AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38966


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38966.t1 MTR_8g461390 95.089 224 11 0 1 224 4 227 6.39e-163 449
MS.gene38966.t1 MTR_4g105470 41.111 180 97 4 36 212 23 196 9.10e-33 118
MS.gene38966.t1 MTR_8g069755 35.096 208 121 5 10 212 5 203 3.07e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38966.t1 AT1G17100 60.891 202 74 2 22 218 30 231 2.82e-90 265
MS.gene38966.t1 AT1G78450 47.768 224 104 4 1 219 1 216 8.52e-71 216
MS.gene38966.t1 AT1G78460 41.126 231 110 5 1 217 1 219 3.42e-51 166

Find 48 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGTTTGATTTGGAAAA+TGG 0.222937 8.4:-49225856 MS.gene38966:CDS
CTTATCTCAATTTCATTATC+AGG 0.236313 8.4:-49227406 MS.gene38966:CDS
AAGCTACTAGAACTGGTTTC+AGG 0.240236 8.4:-49227237 MS.gene38966:CDS
GACGTTATAATTCGTCTGTT+TGG 0.282437 8.4:-49227297 MS.gene38966:CDS
GGATTTGCTTGGTTCACTTT+TGG 0.290356 8.4:+49226123 None:intergenic
TAGGCTATTTGATTACATTC+AGG 0.303343 8.4:-49226256 MS.gene38966:CDS
GATTTGGTTCTTGTTTGATT+TGG 0.323831 8.4:-49225863 MS.gene38966:CDS
GGGACACTCTATGCGTTTGC+AGG 0.331557 8.4:+49227360 None:intergenic
TGAACATGAAGACCCTTTGC+AGG 0.337320 8.4:+49226096 None:intergenic
AATGTCTTGAATAGGAGAAT+TGG 0.347748 8.4:+49227270 None:intergenic
TATGCAGCAGTTAGACAATT+TGG 0.366995 8.4:-49226057 MS.gene38966:CDS
CTTGTTGAAGCTACTAGAAC+TGG 0.386778 8.4:-49227244 MS.gene38966:CDS
ATAATAGGGTGAATGAGATT+TGG 0.387077 8.4:-49225879 MS.gene38966:CDS
ACAAATGAAGACTCACAAAA+TGG 0.387846 8.4:+49226162 None:intergenic
AGCTTCTATGACATCATAGT+TGG 0.390370 8.4:+49227339 None:intergenic
TTGCAGGAGGTGGATTTGCT+TGG 0.398401 8.4:+49226112 None:intergenic
CTGAAGCATGACCAGCTCTA+TGG 0.399888 8.4:+49225941 None:intergenic
ACCCTATTATCATATTCAAA+AGG 0.401311 8.4:+49225892 None:intergenic
ACAAGAGAAATGTCTTGAAT+AGG 0.403479 8.4:+49227262 None:intergenic
CCCTATTATCATATTCAAAA+GGG 0.409363 8.4:+49225893 None:intergenic
AGGCTATTTGATTACATTCA+GGG 0.450840 8.4:-49226255 MS.gene38966:CDS
TGAGGAAGCTGCAGCATTGA+AGG 0.451680 8.4:-49226007 MS.gene38966:CDS
TGAAGACCCTTTGCAGGAGG+TGG 0.452104 8.4:+49226102 None:intergenic
AACTATGATGTCATAGAAGC+TGG 0.463929 8.4:-49227337 MS.gene38966:CDS
GCATTGAAGGACAGTATTGC+CGG 0.475834 8.4:-49225994 MS.gene38966:CDS
GATGTCATAGAAGCTGGAAA+TGG 0.490076 8.4:-49227331 MS.gene38966:CDS
AGGGTCTTCATGTTCAAAGA+TGG 0.509198 8.4:-49226089 MS.gene38966:CDS
AGCAAATCCACCTCCTGCAA+AGG 0.509519 8.4:-49226109 MS.gene38966:CDS
GCAAATCCACCTCCTGCAAA+GGG 0.510194 8.4:-49226108 MS.gene38966:CDS
GGTAAGACTTCAGAGAGAAC+AGG 0.536541 8.4:+49226195 None:intergenic
GCTTCTATGACATCATAGTT+GGG 0.553146 8.4:+49227340 None:intergenic
TCACAAAATGGTCCATCACT+TGG 0.562399 8.4:+49226174 None:intergenic
TCTGAAGTCTTACCAAGTGA+TGG 0.564116 8.4:-49226186 MS.gene38966:CDS
GCAGCAGTTAGACAATTTGG+TGG 0.569529 8.4:-49226054 MS.gene38966:CDS
GGCTATTTGATTACATTCAG+GGG 0.574090 8.4:-49226254 MS.gene38966:CDS
GGACACTCTATGCGTTTGCA+GGG 0.574179 8.4:+49227361 None:intergenic
TGTTGTGGTGGTTACAGCCA+TGG 0.575258 8.4:+49227453 None:intergenic
TTTGTCAAAGATACAGATGT+TGG 0.582094 8.4:-49226030 MS.gene38966:CDS
AGCAGAAGACCACTTAGTAC+CGG 0.588835 8.4:+49225975 None:intergenic
ATTGAACAAAGCCATAGAGC+TGG 0.606780 8.4:-49225952 MS.gene38966:CDS
ACAGTATTGCCGGTACTAAG+TGG 0.612734 8.4:-49225984 MS.gene38966:CDS
CCTATTATCATATTCAAAAG+GGG 0.627523 8.4:+49225894 None:intergenic
CAAAGATACAGATGTTGGTG+AGG 0.634324 8.4:-49226025 MS.gene38966:CDS
CAGTGAGAGCTTCAATGTTG+TGG 0.636610 8.4:+49227438 None:intergenic
ACATGAAGACCCTTTGCAGG+AGG 0.641553 8.4:+49226099 None:intergenic
TCATGCTTCAGTTTACTCTG+TGG 0.643170 8.4:-49225929 MS.gene38966:CDS
CTACTAGAACTGGTTTCAGG+AGG 0.662955 8.4:-49227234 MS.gene38966:intron
TGAGAGCTTCAATGTTGTGG+TGG 0.673155 8.4:+49227441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTGAATACATATAAAA+GGG - chr8.4:49226299-49226318 MS.gene38966:intron 15.0%
!! AATGTGAAATGATTTATATT+TGG - chr8.4:49226728-49226747 MS.gene38966:intron 15.0%
!!! TTAATCTTTTTCTTTTTCTT+AGG - chr8.4:49227016-49227035 MS.gene38966:intron 15.0%
!! GAAAGTTGAATACATATAAA+AGG - chr8.4:49226298-49226317 MS.gene38966:intron 20.0%
!!! AAAATTAATTGTCTCTAGAA+TGG + chr8.4:49226813-49226832 None:intergenic 20.0%
!!! AAATTAATTGTCTCTAGAAT+GGG + chr8.4:49226812-49226831 None:intergenic 20.0%
!!! AGTTTTTTCAGTAATAGTTT+AGG - chr8.4:49226099-49226118 MS.gene38966:CDS 20.0%
! AACTAATTCTAACTTGAAGT+TGG + chr8.4:49226527-49226546 None:intergenic 25.0%
! AATCAAGTTTAGTAACTCTT+CGG + chr8.4:49226998-49227017 None:intergenic 25.0%
! ACCCTATTATCATATTCAAA+AGG + chr8.4:49227402-49227421 None:intergenic 25.0%
! ATGCTAAAATCAATTGTTTC+TGG - chr8.4:49226851-49226870 MS.gene38966:intron 25.0%
! CCCTATTATCATATTCAAAA+GGG + chr8.4:49227401-49227420 None:intergenic 25.0%
! CCTATTATCATATTCAAAAG+GGG + chr8.4:49227400-49227419 None:intergenic 25.0%
! CTTATCTCAATTTCATTATC+AGG - chr8.4:49225885-49225904 MS.gene38966:CDS 25.0%
! TTTAGTGTAATTACAAGTGT+CGG - chr8.4:49226428-49226447 MS.gene38966:intron 25.0%
! TTTCAAATTATTAGCTGTGT+CGG + chr8.4:49226394-49226413 None:intergenic 25.0%
!! CCCTTTTGAATATGATAATA+GGG - chr8.4:49227398-49227417 MS.gene38966:CDS 25.0%
!!! AAGTTTTAATGAAGTGTTTG+TGG - chr8.4:49226956-49226975 MS.gene38966:intron 25.0%
!!! ACAATTGATTTTAGCATGTT+TGG + chr8.4:49226848-49226867 None:intergenic 25.0%
!!! ACAATTGATTTTGACATGTT+TGG + chr8.4:49226582-49226601 None:intergenic 25.0%
!!! TTCTTGTTTGATTTGGAAAA+TGG - chr8.4:49227435-49227454 MS.gene38966:CDS 25.0%
AATGTCTTGAATAGGAGAAT+TGG + chr8.4:49226024-49226043 None:intergenic 30.0%
ACAAATGAAGACTCACAAAA+TGG + chr8.4:49227132-49227151 None:intergenic 30.0%
ACAAGAGAAATGTCTTGAAT+AGG + chr8.4:49226032-49226051 None:intergenic 30.0%
AGGCTATTTGATTACATTCA+GGG - chr8.4:49227036-49227055 MS.gene38966:intron 30.0%
ATAATAGGGTGAATGAGATT+TGG - chr8.4:49227412-49227431 MS.gene38966:CDS 30.0%
TAGGCTATTTGATTACATTC+AGG - chr8.4:49227035-49227054 MS.gene38966:intron 30.0%
TGAACTTCATGATCATTCAT+TGG + chr8.4:49226922-49226941 None:intergenic 30.0%
TTTGTCAAAGATACAGATGT+TGG - chr8.4:49227261-49227280 MS.gene38966:CDS 30.0%
! CCCCTTTTGAATATGATAAT+AGG - chr8.4:49227397-49227416 MS.gene38966:CDS 30.0%
!! GATTTGGTTCTTGTTTGATT+TGG - chr8.4:49227428-49227447 MS.gene38966:CDS 30.0%
AACTATGATGTCATAGAAGC+TGG - chr8.4:49225954-49225973 MS.gene38966:CDS 35.0%
AAGTTTAGTAACTCTTCGGA+TGG + chr8.4:49226994-49227013 None:intergenic 35.0%
AGCTTCTATGACATCATAGT+TGG + chr8.4:49225955-49225974 None:intergenic 35.0%
AGTTTAGTAACTCTTCGGAT+GGG + chr8.4:49226993-49227012 None:intergenic 35.0%
GACGTTATAATTCGTCTGTT+TGG - chr8.4:49225994-49226013 MS.gene38966:CDS 35.0%
GCTTCTATGACATCATAGTT+GGG + chr8.4:49225954-49225973 None:intergenic 35.0%
GGCTATTTGATTACATTCAG+GGG - chr8.4:49227037-49227056 MS.gene38966:intron 35.0%
TATGCAGCAGTTAGACAATT+TGG - chr8.4:49227234-49227253 MS.gene38966:intron 35.0%
! TTAGTGTGTGTTTGGTTTCA+CGG + chr8.4:49226898-49226917 None:intergenic 35.0%
!! TCATTGGTTTAGTGTGTGTT+TGG + chr8.4:49226906-49226925 None:intergenic 35.0%
!!! CTGTCATCTCAATTTTTTGC+TGG + chr8.4:49227074-49227093 None:intergenic 35.0%
AGGGTCTTCATGTTCAAAGA+TGG - chr8.4:49227202-49227221 MS.gene38966:intron 40.0%
ATTGAACAAAGCCATAGAGC+TGG - chr8.4:49227339-49227358 MS.gene38966:CDS 40.0%
CAAAGATACAGATGTTGGTG+AGG - chr8.4:49227266-49227285 MS.gene38966:CDS 40.0%
CTTGTTGAAGCTACTAGAAC+TGG - chr8.4:49226047-49226066 MS.gene38966:CDS 40.0%
GATGTCATAGAAGCTGGAAA+TGG - chr8.4:49225960-49225979 MS.gene38966:CDS 40.0%
GGGAGTTGAAAAATCATGTC+TGG - chr8.4:49226319-49226338 MS.gene38966:intron 40.0%
GTGTTTGCTTCTAGTGTGAT+TGG - chr8.4:49226213-49226232 MS.gene38966:CDS 40.0%
TATGAAGCACAGACAATCCT+CGG + chr8.4:49226479-49226498 None:intergenic 40.0%
TCACAAAATGGTCCATCACT+TGG + chr8.4:49227120-49227139 None:intergenic 40.0%
TCATGCTTCAGTTTACTCTG+TGG - chr8.4:49227362-49227381 MS.gene38966:CDS 40.0%
! AAGCTACTAGAACTGGTTTC+AGG - chr8.4:49226054-49226073 MS.gene38966:CDS 40.0%
! TAATGAAGTGTTTGTGGTGC+TGG - chr8.4:49226962-49226981 MS.gene38966:intron 40.0%
! TCTGAAGTCTTACCAAGTGA+TGG - chr8.4:49227105-49227124 MS.gene38966:intron 40.0%
!! AATTACAAGTGTCGGTGTGT+CGG - chr8.4:49226436-49226455 MS.gene38966:intron 40.0%
!! GGATTTGCTTGGTTCACTTT+TGG + chr8.4:49227171-49227190 None:intergenic 40.0%
ACACTTCCCAACGTGAAAAG+TGG + chr8.4:49226629-49226648 None:intergenic 45.0%
ACAGTATTGCCGGTACTAAG+TGG - chr8.4:49227307-49227326 MS.gene38966:CDS 45.0%
AGCAGAAGACCACTTAGTAC+CGG + chr8.4:49227319-49227338 None:intergenic 45.0%
CACTTCCCAACGTGAAAAGT+GGG + chr8.4:49226628-49226647 None:intergenic 45.0%
CAGTGAGAGCTTCAATGTTG+TGG + chr8.4:49225856-49225875 None:intergenic 45.0%
GAGGATTGTCTGTGCTTCAT+AGG - chr8.4:49226478-49226497 MS.gene38966:intron 45.0%
GCAGCAGTTAGACAATTTGG+TGG - chr8.4:49227237-49227256 MS.gene38966:CDS 45.0%
GCATTGAAGGACAGTATTGC+CGG - chr8.4:49227297-49227316 MS.gene38966:CDS 45.0%
GGTAAGACTTCAGAGAGAAC+AGG + chr8.4:49227099-49227118 None:intergenic 45.0%
TGAACATGAAGACCCTTTGC+AGG + chr8.4:49227198-49227217 None:intergenic 45.0%
TGAGAGCTTCAATGTTGTGG+TGG + chr8.4:49225853-49225872 None:intergenic 45.0%
TTGTCTGTGCTTCATAGGTC+TGG - chr8.4:49226483-49226502 MS.gene38966:intron 45.0%
! ATGTCTGGAGCTATGAAGCA+CGG - chr8.4:49226334-49226353 MS.gene38966:intron 45.0%
! CTACTAGAACTGGTTTCAGG+AGG - chr8.4:49226057-49226076 MS.gene38966:CDS 45.0%
! TGTGTGTTTGGTTTCACGGT+GGG + chr8.4:49226894-49226913 None:intergenic 45.0%
!! ACTCTTCCCACTTTTCACGT+TGG - chr8.4:49226619-49226638 MS.gene38966:intron 45.0%
!! CTCTTCCCACTTTTCACGTT+GGG - chr8.4:49226620-49226639 MS.gene38966:intron 45.0%
ACATGAAGACCCTTTGCAGG+AGG + chr8.4:49227195-49227214 None:intergenic 50.0%
AGCAAATCCACCTCCTGCAA+AGG - chr8.4:49227182-49227201 MS.gene38966:intron 50.0%
CTGAAGCATGACCAGCTCTA+TGG + chr8.4:49227353-49227372 None:intergenic 50.0%
GCAAATCCACCTCCTGCAAA+GGG - chr8.4:49227183-49227202 MS.gene38966:intron 50.0%
GCACAGACAATCCTCGGATT+AGG + chr8.4:49226473-49226492 None:intergenic 50.0%
GGACACTCTATGCGTTTGCA+GGG + chr8.4:49225933-49225952 None:intergenic 50.0%
! GTGTGTGTTTGGTTTCACGG+TGG + chr8.4:49226895-49226914 None:intergenic 50.0%
! TGAGGAAGCTGCAGCATTGA+AGG - chr8.4:49227284-49227303 MS.gene38966:CDS 50.0%
! TTGCAGGAGGTGGATTTGCT+TGG + chr8.4:49227182-49227201 None:intergenic 50.0%
ATGAAGCACGGATGCGGACA+CGG - chr8.4:49226346-49226365 MS.gene38966:intron 55.0%
GGAGCTATGAAGCACGGATG+CGG - chr8.4:49226340-49226359 MS.gene38966:intron 55.0%
GGGACACTCTATGCGTTTGC+AGG + chr8.4:49225934-49225953 None:intergenic 55.0%
TGAAGACCCTTTGCAGGAGG+TGG + chr8.4:49227192-49227211 None:intergenic 55.0%
TGTCGCATACGCCTAATCCG+AGG - chr8.4:49226459-49226478 MS.gene38966:intron 55.0%
ATGCGGACACGGACACGACA+CGG - chr8.4:49226357-49226376 MS.gene38966:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 49225840 49227473 49225840 ID=MS.gene38966
chr8.4 mRNA 49225840 49227473 49225840 ID=MS.gene38966.t1;Parent=MS.gene38966
chr8.4 exon 49227235 49227473 49227235 ID=MS.gene38966.t1.exon1;Parent=MS.gene38966.t1
chr8.4 CDS 49227235 49227473 49227235 ID=cds.MS.gene38966.t1;Parent=MS.gene38966.t1
chr8.4 exon 49225840 49226275 49225840 ID=MS.gene38966.t1.exon2;Parent=MS.gene38966.t1
chr8.4 CDS 49225840 49226275 49225840 ID=cds.MS.gene38966.t1;Parent=MS.gene38966.t1
Gene Sequence

>MS.gene38966

ATGGCTGTAACCACCACAACATTGAAGCTCTCACTGTTCTTGACTCTTATCTCAATTTCATTATCAGGTATTCTTTCATATGATATTATACCCTGCAAACGCATAGAGTGTCCCAACTATGATGTCATAGAAGCTGGAAATGGCTATGAAATCAGACGTTATAATTCGTCTGTTTGGATTTCCAATTCTCCTATTCAAGACATTTCTCTTGTTGAAGCTACTAGAACTGGTTTCAGGAGGTAATTTGTTTTTTTACTTAAGTTTTTTCAGTAATAGTTTAGGTTTTCGAATTCTATTTTCTATGAGTGCATTTGATTAGTATACAAAAAAATATGATGTTTAACTTGCATGTTTGATTGAAAAAACTAAATGTGTGTTTGCTTCTAGTGTGATTGGAATTGATTTTGAATTAATTGATTTTATTTCAAATAAGCTTATTGTGAAGTGCTTTATGTTTTGAAAGTTGAATACATATAAAAGGGAGTTGAAAAATCATGTCTGGAGCTATGAAGCACGGATGCGGACACGGACACGACACGGACACTGACACGCCGACACAGCTAATAATTTGAAAAAAAAAGCGCATAATTTAGTGTAATTACAAGTGTCGGTGTGTCGGTGTCGCATACGCCTAATCCGAGGATTGTCTGTGCTTCATAGGTCTGGAGTCAAAAGATACTAATTCCAACTTCAAGTTAGAATTAGTTGTAGCGACAGAATCAATTTTGATCGACGTCATCCAAACATGTCAAAATCAATTGTGTCTGAAATCACTTTTGACTCTTCCCACTTTTCACGTTGGGAAGTGTGTTTGCTTTTGCATTGACATAAATTGATTTTTAATGAATTCATTCTGTAAAATTATTGATTTTAATTAAAAGTAAGTTGAATGTGAAATGATTTATATTTGGATACACTCAAGTAAAAATGAGTTGAGCTATAAATTAAGCTTCAAGTTAGAACTTAGAACCCATTCTAGAGACAATTAATTTTTATCGAAATCATCCAAACATGCTAAAATCAATTGTTTCTGGAATCACTTTTGACACTTCCCACCGTGAAACCAAACACACACTAAACCAATGAATGATCATGAAGTTCATCTATACTGCAAAGAAGTTTTAATGAAGTGTTTGTGGTGCTGGATTGACCCATCCGAAGAGTTACTAAACTTGATTAATCTTTTTCTTTTTCTTAGGCTATTTGATTACATTCAGGGGAAGAACAACTACCAGCAAAAAATTGAGATGACAGCACCTGTTCTCTCTGAAGTCTTACCAAGTGATGGACCATTTTGTGAGTCTTCATTTGTTGTCAGCTTTTATGTACCAAAAGTGAACCAAGCAAATCCACCTCCTGCAAAGGGTCTTCATGTTCAAAGATGGAAAACTGTGTATGCAGCAGTTAGACAATTTGGTGGTTTTGTCAAAGATACAGATGTTGGTGAGGAAGCTGCAGCATTGAAGGACAGTATTGCCGGTACTAAGTGGTCTTCTGCTATTGAACAAAGCCATAGAGCTGGTCATGCTTCAGTTTACTCTGTGGCTCAGTACAATGCCCCTTTTGAATATGATAATAGGGTGAATGAGATTTGGTTCTTGTTTGATTTGGAAAATGGATTGCATTCTATGTGA

Protein sequence

>MS.gene38966.t1

MAVTTTTLKLSLFLTLISISLSGILSYDIIPCKRIECPNYDVIEAGNGYEIRRYNSSVWISNSPIQDISLVEATRTGFRRLFDYIQGKNNYQQKIEMTAPVLSEVLPSDGPFCESSFVVSFYVPKVNQANPPPAKGLHVQRWKTVYAAVRQFGGFVKDTDVGEEAAALKDSIAGTKWSSAIEQSHRAGHASVYSVAQYNAPFEYDNRVNEIWFLFDLENGLHSM