AlfalfaGEDB | MsG0280007551.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007551.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007551.01.T01	ABN08857.1	97.945	146	3	0	1	146	1	146	4.71E-98	288

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007551.01.T01	A2Q5F7	97.945	146	3	0	1	146	1	146	2.25e-98	288

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047912.01	MsG0280007551.01	0.820796	5.675534e-53	4.535610e-50
MsG0080048322.01	MsG0280007551.01	0.806162	9.404520e-50	5.068874e-47
MsG0080048843.01	MsG0280007551.01	0.810021	1.417729e-50	8.453525e-48
MsG0180000271.01	MsG0280007551.01	0.807181	5.728975e-50	3.169756e-47
MsG0180000284.01	MsG0280007551.01	0.811476	6.870629e-51	4.257270e-48
MsG0180000680.01	MsG0280007551.01	0.801692	7.985169e-49	3.837884e-46
MsG0180000806.01	MsG0280007551.01	0.819145	1.354155e-52	1.033571e-49
MsG0180002955.01	MsG0280007551.01	0.800959	1.128431e-48	5.324430e-46
MsG0180003379.01	MsG0280007551.01	0.816046	6.765293e-52	4.742944e-49
MsG0180004160.01	MsG0280007551.01	0.804108	2.530972e-49	1.293948e-46
MsG0180004272.01	MsG0280007551.01	0.805944	1.045206e-49	5.602191e-47
MsG0180005106.01	MsG0280007551.01	0.802910	4.482643e-49	2.222447e-46
MsG0180005304.01	MsG0280007551.01	0.809087	2.250299e-50	1.308905e-47
MsG0180005445.01	MsG0280007551.01	0.802897	4.510475e-49	2.235496e-46
MsG0180005765.01	MsG0280007551.01	0.813005	3.185859e-51	2.056178e-48
MsG0280006679.01	MsG0280007551.01	0.807051	6.104494e-50	3.366055e-47
MsG0280006728.01	MsG0280007551.01	0.815938	7.151568e-52	4.998563e-49
MsG0280006906.01	MsG0280007551.01	0.806519	7.908918e-50	4.301660e-47
MsG0280006935.01	MsG0280007551.01	0.805643	1.209302e-49	6.431628e-47
MsG0280007109.01	MsG0280007551.01	0.837355	5.460111e-57	7.073799e-54
MsG0280007153.01	MsG0280007551.01	0.809522	1.814922e-50	1.067987e-47
MsG0280007551.01	MsG0280007585.01	0.811261	7.646733e-51	4.711323e-48
MsG0280007551.01	MsG0280007645.01	0.814603	1.416611e-51	9.547807e-49
MsG0280007551.01	MsG0280007745.01	0.819171	1.335977e-52	1.020456e-49
MsG0280007551.01	MsG0280007775.01	0.822013	2.972549e-53	2.457884e-50
MsG0280007551.01	MsG0280008286.01	0.805640	1.210670e-49	6.438508e-47
MsG0280007551.01	MsG0280008502.01	0.812247	4.667766e-51	2.952733e-48
MsG0280007551.01	MsG0280008970.01	0.829270	5.653211e-55	5.758369e-52
MsG0280007551.01	MsG0280008971.01	0.803883	2.817650e-49	1.432292e-46
MsG0280007551.01	MsG0280010158.01	0.806800	6.898982e-50	3.779485e-47
MsG0280007551.01	MsG0280010389.01	0.803684	3.098936e-49	1.567147e-46
MsG0280007551.01	MsG0280010531.01	0.821618	3.668858e-53	2.999786e-50
MsG0280007551.01	MsG0280010976.01	0.814506	1.487807e-51	1.000217e-48
MsG0280007551.01	MsG0280011070.01	0.802241	6.158330e-49	3.001594e-46
MsG0280007551.01	MsG0280011235.01	0.811909	5.530560e-51	3.466249e-48
MsG0280007551.01	MsG0280011334.01	0.801203	1.005720e-48	4.774620e-46
MsG0280007551.01	MsG0380011729.01	0.821572	3.760332e-53	3.070727e-50
MsG0280007551.01	MsG0380012020.01	0.824955	6.096785e-54	5.481569e-51
MsG0280007551.01	MsG0380012057.01	0.800669	1.293126e-48	6.056612e-46
MsG0280007551.01	MsG0380012458.01	0.804962	1.679314e-49	8.776920e-47
MsG0280007551.01	MsG0380014576.01	0.802822	4.674101e-49	2.312294e-46
MsG0280007551.01	MsG0380014880.01	0.800403	1.465010e-48	6.815555e-46
MsG0280007551.01	MsG0380015289.01	0.818357	2.044769e-52	1.527258e-49
MsG0280007551.01	MsG0380015614.01	0.803584	3.251347e-49	1.640125e-46
MsG0280007551.01	MsG0380016055.01	0.804660	1.941999e-49	1.007045e-46
MsG0280007551.01	MsG0380017003.01	0.806823	6.821730e-50	3.739409e-47
MsG0280007551.01	MsG0380017210.01	0.816603	5.078769e-52	3.615027e-49
MsG0280007551.01	MsG0380017226.01	0.822269	2.593359e-53	2.160214e-50
MsG0280007551.01	MsG0380017403.01	0.807435	5.062966e-50	2.819712e-47
MsG0280007551.01	MsG0380017512.01	0.804365	2.237383e-49	1.151448e-46
MsG0280007551.01	MsG0480019404.01	0.806258	8.978342e-50	4.851028e-47
MsG0280007551.01	MsG0480020331.01	0.801399	9.172009e-49	4.375745e-46
MsG0280007551.01	MsG0480020416.01	0.816080	6.648994e-52	4.665692e-49
MsG0280007551.01	MsG0480020719.01	0.808786	2.609974e-50	1.505888e-47
MsG0280007551.01	MsG0480020720.01	0.800597	1.337298e-48	6.252005e-46
MsG0280007551.01	MsG0480020843.01	0.802104	6.571604e-49	3.191762e-46
MsG0280007551.01	MsG0480021896.01	-0.801684	8.017659e-49	3.852666e-46
MsG0280007551.01	MsG0480022026.01	0.803339	3.655147e-49	1.832054e-46
MsG0280007551.01	MsG0480022104.01	0.801154	1.029611e-48	4.882063e-46
MsG0280007551.01	MsG0480022108.01	0.806294	8.820896e-50	4.770214e-47
MsG0280007551.01	MsG0480022115.01	0.802974	4.347641e-49	2.158882e-46
MsG0280007551.01	MsG0480022467.01	0.810418	1.164277e-50	7.014148e-48
MsG0280007551.01	MsG0480024014.01	0.805884	1.075921e-49	5.757951e-47
MsG0280007551.01	MsG0580025278.01	0.815017	1.146844e-51	7.816367e-49
MsG0280007551.01	MsG0580025453.01	0.809473	1.859473e-50	1.092760e-47
MsG0280007551.01	MsG0580025520.01	0.822943	1.806825e-53	1.534206e-50
MsG0280007551.01	MsG0580025769.01	0.802262	6.097518e-49	2.973519e-46
MsG0280007551.01	MsG0580025986.01	0.801538	8.590400e-49	4.112764e-46
MsG0280007551.01	MsG0580026225.01	0.804966	1.675905e-49	8.759947e-47
MsG0280007551.01	MsG0580028289.01	0.804926	1.708901e-49	8.923448e-47
MsG0280007551.01	MsG0580028526.01	0.811714	6.098531e-51	3.802471e-48
MsG0280007551.01	MsG0580029258.01	0.810622	1.051783e-50	6.371285e-48
MsG0280007551.01	MsG0580029628.01	0.832706	8.109921e-56	9.137711e-53
MsG0280007551.01	MsG0580029658.01	0.819698	1.013098e-52	7.852860e-50
MsG0280007551.01	MsG0680030841.01	0.804658	1.943522e-49	1.007781e-46
MsG0280007551.01	MsG0680031133.01	0.817600	3.031696e-52	2.217597e-49
MsG0280007551.01	MsG0680031145.01	0.804153	2.476621e-49	1.267643e-46
MsG0280007551.01	MsG0680031301.01	0.827408	1.590140e-54	1.533976e-51
MsG0280007551.01	MsG0680031654.01	0.844953	5.493572e-59	9.014459e-56
MsG0280007551.01	MsG0680035048.01	0.817249	3.637257e-52	2.635084e-49
MsG0280007551.01	MsG0680035601.01	0.809486	1.848202e-50	1.086474e-47
MsG0280007551.01	MsG0780036117.01	0.809462	1.870252e-50	1.098755e-47
MsG0280007551.01	MsG0780036633.01	0.816373	5.718863e-52	4.045093e-49
MsG0280007551.01	MsG0780036635.01	0.806372	8.495211e-50	4.603147e-47
MsG0280007551.01	MsG0780036810.01	0.809222	2.104742e-50	1.228698e-47
MsG0280007551.01	MsG0780036845.01	0.811233	7.756104e-51	4.775209e-48
MsG0280007551.01	MsG0780038636.01	0.806458	8.145455e-50	4.423573e-47
MsG0280007551.01	MsG0780039570.01	0.807772	4.293507e-50	2.412270e-47
MsG0280007551.01	MsG0780039777.01	0.801377	9.265047e-49	4.417896e-46
MsG0280007551.01	MsG0780040110.01	0.802641	5.094359e-49	2.508364e-46
MsG0280007551.01	MsG0780040176.01	0.800514	1.390626e-48	6.487621e-46
MsG0280007551.01	MsG0780040182.01	0.829493	4.992063e-55	5.117338e-52
MsG0280007551.01	MsG0780040208.01	0.837515	4.966368e-57	6.467163e-54
MsG0280007551.01	MsG0780040424.01	0.820437	6.861991e-53	5.429529e-50
MsG0280007551.01	MsG0780040742.01	0.800536	1.376460e-48	6.424997e-46
MsG0280007551.01	MsG0780041127.01	0.806800	6.896944e-50	3.778429e-47
MsG0280007551.01	MsG0780041161.01	0.807584	4.706489e-50	2.631255e-47
MsG0280007551.01	MsG0780041399.01	0.800723	1.260681e-48	5.912563e-46
MsG0280007551.01	MsG0780041498.01	0.805193	1.502645e-49	7.899774e-47
MsG0280007551.01	MsG0880041969.01	0.812483	4.143955e-51	2.638076e-48
MsG0280007551.01	MsG0880042206.01	0.802887	4.532372e-49	2.245749e-46
MsG0280007551.01	MsG0880042792.01	0.810070	1.383740e-50	8.260967e-48
MsG0280007551.01	MsG0880043082.01	0.805497	1.297148e-49	6.872929e-47
MsG0280007551.01	MsG0880043162.01	0.808502	3.001568e-50	1.719121e-47
MsG0280007551.01	MsG0880043194.01	0.827770	1.301971e-54	1.269080e-51
MsG0280007551.01	MsG0880043625.01	0.805372	1.377760e-49	7.276788e-47
MsG0280007551.01	MsG0880043804.01	0.808935	2.425469e-50	1.404970e-47
MsG0280007551.01	MsG0880044062.01	-0.806817	6.841513e-50	3.749766e-47
MsG0280007551.01	MsG0880046047.01	0.804139	2.493572e-49	1.275851e-46
MsG0280007551.01	MsG0880046068.01	0.800406	1.463194e-48	6.807587e-46
MsG0280007551.01	MsG0880046244.01	0.804921	1.712779e-49	8.942674e-47
MsG0280007551.01	MsG0880046450.01	0.817383	3.392806e-52	2.467234e-49
MsG0280007551.01	MsG0880046561.01	0.823941	1.056247e-53	9.225166e-51
MsG0280007551.01	MsG0880047391.01	0.863980	1.689548e-64	5.219775e-61
MsG0280007551.01	MsG0880047590.01	0.801846	7.424421e-49	3.582560e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007551.01.T01	MTR_2g027700	97.945	146	3	0	1	146	1	146	5.71e-102	288

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGCTTGAAGCAGAAGATT+TGG	0.301631	2:+17037380	MsG0280007551.01.T01:CDS
GAGAAAGGTTGTTTAGCATT+TGG	0.326273	2:+17037138	MsG0280007551.01.T01:CDS
GCCATTTGAGGTTAATAGAT+TGG	0.344754	2:+17037359	MsG0280007551.01.T01:CDS
CCAAGACAACACTTGTTTCA+AGG	0.366636	2:+17037257	MsG0280007551.01.T01:CDS
AAATCCTCCATTGATCCCTT+TGG	0.386929	2:-17037330	None:intergenic
TTCTGGTATTGATTTCTTTG+AGG	0.395501	2:+17037284	MsG0280007551.01.T01:CDS
ACTTGTTTCAAGGTTTCTTC+TGG	0.404656	2:+17037267	MsG0280007551.01.T01:CDS
TCCAATCTATTAACCTCAAA+TGG	0.414962	2:-17037360	None:intergenic
CCTTGAAACAAGTGTTGTCT+TGG	0.422034	2:-17037257	None:intergenic
TTGGAGTGTTTGATTTCCCT+TGG	0.432978	2:+17037157	MsG0280007551.01.T01:CDS
ATGATGTTGAAGCAAAGATT+TGG	0.461294	2:+17037031	MsG0280007551.01.T01:CDS
AGGCTTAATTGTTTAGCAAA+GGG	0.461500	2:-17037416	None:intergenic
AGGGATCAATGGAGGATTTG+TGG	0.464367	2:+17037334	MsG0280007551.01.T01:CDS
GGATTTGTGGCTGCCATTTG+AGG	0.474098	2:+17037347	MsG0280007551.01.T01:CDS
GATTTCCCTTGGTTGAAAGA+TGG	0.486271	2:+17037168	MsG0280007551.01.T01:CDS
GAGGCTTAATTGTTTAGCAA+AGG	0.489138	2:-17037417	None:intergenic
TGAGGCTCCAAAGGGATCAA+TGG	0.510645	2:+17037323	MsG0280007551.01.T01:CDS
AAGAATGAAGTGGAAGAGAA+AGG	0.519180	2:+17037123	MsG0280007551.01.T01:CDS
TAGATTGTGTGAGGCTCCAA+AGG	0.564320	2:+17037314	MsG0280007551.01.T01:CDS
AGATTGTGTGAGGCTCCAAA+GGG	0.596314	2:+17037315	MsG0280007551.01.T01:CDS
CACCAAGGAAAAGAATGAAG+TGG	0.603149	2:+17037113	MsG0280007551.01.T01:CDS
TGACACCATCTTTCAACCAA+GGG	0.626669	2:-17037173	None:intergenic
ATGACACCATCTTTCAACCA+AGG	0.654692	2:-17037174	None:intergenic
GGCTCCAAAGGGATCAATGG+AGG	0.655913	2:+17037326	MsG0280007551.01.T01:CDS
GGAAAATGATAGATTGTGTG+AGG	0.713281	2:+17037305	MsG0280007551.01.T01:CDS
ATGTGGCACAAGTTTCACCA+AGG	0.735233	2:+17037098	MsG0280007551.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGATGTTGAAGCAAAGATT+TGG	+	Chr2:17037031-17037050	MsG0280007551.01.T01:CDS	30.0%
	GCTAAAAAAAGAGATGTATG+TGG	+	Chr2:17037081-17037100	MsG0280007551.01.T01:CDS	30.0%
	TCCAATCTATTAACCTCAAA+TGG	-	Chr2:17037363-17037382	None:intergenic	30.0%
!!	TTCTGGTATTGATTTCTTTG+AGG	+	Chr2:17037284-17037303	MsG0280007551.01.T01:CDS	30.0%
	AAGAATGAAGTGGAAGAGAA+AGG	+	Chr2:17037123-17037142	MsG0280007551.01.T01:CDS	35.0%
	ACTTGTTTCAAGGTTTCTTC+TGG	+	Chr2:17037267-17037286	MsG0280007551.01.T01:CDS	35.0%
	GAGAAAGGTTGTTTAGCATT+TGG	+	Chr2:17037138-17037157	MsG0280007551.01.T01:CDS	35.0%
	GGAAAATGATAGATTGTGTG+AGG	+	Chr2:17037305-17037324	MsG0280007551.01.T01:CDS	35.0%
!	GCCATTTGAGGTTAATAGAT+TGG	+	Chr2:17037359-17037378	MsG0280007551.01.T01:CDS	35.0%
!	TTCCACTTCATTCTTTTCCT+TGG	-	Chr2:17037118-17037137	None:intergenic	35.0%
	AAATCCTCCATTGATCCCTT+TGG	-	Chr2:17037333-17037352	None:intergenic	40.0%
	ATGACACCATCTTTCAACCA+AGG	-	Chr2:17037177-17037196	None:intergenic	40.0%
	CACCAAGGAAAAGAATGAAG+TGG	+	Chr2:17037113-17037132	MsG0280007551.01.T01:CDS	40.0%
	CCAAGACAACACTTGTTTCA+AGG	+	Chr2:17037257-17037276	MsG0280007551.01.T01:CDS	40.0%
	GATTTCCCTTGGTTGAAAGA+TGG	+	Chr2:17037168-17037187	MsG0280007551.01.T01:CDS	40.0%
	TGACACCATCTTTCAACCAA+GGG	-	Chr2:17037176-17037195	None:intergenic	40.0%
!	CCTTGAAACAAGTGTTGTCT+TGG	-	Chr2:17037260-17037279	None:intergenic	40.0%
!	TTGGAGTGTTTGATTTCCCT+TGG	+	Chr2:17037157-17037176	MsG0280007551.01.T01:CDS	40.0%
	AGATTGTGTGAGGCTCCAAA+GGG	+	Chr2:17037315-17037334	MsG0280007551.01.T01:CDS	45.0%
	AGGGATCAATGGAGGATTTG+TGG	+	Chr2:17037334-17037353	MsG0280007551.01.T01:CDS	45.0%
	ATGTGGCACAAGTTTCACCA+AGG	+	Chr2:17037098-17037117	MsG0280007551.01.T01:CDS	45.0%
	GGAGCTTGAAGCAGAAGATT+TGG	+	Chr2:17037380-17037399	MsG0280007551.01.T01:CDS	45.0%
	TAGATTGTGTGAGGCTCCAA+AGG	+	Chr2:17037314-17037333	MsG0280007551.01.T01:CDS	45.0%
	TGAGGCTCCAAAGGGATCAA+TGG	+	Chr2:17037323-17037342	MsG0280007551.01.T01:CDS	50.0%
!	GGATTTGTGGCTGCCATTTG+AGG	+	Chr2:17037347-17037366	MsG0280007551.01.T01:CDS	50.0%
	GGCTCCAAAGGGATCAATGG+AGG	+	Chr2:17037326-17037345	MsG0280007551.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	17036994	17037434	17036994	ID=MsG0280007551.01;Name=MsG0280007551.01
Chr2	mRNA	17036994	17037434	17036994	ID=MsG0280007551.01.T01;Parent=MsG0280007551.01;Name=MsG0280007551.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|146
Chr2	exon	17036994	17037434	17036994	ID=MsG0280007551.01.T01:exon:3664;Parent=MsG0280007551.01.T01
Chr2	CDS	17036994	17037434	17036994	ID=MsG0280007551.01.T01:cds;Parent=MsG0280007551.01.T01

Gene Sequence

>MsG0280007551.01.T01

ATGGAGCAAGAGAAGAATAAGAGAGCAAGAGAATATGATGATGTTGAAGCAAAGATTTGGTTTTCTTTTGAGAATAATAACAAGAAAGCTAAAAAAAGAGATGTATGTGGCACAAGTTTCACCAAGGAAAAGAATGAAGTGGAAGAGAAAGGTTGTTTAGCATTTGGAGTGTTTGATTTCCCTTGGTTGAAAGATGGTGTCATATACAAATTAGAAGATTGTTTAATTGATTTTGAAGACAATTTCTTGTCATCATTACAAGACCAAGACAACACTTGTTTCAAGGTTTCTTCTGGTATTGATTTCTTTGAGGAAAATGATAGATTGTGTGAGGCTCCAAAGGGATCAATGGAGGATTTGTGGCTGCCATTTGAGGTTAATAGATTGGAGCTTGAAGCAGAAGATTTGGATTGTATTTGTAGCCCTTTGCTAAACAATTAA

Protein sequence

>MsG0280007551.01.T01

MEQEKNKRAREYDDVEAKIWFSFENNNKKAKKRDVCGTSFTKEKNEVEEKGCLAFGVFDFPWLKDGVIYKLEDCLIDFEDNFLSSLQDQDNTCFKVSSGIDFFEENDRLCEAPKGSMEDLWLPFEVNRLELEAEDLDCICSPLLNN*