AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045031.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045031.01.T01 MTR_4g037265 80.612 98 12 1 1 91 60 157 4.76e-50 156
MsG0880045031.01.T01 MTR_8g028545 94.643 56 3 0 25 80 113 168 3.91e-34 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045031.01.T01 AT2G46100 75.862 58 14 0 25 82 119 176 1.94e-27 100
MsG0880045031.01.T01 AT2G46100 76.786 56 13 0 25 80 119 174 5.07e-26 96.7

Find 16 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTAACTTTGGATCACTTC+TGG 0.287804 8:+52990589 MsG0880045031.01.T01:CDS
AAAAGGAACTGCACTAACTT+TGG 0.371780 8:+52990578 MsG0880045031.01.T01:CDS
TGTGAGATCTCCTGAGAGTT+GGG 0.440744 8:-52990486 None:intergenic
AAGCAGTAGAAAAGTGTTCA+AGG 0.443650 8:-52990663 None:intergenic
ATCTATTCCTCCCAACTCTC+AGG 0.462430 8:+52990476 MsG0880045031.01.T01:CDS
TGAAGAGGATTGTGAATTTG+CGG 0.489339 8:+52990520 MsG0880045031.01.T01:CDS
AAGGACTTCAGCGTTTCAAA+AGG 0.491123 8:+52990561 MsG0880045031.01.T01:CDS
CAACTATGAATCTTATGAAG+TGG 0.531678 8:+52990618 MsG0880045031.01.T01:CDS
TGTGAATTTGCGGATCCAGC+GGG 0.538988 8:+52990530 MsG0880045031.01.T01:CDS
CACATTGAATGCATATGAAG+AGG 0.540073 8:+52990505 MsG0880045031.01.T01:CDS
AACTATGAATCTTATGAAGT+GGG 0.541276 8:+52990619 MsG0880045031.01.T01:CDS
ATGTGAGATCTCCTGAGAGT+TGG 0.544846 8:-52990487 None:intergenic
AGTCCTTTAAAAGATCCCGC+TGG 0.610274 8:-52990545 None:intergenic
TTGTGAATTTGCGGATCCAG+CGG 0.612444 8:+52990529 MsG0880045031.01.T01:CDS
TTATAATCAGATGGTTCACA+TGG 0.613230 8:+52990450 MsG0880045031.01.T01:intron
GAGATCTCCTGAGAGTTGGG+AGG 0.695820 8:-52990483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTTGATTATAATCAGA+TGG + Chr8:52990441-52990460 MsG0880045031.01.T01:intron 20.0%
!!! TTTTGTATCAGTCATTATAT+AGG - Chr8:52990390-52990409 None:intergenic 20.0%
! AACTATGAATCTTATGAAGT+GGG + Chr8:52990619-52990638 MsG0880045031.01.T01:CDS 25.0%
! CAAAATGAAGCTAATACAAT+TGG + Chr8:52990405-52990424 MsG0880045031.01.T01:intron 25.0%
CAACTATGAATCTTATGAAG+TGG + Chr8:52990618-52990637 MsG0880045031.01.T01:CDS 30.0%
TTATAATCAGATGGTTCACA+TGG + Chr8:52990450-52990469 MsG0880045031.01.T01:intron 30.0%
AAAAGGAACTGCACTAACTT+TGG + Chr8:52990578-52990597 MsG0880045031.01.T01:CDS 35.0%
CACATTGAATGCATATGAAG+AGG + Chr8:52990505-52990524 MsG0880045031.01.T01:CDS 35.0%
TGAAGAGGATTGTGAATTTG+CGG + Chr8:52990520-52990539 MsG0880045031.01.T01:CDS 35.0%
! TATGAAGTGGGAAGATTTTG+AGG + Chr8:52990631-52990650 MsG0880045031.01.T01:CDS 35.0%
!! AAGCAGTAGAAAAGTGTTCA+AGG - Chr8:52990666-52990685 None:intergenic 35.0%
AAGGACTTCAGCGTTTCAAA+AGG + Chr8:52990561-52990580 MsG0880045031.01.T01:CDS 40.0%
CACTAACTTTGGATCACTTC+TGG + Chr8:52990589-52990608 MsG0880045031.01.T01:CDS 40.0%
ATCTATTCCTCCCAACTCTC+AGG + Chr8:52990476-52990495 MsG0880045031.01.T01:CDS 45.0%
ATGTGAGATCTCCTGAGAGT+TGG - Chr8:52990490-52990509 None:intergenic 45.0%
TGTGAGATCTCCTGAGAGTT+GGG - Chr8:52990489-52990508 None:intergenic 45.0%
TTGTGAATTTGCGGATCCAG+CGG + Chr8:52990529-52990548 MsG0880045031.01.T01:CDS 45.0%
! AGTCCTTTAAAAGATCCCGC+TGG - Chr8:52990548-52990567 None:intergenic 45.0%
! GATCCAGCGGGATCTTTTAA+AGG + Chr8:52990542-52990561 MsG0880045031.01.T01:CDS 45.0%
TGTGAATTTGCGGATCCAGC+GGG + Chr8:52990530-52990549 MsG0880045031.01.T01:CDS 50.0%
GAGATCTCCTGAGAGTTGGG+AGG - Chr8:52990486-52990505 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 52990303 52990697 52990303 ID=MsG0880045031.01;Name=MsG0880045031.01
Chr8 mRNA 52990303 52990697 52990303 ID=MsG0880045031.01.T01;Parent=MsG0880045031.01;Name=MsG0880045031.01.T01;_AED=0.46;_eAED=0.64;_QI=0|0|0|1|0|0|2|0|92
Chr8 exon 52990303 52990343 52990303 ID=MsG0880045031.01.T01:exon:41615;Parent=MsG0880045031.01.T01
Chr8 exon 52990460 52990697 52990460 ID=MsG0880045031.01.T01:exon:41614;Parent=MsG0880045031.01.T01
Chr8 CDS 52990303 52990343 52990303 ID=MsG0880045031.01.T01:cds;Parent=MsG0880045031.01.T01
Chr8 CDS 52990460 52990697 52990460 ID=MsG0880045031.01.T01:cds;Parent=MsG0880045031.01.T01
Gene Sequence

>MsG0880045031.01.T01

ATGCTGAGAACAAAAAGAGGCATTGCTGAAGTGTACTCTCAATGGTTCACATGGCTGATCTATTCCTCCCAACTCTCAGGAGATCTCACATTGAATGCATATGAAGAGGATTGTGAATTTGCGGATCCAGCGGGATCTTTTAAAGGACTTCAGCGTTTCAAAAGGAACTGCACTAACTTTGGATCACTTCTGGAGAAGTCAACTATGAATCTTATGAAGTGGGAAGATTTTGAGGTGAAGATAACCTTGAACACTTTTCTACTGCTTTCTCAAATATAG

Protein sequence

>MsG0880045031.01.T01

MLRTKRGIAEVYSQWFTWLIYSSQLSGDLTLNAYEEDCEFADPAGSFKGLQRFKRNCTNFGSLLEKSTMNLMKWEDFEVKITLNTFLLLSQI*