AlfalfaGEDB | MS.gene32655

M. sativa cultivar XinJiangDaYe / MS.gene32655

KEGG: -
GO: -
PFAM: PF14547.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32655.t1	XP_003603363.1	96.2	131	2	2	1	130	1	129	2.70E-47	198

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32655.t1	Q39176	60.9	87	32	2	45	130	83	168	2.8e-24	112.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32655.t1	G7JAH5	96.2	131	2	2	1	130	1	129	2.0e-47	198.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049173	MS.gene32655	0.824255	8.91E-54	-1.69E-46
MS.gene049217	MS.gene32655	0.837213	5.93E-57	-1.69E-46
MS.gene049222	MS.gene32655	0.80492	1.71E-49	-1.69E-46
MS.gene049232	MS.gene32655	0.810037	1.41E-50	-1.69E-46
MS.gene049285	MS.gene32655	0.85344	2.39E-61	-1.69E-46
MS.gene049320	MS.gene32655	0.860783	1.62E-63	-1.69E-46
MS.gene049600	MS.gene32655	0.814309	1.64E-51	-1.69E-46
MS.gene049828	MS.gene32655	0.825195	5.35E-54	-1.69E-46
MS.gene05010	MS.gene32655	0.80363	3.18E-49	-1.69E-46
MS.gene050163	MS.gene32655	0.866598	2.54E-65	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32655.t1	MTR_3g106750	95.420	131	3	2	1	130	1	129	2.33e-63	189
MS.gene32655.t1	MTR_3g106740	74.615	130	31	2	1	129	1	129	2.05e-56	172
MS.gene32655.t1	MTR_1g012690	57.325	157	37	4	1	130	1	154	1.94e-50	157
MS.gene32655.t1	MTR_1g012700	56.627	166	34	4	1	130	1	164	2.16e-49	155
MS.gene32655.t1	MTR_1g012710	60.784	153	35	3	1	130	1	151	1.01e-44	143
MS.gene32655.t1	MTR_1g012630	79.762	84	16	1	47	130	128	210	1.74e-41	137
MS.gene32655.t1	MTR_4g108150	50.820	122	56	2	9	130	6	123	6.67e-33	112
MS.gene32655.t1	MTR_4g101260	63.095	84	30	1	47	130	50	132	4.88e-31	107
MS.gene32655.t1	MTR_4g101330	40.588	170	58	6	1	129	1	168	2.60e-29	104
MS.gene32655.t1	MTR_4g101280	56.818	88	36	2	44	130	57	143	9.70e-29	102

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32655.t1	AT2G45180	69.048	84	25	1	47	130	52	134	4.20e-34	115
MS.gene32655.t1	AT4G12490	60.000	95	36	2	37	130	89	182	5.62e-32	112
MS.gene32655.t1	AT4G12500	38.764	178	60	4	1	130	1	177	1.85e-31	110
MS.gene32655.t1	AT4G12480	60.920	87	32	2	45	130	83	168	5.27e-31	108
MS.gene32655.t1	AT4G12470	58.889	90	35	2	42	130	73	161	8.28e-31	108
MS.gene32655.t1	AT5G46900	63.736	91	29	2	40	130	41	127	3.19e-30	105
MS.gene32655.t1	AT1G62510	61.176	85	31	2	46	129	66	149	1.55e-29	104
MS.gene32655.t1	AT5G46890	61.053	95	33	3	36	130	37	127	1.19e-28	101
MS.gene32655.t1	AT1G12090	62.195	82	29	2	49	129	57	137	1.30e-28	102
MS.gene32655.t1	AT4G12520	52.893	121	53	3	11	130	12	129	4.78e-28	100

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Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCACCAAGGATCCAGGTG+AGG	0.645068	3.1:-84596620	None:intergenic
AAGCCAACACCTTCACCCCA+AGG	0.614009	3.1:+84596399	MS.gene32655:CDS
AAGGAGAGAGAGTACTAAAA+TGG	0.390388	3.1:-84596316	None:intergenic
AATCAAAGGTTTGGCTGATT+TGG	0.237754	3.1:+84596527	MS.gene32655:CDS
AATTGTTGTGCAATAATCAA+AGG	0.523157	3.1:+84596513	MS.gene32655:CDS
AGCACAAACCCCTAACTTTA+AGG	0.205248	3.1:-84596439	None:intergenic
AGCACCAAGGATCCAGGTGA+GGG	0.612333	3.1:-84596619	None:intergenic
AGGATCCAGGTGAGGGTGAT+AGG	0.388310	3.1:-84596612	None:intergenic
AGGCACATTGAAAACCAGGA+GGG	0.674973	3.1:-84596656	None:intergenic
ATCACCCTCACCTGGATCCT+TGG	0.406696	3.1:+84596615	MS.gene32655:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAAGATACCTTAAAGTTAG+GGG	+	chr3.1:84596431-84596450	MS.gene32655:CDS	30.0%
CCAAAGATACCTTAAAGTTA+GGG	+	chr3.1:84596430-84596449	MS.gene32655:CDS	30.0%
TAAAATGGTGGCAAAGAATT+TGG	-	chr3.1:84596304-84596323	None:intergenic	30.0%
TTGTGCAATAATCAAAGGTT+TGG	+	chr3.1:84596518-84596537	MS.gene32655:CDS	30.0%
AATCAAAGGTTTGGCTGATT+TGG	+	chr3.1:84596527-84596546	MS.gene32655:CDS	35.0%
CAGTAAATGTTGAGTAAGCA+AGG	-	chr3.1:84596338-84596357	None:intergenic	35.0%
CCCAAAGATACCTTAAAGTT+AGG	+	chr3.1:84596429-84596448	MS.gene32655:CDS	35.0%
TCAACATTTACTGAGGCTAA+GGG	+	chr3.1:84596345-84596364	MS.gene32655:CDS	35.0%
TGCTTACTCAACATTTACTG+AGG	+	chr3.1:84596338-84596357	MS.gene32655:CDS	35.0%
TTGTCTTTGCACTGCAATTA+AGG	+	chr3.1:84596560-84596579	MS.gene32655:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	84596288	84596680	84596288	ID=MS.gene32655
chr3.1	mRNA	84596288	84596680	84596288	ID=MS.gene32655.t1;Parent=MS.gene32655
chr3.1	exon	84596288	84596680	84596288	ID=MS.gene32655.t1.exon1;Parent=MS.gene32655.t1
chr3.1	CDS	84596288	84596680	84596288	ID=cds.MS.gene32655.t1;Parent=MS.gene32655.t1

Gene Sequence

>MS.gene32655

ATGGCTTCCAACACCAAATTCTTTGCCACCATTTTAGTACTCTCTCTCCTTGCTTACTCAACATTTACTGAGGCTAAGGGTTCATGCTCACCATCACCAAAGCCAAAGCCAAAGCCAACACCTTCACCCCAAGGTCACTGCCCCAAAGATACCTTAAAGTTAGGGGTTTGTGCTGATGTTTTAGGACTGGTTAATGTTGTTGTTGGGAATCCTGCTTCAGGTAGTAATTGTTGTGCAATAATCAAAGGTTTGGCTGATTTGGATGCTGCACTTTGTCTTTGCACTGCAATTAAGGCCAATGTGCTCGGAATAAACTTGAATGTACCTATCACCCTCACCTGGATCCTTGGTGCTTGTCAAAAAACAATCCCTCCTGGTTTTCAATGTGCCTAA

Protein sequence

>MS.gene32655.t1

MASNTKFFATILVLSLLAYSTFTEAKGSCSPSPKPKPKPTPSPQGHCPKDTLKLGVCADVLGLVNVVVGNPASGSNCCAIIKGLADLDAALCLCTAIKANVLGINLNVPITLTWILGACQKTIPPGFQCA