Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99674.t1 | RDX75728.1 | 86.4 | 509 | 69 | 0 | 9 | 517 | 116 | 624 | 1.90E-269 | 937.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99674.t1 | Q8H1Z0 | 70.9 | 508 | 145 | 2 | 6 | 510 | 122 | 629 | 1.2e-216 | 753.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99674.t1 | A0A371FBR1 | 86.4 | 509 | 69 | 0 | 9 | 517 | 116 | 624 | 1.4e-269 | 937.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049267 | MS.gene99674 | -0.802154 | 6.42E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene99674 | 0.836807 | 7.54E-57 | -1.69E-46 |
| MS.gene049369 | MS.gene99674 | 0.841274 | 5.25E-58 | -1.69E-46 |
| MS.gene049396 | MS.gene99674 | 0.83883 | 2.28E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene99674 | 0.811321 | 7.42E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene99674 | 0.801159 | 1.03E-48 | -1.69E-46 |
| MS.gene050122 | MS.gene99674 | 0.8302 | 3.36E-55 | -1.69E-46 |
| MS.gene050380 | MS.gene99674 | -0.814835 | 1.26E-51 | -1.69E-46 |
| MS.gene050499 | MS.gene99674 | 0.835652 | 1.48E-56 | -1.69E-46 |
| MS.gene050520 | MS.gene99674 | 0.826905 | 2.10E-54 | -1.69E-46 |
| MS.gene050593 | MS.gene99674 | 0.821943 | 3.09E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene99674 | 0.835086 | 2.06E-56 | -1.69E-46 |
| MS.gene050686 | MS.gene99674 | 0.825539 | 4.44E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene99674 | 0.813757 | 2.18E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene99674 | 0.845845 | 3.15E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene99674 | 0.857281 | 1.82E-62 | -1.69E-46 |
| MS.gene051255 | MS.gene99674 | 0.845424 | 4.10E-59 | -1.69E-46 |
| MS.gene051360 | MS.gene99674 | 0.808505 | 3.00E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene99674 | 0.814832 | 1.26E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene99674 | 0.805928 | 1.05E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene99674 | 0.808908 | 2.46E-50 | -1.69E-46 |
| MS.gene051874 | MS.gene99674 | 0.824641 | 7.23E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene99674 | 0.805282 | 1.44E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene99674 | 0.811137 | 8.14E-51 | -1.69E-46 |
| MS.gene052346 | MS.gene99674 | 0.828441 | 8.98E-55 | -1.69E-46 |
| MS.gene052414 | MS.gene99674 | 0.823889 | 1.09E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene99674 | 0.820637 | 6.17E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene99674 | 0.82889 | 6.99E-55 | -1.69E-46 |
| MS.gene052999 | MS.gene99674 | 0.869673 | 2.60E-66 | -1.69E-46 |
| MS.gene053018 | MS.gene99674 | 0.808885 | 2.49E-50 | -1.69E-46 |
| MS.gene053169 | MS.gene99674 | 0.822904 | 1.85E-53 | -1.69E-46 |
| MS.gene05335 | MS.gene99674 | 0.821422 | 4.07E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene99674 | 0.858194 | 9.74E-63 | -1.69E-46 |
| MS.gene053505 | MS.gene99674 | 0.817366 | 3.42E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene99674 | 0.810587 | 1.07E-50 | -1.69E-46 |
| MS.gene053758 | MS.gene99674 | 0.841455 | 4.70E-58 | -1.69E-46 |
| MS.gene053813 | MS.gene99674 | 0.878365 | 2.98E-69 | -1.69E-46 |
| MS.gene053912 | MS.gene99674 | 0.82762 | 1.41E-54 | -1.69E-46 |
| MS.gene054010 | MS.gene99674 | 0.845043 | 5.19E-59 | -1.69E-46 |
| MS.gene054011 | MS.gene99674 | 0.836492 | 9.07E-57 | -1.69E-46 |
| MS.gene054012 | MS.gene99674 | 0.843386 | 1.45E-58 | -1.69E-46 |
| MS.gene054013 | MS.gene99674 | 0.845347 | 4.30E-59 | -1.69E-46 |
| MS.gene054486 | MS.gene99674 | 0.817155 | 3.82E-52 | -1.69E-46 |
| MS.gene054556 | MS.gene99674 | 0.818428 | 1.97E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene99674 | 0.825929 | 3.58E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene99674 | 0.825559 | 4.39E-54 | -1.69E-46 |
| MS.gene055604 | MS.gene99674 | 0.814439 | 1.54E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene99674 | 0.815266 | 1.01E-51 | -1.69E-46 |
| MS.gene055741 | MS.gene99674 | 0.828355 | 9.42E-55 | -1.69E-46 |
| MS.gene055744 | MS.gene99674 | 0.831886 | 1.29E-55 | -1.69E-46 |
| MS.gene055909 | MS.gene99674 | 0.866159 | 3.50E-65 | -1.69E-46 |
| MS.gene055911 | MS.gene99674 | 0.87276 | 2.48E-67 | -1.69E-46 |
| MS.gene05592 | MS.gene99674 | 0.801413 | 9.11E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene99674 | 0.871947 | 4.63E-67 | -1.69E-46 |
| MS.gene056346 | MS.gene99674 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene056410 | MS.gene99674 | 0.813234 | 2.84E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene99674 | 0.865799 | 4.54E-65 | -1.69E-46 |
| MS.gene056448 | MS.gene99674 | 0.820146 | 8.00E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene99674 | 0.842912 | 1.93E-58 | -1.69E-46 |
| MS.gene056833 | MS.gene99674 | 0.819085 | 1.40E-52 | -1.69E-46 |
| MS.gene056902 | MS.gene99674 | 0.828198 | 1.03E-54 | -1.69E-46 |
| MS.gene056942 | MS.gene99674 | 0.82752 | 1.49E-54 | -1.69E-46 |
| MS.gene057076 | MS.gene99674 | 0.820716 | 5.92E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene99674 | 0.800614 | 1.33E-48 | -1.69E-46 |
| MS.gene057289 | MS.gene99674 | 0.812705 | 3.71E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene99674 | 0.80918 | 2.15E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene99674 | 0.846402 | 2.22E-59 | -1.69E-46 |
| MS.gene057823 | MS.gene99674 | 0.850885 | 1.27E-60 | -1.69E-46 |
| MS.gene058007 | MS.gene99674 | 0.808809 | 2.58E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene99674 | 0.804827 | 1.79E-49 | -1.69E-46 |
| MS.gene058459 | MS.gene99674 | 0.805132 | 1.55E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene99674 | 0.805805 | 1.12E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene99674 | 0.834048 | 3.75E-56 | -1.69E-46 |
| MS.gene058948 | MS.gene99674 | 0.805136 | 1.54E-49 | -1.69E-46 |
| MS.gene058995 | MS.gene99674 | 0.861637 | 8.92E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene99674 | 0.866801 | 2.19E-65 | -1.69E-46 |
| MS.gene059366 | MS.gene99674 | 0.831939 | 1.26E-55 | -1.69E-46 |
| MS.gene059368 | MS.gene99674 | 0.842636 | 2.29E-58 | -1.69E-46 |
| MS.gene059402 | MS.gene99674 | -0.800766 | 1.24E-48 | -1.69E-46 |
| MS.gene059420 | MS.gene99674 | 0.840026 | 1.11E-57 | -1.69E-46 |
| MS.gene059517 | MS.gene99674 | 0.800882 | 1.17E-48 | -1.69E-46 |
| MS.gene059536 | MS.gene99674 | 0.801609 | 8.31E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene99674 | 0.851426 | 8.95E-61 | -1.69E-46 |
| MS.gene059680 | MS.gene99674 | 0.810761 | 9.81E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene99674 | 0.820235 | 7.64E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene99674 | 0.857207 | 1.91E-62 | -1.69E-46 |
| MS.gene059807 | MS.gene99674 | 0.841682 | 4.10E-58 | -1.69E-46 |
| MS.gene059974 | MS.gene99674 | 0.828051 | 1.11E-54 | -1.69E-46 |
| MS.gene060372 | MS.gene99674 | 0.854287 | 1.36E-61 | -1.69E-46 |
| MS.gene060401 | MS.gene99674 | 0.804864 | 1.76E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene99674 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene060482 | MS.gene99674 | 0.816538 | 5.25E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene99674 | 0.828948 | 6.77E-55 | -1.69E-46 |
| MS.gene060837 | MS.gene99674 | 0.879769 | 9.51E-70 | -1.69E-46 |
| MS.gene061154 | MS.gene99674 | 0.832692 | 8.18E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99674.t1 | MTR_4g129630 | 79.377 | 514 | 104 | 1 | 6 | 517 | 120 | 633 | 0.0 | 880 |
| MS.gene99674.t1 | MTR_8g009590 | 37.624 | 505 | 306 | 5 | 6 | 509 | 157 | 653 | 1.68e-109 | 340 |
| MS.gene99674.t1 | MTR_8g009560 | 34.394 | 503 | 322 | 4 | 6 | 508 | 121 | 615 | 3.00e-98 | 310 |
| MS.gene99674.t1 | MTR_7g090100 | 34.190 | 506 | 318 | 5 | 7 | 510 | 119 | 611 | 1.56e-93 | 297 |
| MS.gene99674.t1 | MTR_7g090120 | 33.661 | 508 | 320 | 5 | 7 | 510 | 119 | 613 | 5.64e-92 | 293 |
| MS.gene99674.t1 | MTR_7g090140 | 34.064 | 502 | 321 | 4 | 7 | 508 | 24 | 515 | 3.39e-91 | 288 |
| MS.gene99674.t1 | MTR_4g054290 | 34.592 | 503 | 318 | 5 | 7 | 508 | 119 | 611 | 1.52e-90 | 289 |
| MS.gene99674.t1 | MTR_7g090140 | 34.064 | 502 | 321 | 4 | 7 | 508 | 119 | 610 | 8.18e-90 | 288 |
| MS.gene99674.t1 | MTR_4g054150 | 31.076 | 502 | 316 | 5 | 7 | 508 | 119 | 590 | 1.25e-84 | 273 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99674.t1 | AT5G57800 | 70.866 | 508 | 145 | 2 | 6 | 510 | 122 | 629 | 0.0 | 762 |
| MS.gene99674.t1 | AT1G02205 | 37.103 | 504 | 310 | 5 | 7 | 508 | 123 | 621 | 2.46e-110 | 341 |
| MS.gene99674.t1 | AT1G02205 | 37.103 | 504 | 310 | 5 | 7 | 508 | 122 | 620 | 4.41e-110 | 340 |
| MS.gene99674.t1 | AT1G02205 | 36.614 | 508 | 312 | 5 | 7 | 508 | 122 | 625 | 1.16e-108 | 337 |
| MS.gene99674.t1 | AT1G02205 | 36.547 | 446 | 276 | 5 | 65 | 508 | 16 | 456 | 1.10e-100 | 311 |
| MS.gene99674.t1 | AT2G37700 | 35.050 | 505 | 318 | 5 | 7 | 508 | 118 | 615 | 7.76e-97 | 306 |
| MS.gene99674.t1 | AT1G02190 | 35.798 | 514 | 305 | 8 | 7 | 508 | 122 | 622 | 1.52e-96 | 305 |
| MS.gene99674.t1 | AT2G37700 | 34.851 | 505 | 310 | 5 | 7 | 508 | 118 | 606 | 7.45e-96 | 303 |
| MS.gene99674.t1 | AT1G02190 | 35.603 | 514 | 302 | 9 | 7 | 508 | 122 | 618 | 1.54e-94 | 300 |
| MS.gene99674.t1 | AT1G02205 | 36.148 | 379 | 235 | 5 | 132 | 508 | 8 | 381 | 8.86e-81 | 257 |
| MS.gene99674.t1 | AT2G37700 | 32.475 | 505 | 294 | 6 | 7 | 508 | 118 | 578 | 3.78e-79 | 259 |
| MS.gene99674.t1 | AT2G37700 | 35.955 | 356 | 226 | 2 | 7 | 361 | 118 | 472 | 1.39e-68 | 228 |
Find 124 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCCCTAGGTGTGGCTTTC+AGG | 0.088000 | 2.1:+39633085 | MS.gene99674:CDS |
| TCTCAGAACCACTTTATTAT+TGG | 0.179518 | 2.1:+39630026 | MS.gene99674:CDS |
| CTGTTTCTGTCGTTAGTAAT+TGG | 0.197549 | 2.1:+39630431 | MS.gene99674:CDS |
| TCACTCCAAGAGAGCAAAAT+TGG | 0.214120 | 2.1:+39635638 | MS.gene99674:CDS |
| TTTAGAAGAGATTGCACTTA+TGG | 0.262243 | 2.1:+39635712 | MS.gene99674:CDS |
| AAAACTTCTTTGGCATCTTT+AGG | 0.262452 | 2.1:-39634909 | None:intergenic |
| CTCAGAACCACTTTATTATT+GGG | 0.270378 | 2.1:+39630027 | MS.gene99674:CDS |
| CAGCTTGGCTGCTTTGAATA+AGG | 0.272905 | 2.1:+39633371 | MS.gene99674:CDS |
| ATGCAGAATGAATCTCTAAA+TGG | 0.279050 | 2.1:+39634792 | MS.gene99674:intron |
| CATGAACAACTCGAACTCTT+AGG | 0.279627 | 2.1:-39634848 | None:intergenic |
| TATATGATATATCTCATAAA+TGG | 0.286536 | 2.1:-39630593 | None:intergenic |
| AGCGGGAACCGGTACGGTTT+TGG | 0.292315 | 2.1:+39630403 | MS.gene99674:intron |
| AGGATGTGATATCCACTATA+TGG | 0.314664 | 2.1:-39632887 | None:intergenic |
| CCCGGTAAGGAAAACTTCTT+TGG | 0.317209 | 2.1:-39634919 | None:intergenic |
| ATTCTCACCGCTGATAAAAT+TGG | 0.319998 | 2.1:+39633336 | MS.gene99674:CDS |
| AAGATAACTTTGGTATTCTT+CGG | 0.331311 | 2.1:-39635554 | None:intergenic |
| GGAGCTTCATTGATGGGATA+TGG | 0.335334 | 2.1:+39630467 | MS.gene99674:CDS |
| CTTTGTTAGCAATGTGGCTT+TGG | 0.345554 | 2.1:+39633003 | MS.gene99674:CDS |
| CTAAGAGTTCGAGTTGTTCA+TGG | 0.362503 | 2.1:+39634849 | MS.gene99674:CDS |
| GGTCTAAGACCTTTGTCTTC+GGG | 0.363383 | 2.1:+39636563 | MS.gene99674:CDS |
| AGATAACTTTGGTATTCTTC+GGG | 0.364479 | 2.1:-39635553 | None:intergenic |
| TTTAACTGCAGCTGTGATCT+TGG | 0.367863 | 2.1:+39634878 | MS.gene99674:CDS |
| CATGGCCATGGGAGAACTTT+GGG | 0.369137 | 2.1:+39629240 | None:intergenic |
| GGATCAACAAGTATGATATA+TGG | 0.369981 | 2.1:+39630488 | MS.gene99674:CDS |
| TGGGTGCATAAGAAGTTTCA+TGG | 0.372705 | 2.1:+39630046 | MS.gene99674:CDS |
| CCCGTTACTGACTGTGGTAC+TGG | 0.377406 | 2.1:-39630112 | None:intergenic |
| CCAAAAGAAAGTCAAAATTC+TGG | 0.380050 | 2.1:+39634983 | MS.gene99674:CDS |
| TGGTCTAAGACCTTTGTCTT+CGG | 0.393012 | 2.1:+39636562 | MS.gene99674:CDS |
| AAATTGGAGGAACAACAAAT+TGG | 0.393279 | 2.1:-39635684 | None:intergenic |
| ACAACTCGAACTCTTAGGTT+TGG | 0.396990 | 2.1:-39634843 | None:intergenic |
| TTTGTGTAATTCCCATGATT+TGG | 0.405516 | 2.1:-39630830 | None:intergenic |
| CAACTCGAACTCTTAGGTTT+GGG | 0.405560 | 2.1:-39634842 | None:intergenic |
| CGAATGCAGGAAATTGTAGT+AGG | 0.411595 | 2.1:+39632841 | MS.gene99674:intron |
| CCAAGGCCTTCCACATCATC+TGG | 0.420649 | 2.1:-39635757 | None:intergenic |
| TGCAGAATGAATCTCTAAAT+GGG | 0.425144 | 2.1:+39634793 | MS.gene99674:intron |
| TTACACAAATCATTCAGTTC+AGG | 0.431698 | 2.1:+39630844 | MS.gene99674:CDS |
| TCATGGCCATGGGAGAACTT+TGG | 0.442180 | 2.1:+39629239 | None:intergenic |
| TTGGAGCTATTGATGTTGAT+AGG | 0.444796 | 2.1:+39636507 | MS.gene99674:CDS |
| ATGGTGAAGTGAATGGTATT+TGG | 0.447963 | 2.1:-39630083 | None:intergenic |
| TGGGCCAGGAAAACAAATTC+CGG | 0.457449 | 2.1:-39632867 | None:intergenic |
| CAATTACTAACGACAGAAAC+AGG | 0.460695 | 2.1:-39630430 | None:intergenic |
| CCAGATGATGTGGAAGGCCT+TGG | 0.468693 | 2.1:+39635757 | MS.gene99674:CDS |
| CATTGCAAGACATGGATTGT+TGG | 0.469406 | 2.1:+39635607 | MS.gene99674:CDS |
| CACTCCAAGAGAGCAAAATT+GGG | 0.470494 | 2.1:+39635639 | MS.gene99674:CDS |
| TGACTTGAACAAGATAACTT+TGG | 0.472839 | 2.1:-39635564 | None:intergenic |
| TCTTGCAATGCTGTGCTGCT+TGG | 0.479530 | 2.1:-39635594 | None:intergenic |
| CCAGTACCACAGTCAGTAAC+GGG | 0.482696 | 2.1:+39630112 | MS.gene99674:CDS |
| AACAAAGCTACCACGGCCAT+TGG | 0.484099 | 2.1:-39632987 | None:intergenic |
| GGAGAACTTTGGGATATTCA+AGG | 0.485944 | 2.1:+39629250 | MS.gene99674:CDS |
| GATCCTAGGAGCTTCATTGA+TGG | 0.486137 | 2.1:+39630460 | MS.gene99674:CDS |
| CTTCTCTACCATTCAGAACA+AGG | 0.486214 | 2.1:+39632943 | MS.gene99674:CDS |
| ATACGCTTAACACCAAATCA+TGG | 0.492047 | 2.1:+39630818 | MS.gene99674:CDS |
| TAGATTTCAGAAGATTCAAA+AGG | 0.494537 | 2.1:+39635525 | MS.gene99674:CDS |
| GTAATTGGAATTCCGATCCT+AGG | 0.495287 | 2.1:+39630446 | MS.gene99674:CDS |
| TTATGCACCCAATAATAAAG+TGG | 0.504285 | 2.1:-39630034 | None:intergenic |
| CGTCAAAGAGAGGCATGAAA+AGG | 0.504893 | 2.1:-39630786 | None:intergenic |
| CACTCTACACCACACTGAAA+AGG | 0.505016 | 2.1:+39630750 | MS.gene99674:CDS |
| ACCGGGGCTACTTCTAAGCT+TGG | 0.505549 | 2.1:+39634936 | MS.gene99674:CDS |
| GGTTGGACTCATCACGAAGT+TGG | 0.506539 | 2.1:+39636488 | MS.gene99674:CDS |
| GTGGTTCACGCATGCCATGC+AGG | 0.511151 | 2.1:+39636443 | MS.gene99674:CDS |
| GGATGTGATATCCACTATAT+GGG | 0.514968 | 2.1:-39632886 | None:intergenic |
| GGAGTTGTACACAGTCTTGA+GGG | 0.515468 | 2.1:+39636467 | MS.gene99674:CDS |
| TTGATACCCTCAGTAGCAAA+TGG | 0.517753 | 2.1:-39633294 | None:intergenic |
| CATTGCAATGGCCCAAACTT+CGG | 0.518364 | 2.1:-39630535 | None:intergenic |
| AAGAAGAACCTTGTTCTGAA+TGG | 0.526189 | 2.1:-39632951 | None:intergenic |
| ATCAAACATGGGTTGTCCCT+AGG | 0.531029 | 2.1:+39633072 | MS.gene99674:CDS |
| ATGCCTCTCTTTGACGCACT+AGG | 0.531967 | 2.1:+39630793 | MS.gene99674:CDS |
| ATGCAGTACACAATGGATAG+AGG | 0.532071 | 2.1:+39636419 | MS.gene99674:intron |
| TGATATCCACTATATGGGCC+AGG | 0.532609 | 2.1:-39632881 | None:intergenic |
| TTGGAGGAACAACAAATTGG+TGG | 0.540504 | 2.1:-39635681 | None:intergenic |
| GTTTGCAGCGGGAACCGGTA+CGG | 0.540915 | 2.1:+39630397 | MS.gene99674:intron |
| CTCCTTTATCATCATGGCCA+TGG | 0.541676 | 2.1:+39629228 | None:intergenic |
| CAGCACAGCATTGCAAGACA+TGG | 0.541786 | 2.1:+39635599 | MS.gene99674:CDS |
| AGGAAATTGTAGTAGGGTAC+CGG | 0.549906 | 2.1:+39632848 | MS.gene99674:intron |
| GCTTAGAAGTAGCCCCGGTA+AGG | 0.550708 | 2.1:-39634932 | None:intergenic |
| TACGCTTAACACCAAATCAT+GGG | 0.553993 | 2.1:+39630819 | MS.gene99674:CDS |
| TCCTTTATCATCATGGCCAT+GGG | 0.554011 | 2.1:+39629229 | None:intergenic |
| ATCCTAGGAGCTTCATTGAT+GGG | 0.558457 | 2.1:+39630461 | MS.gene99674:CDS |
| ATCCCATCAATGAAGCTCCT+AGG | 0.559782 | 2.1:-39630463 | None:intergenic |
| ATCAATGAAGCTCCTAGGAT+CGG | 0.561148 | 2.1:-39630458 | None:intergenic |
| CACTTACCCGTTACTGACTG+TGG | 0.562180 | 2.1:-39630118 | None:intergenic |
| TCCAGTACCACAGTCAGTAA+CGG | 0.563885 | 2.1:+39630111 | MS.gene99674:CDS |
| ACATGGGTTGTCCCTAGGTG+TGG | 0.573384 | 2.1:+39633077 | MS.gene99674:CDS |
| TTGTACACAGTCTTGAGGGT+TGG | 0.575910 | 2.1:+39636471 | MS.gene99674:CDS |
| GCAGATGTTAACACTATCAA+CGG | 0.576554 | 2.1:+39635501 | MS.gene99674:intron |
| TCTGAATGGTAGAGAAGCAA+AGG | 0.579037 | 2.1:-39632937 | None:intergenic |
| GAACAATTTCAACATTGCAA+TGG | 0.582710 | 2.1:-39630547 | None:intergenic |
| AGGAGTTGTACACAGTCTTG+AGG | 0.584910 | 2.1:+39636466 | MS.gene99674:CDS |
| ACAGAATTGAGCATGCATAG+AGG | 0.586263 | 2.1:-39632907 | None:intergenic |
| TGATCACCTCACAGCAGCCA+AGG | 0.590974 | 2.1:-39635774 | None:intergenic |
| CAGGTGCTCCAAAACCGTAC+CGG | 0.593469 | 2.1:-39630411 | None:intergenic |
| TCCAAGCTTAGAAGTAGCCC+CGG | 0.602648 | 2.1:-39634937 | None:intergenic |
| GGGGTAGACTACATCAAACA+TGG | 0.605971 | 2.1:+39633060 | MS.gene99674:CDS |
| TAATTTGATGCAGTACACAA+TGG | 0.607509 | 2.1:+39636412 | MS.gene99674:intron |
| TTTCCTAGTGCGTCAAAGAG+AGG | 0.607939 | 2.1:-39630796 | None:intergenic |
| TGCAGCTATGATGTTTCATG+TGG | 0.608012 | 2.1:+39630000 | MS.gene99674:CDS |
| GAATGCAGGAAATTGTAGTA+GGG | 0.620480 | 2.1:+39632842 | MS.gene99674:intron |
| GCAGAATGAATCTCTAAATG+GGG | 0.622181 | 2.1:+39634794 | MS.gene99674:intron |
| AATATCCCAAAGTTCTCCCA+TGG | 0.622512 | 2.1:-39629245 | None:intergenic |
| AGACATGGATTGTTGGCAAG+TGG | 0.625631 | 2.1:+39635614 | MS.gene99674:CDS |
| CCACCTGAAAGCCACACCTA+GGG | 0.627760 | 2.1:-39633088 | None:intergenic |
| TGGTGTTAAAGTCATCAGCT+TGG | 0.628526 | 2.1:+39633356 | MS.gene99674:CDS |
| TGTGTACAACTCCTCCTGCA+TGG | 0.630412 | 2.1:-39636457 | None:intergenic |
| CCCACCTGAAAGCCACACCT+AGG | 0.636360 | 2.1:-39633089 | None:intergenic |
| TGGTAGCTTTGTTAGCAATG+TGG | 0.637602 | 2.1:+39632997 | MS.gene99674:CDS |
| TATGCGATTGCCAGATGATG+TGG | 0.638042 | 2.1:+39635747 | MS.gene99674:CDS |
| GAATTGAATGGTGAAGTGAA+TGG | 0.638759 | 2.1:-39630090 | None:intergenic |
| TTGATAGGATCGATGTTGTG+TGG | 0.641402 | 2.1:+39636522 | MS.gene99674:CDS |
| CATTGCTAACAAAGCTACCA+CGG | 0.641412 | 2.1:-39632994 | None:intergenic |
| GGAAGGCCTTGGCTGCTGTG+AGG | 0.644130 | 2.1:+39635768 | MS.gene99674:CDS |
| TGGAAAGCAGCACTAAAACA+TGG | 0.657991 | 2.1:+39636542 | MS.gene99674:CDS |
| TTTCAGTGTGGTGTAGAGTG+TGG | 0.670438 | 2.1:-39630747 | None:intergenic |
| CAGAATGAATCTCTAAATGG+GGG | 0.673905 | 2.1:+39634795 | MS.gene99674:intron |
| GTTCACGCATGCCATGCAGG+AGG | 0.682221 | 2.1:+39636446 | MS.gene99674:CDS |
| ACTGTGGTACTGGAATTGAA+TGG | 0.684367 | 2.1:-39630102 | None:intergenic |
| GAGCAAAATTGGGCTCCACG+TGG | 0.686831 | 2.1:+39635649 | MS.gene99674:CDS |
| GTACACAATGGATAGAGGAG+TGG | 0.689093 | 2.1:+39636424 | MS.gene99674:intron |
| AATGTTTGAAACAGACGATG+TGG | 0.700838 | 2.1:-39630569 | None:intergenic |
| GGGTAGACTACATCAAACAT+GGG | 0.706763 | 2.1:+39633061 | MS.gene99674:CDS |
| GCAGCTATGATGTTTCATGT+GGG | 0.713734 | 2.1:+39630001 | MS.gene99674:CDS |
| CGATTGCCAGATGATGTGGA+AGG | 0.713769 | 2.1:+39635751 | MS.gene99674:CDS |
| ACAGTGGAACCCGAAGACAA+AGG | 0.726600 | 2.1:-39636572 | None:intergenic |
| TTTGATGTAGTCTACCCCTG+AGG | 0.752279 | 2.1:-39633055 | None:intergenic |
| TGGTGGAAATGAGTTCCACG+TGG | 0.765678 | 2.1:-39635664 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 39629241 | 39636607 | 39629241 | ID=MS.gene99674 |
| chr2.1 | mRNA | 39629241 | 39636607 | 39629241 | ID=MS.gene99674.t1;Parent=MS.gene99674 |
| chr2.1 | exon | 39629241 | 39629271 | 39629241 | ID=MS.gene99674.t1.exon1;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39629241 | 39629271 | 39629241 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39629993 | 39630133 | 39629993 | ID=MS.gene99674.t1.exon2;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39629993 | 39630133 | 39629993 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39630405 | 39630624 | 39630405 | ID=MS.gene99674.t1.exon3;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39630405 | 39630624 | 39630405 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39630744 | 39630865 | 39630744 | ID=MS.gene99674.t1.exon4;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39630744 | 39630865 | 39630744 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39632850 | 39633106 | 39632850 | ID=MS.gene99674.t1.exon5;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39632850 | 39633106 | 39632850 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39633285 | 39633392 | 39633285 | ID=MS.gene99674.t1.exon6;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39633285 | 39633392 | 39633285 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39634798 | 39635004 | 39634798 | ID=MS.gene99674.t1.exon7;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39634798 | 39635004 | 39634798 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39635505 | 39635789 | 39635505 | ID=MS.gene99674.t1.exon8;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39635505 | 39635789 | 39635505 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| chr2.1 | exon | 39636425 | 39636607 | 39636425 | ID=MS.gene99674.t1.exon9;Parent=MS.gene99674.t1 |
| chr2.1 | CDS | 39636425 | 39636607 | 39636425 | ID=cds.MS.gene99674.t1;Parent=MS.gene99674.t1 |
| Gene Sequence |
| Protein sequence |
