | 38203937 | 38203775 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38204975 | 38205080 | 38204975 | ID=MS.gene08577.t1.exon2;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38204975 | 38205080 | 38204975 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38205475 | 38205608 | 38205475 | ID=MS.gene08577.t1.exon3;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38205475 | 38205608 | 38205475 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38207604 | 38207659 | 38207604 | ID=MS.gene08577.t1.exon4;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38207604 | 38207659 | 38207604 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38208001 | 38208087 | 38208001 | ID=MS.gene08577.t1.exon5;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38208001 | 38208087 | 38208001 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38208676 | 38208996 | 38208676 | ID=MS.gene08577.t1.exon6;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38208676 | 38208996 | 38208676 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38213217 | 38213330 | 38213217 | ID=MS.gene08577.t1.exon7;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38213217 | 38213330 | 38213217 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38213466 | 38213600 | 38213466 | ID=MS.gene08577.t1.exon8;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38213466 | 38213600 | 38213466 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38215587 | 38215663 | 38215587 | ID=MS.gene08577.t1.exon9;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38215587 | 38215663 | 38215587 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38215752 | 38215827 | 38215752 | ID=MS.gene08577.t1.exon10;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38215752 | 38215827 | 38215752 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38216079 | 38216153 | 38216079 | ID=MS.gene08577.t1.exon11;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38216079 | 38216153 | 38216079 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38216232 | 38216336 | 38216232 | ID=MS.gene08577.t1.exon12;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38216232 | 38216336 | 38216232 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38216975 | 38217144 | 38216975 | ID=MS.gene08577.t1.exon13;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38216975 | 38217144 | 38216975 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38217257 | 38217359 | 38217257 | ID=MS.gene08577.t1.exon14;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38217257 | 38217359 | 38217257 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| chr4.1 | exon | 38218534 | 38218680 | 38218534 | ID=MS.gene08577.t1.exon15;Parent=MS.gene08577.t1 |
| chr4.1 | CDS | 38218534 | 38218680 | 38218534 | ID=cds.MS.gene08577.t1;Parent=MS.gene08577.t1 |
| Gene Sequence |
| Protein sequence |
