AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041127.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041127.01.T01 MTR_7g104610 98.039 153 3 0 1 153 1 153 2.01e-107 303
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041127.01.T01 AT5G15770 71.918 146 41 0 7 152 3 148 3.26e-74 219

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTTGCAATAATCTTTC+CGG 0.199227 7:-86899206 None:intergenic
GTCTGTAATCTCTAATCTTC+TGG 0.252175 7:-86899044 None:intergenic
TCGTTCTGTTGGGTGTTATA+AGG 0.300594 7:+86899360 MsG0780041127.01.T01:CDS
TAAGAAATTGTGGTAAAGTT+GGG 0.348212 7:+86899254 MsG0780041127.01.T01:CDS
TTAAGAAATTGTGGTAAAGT+TGG 0.366943 7:+86899253 MsG0780041127.01.T01:CDS
ACCGATCATGCTCGTTCTGT+TGG 0.371791 7:+86899349 MsG0780041127.01.T01:CDS
AGTGCTCGTGGGAAACAATT+GGG 0.376890 7:+86899304 MsG0780041127.01.T01:CDS
ACCGGAAAGATTATTGCAAC+GGG 0.406061 7:+86899205 MsG0780041127.01.T01:CDS
GTTATTGAAGATGAGATTAC+CGG 0.417687 7:+86899187 MsG0780041127.01.T01:CDS
AAGTGCTCGTGGGAAACAAT+TGG 0.427736 7:+86899303 MsG0780041127.01.T01:CDS
CCGATCATGCTCGTTCTGTT+GGG 0.443895 7:+86899350 MsG0780041127.01.T01:CDS
AGTCTCTGATAAGGAATTTG+AGG 0.451854 7:+86899117 MsG0780041127.01.T01:CDS
TTGTGATTCAGTCTCTGATA+AGG 0.468883 7:+86899108 MsG0780041127.01.T01:CDS
TTTCGAGAGGTTGATTCACT+TGG 0.471380 7:+86899145 MsG0780041127.01.T01:CDS
CGATTGCAGTGTTGAGAACA+AGG 0.494534 7:+86899390 MsG0780041127.01.T01:CDS
CTATGAGAAATGTGGTTTCA+AGG 0.496011 7:+86899417 MsG0780041127.01.T01:CDS
CCCAACAGAACGAGCATGAT+CGG 0.532044 7:-86899350 None:intergenic
TACCGGAAAGATTATTGCAA+CGG 0.546187 7:+86899204 MsG0780041127.01.T01:CDS
CAAGGAGAAAGAAGTTCAGA+TGG 0.566779 7:+86899435 MsG0780041127.01.T01:CDS
GTTGTGGATTCAAGTGCTCG+TGG 0.610212 7:+86899292 MsG0780041127.01.T01:CDS
ATTTGAGGATCGATTTCGAG+AGG 0.616403 7:+86899132 MsG0780041127.01.T01:CDS
GCATATAGAAGATGTTGTTG+TGG 0.621571 7:+86899276 MsG0780041127.01.T01:CDS
GTGCTCGTGGGAAACAATTG+GGG 0.624260 7:+86899305 MsG0780041127.01.T01:CDS
TTGTGGATTCAAGTGCTCGT+GGG 0.642750 7:+86899293 MsG0780041127.01.T01:CDS
AAGGTGTTCTATGAGAAATG+TGG 0.671486 7:+86899409 MsG0780041127.01.T01:CDS
TTAGAAGATGCAAAACAGTG+AGG 0.719583 7:+86899006 None:intergenic
CCGGAAAGATTATTGCAACG+GGG 0.727047 7:+86899206 MsG0780041127.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATTTTTAAGAAATTG+TGG + Chr7:86899244-86899263 MsG0780041127.01.T01:CDS 15.0%
! TAAGAAATTGTGGTAAAGTT+GGG + Chr7:86899254-86899273 MsG0780041127.01.T01:CDS 25.0%
! TTAAGAAATTGTGGTAAAGT+TGG + Chr7:86899253-86899272 MsG0780041127.01.T01:CDS 25.0%
GAGATTACAGACAAGAAAAA+AGG + Chr7:86899055-86899074 MsG0780041127.01.T01:CDS 30.0%
GTTATTGAAGATGAGATTAC+CGG + Chr7:86899187-86899206 MsG0780041127.01.T01:CDS 30.0%
AGTCTCTGATAAGGAATTTG+AGG + Chr7:86899117-86899136 MsG0780041127.01.T01:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG + Chr7:86899417-86899436 MsG0780041127.01.T01:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG + Chr7:86899276-86899295 MsG0780041127.01.T01:CDS 35.0%
GTCTGTAATCTCTAATCTTC+TGG - Chr7:86899047-86899066 None:intergenic 35.0%
TACCGGAAAGATTATTGCAA+CGG + Chr7:86899204-86899223 MsG0780041127.01.T01:CDS 35.0%
TTGTGATTCAGTCTCTGATA+AGG + Chr7:86899108-86899127 MsG0780041127.01.T01:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG + Chr7:86899409-86899428 MsG0780041127.01.T01:CDS 35.0%
ACCGGAAAGATTATTGCAAC+GGG + Chr7:86899205-86899224 MsG0780041127.01.T01:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG + Chr7:86899435-86899454 MsG0780041127.01.T01:CDS 40.0%
! ATTTGAGGATCGATTTCGAG+AGG + Chr7:86899132-86899151 MsG0780041127.01.T01:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG + Chr7:86899360-86899379 MsG0780041127.01.T01:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG + Chr7:86899145-86899164 MsG0780041127.01.T01:CDS 40.0%
AAGTGCTCGTGGGAAACAAT+TGG + Chr7:86899303-86899322 MsG0780041127.01.T01:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG + Chr7:86899304-86899323 MsG0780041127.01.T01:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG - Chr7:86899209-86899228 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG + Chr7:86899206-86899225 MsG0780041127.01.T01:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG + Chr7:86899293-86899312 MsG0780041127.01.T01:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG + Chr7:86899390-86899409 MsG0780041127.01.T01:CDS 45.0%
ACCGATCATGCTCGTTCTGT+TGG + Chr7:86899349-86899368 MsG0780041127.01.T01:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG - Chr7:86899353-86899372 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG + Chr7:86899350-86899369 MsG0780041127.01.T01:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG + Chr7:86899305-86899324 MsG0780041127.01.T01:CDS 50.0%
GTTGTGGATTCAAGTGCTCG+TGG + Chr7:86899292-86899311 MsG0780041127.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86899013 86899474 86899013 ID=MsG0780041127.01;Name=MsG0780041127.01
Chr7 mRNA 86899013 86899474 86899013 ID=MsG0780041127.01.T01;Parent=MsG0780041127.01;Name=MsG0780041127.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|153
Chr7 exon 86899013 86899474 86899013 ID=MsG0780041127.01.T01:exon:11963;Parent=MsG0780041127.01.T01
Chr7 CDS 86899013 86899474 86899013 ID=MsG0780041127.01.T01:cds;Parent=MsG0780041127.01.T01
Gene Sequence

>MsG0780041127.01.T01

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTACCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATGTATTTTGTTTGA

Protein sequence

>MsG0780041127.01.T01

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEITGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAMYFV*