Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48573.t1 | XP_013447599.1 | 96.9 | 351 | 11 | 0 | 1 | 351 | 1 | 351 | 2.50E-172 | 614.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48573.t1 | Q9S7W5 | 63.7 | 124 | 42 | 1 | 6 | 129 | 26 | 146 | 5.6e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48573.t1 | A0A072U750 | 96.9 | 351 | 11 | 0 | 1 | 351 | 1 | 351 | 1.8e-172 | 614.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene48573.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene48573 | 0.822041 | 2.93E-53 | -1.69E-46 |
| MS.gene049284 | MS.gene48573 | 0.816544 | 5.24E-52 | -1.69E-46 |
| MS.gene049356 | MS.gene48573 | 0.8185 | 1.90E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene48573 | 0.810169 | 1.32E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene48573 | 0.821083 | 4.88E-53 | -1.69E-46 |
| MS.gene049895 | MS.gene48573 | 0.802132 | 6.49E-49 | -1.69E-46 |
| MS.gene049910 | MS.gene48573 | 0.80044 | 1.44E-48 | -1.69E-46 |
| MS.gene050060 | MS.gene48573 | 0.826561 | 2.54E-54 | -1.69E-46 |
| MS.gene050128 | MS.gene48573 | 0.841322 | 5.10E-58 | -1.69E-46 |
| MS.gene050301 | MS.gene48573 | 0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene48573 | 0.858946 | 5.81E-63 | -1.69E-46 |
| MS.gene050400 | MS.gene48573 | 0.83092 | 2.24E-55 | -1.69E-46 |
| MS.gene050404 | MS.gene48573 | 0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene48573 | 0.827649 | 1.39E-54 | -1.69E-46 |
| MS.gene050426 | MS.gene48573 | -0.856491 | 3.10E-62 | -1.69E-46 |
| MS.gene050464 | MS.gene48573 | 0.861916 | 7.33E-64 | -1.69E-46 |
| MS.gene050529 | MS.gene48573 | 0.865718 | 4.82E-65 | -1.69E-46 |
| MS.gene050642 | MS.gene48573 | 0.815205 | 1.04E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene48573 | 0.839169 | 1.86E-57 | -1.69E-46 |
| MS.gene050971 | MS.gene48573 | 0.920786 | 8.23E-88 | -1.69E-46 |
| MS.gene05105 | MS.gene48573 | 0.819526 | 1.11E-52 | -1.69E-46 |
| MS.gene051525 | MS.gene48573 | 0.805738 | 1.15E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene48573 | 0.822145 | 2.77E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene48573 | 0.834632 | 2.68E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene48573 | -0.820707 | 5.95E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene48573 | 0.884888 | 1.30E-71 | -1.69E-46 |
| MS.gene052078 | MS.gene48573 | 0.803493 | 3.40E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene48573 | 0.838403 | 2.94E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene48573 | 0.86933 | 3.36E-66 | -1.69E-46 |
| MS.gene052253 | MS.gene48573 | 0.834887 | 2.31E-56 | -1.69E-46 |
| MS.gene052310 | MS.gene48573 | 0.847882 | 8.73E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene48573 | 0.880102 | 7.23E-70 | -1.69E-46 |
| MS.gene052469 | MS.gene48573 | 0.810676 | 1.02E-50 | -1.69E-46 |
| MS.gene052702 | MS.gene48573 | -0.80833 | 3.27E-50 | -1.69E-46 |
| MS.gene053073 | MS.gene48573 | 0.807655 | 4.55E-50 | -1.69E-46 |
| MS.gene05316 | MS.gene48573 | 0.867263 | 1.56E-65 | -1.69E-46 |
| MS.gene053449 | MS.gene48573 | 0.838573 | 2.65E-57 | -1.69E-46 |
| MS.gene053557 | MS.gene48573 | 0.870771 | 1.13E-66 | -1.69E-46 |
| MS.gene053758 | MS.gene48573 | 0.851638 | 7.79E-61 | -1.69E-46 |
| MS.gene053836 | MS.gene48573 | 0.819884 | 9.19E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene48573 | 0.814969 | 1.18E-51 | -1.69E-46 |
| MS.gene053928 | MS.gene48573 | 0.830563 | 2.74E-55 | -1.69E-46 |
| MS.gene054123 | MS.gene48573 | 0.811137 | 8.14E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene48573 | 0.87534 | 3.33E-68 | -1.69E-46 |
| MS.gene054955 | MS.gene48573 | 0.827337 | 1.65E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene48573 | 0.845476 | 3.97E-59 | -1.69E-46 |
| MS.gene055277 | MS.gene48573 | 0.825967 | 3.51E-54 | -1.69E-46 |
| MS.gene055422 | MS.gene48573 | 0.820399 | 7.00E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene48573 | 0.837346 | 5.49E-57 | -1.69E-46 |
| MS.gene055604 | MS.gene48573 | 0.812075 | 5.09E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene48573 | 0.818601 | 1.80E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene48573 | 0.835312 | 1.81E-56 | -1.69E-46 |
| MS.gene056330 | MS.gene48573 | -0.810931 | 9.02E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene48573 | 0.809665 | 1.69E-50 | -1.69E-46 |
| MS.gene056447 | MS.gene48573 | 0.802048 | 6.75E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene48573 | 0.829488 | 5.01E-55 | -1.69E-46 |
| MS.gene056609 | MS.gene48573 | 0.81444 | 1.54E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene48573 | 0.817623 | 3.00E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene48573 | 0.844869 | 5.79E-59 | -1.69E-46 |
| MS.gene056691 | MS.gene48573 | -0.801627 | 8.24E-49 | -1.69E-46 |
| MS.gene056719 | MS.gene48573 | 0.812299 | 4.55E-51 | -1.69E-46 |
| MS.gene056899 | MS.gene48573 | -0.83072 | 2.50E-55 | -1.69E-46 |
| MS.gene056979 | MS.gene48573 | 0.834459 | 2.96E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene48573 | 0.838628 | 2.57E-57 | -1.69E-46 |
| MS.gene057396 | MS.gene48573 | 0.860116 | 2.59E-63 | -1.69E-46 |
| MS.gene057448 | MS.gene48573 | 0.806178 | 9.33E-50 | -1.69E-46 |
| MS.gene057449 | MS.gene48573 | 0.802811 | 4.70E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene48573 | 0.811489 | 6.82E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene48573 | 0.808808 | 2.58E-50 | -1.69E-46 |
| MS.gene057779 | MS.gene48573 | 0.801589 | 8.38E-49 | -1.69E-46 |
| MS.gene057796 | MS.gene48573 | 0.832528 | 8.98E-56 | -1.69E-46 |
| MS.gene057799 | MS.gene48573 | 0.802129 | 6.49E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene48573 | 0.813618 | 2.34E-51 | -1.69E-46 |
| MS.gene057927 | MS.gene48573 | 0.835642 | 1.49E-56 | -1.69E-46 |
| MS.gene058007 | MS.gene48573 | 0.832724 | 8.03E-56 | -1.69E-46 |
| MS.gene058082 | MS.gene48573 | 0.812445 | 4.22E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene48573 | 0.82178 | 3.37E-53 | -1.69E-46 |
| MS.gene058142 | MS.gene48573 | 0.808244 | 3.41E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene48573 | 0.817483 | 3.22E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene48573 | 0.802919 | 4.46E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene48573 | 0.885716 | 6.40E-72 | -1.69E-46 |
| MS.gene05907 | MS.gene48573 | 0.846711 | 1.83E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene48573 | 0.842826 | 2.04E-58 | -1.69E-46 |
| MS.gene059294 | MS.gene48573 | 0.886102 | 4.58E-72 | -1.69E-46 |
| MS.gene059366 | MS.gene48573 | 0.817125 | 3.88E-52 | -1.69E-46 |
| MS.gene059402 | MS.gene48573 | -0.819888 | 9.17E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene48573 | 0.823789 | 1.15E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene48573 | 0.81166 | 6.26E-51 | -1.69E-46 |
| MS.gene05959 | MS.gene48573 | 0.815696 | 8.10E-52 | -1.69E-46 |
| MS.gene059674 | MS.gene48573 | 0.81461 | 1.41E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene48573 | 0.813357 | 2.67E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene48573 | 0.831611 | 1.51E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene48573 | 0.828965 | 6.70E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene48573 | 0.821919 | 3.13E-53 | -1.69E-46 |
| MS.gene059807 | MS.gene48573 | 0.874505 | 6.41E-68 | -1.69E-46 |
| MS.gene05984 | MS.gene48573 | 0.851262 | 9.95E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene48573 | 0.852476 | 4.50E-61 | -1.69E-46 |
| MS.gene060247 | MS.gene48573 | 0.808194 | 3.49E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene48573 | 0.810866 | 9.31E-51 | -1.69E-46 |
| MS.gene060495 | MS.gene48573 | 0.81511 | 1.09E-51 | -1.69E-46 |
| MS.gene060496 | MS.gene48573 | 0.818757 | 1.66E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene48573 | 0.808531 | 2.96E-50 | -1.69E-46 |
| MS.gene060986 | MS.gene48573 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene48573 | 0.819788 | 9.66E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene48573 | 0.864813 | 9.28E-65 | -1.69E-46 |
| MS.gene061051 | MS.gene48573 | -0.824003 | 1.02E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene48573 | 0.854334 | 1.32E-61 | -1.69E-46 |
| MS.gene061159 | MS.gene48573 | 0.848774 | 4.95E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48573.t1 | MTR_7g015010 | 97.151 | 351 | 10 | 0 | 1 | 351 | 1 | 351 | 0.0 | 696 |
| MS.gene48573.t1 | MTR_6g015350 | 42.973 | 370 | 154 | 15 | 1 | 351 | 2 | 333 | 1.43e-62 | 203 |
| MS.gene48573.t1 | MTR_4g109660 | 62.185 | 119 | 42 | 2 | 1 | 118 | 1 | 117 | 2.88e-45 | 157 |
| MS.gene48573.t1 | MTR_3g026050 | 44.783 | 230 | 101 | 9 | 1 | 209 | 1 | 225 | 2.88e-44 | 156 |
| MS.gene48573.t1 | MTR_8g463380 | 60.526 | 76 | 30 | 0 | 51 | 126 | 48 | 123 | 2.42e-24 | 103 |
| MS.gene48573.t1 | MTR_4g079580 | 58.442 | 77 | 32 | 0 | 51 | 127 | 16 | 92 | 6.67e-24 | 100 |
| MS.gene48573.t1 | MTR_2g078200 | 61.644 | 73 | 28 | 0 | 51 | 123 | 14 | 86 | 1.01e-23 | 99.8 |
| MS.gene48573.t1 | MTR_2g090960 | 60.811 | 74 | 29 | 0 | 51 | 124 | 18 | 91 | 3.39e-23 | 98.6 |
| MS.gene48573.t1 | MTR_4g111935 | 29.856 | 278 | 155 | 11 | 18 | 264 | 90 | 358 | 5.82e-16 | 78.6 |
| MS.gene48573.t1 | MTR_6g017055 | 39.623 | 106 | 58 | 3 | 58 | 158 | 127 | 231 | 8.17e-14 | 72.4 |
| MS.gene48573.t1 | MTR_7g018500 | 50.877 | 57 | 28 | 0 | 59 | 115 | 122 | 178 | 4.61e-13 | 70.1 |
| MS.gene48573.t1 | MTR_1g103380 | 48.333 | 60 | 31 | 0 | 56 | 115 | 200 | 259 | 1.00e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48573.t1 | AT3G02150 | 75.000 | 100 | 25 | 0 | 30 | 129 | 47 | 146 | 1.73e-45 | 157 |
| MS.gene48573.t1 | AT3G02150 | 75.000 | 100 | 25 | 0 | 30 | 129 | 47 | 146 | 4.60e-45 | 158 |
| MS.gene48573.t1 | AT5G08070 | 71.717 | 99 | 24 | 2 | 25 | 123 | 4 | 98 | 3.20e-42 | 147 |
| MS.gene48573.t1 | AT5G08070 | 71.717 | 99 | 24 | 2 | 25 | 123 | 4 | 98 | 3.20e-42 | 147 |
| MS.gene48573.t1 | AT5G60970 | 83.750 | 80 | 12 | 1 | 43 | 122 | 49 | 127 | 6.27e-42 | 150 |
| MS.gene48573.t1 | AT1G30210 | 75.000 | 72 | 18 | 0 | 47 | 118 | 40 | 111 | 9.39e-30 | 116 |
| MS.gene48573.t1 | AT1G30210 | 75.000 | 72 | 18 | 0 | 47 | 118 | 40 | 111 | 9.39e-30 | 116 |
| MS.gene48573.t1 | AT4G18390 | 35.000 | 260 | 112 | 8 | 47 | 298 | 32 | 242 | 3.59e-27 | 110 |
| MS.gene48573.t1 | AT4G18390 | 35.000 | 260 | 112 | 8 | 47 | 298 | 32 | 242 | 3.59e-27 | 110 |
| MS.gene48573.t1 | AT1G53230 | 61.842 | 76 | 29 | 0 | 51 | 126 | 43 | 118 | 2.02e-25 | 105 |
| MS.gene48573.t1 | AT1G53230 | 61.842 | 76 | 29 | 0 | 51 | 126 | 43 | 118 | 2.02e-25 | 105 |
| MS.gene48573.t1 | AT3G15030 | 60.811 | 74 | 29 | 0 | 51 | 124 | 39 | 112 | 1.11e-23 | 101 |
| MS.gene48573.t1 | AT3G15030 | 60.811 | 74 | 29 | 0 | 51 | 124 | 39 | 112 | 1.11e-23 | 101 |
| MS.gene48573.t1 | AT3G15030 | 60.811 | 74 | 29 | 0 | 51 | 124 | 39 | 112 | 1.11e-23 | 101 |
| MS.gene48573.t1 | AT3G15030 | 60.811 | 74 | 29 | 0 | 51 | 124 | 39 | 112 | 1.11e-23 | 101 |
| MS.gene48573.t1 | AT2G31070 | 64.615 | 65 | 23 | 0 | 51 | 115 | 23 | 87 | 5.44e-21 | 93.2 |
| MS.gene48573.t1 | AT1G68800 | 47.761 | 67 | 35 | 0 | 49 | 115 | 104 | 170 | 5.59e-15 | 75.5 |
| MS.gene48573.t1 | AT1G68800 | 47.761 | 67 | 35 | 0 | 49 | 115 | 104 | 170 | 5.92e-15 | 75.5 |
| MS.gene48573.t1 | AT1G67260 | 42.105 | 76 | 44 | 0 | 46 | 121 | 76 | 151 | 6.70e-15 | 75.5 |
| MS.gene48573.t1 | AT1G67260 | 42.105 | 76 | 44 | 0 | 46 | 121 | 76 | 151 | 6.70e-15 | 75.5 |
| MS.gene48573.t1 | AT1G67260 | 42.105 | 76 | 44 | 0 | 46 | 121 | 76 | 151 | 6.94e-15 | 75.5 |
| MS.gene48573.t1 | AT3G18550 | 44.048 | 84 | 41 | 2 | 32 | 115 | 129 | 206 | 1.22e-13 | 72.0 |
| MS.gene48573.t1 | AT3G18550 | 44.048 | 84 | 41 | 2 | 32 | 115 | 129 | 206 | 1.33e-13 | 72.0 |
| MS.gene48573.t1 | AT3G18550 | 44.048 | 84 | 41 | 2 | 32 | 115 | 129 | 206 | 1.33e-13 | 72.0 |
| MS.gene48573.t1 | AT3G18550 | 44.048 | 84 | 41 | 2 | 32 | 115 | 129 | 206 | 1.56e-13 | 71.6 |
Find 61 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATCACCCTTCATTTAT+TGG | 0.099357 | 7.2:-82903441 | MS.gene48573:CDS |
| TGTAACATATTGTTATTATT+TGG | 0.154767 | 7.2:+82903468 | None:intergenic |
| CTTCAAGTCAAAATTCTTCT+TGG | 0.156785 | 7.2:-82903623 | MS.gene48573:CDS |
| AGTGAACTCTTGTTCTTTAT+TGG | 0.160335 | 7.2:+82903068 | None:intergenic |
| AATTCTCTTGAAACTTCTTT+AGG | 0.204373 | 7.2:+82903591 | None:intergenic |
| GTTCCTACAGGTGGAAAATT+AGG | 0.209351 | 7.2:+82903738 | None:intergenic |
| ATACTATTCATCAAGTAATT+TGG | 0.215929 | 7.2:+82903555 | None:intergenic |
| CTTGATACTCTGACAATTCT+TGG | 0.239929 | 7.2:+82903957 | None:intergenic |
| TGCTGAAAATTCTTCTCAAT+TGG | 0.271973 | 7.2:-82903361 | MS.gene48573:CDS |
| ATTCTCTTGAAACTTCTTTA+GGG | 0.288069 | 7.2:+82903592 | None:intergenic |
| CCAATAAATGAAGGGTGATT+TGG | 0.289695 | 7.2:+82903441 | None:intergenic |
| ATTCTTGGATCTTTCAATCT+TGG | 0.296306 | 7.2:+82903972 | None:intergenic |
| GTCAGAGTATCAAGAGCTTT+TGG | 0.318336 | 7.2:-82903948 | MS.gene48573:CDS |
| TAAAGAAGTTTCAAGAGAAT+TGG | 0.320601 | 7.2:-82903589 | MS.gene48573:CDS |
| AATTCTTCTCAAATGATGTT+AGG | 0.334825 | 7.2:-82903285 | MS.gene48573:CDS |
| TCAATAGGAACAATATTCAT+TGG | 0.339202 | 7.2:-82903656 | MS.gene48573:CDS |
| TATGATCTTCAAGATAGGTT+AGG | 0.344793 | 7.2:-82903837 | MS.gene48573:CDS |
| GATTGAGAGATAGAAGAATA+AGG | 0.365377 | 7.2:-82903887 | MS.gene48573:CDS |
| CCAATGTTGTCTTTATCAAC+TGG | 0.374206 | 7.2:-82903309 | MS.gene48573:CDS |
| TGATGAACCTCTTGTTTCAA+TGG | 0.375029 | 7.2:+82904065 | None:intergenic |
| AACAATTGTTCAATTTCAAT+AGG | 0.377158 | 7.2:-82903671 | MS.gene48573:CDS |
| AACCTAGCAAAGTTGTTGAT+TGG | 0.387978 | 7.2:-82903806 | MS.gene48573:CDS |
| GCTGAAAATTCTTCTCAATT+GGG | 0.390841 | 7.2:-82903360 | MS.gene48573:CDS |
| CAACAATCCATCCTTCAGTT+TGG | 0.407175 | 7.2:-82903124 | MS.gene48573:CDS |
| GTAGGAACAAATTTCAACTT+TGG | 0.419771 | 7.2:-82903723 | MS.gene48573:CDS |
| AACTTTATGATCTTCAAGAT+AGG | 0.421843 | 7.2:-82903842 | MS.gene48573:CDS |
| ATGATCTTCAAGATAGGTTA+GGG | 0.433727 | 7.2:-82903836 | MS.gene48573:CDS |
| AGCAAAGTATGCACAATAAG+AGG | 0.437085 | 7.2:-82903909 | MS.gene48573:CDS |
| TTTAATAAACCAATAAATGA+AGG | 0.455410 | 7.2:+82903432 | None:intergenic |
| CAATAGGAACAATATTCATT+GGG | 0.458388 | 7.2:-82903655 | MS.gene48573:CDS |
| TTGAAATTTGTTCCTACAGG+TGG | 0.465228 | 7.2:+82903729 | None:intergenic |
| TTGTTGAGAGAATTAGTCAT+TGG | 0.470696 | 7.2:+82903141 | None:intergenic |
| AAGTTGAAATTTGTTCCTAC+AGG | 0.474911 | 7.2:+82903726 | None:intergenic |
| TTTGATGATAATGATGGTTG+TGG | 0.483862 | 7.2:+82903193 | None:intergenic |
| ACTTGGTACTAATTACCAAT+GGG | 0.492610 | 7.2:-82903400 | MS.gene48573:CDS |
| TTCTTCAAATGTTAATGCAA+TGG | 0.492790 | 7.2:-82903244 | MS.gene48573:CDS |
| TAGAAATTTGGCCAAACTGA+AGG | 0.496121 | 7.2:+82903113 | None:intergenic |
| TTGTTGAGTTCATTAGAAGA+TGG | 0.499012 | 7.2:+82903696 | None:intergenic |
| TCTTCTCAATTGGGAAACAA+TGG | 0.501500 | 7.2:-82903351 | MS.gene48573:CDS |
| TTATTAAATCCTATGCCACT+TGG | 0.521662 | 7.2:-82903417 | MS.gene48573:CDS |
| AAATGTTAATGCAATGGAGA+TGG | 0.523566 | 7.2:-82903238 | MS.gene48573:CDS |
| ACAGGTGGAAAATTAGGTAA+TGG | 0.536982 | 7.2:+82903744 | None:intergenic |
| CCAGTTGATAAAGACAACAT+TGG | 0.545201 | 7.2:+82903309 | None:intergenic |
| TCAACATTTGATGATAATGA+TGG | 0.545561 | 7.2:+82903187 | None:intergenic |
| AACCAATCAACAACTTTGCT+AGG | 0.547433 | 7.2:+82903804 | None:intergenic |
| TAATTAGTACCAAGTGGCAT+AGG | 0.547722 | 7.2:+82903408 | None:intergenic |
| GATGAAAATATTAACAAGCA+AGG | 0.549563 | 7.2:-82903525 | MS.gene48573:CDS |
| CACTTGGTACTAATTACCAA+TGG | 0.556420 | 7.2:-82903401 | MS.gene48573:CDS |
| CATTGGAAGTACCTCCCCAT+TGG | 0.558728 | 7.2:+82903385 | None:intergenic |
| AATTTGGCCAAACTGAAGGA+TGG | 0.562622 | 7.2:+82903117 | None:intergenic |
| TGGCCATTGTGATGAAGCTA+AGG | 0.563573 | 7.2:+82903992 | None:intergenic |
| AGGAACAATATTCATTGGGA+AGG | 0.563907 | 7.2:-82903651 | MS.gene48573:CDS |
| GGTGGAAAATTAGGTAATGG+TGG | 0.564196 | 7.2:+82903747 | None:intergenic |
| GTTGATAAAGACAACATTGG+TGG | 0.570845 | 7.2:+82903312 | None:intergenic |
| TCATAAAGTTGAATAGCTGT+TGG | 0.592296 | 7.2:+82903855 | None:intergenic |
| TTAATAAACCAATAAATGAA+GGG | 0.612685 | 7.2:+82903433 | None:intergenic |
| GAAGCTAAGGAGCTTGATGA+TGG | 0.616225 | 7.2:+82904005 | None:intergenic |
| GCTCCTTAGCTTCATCACAA+TGG | 0.647279 | 7.2:-82903995 | MS.gene48573:CDS |
| GGTACTAATTACCAATGGGG+AGG | 0.679474 | 7.2:-82903396 | MS.gene48573:CDS |
| CATTGGTAATTAGTACCAAG+TGG | 0.701178 | 7.2:+82903402 | None:intergenic |
| CTTGGTACTAATTACCAATG+GGG | 0.737610 | 7.2:-82903399 | MS.gene48573:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATAAACCAATAAATGAA+GGG | + | chr7.2:82903721-82903740 | None:intergenic | 15.0% |
| !! | TTTAATAAACCAATAAATGA+AGG | + | chr7.2:82903722-82903741 | None:intergenic | 15.0% |
| !!! | TGTAACATATTGTTATTATT+TGG | + | chr7.2:82903686-82903705 | None:intergenic | 15.0% |
| !! | AACAATTGTTCAATTTCAAT+AGG | - | chr7.2:82903480-82903499 | MS.gene48573:CDS | 20.0% |
| !! | ATACTATTCATCAAGTAATT+TGG | + | chr7.2:82903599-82903618 | None:intergenic | 20.0% |
| ! | AAACGATCATGAAAAAGAAA+AGG | - | chr7.2:82903115-82903134 | MS.gene48573:CDS | 25.0% |
| ! | AACTTTATGATCTTCAAGAT+AGG | - | chr7.2:82903309-82903328 | MS.gene48573:CDS | 25.0% |
| ! | AATTCTCTTGAAACTTCTTT+AGG | + | chr7.2:82903563-82903582 | None:intergenic | 25.0% |
| ! | AATTCTTCTCAAATGATGTT+AGG | - | chr7.2:82903866-82903885 | MS.gene48573:CDS | 25.0% |
| ! | ATTCTCTTGAAACTTCTTTA+GGG | + | chr7.2:82903562-82903581 | None:intergenic | 25.0% |
| ! | CAATAGGAACAATATTCATT+GGG | - | chr7.2:82903496-82903515 | MS.gene48573:CDS | 25.0% |
| ! | GATGAAAATATTAACAAGCA+AGG | - | chr7.2:82903626-82903645 | MS.gene48573:CDS | 25.0% |
| ! | TAAAGAAGTTTCAAGAGAAT+TGG | - | chr7.2:82903562-82903581 | MS.gene48573:CDS | 25.0% |
| ! | TCAATAGGAACAATATTCAT+TGG | - | chr7.2:82903495-82903514 | MS.gene48573:CDS | 25.0% |
| ! | TTCTTCAAATGTTAATGCAA+TGG | - | chr7.2:82903907-82903926 | MS.gene48573:CDS | 25.0% |
| !! | TCAACATTTGATGATAATGA+TGG | + | chr7.2:82903967-82903986 | None:intergenic | 25.0% |
| AAATGTTAATGCAATGGAGA+TGG | - | chr7.2:82903913-82903932 | MS.gene48573:CDS | 30.0% | |
| ACTTGGTACTAATTACCAAT+GGG | - | chr7.2:82903751-82903770 | MS.gene48573:CDS | 30.0% | |
| AGTGAACTCTTGTTCTTTAT+TGG | + | chr7.2:82904086-82904105 | None:intergenic | 30.0% | |
| ATGATCTTCAAGATAGGTTA+GGG | - | chr7.2:82903315-82903334 | MS.gene48573:CDS | 30.0% | |
| ATTCTTGGATCTTTCAATCT+TGG | + | chr7.2:82903182-82903201 | None:intergenic | 30.0% | |
| CTTCAAGTCAAAATTCTTCT+TGG | - | chr7.2:82903528-82903547 | MS.gene48573:CDS | 30.0% | |
| GATTGAGAGATAGAAGAATA+AGG | - | chr7.2:82903264-82903283 | MS.gene48573:CDS | 30.0% | |
| GCTGAAAATTCTTCTCAATT+GGG | - | chr7.2:82903791-82903810 | MS.gene48573:CDS | 30.0% | |
| GTAGGAACAAATTTCAACTT+TGG | - | chr7.2:82903428-82903447 | MS.gene48573:CDS | 30.0% | |
| TATGATCTTCAAGATAGGTT+AGG | - | chr7.2:82903314-82903333 | MS.gene48573:CDS | 30.0% | |
| TCATAAAGTTGAATAGCTGT+TGG | + | chr7.2:82903299-82903318 | None:intergenic | 30.0% | |
| TGCTGAAAATTCTTCTCAAT+TGG | - | chr7.2:82903790-82903809 | MS.gene48573:CDS | 30.0% | |
| TTATTAAATCCTATGCCACT+TGG | - | chr7.2:82903734-82903753 | MS.gene48573:CDS | 30.0% | |
| TTGTTGAGAGAATTAGTCAT+TGG | + | chr7.2:82904013-82904032 | None:intergenic | 30.0% | |
| TTGTTGAGTTCATTAGAAGA+TGG | + | chr7.2:82903458-82903477 | None:intergenic | 30.0% | |
| TTTGGAAAAACATTTGCAGT+AGG | + | chr7.2:82903668-82903687 | None:intergenic | 30.0% | |
| ! | AAGTTGAAATTTGTTCCTAC+AGG | + | chr7.2:82903428-82903447 | None:intergenic | 30.0% |
| ! | AGAAGAATTTTCAGCATCAT+TGG | + | chr7.2:82903786-82903805 | None:intergenic | 30.0% |
| ! | AGATTTTCCATTGAAACAAG+AGG | - | chr7.2:82903079-82903098 | MS.gene48573:CDS | 30.0% |
| ! | TTTGATGATAATGATGGTTG+TGG | + | chr7.2:82903961-82903980 | None:intergenic | 30.0% |
| !!! | TCTGCTGTTTTGTAGAAATT+TGG | + | chr7.2:82904053-82904072 | None:intergenic | 30.0% |
| AACCAATCAACAACTTTGCT+AGG | + | chr7.2:82903350-82903369 | None:intergenic | 35.0% | |
| AACCTAGCAAAGTTGTTGAT+TGG | - | chr7.2:82903345-82903364 | MS.gene48573:CDS | 35.0% | |
| ACAGGTGGAAAATTAGGTAA+TGG | + | chr7.2:82903410-82903429 | None:intergenic | 35.0% | |
| AGCAAAGTATGCACAATAAG+AGG | - | chr7.2:82903242-82903261 | MS.gene48573:CDS | 35.0% | |
| AGGAACAATATTCATTGGGA+AGG | - | chr7.2:82903500-82903519 | MS.gene48573:CDS | 35.0% | |
| CACTTGGTACTAATTACCAA+TGG | - | chr7.2:82903750-82903769 | MS.gene48573:CDS | 35.0% | |
| CCAAATCACCCTTCATTTAT+TGG | - | chr7.2:82903710-82903729 | MS.gene48573:CDS | 35.0% | |
| CCAATAAATGAAGGGTGATT+TGG | + | chr7.2:82903713-82903732 | None:intergenic | 35.0% | |
| CCAATGTTGTCTTTATCAAC+TGG | - | chr7.2:82903842-82903861 | MS.gene48573:CDS | 35.0% | |
| CTTGATACTCTGACAATTCT+TGG | + | chr7.2:82903197-82903216 | None:intergenic | 35.0% | |
| CTTGGTACTAATTACCAATG+GGG | - | chr7.2:82903752-82903771 | MS.gene48573:CDS | 35.0% | |
| TAATTAGTACCAAGTGGCAT+AGG | + | chr7.2:82903746-82903765 | None:intergenic | 35.0% | |
| TAGAAATTTGGCCAAACTGA+AGG | + | chr7.2:82904041-82904060 | None:intergenic | 35.0% | |
| TCTTCTCAATTGGGAAACAA+TGG | - | chr7.2:82903800-82903819 | MS.gene48573:CDS | 35.0% | |
| ! | CATTGGTAATTAGTACCAAG+TGG | + | chr7.2:82903752-82903771 | None:intergenic | 35.0% |
| ! | GTTGATAAAGACAACATTGG+TGG | + | chr7.2:82903842-82903861 | None:intergenic | 35.0% |
| ! | TGATGAACCTCTTGTTTCAA+TGG | + | chr7.2:82903089-82903108 | None:intergenic | 35.0% |
| ! | TTACCTAATTTTCCACCTGT+AGG | - | chr7.2:82903410-82903429 | MS.gene48573:CDS | 35.0% |
| ! | TTGAAATTTGTTCCTACAGG+TGG | + | chr7.2:82903425-82903444 | None:intergenic | 35.0% |
| !! | CCAGTTGATAAAGACAACAT+TGG | + | chr7.2:82903845-82903864 | None:intergenic | 35.0% |
| AATTTGGCCAAACTGAAGGA+TGG | + | chr7.2:82904037-82904056 | None:intergenic | 40.0% | |
| CAACAATCCATCCTTCAGTT+TGG | - | chr7.2:82904027-82904046 | MS.gene48573:CDS | 40.0% | |
| GTTCCTACAGGTGGAAAATT+AGG | + | chr7.2:82903416-82903435 | None:intergenic | 40.0% | |
| ! | GGTGGAAAATTAGGTAATGG+TGG | + | chr7.2:82903407-82903426 | None:intergenic | 40.0% |
| !! | AGAGTATCAAGAGCTTTTGG+TGG | - | chr7.2:82903206-82903225 | MS.gene48573:CDS | 40.0% |
| !! | GTCAGAGTATCAAGAGCTTT+TGG | - | chr7.2:82903203-82903222 | MS.gene48573:CDS | 40.0% |
| GAAGCTAAGGAGCTTGATGA+TGG | + | chr7.2:82903149-82903168 | None:intergenic | 45.0% | |
| GCTCCTTAGCTTCATCACAA+TGG | - | chr7.2:82903156-82903175 | MS.gene48573:CDS | 45.0% | |
| GGTACTAATTACCAATGGGG+AGG | - | chr7.2:82903755-82903774 | MS.gene48573:CDS | 45.0% | |
| TGGCCATTGTGATGAAGCTA+AGG | + | chr7.2:82903162-82903181 | None:intergenic | 45.0% | |
| !! | CATTGGAAGTACCTCCCCAT+TGG | + | chr7.2:82903769-82903788 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 82903059 | 82904114 | 82903059 | ID=MS.gene48573 |
| chr7.2 | mRNA | 82903059 | 82904114 | 82903059 | ID=MS.gene48573.t1;Parent=MS.gene48573 |
| chr7.2 | exon | 82903059 | 82904114 | 82903059 | ID=MS.gene48573.t1.exon1;Parent=MS.gene48573.t1 |
| chr7.2 | CDS | 82903059 | 82904114 | 82903059 | ID=cds.MS.gene48573.t1;Parent=MS.gene48573.t1 |
| Gene Sequence |
| Protein sequence |
