AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007645.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007645.01.T01 MTR_7g094120 86.164 159 21 1 1 158 1 159 1.98e-98 280
MsG0280007645.01.T01 MTR_7g021400 77.987 159 31 3 1 158 1 156 8.43e-79 231
MsG0280007645.01.T01 MTR_7g094280 71.875 160 41 3 1 158 1 158 1.90e-77 227
MsG0280007645.01.T01 MTR_7g094150 76.730 159 33 3 1 158 1 156 5.02e-77 226
MsG0280007645.01.T01 MTR_7g094170 75.472 159 35 3 1 158 1 156 2.51e-74 219
MsG0280007645.01.T01 MTR_5g022710 74.843 159 35 3 1 158 1 155 3.41e-74 219
MsG0280007645.01.T01 MTR_5g022730 73.585 159 37 3 1 158 1 155 5.75e-73 216
MsG0280007645.01.T01 MTR_3g110048 68.553 159 48 2 1 158 1 158 5.81e-72 213
MsG0280007645.01.T01 MTR_4g032450 67.516 157 47 3 4 158 21 175 3.78e-71 212
MsG0280007645.01.T01 MTR_7g021380 73.585 159 38 3 1 158 1 156 1.85e-70 209
MsG0280007645.01.T01 MTR_4g032400 66.667 159 49 3 2 158 7 163 1.96e-68 205
MsG0280007645.01.T01 MTR_7g094310 66.875 160 39 4 1 158 1 148 5.69e-68 203
MsG0280007645.01.T01 MTR_8g056060 65.625 160 50 4 1 158 1 157 3.22e-65 196
MsG0280007645.01.T01 MTR_4g032510 62.733 161 49 4 1 158 1 153 1.44e-63 192
MsG0280007645.01.T01 MTR_8g468480 63.125 160 53 4 1 158 1 156 6.09e-61 186
MsG0280007645.01.T01 MTR_4g032500 59.375 160 55 3 1 158 1 152 6.11e-61 185
MsG0280007645.01.T01 MTR_4g032460 62.500 160 48 4 1 158 1 150 1.75e-58 179
MsG0280007645.01.T01 MTR_4g033455 58.599 157 61 3 3 158 7 160 1.36e-57 177
MsG0280007645.01.T01 MTR_5g085030 59.119 159 59 4 1 157 1 155 4.16e-57 176
MsG0280007645.01.T01 MTR_4g017860 51.031 194 53 4 7 158 27 220 4.87e-54 170
MsG0280007645.01.T01 MTR_4g033460 59.441 143 54 3 3 144 7 146 7.60e-51 163
MsG0280007645.01.T01 MTR_4g033460 60.000 95 27 3 42 135 161 245 4.39e-27 102
MsG0280007645.01.T01 MTR_5g082340 55.000 160 55 4 1 158 1 145 2.58e-47 150
MsG0280007645.01.T01 MTR_1g099870 71.845 103 25 2 58 158 3 103 7.91e-47 148
MsG0280007645.01.T01 MTR_7g094320 50.633 158 37 1 1 158 1 117 2.77e-45 144
MsG0280007645.01.T01 MTR_4g021390 52.532 158 69 3 2 158 3 155 2.94e-45 145
MsG0280007645.01.T01 MTR_8g089730 61.600 125 28 2 35 158 1 106 4.58e-43 138
MsG0280007645.01.T01 MTR_7g094410 51.553 161 28 3 1 116 1 156 7.82e-40 132
MsG0280007645.01.T01 MTR_4g032420 52.713 129 43 4 32 158 38 150 4.01e-38 127
MsG0280007645.01.T01 MTR_7g094360 46.243 173 40 4 1 121 1 172 6.59e-37 126
MsG0280007645.01.T01 MTR_4g032470 47.260 146 30 3 15 158 34 134 2.36e-35 120
MsG0280007645.01.T01 MTR_4g131980 54.348 138 49 6 1 135 1 127 3.58e-35 120
MsG0280007645.01.T01 MTR_4g127240 60.952 105 32 5 41 142 26 124 4.03e-32 111
MsG0280007645.01.T01 MTR_7g006370 47.917 144 63 4 2 144 3 135 3.16e-31 109
MsG0280007645.01.T01 MTR_7g094350 41.579 190 55 7 1 137 1 187 6.82e-29 105
MsG0280007645.01.T01 MTR_8g056090 47.170 159 47 9 1 156 1 125 8.00e-28 100
MsG0280007645.01.T01 MTR_4g119422 40.136 147 78 3 3 146 227 366 8.94e-24 95.5
MsG0280007645.01.T01 MTR_2g066010 37.107 159 85 5 3 157 14 161 1.06e-23 92.0
MsG0280007645.01.T01 MTR_2g065720 37.107 159 85 5 3 157 31 178 3.92e-23 90.9
MsG0280007645.01.T01 MTR_4g032520 44.628 121 39 3 31 149 2 96 6.18e-23 88.6
MsG0280007645.01.T01 MTR_1g112590 34.839 155 80 5 11 147 3 154 3.46e-22 87.0
MsG0280007645.01.T01 MTR_7g071200 59.420 69 27 1 91 158 4 72 1.72e-21 82.8
MsG0280007645.01.T01 MTR_2g018980 35.714 154 79 5 9 158 3 140 2.79e-20 82.0
MsG0280007645.01.T01 MTR_5g096950 33.544 158 88 4 1 157 1 142 9.30e-19 78.2
MsG0280007645.01.T01 MTR_4g032540 57.143 63 26 1 92 153 70 132 2.52e-18 76.6
MsG0280007645.01.T01 MTR_5g078680 30.323 155 67 4 11 147 3 134 2.07e-16 74.3
MsG0280007645.01.T01 MTR_1g112570 33.594 128 60 4 9 118 1 121 1.35e-15 72.4
MsG0280007645.01.T01 MTR_1g042510 30.573 157 68 5 9 147 1 134 9.61e-15 70.1
MsG0280007645.01.T01 MTR_8g007185 31.469 143 82 5 11 145 13 147 9.42e-14 67.4
MsG0280007645.01.T01 MTR_8g007195 33.803 142 79 5 11 145 13 146 1.17e-13 67.4
MsG0280007645.01.T01 MTR_8g007205 31.469 143 82 5 11 145 13 147 1.58e-13 66.6
MsG0280007645.01.T01 MTR_6g053620 32.237 152 88 5 1 145 9 152 3.87e-13 65.9
MsG0280007645.01.T01 MTR_6g053620 32.667 150 86 5 3 145 11 152 4.98e-13 65.9
MsG0280007645.01.T01 MTR_2g100830 29.730 148 89 3 6 146 8 147 1.09e-12 64.7
MsG0280007645.01.T01 MTR_2g100825 30.612 147 88 4 5 145 7 145 1.92e-12 63.9
MsG0280007645.01.T01 MTR_8g030670 34.343 99 57 2 48 145 62 153 1.61e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007645.01.T01 AT1G20140 68.125 160 45 2 5 158 4 163 3.68e-73 217
MsG0280007645.01.T01 AT2G25700 66.250 160 48 2 5 158 4 163 3.72e-69 206
MsG0280007645.01.T01 AT1G75950 68.553 159 45 2 5 158 2 160 1.33e-68 205
MsG0280007645.01.T01 AT4G34210 62.581 155 53 2 5 158 2 152 1.52e-65 197
MsG0280007645.01.T01 AT5G42190 62.353 170 49 2 4 158 2 171 3.38e-65 197
MsG0280007645.01.T01 AT4G34470 60.645 155 56 2 5 158 2 152 7.61e-63 190
MsG0280007645.01.T01 AT3G60010 59.355 155 57 3 7 158 3 154 1.27e-58 179
MsG0280007645.01.T01 AT2G03190 55.556 162 60 3 9 158 6 167 3.70e-55 171
MsG0280007645.01.T01 AT3G21860 58.333 156 59 5 4 158 2 152 4.56e-55 171
MsG0280007645.01.T01 AT2G03170 57.237 152 55 3 9 158 6 149 1.92e-54 169
MsG0280007645.01.T01 AT3G21850 54.777 157 64 4 4 158 2 153 3.39e-52 163
MsG0280007645.01.T01 AT3G25650 55.556 162 60 5 9 158 6 167 4.71e-49 156
MsG0280007645.01.T01 AT2G03160 48.705 193 61 6 1 158 1 190 1.50e-48 155
MsG0280007645.01.T01 AT2G20160 52.258 155 65 3 5 157 2 149 2.40e-47 151
MsG0280007645.01.T01 AT3G60020 50.318 157 63 5 9 158 5 153 6.74e-46 147
MsG0280007645.01.T01 AT1G10230 52.980 151 69 2 9 157 6 156 1.23e-45 147
MsG0280007645.01.T01 AT3G21830 49.359 156 73 3 4 158 2 152 1.18e-43 141
MsG0280007645.01.T01 AT3G21840 50.413 121 54 5 4 123 2 117 1.87e-27 99.8
MsG0280007645.01.T01 AT3G53060 56.977 86 27 4 32 115 3 80 1.91e-22 85.9
MsG0280007645.01.T01 AT2G45950 31.544 149 89 5 4 146 13 154 2.87e-13 66.2
MsG0280007645.01.T01 AT2G45950 31.544 149 89 5 4 146 13 154 2.87e-13 66.2
MsG0280007645.01.T01 AT2G45950 31.757 148 88 5 4 145 13 153 3.17e-13 66.2
MsG0280007645.01.T01 AT2G45950 31.757 148 88 5 4 145 13 153 3.17e-13 66.2
MsG0280007645.01.T01 AT2G45950 31.757 148 88 5 4 145 13 153 3.17e-13 66.2
MsG0280007645.01.T01 AT3G61415 31.293 147 86 5 6 145 15 153 4.48e-12 63.2
MsG0280007645.01.T01 AT3G61415 31.293 147 86 5 6 145 15 153 4.77e-12 62.8

Find 42 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAGGGTGATCATCTTCT+TGG 0.232916 2:-18482222 None:intergenic
CAGAAGATTATCTTAAGAAT+TGG 0.268289 2:+18482438 MsG0280007645.01.T01:CDS
TTTCACCGTCGAAACTCTTC+AGG 0.296550 2:-18482238 None:intergenic
AACCTTCGCTAATACCTTGC+CGG 0.357004 2:-18482360 None:intergenic
TGCCGGTAACGTTCTGAATC+GGG 0.375308 2:-18482343 None:intergenic
CATGTCCGCCGCAGCTTGGC+AGG 0.378684 2:-18482552 None:intergenic
CTGAATCGGGATTGCGTTGT+CGG 0.392692 2:-18482330 None:intergenic
AGAAGATTATCTTAAGAATT+GGG 0.396941 2:+18482439 MsG0280007645.01.T01:CDS
ATCCCGATTCAGAACGTTAC+CGG 0.408425 2:+18482341 MsG0280007645.01.T01:CDS
CATCCTTGCTGCAAACTACT+TGG 0.414458 2:+18482505 MsG0280007645.01.T01:CDS
CGAACTGCTTCCTCTTCCTC+CGG 0.424372 2:-18482635 None:intergenic
TTGCCGGTAACGTTCTGAAT+CGG 0.445008 2:-18482344 None:intergenic
CTTGGATATCAAGAGTCTAC+TGG 0.463162 2:+18482523 MsG0280007645.01.T01:CDS
TCATCATGTCCGCCGCAGCT+TGG 0.472634 2:-18482556 None:intergenic
TGATTTCGTGAAGGTTGATA+AGG 0.476483 2:+18482466 MsG0280007645.01.T01:CDS
TGATTACACTCCGGAGGAAG+AGG 0.478345 2:+18482625 MsG0280007645.01.T01:CDS
CTTCGAGGTTTCCGAGGCTG+TGG 0.487029 2:+18482262 MsG0280007645.01.T01:CDS
CTTCTTGGTTGTTGAAGACA+TGG 0.497969 2:-18482207 None:intergenic
TGATTCAAGCGCCACAGCCT+CGG 0.536432 2:-18482273 None:intergenic
AGATAATCTTCTGATTCTGA+AGG 0.536670 2:-18482428 None:intergenic
TCTTACCTGCCAAGCTGCGG+CGG 0.548433 2:+18482547 MsG0280007645.01.T01:CDS
TTCACCGTCGAAACTCTTCA+GGG 0.553987 2:-18482237 None:intergenic
GGAAGAGGAAGCAGTTCGAA+AGG 0.554040 2:+18482640 MsG0280007645.01.T01:CDS
TTTCGACGGTGAAACCTTCG+AGG 0.563465 2:+18482247 MsG0280007645.01.T01:CDS
CAGTTCGAAAGGAAAACGCA+TGG 0.575446 2:+18482651 MsG0280007645.01.T01:CDS
GATTCAGAACGTTACCGGCA+AGG 0.604913 2:+18482346 MsG0280007645.01.T01:CDS
TACCGGCAAGGTATTAGCGA+AGG 0.608061 2:+18482358 MsG0280007645.01.T01:CDS
ATCACCCTGAAGAGTTTCGA+CGG 0.609201 2:+18482233 MsG0280007645.01.T01:CDS
CACAGCCTCGGAAACCTCGA+AGG 0.613440 2:-18482261 None:intergenic
GACAATCAAGCACATGATCG+AGG 0.614315 2:+18482298 MsG0280007645.01.T01:CDS
TGAAACCTTCGAGGTTTCCG+AGG 0.616843 2:+18482256 MsG0280007645.01.T01:CDS
GCTGCGGCGGACATGATGAA+AGG 0.621251 2:+18482560 MsG0280007645.01.T01:CDS
AGTTCGAAAGGAAAACGCAT+GGG 0.640914 2:+18482652 MsG0280007645.01.T01:CDS
CTGCAAGAAGCACGTCGAGG+TGG 0.646155 2:+18482388 MsG0280007645.01.T01:CDS
CATGATGAAAGGAAAGCAAG+TGG 0.648020 2:+18482571 MsG0280007645.01.T01:CDS
TATCCAAGTAGTTTGCAGCA+AGG 0.660134 2:-18482508 None:intergenic
GGATCTTACCTGCCAAGCTG+CGG 0.660372 2:+18482544 MsG0280007645.01.T01:CDS
TTGGGATGCTGATTTCGTGA+AGG 0.669657 2:+18482457 MsG0280007645.01.T01:CDS
CAAGAATGATTACACTCCGG+AGG 0.672447 2:+18482619 MsG0280007645.01.T01:CDS
CATCAAGAATGATTACACTC+CGG 0.688170 2:+18482616 MsG0280007645.01.T01:CDS
GATGAAAGGAAAGCAAGTGG+AGG 0.701585 2:+18482574 MsG0280007645.01.T01:CDS
ATACTGCAAGAAGCACGTCG+AGG 0.756219 2:+18482385 MsG0280007645.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATTATCTTAAGAATT+GGG + Chr2:18482439-18482458 MsG0280007645.01.T01:CDS 20.0%
! CAGAAGATTATCTTAAGAAT+TGG + Chr2:18482438-18482457 MsG0280007645.01.T01:CDS 25.0%
AGATAATCTTCTGATTCTGA+AGG - Chr2:18482431-18482450 None:intergenic 30.0%
!! TGATTCTGAAGGTTTTTCAT+CGG - Chr2:18482420-18482439 None:intergenic 30.0%
CATCAAGAATGATTACACTC+CGG + Chr2:18482616-18482635 MsG0280007645.01.T01:CDS 35.0%
!! TGATTTCGTGAAGGTTGATA+AGG + Chr2:18482466-18482485 MsG0280007645.01.T01:CDS 35.0%
AGTTCGAAAGGAAAACGCAT+GGG + Chr2:18482652-18482671 MsG0280007645.01.T01:CDS 40.0%
CATGATGAAAGGAAAGCAAG+TGG + Chr2:18482571-18482590 MsG0280007645.01.T01:CDS 40.0%
CTTGGATATCAAGAGTCTAC+TGG + Chr2:18482523-18482542 MsG0280007645.01.T01:CDS 40.0%
GCAAGGATGAGTTCAAAAAG+CGG - Chr2:18482494-18482513 None:intergenic 40.0%
! TATCCAAGTAGTTTGCAGCA+AGG - Chr2:18482511-18482530 None:intergenic 40.0%
AACCTTCGCTAATACCTTGC+CGG - Chr2:18482363-18482382 None:intergenic 45.0%
ATCACCCTGAAGAGTTTCGA+CGG + Chr2:18482233-18482252 MsG0280007645.01.T01:CDS 45.0%
ATCCCGATTCAGAACGTTAC+CGG + Chr2:18482341-18482360 MsG0280007645.01.T01:CDS 45.0%
CAAGAATGATTACACTCCGG+AGG + Chr2:18482619-18482638 MsG0280007645.01.T01:CDS 45.0%
CAGTTCGAAAGGAAAACGCA+TGG + Chr2:18482651-18482670 MsG0280007645.01.T01:CDS 45.0%
CATCCTTGCTGCAAACTACT+TGG + Chr2:18482505-18482524 MsG0280007645.01.T01:CDS 45.0%
CTTCAGGGTGATCATCTTCT+TGG - Chr2:18482225-18482244 None:intergenic 45.0%
GACAATCAAGCACATGATCG+AGG + Chr2:18482298-18482317 MsG0280007645.01.T01:CDS 45.0%
GATGAAAGGAAAGCAAGTGG+AGG + Chr2:18482574-18482593 MsG0280007645.01.T01:CDS 45.0%
TTCACCGTCGAAACTCTTCA+GGG - Chr2:18482240-18482259 None:intergenic 45.0%
TTTCACCGTCGAAACTCTTC+AGG - Chr2:18482241-18482260 None:intergenic 45.0%
! TTGCCGGTAACGTTCTGAAT+CGG - Chr2:18482347-18482366 None:intergenic 45.0%
!! TTGGGATGCTGATTTCGTGA+AGG + Chr2:18482457-18482476 MsG0280007645.01.T01:CDS 45.0%
CTGAATCGGGATTGCGTTGT+CGG - Chr2:18482333-18482352 None:intergenic 50.0%
GATTCAGAACGTTACCGGCA+AGG + Chr2:18482346-18482365 MsG0280007645.01.T01:CDS 50.0%
GGAAGAGGAAGCAGTTCGAA+AGG + Chr2:18482640-18482659 MsG0280007645.01.T01:CDS 50.0%
TGAAACCTTCGAGGTTTCCG+AGG + Chr2:18482256-18482275 MsG0280007645.01.T01:CDS 50.0%
TGATTACACTCCGGAGGAAG+AGG + Chr2:18482625-18482644 MsG0280007645.01.T01:CDS 50.0%
TTTCGACGGTGAAACCTTCG+AGG + Chr2:18482247-18482266 MsG0280007645.01.T01:CDS 50.0%
! ATACTGCAAGAAGCACGTCG+AGG + Chr2:18482385-18482404 MsG0280007645.01.T01:CDS 50.0%
! TACCGGCAAGGTATTAGCGA+AGG + Chr2:18482358-18482377 MsG0280007645.01.T01:CDS 50.0%
! TGCCGGTAACGTTCTGAATC+GGG - Chr2:18482346-18482365 None:intergenic 50.0%
CGAACTGCTTCCTCTTCCTC+CGG - Chr2:18482638-18482657 None:intergenic 55.0%
GGATCTTACCTGCCAAGCTG+CGG + Chr2:18482544-18482563 MsG0280007645.01.T01:CDS 55.0%
TGATTCAAGCGCCACAGCCT+CGG - Chr2:18482276-18482295 None:intergenic 55.0%
CACAGCCTCGGAAACCTCGA+AGG - Chr2:18482264-18482283 None:intergenic 60.0%
CTTCGAGGTTTCCGAGGCTG+TGG + Chr2:18482262-18482281 MsG0280007645.01.T01:CDS 60.0%
GCTGCGGCGGACATGATGAA+AGG + Chr2:18482560-18482579 MsG0280007645.01.T01:CDS 60.0%
TCATCATGTCCGCCGCAGCT+TGG - Chr2:18482559-18482578 None:intergenic 60.0%
TCTTACCTGCCAAGCTGCGG+CGG + Chr2:18482547-18482566 MsG0280007645.01.T01:CDS 60.0%
! CTGCAAGAAGCACGTCGAGG+TGG + Chr2:18482388-18482407 MsG0280007645.01.T01:CDS 60.0%
CATGTCCGCCGCAGCTTGGC+AGG - Chr2:18482555-18482574 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 18482209 18482685 18482209 ID=MsG0280007645.01;Name=MsG0280007645.01
Chr2 mRNA 18482209 18482685 18482209 ID=MsG0280007645.01.T01;Parent=MsG0280007645.01;Name=MsG0280007645.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|158
Chr2 exon 18482209 18482685 18482209 ID=MsG0280007645.01.T01:exon:9958;Parent=MsG0280007645.01.T01
Chr2 CDS 18482209 18482685 18482209 ID=MsG0280007645.01.T01:cds;Parent=MsG0280007645.01.T01
Gene Sequence

>MsG0280007645.01.T01

ATGTCTTCAACAACCAAGAAGATGATCACCCTGAAGAGTTTCGACGGTGAAACCTTCGAGGTTTCCGAGGCTGTGGCGCTTGAATCACAGACAATCAAGCACATGATCGAGGATGATTGTGCCGACAACGCAATCCCGATTCAGAACGTTACCGGCAAGGTATTAGCGAAGGTTGAATACTGCAAGAAGCACGTCGAGGTGGCGAGTGCCGATGAAAAACCTTCAGAATCAGAAGATTATCTTAAGAATTGGGATGCTGATTTCGTGAAGGTTGATAAGGAACCGCTTTTTGAACTCATCCTTGCTGCAAACTACTTGGATATCAAGAGTCTACTGGATCTTACCTGCCAAGCTGCGGCGGACATGATGAAAGGAAAGCAAGTGGAGGAAGTTCGCACGATGTTTAACATCAAGAATGATTACACTCCGGAGGAAGAGGAAGCAGTTCGAAAGGAAAACGCATGGGCATTTGAATGA

Protein sequence

>MsG0280007645.01.T01

MSSTTKKMITLKSFDGETFEVSEAVALESQTIKHMIEDDCADNAIPIQNVTGKVLAKVEYCKKHVEVASADEKPSESEDYLKNWDADFVKVDKEPLFELILAANYLDIKSLLDLTCQAAADMMKGKQVEEVRTMFNIKNDYTPEEEEAVRKENAWAFE*