AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040880.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040880.01.T01 MTR_7g100360 99.107 224 2 0 1 224 221 444 2.42e-162 456
MsG0780040880.01.T01 MTR_1g067370 92.661 218 16 0 7 224 222 439 7.01e-136 389
MsG0780040880.01.T01 MTR_3g080830 66.222 225 75 1 1 224 213 437 2.51e-106 313
MsG0780040880.01.T01 MTR_1g061930 35.537 121 75 3 106 223 1 121 4.91e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040880.01.T01 AT5G18520 86.607 224 30 0 1 224 217 440 6.99e-145 411
MsG0780040880.01.T01 AT3G09570 88.393 224 26 0 1 224 216 439 1.17e-139 398
MsG0780040880.01.T01 AT5G42090 69.778 225 67 1 1 224 215 439 2.00e-109 321
MsG0780040880.01.T01 AT5G02630 68.571 210 66 0 1 210 212 421 2.07e-106 313

Find 30 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGATTCCTCTGCAAGTTT+TGG 0.286595 7:-83808430 MsG0780040880.01.T01:CDS
GTAACCCAATCTTTGATAAA+AGG 0.306214 7:+83808372 None:intergenic
TTATCAAAGATTGGGTTACT+TGG 0.371451 7:-83808368 MsG0780040880.01.T01:CDS
AGATTGGGTTACTTGGAATC+AGG 0.404748 7:-83808361 MsG0780040880.01.T01:CDS
TTGCTGCTTATAAGTATCAA+TGG 0.424992 7:-83808137 MsG0780040880.01.T01:CDS
AAGCAGCTCAAATTGCTCTC+AGG 0.433578 7:-83808002 MsG0780040880.01.T01:CDS
AATTTAGCTTCCGTTGTGAT+TGG 0.437101 7:-83808405 MsG0780040880.01.T01:CDS
AAGCATTATGTTAAGGTCAC+TGG 0.448272 7:-83808597 MsG0780040880.01.T01:CDS
TTCCGTTGTGATTGGTGAGA+CGG 0.473034 7:-83808397 MsG0780040880.01.T01:CDS
TTTGGTCTATTAGATCACTT+AGG 0.480355 7:-83808287 MsG0780040880.01.T01:CDS
TTGGTCTATTAGATCACTTA+GGG 0.482990 7:-83808286 MsG0780040880.01.T01:CDS
GTTCTATATGTTTAGACCTG+TGG 0.484013 7:-83808064 MsG0780040880.01.T01:CDS
AGAGGATAAGCATTATGTTA+AGG 0.486485 7:-83808604 MsG0780040880.01.T01:CDS
TGCTGCTTATAAGTATCAAT+GGG 0.491550 7:-83808136 MsG0780040880.01.T01:CDS
TGTTCTTGATGAGGAGGAAG+AGG 0.503016 7:-83808025 MsG0780040880.01.T01:CDS
TCCGTTGTGATTGGTGAGAC+GGG 0.506473 7:-83808396 MsG0780040880.01.T01:CDS
AAGGTCACTGGTTTACCTCA+TGG 0.517901 7:-83808585 MsG0780040880.01.T01:CDS
AGCAGCTCAAATTGCTCTCA+GGG 0.524563 7:-83808001 MsG0780040880.01.T01:CDS
GATCTAATAGACCAAACGAT+AGG 0.528991 7:+83808294 None:intergenic
ATCTAATAGACCAAACGATA+GGG 0.534064 7:+83808295 None:intergenic
CACTGGTTTACCTCATGGAT+GGG 0.537772 7:-83808580 MsG0780040880.01.T01:CDS
TCACTGGTTTACCTCATGGA+TGG 0.546620 7:-83808581 MsG0780040880.01.T01:CDS
TGAGTACTTTGTTCTTGATG+AGG 0.557725 7:-83808034 MsG0780040880.01.T01:CDS
AAACAGATGGAAAAGCTTCG+AGG 0.570433 7:-83808254 MsG0780040880.01.T01:CDS
GTACTTTGTTCTTGATGAGG+AGG 0.589383 7:-83808031 MsG0780040880.01.T01:CDS
AGGGAAACATCGAAAACAGA+TGG 0.594318 7:-83808267 MsG0780040880.01.T01:CDS
GAATCTTATCTGTGCTGCAG+AGG 0.612979 7:-83808622 MsG0780040880.01.T01:CDS
AAATTAGCCAAAACTTGCAG+AGG 0.630803 7:+83808423 None:intergenic
ACCCGTCTCACCAATCACAA+CGG 0.703906 7:+83808395 None:intergenic
AACAATACATCCCATCCATG+AGG 0.704032 7:+83808570 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTATATTGTTGTTATT+GGG - Chr7:83808411-83808430 MsG0780040880.01.T01:CDS 15.0%
!!! CAGTTTTATATTGTTGTTAT+TGG - Chr7:83808410-83808429 MsG0780040880.01.T01:CDS 20.0%
!!! TTTACTGTTATTGTTTTGAT+CGG - Chr7:83808101-83808120 MsG0780040880.01.T01:CDS 20.0%
!!! TACATTTTTCAGTTTATTCG+TGG - Chr7:83808068-83808087 MsG0780040880.01.T01:CDS 25.0%
AGAGGATAAGCATTATGTTA+AGG - Chr7:83808010-83808029 MsG0780040880.01.T01:CDS 30.0%
ATCTAATAGACCAAACGATA+GGG + Chr7:83808322-83808341 None:intergenic 30.0%
GTAACCCAATCTTTGATAAA+AGG + Chr7:83808245-83808264 None:intergenic 30.0%
TTATCAAAGATTGGGTTACT+TGG - Chr7:83808246-83808265 MsG0780040880.01.T01:CDS 30.0%
TTGGTCTATTAGATCACTTA+GGG - Chr7:83808328-83808347 MsG0780040880.01.T01:CDS 30.0%
TTTGGTCTATTAGATCACTT+AGG - Chr7:83808327-83808346 MsG0780040880.01.T01:CDS 30.0%
! CTATTATTTTCCCTATCGTT+TGG - Chr7:83808309-83808328 MsG0780040880.01.T01:CDS 30.0%
! TGCTGCTTATAAGTATCAAT+GGG - Chr7:83808478-83808497 MsG0780040880.01.T01:CDS 30.0%
! TTGCTGCTTATAAGTATCAA+TGG - Chr7:83808477-83808496 MsG0780040880.01.T01:CDS 30.0%
! TTTTCCCTATCTTGCAAAAA+CGG + Chr7:83808149-83808168 None:intergenic 30.0%
AAATTAGCCAAAACTTGCAG+AGG + Chr7:83808194-83808213 None:intergenic 35.0%
AAGCATTATGTTAAGGTCAC+TGG - Chr7:83808017-83808036 MsG0780040880.01.T01:CDS 35.0%
GATCTAATAGACCAAACGAT+AGG + Chr7:83808323-83808342 None:intergenic 35.0%
GTTCTATATGTTTAGACCTG+TGG - Chr7:83808550-83808569 MsG0780040880.01.T01:CDS 35.0%
TGAGTACTTTGTTCTTGATG+AGG - Chr7:83808580-83808599 MsG0780040880.01.T01:CDS 35.0%
! AATTTAGCTTCCGTTGTGAT+TGG - Chr7:83808209-83808228 MsG0780040880.01.T01:CDS 35.0%
! GGGTCCTTTTATCAAAGATT+GGG - Chr7:83808238-83808257 MsG0780040880.01.T01:CDS 35.0%
!! AAGTACTCATTTTTCTCCAC+AGG + Chr7:83808569-83808588 None:intergenic 35.0%
!! GAAACCGTTTTTGCAAGATA+GGG - Chr7:83808142-83808161 MsG0780040880.01.T01:CDS 35.0%
!! TGAAACCGTTTTTGCAAGAT+AGG - Chr7:83808141-83808160 MsG0780040880.01.T01:CDS 35.0%
!!! GTTATTGTTTTGATCGGTAC+TGG - Chr7:83808107-83808126 MsG0780040880.01.T01:CDS 35.0%
AAACAGATGGAAAAGCTTCG+AGG - Chr7:83808360-83808379 MsG0780040880.01.T01:CDS 40.0%
AACAATACATCCCATCCATG+AGG + Chr7:83808047-83808066 None:intergenic 40.0%
AGATTGGGTTACTTGGAATC+AGG - Chr7:83808253-83808272 MsG0780040880.01.T01:CDS 40.0%
AGGGAAACATCGAAAACAGA+TGG - Chr7:83808347-83808366 MsG0780040880.01.T01:CDS 40.0%
GTACTTTGTTCTTGATGAGG+AGG - Chr7:83808583-83808602 MsG0780040880.01.T01:CDS 40.0%
! CGGGTCCTTTTATCAAAGAT+TGG - Chr7:83808237-83808256 MsG0780040880.01.T01:CDS 40.0%
! TGTGATTCCTCTGCAAGTTT+TGG - Chr7:83808184-83808203 MsG0780040880.01.T01:CDS 40.0%
!!! TTGTTTTGATCGGTACTGGT+TGG - Chr7:83808111-83808130 MsG0780040880.01.T01:CDS 40.0%
AAGCAGCTCAAATTGCTCTC+AGG - Chr7:83808612-83808631 MsG0780040880.01.T01:CDS 45.0%
AGCAGCTCAAATTGCTCTCA+GGG - Chr7:83808613-83808632 MsG0780040880.01.T01:CDS 45.0%
GAATCTTATCTGTGCTGCAG+AGG - Chr7:83807992-83808011 MsG0780040880.01.T01:CDS 45.0%
TGTTCTTGATGAGGAGGAAG+AGG - Chr7:83808589-83808608 MsG0780040880.01.T01:CDS 45.0%
TTCCGTTGTGATTGGTGAGA+CGG - Chr7:83808217-83808236 MsG0780040880.01.T01:CDS 45.0%
!! AAGGTCACTGGTTTACCTCA+TGG - Chr7:83808029-83808048 MsG0780040880.01.T01:CDS 45.0%
!! CACTGGTTTACCTCATGGAT+GGG - Chr7:83808034-83808053 MsG0780040880.01.T01:CDS 45.0%
!! TCACTGGTTTACCTCATGGA+TGG - Chr7:83808033-83808052 MsG0780040880.01.T01:CDS 45.0%
ACCCGTCTCACCAATCACAA+CGG + Chr7:83808222-83808241 None:intergenic 50.0%
TCCGTTGTGATTGGTGAGAC+GGG - Chr7:83808218-83808237 MsG0780040880.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83807981 83808655 83807981 ID=MsG0780040880.01;Name=MsG0780040880.01
Chr7 mRNA 83807981 83808655 83807981 ID=MsG0780040880.01.T01;Parent=MsG0780040880.01;Name=MsG0780040880.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|224
Chr7 exon 83807981 83808655 83807981 ID=MsG0780040880.01.T01:exon:19922;Parent=MsG0780040880.01.T01
Chr7 CDS 83807981 83808655 83807981 ID=MsG0780040880.01.T01:cds;Parent=MsG0780040880.01.T01
Gene Sequence

>MsG0780040880.01.T01

ATGAAGGCTTTGAATCTTATCTGTGCTGCAGAGGATAAGCATTATGTTAAGGTCACTGGTTTACCTCATGGATGGGATGTATTGTTCTACATTTTTCAGTTTATTCGTGGTGTTGTGTTGTTTACTGTTATTGTTTTGATCGGTACTGGTTGGTCGTTTCTGAAACCGTTTTTGCAAGATAGGGAAAAGAAAGTGTTGATGATTGTGATTCCTCTGCAAGTTTTGGCTAATTTAGCTTCCGTTGTGATTGGTGAGACGGGTCCTTTTATCAAAGATTGGGTTACTTGGAATCAGGTGTTTTTGCTTGTTGATATTATTTGTTGTTGTGCTATTATTTTCCCTATCGTTTGGTCTATTAGATCACTTAGGGAAACATCGAAAACAGATGGAAAAGCTTCGAGGAATCTTGCGAAATTGACACTTTTTAGACAGTTTTATATTGTTGTTATTGGGTATCTGTATTTTACAAGAATTGTTGTGTTTGCTTTGAGAACTATTGCTGCTTATAAGTATCAATGGGTGAGTAATGCTGCTGAAGAGACTGCTAGTCTTGCTTTCTATATCATTATGTTCTATATGTTTAGACCTGTGGAGAAAAATGAGTACTTTGTTCTTGATGAGGAGGAAGAGGAAGCAGCTCAAATTGCTCTCAGGGATGAAGAATTTGAGCTTTAA

Protein sequence

>MsG0780040880.01.T01

MKALNLICAAEDKHYVKVTGLPHGWDVLFYIFQFIRGVVLFTVIVLIGTGWSFLKPFLQDREKKVLMIVIPLQVLANLASVVIGETGPFIKDWVTWNQVFLLVDIICCCAIIFPIVWSIRSLRETSKTDGKASRNLAKLTLFRQFYIVVIGYLYFTRIVVFALRTIAAYKYQWVSNAAEETASLAFYIIMFYMFRPVEKNEYFVLDEEEEEAAQIALRDEEFEL*