AlfalfaGEDB | Msa0490140

M. sativa cultivar zhongmu-4 / Msa0490140

KEGG: -
GO: -
PFAM: PF12776.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0490140	XP_003589014.1	91.925	322	25	1	1	322	1	321	0.0	605

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0490140	sp\|Q9FFJ8\|LIMYB_ARATH	25.237	317	212	6	6	322	156	447	4.89e-23	102
Msa0490140	sp\|Q9FFJ8\|LIMYB_ARATH	36.036	111	70	1	20	130	2	111	2.01e-15	80.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0490140	G7IB81	91.925	322	25	1	1	322	1	321	0.0	605

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015390	Msa0490140	0.805100	1.571254e-49	-8.615850e-47
Msa0018940	Msa0490140	0.808507	2.994391e-50	-8.615850e-47
Msa0021210	Msa0490140	0.815765	7.817998e-52	-8.615850e-47
Msa0031520	Msa0490140	0.921050	5.877063e-88	-8.615850e-47
Msa0031710	Msa0490140	0.803229	3.851779e-49	-8.615850e-47
Msa0037880	Msa0490140	0.809294	2.031298e-50	-8.615850e-47
Msa0042920	Msa0490140	0.841389	4.896188e-58	-8.615850e-47
Msa0044260	Msa0490140	0.803046	4.201454e-49	-8.615850e-47
Msa0044330	Msa0490140	0.823193	1.580524e-53	-8.615850e-47
Msa0050200	Msa0490140	0.808237	3.419107e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0490140	MtrunA17_Chr2g0285481	62.963	324	114	4	2	321	1	322	3.95e-144	409
Msa0490140	MtrunA17_Chr1g0172741	62.346	324	116	4	2	321	1	322	9.53e-143	406
Msa0490140	MtrunA17_Chr8g0334551	57.012	328	134	2	1	321	1	328	3.60e-130	374
Msa0490140	MtrunA17_Chr2g0321391	67.213	244	77	2	79	321	3	244	1.86e-116	336
Msa0490140	MtrunA17_Chr5g0422701	65.174	201	67	2	122	321	14	212	1.52e-87	261
Msa0490140	MtrunA17_Chr2g0295811	41.629	221	111	6	119	321	10	230	1.26e-44	152
Msa0490140	MtrunA17_Chr5g0422691	57.143	105	42	2	2	103	1	105	6.39e-36	125
Msa0490140	MtrunA17_Chr2g0295611	62.500	96	35	1	100	194	1	96	3.04e-33	118
Msa0490140	MtrunA17_Chr8g0362511	28.803	309	185	9	21	310	67	359	8.51e-27	108
Msa0490140	MtrunA17_Chr5g0402821	28.481	316	196	9	22	315	16	323	9.65e-27	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0490140	AT5G05800.1	25.237	317	212	6	6	322	156	447	4.97e-24	102
Msa0490140	AT5G05800.1	36.036	111	70	1	20	130	2	111	2.05e-16	80.1
Msa0490140	AT5G05800.5	25.237	317	212	6	6	322	156	447	4.97e-24	102
Msa0490140	AT5G05800.5	36.036	111	70	1	20	130	2	111	2.05e-16	80.1
Msa0490140	AT5G05800.3	25.237	317	212	6	6	322	156	447	4.97e-24	102
Msa0490140	AT5G05800.3	36.036	111	70	1	20	130	2	111	2.05e-16	80.1
Msa0490140	AT5G05800.2	25.237	317	212	6	6	322	156	447	4.97e-24	102
Msa0490140	AT5G05800.2	36.036	111	70	1	20	130	2	111	2.05e-16	80.1
Msa0490140	AT5G05800.4	25.237	317	212	6	6	322	156	447	4.97e-24	102
Msa0490140	AT5G05800.4	36.036	111	70	1	20	130	2	111	2.05e-16	80.1

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Find 75 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGGTCAAAAGAG+TGG	0.632803	3_4:+11517518	Msa0490140:CDS
AAAAGGGTGGACAAATATTA+GGG	0.422862	3_4:+11516973	Msa0490140:CDS
AAACTTTGTGAAAGCATGTT+TGG	0.334075	3_4:+11516907	Msa0490140:CDS
AACCGGACTGAAGTATGAGA+AGG	0.626830	3_4:+11517012	Msa0490140:CDS
AACTTATTTGGGAGAGAAAC+TGG	0.287945	3_4:+11517088	Msa0490140:CDS
ACAACTGAATTTGGAAATAT+TGG	0.148866	3_4:+11517479	Msa0490140:CDS
ACACTTGGGCACCATCATGT+GGG	0.626839	3_4:+11517357	Msa0490140:CDS
AGAAGGATCTGGCGATAGCG+AGG	0.624952	3_4:+11517439	Msa0490140:CDS
AGAAGGCTCAACTAAAGAAT+AGG	0.479515	3_4:+11517029	Msa0490140:CDS
AGAGCATGGTATAACTTATT+TGG	0.134716	3_4:+11517076	Msa0490140:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATACCAAGAAAAATGTTG+TGG	+	chr3_4:11516820-11516839	Msa0490140:CDS	30.0%
GCAGAAAACAAAAGCTAATT+GGG	+	chr3_4:11516868-11516887	Msa0490140:CDS	30.0%
AAAAAGGGTGGACAAATATT+AGG	+	chr3_4:11516972-11516991	Msa0490140:CDS	30.0%
AAAAGGGTGGACAAATATTA+GGG	+	chr3_4:11516973-11516992	Msa0490140:CDS	30.0%
GTCCGATTTCATAATTTAAC+CGG	+	chr3_4:11516995-11517014	Msa0490140:CDS	30.0%
GTCCGGTTAAATTATGAAAT+CGG	-	chr3_4:11517000-11517019	None:intergenic	30.0%
AGAGCATGGTATAACTTATT+TGG	+	chr3_4:11517076-11517095	Msa0490140:CDS	30.0%
GAGCATGGTATAACTTATTT+GGG	+	chr3_4:11517077-11517096	Msa0490140:CDS	30.0%
TGTATTACATACCAGTTGTT+TGG	-	chr3_4:11517170-11517189	None:intergenic	30.0%
AACACGAGTATACATATCTA+AGG	-	chr3_4:11517213-11517232	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	11516815	11517880	11516815	ID=Msa0490140;Name=Msa0490140
chr3_4	mRNA	11516815	11517880	11516815	ID=Msa0490140-mRNA-1;Parent=Msa0490140;Name=Msa0490140-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|1\|0\|1\|1\|1\|2\|0\|326
chr3_4	exon	11516815	11517177	11516815	ID=Msa0490140-mRNA-1:exon:3558;Parent=Msa0490140-mRNA-1
chr3_4	exon	11517263	11517880	11517263	ID=Msa0490140-mRNA-1:exon:3559;Parent=Msa0490140-mRNA-1
chr3_4	CDS	11516815	11517177	11516815	ID=Msa0490140-mRNA-1:cds;Parent=Msa0490140-mRNA-1
chr3_4	CDS	11517263	11517880	11517263	ID=Msa0490140-mRNA-1:cds;Parent=Msa0490140-mRNA-1

Gene Sequence

>Msa0490140

ATGGTGAATACCAAGAAAAATGTTGTGGCTTCCTCCTCACCTAGTGATGGGGGGCAGAAAACAAAAGCTAATTGGGATTATAAGTCAACTGAAAACTTTGTGAAAGCATGTTTGGAGCAAGTCTCCAAGGGAGAACGTGTTGGTACGAGCTTTTCAAAAAAAGGGTGGACAAATATTAGGGTCCGATTTCATAATTTAACCGGACTGAAGTATGAGAAGGCTCAACTAAAGAATAGGTATGACAGTCTGAGAAAGGATTGGAGAGCATGGTATAACTTATTTGGGAGAGAAACTGGATTAGGGTGGGATCCTGTGAACAACACTGTTGTAGCACCTGATGAGTGGTGGGAGACCAAACAACTGGAAAACCCTCTTTATGAAAAATTTAGGAACAAGGGGCTTCCATTCGCCAATGAGTTAACCACACTTTTCAAGGATGTAGTGGCTAATGGAGAGTACACTTGGGCACCATCATGTGGGGTATTGCCATCTGTGTTTGAAAATGATGATAATGATGATGATGATGAGGCAGTGGCTGTAGAAGGATCTGGCGATAGCGAGGATGCAAGTGTTGGAGCAACAACTGAATTTGGAAATATTGGGTTGAATTCTTCACAAAAAACTGATGGTCAAAAGAGTGGTGAGAAGAGGAAAAGAGCTGTTAGAGCAGACAAGCCAAGTAAGAAAAAAGCAAGTGCATCATCAAAGATAGCTGAAGCTGTTAGTTTAATTGCAGAGACATGTCGTTCTCGCAATGATGCTGCGGGTAATGCATCTATTGGTGAAGTGATGGCTGAGCTTCACACCATGGATGAAGTCTCAAATGATCTTTATTTGCATGCTCGGTGTTGCAACTTGCTGATGTTTAAGCCTGCAAGGGAGATGTTTATTTCCCTACGAGGTTCAGAGGAGAAGAGGTTGAATTGGCTTAAGCATACAATTGATAACCCACTGCCATTCATGACTAACCTGTGTGACTAG

Protein sequence

>Msa0490140

MVNTKKNVVASSSPSDGGQKTKANWDYKSTENFVKACLEQVSKGERVGTSFSKKGWTNIRVRFHNLTGLKYEKAQLKNRYDSLRKDWRAWYNLFGRETGLGWDPVNNTVVAPDEWWETKQLENPLYEKFRNKGLPFANELTTLFKDVVANGEYTWAPSCGVLPSVFENDDNDDDDEAVAVEGSGDSEDASVGATTEFGNIGLNSSQKTDGQKSGEKRKRAVRADKPSKKKASASSKIAEAVSLIAETCRSRNDAAGNASIGEVMAELHTMDEVSNDLYLHARCCNLLMFKPAREMFISLRGSEEKRLNWLKHTIDNPLPFMTNLCD*