Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40648.t1 | XP_024638309.1 | 98.1 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 1.10E-21 | 111.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40648.t1 | Q9XIA7 | 62.3 | 53 | 20 | 0 | 1 | 53 | 1 | 53 | 1.1e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40648.t1 | A0A072UL16 | 98.1 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 7.8e-22 | 111.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene40648 | 0.851384 | 9.19E-61 | -1.69E-46 |
| MS.gene049341 | MS.gene40648 | 0.82456 | 7.55E-54 | -1.69E-46 |
| MS.gene049756 | MS.gene40648 | 0.823842 | 1.11E-53 | -1.69E-46 |
| MS.gene049821 | MS.gene40648 | 0.80189 | 7.27E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene40648 | 0.811603 | 6.45E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene40648 | 0.80247 | 5.52E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene40648 | 0.924953 | 3.53E-90 | -1.69E-46 |
| MS.gene050303 | MS.gene40648 | 0.875552 | 2.82E-68 | -1.69E-46 |
| MS.gene050320 | MS.gene40648 | 0.832423 | 9.53E-56 | -1.69E-46 |
| MS.gene050752 | MS.gene40648 | 0.800782 | 1.23E-48 | -1.69E-46 |
| MS.gene050815 | MS.gene40648 | 0.807826 | 4.18E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene40648 | 0.805318 | 1.41E-49 | -1.69E-46 |
| MS.gene050916 | MS.gene40648 | 0.80991 | 1.50E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene40648 | 0.847147 | 1.39E-59 | -1.69E-46 |
| MS.gene051241 | MS.gene40648 | 0.804819 | 1.80E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene40648 | 0.819369 | 1.20E-52 | -1.69E-46 |
| MS.gene051245 | MS.gene40648 | 0.832162 | 1.11E-55 | -1.69E-46 |
| MS.gene051328 | MS.gene40648 | 0.832615 | 8.54E-56 | -1.69E-46 |
| MS.gene051330 | MS.gene40648 | 0.855002 | 8.45E-62 | -1.69E-46 |
| MS.gene051349 | MS.gene40648 | 0.802464 | 5.54E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene40648 | 0.863562 | 2.28E-64 | -1.69E-46 |
| MS.gene051451 | MS.gene40648 | 0.840242 | 9.78E-58 | -1.69E-46 |
| MS.gene051633 | MS.gene40648 | 0.842498 | 2.49E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene40648 | 0.802684 | 4.99E-49 | -1.69E-46 |
| MS.gene051746 | MS.gene40648 | 0.878361 | 2.99E-69 | -1.69E-46 |
| MS.gene051748 | MS.gene40648 | 0.868068 | 8.59E-66 | -1.69E-46 |
| MS.gene051757 | MS.gene40648 | 0.814711 | 1.34E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene40648 | 0.834415 | 3.04E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene40648 | 0.875957 | 2.04E-68 | -1.69E-46 |
| MS.gene051975 | MS.gene40648 | 0.823572 | 1.29E-53 | -1.69E-46 |
| MS.gene052133 | MS.gene40648 | 0.814984 | 1.17E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene40648 | 0.863211 | 2.93E-64 | -1.69E-46 |
| MS.gene052302 | MS.gene40648 | 0.850922 | 1.24E-60 | -1.69E-46 |
| MS.gene052376 | MS.gene40648 | 0.853113 | 2.96E-61 | -1.69E-46 |
| MS.gene052947 | MS.gene40648 | 0.887669 | 1.16E-72 | -1.69E-46 |
| MS.gene053058 | MS.gene40648 | 0.809813 | 1.57E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene40648 | 0.856739 | 2.62E-62 | -1.69E-46 |
| MS.gene053952 | MS.gene40648 | 0.808793 | 2.60E-50 | -1.69E-46 |
| MS.gene054403 | MS.gene40648 | 0.801343 | 9.42E-49 | -1.69E-46 |
| MS.gene055162 | MS.gene40648 | 0.818319 | 2.09E-52 | -1.69E-46 |
| MS.gene055441 | MS.gene40648 | 0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene40648 | 0.80268 | 5.00E-49 | -1.69E-46 |
| MS.gene055555 | MS.gene40648 | 0.806107 | 9.66E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene40648 | 0.873561 | 1.34E-67 | -1.69E-46 |
| MS.gene056075 | MS.gene40648 | 0.801945 | 7.09E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene40648 | 0.853701 | 2.01E-61 | -1.69E-46 |
| MS.gene056601 | MS.gene40648 | 0.849668 | 2.79E-60 | -1.69E-46 |
| MS.gene056631 | MS.gene40648 | 0.96697 | 1.23E-126 | -1.69E-46 |
| MS.gene056760 | MS.gene40648 | 0.832609 | 8.57E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene40648 | 0.837216 | 5.93E-57 | -1.69E-46 |
| MS.gene057057 | MS.gene40648 | 0.83191 | 1.28E-55 | -1.69E-46 |
| MS.gene057079 | MS.gene40648 | 0.839558 | 1.47E-57 | -1.69E-46 |
| MS.gene057094 | MS.gene40648 | 0.839254 | 1.77E-57 | -1.69E-46 |
| MS.gene057140 | MS.gene40648 | 0.844776 | 6.13E-59 | -1.69E-46 |
| MS.gene057234 | MS.gene40648 | 0.847517 | 1.10E-59 | -1.69E-46 |
| MS.gene057693 | MS.gene40648 | 0.802317 | 5.94E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene40648 | 0.86104 | 1.36E-63 | -1.69E-46 |
| MS.gene05795 | MS.gene40648 | 0.82998 | 3.80E-55 | -1.69E-46 |
| MS.gene057999 | MS.gene40648 | 0.800508 | 1.39E-48 | -1.69E-46 |
| MS.gene058055 | MS.gene40648 | 0.826289 | 2.94E-54 | -1.69E-46 |
| MS.gene058084 | MS.gene40648 | 0.844105 | 9.29E-59 | -1.69E-46 |
| MS.gene058216 | MS.gene40648 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene40648 | 0.865961 | 4.04E-65 | -1.69E-46 |
| MS.gene058906 | MS.gene40648 | 0.883664 | 3.70E-71 | -1.69E-46 |
| MS.gene058911 | MS.gene40648 | 0.804067 | 2.58E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene40648 | 0.819709 | 1.01E-52 | -1.69E-46 |
| MS.gene05953 | MS.gene40648 | 0.801381 | 9.25E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene40648 | 0.820814 | 5.62E-53 | -1.69E-46 |
| MS.gene059830 | MS.gene40648 | 0.826394 | 2.78E-54 | -1.69E-46 |
| MS.gene059898 | MS.gene40648 | 0.832244 | 1.06E-55 | -1.69E-46 |
| MS.gene06001 | MS.gene40648 | 0.920417 | 1.31E-87 | -1.69E-46 |
| MS.gene06011 | MS.gene40648 | 0.824252 | 8.93E-54 | -1.69E-46 |
| MS.gene060429 | MS.gene40648 | 0.869336 | 3.34E-66 | -1.69E-46 |
| MS.gene060586 | MS.gene40648 | 0.843267 | 1.56E-58 | -1.69E-46 |
| MS.gene060830 | MS.gene40648 | 0.813322 | 2.71E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene40648 | 0.819359 | 1.21E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40648.t1 | MTR_4g068710 | 98.148 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 4.71e-35 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40648.t1 | AT1G49410 | 61.111 | 54 | 21 | 0 | 1 | 54 | 1 | 54 | 2.64e-23 | 82.8 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCTCATGTCGCCATGTT+TGG | 0.332693 | 4.1:+33696507 | MS.gene40648:CDS |
| TTCAAAGCCACAGCCTTGTC+TGG | 0.381218 | 4.1:-33696477 | None:intergenic |
| TTGAGAAAATGAAGAATGTA+AGG | 0.435892 | 4.1:-33696558 | None:intergenic |
| AAGGTAGCGATCATGTTTCC+AGG | 0.437874 | 4.1:+33696438 | None:intergenic |
| ATGTCGCCATGTTTGGAACA+TGG | 0.492135 | 4.1:+33696514 | MS.gene40648:CDS |
| TGTCGCCATGTTTGGAACAT+GGG | 0.532708 | 4.1:+33696515 | MS.gene40648:CDS |
| GCTTGAGTTCCTCGTTCTCG+CGG | 0.558292 | 4.1:-33696581 | None:intergenic |
| TGAGAAAATGAAGAATGTAA+GGG | 0.583076 | 4.1:-33696557 | None:intergenic |
| GAGAAAATGAAGAATGTAAG+GGG | 0.607716 | 4.1:-33696556 | None:intergenic |
| GCGAAAACCAGACAAGGCTG+TGG | 0.631734 | 4.1:+33696470 | MS.gene40648:CDS |
| AAGAACCCATGTTCCAAACA+TGG | 0.637331 | 4.1:-33696520 | None:intergenic |
| GTTCATGCGAAAACCAGACA+AGG | 0.672633 | 4.1:+33696464 | MS.gene40648:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAGAAAATGAAGAATGTAA+GGG | - | chr4.1:33696560-33696579 | None:intergenic | 25.0% |
| ! | TTGAGAAAATGAAGAATGTA+AGG | - | chr4.1:33696561-33696580 | None:intergenic | 25.0% |
| GAGAAAATGAAGAATGTAAG+GGG | - | chr4.1:33696559-33696578 | None:intergenic | 30.0% | |
| AAATCTCATGTCGCCATGTT+TGG | + | chr4.1:33696507-33696526 | MS.gene40648:CDS | 40.0% | |
| AAGAACCCATGTTCCAAACA+TGG | - | chr4.1:33696523-33696542 | None:intergenic | 40.0% | |
| GTTCATGCGAAAACCAGACA+AGG | + | chr4.1:33696464-33696483 | MS.gene40648:CDS | 45.0% | |
| ! | ATGTCGCCATGTTTGGAACA+TGG | + | chr4.1:33696514-33696533 | MS.gene40648:CDS | 45.0% |
| ! | GGTTTTCGCATGAACATTCC+TGG | - | chr4.1:33696459-33696478 | None:intergenic | 45.0% |
| ! | TGTCGCCATGTTTGGAACAT+GGG | + | chr4.1:33696515-33696534 | MS.gene40648:CDS | 45.0% |
| ! | TTCAAAGCCACAGCCTTGTC+TGG | - | chr4.1:33696480-33696499 | None:intergenic | 50.0% |
| ! | TTTTCTCAACCGCGAGAACG+AGG | + | chr4.1:33696572-33696591 | MS.gene40648:CDS | 50.0% |
| GCGAAAACCAGACAAGGCTG+TGG | + | chr4.1:33696470-33696489 | MS.gene40648:CDS | 55.0% | |
| GCTTGAGTTCCTCGTTCTCG+CGG | - | chr4.1:33696584-33696603 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 33696450 | 33696614 | 33696450 | ID=MS.gene40648 |
| chr4.1 | mRNA | 33696450 | 33696614 | 33696450 | ID=MS.gene40648.t1;Parent=MS.gene40648 |
| chr4.1 | exon | 33696450 | 33696614 | 33696450 | ID=MS.gene40648.t1.exon1;Parent=MS.gene40648.t1 |
| chr4.1 | CDS | 33696450 | 33696614 | 33696450 | ID=cds.MS.gene40648.t1;Parent=MS.gene40648.t1 |
| Gene Sequence |
| Protein sequence |
