AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88173


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88173.t1 MTR_4g052010 97.340 188 5 0 1 188 1 188 2.00e-132 369
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88173.t1 AT2G27290 61.658 193 54 4 4 181 3 190 3.01e-62 191

Find 36 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCTTGGTTACTTCTTC+AGG 0.280999 4.4:-50493503 None:intergenic
ACAAAGCTGCATCTCCAATT+AGG 0.288679 4.4:+50494768 MS.gene88173:CDS
AAGGAGTTGCTGGCAAAATA+TGG 0.314060 4.4:+50494485 MS.gene88173:CDS
AGCTTCAAGACCATACTTCT+TGG 0.339383 4.4:-50493516 None:intergenic
ATGGTCTTGAAGCTGGTCTT+TGG 0.345812 4.4:+50493525 MS.gene88173:CDS
ACTGTGGGTGGAAACCTAAT+TGG 0.379765 4.4:-50494782 None:intergenic
AAAAGTCGGTACCTTTGCTT+TGG 0.406424 4.4:+50494733 MS.gene88173:CDS
TGATCAAGCCAAGGAGTTGC+TGG 0.407305 4.4:+50494475 MS.gene88173:CDS
CAATCCAACCAGCTACGATT+GGG 0.413841 4.4:-50494814 None:intergenic
TGAGCAACAAGAAGAGTTCA+AGG 0.424867 4.4:+50493369 MS.gene88173:CDS
GAAGAAGTAACCAAGAAGTA+TGG 0.441811 4.4:+50493506 MS.gene88173:CDS
CCAATCCAACCAGCTACGAT+TGG 0.459512 4.4:-50494815 None:intergenic
TGTTATGTATTAATCAATGC+TGG 0.464473 4.4:+50494560 MS.gene88173:CDS
CCAATCGTAGCTGGTTGGAT+TGG 0.470086 4.4:+50494815 MS.gene88173:CDS
TTCAGGTGAAGAATTCTTTG+AGG 0.474321 4.4:-50493486 None:intergenic
AAGAAGTATGGTCTTGAAGC+TGG 0.499317 4.4:+50493518 MS.gene88173:CDS
TGTTCAAACTTTGCTTCAGA+AGG 0.506472 4.4:+50494589 MS.gene88173:CDS
GCTCTTACCCCAATCGTAGC+TGG 0.519207 4.4:+50494806 MS.gene88173:CDS
GGGGAACAACAGAAATCAAA+AGG 0.520589 4.4:+50494452 MS.gene88173:CDS
GGAATCACTACTGATGCAAC+CGG 0.523755 4.4:+50494707 MS.gene88173:CDS
TTACCCCAATCGTAGCTGGT+TGG 0.540207 4.4:+50494810 MS.gene88173:CDS
TTTCAGTTCAAAGGAAGAAG+GGG 0.549822 4.4:+50494433 MS.gene88173:CDS
AGAGATCAATGACAGAGTAA+TGG 0.554810 4.4:-50494528 None:intergenic
TCTTGAAGCTGGTCTTTGGA+AGG 0.564484 4.4:+50493529 MS.gene88173:CDS
AAAGAAGCACAAGAAGGTGA+TGG 0.588002 4.4:-50493344 None:intergenic
CAATGACAGAGTAATGGAAG+TGG 0.589427 4.4:-50494522 None:intergenic
CTCAGAAAAGAAGCACAAGA+AGG 0.593610 4.4:-50493350 None:intergenic
ATCAAAAGGTGATCAAGCCA+AGG 0.614436 4.4:+50494466 MS.gene88173:CDS
GGGGTAAGAGCTACTGTGGG+TGG 0.621082 4.4:-50494794 None:intergenic
ATTAGATTTACGTGACTGAG+TGG 0.639111 4.4:-50493408 None:intergenic
TGCAGCATATGCCAAAGCAA+AGG 0.641846 4.4:-50494744 None:intergenic
GATTGGGGTAAGAGCTACTG+TGG 0.646696 4.4:-50494798 None:intergenic
AATCCAACCAGCTACGATTG+GGG 0.648062 4.4:-50494813 None:intergenic
GAGTTGCTGGCAAAATATGG+AGG 0.682334 4.4:+50494488 MS.gene88173:CDS
ATTGGGGTAAGAGCTACTGT+GGG 0.725389 4.4:-50494797 None:intergenic
AGCTGTTAAAGAGAAAACTG+AGG 0.740712 4.4:+50493451 MS.gene88173:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATGAAATTTCTCAATT+TGG - chr4.4:50493629-50493648 None:intergenic 15.0%
!! TCTAAATTTCTACTTTAAAT+TGG - chr4.4:50494066-50494085 None:intergenic 15.0%
!! TGTTTGTAAAATGAAAATTA+TGG + chr4.4:50494398-50494417 MS.gene88173:intron 15.0%
!!! AAATATAAGTTGAGTTTTTA+AGG + chr4.4:50494192-50494211 MS.gene88173:intron 15.0%
!!! ATTGAGAAATTTCATATTTT+TGG + chr4.4:50493630-50493649 MS.gene88173:intron 15.0%
!!! TTGAGAAATTTCATATTTTT+GGG + chr4.4:50493631-50493650 MS.gene88173:intron 15.0%
!! AAACCTAAAATAAAACTCAA+TGG - chr4.4:50493883-50493902 None:intergenic 20.0%
!! AAATAAACAAGAGAAAGAAA+TGG - chr4.4:50493558-50493577 None:intergenic 20.0%
!! AATAAACAAGAGAAAGAAAT+GGG - chr4.4:50493557-50493576 None:intergenic 20.0%
!! ATTATACTTAAATCCACATT+GGG - chr4.4:50494633-50494652 None:intergenic 20.0%
!! TTAGTATAGAGTATATATGA+AGG + chr4.4:50493673-50493692 MS.gene88173:intron 20.0%
!! TTGGTTTAATTATGATTATG+TGG + chr4.4:50493577-50493596 MS.gene88173:intron 20.0%
!!! ATGTCACTTTATCAATTATT+TGG + chr4.4:50493850-50493869 MS.gene88173:intron 20.0%
!!! TGTCACTTTATCAATTATTT+GGG + chr4.4:50493851-50493870 MS.gene88173:intron 20.0%
!!! TTACCATTGAGTTTTATTTT+AGG + chr4.4:50493877-50493896 MS.gene88173:intron 20.0%
!!! TTTCTTTCTCTTGTTTATTT+TGG + chr4.4:50493558-50493577 MS.gene88173:intron 20.0%
!!! TTTTATTACTGAAATTTGCA+GGG + chr4.4:50493712-50493731 MS.gene88173:intron 20.0%
! ACTAATGATTGAAAATCGTT+TGG - chr4.4:50494324-50494343 None:intergenic 25.0%
! CATTATACTTAAATCCACAT+TGG - chr4.4:50494634-50494653 None:intergenic 25.0%
! TATGAAGGATATTCAAAGAT+AGG + chr4.4:50493688-50493707 MS.gene88173:intron 25.0%
! TGTTATGTATTAATCAATGC+TGG + chr4.4:50494560-50494579 MS.gene88173:CDS 25.0%
!! ATTTGTTTCCTTGATTTTTG+CGG + chr4.4:50494222-50494241 MS.gene88173:intron 25.0%
!! CTTTTATTACTGAAATTTGC+AGG + chr4.4:50493711-50493730 MS.gene88173:intron 25.0%
CAGTATGCTAAAACAAGAAA+TGG - chr4.4:50493911-50493930 None:intergenic 30.0%
TATCTCAGCTGTAAAATTGT+TGG - chr4.4:50494100-50494119 None:intergenic 30.0%
TTTGAACGACATTCAAACAT+TGG - chr4.4:50494024-50494043 None:intergenic 30.0%
! ATTTTCAGTTCAAAGGAAGA+AGG + chr4.4:50494431-50494450 MS.gene88173:CDS 30.0%
! TTTGCAGATTTTCAGTTCAA+AGG + chr4.4:50494424-50494443 MS.gene88173:intron 30.0%
! TTTTCAGTTCAAAGGAAGAA+GGG + chr4.4:50494432-50494451 MS.gene88173:CDS 30.0%
!! GACATGGGTTTTATACTTAT+GGG + chr4.4:50494275-50494294 MS.gene88173:intron 30.0%
!! TGACATGGGTTTTATACTTA+TGG + chr4.4:50494274-50494293 MS.gene88173:intron 30.0%
AGAGATCAATGACAGAGTAA+TGG - chr4.4:50494531-50494550 None:intergenic 35.0%
AGCTGTTAAAGAGAAAACTG+AGG + chr4.4:50493451-50493470 MS.gene88173:CDS 35.0%
ATTAGATTTACGTGACTGAG+TGG - chr4.4:50493411-50493430 None:intergenic 35.0%
CAGTACAGAACATTTCTTGA+AGG - chr4.4:50493986-50494005 None:intergenic 35.0%
CATTATGTCTGTATATCTGC+AGG + chr4.4:50494682-50494701 MS.gene88173:intron 35.0%
GAAGAAGTAACCAAGAAGTA+TGG + chr4.4:50493506-50493525 MS.gene88173:CDS 35.0%
GAGAGGATGAATAATGACAT+GGG + chr4.4:50494260-50494279 MS.gene88173:intron 35.0%
GCTGTAAAATTGTTGGAACT+TGG - chr4.4:50494093-50494112 None:intergenic 35.0%
TACTTCTTGGTTACTTCTTC+AGG - chr4.4:50493506-50493525 None:intergenic 35.0%
TGTTCAAACTTTGCTTCAGA+AGG + chr4.4:50494589-50494608 MS.gene88173:CDS 35.0%
TTACTGAAATTTGCAGGGAA+AGG + chr4.4:50493717-50493736 MS.gene88173:intron 35.0%
TTCAGGTGAAGAATTCTTTG+AGG - chr4.4:50493489-50493508 None:intergenic 35.0%
TTTACAAACAACGAAAAGCG+CGG - chr4.4:50494388-50494407 None:intergenic 35.0%
TTTCAGTTCAAAGGAAGAAG+GGG + chr4.4:50494433-50494452 MS.gene88173:CDS 35.0%
! CTTTTGTTCCCCTAAAATCA+GGG - chr4.4:50493941-50493960 None:intergenic 35.0%
! TCTTTTGTTCCCCTAAAATC+AGG - chr4.4:50493942-50493961 None:intergenic 35.0%
! TGGGTTGCTTCTTATTTATG+TGG + chr4.4:50493650-50493669 MS.gene88173:intron 35.0%
!! TTTGCGGACTATCGTTTTAA+TGG + chr4.4:50494238-50494257 MS.gene88173:intron 35.0%
AAAAGTCGGTACCTTTGCTT+TGG + chr4.4:50494733-50494752 MS.gene88173:CDS 40.0%
AAATTGGCGACACAAGTCAT+CGG - chr4.4:50494050-50494069 None:intergenic 40.0%
AAGGAGTTGCTGGCAAAATA+TGG + chr4.4:50494485-50494504 MS.gene88173:CDS 40.0%
ACAAAGCTGCATCTCCAATT+AGG + chr4.4:50494768-50494787 MS.gene88173:CDS 40.0%
ACGATAGTCCGCAAAAATCA+AGG - chr4.4:50494233-50494252 None:intergenic 40.0%
AGCTTCAAGACCATACTTCT+TGG - chr4.4:50493519-50493538 None:intergenic 40.0%
ATCAAAAGGTGATCAAGCCA+AGG + chr4.4:50494466-50494485 MS.gene88173:CDS 40.0%
ATGTCTGTATATCTGCAGGT+CGG + chr4.4:50494686-50494705 MS.gene88173:intron 40.0%
GGAGAGGATGAATAATGACA+TGG + chr4.4:50494259-50494278 MS.gene88173:intron 40.0%
GGGAACAAAAGATGTGTTGT+GGG + chr4.4:50493950-50493969 MS.gene88173:intron 40.0%
GGGGAACAACAGAAATCAAA+AGG + chr4.4:50494452-50494471 MS.gene88173:CDS 40.0%
TGAGCAACAAGAAGAGTTCA+AGG + chr4.4:50493369-50493388 MS.gene88173:CDS 40.0%
TGCTCATACATAACCCAATG+TGG + chr4.4:50494617-50494636 MS.gene88173:intron 40.0%
TGCTTGTCCATGTAGAACTT+AGG + chr4.4:50493790-50493809 MS.gene88173:intron 40.0%
! AAAGAAGCACAAGAAGGTGA+TGG - chr4.4:50493347-50493366 None:intergenic 40.0%
! AAGAAGTATGGTCTTGAAGC+TGG + chr4.4:50493518-50493537 MS.gene88173:CDS 40.0%
! ATATTTTGCCAGCAACTCCT+TGG - chr4.4:50494486-50494505 None:intergenic 40.0%
! CAATGACAGAGTAATGGAAG+TGG - chr4.4:50494525-50494544 None:intergenic 40.0%
! CTCAGAAAAGAAGCACAAGA+AGG - chr4.4:50493353-50493372 None:intergenic 40.0%
! TGCATCGTTCCCTGATTTTA+GGG + chr4.4:50493929-50493948 MS.gene88173:intron 40.0%
!! GGACTATCGTTTTAATGGAG+AGG + chr4.4:50494243-50494262 MS.gene88173:intron 40.0%
AATCCAACCAGCTACGATTG+GGG - chr4.4:50494816-50494835 None:intergenic 45.0%
ACTGTGGGTGGAAACCTAAT+TGG - chr4.4:50494785-50494804 None:intergenic 45.0%
ATTGGGGTAAGAGCTACTGT+GGG - chr4.4:50494800-50494819 None:intergenic 45.0%
CAATCCAACCAGCTACGATT+GGG - chr4.4:50494817-50494836 None:intergenic 45.0%
GAGACAGCCTAAGTTCTACA+TGG - chr4.4:50493800-50493819 None:intergenic 45.0%
GAGTTGCTGGCAAAATATGG+AGG + chr4.4:50494488-50494507 MS.gene88173:CDS 45.0%
GGAATCACTACTGATGCAAC+CGG + chr4.4:50494707-50494726 MS.gene88173:CDS 45.0%
GGGGAACAAAAGATGTGTTG+TGG + chr4.4:50493949-50493968 MS.gene88173:intron 45.0%
TGCAGCATATGCCAAAGCAA+AGG - chr4.4:50494747-50494766 None:intergenic 45.0%
! AAAAGCGCGGCTTTGACTTT+AGG - chr4.4:50494375-50494394 None:intergenic 45.0%
! AAAGCGCGGCTTTGACTTTA+GGG - chr4.4:50494374-50494393 None:intergenic 45.0%
! CTGCATCGTTCCCTGATTTT+AGG + chr4.4:50493928-50493947 MS.gene88173:intron 45.0%
! GCATCGTTCCCTGATTTTAG+GGG + chr4.4:50493930-50493949 MS.gene88173:intron 45.0%
!! ATGGTCTTGAAGCTGGTCTT+TGG + chr4.4:50493525-50493544 MS.gene88173:CDS 45.0%
!! TCTTGAAGCTGGTCTTTGGA+AGG + chr4.4:50493529-50493548 MS.gene88173:CDS 45.0%
!! TTATGGGAGTGTCATCGTGT+CGG + chr4.4:50494291-50494310 MS.gene88173:intron 45.0%
!!! AAAGGTACCGACTTTTTCGC+CGG - chr4.4:50494729-50494748 None:intergenic 45.0%
CCAATCCAACCAGCTACGAT+TGG - chr4.4:50494818-50494837 None:intergenic 50.0%
CCAATCGTAGCTGGTTGGAT+TGG + chr4.4:50494815-50494834 MS.gene88173:CDS 50.0%
GATGCAACCGGCGAAAAAGT+CGG + chr4.4:50494719-50494738 MS.gene88173:CDS 50.0%
GATTGGGGTAAGAGCTACTG+TGG - chr4.4:50494801-50494820 None:intergenic 50.0%
TGATCAAGCCAAGGAGTTGC+TGG + chr4.4:50494475-50494494 MS.gene88173:CDS 50.0%
TTACCCCAATCGTAGCTGGT+TGG + chr4.4:50494810-50494829 MS.gene88173:CDS 50.0%
GCTCTTACCCCAATCGTAGC+TGG + chr4.4:50494806-50494825 MS.gene88173:CDS 55.0%
GGGGTAAGAGCTACTGTGGG+TGG - chr4.4:50494797-50494816 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 50493323 50494862 50493323 ID=MS.gene88173
chr4.4 mRNA 50493323 50494862 50493323 ID=MS.gene88173.t1;Parent=MS.gene88173
chr4.4 exon 50493323 50493550 50493323 ID=MS.gene88173.t1.exon1;Parent=MS.gene88173.t1
chr4.4 CDS 50493323 50493550 50493323 ID=cds.MS.gene88173.t1;Parent=MS.gene88173.t1
chr4.4 exon 50494431 50494610 50494431 ID=MS.gene88173.t1.exon2;Parent=MS.gene88173.t1
chr4.4 CDS 50494431 50494610 50494431 ID=cds.MS.gene88173.t1;Parent=MS.gene88173.t1
chr4.4 exon 50494704 50494862 50494704 ID=MS.gene88173.t1.exon3;Parent=MS.gene88173.t1
chr4.4 CDS 50494704 50494862 50494704 ID=cds.MS.gene88173.t1;Parent=MS.gene88173.t1
Gene Sequence

>MS.gene88173

ATGACAACGTTTTTGTTGCTACCATCACCTTCTTGTGCTTCTTTTCTGAGCAACAAGAAGAGTTCAAGGTTTTGCAGTCTTGCTTCCACTCAGTCACGTAAATCTAATGTTAAAGTGTTGAGAGTTAGAGCTGTTAAAGAGAAAACTGAGGAAATCAAAAGCTCCTCAAAGAATTCTTCACCTGAAGAAGTAACCAAGAAGTATGGTCTTGAAGCTGGTCTTTGGAAGGTGCCCATTTCTTTCTCTTGTTTATTTTGGTTTAATTATGATTATGTGGTTAGTATAAAGTTTTAATTTCATTAACCAAATTGAGAAATTTCATATTTTTGGGTTGCTTCTTATTTATGTGGTTAGTATAGAGTATATATGAAGGATATTCAAAGATAGGCTTTTATTACTGAAATTTGCAGGGAAAGGAAATAAAGAAAGAATTTAGTTTGAACAAGTTAAGACAGAATGAGTAGTTTTGCTTGTCCATGTAGAACTTAGGCTGTCTCAGATTAAATGTTGCGTTTAAAACAAGAGATATGTCACTTTATCAATTATTTGGGTGTTTACCATTGAGTTTTATTTTAGGTTTTCTTTCCATTTCTTGTTTTAGCATACTGCATCGTTCCCTGATTTTAGGGGAACAAAAGATGTGTTGTGGGTGAAATTTTACCTTCAAGAAATGTTCTGTACTGAATCAAGATTGAGTACCAATGTTTGAATGTCGTTCAAAATTCCGATGACTTGTGTCGCCAATTTAAAGTAGAAATTTAGAATTTCCAAGTTCCAACAATTTTACAGCTGAGATAGTAATTTAATCAGTGTTTTTTTTTTTTTTGACAAATTAATTTAATCAGTGTTGATTTGTTAAAAATTATCAGAAATATAAGTTGAGTTTTTAAGGTAGTGAAATTTGTTTCCTTGATTTTTGCGGACTATCGTTTTAATGGAGAGGATGAATAATGACATGGGTTTTATACTTATGGGAGTGTCATCGTGTCGGTAGAGCTCCAAACGATTTTCAATCATTAGTTTACTGTAGACTATACGAGACACTTCACCCTAAAGTCAAAGCCGCGCTTTTCGTTGTTTGTAAAATGAAAATTATGGTCTTTTGCAGATTTTCAGTTCAAAGGAAGAAGGGGAACAACAGAAATCAAAAGGTGATCAAGCCAAGGAGTTGCTGGCAAAATATGGAGGAGCATACTTAGCCACTTCCATTACTCTGTCATTGATCTCTTTCGCTCTTTGTTATGTATTAATCAATGCTGGAGTAGATGTTCAAACTTTGCTTCAGAAGGTGCAGTGCTCATACATAACCCAATGTGGATTTAAGTATAATGAAAACAAAGTAACTTCTATGAATTAACACATTATGTCTGTATATCTGCAGGTCGGAATCACTACTGATGCAACCGGCGAAAAAGTCGGTACCTTTGCTTTGGCATATGCTGCACACAAAGCTGCATCTCCAATTAGGTTTCCACCCACAGTAGCTCTTACCCCAATCGTAGCTGGTTGGATTGGAAAGAAAGCTGATAAAGATAAGTAG

Protein sequence

>MS.gene88173.t1

MTTFLLLPSPSCASFLSNKKSSRFCSLASTQSRKSNVKVLRVRAVKEKTEEIKSSSKNSSPEEVTKKYGLEAGLWKIFSSKEEGEQQKSKGDQAKELLAKYGGAYLATSITLSLISFALCYVLINAGVDVQTLLQKVGITTDATGEKVGTFALAYAAHKAASPIRFPPTVALTPIVAGWIGKKADKDK