Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06821.t1 | XP_003602380.1 | 85.6 | 506 | 19 | 2 | 1 | 452 | 1 | 506 | 1.50E-230 | 808.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06821.t1 | Q9FK23 | 53.8 | 411 | 189 | 1 | 28 | 438 | 80 | 489 | 6.1e-119 | 429.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06821.t1 | G7J8Z8 | 85.6 | 506 | 19 | 2 | 1 | 452 | 1 | 506 | 1.1e-230 | 808.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene06821.t1 | TR | mTERF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene06821 | 0.81636 | 5.76E-52 | -1.69E-46 |
| MS.gene04933 | MS.gene06821 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene06821 | 0.807649 | 4.56E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene06821 | 0.874725 | 5.39E-68 | -1.69E-46 |
| MS.gene049661 | MS.gene06821 | 0.841468 | 4.67E-58 | -1.69E-46 |
| MS.gene049662 | MS.gene06821 | 0.833795 | 4.34E-56 | -1.69E-46 |
| MS.gene049681 | MS.gene06821 | 0.812214 | 4.74E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene06821 | 0.851185 | 1.05E-60 | -1.69E-46 |
| MS.gene050076 | MS.gene06821 | 0.835357 | 1.76E-56 | -1.69E-46 |
| MS.gene050231 | MS.gene06821 | 0.810993 | 8.74E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene06821 | 0.880337 | 5.97E-70 | -1.69E-46 |
| MS.gene050397 | MS.gene06821 | 0.830985 | 2.16E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene06821 | 0.803895 | 2.80E-49 | -1.69E-46 |
| MS.gene050464 | MS.gene06821 | 0.827305 | 1.68E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene06821 | 0.809978 | 1.45E-50 | -1.69E-46 |
| MS.gene050558 | MS.gene06821 | 0.834409 | 3.05E-56 | -1.69E-46 |
| MS.gene050650 | MS.gene06821 | 0.800875 | 1.17E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene06821 | 0.815741 | 7.92E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene06821 | 0.802282 | 6.04E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene06821 | 0.866583 | 2.56E-65 | -1.69E-46 |
| MS.gene051471 | MS.gene06821 | 0.845645 | 3.57E-59 | -1.69E-46 |
| MS.gene051582 | MS.gene06821 | 0.844334 | 8.06E-59 | -1.69E-46 |
| MS.gene051662 | MS.gene06821 | 0.82555 | 4.41E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene06821 | 0.839991 | 1.14E-57 | -1.69E-46 |
| MS.gene051860 | MS.gene06821 | 0.848482 | 5.96E-60 | -1.69E-46 |
| MS.gene051877 | MS.gene06821 | 0.809231 | 2.10E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene06821 | -0.821924 | 3.12E-53 | -1.69E-46 |
| MS.gene052078 | MS.gene06821 | 0.840478 | 8.49E-58 | -1.69E-46 |
| MS.gene052178 | MS.gene06821 | 0.806408 | 8.35E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene06821 | 0.814867 | 1.24E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene06821 | 0.826235 | 3.03E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene06821 | 0.844348 | 7.99E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene06821 | 0.812119 | 4.98E-51 | -1.69E-46 |
| MS.gene052322 | MS.gene06821 | 0.804257 | 2.36E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene06821 | 0.859181 | 4.94E-63 | -1.69E-46 |
| MS.gene052340 | MS.gene06821 | 0.80292 | 4.46E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene06821 | 0.817209 | 3.71E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene06821 | 0.895118 | 1.30E-75 | -1.69E-46 |
| MS.gene053907 | MS.gene06821 | 0.808499 | 3.01E-50 | -1.69E-46 |
| MS.gene053971 | MS.gene06821 | 0.80093 | 1.14E-48 | -1.69E-46 |
| MS.gene054010 | MS.gene06821 | 0.801759 | 7.74E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene06821 | 0.82398 | 1.03E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene06821 | 0.817512 | 3.17E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene06821 | 0.810405 | 1.17E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene06821 | 0.83851 | 2.75E-57 | -1.69E-46 |
| MS.gene054183 | MS.gene06821 | 0.822463 | 2.34E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene06821 | 0.864314 | 1.33E-64 | -1.69E-46 |
| MS.gene05455 | MS.gene06821 | 0.835798 | 1.36E-56 | -1.69E-46 |
| MS.gene054802 | MS.gene06821 | 0.805104 | 1.57E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene06821 | 0.801246 | 9.86E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene06821 | 0.803675 | 3.11E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene06821 | 0.807788 | 4.26E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene06821 | 0.820307 | 7.35E-53 | -1.69E-46 |
| MS.gene054983 | MS.gene06821 | 0.827872 | 1.23E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene06821 | 0.826714 | 2.33E-54 | -1.69E-46 |
| MS.gene055277 | MS.gene06821 | 0.877657 | 5.27E-69 | -1.69E-46 |
| MS.gene055303 | MS.gene06821 | 0.815674 | 8.19E-52 | -1.69E-46 |
| MS.gene055390 | MS.gene06821 | 0.85406 | 1.58E-61 | -1.69E-46 |
| MS.gene055422 | MS.gene06821 | 0.892026 | 2.32E-74 | -1.69E-46 |
| MS.gene055428 | MS.gene06821 | 0.89243 | 1.60E-74 | -1.69E-46 |
| MS.gene055604 | MS.gene06821 | 0.833303 | 5.76E-56 | -1.69E-46 |
| MS.gene055712 | MS.gene06821 | 0.829315 | 5.51E-55 | -1.69E-46 |
| MS.gene056191 | MS.gene06821 | 0.847539 | 1.08E-59 | -1.69E-46 |
| MS.gene056407 | MS.gene06821 | 0.844426 | 7.62E-59 | -1.69E-46 |
| MS.gene056451 | MS.gene06821 | 0.804135 | 2.50E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene06821 | 0.843913 | 1.05E-58 | -1.69E-46 |
| MS.gene056723 | MS.gene06821 | 0.808155 | 3.56E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene06821 | -0.801028 | 1.09E-48 | -1.69E-46 |
| MS.gene056902 | MS.gene06821 | 0.800495 | 1.40E-48 | -1.69E-46 |
| MS.gene05702 | MS.gene06821 | 0.845142 | 4.88E-59 | -1.69E-46 |
| MS.gene057121 | MS.gene06821 | 0.872114 | 4.08E-67 | -1.69E-46 |
| MS.gene057245 | MS.gene06821 | 0.932694 | 5.79E-95 | -1.69E-46 |
| MS.gene05733 | MS.gene06821 | 0.823514 | 1.33E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene06821 | 0.835593 | 1.53E-56 | -1.69E-46 |
| MS.gene057469 | MS.gene06821 | 0.81897 | 1.48E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene06821 | 0.818675 | 1.73E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene06821 | 0.809483 | 1.85E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene06821 | 0.870467 | 1.43E-66 | -1.69E-46 |
| MS.gene05811 | MS.gene06821 | 0.858145 | 1.01E-62 | -1.69E-46 |
| MS.gene058142 | MS.gene06821 | 0.819766 | 9.77E-53 | -1.69E-46 |
| MS.gene058233 | MS.gene06821 | 0.826518 | 2.60E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene06821 | 0.825379 | 4.84E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene06821 | 0.880609 | 4.77E-70 | -1.69E-46 |
| MS.gene058749 | MS.gene06821 | 0.80578 | 1.13E-49 | -1.69E-46 |
| MS.gene05886 | MS.gene06821 | 0.844968 | 5.44E-59 | -1.69E-46 |
| MS.gene05907 | MS.gene06821 | 0.861693 | 8.58E-64 | -1.69E-46 |
| MS.gene059420 | MS.gene06821 | 0.833111 | 6.43E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene06821 | 0.836516 | 8.94E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene06821 | 0.841762 | 3.90E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene06821 | 0.815828 | 7.57E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene06821 | 0.823887 | 1.09E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene06821 | 0.824306 | 8.67E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene06821 | 0.82195 | 3.07E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene06821 | 0.860021 | 2.76E-63 | -1.69E-46 |
| MS.gene059894 | MS.gene06821 | 0.834069 | 3.71E-56 | -1.69E-46 |
| MS.gene06037 | MS.gene06821 | 0.85014 | 2.06E-60 | -1.69E-46 |
| MS.gene060559 | MS.gene06821 | 0.815547 | 8.74E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene06821 | 0.863303 | 2.74E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06821.t1 | MTR_3g092710 | 85.573 | 506 | 19 | 2 | 1 | 452 | 1 | 506 | 0.0 | 842 |
| MS.gene06821.t1 | MTR_2g034600 | 25.321 | 312 | 182 | 11 | 133 | 437 | 280 | 547 | 1.28e-20 | 95.1 |
| MS.gene06821.t1 | MTR_2g034600 | 25.321 | 312 | 182 | 11 | 133 | 437 | 276 | 543 | 1.30e-20 | 94.7 |
| MS.gene06821.t1 | MTR_2g034600 | 25.321 | 312 | 182 | 11 | 133 | 437 | 215 | 482 | 1.36e-20 | 94.7 |
| MS.gene06821.t1 | MTR_4g084630 | 25.000 | 264 | 162 | 5 | 175 | 437 | 90 | 318 | 3.05e-14 | 73.9 |
| MS.gene06821.t1 | MTR_4g070060 | 22.055 | 399 | 255 | 11 | 29 | 415 | 210 | 564 | 3.15e-14 | 75.5 |
| MS.gene06821.t1 | MTR_4g120380 | 22.183 | 284 | 202 | 8 | 165 | 437 | 75 | 350 | 5.13e-13 | 70.5 |
| MS.gene06821.t1 | MTR_4g120380 | 22.184 | 293 | 209 | 8 | 156 | 437 | 66 | 350 | 6.21e-13 | 70.9 |
| MS.gene06821.t1 | MTR_1g115415 | 23.151 | 311 | 192 | 8 | 181 | 450 | 158 | 462 | 1.82e-12 | 69.7 |
| MS.gene06821.t1 | MTR_6g003960 | 22.256 | 328 | 210 | 13 | 135 | 439 | 284 | 589 | 1.03e-11 | 67.4 |
| MS.gene06821.t1 | MTR_2g437030 | 24.324 | 296 | 193 | 9 | 159 | 437 | 153 | 434 | 1.59e-11 | 66.6 |
| MS.gene06821.t1 | MTR_5g068860 | 23.333 | 420 | 267 | 16 | 28 | 437 | 108 | 482 | 2.46e-11 | 66.2 |
| MS.gene06821.t1 | MTR_8g005155 | 29.412 | 136 | 89 | 2 | 310 | 440 | 407 | 540 | 3.59e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06821.t1 | AT5G54180 | 53.771 | 411 | 189 | 1 | 28 | 438 | 80 | 489 | 3.46e-150 | 437 |
| MS.gene06821.t1 | AT4G14605 | 28.175 | 252 | 161 | 11 | 194 | 437 | 228 | 467 | 8.24e-17 | 83.2 |
| MS.gene06821.t1 | AT4G14605 | 28.175 | 252 | 161 | 11 | 194 | 437 | 228 | 467 | 8.24e-17 | 83.2 |
| MS.gene06821.t1 | AT2G36000 | 29.545 | 220 | 135 | 8 | 214 | 420 | 81 | 293 | 6.03e-15 | 76.3 |
| MS.gene06821.t1 | AT4G38160 | 26.538 | 260 | 173 | 8 | 172 | 417 | 46 | 301 | 6.67e-15 | 75.9 |
| MS.gene06821.t1 | AT4G38160 | 26.538 | 260 | 173 | 8 | 172 | 417 | 46 | 301 | 6.67e-15 | 75.9 |
| MS.gene06821.t1 | AT4G38160 | 26.538 | 260 | 173 | 8 | 172 | 417 | 46 | 301 | 8.13e-15 | 76.3 |
| MS.gene06821.t1 | AT4G38160 | 26.538 | 260 | 173 | 8 | 172 | 417 | 46 | 301 | 9.75e-15 | 75.9 |
| MS.gene06821.t1 | AT2G36000 | 29.545 | 220 | 135 | 8 | 214 | 420 | 81 | 293 | 1.23e-14 | 75.1 |
| MS.gene06821.t1 | AT2G03050 | 27.273 | 198 | 132 | 5 | 247 | 437 | 76 | 268 | 6.55e-13 | 69.3 |
| MS.gene06821.t1 | AT2G03050 | 27.273 | 198 | 132 | 5 | 247 | 437 | 77 | 269 | 7.14e-13 | 69.3 |
| MS.gene06821.t1 | AT5G07900 | 26.054 | 261 | 173 | 8 | 182 | 430 | 121 | 373 | 2.74e-12 | 68.6 |
| MS.gene06821.t1 | AT2G21710 | 22.517 | 302 | 218 | 7 | 124 | 415 | 276 | 571 | 2.23e-11 | 66.6 |
| MS.gene06821.t1 | AT2G44020 | 22.772 | 404 | 213 | 13 | 127 | 437 | 58 | 455 | 2.42e-11 | 66.2 |
| MS.gene06821.t1 | AT1G78930 | 23.952 | 334 | 233 | 11 | 116 | 439 | 254 | 576 | 3.06e-11 | 65.9 |
Find 74 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGAGCCTATTCTTGAACTA+TGG | 0.173687 | 3.1:+74761580 | MS.gene06821:CDS |
| TAAGATTGTGTTGGTGTTTC+CGG | 0.175674 | 3.1:+74761306 | MS.gene06821:CDS |
| ATGAAATAAAGAAGGGTTTA+AGG | 0.183717 | 3.1:+74761785 | MS.gene06821:CDS |
| TCCTTGAGAAATTGTATTCT+AGG | 0.235507 | 3.1:-74761364 | None:intergenic |
| AACAGTTAAAAGCTTATTGA+TGG | 0.295956 | 3.1:-74761821 | None:intergenic |
| TGAAACGTTGTTATAGAAAA+AGG | 0.303498 | 3.1:-74760338 | None:intergenic |
| TCAGTAAGAACTCTAATTCT+AGG | 0.304339 | 3.1:-74761145 | None:intergenic |
| GTCTCTTTATTTGAAGAATT+CGG | 0.325435 | 3.1:+74760635 | MS.gene06821:CDS |
| ACACTATAATTCAAGATAAT+AGG | 0.335022 | 3.1:-74761217 | None:intergenic |
| TAAAATCTGAATTGAAATAA+AGG | 0.346570 | 3.1:+74761887 | MS.gene06821:CDS |
| GTATTGGGAAAGTGCTGAAT+AGG | 0.369869 | 3.1:+74761191 | MS.gene06821:CDS |
| TCGTGACATTCAAGAGTTAC+TGG | 0.371309 | 3.1:+74761711 | MS.gene06821:CDS |
| ATAGATTTCTTAAAACCCTT+TGG | 0.390276 | 3.1:+74761058 | MS.gene06821:CDS |
| TCAAGATTGGGTATAAATAT+AGG | 0.394877 | 3.1:+74761491 | MS.gene06821:CDS |
| GTTCGATTGCTTGTTGATAG+TGG | 0.398687 | 3.1:+74760944 | MS.gene06821:CDS |
| ATGTGGATAATTTGATGGAT+AGG | 0.399161 | 3.1:-74760363 | None:intergenic |
| ATGGAGATGAGGATAGTATT+GGG | 0.408002 | 3.1:+74761176 | MS.gene06821:CDS |
| CGAACCTTGCGTATAAGCTT+GGG | 0.418895 | 3.1:+74761461 | MS.gene06821:CDS |
| TTCAGTGACTAAGTGATTGA+TGG | 0.422092 | 3.1:-74760777 | None:intergenic |
| GCGAACCTTGCGTATAAGCT+TGG | 0.427203 | 3.1:+74761460 | MS.gene06821:CDS |
| CGGGAATGTGGATAATTTGA+TGG | 0.434109 | 3.1:-74760368 | None:intergenic |
| GATGGAGATGAGGATAGTAT+TGG | 0.437973 | 3.1:+74761175 | MS.gene06821:CDS |
| TGAACTTAACTCTATTCCAT+TGG | 0.447457 | 3.1:+74760700 | MS.gene06821:CDS |
| TAAATATTTGTTGATCATCA+AGG | 0.451246 | 3.1:-74761285 | None:intergenic |
| AAAACCCATAGTTCAAGAAT+AGG | 0.452781 | 3.1:-74761585 | None:intergenic |
| ACATGTACAATCTTATCAAC+AGG | 0.460797 | 3.1:-74760971 | None:intergenic |
| TGAAATAAAGAAGGGTTTAA+GGG | 0.465888 | 3.1:+74761786 | MS.gene06821:CDS |
| TGTCGAAGGCGATTTGTCAT+AGG | 0.466844 | 3.1:+74761011 | MS.gene06821:CDS |
| CAGTTTCCTCTCTCTGCTAC+TGG | 0.469436 | 3.1:-74761338 | None:intergenic |
| GAAGATGGAGTACTTGATAG+AGG | 0.472033 | 3.1:+74761681 | MS.gene06821:CDS |
| TGAGCCTATTCTTGAACTAT+GGG | 0.472998 | 3.1:+74761581 | MS.gene06821:CDS |
| TTTATCTGGGTTTCCTCAGA+AGG | 0.474030 | 3.1:+74760922 | MS.gene06821:CDS |
| GATTTCTTAAAACCCTTTGG+TGG | 0.492456 | 3.1:+74761061 | MS.gene06821:CDS |
| GCATAGGTATGAAATAAAGA+AGG | 0.498746 | 3.1:+74761777 | MS.gene06821:CDS |
| ACAAATATTTAAGATTGTGT+TGG | 0.499280 | 3.1:+74761297 | MS.gene06821:CDS |
| GTTGAGGGTGAACGGGAATG+TGG | 0.504353 | 3.1:-74760380 | None:intergenic |
| AAATCACAATTGAGAATCAC+AGG | 0.505679 | 3.1:-74761106 | None:intergenic |
| TACAATTTCTCAAGGAATGT+GGG | 0.508650 | 3.1:+74761371 | MS.gene06821:CDS |
| CATAGGTATGAAATAAAGAA+GGG | 0.511589 | 3.1:+74761778 | MS.gene06821:CDS |
| TGGGTATAAATATAGGACTA+AGG | 0.519559 | 3.1:+74761498 | MS.gene06821:CDS |
| AATGGAATAGAGTTAAGTTC+AGG | 0.520347 | 3.1:-74760698 | None:intergenic |
| TGTGGATAATTTGATGGATA+GGG | 0.522628 | 3.1:-74760362 | None:intergenic |
| GTTCTTACTGAGTTAAGTGA+TGG | 0.523874 | 3.1:+74761157 | MS.gene06821:CDS |
| AGCTTGTATCCAAGAAATGC+AGG | 0.531555 | 3.1:-74761739 | None:intergenic |
| GTTTCAAGTGAGAACTTCAC+TGG | 0.532193 | 3.1:+74761841 | MS.gene06821:CDS |
| CTGCTACTGGTAAAGATAGC+CGG | 0.532788 | 3.1:-74761325 | None:intergenic |
| GAAATAAAGAAGGGTTTAAG+GGG | 0.547648 | 3.1:+74761787 | MS.gene06821:CDS |
| ATACAATTTCTCAAGGAATG+TGG | 0.550137 | 3.1:+74761370 | MS.gene06821:CDS |
| ATCAAATGAATACCACCAAA+GGG | 0.550277 | 3.1:-74761073 | None:intergenic |
| ATGGAATAGAGTTAAGTTCA+GGG | 0.553719 | 3.1:-74760697 | None:intergenic |
| GCTTATACGCAAGGTTCGCA+CGG | 0.556457 | 3.1:-74761456 | None:intergenic |
| CGATCACGCAATGAATCCAA+TGG | 0.559201 | 3.1:-74760716 | None:intergenic |
| TAACAAAGTAAATTTGTCGA+AGG | 0.566339 | 3.1:+74760997 | MS.gene06821:CDS |
| TATTATCTTGAATTATAGTG+TGG | 0.569717 | 3.1:+74761219 | MS.gene06821:CDS |
| AATCAAATGAATACCACCAA+AGG | 0.571779 | 3.1:-74761074 | None:intergenic |
| TTATAGTGTGGAACATCTTG+AGG | 0.578549 | 3.1:+74761231 | MS.gene06821:CDS |
| ACAAGCAATCGAACCTTCTG+AGG | 0.581418 | 3.1:-74760935 | None:intergenic |
| CTCCATCTTCTTCTCTAGAG+AGG | 0.583295 | 3.1:-74761668 | None:intergenic |
| TGATTATACTGCAGTATCTG+AGG | 0.588367 | 3.1:-74761643 | None:intergenic |
| TCCTAGAATACAATTTCTCA+AGG | 0.589047 | 3.1:+74761363 | MS.gene06821:CDS |
| TTGAGAAATCGAAGCTGAAG+CGG | 0.589081 | 3.1:+74760873 | MS.gene06821:CDS |
| TGAGTTAAGTGATGGAGATG+AGG | 0.592800 | 3.1:+74761165 | MS.gene06821:CDS |
| AGGAACATATAAAGTTTCTG+AGG | 0.600236 | 3.1:+74761251 | MS.gene06821:CDS |
| CAAGAACTTGAAGATTTCGT+CGG | 0.602711 | 3.1:-74761401 | None:intergenic |
| CTGTGAGAATATGCAGAAGA+TGG | 0.604021 | 3.1:+74761558 | MS.gene06821:CDS |
| TGCCTCTCTAGAGAAGAAGA+TGG | 0.604406 | 3.1:+74761666 | MS.gene06821:CDS |
| AAAACCCAAGCTTATACGCA+AGG | 0.605961 | 3.1:-74761465 | None:intergenic |
| TTGATGACAGAATTAAGCAT+AGG | 0.632416 | 3.1:+74761761 | MS.gene06821:CDS |
| TCTTTACCAGTAGCAGAGAG+AGG | 0.644660 | 3.1:+74761332 | MS.gene06821:CDS |
| AAATAAAGAAGGGTTTAAGG+GGG | 0.657424 | 3.1:+74761788 | MS.gene06821:CDS |
| TGGAATAGAGTTAAGTTCAG+GGG | 0.673962 | 3.1:-74760696 | None:intergenic |
| AATAAAGAAGGGTTTAAGGG+GGG | 0.680018 | 3.1:+74761789 | MS.gene06821:CDS |
| TAGGACTAAGGAGTTAGCAG+TGG | 0.681301 | 3.1:+74761510 | MS.gene06821:CDS |
| AATCACAATTGAGAATCACA+GGG | 0.722130 | 3.1:-74761105 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACACTATAATTCAAGATAAT+AGG | - | chr3.1:74761220-74761239 | None:intergenic | 20.0% |
| !! | TATTATCTTGAATTATAGTG+TGG | + | chr3.1:74761219-74761238 | MS.gene06821:CDS | 20.0% |
| !!! | ACAAATATTTAAGATTGTGT+TGG | + | chr3.1:74761297-74761316 | MS.gene06821:CDS | 20.0% |
| !!! | ATTTTAATTTTTCATGTTGC+AGG | + | chr3.1:74760605-74760624 | MS.gene06821:intron | 20.0% |
| !!! | TAAATATTTGTTGATCATCA+AGG | - | chr3.1:74761288-74761307 | None:intergenic | 20.0% |
| !!! | TTTTTGAGAAATGAATTACA+AGG | + | chr3.1:74760845-74760864 | MS.gene06821:CDS | 20.0% |
| ! | AACAGTTAAAAGCTTATTGA+TGG | - | chr3.1:74761824-74761843 | None:intergenic | 25.0% |
| ! | AATGAACCGAAAAATTTATC+TGG | + | chr3.1:74760908-74760927 | MS.gene06821:CDS | 25.0% |
| ! | ATAGATTTCTTAAAACCCTT+TGG | + | chr3.1:74761058-74761077 | MS.gene06821:CDS | 25.0% |
| ! | ATGAAATAAAGAAGGGTTTA+AGG | + | chr3.1:74761785-74761804 | MS.gene06821:CDS | 25.0% |
| ! | ATGAACCGAAAAATTTATCT+GGG | + | chr3.1:74760909-74760928 | MS.gene06821:CDS | 25.0% |
| ! | CATAGGTATGAAATAAAGAA+GGG | + | chr3.1:74761778-74761797 | MS.gene06821:CDS | 25.0% |
| ! | TAACAAAGTAAATTTGTCGA+AGG | + | chr3.1:74760997-74761016 | MS.gene06821:CDS | 25.0% |
| ! | TCAAGATTGGGTATAAATAT+AGG | + | chr3.1:74761491-74761510 | MS.gene06821:CDS | 25.0% |
| ! | TGAAACGTTGTTATAGAAAA+AGG | - | chr3.1:74760341-74760360 | None:intergenic | 25.0% |
| ! | TGAAATAAAGAAGGGTTTAA+GGG | + | chr3.1:74761786-74761805 | MS.gene06821:CDS | 25.0% |
| ! | TTTCTCAAAAAACTCAACAA+TGG | - | chr3.1:74760836-74760855 | None:intergenic | 25.0% |
| !! | GTCTCTTTATTTGAAGAATT+CGG | + | chr3.1:74760635-74760654 | MS.gene06821:CDS | 25.0% |
| !!! | GTTTTTTCATTATACTCTCT+CGG | + | chr3.1:74760740-74760759 | MS.gene06821:CDS | 25.0% |
| !!! | TTTTTTCATTATACTCTCTC+GGG | + | chr3.1:74760741-74760760 | MS.gene06821:CDS | 25.0% |
| AAAACCCATAGTTCAAGAAT+AGG | - | chr3.1:74761588-74761607 | None:intergenic | 30.0% | |
| AAATAAAGAAGGGTTTAAGG+GGG | + | chr3.1:74761788-74761807 | MS.gene06821:CDS | 30.0% | |
| AAATCACAATTGAGAATCAC+AGG | - | chr3.1:74761109-74761128 | None:intergenic | 30.0% | |
| AATCAAATGAATACCACCAA+AGG | - | chr3.1:74761077-74761096 | None:intergenic | 30.0% | |
| AATCACAATTGAGAATCACA+GGG | - | chr3.1:74761108-74761127 | None:intergenic | 30.0% | |
| AATGGAATAGAGTTAAGTTC+AGG | - | chr3.1:74760701-74760720 | None:intergenic | 30.0% | |
| ACATGTACAATCTTATCAAC+AGG | - | chr3.1:74760974-74760993 | None:intergenic | 30.0% | |
| AGGAACATATAAAGTTTCTG+AGG | + | chr3.1:74761251-74761270 | MS.gene06821:CDS | 30.0% | |
| ATACAATTTCTCAAGGAATG+TGG | + | chr3.1:74761370-74761389 | MS.gene06821:CDS | 30.0% | |
| ATCAAATGAATACCACCAAA+GGG | - | chr3.1:74761076-74761095 | None:intergenic | 30.0% | |
| ATGGAATAGAGTTAAGTTCA+GGG | - | chr3.1:74760700-74760719 | None:intergenic | 30.0% | |
| GAAAATGAAAACGGAAAAAG+TGG | - | chr3.1:74760472-74760491 | None:intergenic | 30.0% | |
| GAAATAAAGAAGGGTTTAAG+GGG | + | chr3.1:74761787-74761806 | MS.gene06821:CDS | 30.0% | |
| GCATAGGTATGAAATAAAGA+AGG | + | chr3.1:74761777-74761796 | MS.gene06821:CDS | 30.0% | |
| TACAATTTCTCAAGGAATGT+GGG | + | chr3.1:74761371-74761390 | MS.gene06821:CDS | 30.0% | |
| TCAGTAAGAACTCTAATTCT+AGG | - | chr3.1:74761148-74761167 | None:intergenic | 30.0% | |
| TCCTAGAATACAATTTCTCA+AGG | + | chr3.1:74761363-74761382 | MS.gene06821:CDS | 30.0% | |
| TCCTTGAGAAATTGTATTCT+AGG | - | chr3.1:74761367-74761386 | None:intergenic | 30.0% | |
| TGAACTTAACTCTATTCCAT+TGG | + | chr3.1:74760700-74760719 | MS.gene06821:CDS | 30.0% | |
| TGGGTATAAATATAGGACTA+AGG | + | chr3.1:74761498-74761517 | MS.gene06821:CDS | 30.0% | |
| ! | ATGTGGATAATTTGATGGAT+AGG | - | chr3.1:74760366-74760385 | None:intergenic | 30.0% |
| ! | GGAAACCCAGATAAATTTTT+CGG | - | chr3.1:74760917-74760936 | None:intergenic | 30.0% |
| ! | TGTGGATAATTTGATGGATA+GGG | - | chr3.1:74760365-74760384 | None:intergenic | 30.0% |
| ! | TTGATGACAGAATTAAGCAT+AGG | + | chr3.1:74761761-74761780 | MS.gene06821:CDS | 30.0% |
| ! | TTGGGTTTTTGATCAAGATT+GGG | + | chr3.1:74761479-74761498 | MS.gene06821:CDS | 30.0% |
| AAACGGAAAAAGTGGTTGTT+TGG | - | chr3.1:74760464-74760483 | None:intergenic | 35.0% | |
| AATAAAGAAGGGTTTAAGGG+GGG | + | chr3.1:74761789-74761808 | MS.gene06821:CDS | 35.0% | |
| ATGGAGATGAGGATAGTATT+GGG | + | chr3.1:74761176-74761195 | MS.gene06821:CDS | 35.0% | |
| CAAGAACTTGAAGATTTCGT+CGG | - | chr3.1:74761404-74761423 | None:intergenic | 35.0% | |
| GATTTCTTAAAACCCTTTGG+TGG | + | chr3.1:74761061-74761080 | MS.gene06821:CDS | 35.0% | |
| GTTCTTACTGAGTTAAGTGA+TGG | + | chr3.1:74761157-74761176 | MS.gene06821:CDS | 35.0% | |
| TACATTAGCGAATGCATGTA+AGG | - | chr3.1:74760586-74760605 | None:intergenic | 35.0% | |
| TGAGCCTATTCTTGAACTAT+GGG | + | chr3.1:74761581-74761600 | MS.gene06821:CDS | 35.0% | |
| TGATTATACTGCAGTATCTG+AGG | - | chr3.1:74761646-74761665 | None:intergenic | 35.0% | |
| TGGAATAGAGTTAAGTTCAG+GGG | - | chr3.1:74760699-74760718 | None:intergenic | 35.0% | |
| TTATAGTGTGGAACATCTTG+AGG | + | chr3.1:74761231-74761250 | MS.gene06821:CDS | 35.0% | |
| TTCAGTGACTAAGTGATTGA+TGG | - | chr3.1:74760780-74760799 | None:intergenic | 35.0% | |
| ! | CTTGGGTTTTTGATCAAGAT+TGG | + | chr3.1:74761478-74761497 | MS.gene06821:CDS | 35.0% |
| ! | TAAGATTGTGTTGGTGTTTC+CGG | + | chr3.1:74761306-74761325 | MS.gene06821:CDS | 35.0% |
| AAAACCCAAGCTTATACGCA+AGG | - | chr3.1:74761468-74761487 | None:intergenic | 40.0% | |
| ACCGAATGACACATTGACAT+TGG | - | chr3.1:74760531-74760550 | None:intergenic | 40.0% | |
| AGCTTGTATCCAAGAAATGC+AGG | - | chr3.1:74761742-74761761 | None:intergenic | 40.0% | |
| AGGGTTGAGTTTGAAGATGA+GGG | - | chr3.1:74760412-74760431 | None:intergenic | 40.0% | |
| AGTTTGAAGATGAGGGGAAA+GGG | - | chr3.1:74760405-74760424 | None:intergenic | 40.0% | |
| CCGAATGACACATTGACATT+GGG | - | chr3.1:74760530-74760549 | None:intergenic | 40.0% | |
| CGAATGACACATTGACATTG+GGG | - | chr3.1:74760529-74760548 | None:intergenic | 40.0% | |
| CGGGAATGTGGATAATTTGA+TGG | - | chr3.1:74760371-74760390 | None:intergenic | 40.0% | |
| CTGTGAGAATATGCAGAAGA+TGG | + | chr3.1:74761558-74761577 | MS.gene06821:CDS | 40.0% | |
| GAATGACACATTGACATTGG+GGG | - | chr3.1:74760528-74760547 | None:intergenic | 40.0% | |
| GATGGAGATGAGGATAGTAT+TGG | + | chr3.1:74761175-74761194 | MS.gene06821:CDS | 40.0% | |
| GTGAGCCTATTCTTGAACTA+TGG | + | chr3.1:74761580-74761599 | MS.gene06821:CDS | 40.0% | |
| TCGTGACATTCAAGAGTTAC+TGG | + | chr3.1:74761711-74761730 | MS.gene06821:CDS | 40.0% | |
| TGGAGGGTCGAAAATGAAAA+CGG | - | chr3.1:74760481-74760500 | None:intergenic | 40.0% | |
| TTGAGAAATCGAAGCTGAAG+CGG | + | chr3.1:74760873-74760892 | MS.gene06821:CDS | 40.0% | |
| TTTATCTGGGTTTCCTCAGA+AGG | + | chr3.1:74760922-74760941 | MS.gene06821:CDS | 40.0% | |
| ! | AAAAAGTGGTTGTTTGGTGG+TGG | - | chr3.1:74760458-74760477 | None:intergenic | 40.0% |
| ! | CCCAATGTCAATGTGTCATT+CGG | + | chr3.1:74760527-74760546 | MS.gene06821:intron | 40.0% |
| ! | CTGGATTTTCCTGCATTTCT+TGG | + | chr3.1:74761730-74761749 | MS.gene06821:CDS | 40.0% |
| ! | GAAGATGGAGTACTTGATAG+AGG | + | chr3.1:74761681-74761700 | MS.gene06821:CDS | 40.0% |
| ! | GTTCGATTGCTTGTTGATAG+TGG | + | chr3.1:74760944-74760963 | MS.gene06821:CDS | 40.0% |
| ! | GTTTCAAGTGAGAACTTCAC+TGG | + | chr3.1:74761841-74761860 | MS.gene06821:CDS | 40.0% |
| ! | TGAGTTAAGTGATGGAGATG+AGG | + | chr3.1:74761165-74761184 | MS.gene06821:CDS | 40.0% |
| !! | GTATTGGGAAAGTGCTGAAT+AGG | + | chr3.1:74761191-74761210 | MS.gene06821:CDS | 40.0% |
| ACAAGCAATCGAACCTTCTG+AGG | - | chr3.1:74760938-74760957 | None:intergenic | 45.0% | |
| AGTAACTACTACAGCGGCAA+CGG | - | chr3.1:74760505-74760524 | None:intergenic | 45.0% | |
| CGAACCTTGCGTATAAGCTT+GGG | + | chr3.1:74761461-74761480 | MS.gene06821:CDS | 45.0% | |
| CGATCACGCAATGAATCCAA+TGG | - | chr3.1:74760719-74760738 | None:intergenic | 45.0% | |
| CGGAAAAAGTGGTTGTTTGG+TGG | - | chr3.1:74760461-74760480 | None:intergenic | 45.0% | |
| CTGCTACTGGTAAAGATAGC+CGG | - | chr3.1:74761328-74761347 | None:intergenic | 45.0% | |
| GAGGGTTGAGTTTGAAGATG+AGG | - | chr3.1:74760413-74760432 | None:intergenic | 45.0% | |
| GAGTTTGAAGATGAGGGGAA+AGG | - | chr3.1:74760406-74760425 | None:intergenic | 45.0% | |
| GCTCAAAAACAACGCTGCTT+TGG | - | chr3.1:74761428-74761447 | None:intergenic | 45.0% | |
| GGGTTGAGTTTGAAGATGAG+GGG | - | chr3.1:74760411-74760430 | None:intergenic | 45.0% | |
| TAGGACTAAGGAGTTAGCAG+TGG | + | chr3.1:74761510-74761529 | MS.gene06821:CDS | 45.0% | |
| TCTTTACCAGTAGCAGAGAG+AGG | + | chr3.1:74761332-74761351 | MS.gene06821:CDS | 45.0% | |
| TGTCGAAGGCGATTTGTCAT+AGG | + | chr3.1:74761011-74761030 | MS.gene06821:CDS | 45.0% | |
| ! | CTCCATCTTCTTCTCTAGAG+AGG | - | chr3.1:74761671-74761690 | None:intergenic | 45.0% |
| ! | TGATTTGGCGAGGAAGGAAA+AGG | - | chr3.1:74760435-74760454 | None:intergenic | 45.0% |
| ! | TGCCTCTCTAGAGAAGAAGA+TGG | + | chr3.1:74761666-74761685 | MS.gene06821:CDS | 45.0% |
| !! | GTTGTTTGGTGGTGGTGATT+TGG | - | chr3.1:74760450-74760469 | None:intergenic | 45.0% |
| AAGATGAGGGGAAAGGGTTG+AGG | - | chr3.1:74760399-74760418 | None:intergenic | 50.0% | |
| AGATGAGGGGAAAGGGTTGA+GGG | - | chr3.1:74760398-74760417 | None:intergenic | 50.0% | |
| CAGTTTCCTCTCTCTGCTAC+TGG | - | chr3.1:74761341-74761360 | None:intergenic | 50.0% | |
| GCGAACCTTGCGTATAAGCT+TGG | + | chr3.1:74761460-74761479 | MS.gene06821:CDS | 50.0% | |
| GCTTATACGCAAGGTTCGCA+CGG | - | chr3.1:74761459-74761478 | None:intergenic | 50.0% | |
| TGGGGGAGTAACTACTACAG+CGG | - | chr3.1:74760511-74760530 | None:intergenic | 50.0% | |
| TTGGCGAGGAAGGAAAAGGA+GGG | - | chr3.1:74760431-74760450 | None:intergenic | 50.0% | |
| TTTGGCGAGGAAGGAAAAGG+AGG | - | chr3.1:74760432-74760451 | None:intergenic | 50.0% | |
| ACTACTACAGCGGCAACGGA+TGG | - | chr3.1:74760501-74760520 | None:intergenic | 55.0% | |
| GGAAAGGGTTGAGGGTGAAC+GGG | - | chr3.1:74760390-74760409 | None:intergenic | 55.0% | |
| GGGAAAGGGTTGAGGGTGAA+CGG | - | chr3.1:74760391-74760410 | None:intergenic | 55.0% | |
| GTTGAGGGTGAACGGGAATG+TGG | - | chr3.1:74760383-74760402 | None:intergenic | 55.0% | |
| ! | TGGTGGTGATTTGGCGAGGA+AGG | - | chr3.1:74760441-74760460 | None:intergenic | 55.0% |
| !! | TTGGTGGTGGTGATTTGGCG+AGG | - | chr3.1:74760445-74760464 | None:intergenic | 55.0% |
| ACTACAGCGGCAACGGATGG+AGG | - | chr3.1:74760498-74760517 | None:intergenic | 60.0% | |
| CTACAGCGGCAACGGATGGA+GGG | - | chr3.1:74760497-74760516 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 74760311 | 74761906 | 74760311 | ID=MS.gene06821 |
| chr3.1 | mRNA | 74760311 | 74761906 | 74760311 | ID=MS.gene06821.t1;Parent=MS.gene06821 |
| chr3.1 | exon | 74760311 | 74760389 | 74760311 | ID=MS.gene06821.t1.exon1;Parent=MS.gene06821.t1 |
| chr3.1 | CDS | 74760311 | 74760389 | 74760311 | ID=cds.MS.gene06821.t1;Parent=MS.gene06821.t1 |
| chr3.1 | exon | 74760627 | 74761906 | 74760627 | ID=MS.gene06821.t1.exon2;Parent=MS.gene06821.t1 |
| chr3.1 | CDS | 74760627 | 74761906 | 74760627 | ID=cds.MS.gene06821.t1;Parent=MS.gene06821.t1 |
| Gene Sequence |
| Protein sequence |
