AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32655


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32655.t1 MTR_3g106750 95.420 131 3 2 1 130 1 129 2.33e-63 189
MS.gene32655.t1 MTR_3g106740 74.615 130 31 2 1 129 1 129 2.05e-56 172
MS.gene32655.t1 MTR_1g012690 57.325 157 37 4 1 130 1 154 1.94e-50 157
MS.gene32655.t1 MTR_1g012700 56.627 166 34 4 1 130 1 164 2.16e-49 155
MS.gene32655.t1 MTR_1g012710 60.784 153 35 3 1 130 1 151 1.01e-44 143
MS.gene32655.t1 MTR_1g012630 79.762 84 16 1 47 130 128 210 1.74e-41 137
MS.gene32655.t1 MTR_4g108150 50.820 122 56 2 9 130 6 123 6.67e-33 112
MS.gene32655.t1 MTR_4g101260 63.095 84 30 1 47 130 50 132 4.88e-31 107
MS.gene32655.t1 MTR_4g101330 40.588 170 58 6 1 129 1 168 2.60e-29 104
MS.gene32655.t1 MTR_4g101280 56.818 88 36 2 44 130 57 143 9.70e-29 102
MS.gene32655.t1 MTR_2g026760 47.619 126 61 3 4 129 6 126 1.38e-28 101
MS.gene32655.t1 MTR_4g101310 57.647 85 34 2 47 130 51 134 8.93e-28 99.8
MS.gene32655.t1 MTR_4g101270 57.143 84 33 3 47 129 105 186 1.58e-22 87.8
MS.gene32655.t1 MTR_5g012340 47.619 84 41 2 47 129 95 176 1.70e-22 87.4
MS.gene32655.t1 MTR_5g012380 46.429 84 42 2 47 129 88 169 7.90e-22 85.5
MS.gene32655.t1 MTR_2g026930 48.750 80 38 2 50 129 122 198 1.57e-16 72.4
MS.gene32655.t1 MTR_2g026920 48.750 80 38 2 50 129 118 194 1.70e-16 72.4
MS.gene32655.t1 MTR_3g082100 46.512 86 45 1 44 129 251 335 1.26e-15 72.0
MS.gene32655.t1 MTR_2g026775 41.772 79 40 3 54 130 686 760 6.78e-13 64.7
MS.gene32655.t1 MTR_4g115360 48.235 85 41 3 47 130 151 233 2.63e-11 58.9
MS.gene32655.t1 MTR_2g026820 52.778 72 27 4 52 121 542 608 3.67e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32655.t1 AT2G45180 69.048 84 25 1 47 130 52 134 4.20e-34 115
MS.gene32655.t1 AT4G12490 60.000 95 36 2 37 130 89 182 5.62e-32 112
MS.gene32655.t1 AT4G12500 38.764 178 60 4 1 130 1 177 1.85e-31 110
MS.gene32655.t1 AT4G12480 60.920 87 32 2 45 130 83 168 5.27e-31 108
MS.gene32655.t1 AT4G12470 58.889 90 35 2 42 130 73 161 8.28e-31 108
MS.gene32655.t1 AT5G46900 63.736 91 29 2 40 130 41 127 3.19e-30 105
MS.gene32655.t1 AT1G62510 61.176 85 31 2 46 129 66 149 1.55e-29 104
MS.gene32655.t1 AT5G46890 61.053 95 33 3 36 130 37 127 1.19e-28 101
MS.gene32655.t1 AT1G12090 62.195 82 29 2 49 129 57 137 1.30e-28 102
MS.gene32655.t1 AT4G12520 52.893 121 53 3 11 130 12 129 4.78e-28 100
MS.gene32655.t1 AT4G12510 52.893 121 53 3 11 130 12 129 4.78e-28 100
MS.gene32655.t1 AT4G12550 50.000 92 44 2 40 130 21 111 4.52e-26 94.7
MS.gene32655.t1 AT4G00165 47.664 107 51 3 27 129 23 128 5.45e-26 95.1
MS.gene32655.t1 AT4G00165 47.664 107 51 3 27 129 23 128 5.45e-26 95.1
MS.gene32655.t1 AT1G12100 41.129 124 70 3 9 130 10 132 8.98e-26 94.7
MS.gene32655.t1 AT4G22460 48.571 105 49 2 28 128 28 131 2.15e-25 93.6
MS.gene32655.t1 AT4G12530 50.000 86 42 1 44 129 31 115 5.32e-23 87.0
MS.gene32655.t1 AT4G12545 50.000 92 41 3 40 130 21 108 6.03e-23 86.7
MS.gene32655.t1 AT3G22142 52.381 84 37 3 47 129 1397 1478 2.04e-20 86.3
MS.gene32655.t1 AT3G22120 51.190 84 39 2 47 129 267 349 5.72e-19 81.3
MS.gene32655.t1 AT3G22120 51.190 84 39 2 47 129 250 332 1.18e-18 80.1
MS.gene32655.t1 AT2G10940 47.727 88 43 3 44 130 205 290 1.24e-18 79.7
MS.gene32655.t1 AT2G10940 47.727 88 43 3 44 130 205 290 1.24e-18 79.7
MS.gene32655.t1 AT4G15160 49.412 85 40 3 47 129 99 182 4.34e-17 73.9
MS.gene32655.t1 AT4G15160 49.412 85 40 3 47 129 181 264 1.11e-16 74.3
MS.gene32655.t1 AT1G62500 42.529 87 47 3 44 129 209 293 6.54e-15 69.7
MS.gene32655.t1 AT4G22490 37.634 93 54 3 38 129 27 116 8.04e-15 66.2

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAACTTTAAGGTATCTT+TGG 0.166346 3.1:-84596430 None:intergenic
AGCACAAACCCCTAACTTTA+AGG 0.205248 3.1:-84596439 None:intergenic
ATCTTTGGGGCAGTGACCTT+GGG 0.214560 3.1:-84596415 None:intergenic
TTGTCTTTGCACTGCAATTA+AGG 0.231170 3.1:+84596560 MS.gene32655:CDS
AATCAAAGGTTTGGCTGATT+TGG 0.237754 3.1:+84596527 MS.gene32655:CDS
TAAAATGGTGGCAAAGAATT+TGG 0.290687 3.1:-84596301 None:intergenic
CCTAACTTTAAGGTATCTTT+GGG 0.293316 3.1:-84596429 None:intergenic
GAAGGTGTTGGCTTTGGCTT+TGG 0.296299 3.1:-84596390 None:intergenic
TGGGGTGAAGGTGTTGGCTT+TGG 0.321997 3.1:-84596396 None:intergenic
CCCAAAGATACCTTAAAGTT+AGG 0.331524 3.1:+84596429 MS.gene32655:CDS
GTTGGCTTTGGCTTTGGCTT+TGG 0.335137 3.1:-84596384 None:intergenic
TATCTTTGGGGCAGTGACCT+TGG 0.380498 3.1:-84596416 None:intergenic
CCAAAGATACCTTAAAGTTA+GGG 0.385359 3.1:+84596430 MS.gene32655:CDS
AGGATCCAGGTGAGGGTGAT+AGG 0.388310 3.1:-84596612 None:intergenic
GGGCAGTGACCTTGGGGTGA+AGG 0.389809 3.1:-84596408 None:intergenic
AAGGAGAGAGAGTACTAAAA+TGG 0.390388 3.1:-84596316 None:intergenic
ATCACCCTCACCTGGATCCT+TGG 0.406696 3.1:+84596615 MS.gene32655:CDS
GTTGTTGGGAATCCTGCTTC+AGG 0.413980 3.1:+84596486 MS.gene32655:CDS
TTTGGCTTTGGCTTTGGTGA+TGG 0.416452 3.1:-84596378 None:intergenic
CTCAACATTTACTGAGGCTA+AGG 0.420857 3.1:+84596344 MS.gene32655:CDS
TTGACAAGCACCAAGGATCC+AGG 0.457767 3.1:-84596625 None:intergenic
GACTGGTTAATGTTGTTGTT+GGG 0.463209 3.1:+84596472 MS.gene32655:CDS
TTGTGCAATAATCAAAGGTT+TGG 0.466120 3.1:+84596518 MS.gene32655:CDS
GGACTGGTTAATGTTGTTGT+TGG 0.480624 3.1:+84596471 MS.gene32655:CDS
TGACCTTGGGGTGAAGGTGT+TGG 0.502306 3.1:-84596402 None:intergenic
AATTGTTGTGCAATAATCAA+AGG 0.523157 3.1:+84596513 MS.gene32655:CDS
GCAATTAAGGCCAATGTGCT+CGG 0.533371 3.1:+84596573 MS.gene32655:CDS
TCTTTGGGGCAGTGACCTTG+GGG 0.545751 3.1:-84596414 None:intergenic
CCTTAGGCACATTGAAAACC+AGG 0.555069 3.1:-84596660 None:intergenic
CAAAGATACCTTAAAGTTAG+GGG 0.560461 3.1:+84596431 MS.gene32655:CDS
ATGTACCTATCACCCTCACC+TGG 0.591606 3.1:+84596607 MS.gene32655:CDS
CAGTAAATGTTGAGTAAGCA+AGG 0.592270 3.1:-84596335 None:intergenic
TCAACATTTACTGAGGCTAA+GGG 0.596026 3.1:+84596345 MS.gene32655:CDS
CAAGTTTATTCCGAGCACAT+TGG 0.596849 3.1:-84596583 None:intergenic
GGTGGCAAAGAATTTGGTGT+TGG 0.599992 3.1:-84596295 None:intergenic
CAACAATTACTACCTGAAGC+AGG 0.602543 3.1:-84596498 None:intergenic
AGCACCAAGGATCCAGGTGA+GGG 0.612333 3.1:-84596619 None:intergenic
AAGCCAACACCTTCACCCCA+AGG 0.614009 3.1:+84596399 MS.gene32655:CDS
TGCTTACTCAACATTTACTG+AGG 0.622636 3.1:+84596338 MS.gene32655:CDS
GAGAGAGAGTACTAAAATGG+TGG 0.630559 3.1:-84596313 None:intergenic
AAGCACCAAGGATCCAGGTG+AGG 0.645068 3.1:-84596620 None:intergenic
AGGCACATTGAAAACCAGGA+GGG 0.674973 3.1:-84596656 None:intergenic
CTAACTTTAAGGTATCTTTG+GGG 0.708820 3.1:-84596428 None:intergenic
TAGGCACATTGAAAACCAGG+AGG 0.727521 3.1:-84596657 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGTTGTGCAATAATCAA+AGG + chr3.1:84596513-84596532 MS.gene32655:CDS 25.0%
CAAAGATACCTTAAAGTTAG+GGG + chr3.1:84596431-84596450 MS.gene32655:CDS 30.0%
CCAAAGATACCTTAAAGTTA+GGG + chr3.1:84596430-84596449 MS.gene32655:CDS 30.0%
TAAAATGGTGGCAAAGAATT+TGG - chr3.1:84596304-84596323 None:intergenic 30.0%
TTGTGCAATAATCAAAGGTT+TGG + chr3.1:84596518-84596537 MS.gene32655:CDS 30.0%
!! CCTAACTTTAAGGTATCTTT+GGG - chr3.1:84596432-84596451 None:intergenic 30.0%
!! CTAACTTTAAGGTATCTTTG+GGG - chr3.1:84596431-84596450 None:intergenic 30.0%
AATCAAAGGTTTGGCTGATT+TGG + chr3.1:84596527-84596546 MS.gene32655:CDS 35.0%
CAGTAAATGTTGAGTAAGCA+AGG - chr3.1:84596338-84596357 None:intergenic 35.0%
CCCAAAGATACCTTAAAGTT+AGG + chr3.1:84596429-84596448 MS.gene32655:CDS 35.0%
TCAACATTTACTGAGGCTAA+GGG + chr3.1:84596345-84596364 MS.gene32655:CDS 35.0%
TGCTTACTCAACATTTACTG+AGG + chr3.1:84596338-84596357 MS.gene32655:CDS 35.0%
TTGTCTTTGCACTGCAATTA+AGG + chr3.1:84596560-84596579 MS.gene32655:CDS 35.0%
! GACTGGTTAATGTTGTTGTT+GGG + chr3.1:84596472-84596491 MS.gene32655:CDS 35.0%
!! AAGGAGAGAGAGTACTAAAA+TGG - chr3.1:84596319-84596338 None:intergenic 35.0%
!! CCCTAACTTTAAGGTATCTT+TGG - chr3.1:84596433-84596452 None:intergenic 35.0%
!!! TTGTTTTTTGACAAGCACCA+AGG - chr3.1:84596635-84596654 None:intergenic 35.0%
AGCACAAACCCCTAACTTTA+AGG - chr3.1:84596442-84596461 None:intergenic 40.0%
CAACAATTACTACCTGAAGC+AGG - chr3.1:84596501-84596520 None:intergenic 40.0%
CAAGTTTATTCCGAGCACAT+TGG - chr3.1:84596586-84596605 None:intergenic 40.0%
CTCAACATTTACTGAGGCTA+AGG + chr3.1:84596344-84596363 MS.gene32655:CDS 40.0%
TGTCAAAAAACAATCCCTCC+TGG + chr3.1:84596642-84596661 MS.gene32655:CDS 40.0%
!! GAGAGAGAGTACTAAAATGG+TGG - chr3.1:84596316-84596335 None:intergenic 40.0%
!! GGACTGGTTAATGTTGTTGT+TGG + chr3.1:84596471-84596490 MS.gene32655:CDS 40.0%
!!! TTGTGCTGATGTTTTAGGAC+TGG + chr3.1:84596455-84596474 MS.gene32655:CDS 40.0%
AGGCACATTGAAAACCAGGA+GGG - chr3.1:84596659-84596678 None:intergenic 45.0%
GCAATTAAGGCCAATGTGCT+CGG + chr3.1:84596573-84596592 MS.gene32655:CDS 45.0%
TAGGCACATTGAAAACCAGG+AGG - chr3.1:84596660-84596679 None:intergenic 45.0%
!! GGGGTTTGTGCTGATGTTTT+AGG + chr3.1:84596450-84596469 MS.gene32655:CDS 45.0%
!! GGTGGCAAAGAATTTGGTGT+TGG - chr3.1:84596298-84596317 None:intergenic 45.0%
!! TTTGGCTTTGGCTTTGGTGA+TGG - chr3.1:84596381-84596400 None:intergenic 45.0%
ATGTACCTATCACCCTCACC+TGG + chr3.1:84596607-84596626 MS.gene32655:CDS 50.0%
GTTGTTGGGAATCCTGCTTC+AGG + chr3.1:84596486-84596505 MS.gene32655:CDS 50.0%
TTGACAAGCACCAAGGATCC+AGG - chr3.1:84596628-84596647 None:intergenic 50.0%
!! ATCTTTGGGGCAGTGACCTT+GGG - chr3.1:84596418-84596437 None:intergenic 50.0%
!! GAAGGTGTTGGCTTTGGCTT+TGG - chr3.1:84596393-84596412 None:intergenic 50.0%
!! GTTGGCTTTGGCTTTGGCTT+TGG - chr3.1:84596387-84596406 None:intergenic 50.0%
!! TATCTTTGGGGCAGTGACCT+TGG - chr3.1:84596419-84596438 None:intergenic 50.0%
AAGCACCAAGGATCCAGGTG+AGG - chr3.1:84596623-84596642 None:intergenic 55.0%
AAGCCAACACCTTCACCCCA+AGG + chr3.1:84596399-84596418 MS.gene32655:CDS 55.0%
AGCACCAAGGATCCAGGTGA+GGG - chr3.1:84596622-84596641 None:intergenic 55.0%
AGGATCCAGGTGAGGGTGAT+AGG - chr3.1:84596615-84596634 None:intergenic 55.0%
ATCACCCTCACCTGGATCCT+TGG + chr3.1:84596615-84596634 MS.gene32655:CDS 55.0%
! TGACCTTGGGGTGAAGGTGT+TGG - chr3.1:84596405-84596424 None:intergenic 55.0%
!! TCTTTGGGGCAGTGACCTTG+GGG - chr3.1:84596417-84596436 None:intergenic 55.0%
!! TGGGGTGAAGGTGTTGGCTT+TGG - chr3.1:84596399-84596418 None:intergenic 55.0%
GGGCAGTGACCTTGGGGTGA+AGG - chr3.1:84596411-84596430 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84596288 84596680 84596288 ID=MS.gene32655
chr3.1 mRNA 84596288 84596680 84596288 ID=MS.gene32655.t1;Parent=MS.gene32655
chr3.1 exon 84596288 84596680 84596288 ID=MS.gene32655.t1.exon1;Parent=MS.gene32655.t1
chr3.1 CDS 84596288 84596680 84596288 ID=cds.MS.gene32655.t1;Parent=MS.gene32655.t1
Gene Sequence

>MS.gene32655

ATGGCTTCCAACACCAAATTCTTTGCCACCATTTTAGTACTCTCTCTCCTTGCTTACTCAACATTTACTGAGGCTAAGGGTTCATGCTCACCATCACCAAAGCCAAAGCCAAAGCCAACACCTTCACCCCAAGGTCACTGCCCCAAAGATACCTTAAAGTTAGGGGTTTGTGCTGATGTTTTAGGACTGGTTAATGTTGTTGTTGGGAATCCTGCTTCAGGTAGTAATTGTTGTGCAATAATCAAAGGTTTGGCTGATTTGGATGCTGCACTTTGTCTTTGCACTGCAATTAAGGCCAATGTGCTCGGAATAAACTTGAATGTACCTATCACCCTCACCTGGATCCTTGGTGCTTGTCAAAAAACAATCCCTCCTGGTTTTCAATGTGCCTAA

Protein sequence

>MS.gene32655.t1

MASNTKFFATILVLSLLAYSTFTEAKGSCSPSPKPKPKPTPSPQGHCPKDTLKLGVCADVLGLVNVVVGNPASGSNCCAIIKGLADLDAALCLCTAIKANVLGINLNVPITLTWILGACQKTIPPGFQCA