AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045401.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045401.01.T01 MTR_8g070890 85.965 57 7 1 65 120 13 69 2.80e-25 96.3
MsG0880045401.01.T01 MTR_8g070880 68.750 80 23 2 42 120 65 143 5.83e-24 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045401.01.T01 AT1G79620 50.633 79 39 0 42 120 73 151 7.50e-16 72.8
MsG0880045401.01.T01 AT1G79620 50.633 79 39 0 42 120 73 151 7.74e-16 72.8
MsG0880045401.01.T01 AT1G79620 50.633 79 39 0 42 120 73 151 9.88e-16 72.4
MsG0880045401.01.T01 AT1G79620 50.633 79 39 0 42 120 73 151 1.02e-15 72.4
MsG0880045401.01.T01 AT1G79620 50.633 79 39 0 42 120 73 151 1.39e-15 72.0

Find 20 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAATTCGATATACTTTA+AGG 0.082582 8:-58770926 MsG0880045401.01.T01:CDS
AATTAACCGTTTCAGGATTT+TGG 0.091828 8:-58770116 MsG0880045401.01.T01:intron
GTCCTTTAATAAAGGTTTAA+TGG 0.162551 8:-58770264 MsG0880045401.01.T01:CDS
ACCCATTAAACCTTTATTAA+AGG 0.167669 8:+58770262 None:intergenic
TTGGATGGAAATTGTTACTA+TGG 0.311539 8:-58770757 MsG0880045401.01.T01:CDS
TCCTTTAATAAAGGTTTAAT+GGG 0.312324 8:-58770263 MsG0880045401.01.T01:CDS
TTGCTTAAGTCTCCCAGTTC+TGG 0.319541 8:+58770230 None:intergenic
ATCTGCTGTTAGGCGACTTT+GGG 0.325585 8:-58770806 MsG0880045401.01.T01:CDS
AGGGACTTGTCCTTTAATAA+AGG 0.349948 8:-58770272 MsG0880045401.01.T01:CDS
GGGTTCTATCTCTCCAGAAC+TGG 0.382542 8:-58770243 MsG0880045401.01.T01:CDS
TATCTGCTGTTAGGCGACTT+TGG 0.398697 8:-58770807 MsG0880045401.01.T01:CDS
TGGCAATATTCCAGATAGAT+TGG 0.406263 8:-58770077 MsG0880045401.01.T01:CDS
GGCAATATTCCAGATAGATT+GGG 0.408938 8:-58770076 MsG0880045401.01.T01:CDS
CAGAATGTATATCTGCTGTT+AGG 0.468497 8:-58770816 MsG0880045401.01.T01:CDS
TTGGCTGGTTGCAGCTTCAG+TGG 0.503624 8:-58770097 MsG0880045401.01.T01:CDS
ATTCGATATACTTTAAGGAC+TGG 0.556252 8:-58770921 MsG0880045401.01.T01:CDS
GGTTCTATCTCTCCAGAACT+GGG 0.574112 8:-58770242 MsG0880045401.01.T01:CDS
GCAATATTCCAGATAGATTG+GGG 0.585469 8:-58770075 MsG0880045401.01.T01:CDS
TGGCTACCGAACAAATGCAC+AGG 0.624953 8:-58770901 MsG0880045401.01.T01:CDS
CAATATTCCAGATAGATTGG+GGG 0.728079 8:-58770074 MsG0880045401.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAGACTAAAT+TGG - Chr8:58770475-58770494 MsG0880045401.01.T01:intron 15.0%
!!! AACTTTTAGTAATTTTCATA+AGG - Chr8:58770819-58770838 MsG0880045401.01.T01:CDS 15.0%
!!! TCTCTTTTTTTTTTTCTTTT+CGG + Chr8:58770469-58770488 None:intergenic 15.0%
!! TAATATGCAAATACATTGTA+TGG + Chr8:58770648-58770667 None:intergenic 20.0%
!! TCCTTTAATAAAGGTTTAAT+GGG - Chr8:58770701-58770720 MsG0880045401.01.T01:intron 20.0%
!!! AAGTATTGTAATTGTGATTA+TGG + Chr8:58770559-58770578 None:intergenic 20.0%
!!! AGATTTTGTTTTAAATAGAG+GGG - Chr8:58770305-58770324 MsG0880045401.01.T01:intron 20.0%
!!! CAGATTTTGTTTTAAATAGA+GGG - Chr8:58770304-58770323 MsG0880045401.01.T01:intron 20.0%
!!! TTTTATGACTAAAACTAAGT+TGG + Chr8:58770606-58770625 None:intergenic 20.0%
! ACCCATTAAACCTTTATTAA+AGG + Chr8:58770705-58770724 None:intergenic 25.0%
! GACAAATTCGATATACTTTA+AGG - Chr8:58770038-58770057 MsG0880045401.01.T01:CDS 25.0%
! GTCCTTTAATAAAGGTTTAA+TGG - Chr8:58770700-58770719 MsG0880045401.01.T01:intron 25.0%
!! GATTTAAAATTAACCGTTTC+AGG - Chr8:58770841-58770860 MsG0880045401.01.T01:CDS 25.0%
!!! CTAAAACTAAGTTGGTATTA+TGG + Chr8:58770598-58770617 None:intergenic 25.0%
!!! GATTTTGTTTTAAATAGAGG+GGG - Chr8:58770306-58770325 MsG0880045401.01.T01:intron 25.0%
!!! GCAGATTTTGTTTTAAATAG+AGG - Chr8:58770303-58770322 MsG0880045401.01.T01:intron 25.0%
AAAAATCCTGTGCATTTGTT+CGG + Chr8:58770072-58770091 None:intergenic 30.0%
AATGACATTGGGAAATTAAC+AGG + Chr8:58770791-58770810 None:intergenic 30.0%
AGTTTCACTTGAAATGACAT+TGG + Chr8:58770803-58770822 None:intergenic 30.0%
GTTTCACTTGAAATGACATT+GGG + Chr8:58770802-58770821 None:intergenic 30.0%
TTGGATGGAAATTGTTACTA+TGG - Chr8:58770207-58770226 MsG0880045401.01.T01:intron 30.0%
! AATTAACCGTTTCAGGATTT+TGG - Chr8:58770848-58770867 MsG0880045401.01.T01:CDS 30.0%
! ATTCGATATACTTTAAGGAC+TGG - Chr8:58770043-58770062 MsG0880045401.01.T01:CDS 30.0%
!!! TGCAATTTGTTTTGGATTGA+TGG - Chr8:58770368-58770387 MsG0880045401.01.T01:intron 30.0%
!!! TTTTCTGGTTTTTAGTAGCA+GGG - Chr8:58770263-58770282 MsG0880045401.01.T01:CDS 30.0%
AGGGACTTGTCCTTTAATAA+AGG - Chr8:58770692-58770711 MsG0880045401.01.T01:intron 35.0%
CAAACCACATTACAACTTTC+AGG + Chr8:58770408-58770427 None:intergenic 35.0%
CAATATTCCAGATAGATTGG+GGG - Chr8:58770890-58770909 MsG0880045401.01.T01:CDS 35.0%
CAGAATGTATATCTGCTGTT+AGG - Chr8:58770148-58770167 MsG0880045401.01.T01:intron 35.0%
CTATATTGTAAACCACTGCA+CGG + Chr8:58770334-58770353 None:intergenic 35.0%
CTGAAAGTTGTAATGTGGTT+TGG - Chr8:58770406-58770425 MsG0880045401.01.T01:intron 35.0%
GCAATATTCCAGATAGATTG+GGG - Chr8:58770889-58770908 MsG0880045401.01.T01:CDS 35.0%
GGCAATATTCCAGATAGATT+GGG - Chr8:58770888-58770907 MsG0880045401.01.T01:CDS 35.0%
GTATAGAGTAAACTAGCTCA+TGG + Chr8:58770447-58770466 None:intergenic 35.0%
TGGCAATATTCCAGATAGAT+TGG - Chr8:58770887-58770906 MsG0880045401.01.T01:CDS 35.0%
TGTCAATTCATGTAACTGCA+GGG - Chr8:58770673-58770692 MsG0880045401.01.T01:intron 35.0%
TGTTCCTGAAAGTTGTAATG+TGG - Chr8:58770401-58770420 MsG0880045401.01.T01:intron 35.0%
! AATTGTTTGCTGCAGAATGT+AGG - Chr8:58770230-58770249 MsG0880045401.01.T01:CDS 35.0%
! CCATCCAAAGCAATTTTTGA+CGG + Chr8:58770195-58770214 None:intergenic 35.0%
! GAACAAATGCACAGGATTTT+TGG - Chr8:58770071-58770090 MsG0880045401.01.T01:CDS 35.0%
!!! GTAGGAGACTTTTAGTTTTC+TGG - Chr8:58770248-58770267 MsG0880045401.01.T01:CDS 35.0%
!!! GTTTTCTGGTTTTTAGTAGC+AGG - Chr8:58770262-58770281 MsG0880045401.01.T01:CDS 35.0%
!!! TTTCTGGTTTTTAGTAGCAG+GGG - Chr8:58770264-58770283 MsG0880045401.01.T01:CDS 35.0%
AACCAGCCAAAATCCTGAAA+CGG + Chr8:58770857-58770876 None:intergenic 40.0%
CAAAACAAATTGCACACGAG+TGG + Chr8:58770362-58770381 None:intergenic 40.0%
CATGCCGTCAAAAATTGCTT+TGG - Chr8:58770188-58770207 MsG0880045401.01.T01:intron 40.0%
GTGTCAATTCATGTAACTGC+AGG - Chr8:58770672-58770691 MsG0880045401.01.T01:intron 40.0%
! AGTTGTAATGTGGTTTGGAG+CGG - Chr8:58770411-58770430 MsG0880045401.01.T01:intron 40.0%
! CAAAGCAATTTTTGACGGCA+TGG + Chr8:58770190-58770209 None:intergenic 40.0%
! CCGTCAAAAATTGCTTTGGA+TGG - Chr8:58770192-58770211 MsG0880045401.01.T01:intron 40.0%
!! CACTCGTGTGCAATTTGTTT+TGG - Chr8:58770360-58770379 MsG0880045401.01.T01:intron 40.0%
!!! AATGCACAGGATTTTTGGTC+AGG - Chr8:58770076-58770095 MsG0880045401.01.T01:CDS 40.0%
!!! ATGCACAGGATTTTTGGTCA+GGG - Chr8:58770077-58770096 MsG0880045401.01.T01:CDS 40.0%
!!! TTCTGGTTTTTAGTAGCAGG+GGG - Chr8:58770265-58770284 MsG0880045401.01.T01:CDS 40.0%
ATCTGCTGTTAGGCGACTTT+GGG - Chr8:58770158-58770177 MsG0880045401.01.T01:intron 45.0%
GAAAGATCCCCCAATCTATC+TGG + Chr8:58770900-58770919 None:intergenic 45.0%
GGTTCTATCTCTCCAGAACT+GGG - Chr8:58770722-58770741 MsG0880045401.01.T01:intron 45.0%
TATCTGCTGTTAGGCGACTT+TGG - Chr8:58770157-58770176 MsG0880045401.01.T01:intron 45.0%
TTGCTTAAGTCTCCCAGTTC+TGG + Chr8:58770737-58770756 None:intergenic 45.0%
! AGCAATTTTTGACGGCATGG+AGG + Chr8:58770187-58770206 None:intergenic 45.0%
! CTGCTACTGCAGAATTTTGC+AGG + Chr8:58770125-58770144 None:intergenic 45.0%
!! AACCGTTTCAGGATTTTGGC+TGG - Chr8:58770852-58770871 MsG0880045401.01.T01:CDS 45.0%
!!! TCTGGTTTTTAGTAGCAGGG+GGG - Chr8:58770266-58770285 MsG0880045401.01.T01:CDS 45.0%
GGGTTCTATCTCTCCAGAAC+TGG - Chr8:58770721-58770740 MsG0880045401.01.T01:intron 50.0%
TGGCTACCGAACAAATGCAC+AGG - Chr8:58770063-58770082 MsG0880045401.01.T01:CDS 50.0%
!!! CTGGTTTTTAGTAGCAGGGG+GGG - Chr8:58770267-58770286 MsG0880045401.01.T01:CDS 50.0%
! TTGGCTGGTTGCAGCTTCAG+TGG - Chr8:58770867-58770886 MsG0880045401.01.T01:CDS 55.0%
AGTAGCAGGGGGGGATGCAC+TGG - Chr8:58770276-58770295 MsG0880045401.01.T01:intron 65.0%
ATAGAGGGGGAGCCGTGCAG+TGG - Chr8:58770319-58770338 MsG0880045401.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 58770036 58770950 58770036 ID=MsG0880045401.01;Name=MsG0880045401.01
Chr8 mRNA 58770036 58770950 58770036 ID=MsG0880045401.01.T01;Parent=MsG0880045401.01;Name=MsG0880045401.01.T01;_AED=0.50;_eAED=0.80;_QI=0|0|0|1|0.5|0.66|3|0|124
Chr8 exon 58770739 58770950 58770739 ID=MsG0880045401.01.T01:exon:22627;Parent=MsG0880045401.01.T01
Chr8 exon 58770218 58770292 58770218 ID=MsG0880045401.01.T01:exon:22626;Parent=MsG0880045401.01.T01
Chr8 exon 58770036 58770123 58770036 ID=MsG0880045401.01.T01:exon:22625;Parent=MsG0880045401.01.T01
Chr8 CDS 58770739 58770950 58770739 ID=MsG0880045401.01.T01:cds;Parent=MsG0880045401.01.T01
Chr8 CDS 58770218 58770292 58770218 ID=MsG0880045401.01.T01:cds;Parent=MsG0880045401.01.T01
Chr8 CDS 58770036 58770123 58770036 ID=MsG0880045401.01.T01:cds;Parent=MsG0880045401.01.T01
Gene Sequence

>MsG0880045401.01.T01

ATGACAAATTCGATATACTTTAAGGACTGGCTACCGAACAAATGCACAGGATTTTTGGTCAGGGTCTGTTATGAATTGTTGCAAAGCCTGCAAAATTCTGCAGTAGCAGATGCAGAATGTATATCTGCTGTTAGGCGACTTTGGGTTACCTCCATGCCGTCAAAAATTGCTTTGGATGGAAATTGTTACTATGGAATTGTTTGCTGCAGAATGGACTTGTCCTTTAATAAAGGTTTAATGGGTTCTATCTCTCCAGAACTGGGAGACTTAAGCAAGCTAAATATTCTGATTTTGGCTGGTTGCAGCTTCAGTGGCAATATTCCAGATAGATTGGGGGATCTTTCAGAGCTATCTTTCTTGTATGAATTTTTTTAG

Protein sequence

>MsG0880045401.01.T01

MTNSIYFKDWLPNKCTGFLVRVCYELLQSLQNSAVADAECISAVRRLWVTSMPSKIALDGNCYYGIVCCRMDLSFNKGLMGSISPELGDLSKLNILILAGCSFSGNIPDRLGDLSELSFLYEFF*