AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29405


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29405.t1 MTR_3g043750 88.776 98 3 1 1 90 1 98 3.84e-58 173
MS.gene29405.t1 MTR_8g041410 58.163 98 32 2 1 89 38 135 9.09e-32 111
MS.gene29405.t1 MTR_5g088770 56.000 100 33 2 1 89 1 100 1.04e-31 107
MS.gene29405.t1 MTR_3g451210 45.745 94 43 2 4 89 31 124 2.17e-24 89.7
MS.gene29405.t1 MTR_3g452280 45.745 94 43 2 4 89 31 124 2.17e-24 89.7
MS.gene29405.t1 MTR_4g133540 40.000 70 42 0 16 85 47 116 1.05e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29405.t1 AT3G12490 59.794 97 31 1 1 89 1 97 5.34e-36 119
MS.gene29405.t1 AT3G12490 59.794 97 31 1 1 89 34 130 4.81e-35 119
MS.gene29405.t1 AT2G40880 56.322 87 30 1 4 82 35 121 4.21e-30 103
MS.gene29405.t1 AT5G12140 50.549 91 37 1 4 86 10 100 5.26e-27 95.1
MS.gene29405.t1 AT5G05110 48.864 88 36 2 4 82 45 132 1.68e-19 79.3

Find 21 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGAGTTCGCGATGTTTC+AGG 0.215517 3.3:+31667697 MS.gene29405:CDS
TGTTATGCAGAATTCACTTC+TGG 0.302530 3.3:+31669084 MS.gene29405:intron
AAAAGATGGGGTGAATAAAA+AGG 0.330688 3.3:+31669177 MS.gene29405:CDS
CATCGATCGCAAGACTGTTC+TGG 0.359811 3.3:-31667723 None:intergenic
CATACACCACATCACTTTAG+AGG 0.393728 3.3:+31669153 MS.gene29405:CDS
GGTTTATGAAGCCAAGGTTT+GGG 0.416419 3.3:+31669198 MS.gene29405:CDS
AGGTTTATGAAGCCAAGGTT+TGG 0.435602 3.3:+31669197 MS.gene29405:CDS
CCAAGGTTTGGGAAAAGTCT+TGG 0.461362 3.3:+31669209 MS.gene29405:CDS
GCTAAAGACCAAGTGGTTGC+TGG 0.469565 3.3:+31669127 MS.gene29405:CDS
ATCACTTTAGAGGTAAAAGA+TGG 0.471008 3.3:+31669163 MS.gene29405:CDS
AGTTAAGCGCTTTACTGCGC+AGG 0.497060 3.3:-31669277 None:intergenic
CTCTAAAGTGATGTGGTGTA+TGG 0.517466 3.3:-31669152 None:intergenic
AAAGTCTTGGATGAACTTCA+AGG 0.534205 3.3:+31669222 MS.gene29405:CDS
AGAAGAATGGCAACGCTTGG+TGG 0.543963 3.3:+31667679 None:intergenic
AGAAGAAGAATGGCAACGCT+TGG 0.566845 3.3:+31667676 None:intergenic
TCACTTTAGAGGTAAAAGAT+GGG 0.584369 3.3:+31669164 MS.gene29405:CDS
GTATGGTACCAGCAACCACT+TGG 0.591179 3.3:-31669135 None:intergenic
CAGAACAGTCTTGCGATCGA+TGG 0.591248 3.3:+31667724 MS.gene29405:CDS
CACTTTAGAGGTAAAAGATG+GGG 0.625879 3.3:+31669165 MS.gene29405:CDS
GATAAGTGCTAAAGACCAAG+TGG 0.690500 3.3:+31669120 MS.gene29405:CDS
GTCTTGGATGAACTTCAAGG+AGG 0.758137 3.3:+31669225 MS.gene29405:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAACAAAATAAATAAATAAA+CGG - chr3.3:31668974-31668993 None:intergenic 10.0%
!! TATTTACTAAAAAAATTACA+AGG - chr3.3:31667892-31667911 None:intergenic 10.0%
!!! AATTTTGTCAAAAAAAAAAA+GGG - chr3.3:31668080-31668099 None:intergenic 10.0%
!!! ATTTTAAAGAATATGATATA+AGG - chr3.3:31668479-31668498 None:intergenic 10.0%
!!! CTTATTTTTTTGATTTTTTT+AGG + chr3.3:31668123-31668142 MS.gene29405:intron 10.0%
!!! TAATTTTGTCAAAAAAAAAA+AGG - chr3.3:31668081-31668100 None:intergenic 10.0%
!!! TTTTATCATTTTTATTTAGT+TGG + chr3.3:31669057-31669076 MS.gene29405:intron 10.0%
!! ACTTTATAAATGTTTCAAAA+AGG - chr3.3:31668534-31668553 None:intergenic 15.0%
!! ATAATAAGTGGTTAAATATA+TGG - chr3.3:31668383-31668402 None:intergenic 15.0%
!!! ATTTTGAGAAATACTAAAAT+TGG + chr3.3:31668778-31668797 MS.gene29405:intron 15.0%
!!! ATTTTGTCAAAAAAAAAAAG+GGG - chr3.3:31668079-31668098 None:intergenic 15.0%
!!! TCTCAAATTTTTAATTAGAA+AGG + chr3.3:31668674-31668693 MS.gene29405:intron 15.0%
!!! TTTTTCTATTGTGAAAAATT+GGG + chr3.3:31668153-31668172 MS.gene29405:intron 15.0%
!!! TTTTTTCTATTGTGAAAAAT+TGG + chr3.3:31668152-31668171 MS.gene29405:intron 15.0%
!! ATAAATAAATAAACGGCTTT+AGG - chr3.3:31668967-31668986 None:intergenic 20.0%
!! TTAACAACTTACTCTTAATT+AGG + chr3.3:31668421-31668440 MS.gene29405:intron 20.0%
!! TTGAAACATTTATAAAGTCA+AGG + chr3.3:31668536-31668555 MS.gene29405:intron 20.0%
!!! AATACTAAAATTGGTTTTTG+AGG + chr3.3:31668787-31668806 MS.gene29405:intron 20.0%
!!! GCAATTTTCTTTAGTTTTTT+TGG + chr3.3:31669019-31669038 MS.gene29405:intron 20.0%
!!! TTTTGTCAAAAAAAAAAAGG+GGG - chr3.3:31668078-31668097 None:intergenic 20.0%
! AAAATAAGTAAAGACCCAAT+TGG - chr3.3:31668111-31668130 None:intergenic 25.0%
! ACAATGAATACTATATGAAG+GGG + chr3.3:31668220-31668239 MS.gene29405:intron 25.0%
! ACGCTACATGATAAAAAAAT+TGG - chr3.3:31667948-31667967 None:intergenic 25.0%
! ATACAAAAATCATGCGTATT+TGG - chr3.3:31667864-31667883 None:intergenic 25.0%
! CAATTGTTGAATCTATTGAT+TGG + chr3.3:31668628-31668647 MS.gene29405:intron 25.0%
! CGCTACATGATAAAAAAATT+GGG - chr3.3:31667947-31667966 None:intergenic 25.0%
! GACAATGAATACTATATGAA+GGG + chr3.3:31668219-31668238 MS.gene29405:intron 25.0%
! TACAAAAATCATGCGTATTT+GGG - chr3.3:31667863-31667882 None:intergenic 25.0%
! TATCCACTTTCTATCAATTT+AGG + chr3.3:31668453-31668472 MS.gene29405:intron 25.0%
! TTATGAGTAGTATGTCAAAA+TGG + chr3.3:31668328-31668347 MS.gene29405:intron 25.0%
!! GAGACTTAATTGATAATAAG+TGG - chr3.3:31668395-31668414 None:intergenic 25.0%
AAAAGATGGGGTGAATAAAA+AGG + chr3.3:31669177-31669196 MS.gene29405:CDS 30.0%
AAAATTACGAGAGACCAATT+GGG + chr3.3:31668094-31668113 MS.gene29405:intron 30.0%
AAAGAGTGAAGTTACATTCA+AGG - chr3.3:31668909-31668928 None:intergenic 30.0%
ACTTGACACTTTAATAGAGT+CGG - chr3.3:31668356-31668375 None:intergenic 30.0%
CGACAATGAATACTATATGA+AGG + chr3.3:31668218-31668237 MS.gene29405:intron 30.0%
TAAAAAGGTTTATGAAGCCA+AGG + chr3.3:31669192-31669211 MS.gene29405:CDS 30.0%
TCTTTCAGTACTAAATGACT+TGG + chr3.3:31668924-31668943 MS.gene29405:intron 30.0%
TGAAGAACACAACAAAAAAC+AGG + chr3.3:31667765-31667784 MS.gene29405:intron 30.0%
TGTCAAGTAACCTAATAAGT+GGG - chr3.3:31668268-31668287 None:intergenic 30.0%
TTGTCAAGTAACCTAATAAG+TGG - chr3.3:31668269-31668288 None:intergenic 30.0%
TTTGGGAAAAAAACGATCAT+CGG - chr3.3:31667846-31667865 None:intergenic 30.0%
! ACTCTATTAAAGTGTCAAGT+TGG + chr3.3:31668356-31668375 MS.gene29405:intron 30.0%
! ATCACTTTAGAGGTAAAAGA+TGG + chr3.3:31669163-31669182 MS.gene29405:CDS 30.0%
! TCACTTTAGAGGTAAAAGAT+GGG + chr3.3:31669164-31669183 MS.gene29405:CDS 30.0%
! TGGTTTTCTCGGTAAAAATA+TGG + chr3.3:31668586-31668605 MS.gene29405:intron 30.0%
!!! AAATAAACGGCTTTAGGTTT+TGG - chr3.3:31668961-31668980 None:intergenic 30.0%
!!! ATTCACTTCTGGAATTTTCA+AGG + chr3.3:31669095-31669114 MS.gene29405:CDS 30.0%
!!! TTCACTTCTGGAATTTTCAA+GGG + chr3.3:31669096-31669115 MS.gene29405:CDS 30.0%
!!! TTTTTTATCATGTAGCGTAG+TGG + chr3.3:31667950-31667969 MS.gene29405:intron 30.0%
AAAGTCTTGGATGAACTTCA+AGG + chr3.3:31669222-31669241 MS.gene29405:CDS 35.0%
AATATGGAGAGCTATACTCA+AGG + chr3.3:31668038-31668057 MS.gene29405:intron 35.0%
AATTTGAGAGATCTCACTGA+AGG - chr3.3:31668663-31668682 None:intergenic 35.0%
ACATACTACTCATAACCTCA+AGG - chr3.3:31668323-31668342 None:intergenic 35.0%
AGGCCTAAATTGATAGAAAG+TGG - chr3.3:31668459-31668478 None:intergenic 35.0%
ATATGGAGAGCTATACTCAA+GGG + chr3.3:31668039-31668058 MS.gene29405:intron 35.0%
CAAAATTACGAGAGACCAAT+TGG + chr3.3:31668093-31668112 MS.gene29405:intron 35.0%
CACCTCTTAAAGTGAATAAG+TGG + chr3.3:31667982-31668001 MS.gene29405:intron 35.0%
CCAACACTACATTGTTTCAA+TGG - chr3.3:31668740-31668759 None:intergenic 35.0%
CCATTGAAACAATGTAGTGT+TGG + chr3.3:31668737-31668756 MS.gene29405:intron 35.0%
CTCCACTTATTCACTTTAAG+AGG - chr3.3:31667987-31668006 None:intergenic 35.0%
CTTGACACTTTAATAGAGTC+GGG - chr3.3:31668355-31668374 None:intergenic 35.0%
GTCAAGTAACCTAATAAGTG+GGG - chr3.3:31668267-31668286 None:intergenic 35.0%
TGTATAAAGTTTACGATGCG+AGG - chr3.3:31668846-31668865 None:intergenic 35.0%
TGTTATGCAGAATTCACTTC+TGG + chr3.3:31669084-31669103 MS.gene29405:intron 35.0%
TGTTCCTTAAACAAGATCAC+AGG - chr3.3:31668706-31668725 None:intergenic 35.0%
! CACTTTAGAGGTAAAAGATG+GGG + chr3.3:31669165-31669184 MS.gene29405:CDS 35.0%
! GTTGAATCTATTGATTGGTC+TGG + chr3.3:31668633-31668652 MS.gene29405:intron 35.0%
!! CTTTAGGTTTTGGTAAGAGA+CGG - chr3.3:31668951-31668970 None:intergenic 35.0%
!!! CTTTTACCTCTAAAGTGATG+TGG - chr3.3:31669162-31669181 None:intergenic 35.0%
AATACTATATGAAGGGGCTG+AGG + chr3.3:31668226-31668245 MS.gene29405:intron 40.0%
ACCAACTGAGCTAAACTCAT+GGG - chr3.3:31668188-31668207 None:intergenic 40.0%
ACTTTGACACCCCACTTATT+AGG + chr3.3:31668255-31668274 MS.gene29405:intron 40.0%
AGGTTTATGAAGCCAAGGTT+TGG + chr3.3:31669197-31669216 MS.gene29405:CDS 40.0%
ATAACCTCAAGGACCTACTT+CGG - chr3.3:31668312-31668331 None:intergenic 40.0%
CATACACCACATCACTTTAG+AGG + chr3.3:31669153-31669172 MS.gene29405:CDS 40.0%
GACTGCTAGATTCATACAAG+AGG + chr3.3:31668883-31668902 MS.gene29405:intron 40.0%
GAGTATAGCTCTCCATATTG+CGG - chr3.3:31668037-31668056 None:intergenic 40.0%
GATAAGTGCTAAAGACCAAG+TGG + chr3.3:31669120-31669139 MS.gene29405:CDS 40.0%
GGTTTATGAAGCCAAGGTTT+GGG + chr3.3:31669198-31669217 MS.gene29405:CDS 40.0%
TACCAACTGAGCTAAACTCA+TGG - chr3.3:31668189-31668208 None:intergenic 40.0%
! CGAACCTGTGATCTTGTTTA+AGG + chr3.3:31668699-31668718 MS.gene29405:intron 40.0%
!! CTCTAAAGTGATGTGGTGTA+TGG - chr3.3:31669155-31669174 None:intergenic 40.0%
!! GAATCTATTGATTGGTCTGG+AGG + chr3.3:31668636-31668655 MS.gene29405:intron 40.0%
CCAACTGAGCTAAACTCATG+GGG - chr3.3:31668187-31668206 None:intergenic 45.0%
CCAAGGTTTGGGAAAAGTCT+TGG + chr3.3:31669209-31669228 MS.gene29405:CDS 45.0%
CGAGAAAACCACCAATTGCA+GGG - chr3.3:31668577-31668596 None:intergenic 45.0%
GTCTTGGATGAACTTCAAGG+AGG + chr3.3:31669225-31669244 MS.gene29405:CDS 45.0%
TACGCTTGCATACCGCAATA+TGG + chr3.3:31668022-31668041 MS.gene29405:intron 45.0%
! ATTGCAGGGCACTCTTACAT+AGG - chr3.3:31668563-31668582 None:intergenic 45.0%
! CCAAGACTTTTCCCAAACCT+TGG - chr3.3:31669212-31669231 None:intergenic 45.0%
!! ATGTAAGAGTGCCCTGCAAT+TGG + chr3.3:31668563-31668582 MS.gene29405:intron 45.0%
!! ATTGAGTTGACCTCCGAAGT+AGG + chr3.3:31668296-31668315 MS.gene29405:intron 45.0%
!! CCTGCAATTGGTGGTTTTCT+CGG + chr3.3:31668575-31668594 MS.gene29405:intron 45.0%
!!! TTATTTTTTTGATTTTTTTA+GGG + chr3.3:31668124-31668143 MS.gene29405:intron 5.0%
CAGAACAGTCTTGCGATCGA+TGG + chr3.3:31667724-31667743 MS.gene29405:CDS 50.0%
CATCGATCGCAAGACTGTTC+TGG - chr3.3:31667726-31667745 None:intergenic 50.0%
CCGAGAAAACCACCAATTGC+AGG - chr3.3:31668578-31668597 None:intergenic 50.0%
CTGAGCTAAACTCATGGGGA+CGG - chr3.3:31668183-31668202 None:intergenic 50.0%
GCTAAAGACCAAGTGGTTGC+TGG + chr3.3:31669127-31669146 MS.gene29405:CDS 50.0%
GTATGGTACCAGCAACCACT+TGG - chr3.3:31669138-31669157 None:intergenic 50.0%
! CCCCATGAGTTTAGCTCAGT+TGG + chr3.3:31668184-31668203 MS.gene29405:intron 50.0%
!! TAAGAGTGCCCTGCAATTGG+TGG + chr3.3:31668566-31668585 MS.gene29405:intron 50.0%
ACCTCAAGGACCTACTTCGG+AGG - chr3.3:31668309-31668328 None:intergenic 55.0%
ACCTCCGAAGTAGGTCCTTG+AGG + chr3.3:31668305-31668324 MS.gene29405:intron 55.0%
GGTGGAGTTCGCGATGTTTC+AGG + chr3.3:31667697-31667716 MS.gene29405:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 31667685 31669288 31667685 ID=MS.gene29405
chr3.3 mRNA 31667685 31669288 31667685 ID=MS.gene29405.t1;Parent=MS.gene29405
chr3.3 exon 31667685 31667762 31667685 ID=MS.gene29405.t1.exon1;Parent=MS.gene29405.t1
chr3.3 CDS 31667685 31667762 31667685 ID=cds.MS.gene29405.t1;Parent=MS.gene29405.t1
chr3.3 exon 31669094 31669288 31669094 ID=MS.gene29405.t1.exon2;Parent=MS.gene29405.t1
chr3.3 CDS 31669094 31669288 31669094 ID=cds.MS.gene29405.t1;Parent=MS.gene29405.t1
Gene Sequence

>MS.gene29405

ATGGCAACGCTTGGTGGAGTTCGCGATGTTTCAGGAAACCAGAACAGTCTTGCGATCGATGGTCTCGCTCGCTTTGCTGTTGAAGAACACAACAAAAAACAGGTTTCTTTTCTTTTTCTTTCTTTCTTTGTTTTATGTTAATTTCTTATTGCGTCATGCCGATGATCGTTTTTTTCCCAAATACGCATGATTTTTGTATTAGATCCTTGTAATTTTTTTAGTAAATATTCGATTTTGTTCTTCACAATTTTCTCAAAAACCCAATTTTTTTATCATGTAGCGTAGTGGCTAGATATTCACCTCTTAAAGTGAATAAGTGGAGAATTCGTGTTCGAACTACGCTTGCATACCGCAATATGGAGAGCTATACTCAAGGGAACACTAAAAAAGCCCCCTTTTTTTTTTTGACAAAATTACGAGAGACCAATTGGGTCTTTACTTATTTTTTTGATTTTTTTAGGGAGTAGTTTTTTCTATTGTGAAAAATTGGGTTAACCGTCCCCATGAGTTTAGCTCAGTTGGTATGAACAATGCGACAATGAATACTATATGAAGGGGCTGAGGTTTGAAACTTTGACACCCCACTTATTAGGTTACTTGACAAAAAAAAAATTGAGTTGACCTCCGAAGTAGGTCCTTGAGGTTATGAGTAGTATGTCAAAATGGTCCCGACTCTATTAAAGTGTCAAGTTGGTCCATATATTTAACCACTTATTATCAATTAAGTCTCTATATTTTAACAACTTACTCTTAATTAGGTCTTAAGTTTATCCACTTTCTATCAATTTAGGCCTTATATCATATTCTTTAAAATGATTATTACGAGTGAAATTTGAGAGTTAGAGACCTTTTTGAAACATTTATAAAGTCAAGGACCTATGTAAGAGTGCCCTGCAATTGGTGGTTTTCTCGGTAAAAATATGGTCGCTCTGCAAGTAATAAGCAATTGTTGAATCTATTGATTGGTCTGGAGGACCTTCAGTGAGATCTCTCAAATTTTTAATTAGAAAGGTTCGAACCTGTGATCTTGTTTAAGGAACATGAGTCTAGTTCCATTGAAACAATGTAGTGTTGGTTTGTCTAGTTGTATTTTATTTTGAGAAATACTAAAATTGGTTTTTGAGGTTCTTAAATATTCATTTTAGCTTTCAAATTAGTCCTCGCATCGTAAACTTTATACACAAAATACAACAAATTTGACTGCTAGATTCATACAAGAGGCCTTGAATGTAACTTCACTCTTTCAGTACTAAATGACTTGGACCGTCTCTTACCAAAACCTAAAGCCGTTTATTTATTTATTTTGTTCATTTTGTAGTGTATAGATTTCAATTGCAATTTTCTTTAGTTTTTTTGGTGTGTTTTTTTTTTTTTTTATCATTTTTATTTAGTTGGTGATTGTTATGCAGAATTCACTTCTGGAATTTTCAAGGGTGATAAGTGCTAAAGACCAAGTGGTTGCTGGTACCATACACCACATCACTTTAGAGGTAAAAGATGGGGTGAATAAAAAGGTTTATGAAGCCAAGGTTTGGGAAAAGTCTTGGATGAACTTCAAGGAGGTGCAAGAGTTCAAGCTCGTTGAAGATGCGCCTGCGCAGTAA

Protein sequence

>MS.gene29405.t1

MATLGGVRDVSGNQNSLAIDGLARFANSLLEFSRVISAKDQVVAGTIHHITLEVKDGVNKKVYEAKVWEKSWMNFKEVQEFKLVEDAPAQ