AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073962


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073962.t1 MTR_4g116200 96.525 259 6 2 1 259 1 256 0.0 516
MS.gene073962.t1 MTR_2g450720 53.208 265 92 9 1 259 1 239 4.98e-83 248
MS.gene073962.t1 MTR_4g009760 48.667 150 68 4 1 142 1 149 2.95e-37 130
MS.gene073962.t1 MTR_2g104230 45.625 160 70 6 1 146 1 157 7.77e-33 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073962.t1 AT2G12462 33.929 280 133 13 1 259 1 249 9.64e-34 122
MS.gene073962.t1 AT1G15760 39.157 166 87 3 3 162 5 162 5.12e-31 114
MS.gene073962.t1 AT1G80520 51.923 104 43 2 1 98 1 103 4.48e-29 109

Find 48 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCAATGCTTTATTATTT+TGG 0.098715 8.3:+9891941 None:intergenic
TCACCTTTGGGTTTATTATA+AGG 0.198904 8.3:+9891709 None:intergenic
TCAATAGCCCTACTTCTTAA+TGG 0.233145 8.3:+9891856 None:intergenic
CCATCTAATGGTCCTGAATA+TGG 0.284057 8.3:+9891988 None:intergenic
ATCAAAATCAAAATCACCTT+TGG 0.295369 8.3:+9891696 None:intergenic
AATAAAGCATTGAAGTTATC+AGG 0.300318 8.3:-9891934 MS.gene073962:CDS
CATTCACTCATATATGATTA+TGG 0.310367 8.3:-9892363 MS.gene073962:CDS
GAGAATCTCTAGTCTGTGTT+TGG 0.350721 8.3:+9892254 None:intergenic
TTCATCAATCTTCCATCAAT+TGG 0.355382 8.3:+9891910 None:intergenic
ACAGAATTCCTTGTAATAGT+TGG 0.363635 8.3:+9891763 None:intergenic
AGCAGAACAATGCCATATTC+AGG 0.374090 8.3:-9892000 MS.gene073962:CDS
TTCCAACTGAAGCTCATTGC+GGG 0.374523 8.3:+9892329 None:intergenic
TCAAAATCAAAATCACCTTT+GGG 0.389288 8.3:+9891697 None:intergenic
TCCAAGAGTATCTGGTCCTT+TGG 0.396596 8.3:-9891815 MS.gene073962:CDS
TCATGCATTCTTCCATCTAA+TGG 0.401833 8.3:+9891976 None:intergenic
AAGTTATCAGGACCAATTGA+TGG 0.410093 8.3:-9891922 MS.gene073962:CDS
CCATATTCAGGACCATTAGA+TGG 0.411197 8.3:-9891988 MS.gene073962:CDS
ACCAAGAGTCCAAGAGTATC+TGG 0.415806 8.3:-9891823 MS.gene073962:CDS
ATAGAAGTCCATTAAGAAGT+AGG 0.426654 8.3:-9891864 MS.gene073962:CDS
ACCAGATACTCTTGGACTCT+TGG 0.427252 8.3:+9891822 None:intergenic
ATTGTTCTGCTTCTATACAT+AGG 0.436227 8.3:+9892012 None:intergenic
AAGAATGCATGAGCAGAAAA+TGG 0.442330 8.3:-9891965 MS.gene073962:CDS
TGGTTCTACACGAACCGAAT+TGG 0.445561 8.3:-9892089 MS.gene073962:CDS
TGTTGAAACTGATAGCCCTA+TGG 0.446699 8.3:-9891743 MS.gene073962:CDS
TTAAGAAGTAGGGCTATTGA+TGG 0.487712 8.3:-9891853 MS.gene073962:CDS
TCGTGTAGAACCACAGGTAA+AGG 0.487940 8.3:+9892099 None:intergenic
CATAATCATATATGAGTGAA+TGG 0.492231 8.3:+9892364 None:intergenic
CCATGCTTTGTAGAAACTCA+TGG 0.497469 8.3:+9892289 None:intergenic
TTGATGATGAACATTCACTT+TGG 0.513853 8.3:-9891678 MS.gene073962:CDS
GTTGAAACTGATAGCCCTAT+GGG 0.514418 8.3:-9891742 MS.gene073962:CDS
CCATGAGTTTCTACAAAGCA+TGG 0.527981 8.3:-9892289 MS.gene073962:CDS
TAGAAGTCCATTAAGAAGTA+GGG 0.530916 8.3:-9891863 MS.gene073962:CDS
TCCAAAGGACCAGATACTCT+TGG 0.532457 8.3:+9891814 None:intergenic
TTCCCGCAATGAGCTTCAGT+TGG 0.551524 8.3:-9892331 MS.gene073962:CDS
TTCGGTTCGTGTAGAACCAC+AGG 0.553276 8.3:+9892093 None:intergenic
TAAGGACTACTATAACCCAT+AGG 0.553316 8.3:+9891727 None:intergenic
CATGAGTTTCTACAAAGCAT+GGG 0.601787 8.3:-9892288 MS.gene073962:CDS
CTTCCAACTGAAGCTCATTG+CGG 0.606732 8.3:+9892328 None:intergenic
ATTGATGAATGAGAGAATGA+TGG 0.606802 8.3:-9891896 MS.gene073962:CDS
AAGGACTACTATAACCCATA+GGG 0.607941 8.3:+9891728 None:intergenic
TTAAGATCTTGAAAGAGTGT+AGG 0.613928 8.3:+9891655 None:intergenic
TAACCATGAAAACCAGTCCA+TGG 0.615491 8.3:+9892404 None:intergenic
AGTCCTTATAATAAACCCAA+AGG 0.617768 8.3:-9891712 MS.gene073962:CDS
ATGGAGAGGAGCATTGGTCA+AGG 0.622754 8.3:-9892052 MS.gene073962:CDS
GCTCATTGCGGGAAAAGATG+AGG 0.624567 8.3:+9892340 None:intergenic
ACGAACCGAATTGGTACCAA+GGG 0.628752 8.3:-9892080 MS.gene073962:CDS
ATAGAAGTCCAACTATTACA+AGG 0.630633 8.3:-9891771 MS.gene073962:CDS
CACGAACCGAATTGGTACCA+AGG 0.659621 8.3:-9892081 MS.gene073962:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTCAATGCTTTATTATTT+TGG + chr8.3:9892109-9892128 None:intergenic 20.0%
!!! TTTTTTTAGACACTTTTTGA+TGG + chr8.3:9891880-9891899 None:intergenic 20.0%
! ATCAAAATCAAAATCACCTT+TGG + chr8.3:9892354-9892373 None:intergenic 25.0%
! CATAATCATATATGAGTGAA+TGG + chr8.3:9891686-9891705 None:intergenic 25.0%
! CATTCACTCATATATGATTA+TGG - chr8.3:9891684-9891703 MS.gene073962:CDS 25.0%
! TCAAAATCAAAATCACCTTT+GGG + chr8.3:9892353-9892372 None:intergenic 25.0%
!!! AATAAAGCATTGAAGTTATC+AGG - chr8.3:9892113-9892132 MS.gene073962:CDS 25.0%
ACAGAATTCCTTGTAATAGT+TGG + chr8.3:9892287-9892306 None:intergenic 30.0%
AGTCCTTATAATAAACCCAA+AGG - chr8.3:9892335-9892354 MS.gene073962:CDS 30.0%
ATAGAAGTCCAACTATTACA+AGG - chr8.3:9892276-9892295 MS.gene073962:CDS 30.0%
ATAGAAGTCCATTAAGAAGT+AGG - chr8.3:9892183-9892202 MS.gene073962:CDS 30.0%
ATTGATGAATGAGAGAATGA+TGG - chr8.3:9892151-9892170 MS.gene073962:CDS 30.0%
ATTGTTCTGCTTCTATACAT+AGG + chr8.3:9892038-9892057 None:intergenic 30.0%
TAGAAGTCCATTAAGAAGTA+GGG - chr8.3:9892184-9892203 MS.gene073962:CDS 30.0%
TTCATCAATCTTCCATCAAT+TGG + chr8.3:9892140-9892159 None:intergenic 30.0%
! TCACCTTTGGGTTTATTATA+AGG + chr8.3:9892341-9892360 None:intergenic 30.0%
! TTAAGATCTTGAAAGAGTGT+AGG + chr8.3:9892395-9892414 None:intergenic 30.0%
!! AACATGATTTTAGCATCCAA+AGG + chr8.3:9892251-9892270 None:intergenic 30.0%
!! TTGATGATGAACATTCACTT+TGG - chr8.3:9892369-9892388 MS.gene073962:CDS 30.0%
AAGAATGCATGAGCAGAAAA+TGG - chr8.3:9892082-9892101 MS.gene073962:CDS 35.0%
AAGGACTACTATAACCCATA+GGG + chr8.3:9892322-9892341 None:intergenic 35.0%
AAGTTATCAGGACCAATTGA+TGG - chr8.3:9892125-9892144 MS.gene073962:CDS 35.0%
CATGAGTTTCTACAAAGCAT+GGG - chr8.3:9891759-9891778 MS.gene073962:CDS 35.0%
TAAGGACTACTATAACCCAT+AGG + chr8.3:9892323-9892342 None:intergenic 35.0%
TCAATAGCCCTACTTCTTAA+TGG + chr8.3:9892194-9892213 None:intergenic 35.0%
TCATGCATTCTTCCATCTAA+TGG + chr8.3:9892074-9892093 None:intergenic 35.0%
TTAAGAAGTAGGGCTATTGA+TGG - chr8.3:9892194-9892213 MS.gene073962:CDS 35.0%
TTACGACCAAAAAACCTTTC+TGG - chr8.3:9891852-9891871 MS.gene073962:CDS 35.0%
!! ACTTTTTGATGGTACCAGAA+AGG + chr8.3:9891869-9891888 None:intergenic 35.0%
!!! ATGGTACCAGAAAGGTTTTT+TGG + chr8.3:9891861-9891880 None:intergenic 35.0%
!!! GAAAGGTTTTTTGGTCGTAA+TGG + chr8.3:9891852-9891871 None:intergenic 35.0%
AGCAGAACAATGCCATATTC+AGG - chr8.3:9892047-9892066 MS.gene073962:CDS 40.0%
ATTGGTACCAAGGGCAAAAA+TGG - chr8.3:9891976-9891995 MS.gene073962:CDS 40.0%
CCATATTCAGGACCATTAGA+TGG - chr8.3:9892059-9892078 MS.gene073962:CDS 40.0%
CCATCTAATGGTCCTGAATA+TGG + chr8.3:9892062-9892081 None:intergenic 40.0%
CCATGAGTTTCTACAAAGCA+TGG - chr8.3:9891758-9891777 MS.gene073962:CDS 40.0%
CCATGCTTTGTAGAAACTCA+TGG + chr8.3:9891761-9891780 None:intergenic 40.0%
GAAAAACATGCCTTTACCTG+TGG - chr8.3:9891938-9891957 MS.gene073962:CDS 40.0%
GAGAATCTCTAGTCTGTGTT+TGG + chr8.3:9891796-9891815 None:intergenic 40.0%
GTTGAAACTGATAGCCCTAT+GGG - chr8.3:9892305-9892324 MS.gene073962:CDS 40.0%
TGTTGAAACTGATAGCCCTA+TGG - chr8.3:9892304-9892323 MS.gene073962:CDS 40.0%
! TTTTGCCCTTGGTACCAATT+CGG + chr8.3:9891975-9891994 None:intergenic 40.0%
ACCAGATACTCTTGGACTCT+TGG + chr8.3:9892228-9892247 None:intergenic 45.0%
ACGAACCGAATTGGTACCAA+GGG - chr8.3:9891967-9891986 MS.gene073962:CDS 45.0%
CTTCCAACTGAAGCTCATTG+CGG + chr8.3:9891722-9891741 None:intergenic 45.0%
TACCAAGGGCAAAAATGGAG+AGG - chr8.3:9891981-9892000 MS.gene073962:CDS 45.0%
TCCAAAGGACCAGATACTCT+TGG + chr8.3:9892236-9892255 None:intergenic 45.0%
TCGTGTAGAACCACAGGTAA+AGG + chr8.3:9891951-9891970 None:intergenic 45.0%
TTCCAACTGAAGCTCATTGC+GGG + chr8.3:9891721-9891740 None:intergenic 45.0%
! GCAAAAATGGAGAGGAGCAT+TGG - chr8.3:9891989-9892008 MS.gene073962:CDS 45.0%
! TGGTTCTACACGAACCGAAT+TGG - chr8.3:9891958-9891977 MS.gene073962:CDS 45.0%
!! ACCAAGAGTCCAAGAGTATC+TGG - chr8.3:9892224-9892243 MS.gene073962:CDS 45.0%
!! TCCAAGAGTATCTGGTCCTT+TGG - chr8.3:9892232-9892251 MS.gene073962:CDS 45.0%
CACGAACCGAATTGGTACCA+AGG - chr8.3:9891966-9891985 MS.gene073962:CDS 50.0%
GCTCATTGCGGGAAAAGATG+AGG + chr8.3:9891710-9891729 None:intergenic 50.0%
TTCCCGCAATGAGCTTCAGT+TGG - chr8.3:9891716-9891735 MS.gene073962:CDS 50.0%
! TTCGGTTCGTGTAGAACCAC+AGG + chr8.3:9891957-9891976 None:intergenic 50.0%
!! ATGGAGAGGAGCATTGGTCA+AGG - chr8.3:9891995-9892014 MS.gene073962:CDS 50.0%
!! CTCCTCTCCATTTTTGCCCT+TGG + chr8.3:9891986-9892005 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 9891645 9892424 9891645 ID=MS.gene073962
chr8.3 mRNA 9891645 9892424 9891645 ID=MS.gene073962.t1;Parent=MS.gene073962
chr8.3 exon 9891645 9892424 9891645 ID=MS.gene073962.t1.exon1;Parent=MS.gene073962.t1
chr8.3 CDS 9891645 9892424 9891645 ID=cds.MS.gene073962.t1;Parent=MS.gene073962.t1
Gene Sequence

>MS.gene073962

ATGGACTGGTTTTCATGGTTATCAAAAACAACTCTTGACCATTCACTCATATATGATTATGGCCTCATCTTTTCCCGCAATGAGCTTCAGTTGGAAGATGCAACTTACTTCAACCATGAGTTTCTACAAAGCATGGGAATCTCAATAGCCAAACACAGACTAGAGATTCTCAAACTAGTCAAAAAAGAACAAGAACTTGATCAACCATTACGACCAAAAAACCTTTCTGGTACCATCAAAAAGTGTCTAAAAAAATGCATGAGCAAGTTTGTTTTTCGCGACGATAGTGTTAAGAAAAACATGCCTTTACCTGTGGTTCTACACGAACCGAATTGGTACCAAGGGCAAAAATGGAGAGGAGCATTGGTCAAGGAAGAGAAGCATCAGAAACCTATGTATAGAAGCAGAACAATGCCATATTCAGGACCATTAGATGGAAGAATGCATGAGCAGAAAATGGTCCAAAATAATAAAGCATTGAAGTTATCAGGACCAATTGATGGAAGATTGATGAATGAGAGAATGATGGTGTATACTAATAGAAGTCCATTAAGAAGTAGGGCTATTGATGGAAGATTCACCAAGAGTCCAAGAGTATCTGGTCCTTTGGATGCTAAAATCATGTTTGAGAATAGAAGTCCAACTATTACAAGGAATTCTGTTGAAACTGATAGCCCTATGGGTTATAGTAGTCCTTATAATAAACCCAAAGGTGATTTTGATTTTGATGATGAACATTCACTTTGGCCTACACTCTTTCAAGATCTTAATCCAACTTGA

Protein sequence

>MS.gene073962.t1

MDWFSWLSKTTLDHSLIYDYGLIFSRNELQLEDATYFNHEFLQSMGISIAKHRLEILKLVKKEQELDQPLRPKNLSGTIKKCLKKCMSKFVFRDDSVKKNMPLPVVLHEPNWYQGQKWRGALVKEEKHQKPMYRSRTMPYSGPLDGRMHEQKMVQNNKALKLSGPIDGRLMNERMMVYTNRSPLRSRAIDGRFTKSPRVSGPLDAKIMFENRSPTITRNSVETDSPMGYSSPYNKPKGDFDFDDEHSLWPTLFQDLNPT