Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69736.t1 | XP_003601450.1 | 91.7 | 133 | 8 | 2 | 1 | 132 | 1 | 131 | 1.20E-59 | 239.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69736.t1 | G7J804 | 91.7 | 133 | 8 | 2 | 1 | 132 | 1 | 131 | 9.0e-60 | 239.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene69736 | 0.831755 | 1.39E-55 | -1.69E-46 |
| MS.gene049217 | MS.gene69736 | 0.820176 | 7.88E-53 | -1.69E-46 |
| MS.gene049356 | MS.gene69736 | 0.852691 | 3.91E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene69736 | 0.850319 | 1.83E-60 | -1.69E-46 |
| MS.gene04938 | MS.gene69736 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene04939 | MS.gene69736 | 0.810775 | 9.75E-51 | -1.69E-46 |
| MS.gene049488 | MS.gene69736 | 0.821659 | 3.59E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene69736 | 0.827356 | 1.64E-54 | -1.69E-46 |
| MS.gene049805 | MS.gene69736 | 0.856592 | 2.90E-62 | -1.69E-46 |
| MS.gene050520 | MS.gene69736 | 0.803813 | 2.91E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene69736 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene69736 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene69736 | 0.837923 | 3.90E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene69736 | 0.851044 | 1.15E-60 | -1.69E-46 |
| MS.gene051387 | MS.gene69736 | 0.802008 | 6.88E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene69736 | 0.805038 | 1.62E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene69736 | 0.800508 | 1.39E-48 | -1.69E-46 |
| MS.gene051858 | MS.gene69736 | 0.819286 | 1.26E-52 | -1.69E-46 |
| MS.gene051874 | MS.gene69736 | 0.807689 | 4.47E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene69736 | 0.824802 | 6.63E-54 | -1.69E-46 |
| MS.gene052271 | MS.gene69736 | 0.838134 | 3.44E-57 | -1.69E-46 |
| MS.gene052310 | MS.gene69736 | 0.813853 | 2.07E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene69736 | 0.807936 | 3.96E-50 | -1.69E-46 |
| MS.gene052421 | MS.gene69736 | 0.870268 | 1.66E-66 | -1.69E-46 |
| MS.gene052484 | MS.gene69736 | 0.804084 | 2.56E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene69736 | 0.807318 | 5.36E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene69736 | 0.851705 | 7.46E-61 | -1.69E-46 |
| MS.gene052999 | MS.gene69736 | 0.807228 | 5.60E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene69736 | 0.808183 | 3.51E-50 | -1.69E-46 |
| MS.gene053213 | MS.gene69736 | 0.804619 | 1.98E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene69736 | 0.821633 | 3.64E-53 | -1.69E-46 |
| MS.gene053480 | MS.gene69736 | 0.805944 | 1.05E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene69736 | 0.806652 | 7.41E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene69736 | 0.848103 | 7.58E-60 | -1.69E-46 |
| MS.gene053758 | MS.gene69736 | 0.8298 | 4.20E-55 | -1.69E-46 |
| MS.gene053813 | MS.gene69736 | 0.836314 | 1.01E-56 | -1.69E-46 |
| MS.gene053912 | MS.gene69736 | 0.863303 | 2.74E-64 | -1.69E-46 |
| MS.gene053915 | MS.gene69736 | 0.820422 | 6.92E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene69736 | 0.838676 | 2.50E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene69736 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene69736 | 0.829089 | 6.26E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene69736 | 0.866158 | 3.50E-65 | -1.69E-46 |
| MS.gene055206 | MS.gene69736 | 0.806046 | 9.95E-50 | -1.69E-46 |
| MS.gene055397 | MS.gene69736 | 0.810657 | 1.03E-50 | -1.69E-46 |
| MS.gene055412 | MS.gene69736 | 0.812544 | 4.02E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene69736 | 0.810545 | 1.09E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene69736 | 0.828562 | 8.39E-55 | -1.69E-46 |
| MS.gene055741 | MS.gene69736 | 0.804881 | 1.75E-49 | -1.69E-46 |
| MS.gene055744 | MS.gene69736 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene055909 | MS.gene69736 | 0.824903 | 6.27E-54 | -1.69E-46 |
| MS.gene055911 | MS.gene69736 | 0.824487 | 7.86E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene69736 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene055941 | MS.gene69736 | 0.847543 | 1.08E-59 | -1.69E-46 |
| MS.gene056449 | MS.gene69736 | 0.826494 | 2.63E-54 | -1.69E-46 |
| MS.gene056487 | MS.gene69736 | 0.803307 | 3.71E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene69736 | 0.834294 | 3.26E-56 | -1.69E-46 |
| MS.gene056609 | MS.gene69736 | 0.843514 | 1.34E-58 | -1.69E-46 |
| MS.gene056657 | MS.gene69736 | 0.806039 | 9.98E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene69736 | 0.875225 | 3.64E-68 | -1.69E-46 |
| MS.gene057124 | MS.gene69736 | 0.815518 | 8.87E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene69736 | 0.807167 | 5.77E-50 | -1.69E-46 |
| MS.gene057146 | MS.gene69736 | 0.810255 | 1.26E-50 | -1.69E-46 |
| MS.gene05735 | MS.gene69736 | 0.803495 | 3.39E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene69736 | 0.825528 | 4.46E-54 | -1.69E-46 |
| MS.gene057734 | MS.gene69736 | 0.824866 | 6.40E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene69736 | 0.835711 | 1.43E-56 | -1.69E-46 |
| MS.gene057823 | MS.gene69736 | 0.852023 | 6.06E-61 | -1.69E-46 |
| MS.gene058019 | MS.gene69736 | 0.827711 | 1.35E-54 | -1.69E-46 |
| MS.gene058120 | MS.gene69736 | 0.816857 | 4.46E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene69736 | 0.807669 | 4.51E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene69736 | 0.801332 | 9.47E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene69736 | 0.810377 | 1.19E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene69736 | 0.847214 | 1.33E-59 | -1.69E-46 |
| MS.gene058948 | MS.gene69736 | 0.810607 | 1.06E-50 | -1.69E-46 |
| MS.gene059366 | MS.gene69736 | 0.826067 | 3.32E-54 | -1.69E-46 |
| MS.gene059367 | MS.gene69736 | 0.809312 | 2.01E-50 | -1.69E-46 |
| MS.gene059368 | MS.gene69736 | 0.834077 | 3.69E-56 | -1.69E-46 |
| MS.gene059455 | MS.gene69736 | 0.858655 | 7.10E-63 | -1.69E-46 |
| MS.gene059674 | MS.gene69736 | 0.843324 | 1.50E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene69736 | 0.8052 | 1.50E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene69736 | 0.829057 | 6.37E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene69736 | 0.829605 | 4.69E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene69736 | 0.831936 | 1.26E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene69736 | 0.854354 | 1.30E-61 | -1.69E-46 |
| MS.gene059718 | MS.gene69736 | 0.806338 | 8.64E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene69736 | 0.811732 | 6.04E-51 | -1.69E-46 |
| MS.gene060469 | MS.gene69736 | 0.802299 | 5.99E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene69736 | 0.808707 | 2.71E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene69736 | 0.834138 | 3.56E-56 | -1.69E-46 |
| MS.gene060835 | MS.gene69736 | 0.838528 | 2.73E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene69736 | 0.829858 | 4.07E-55 | -1.69E-46 |
| MS.gene060981 | MS.gene69736 | 0.829187 | 5.92E-55 | -1.69E-46 |
| MS.gene061041 | MS.gene69736 | 0.830603 | 2.68E-55 | -1.69E-46 |
| MS.gene061200 | MS.gene69736 | 0.827834 | 1.26E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69736.t1 | MTR_3g080840 | 91.729 | 133 | 8 | 2 | 1 | 132 | 1 | 131 | 4.79e-77 | 230 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69736.t1 | AT1G64680 | 64.762 | 105 | 30 | 1 | 28 | 132 | 18 | 115 | 3.83e-39 | 131 |
| MS.gene69736.t1 | AT1G64680 | 64.762 | 105 | 30 | 1 | 28 | 132 | 18 | 115 | 1.88e-38 | 131 |
Find 29 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGGTTGGTTGGATATT+TGG | 0.135539 | 3.4:-77004809 | MS.gene69736:CDS |
| TTTCCTCTTGGAGCATATTT+TGG | 0.212329 | 3.4:+77005011 | None:intergenic |
| GAAAGGTTGGTTGGATATTT+GGG | 0.230670 | 3.4:-77004808 | MS.gene69736:CDS |
| TGTTTAGCATTCCCTATTAC+AGG | 0.236171 | 3.4:+77004843 | None:intergenic |
| CCATTTGCAGAGCCTGTAAT+AGG | 0.265083 | 3.4:-77004855 | MS.gene69736:CDS |
| TCCTCCAGGTGCGCCTGCTC+AGG | 0.352565 | 3.4:-77004682 | MS.gene69736:CDS |
| TCTGGTGAACCTGCTCCTTT+AGG | 0.365720 | 3.4:-77004948 | MS.gene69736:CDS |
| AAATATGCTCCAAGAGGAAA+TGG | 0.435825 | 3.4:-77005008 | MS.gene69736:CDS |
| CCTATTACAGGCTCTGCAAA+TGG | 0.450834 | 3.4:+77004855 | None:intergenic |
| TCCACCTGAGCAGGCGCACC+TGG | 0.454996 | 3.4:+77004678 | None:intergenic |
| GCAGTAGCACCATTTCCTCT+TGG | 0.483001 | 3.4:+77004999 | None:intergenic |
| AAAAGAAGGAAAAGAAAGGT+TGG | 0.484991 | 3.4:-77004821 | MS.gene69736:CDS |
| TCCAGGTGCGCCTGCTCAGG+TGG | 0.485107 | 3.4:-77004679 | MS.gene69736:CDS |
| CATTTGCAGAGCCTGTAATA+GGG | 0.488770 | 3.4:-77004854 | MS.gene69736:CDS |
| CTTCTCTCTATGCTTCCTCC+AGG | 0.491765 | 3.4:-77004696 | MS.gene69736:CDS |
| GAAGGAAAAGAAAGGTTGGT+TGG | 0.501209 | 3.4:-77004817 | MS.gene69736:CDS |
| AGGGAATGCTAAACAAAAGA+AGG | 0.514346 | 3.4:-77004835 | MS.gene69736:CDS |
| TCTATAATATCCACCTGAGC+AGG | 0.524418 | 3.4:+77004669 | None:intergenic |
| TGTGGTATAGCTGAACCATC+TGG | 0.549247 | 3.4:-77004966 | MS.gene69736:CDS |
| AAACAAAAGAAGGAAAAGAA+AGG | 0.569359 | 3.4:-77004825 | MS.gene69736:CDS |
| AAAGGAGCAGGTTCACCAGA+TGG | 0.579959 | 3.4:+77004951 | None:intergenic |
| TTTGGTGGAGTTATTAGCTG+AGG | 0.595601 | 3.4:+77005029 | None:intergenic |
| ACCTGAGCAGGCGCACCTGG+AGG | 0.601220 | 3.4:+77004681 | None:intergenic |
| GCAACAGCAAGTTGTTCGTG+AGG | 0.628043 | 3.4:-77004721 | MS.gene69736:CDS |
| CGAAAAGAGTAATGTTGCGT+CGG | 0.630829 | 3.4:-77004755 | MS.gene69736:CDS |
| TTCAGCTATACCACAACGGA+TGG | 0.634641 | 3.4:+77004974 | None:intergenic |
| CCACCAAAATATGCTCCAAG+AGG | 0.652709 | 3.4:-77005014 | MS.gene69736:CDS |
| GCTACTGCTACCATCCGTTG+TGG | 0.697016 | 3.4:-77004984 | MS.gene69736:CDS |
| ATGGTTCAGCTATACCACAA+CGG | 0.757452 | 3.4:+77004970 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACAAAAGAAGGAAAAGAA+AGG | - | chr3.4:77004854-77004873 | MS.gene69736:CDS | 25.0% |
| AAAAGAAGGAAAAGAAAGGT+TGG | - | chr3.4:77004858-77004877 | MS.gene69736:CDS | 30.0% | |
| ! | GTATGATTATGAGAGTTTTG+TGG | - | chr3.4:77004895-77004914 | MS.gene69736:CDS | 30.0% |
| !! | CAATGACAGTATTTTTGAGA+AGG | - | chr3.4:77004769-77004788 | MS.gene69736:CDS | 30.0% |
| AAATATGCTCCAAGAGGAAA+TGG | - | chr3.4:77004671-77004690 | MS.gene69736:CDS | 35.0% | |
| AGGGAATGCTAAACAAAAGA+AGG | - | chr3.4:77004844-77004863 | MS.gene69736:CDS | 35.0% | |
| TGTTTAGCATTCCCTATTAC+AGG | + | chr3.4:77004839-77004858 | None:intergenic | 35.0% | |
| ! | AGAAAGGTTGGTTGGATATT+TGG | - | chr3.4:77004870-77004889 | MS.gene69736:CDS | 35.0% |
| ! | GAAAGGTTGGTTGGATATTT+GGG | - | chr3.4:77004871-77004890 | MS.gene69736:CDS | 35.0% |
| ! | GACTTTGTTTGCACGAAAAA+TGG | - | chr3.4:77004799-77004818 | MS.gene69736:CDS | 35.0% |
| ! | TAACGTGTCTTTTGTCCTAA+AGG | + | chr3.4:77004749-77004768 | None:intergenic | 35.0% |
| ! | TTTCCTCTTGGAGCATATTT+TGG | + | chr3.4:77004671-77004690 | None:intergenic | 35.0% |
| ATGGTTCAGCTATACCACAA+CGG | + | chr3.4:77004712-77004731 | None:intergenic | 40.0% | |
| CATTTGCAGAGCCTGTAATA+GGG | - | chr3.4:77004825-77004844 | MS.gene69736:CDS | 40.0% | |
| CGAAAAGAGTAATGTTGCGT+CGG | - | chr3.4:77004924-77004943 | MS.gene69736:CDS | 40.0% | |
| TCTATAATATCCACCTGAGC+AGG | + | chr3.4:77005013-77005032 | None:intergenic | 40.0% | |
| TTTGGTGGAGTTATTAGCTG+AGG | + | chr3.4:77004653-77004672 | None:intergenic | 40.0% | |
| ! | GAAGGAAAAGAAAGGTTGGT+TGG | - | chr3.4:77004862-77004881 | MS.gene69736:CDS | 40.0% |
| CCACCAAAATATGCTCCAAG+AGG | - | chr3.4:77004665-77004684 | MS.gene69736:CDS | 45.0% | |
| CCATTTGCAGAGCCTGTAAT+AGG | - | chr3.4:77004824-77004843 | MS.gene69736:CDS | 45.0% | |
| CCTATTACAGGCTCTGCAAA+TGG | + | chr3.4:77004827-77004846 | None:intergenic | 45.0% | |
| TGTGGTATAGCTGAACCATC+TGG | - | chr3.4:77004713-77004732 | MS.gene69736:CDS | 45.0% | |
| TTCAGCTATACCACAACGGA+TGG | + | chr3.4:77004708-77004727 | None:intergenic | 45.0% | |
| ! | GTCTTTTGTCCTAAAGGAGC+AGG | + | chr3.4:77004743-77004762 | None:intergenic | 45.0% |
| !! | CCTCTTGGAGCATATTTTGG+TGG | + | chr3.4:77004668-77004687 | None:intergenic | 45.0% |
| AAAGGAGCAGGTTCACCAGA+TGG | + | chr3.4:77004731-77004750 | None:intergenic | 50.0% | |
| CTTCTCTCTATGCTTCCTCC+AGG | - | chr3.4:77004983-77005002 | MS.gene69736:CDS | 50.0% | |
| GCAACAGCAAGTTGTTCGTG+AGG | - | chr3.4:77004958-77004977 | MS.gene69736:CDS | 50.0% | |
| GCAGTAGCACCATTTCCTCT+TGG | + | chr3.4:77004683-77004702 | None:intergenic | 50.0% | |
| TCTGGTGAACCTGCTCCTTT+AGG | - | chr3.4:77004731-77004750 | MS.gene69736:CDS | 50.0% | |
| GCTACTGCTACCATCCGTTG+TGG | - | chr3.4:77004695-77004714 | MS.gene69736:CDS | 55.0% | |
| ACCTGAGCAGGCGCACCTGG+AGG | + | chr3.4:77005001-77005020 | None:intergenic | 70.0% | |
| TCCACCTGAGCAGGCGCACC+TGG | + | chr3.4:77005004-77005023 | None:intergenic | 70.0% | |
| TCCAGGTGCGCCTGCTCAGG+TGG | - | chr3.4:77005000-77005019 | MS.gene69736:CDS | 70.0% | |
| TCCTCCAGGTGCGCCTGCTC+AGG | - | chr3.4:77004997-77005016 | MS.gene69736:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 77004623 | 77005078 | 77004623 | ID=MS.gene69736 |
| chr3.4 | mRNA | 77004623 | 77005078 | 77004623 | ID=MS.gene69736.t1;Parent=MS.gene69736 |
| chr3.4 | exon | 77004623 | 77005078 | 77004623 | ID=MS.gene69736.t1.exon1;Parent=MS.gene69736.t1 |
| chr3.4 | CDS | 77004623 | 77005078 | 77004623 | ID=cds.MS.gene69736.t1;Parent=MS.gene69736.t1 |
| Gene Sequence |
| Protein sequence |
