AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77301


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77301.t1 MTR_7g067380 100.000 152 0 0 13 164 347 498 1.85e-106 313
MS.gene77301.t1 MTR_4g415290 88.667 150 17 0 13 162 345 494 3.90e-93 279
MS.gene77301.t1 MTR_8g030620 69.595 148 44 1 13 159 352 499 8.73e-71 222
MS.gene77301.t1 MTR_1g040500 67.763 152 49 0 13 164 347 498 7.06e-69 218
MS.gene77301.t1 MTR_3g048330 63.087 149 55 0 13 161 129 277 2.05e-66 204
MS.gene77301.t1 MTR_5g080360 69.655 145 44 0 13 157 328 472 2.62e-66 210
MS.gene77301.t1 MTR_3g448430 62.759 145 54 0 13 157 367 511 1.72e-63 203
MS.gene77301.t1 MTR_5g091660 64.138 145 52 0 13 157 385 529 7.07e-63 202
MS.gene77301.t1 MTR_5g061520 57.718 149 62 1 15 162 338 486 3.97e-56 183
MS.gene77301.t1 MTR_1g086650 52.288 153 69 2 13 161 381 533 2.51e-49 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77301.t1 AT2G38110 86.755 151 20 0 13 163 350 500 1.25e-91 275
MS.gene77301.t1 AT1G01610 70.946 148 42 1 13 159 348 495 1.62e-71 223
MS.gene77301.t1 AT4G00400 71.622 148 41 1 13 159 347 494 2.27e-71 223
MS.gene77301.t1 AT3G11430 71.034 145 42 0 13 157 337 481 2.63e-68 215
MS.gene77301.t1 AT5G06090 68.531 143 45 0 15 157 337 479 5.13e-63 201
MS.gene77301.t1 AT1G06520 62.759 145 54 0 13 157 440 584 4.06e-60 196
MS.gene77301.t1 AT3G11325 66.935 124 41 0 34 157 246 369 4.33e-55 178
MS.gene77301.t1 AT3G11325 66.935 124 41 0 34 157 249 372 5.01e-55 178
MS.gene77301.t1 AT1G02390 51.299 154 69 1 13 160 377 530 6.11e-49 165
MS.gene77301.t1 AT4G01950 53.947 152 57 2 13 157 372 517 1.44e-46 159
MS.gene77301.t1 AT4G01950 53.947 152 57 2 13 157 400 545 2.29e-46 159

Find 39 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTAACTGTAAGCTCTTT+AGG 0.240056 7.2:-36246405 None:intergenic
GCATAATACTTTCCATCTTA+AGG 0.256062 7.2:+36245851 MS.gene77301:CDS
GGATTCATGAACACAAAATA+AGG 0.281571 7.2:-36246345 None:intergenic
CATAATACTTTCCATCTTAA+GGG 0.319065 7.2:+36245852 MS.gene77301:CDS
TCCCTACAAGTTGTTCCTTC+CGG 0.348891 7.2:-36246198 None:intergenic
CCTAAAGAGCTTACAGTTAG+TGG 0.359503 7.2:+36246405 MS.gene77301:CDS
TATATTCAAAGGGTTCTTGC+TGG 0.360292 7.2:+36246456 MS.gene77301:CDS
GATAAGTATGCTATGCTTGC+TGG 0.385223 7.2:+36246516 MS.gene77301:CDS
ATTAACCGATAGGATTGTTC+CGG 0.386364 7.2:+36246257 MS.gene77301:CDS
AGGGTTCTTGCTGGAACACT+TGG 0.417445 7.2:+36246465 MS.gene77301:CDS
ATGGAAAGTATTATGCCAAT+TGG 0.452627 7.2:-36245844 None:intergenic
TGTTTCGTGTTAATTGCAAC+CGG 0.459361 7.2:-36246276 None:intergenic
CAGTGGCACTTTCAAGAGAA+AGG 0.461557 7.2:+36246118 MS.gene77301:intron
GCGCTGAATCGTAGAAGAAA+TGG 0.469194 7.2:-36246222 None:intergenic
GTTTAGGTATACCCTTAAGA+TGG 0.476258 7.2:-36245863 None:intergenic
TCATTCCTATGGCTACCAAT+TGG 0.476350 7.2:+36245829 None:intergenic
GTCCGGAAGGAACAACTTGT+AGG 0.482795 7.2:+36246196 MS.gene77301:CDS
TGCTTGCTGGTACAGATGGT+AGG 0.502496 7.2:+36246529 MS.gene77301:CDS
AGTTGCAAATTATATTCAAA+GGG 0.504087 7.2:+36246446 MS.gene77301:CDS
AAGTTGCAAATTATATTCAA+AGG 0.504845 7.2:+36246445 MS.gene77301:CDS
ATAAGGAAATTACTTGAAGA+AGG 0.512819 7.2:+36246159 MS.gene77301:CDS
GATTCATGAACACAAAATAA+GGG 0.515157 7.2:-36246344 None:intergenic
GCTTGCTGGTACAGATGGTA+GGG 0.533473 7.2:+36246530 MS.gene77301:CDS
TCCGGAAGGAACAACTTGTA+GGG 0.535613 7.2:+36246197 MS.gene77301:CDS
AGTGGCACTTTCAAGAGAAA+GGG 0.541416 7.2:+36246119 MS.gene77301:intron
AAGAATGTGATCTCATAAGT+AGG 0.541733 7.2:-36246372 None:intergenic
GCTATGCTTGCTGGTACAGA+TGG 0.544625 7.2:+36246525 MS.gene77301:CDS
TGGTAGGGTTCCATCTAAGA+AGG 0.546476 7.2:+36246545 MS.gene77301:CDS
AGGTGACCTAGTGATTTGTC+CGG 0.552254 7.2:+36246179 MS.gene77301:CDS
GTGATCTCATAAGTAGGCAC+AGG 0.573493 7.2:-36246366 None:intergenic
GGTCCGCGGACACAAAGTAT+TGG 0.584467 7.2:+36246320 MS.gene77301:CDS
TGCAACCGGAACAATCCTAT+CGG 0.612877 7.2:-36246262 None:intergenic
TTCCTTCCGGACAAATCACT+AGG 0.618250 7.2:-36246185 None:intergenic
AAAGAGCTTACAGTTAGTGG+AGG 0.619039 7.2:+36246408 MS.gene77301:CDS
TTGAGTGTACTAATTTAACA+AGG 0.633129 7.2:+36246490 MS.gene77301:CDS
TTATGCCAATTGGTAGCCAT+AGG 0.634303 7.2:-36245834 None:intergenic
AAGAGCTTACAGTTAGTGGA+GGG 0.643335 7.2:+36246409 MS.gene77301:CDS
GGTCCAATACTTTGTGTCCG+CGG 0.653361 7.2:-36246323 None:intergenic
GACCTAGTGATTTGTCCGGA+AGG 0.669947 7.2:+36246183 MS.gene77301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTGCAAATTATATTCAA+AGG + chr7.2:36246445-36246464 MS.gene77301:CDS 20.0%
!! AGTTGCAAATTATATTCAAA+GGG + chr7.2:36246446-36246465 MS.gene77301:CDS 20.0%
!! TGGTACAATTATAAACTATT+AGG + chr7.2:36245910-36245929 MS.gene77301:intron 20.0%
! AAAAAGATGCAGCAAATATA+AGG + chr7.2:36246142-36246161 MS.gene77301:CDS 25.0%
! AGGAATCAAAGTAATTGTTA+AGG + chr7.2:36245930-36245949 MS.gene77301:intron 25.0%
! ATAAGGAAATTACTTGAAGA+AGG + chr7.2:36246159-36246178 MS.gene77301:CDS 25.0%
! CATAATACTTTCCATCTTAA+GGG + chr7.2:36245852-36245871 MS.gene77301:CDS 25.0%
! GATTCATGAACACAAAATAA+GGG - chr7.2:36246347-36246366 None:intergenic 25.0%
! GGAATCAAAGTAATTGTTAA+GGG + chr7.2:36245931-36245950 MS.gene77301:intron 25.0%
! TTGAGTGTACTAATTTAACA+AGG + chr7.2:36246490-36246509 MS.gene77301:CDS 25.0%
AAGAATGTGATCTCATAAGT+AGG - chr7.2:36246375-36246394 None:intergenic 30.0%
AATTTCACCAATTAAAGCAG+TGG + chr7.2:36246101-36246120 MS.gene77301:intron 30.0%
GCATAATACTTTCCATCTTA+AGG + chr7.2:36245851-36245870 MS.gene77301:CDS 30.0%
GGATTCATGAACACAAAATA+AGG - chr7.2:36246348-36246367 None:intergenic 30.0%
! AAGTGTGTTTTATGGTACAA+CGG + chr7.2:36246299-36246318 MS.gene77301:CDS 30.0%
! ACGAAACAAAGTGTGTTTTA+TGG + chr7.2:36246291-36246310 MS.gene77301:CDS 30.0%
!! ATGGAAAGTATTATGCCAAT+TGG - chr7.2:36245847-36245866 None:intergenic 30.0%
!!! CAAGCAATTTTTTCTGGTAA+GGG - chr7.2:36245892-36245911 None:intergenic 30.0%
!!! TTGTACCAAGCAATTTTTTC+TGG - chr7.2:36245898-36245917 None:intergenic 30.0%
ATTAACCGATAGGATTGTTC+CGG + chr7.2:36246257-36246276 MS.gene77301:CDS 35.0%
CCTTACCAGAAAAAATTGCT+TGG + chr7.2:36245890-36245909 MS.gene77301:intron 35.0%
GTTTAGGTATACCCTTAAGA+TGG - chr7.2:36245866-36245885 None:intergenic 35.0%
TATATTCAAAGGGTTCTTGC+TGG + chr7.2:36246456-36246475 MS.gene77301:CDS 35.0%
TGTTTCGTGTTAATTGCAAC+CGG - chr7.2:36246279-36246298 None:intergenic 35.0%
! ACACCTTTTTGACCTTTTCT+TGG - chr7.2:36245973-36245992 None:intergenic 35.0%
! TTTCTGGTAAGGGAATGTTT+AGG - chr7.2:36245882-36245901 None:intergenic 35.0%
! TTTTCGCTGAATTAACCGAT+AGG + chr7.2:36246247-36246266 MS.gene77301:CDS 35.0%
!! CAAGCTTTTTCCTTCTTAGA+TGG - chr7.2:36246558-36246577 None:intergenic 35.0%
!!! CCAAGCAATTTTTTCTGGTA+AGG - chr7.2:36245893-36245912 None:intergenic 35.0%
AAAGAGCTTACAGTTAGTGG+AGG + chr7.2:36246408-36246427 MS.gene77301:CDS 40.0%
AAGAGCTTACAGTTAGTGGA+GGG + chr7.2:36246409-36246428 MS.gene77301:CDS 40.0%
AGTGGCACTTTCAAGAGAAA+GGG + chr7.2:36246119-36246138 MS.gene77301:intron 40.0%
CCACTAACTGTAAGCTCTTT+AGG - chr7.2:36246408-36246427 None:intergenic 40.0%
CCTAAAGAGCTTACAGTTAG+TGG + chr7.2:36246405-36246424 MS.gene77301:CDS 40.0%
GATAAGTATGCTATGCTTGC+TGG + chr7.2:36246516-36246535 MS.gene77301:CDS 40.0%
GCACCAAGAAAAGGTCAAAA+AGG + chr7.2:36245967-36245986 MS.gene77301:intron 40.0%
!! GAAAGTGCCACTGCTTTAAT+TGG - chr7.2:36246111-36246130 None:intergenic 40.0%
!! TTTTGACCTTTTCTTGGTGC+TGG - chr7.2:36245967-36245986 None:intergenic 40.0%
AATGCAACTGCTGTAACCAC+AGG - chr7.2:36246030-36246049 None:intergenic 45.0%
ATGCAACTGCTGTAACCACA+GGG - chr7.2:36246029-36246048 None:intergenic 45.0%
CAGTGGCACTTTCAAGAGAA+AGG + chr7.2:36246118-36246137 MS.gene77301:intron 45.0%
GCGCTGAATCGTAGAAGAAA+TGG - chr7.2:36246225-36246244 None:intergenic 45.0%
GTGATCTCATAAGTAGGCAC+AGG - chr7.2:36246369-36246388 None:intergenic 45.0%
GTGGTTACAGCAGTTGCATT+AGG + chr7.2:36246030-36246049 MS.gene77301:intron 45.0%
TCCCTACAAGTTGTTCCTTC+CGG - chr7.2:36246201-36246220 None:intergenic 45.0%
TCCGGAAGGAACAACTTGTA+GGG + chr7.2:36246197-36246216 MS.gene77301:CDS 45.0%
TGCAACCGGAACAATCCTAT+CGG - chr7.2:36246265-36246284 None:intergenic 45.0%
TGGTAGGGTTCCATCTAAGA+AGG + chr7.2:36246545-36246564 MS.gene77301:CDS 45.0%
TTCCTTCCGGACAAATCACT+AGG - chr7.2:36246188-36246207 None:intergenic 45.0%
! AGGTGACCTAGTGATTTGTC+CGG + chr7.2:36246179-36246198 MS.gene77301:CDS 45.0%
! CAGGGTCTAAAATTGTGCGA+TGG - chr7.2:36246011-36246030 None:intergenic 45.0%
! TCGCACAATTTTAGACCCTG+TGG + chr7.2:36246011-36246030 MS.gene77301:intron 45.0%
! TTTTATGGTACAACGGTCCG+CGG + chr7.2:36246306-36246325 MS.gene77301:CDS 45.0%
AGGGTTCTTGCTGGAACACT+TGG + chr7.2:36246465-36246484 MS.gene77301:CDS 50.0%
GGTCCAATACTTTGTGTCCG+CGG - chr7.2:36246326-36246345 None:intergenic 50.0%
GTCCGGAAGGAACAACTTGT+AGG + chr7.2:36246196-36246215 MS.gene77301:CDS 50.0%
! GACCTAGTGATTTGTCCGGA+AGG + chr7.2:36246183-36246202 MS.gene77301:CDS 50.0%
! GCTATGCTTGCTGGTACAGA+TGG + chr7.2:36246525-36246544 MS.gene77301:CDS 50.0%
! GCTTGCTGGTACAGATGGTA+GGG + chr7.2:36246530-36246549 MS.gene77301:CDS 50.0%
! TGCTTGCTGGTACAGATGGT+AGG + chr7.2:36246529-36246548 MS.gene77301:CDS 50.0%
!!! CTTTTCTTGGTGCTGGTGGA+GGG - chr7.2:36245960-36245979 None:intergenic 50.0%
!!! TGACCTTTTCTTGGTGCTGG+TGG - chr7.2:36245964-36245983 None:intergenic 50.0%
!!! TTTTCTTGGTGCTGGTGGAG+GGG - chr7.2:36245959-36245978 None:intergenic 50.0%
CCTCCACCAGCACCAAGAAA+AGG + chr7.2:36245958-36245977 MS.gene77301:intron 55.0%
GGTCCGCGGACACAAAGTAT+TGG + chr7.2:36246320-36246339 MS.gene77301:CDS 55.0%
!!! CCTTTTCTTGGTGCTGGTGG+AGG - chr7.2:36245961-36245980 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 36245837 36246578 36245837 ID=MS.gene77301
chr7.2 mRNA 36245837 36246578 36245837 ID=MS.gene77301.t1;Parent=MS.gene77301
chr7.2 exon 36245837 36245873 36245837 ID=MS.gene77301.t1.exon1;Parent=MS.gene77301.t1
chr7.2 CDS 36245837 36245873 36245837 ID=cds.MS.gene77301.t1;Parent=MS.gene77301.t1
chr7.2 exon 36246121 36246578 36246121 ID=MS.gene77301.t1.exon2;Parent=MS.gene77301.t1
chr7.2 CDS 36246121 36246578 36246121 ID=cds.MS.gene77301.t1;Parent=MS.gene77301.t1
Gene Sequence

>MS.gene77301

ATGGCTACCAATTGGCATAATACTTTCCATCTTAAGGGTATACCTAAACATTCCCTTACCAGAAAAAATTGCTTGGTACAATTATAAACTATTAGGAATCAAAGTAATTGTTAAGGGTACCCCTCCACCAGCACCAAGAAAAGGTCAAAAAGGTGTATTATTTGTGTGTAACCATCGCACAATTTTAGACCCTGTGGTTACAGCAGTTGCATTAGGAAGAAAAATTAGTTGTGTCACATATAGTATTAGTAAATTTAGTGAAATAATTTCACCAATTAAAGCAGTGGCACTTTCAAGAGAAAGGGAAAAAGATGCAGCAAATATAAGGAAATTACTTGAAGAAGGTGACCTAGTGATTTGTCCGGAAGGAACAACTTGTAGGGAACCATTTCTTCTACGATTCAGCGCGCTTTTCGCTGAATTAACCGATAGGATTGTTCCGGTTGCAATTAACACGAAACAAAGTGTGTTTTATGGTACAACGGTCCGCGGACACAAAGTATTGGACCCTTATTTTGTGTTCATGAATCCTGTGCCTACTTATGAGATCACATTCTTGAATCAACTTCCTAAAGAGCTTACAGTTAGTGGAGGGAAATCTGCTATTGAAGTTGCAAATTATATTCAAAGGGTTCTTGCTGGAACACTTGGATTTGAGTGTACTAATTTAACAAGGAAAGATAAGTATGCTATGCTTGCTGGTACAGATGGTAGGGTTCCATCTAAGAAGGAAAAAGCTTGA

Protein sequence

>MS.gene77301.t1

MATNWHNTFHLKVALSREREKDAANIRKLLEEGDLVICPEGTTCREPFLLRFSALFAELTDRIVPVAINTKQSVFYGTTVRGHKVLDPYFVFMNPVPTYEITFLNQLPKELTVSGGKSAIEVANYIQRVLAGTLGFECTNLTRKDKYAMLAGTDGRVPSKKEKA