AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073696


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073696.t1 MTR_1g051205 97.156 211 6 0 1 211 188 398 7.20e-156 437
MS.gene073696.t1 MTR_0004s0610 96.682 211 7 0 1 211 189 399 7.15e-154 432
MS.gene073696.t1 MTR_5g068170 82.381 210 20 1 1 210 118 310 1.38e-115 332
MS.gene073696.t1 MTR_6g034745 78.723 47 10 0 108 154 271 317 2.43e-19 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073696.t1 AT3G42630 67.773 211 67 1 1 211 206 415 5.34e-105 308
MS.gene073696.t1 AT4G14190 30.332 211 133 5 1 201 291 497 7.75e-14 70.1

Find 47 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTACAACATTTAATATT+AGG 0.132936 1.1:+36082914 MS.gene073696:CDS
ATAGGAGGAACCAGATATTT+TGG 0.193003 1.1:+36083262 MS.gene073696:CDS
TAGAAGGCTTGGAAGGAATT+TGG 0.229388 1.1:+36083059 MS.gene073696:CDS
GTTCCTGATTTGGTTACTTA+TGG 0.229795 1.1:+36083012 MS.gene073696:CDS
GGTGAGTTTGTTAGAGATAT+TGG 0.235251 1.1:+36082766 MS.gene073696:CDS
TTTCGAGGATGGCGCTGTTT+TGG 0.262354 1.1:+36082947 MS.gene073696:CDS
GAAGAGAAAATTCTATGAAT+TGG 0.262799 1.1:+36082744 MS.gene073696:CDS
TGTTACAACATTTAATATTA+GGG 0.275442 1.1:+36082915 MS.gene073696:CDS
GCTTGCAGAGAGAGTTTGTT+AGG 0.289369 1.1:+36082866 MS.gene073696:CDS
GTTAGTAGTTCCAAAATATC+TGG 0.291120 1.1:-36083272 None:intergenic
GTGATTAGGGCTATGGCTTA+TGG 0.328397 1.1:+36082712 MS.gene073696:CDS
AATGAAATGGAGAATGCTTA+TGG 0.332682 1.1:+36082652 None:intergenic
GATTGAGGAAAATGTGATTA+GGG 0.333668 1.1:+36082699 MS.gene073696:CDS
TGAAAAGGTTGTTCCTGATT+TGG 0.336453 1.1:+36083002 MS.gene073696:CDS
AACAACAGAAATGGAGTTAT+AGG 0.338389 1.1:+36083208 MS.gene073696:CDS
TACAGATCCGTTTGTGTTTG+AGG 0.346471 1.1:+36083128 MS.gene073696:CDS
TTTGTTAGGATGGTTGAATC+AGG 0.352533 1.1:+36082880 MS.gene073696:CDS
TGATTGAGGAAAATGTGATT+AGG 0.365093 1.1:+36082698 MS.gene073696:CDS
ATGGAAGGTTTAAGAGATCT+AGG 0.371453 1.1:+36082671 MS.gene073696:CDS
AATATCTTAAGAAACATTAT+AGG 0.375645 1.1:+36083244 MS.gene073696:CDS
AAGAGAAAATTCTATGAATT+GGG 0.388525 1.1:+36082745 MS.gene073696:CDS
TGGATGCTTACTTGGATAGA+AGG 0.398737 1.1:+36083043 MS.gene073696:CDS
TTTGTTAGAGATATTGGTTT+AGG 0.403997 1.1:+36082772 MS.gene073696:CDS
GGTTTAGGTAGGAGAAATGT+TGG 0.413219 1.1:+36082787 MS.gene073696:CDS
CAACCATAAGTAACCAAATC+AGG 0.419881 1.1:-36083015 None:intergenic
ACAAGACACAACAACAGAAA+TGG 0.420204 1.1:+36083199 MS.gene073696:CDS
TTGTGTTGTGGATGCTTACT+TGG 0.434412 1.1:+36083035 MS.gene073696:CDS
AACATTTAATATTAGGGCTA+TGG 0.434657 1.1:+36082921 MS.gene073696:CDS
ACCTTAGTATTGAACATATG+AGG 0.439836 1.1:+36082977 MS.gene073696:CDS
AGGTGACTTTCATTTGAGTT+CGG 0.445273 1.1:+36083161 MS.gene073696:CDS
TCCTCATATGTTCAATACTA+AGG 0.456089 1.1:-36082978 None:intergenic
GGAAAATGTGATTAGGGCTA+TGG 0.480637 1.1:+36082705 MS.gene073696:CDS
GCTTACTTGGATAGAAGGCT+TGG 0.482566 1.1:+36083048 MS.gene073696:CDS
GGTTGAATCAGGGTTTCGTC+CGG 0.489825 1.1:+36082891 MS.gene073696:CDS
GCAGAGAGAGTTTGTTAGGA+TGG 0.492223 1.1:+36082870 MS.gene073696:CDS
TTAGAGATATTGGTTTAGGT+AGG 0.504799 1.1:+36082776 MS.gene073696:CDS
ATATTAAATGTTGTAACATC+CGG 0.509918 1.1:-36082910 None:intergenic
GTTTAGGTAGGAGAAATGTT+GGG 0.514544 1.1:+36082788 MS.gene073696:CDS
TTGTTAGGATGGTTGAATCA+GGG 0.518440 1.1:+36082881 MS.gene073696:CDS
TGAACATATGAGGAGTGAAA+AGG 0.526259 1.1:+36082987 MS.gene073696:CDS
CCTAAAACCTCAAACACAAA+CGG 0.528045 1.1:-36083135 None:intergenic
GGTTACTTATGGTTGTGTTG+TGG 0.542271 1.1:+36083023 MS.gene073696:CDS
ACTTGGATAGAAGGCTTGGA+AGG 0.547884 1.1:+36083052 MS.gene073696:CDS
AAATGGAGAATGCTTATGGA+AGG 0.557066 1.1:+36082656 None:intergenic
AACGGATCTGTAAACAATCT+CGG 0.566471 1.1:-36083117 None:intergenic
TGACTTTCATTTGAGTTCGG+AGG 0.582232 1.1:+36083164 MS.gene073696:CDS
ATCTTAAGAAACATTATAGG+AGG 0.601959 1.1:+36083247 MS.gene073696:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATCTTAAGAAACATTAT+AGG + chr1.1:36083244-36083263 MS.gene073696:CDS 15.0%
!! ATGTTACAACATTTAATATT+AGG + chr1.1:36082914-36082933 MS.gene073696:CDS 15.0%
!! TGTTACAACATTTAATATTA+GGG + chr1.1:36082915-36082934 MS.gene073696:CDS 15.0%
!! AAGAGAAAATTCTATGAATT+GGG + chr1.1:36082745-36082764 MS.gene073696:CDS 20.0%
!! ATATTAAATGTTGTAACATC+CGG - chr1.1:36082913-36082932 None:intergenic 20.0%
! AACATTTAATATTAGGGCTA+TGG + chr1.1:36082921-36082940 MS.gene073696:CDS 25.0%
! ATCTTAAGAAACATTATAGG+AGG + chr1.1:36083247-36083266 MS.gene073696:CDS 25.0%
! GAAGAGAAAATTCTATGAAT+TGG + chr1.1:36082744-36082763 MS.gene073696:CDS 25.0%
!!! GGAGTTTGTTTTGAATAAAA+TGG + chr1.1:36083080-36083099 MS.gene073696:CDS 25.0%
!!! TGTTTTGAATAAAATGGATG+TGG + chr1.1:36083086-36083105 MS.gene073696:CDS 25.0%
!!! TTTGTTAGAGATATTGGTTT+AGG + chr1.1:36082772-36082791 MS.gene073696:CDS 25.0%
AACAACAGAAATGGAGTTAT+AGG + chr1.1:36083208-36083227 MS.gene073696:CDS 30.0%
GATTGAGGAAAATGTGATTA+GGG + chr1.1:36082699-36082718 MS.gene073696:CDS 30.0%
TCCTCATATGTTCAATACTA+AGG - chr1.1:36082981-36083000 None:intergenic 30.0%
TGATTGAGGAAAATGTGATT+AGG + chr1.1:36082698-36082717 MS.gene073696:CDS 30.0%
! ACCTTAGTATTGAACATATG+AGG + chr1.1:36082977-36082996 MS.gene073696:CDS 30.0%
!! TTAGAGATATTGGTTTAGGT+AGG + chr1.1:36082776-36082795 MS.gene073696:CDS 30.0%
AACGGATCTGTAAACAATCT+CGG - chr1.1:36083120-36083139 None:intergenic 35.0%
ACAAGACACAACAACAGAAA+TGG + chr1.1:36083199-36083218 MS.gene073696:CDS 35.0%
ATGGAAGGTTTAAGAGATCT+AGG + chr1.1:36082671-36082690 MS.gene073696:CDS 35.0%
CAACCATAAGTAACCAAATC+AGG - chr1.1:36083018-36083037 None:intergenic 35.0%
CCTAAAACCTCAAACACAAA+CGG - chr1.1:36083138-36083157 None:intergenic 35.0%
GTTTAGGTAGGAGAAATGTT+GGG + chr1.1:36082788-36082807 MS.gene073696:CDS 35.0%
TGAAAAGGTTGTTCCTGATT+TGG + chr1.1:36083002-36083021 MS.gene073696:CDS 35.0%
TGAACATATGAGGAGTGAAA+AGG + chr1.1:36082987-36083006 MS.gene073696:CDS 35.0%
TTGTTAGGATGGTTGAATCA+GGG + chr1.1:36082881-36082900 MS.gene073696:CDS 35.0%
TTTGTTAGGATGGTTGAATC+AGG + chr1.1:36082880-36082899 MS.gene073696:CDS 35.0%
! AGGTGACTTTCATTTGAGTT+CGG + chr1.1:36083161-36083180 MS.gene073696:CDS 35.0%
! ATAGGAGGAACCAGATATTT+TGG + chr1.1:36083262-36083281 MS.gene073696:CDS 35.0%
! GAAATGTTGGGAATCTTTTG+TGG + chr1.1:36082800-36082819 MS.gene073696:CDS 35.0%
!! GAGATCTAGGTTTTTGATTG+AGG + chr1.1:36082684-36082703 MS.gene073696:CDS 35.0%
!! GGTGAGTTTGTTAGAGATAT+TGG + chr1.1:36082766-36082785 MS.gene073696:CDS 35.0%
!! GTTCCTGATTTGGTTACTTA+TGG + chr1.1:36083012-36083031 MS.gene073696:CDS 35.0%
!!! GTGTTTGAGGTTTTAGGTAA+AGG + chr1.1:36083141-36083160 MS.gene073696:CDS 35.0%
GGAAAATGTGATTAGGGCTA+TGG + chr1.1:36082705-36082724 MS.gene073696:CDS 40.0%
GGTTACTTATGGTTGTGTTG+TGG + chr1.1:36083023-36083042 MS.gene073696:CDS 40.0%
GGTTTAGGTAGGAGAAATGT+TGG + chr1.1:36082787-36082806 MS.gene073696:CDS 40.0%
TACAGATCCGTTTGTGTTTG+AGG + chr1.1:36083128-36083147 MS.gene073696:CDS 40.0%
TAGAAGGCTTGGAAGGAATT+TGG + chr1.1:36083059-36083078 MS.gene073696:CDS 40.0%
TGGATGCTTACTTGGATAGA+AGG + chr1.1:36083043-36083062 MS.gene073696:CDS 40.0%
TTGTGTTGTGGATGCTTACT+TGG + chr1.1:36083035-36083054 MS.gene073696:CDS 40.0%
! TGACTTTCATTTGAGTTCGG+AGG + chr1.1:36083164-36083183 MS.gene073696:CDS 40.0%
!! CCGTTTGTGTTTGAGGTTTT+AGG + chr1.1:36083135-36083154 MS.gene073696:CDS 40.0%
ACTTGGATAGAAGGCTTGGA+AGG + chr1.1:36083052-36083071 MS.gene073696:CDS 45.0%
GCTTACTTGGATAGAAGGCT+TGG + chr1.1:36083048-36083067 MS.gene073696:CDS 45.0%
GTGATTAGGGCTATGGCTTA+TGG + chr1.1:36082712-36082731 MS.gene073696:CDS 45.0%
! GCTTGCAGAGAGAGTTTGTT+AGG + chr1.1:36082866-36082885 MS.gene073696:CDS 45.0%
!! GCAGAGAGAGTTTGTTAGGA+TGG + chr1.1:36082870-36082889 MS.gene073696:CDS 45.0%
!!! TTTGAGTTCGGAGGCGTTTT+TGG + chr1.1:36083173-36083192 MS.gene073696:CDS 45.0%
GGTTGAATCAGGGTTTCGTC+CGG + chr1.1:36082891-36082910 MS.gene073696:CDS 50.0%
! TTAGGGCTATGGCGTTTTCG+AGG + chr1.1:36082932-36082951 MS.gene073696:CDS 50.0%
! TTTCGAGGATGGCGCTGTTT+TGG + chr1.1:36082947-36082966 MS.gene073696:CDS 50.0%
!! TTCGAGGATGGCGCTGTTTT+GGG + chr1.1:36082948-36082967 MS.gene073696:CDS 50.0%
!! GGCTATGGCGTTTTCGAGGA+TGG + chr1.1:36082936-36082955 MS.gene073696:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 36082658 36083293 36082658 ID=MS.gene073696
chr1.1 mRNA 36082658 36083293 36082658 ID=MS.gene073696.t1;Parent=MS.gene073696
chr1.1 exon 36082658 36083293 36082658 ID=MS.gene073696.t1.exon1;Parent=MS.gene073696.t1
chr1.1 CDS 36082658 36083293 36082658 ID=cds.MS.gene073696.t1;Parent=MS.gene073696.t1
Gene Sequence

>MS.gene073696

ATGGAGAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATTGAGGAAAATGTGATTAGGGCTATGGCTTATGGTTATACAAAGAAGAGAAAATTCTATGAATTGGGTGAGTTTGTTAGAGATATTGGTTTAGGTAGGAGAAATGTTGGGAATCTTTTGTGGAATCTTCTGTTGCTTTCTTATGCTGCTAATTTTAAGATGAAGAGCTTGCAGAGAGAGTTTGTTAGGATGGTTGAATCAGGGTTTCGTCCGGATGTTACAACATTTAATATTAGGGCTATGGCGTTTTCGAGGATGGCGCTGTTTTGGGATCTTCACCTTAGTATTGAACATATGAGGAGTGAAAAGGTTGTTCCTGATTTGGTTACTTATGGTTGTGTTGTGGATGCTTACTTGGATAGAAGGCTTGGAAGGAATTTGGAGTTTGTTTTGAATAAAATGGATGTGGATGATTGTCCGAGATTGTTTACAGATCCGTTTGTGTTTGAGGTTTTAGGTAAAGGTGACTTTCATTTGAGTTCGGAGGCGTTTTTGGAATACAAGACACAACAACAGAAATGGAGTTATAGGATTTTGATAAAGAAATATCTTAAGAAACATTATAGGAGGAACCAGATATTTTGGAACTACTAA

Protein sequence

>MS.gene073696.t1

MENAYGRFKRSRFLIEENVIRAMAYGYTKKRKFYELGEFVRDIGLGRRNVGNLLWNLLLLSYAANFKMKSLQREFVRMVESGFRPDVTTFNIRAMAFSRMALFWDLHLSIEHMRSEKVVPDLVTYGCVVDAYLDRRLGRNLEFVLNKMDVDDCPRLFTDPFVFEVLGKGDFHLSSEAFLEYKTQQQKWSYRILIKKYLKKHYRRNQIFWNY