AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0247470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247470 MtrunA17_Chr2g0302391 94.872 429 19 2 1 426 1 429 0.0 799
Msa0247470 MtrunA17_Chr3g0099641 46.256 227 112 4 185 408 225 444 1.50e-58 198
Msa0247470 MtrunA17_Chr7g0270831 34.349 361 194 9 64 420 92 413 1.76e-51 179
Msa0247470 MtrunA17_Chr4g0075201 36.691 278 161 4 68 338 120 389 8.76e-49 173
Msa0247470 MtrunA17_Chr2g0322391 35.784 408 176 16 66 407 167 554 1.33e-48 174
Msa0247470 MtrunA17_Chr2g0288871 33.744 406 195 12 68 413 143 534 9.99e-48 171
Msa0247470 MtrunA17_Chr4g0025061 50.000 164 73 2 196 357 345 501 2.17e-41 155
Msa0247470 MtrunA17_Chr4g0025051 54.430 158 65 3 182 338 306 457 2.33e-40 152
Msa0247470 MtrunA17_Chr4g0051181 50.658 152 64 4 195 338 227 375 2.56e-37 142
Msa0247470 MtrunA17_Chr7g0216891 61.386 101 38 1 185 284 192 292 1.95e-36 140
Msa0247470 MtrunA17_Chr4g0024391 38.367 245 126 8 190 415 208 446 6.45e-34 132
Msa0247470 MtrunA17_Chr4g0060271 32.660 297 116 10 181 398 221 512 1.50e-32 129
Msa0247470 MtrunA17_Chr4g0001361 31.944 216 99 7 192 405 141 310 1.83e-25 105
Msa0247470 MtrunA17_Chr5g0432161 49.020 102 50 2 172 273 383 482 1.52e-23 103
Msa0247470 MtrunA17_Chr5g0432161 46.667 60 30 2 212 271 245 302 7.15e-11 64.3
Msa0247470 MtrunA17_Chr2g0333851 39.850 133 70 3 194 321 147 274 1.73e-22 97.4
Msa0247470 MtrunA17_Chr1g0150271 48.235 85 42 2 209 291 99 183 2.46e-22 95.5
Msa0247470 MtrunA17_Chr7g0270191 37.671 146 80 4 195 330 129 273 3.03e-22 96.3
Msa0247470 MtrunA17_Chr3g0136961 50.602 83 38 1 209 291 136 215 3.07e-22 96.3
Msa0247470 MtrunA17_Chr1g0150281 50.685 73 36 0 196 268 125 197 4.43e-22 95.5
Msa0247470 MtrunA17_Chr3g0079691 41.667 120 64 3 184 299 464 581 3.17e-21 96.3
Msa0247470 MtrunA17_Chr3g0079691 39.362 94 54 3 183 275 251 342 9.05e-14 73.6
Msa0247470 MtrunA17_Chr5g0445311 50.633 79 38 1 195 273 348 425 3.57e-21 95.9
Msa0247470 MtrunA17_Chr7g0263531 41.129 124 65 3 169 287 314 434 5.64e-21 94.7
Msa0247470 MtrunA17_Chr7g0263531 42.308 78 43 2 212 289 190 265 3.19e-12 68.2
Msa0247470 MtrunA17_Chr3g0106491 51.282 78 37 1 194 271 348 424 8.04e-21 94.7
Msa0247470 MtrunA17_Chr5g0419241 65.517 58 19 1 183 239 48 105 1.31e-20 86.7
Msa0247470 MtrunA17_Chr3g0101131 50.633 79 38 1 195 273 399 476 1.58e-20 94.0
Msa0247470 MtrunA17_Chr4g0004911 50.000 82 37 2 193 271 315 395 2.79e-20 92.8
Msa0247470 MtrunA17_Chr4g0004911 36.975 119 69 4 163 279 137 251 8.25e-13 70.1
Msa0247470 MtrunA17_Chr7g0218081 47.674 86 44 1 198 283 397 481 6.09e-20 92.0
Msa0247470 MtrunA17_Chr7g0218081 48.333 60 29 2 212 271 229 286 1.31e-11 66.2
Msa0247470 MtrunA17_Chr4g0042831 45.977 87 46 1 196 282 263 348 6.65e-20 90.9
Msa0247470 MtrunA17_Chr7g0249311 48.148 81 41 1 193 273 374 453 7.05e-20 92.4
Msa0247470 MtrunA17_Chr7g0249301 48.148 81 41 1 193 273 274 353 1.09e-19 90.1
Msa0247470 MtrunA17_Chr7g0249301 43.939 66 35 2 210 275 61 124 8.08e-11 63.5
Msa0247470 MtrunA17_Chr5g0418191 45.161 93 47 2 198 287 307 398 1.57e-19 90.1
Msa0247470 MtrunA17_Chr5g0418191 50.000 60 28 2 212 271 152 209 4.83e-12 67.4
Msa0247470 MtrunA17_Chr3g0129321 51.351 74 35 1 198 271 105 177 2.01e-19 86.3
Msa0247470 MtrunA17_Chr4g0068541 47.674 86 43 2 195 280 192 275 2.03e-19 89.7
Msa0247470 MtrunA17_Chr2g0294231 45.122 82 44 1 195 276 187 267 5.16e-19 88.2
Msa0247470 MtrunA17_Chr2g0289181 49.296 71 36 0 212 282 15 85 5.44e-19 85.5
Msa0247470 MtrunA17_Chr5g0447701 40.708 113 61 3 182 290 223 333 6.44e-19 88.2
Msa0247470 MtrunA17_Chr7g0224801 45.263 95 47 2 178 268 56 149 7.42e-19 83.6
Msa0247470 MtrunA17_Chr1g0194481 44.444 81 44 1 195 275 142 221 1.31e-18 86.3
Msa0247470 MtrunA17_Chr7g0248721 34.503 171 85 7 116 281 58 206 1.37e-18 84.3
Msa0247470 MtrunA17_Chr5g0443131 44.872 78 42 1 198 275 144 220 1.58e-18 85.9
Msa0247470 MtrunA17_Chr7g0243891 47.500 80 41 1 194 273 357 435 1.95e-18 87.4
Msa0247470 MtrunA17_Chr8g0383251 47.297 74 38 1 198 271 95 167 2.54e-18 82.4
Msa0247470 MtrunA17_Chr4g0051881 44.872 78 42 1 198 275 165 241 3.00e-18 85.5
Msa0247470 MtrunA17_Chr2g0284321 55.738 61 27 0 212 272 36 96 4.32e-18 82.8
Msa0247470 MtrunA17_Chr1g0150801 49.315 73 36 1 196 268 87 158 4.45e-18 81.3
Msa0247470 MtrunA17_Chr4g0053281 54.839 62 28 0 212 273 267 328 7.55e-18 84.3
Msa0247470 MtrunA17_Chr8g0379461 56.667 60 26 0 212 271 77 136 3.53e-17 80.9
Msa0247470 MtrunA17_Chr5g0401821 41.837 98 48 1 182 270 161 258 3.92e-17 81.3
Msa0247470 MtrunA17_Chr4g0074001 44.156 77 42 1 195 271 343 418 7.27e-17 82.8
Msa0247470 MtrunA17_Chr5g0418781 42.683 82 46 1 188 269 101 181 7.74e-17 80.5
Msa0247470 MtrunA17_Chr8g0386241 36.893 103 61 2 181 280 70 171 8.24e-17 78.2
Msa0247470 MtrunA17_Chr7g0270051 50.725 69 34 0 212 280 54 122 9.03e-17 80.1
Msa0247470 MtrunA17_Chr1g0184691 52.459 61 29 0 212 272 294 354 1.01e-16 81.3
Msa0247470 MtrunA17_Chr1g0188941 43.373 83 46 1 189 271 29 110 2.07e-16 75.1
Msa0247470 MtrunA17_Chr1g0147881 52.542 59 28 0 212 270 263 321 4.74e-16 79.0
Msa0247470 MtrunA17_Chr2g0319961 55.000 60 27 0 212 271 81 140 5.60e-16 77.4
Msa0247470 MtrunA17_Chr7g0228941 50.000 58 29 0 212 269 53 110 6.66e-16 73.9
Msa0247470 MtrunA17_Chr8g0335811 45.882 85 41 2 188 268 127 210 9.00e-16 76.3
Msa0247470 MtrunA17_Chr2g0293481 39.394 99 59 1 186 283 241 339 1.16e-15 79.0
Msa0247470 MtrunA17_Chr3g0129361 40.625 96 54 2 194 289 127 219 1.50e-15 75.5
Msa0247470 MtrunA17_Chr8g0348311 51.429 70 34 0 212 281 156 225 2.04e-15 77.0
Msa0247470 MtrunA17_Chr1g0202461 43.678 87 49 0 211 297 261 347 6.23e-15 76.3
Msa0247470 MtrunA17_Chr8g0387501 42.353 85 47 1 210 294 128 210 7.18e-15 74.7
Msa0247470 MtrunA17_Chr7g0248691 50.000 70 35 0 212 281 155 224 1.22e-14 74.7
Msa0247470 MtrunA17_Chr4g0071521 52.632 57 27 0 212 268 246 302 1.91e-14 73.9
Msa0247470 MtrunA17_Chr8g0383351 48.438 64 32 1 205 268 154 216 7.95e-14 70.9
Msa0247470 MtrunA17_Chr3g0139021 39.744 78 47 0 195 272 298 375 1.04e-13 72.4
Msa0247470 MtrunA17_Chr1g0189391 31.915 141 92 3 129 268 17 154 1.92e-13 71.2
Msa0247470 MtrunA17_Chr6g0485431 52.308 65 29 2 212 275 155 218 3.04e-13 70.9
Msa0247470 MtrunA17_Chr7g0238921 52.632 57 26 1 212 268 125 180 6.91e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247470 AT4G04450.2 37.942 311 143 7 70 338 20 322 1.25e-53 185
Msa0247470 AT4G04450.1 37.942 311 143 7 70 338 114 416 9.76e-53 184
Msa0247470 AT1G62300.1 35.392 421 172 17 74 419 158 553 9.77e-51 180
Msa0247470 AT4G01720.1 33.862 378 182 11 66 405 96 443 1.08e-44 162
Msa0247470 AT4G22070.4 36.533 323 151 9 66 341 108 423 1.97e-43 160
Msa0247470 AT4G22070.3 36.533 323 151 9 66 341 108 423 1.97e-43 160
Msa0247470 AT4G22070.2 36.533 323 151 9 66 341 108 423 1.97e-43 160
Msa0247470 AT4G22070.1 36.533 323 151 9 66 341 108 423 1.97e-43 160
Msa0247470 AT5G15130.1 29.712 451 195 13 68 412 35 469 2.72e-42 157
Msa0247470 AT5G15130.2 36.786 280 114 8 187 412 157 427 4.29e-42 155
Msa0247470 AT1G68150.1 64.815 108 37 1 189 296 213 319 5.52e-41 149
Msa0247470 AT1G18860.1 32.951 349 152 9 68 347 41 376 1.55e-36 140
Msa0247470 AT1G69810.1 44.937 158 74 3 185 340 177 323 1.23e-32 127
Msa0247470 AT1G69810.2 44.172 163 78 3 180 340 114 265 1.31e-32 126
Msa0247470 AT5G07100.2 39.130 138 73 4 146 280 81 210 3.68e-25 102
Msa0247470 AT5G07100.4 39.130 138 73 4 146 280 81 210 3.68e-25 102
Msa0247470 AT5G07100.5 39.130 138 73 4 146 280 81 210 3.68e-25 102
Msa0247470 AT5G07100.3 39.130 138 73 4 146 280 81 210 3.68e-25 102
Msa0247470 AT5G07100.1 38.571 140 75 4 144 280 172 303 6.51e-25 104
Msa0247470 AT1G80840.1 31.658 199 106 6 75 271 36 206 4.13e-22 96.3
Msa0247470 AT4G31800.2 56.944 72 30 1 205 275 104 175 6.33e-22 94.7
Msa0247470 AT4G31800.1 56.000 75 32 1 205 278 169 243 7.19e-22 95.9
Msa0247470 AT2G38470.1 42.857 119 64 3 156 273 308 423 8.24e-22 98.2
Msa0247470 AT2G38470.1 48.333 60 29 2 212 271 185 242 5.48e-11 64.7
Msa0247470 AT2G47260.1 48.810 84 42 1 195 278 158 240 1.37e-21 95.5
Msa0247470 AT1G29860.1 48.148 81 41 1 195 275 120 199 2.01e-21 94.0
Msa0247470 AT5G41570.1 48.352 91 42 2 182 268 65 154 2.62e-21 91.3
Msa0247470 AT2G30250.1 47.826 92 45 2 186 275 301 391 3.32e-21 95.1
Msa0247470 AT2G04880.2 49.351 77 38 1 197 273 269 344 4.83e-21 95.5
Msa0247470 AT2G25000.2 55.556 72 31 1 204 274 95 166 5.63e-21 91.7
Msa0247470 AT2G25000.4 55.556 72 31 1 204 274 95 166 5.63e-21 91.7
Msa0247470 AT2G04880.1 49.351 77 38 1 197 273 293 368 6.40e-21 95.1
Msa0247470 AT2G25000.3 55.556 72 31 1 204 274 117 188 8.96e-21 91.7
Msa0247470 AT4G18170.1 46.875 96 48 3 178 271 137 231 1.12e-20 92.8
Msa0247470 AT2G25000.1 55.556 72 31 1 204 274 138 209 1.18e-20 91.7
Msa0247470 AT1G64000.1 51.190 84 37 2 198 281 101 180 3.14e-20 88.6
Msa0247470 AT5G56270.1 48.864 88 44 1 186 273 462 548 4.23e-20 93.2
Msa0247470 AT4G26640.5 48.718 78 39 1 194 271 188 264 4.63e-20 91.7
Msa0247470 AT4G26640.6 48.718 78 39 1 194 271 188 264 4.63e-20 91.7
Msa0247470 AT4G26640.4 48.718 78 39 1 194 271 188 264 4.63e-20 91.7
Msa0247470 AT4G26640.3 48.718 78 39 1 194 271 188 264 4.63e-20 91.7
Msa0247470 AT4G26640.7 48.718 78 39 1 194 271 324 400 7.86e-20 92.0
Msa0247470 AT4G26640.1 48.718 78 39 1 194 271 292 368 8.84e-20 91.7
Msa0247470 AT4G26640.2 49.351 77 38 1 195 271 365 440 9.89e-20 92.0
Msa0247470 AT5G26170.1 52.778 72 33 1 197 268 99 169 1.51e-19 85.9
Msa0247470 AT1G13960.2 50.000 74 36 1 198 271 243 315 1.79e-19 89.7
Msa0247470 AT1G13960.2 50.000 60 28 2 212 271 77 134 1.31e-11 66.2
Msa0247470 AT1G13960.1 50.000 74 36 1 198 271 396 468 2.46e-19 90.5
Msa0247470 AT1G13960.1 50.000 60 28 2 212 271 230 287 9.32e-12 67.4
Msa0247470 AT3G62340.1 44.037 109 55 2 198 301 105 212 3.75e-19 87.4
Msa0247470 AT5G64810.1 48.276 87 41 2 183 269 85 167 4.65e-19 85.1
Msa0247470 AT2G03340.1 48.684 76 38 1 198 273 402 476 4.86e-19 89.7
Msa0247470 AT2G03340.1 51.667 60 27 2 212 271 251 308 9.23e-13 70.5
Msa0247470 AT2G46130.1 44.330 97 50 2 185 281 4 96 5.10e-19 82.4
Msa0247470 AT5G46350.1 41.667 96 54 2 198 293 170 263 5.14e-19 87.8
Msa0247470 AT5G13080.1 48.193 83 39 2 198 280 54 132 5.30e-19 83.6
Msa0247470 AT5G49520.1 45.161 93 49 2 195 286 205 296 5.36e-19 89.0
Msa0247470 AT3G01970.1 50.000 74 36 1 197 270 51 123 1.36e-18 82.4
Msa0247470 AT3G01080.1 48.649 74 37 1 198 271 293 365 1.54e-18 87.8
Msa0247470 AT3G01080.2 48.649 74 37 1 198 271 331 403 1.77e-18 87.8
Msa0247470 AT1G69310.4 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
Msa0247470 AT1G69310.2 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
Msa0247470 AT1G69310.1 45.238 84 45 1 186 269 122 204 2.32e-18 85.5
Msa0247470 AT4G26440.1 34.459 148 83 5 161 295 311 457 5.46e-18 86.7
Msa0247470 AT4G30935.1 38.136 118 67 3 182 295 303 418 7.38e-18 85.9
Msa0247470 AT2G37260.3 39.695 131 59 6 157 274 211 334 1.90e-17 83.6
Msa0247470 AT2G37260.3 44.156 77 39 3 211 287 85 157 2.90e-13 71.2
Msa0247470 AT2G37260.2 39.695 131 59 6 157 274 209 332 1.98e-17 83.6
Msa0247470 AT2G37260.2 44.156 77 39 3 211 287 83 155 3.11e-13 70.9
Msa0247470 AT2G23320.1 57.627 59 25 0 212 270 241 299 2.61e-17 82.8
Msa0247470 AT2G37260.1 41.221 131 57 7 157 274 291 414 3.38e-17 83.6
Msa0247470 AT2G37260.1 44.156 77 39 3 211 287 165 237 4.06e-13 71.2
Msa0247470 AT2G30590.1 54.688 64 28 1 212 274 314 377 7.24e-17 82.4
Msa0247470 AT5G28650.1 57.895 57 24 0 212 268 263 319 7.84e-17 81.6
Msa0247470 AT3G04670.1 57.895 57 24 0 212 268 263 319 1.11e-16 81.3
Msa0247470 AT4G24240.1 55.932 59 26 0 212 270 282 340 2.28e-16 80.5
Msa0247470 AT1G55600.1 42.697 89 46 2 189 273 281 368 3.26e-16 80.9
Msa0247470 AT1G29280.1 45.882 85 38 1 202 286 65 141 3.44e-16 78.6
Msa0247470 AT4G31550.2 44.872 78 40 1 212 289 246 320 4.40e-16 79.3
Msa0247470 AT4G31550.1 44.872 78 40 1 212 289 247 321 4.49e-16 79.3
Msa0247470 AT2G44745.1 43.956 91 46 2 182 268 112 201 4.67e-16 77.4
Msa0247470 AT2G24570.1 53.448 58 27 0 212 269 244 301 1.10e-15 78.2
Msa0247470 AT1G30650.1 43.529 85 47 1 188 271 193 277 1.48e-15 78.6
Msa0247470 AT2G34830.1 43.529 85 47 1 188 271 191 275 1.62e-15 78.6
Msa0247470 AT2G34830.2 43.529 85 47 1 188 271 200 284 2.10e-15 78.2
Msa0247470 AT4G01250.1 48.649 74 38 0 208 281 125 198 2.28e-15 76.6
Msa0247470 AT4G23550.1 43.373 83 47 0 209 291 138 220 3.05e-15 76.6
Msa0247470 AT3G58710.1 55.000 60 27 0 212 271 72 131 4.11e-15 75.5
Msa0247470 AT3G58710.2 55.000 60 27 0 212 271 71 130 4.50e-15 75.5
Msa0247470 AT5G52830.1 50.000 70 35 0 212 281 166 235 1.46e-14 75.1
Msa0247470 AT2G46130.2 42.105 95 47 3 187 281 6 92 3.10e-14 68.9
Msa0247470 AT4G39410.1 40.000 85 50 1 184 268 196 279 6.20e-14 72.8
Msa0247470 AT1G69310.3 41.667 84 44 2 186 269 122 200 1.56e-13 71.2
Msa0247470 AT5G45050.1 36.842 114 69 1 212 322 1181 1294 1.73e-13 73.2
Msa0247470 AT5G45050.2 36.842 114 69 1 212 322 1153 1266 1.74e-13 73.2
Msa0247470 AT5G45050.4 36.842 114 69 1 212 322 1042 1155 1.95e-13 73.2
Msa0247470 AT5G43290.1 47.541 61 31 1 212 272 115 174 3.31e-12 67.0
Msa0247470 AT4G31800.3 62.222 45 16 1 205 248 169 213 4.21e-12 66.2
Msa0247470 AT2G21900.1 46.667 60 31 1 212 271 110 168 5.12e-12 65.1
Msa0247470 AT2G46400.1 36.000 100 62 1 190 287 83 182 3.66e-11 64.3
Msa0247470 AT2G40740.1 43.902 82 35 3 212 282 174 255 4.13e-11 63.9
Msa0247470 AT2G40740.3 43.902 82 35 3 212 282 183 264 5.30e-11 63.9

Find 107 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATTGTTTATCATATT+TGG 0.055350 2_2:+29658534 None:intergenic
CACTACTCTTACTAGGTAAT+TGG 0.179010 2_2:+29658391 None:intergenic
ATTAATCACTTACTTGGATT+TGG 0.221328 2_2:+29661662 None:intergenic
GGAGCAATGATGATTGTTTA+TGG 0.223525 2_2:+29658598 None:intergenic
AACACAAAGGCTACATAAAA+TGG 0.242040 2_2:-29662249 Msa0247470:exon
TTAATCACTTACTTGGATTT+GGG 0.256367 2_2:+29661663 None:intergenic
ATGCATAAACTATATATATA+AGG 0.261334 2_2:+29662311 None:intergenic
GAAAACTTACAGTATTCTTA+TGG 0.285405 2_2:+29662123 None:intergenic
TCTTCAGAATCGTTTACTTT+TGG 0.317553 2_2:-29661345 Msa0247470:CDS
AGCCTACTATCGTTGCAGTA+TGG 0.326812 2_2:-29660393 Msa0247470:CDS
GGGTGTCAATGGAGAAAATA+TGG 0.328698 2_2:-29660449 Msa0247470:CDS
GGGGATTTCCTTGAATTTGA+TGG 0.332291 2_2:+29661683 None:intergenic
AAAATCTGATCGTGCCCTTA+TGG 0.343745 2_2:+29660599 None:intergenic
TCTTACTAGGTAATTGGTTT+CGG 0.350120 2_2:+29658397 None:intergenic
GTAGCTATAGCATTTGAGTT+TGG 0.354243 2_2:+29658699 None:intergenic
TCTTTATTGTTATTATCATA+TGG 0.359665 2_2:+29662179 None:intergenic
TAGTTTACCTGCTTACGAAC+TGG 0.359755 2_2:+29660359 None:intergenic
GAAGTTGTGTTGTGTTATTA+AGG 0.369494 2_2:+29661707 None:intergenic
GATGATGTTAGGTTTGCTAT+TGG 0.371407 2_2:+29658819 None:intergenic
TGCATAAACTATATATATAA+GGG 0.377095 2_2:+29662312 None:intergenic
AATTAACCACTACTCTTACT+AGG 0.379801 2_2:+29658384 None:intergenic
CTTAAGAGGTATGCTAAATC+TGG 0.384344 2_2:-29661387 Msa0247470:CDS
TTTGAGTTTGGTGGAGATAA+TGG 0.386272 2_2:+29658711 None:intergenic
TCAACAAATCTACAACAATA+TGG 0.389536 2_2:-29658768 Msa0247470:CDS
ATGATTATGGCTCCCTTCAT+AGG 0.402020 2_2:+29658863 None:intergenic
CAATGAGAGAAAACTTGCTT+TGG 0.409723 2_2:-29658520 Msa0247470:CDS
TGTTGAGTGCTGATGATGTT+AGG 0.413129 2_2:+29658808 None:intergenic
CAGCCAGCAGATTCAGTTGA+TGG 0.415892 2_2:+29662340 None:intergenic
AGTTCAAACAATGAAATTGC+TGG 0.417191 2_2:-29662219 Msa0247470:CDS
CGATAGTAGGCTCGTGGACA+AGG 0.430122 2_2:+29660404 None:intergenic
GTAAAATCAAGTGTAATTGT+AGG 0.435039 2_2:+29658654 None:intergenic
GGAGAGCTAGAGTGTCCATA+AGG 0.442520 2_2:-29660614 Msa0247470:CDS
GGGATTTCCTTGAATTTGAT+GGG 0.451310 2_2:+29661684 None:intergenic
AAGGAGAATGAAAACTTAAG+AGG 0.454898 2_2:-29661401 Msa0247470:CDS
AAATAATGGATTTGCCATAG+TGG 0.457421 2_2:-29660739 Msa0247470:CDS
CTGGTGGAAGTTGATGATTA+TGG 0.462621 2_2:+29658850 None:intergenic
TGAAATAAATAGCAAAATAG+AGG 0.463782 2_2:-29660661 Msa0247470:CDS
CAACTTCCCCATCAAATTCA+AGG 0.467550 2_2:-29661691 Msa0247470:CDS
GCCTACTATCGTTGCAGTAT+GGG 0.470435 2_2:-29660392 Msa0247470:CDS
GGAAACACCTCAAATGATTG+AGG 0.470497 2_2:+29658555 None:intergenic
TGCACCCAACCTCAGATGAT+TGG 0.470879 2_2:+29660637 None:intergenic
GCTATAGCATTTGAGTTTGG+TGG 0.472660 2_2:+29658702 None:intergenic
GGGTAACCTTGGAATGGAAA+AGG 0.483048 2_2:+29658576 None:intergenic
TTGAATTGTAAATAGTTACT+TGG 0.483082 2_2:+29658624 None:intergenic
CCCCATACTGCAACGATAGT+AGG 0.503349 2_2:+29660391 None:intergenic
GAAACACCTCAAATGATTGA+GGG 0.504461 2_2:+29658556 None:intergenic
GAAGCTTGATGAAGCAAAGA+AGG 0.508309 2_2:-29661420 Msa0247470:CDS
AGGACCAATCATCTGAGGTT+GGG 0.511884 2_2:-29660641 Msa0247470:CDS
AGTTTACCTGCTTACGAACT+GGG 0.512250 2_2:+29660360 None:intergenic
CAAATGATTGAGGGTAACCT+TGG 0.513680 2_2:+29658565 None:intergenic
GAGAGCTAGAGTGTCCATAA+GGG 0.513998 2_2:-29660613 Msa0247470:CDS
TTCATGCCCAGTTCGTAAGC+AGG 0.514284 2_2:-29660366 Msa0247470:intron
CTTAATAAAAGAGTAGATCC+TGG 0.516100 2_2:+29661736 None:intergenic
GCCATGTCCTCATTCACAAA+TGG 0.516277 2_2:-29658438 Msa0247470:CDS
ATGATCTCCATTTGTGAATG+AGG 0.518687 2_2:+29658431 None:intergenic
GTGTTCATCACAACCTATGA+AGG 0.519912 2_2:-29658876 Msa0247470:CDS
AGTATTCTTATGGAAGGAAG+AGG 0.520852 2_2:+29662133 None:intergenic
TAAAATCAAGTGTAATTGTA+GGG 0.521053 2_2:+29658655 None:intergenic
AAATAATCACAACTTGCTGC+TGG 0.523498 2_2:-29661288 Msa0247470:intron
GAGGACCAATCATCTGAGGT+TGG 0.528239 2_2:-29660642 Msa0247470:CDS
AGATGGTCGATGGGTGTCAA+TGG 0.529990 2_2:-29660460 Msa0247470:CDS
TCGTGGACAAGGGTTGCCCT+TGG 0.531338 2_2:+29660415 None:intergenic
ATTGTAGGGCATGTTGGAGA+TGG 0.532259 2_2:+29658669 None:intergenic
AGTGTAATTGTAGGGCATGT+TGG 0.533130 2_2:+29658663 None:intergenic
AATTGTAAATAGTTACTTGG+AGG 0.533159 2_2:+29658627 None:intergenic
GTTCCATCAACTGAATCTGC+TGG 0.533550 2_2:-29662343 Msa0247470:five_prime_UTR
AATCATCTGAGGTTGGGTGC+AGG 0.533609 2_2:-29660635 Msa0247470:CDS
TTGCTACAATCTGAAGGTGA+AGG 0.539687 2_2:+29660762 None:intergenic
GAGATAATGGTGAAGAGAAG+AGG 0.540861 2_2:+29658724 None:intergenic
TTGCTACACCGTTGTACAGT+GGG 0.547099 2_2:+29658335 None:intergenic
GCATGTGAGTACATTAATGA+AGG 0.552654 2_2:-29660684 Msa0247470:CDS
GATTGAGGGTAACCTTGGAA+TGG 0.552885 2_2:+29658570 None:intergenic
AGGTTACCCTCAATCATTTG+AGG 0.553978 2_2:-29658562 Msa0247470:CDS
GGTAACCTTGGAATGGAAAA+GGG 0.559036 2_2:+29658577 None:intergenic
TGTAATGTTTAGATGGTCGA+TGG 0.565059 2_2:-29660470 Msa0247470:intron
TGTAGATTTGTTGATGAGGT+AGG 0.565582 2_2:+29658777 None:intergenic
GATTTGTTGATGAGGTAGGA+AGG 0.569280 2_2:+29658781 None:intergenic
AACTGACGAGTAGGTAACAC+TGG 0.572998 2_2:+29660795 None:intergenic
ACTTACAGTATTCTTATGGA+AGG 0.574288 2_2:+29662127 None:intergenic
ATTGCAATTAACAGGTGCAA+AGG 0.574633 2_2:-29658917 Msa0247470:intron
TAATCACTTACTTGGATTTG+GGG 0.580090 2_2:+29661664 None:intergenic
AATAATGCTTCCTTGAGAGA+AGG 0.583998 2_2:+29658465 None:intergenic
CCTACTATCGTTGCAGTATG+GGG 0.587972 2_2:-29660391 Msa0247470:CDS
TTATTGTTATTATCATATGG+TGG 0.589100 2_2:+29662182 None:intergenic
ACACCGTTGTACAGTGGGAG+AGG 0.598219 2_2:+29658340 None:intergenic
TTTGCTACACCGTTGTACAG+TGG 0.607038 2_2:+29658334 None:intergenic
GTAATGTTTAGATGGTCGAT+GGG 0.613347 2_2:-29660469 Msa0247470:intron
GAAGGGAAATACACAAGATG+CGG 0.615352 2_2:-29660823 Msa0247470:CDS
TCATCAAAGAACTGACGAGT+AGG 0.619422 2_2:+29660786 None:intergenic
TATGGACAAAAGACAGCCAA+GGG 0.623915 2_2:-29660431 Msa0247470:CDS
ATATGGACAAAAGACAGCCA+AGG 0.629423 2_2:-29660432 Msa0247470:CDS
CTGCAACGATAGTAGGCTCG+TGG 0.637106 2_2:+29660398 None:intergenic
TCCATTTGTGAATGAGGACA+TGG 0.643304 2_2:+29658437 None:intergenic
GATAGTAGGCTCGTGGACAA+GGG 0.644110 2_2:+29660405 None:intergenic
GCATAAACTATATATATAAG+GGG 0.644507 2_2:+29662313 None:intergenic
GGAAAACAATGAAAACAACA+TGG 0.645971 2_2:-29660718 Msa0247470:CDS
TGTTCATCACAACCTATGAA+GGG 0.646804 2_2:-29658875 Msa0247470:CDS
GGATTTCCTTGAATTTGATG+GGG 0.651214 2_2:+29661685 None:intergenic
AATAGAGGACCAATCATCTG+AGG 0.655874 2_2:-29660646 Msa0247470:CDS
GATCCTCTCCCACTGTACAA+CGG 0.660347 2_2:-29658343 Msa0247470:three_prime_UTR
CAATTACCTAGTAAGAGTAG+TGG 0.667381 2_2:-29658390 Msa0247470:CDS
ATAGACTTAGCAAAGACTAG+TGG 0.669454 2_2:-29662375 Msa0247470:five_prime_UTR
GAAAACAATGAAAACAACAT+GGG 0.672904 2_2:-29660717 Msa0247470:CDS
TTGTTGTAGATTTGTTGATG+AGG 0.675654 2_2:+29658773 None:intergenic
AATTGCTGGAAGTTGATGAA+AGG 0.676322 2_2:-29662205 Msa0247470:CDS
GCTGCTCTATAGTTCATGTG+AGG 0.677528 2_2:+29662276 None:intergenic
TAGAGCAGCAATCAACACAA+AGG 0.685681 2_2:-29662262 Msa0247470:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATCAAATATCAAATA+TGG + chr2_2:29658655-29658674 None:intergenic 10.0%
!!! AATAACATTTTTTAGAATTT+AGG + chr2_2:29660546-29660565 None:intergenic 10.0%
!!! TTTTATATGTATAATAGAAA+TGG - chr2_2:29660728-29660747 Msa0247470:CDS 10.0%
!!! ATCTATTTTCAAATATTATA+AGG - chr2_2:29661257-29661276 Msa0247470:intron 10.0%
!!! TCTATTTTCAAATATTATAA+GGG - chr2_2:29661258-29661277 Msa0247470:intron 10.0%
!! TGCATAAACTATATATATAA+GGG + chr2_2:29658409-29658428 None:intergenic 15.0%
!! ATGCATAAACTATATATATA+AGG + chr2_2:29658410-29658429 None:intergenic 15.0%
!!! TCTTTATTGTTATTATCATA+TGG + chr2_2:29658542-29658561 None:intergenic 15.0%
!!! GATAAATTAAACTCTAATTT+TGG - chr2_2:29659763-29659782 Msa0247470:intron 15.0%
!! TTTCTTCATAATTAATTAGA+AGG - chr2_2:29659877-29659896 Msa0247470:intron 15.0%
!! TTCTTCATAATTAATTAGAA+GGG - chr2_2:29659878-29659897 Msa0247470:intron 15.0%
!! CAAAATATAAGAATTTCAAT+TGG - chr2_2:29660498-29660517 Msa0247470:intron 15.0%
!!! ATTTTTGTTTTTTATGTTTG+TGG - chr2_2:29660580-29660599 Msa0247470:intron 15.0%
!!! AATTTTAATGCATTGAAAAA+GGG - chr2_2:29660851-29660870 Msa0247470:intron 15.0%
!!! AAAAGTTTTTGTAATGTAAT+TGG + chr2_2:29660910-29660929 None:intergenic 15.0%
!! TTAAATTTCTATCAAAGATA+TGG - chr2_2:29660963-29660982 Msa0247470:intron 15.0%
!!! ATTTTCAAATATTTCGAAAA+TGG - chr2_2:29661229-29661248 Msa0247470:intron 15.0%
!!! TTTTCAAATATTTCGAAAAT+GGG - chr2_2:29661230-29661249 Msa0247470:intron 15.0%
!! GCATAAACTATATATATAAG+GGG + chr2_2:29658408-29658427 None:intergenic 20.0%
!!! TTATTGTTATTATCATATGG+TGG + chr2_2:29658539-29658558 None:intergenic 20.0%
!!! TAATAACTTGACATTTTGAT+GGG - chr2_2:29658947-29658966 Msa0247470:intron 20.0%
!! AATAAGATTAATCACTTACT+TGG + chr2_2:29659065-29659084 None:intergenic 20.0%
!! ATTTAATGATCTCATGTATT+TGG - chr2_2:29659534-29659553 Msa0247470:intron 20.0%
!! TTTATCTATTTGTAAGTAGA+CGG + chr2_2:29659613-29659632 None:intergenic 20.0%
!!! TGTTGTTTATGTAATTAACA+TGG - chr2_2:29659689-29659708 Msa0247470:intron 20.0%
!!! TTAAACTCTAATTTTGGATT+TGG - chr2_2:29659769-29659788 Msa0247470:intron 20.0%
!!! TTACAAGTTGAAAATATCAT+AGG + chr2_2:29659822-29659841 None:intergenic 20.0%
!! TATGAAGAAAAAATGTACAA+AGG + chr2_2:29659867-29659886 None:intergenic 20.0%
!! TGAAATAAATAGCAAAATAG+AGG - chr2_2:29660057-29660076 Msa0247470:intron 20.0%
!! ATCAATTTGTAATGTTTAGA+TGG - chr2_2:29660241-29660260 Msa0247470:intron 20.0%
!! TAGGTTATATCATATCAAAA+TGG + chr2_2:29660407-29660426 None:intergenic 20.0%
!!! CATTTTTTAGAATTTAGGTT+GGG + chr2_2:29660541-29660560 None:intergenic 20.0%
!!! ACATTTTTTAGAATTTAGGT+TGG + chr2_2:29660542-29660561 None:intergenic 20.0%
!!! CAATTTTAATGCATTGAAAA+AGG - chr2_2:29660850-29660869 Msa0247470:intron 20.0%
!!! ATTTTAATGCATTGAAAAAG+GGG - chr2_2:29660852-29660871 Msa0247470:intron 20.0%
!!! TTCAATTGATAATATATGCA+AGG - chr2_2:29661025-29661044 Msa0247470:intron 20.0%
!! TAACCGAACAATAAATAAAA+TGG - chr2_2:29661113-29661132 Msa0247470:intron 20.0%
!!! CTTCCATTTTATTTATTGTT+CGG + chr2_2:29661119-29661138 None:intergenic 20.0%
!! TTTCAAATATTTCGAAAATG+GGG - chr2_2:29661231-29661250 Msa0247470:intron 20.0%
!!! AATTCTCATTTCATTCTTTT+AGG + chr2_2:29661459-29661478 None:intergenic 20.0%
!! AAAATTCAAGAACTAACTAT+TGG + chr2_2:29661518-29661537 None:intergenic 20.0%
!!! ATAATGATGAGACTATTTTA+AGG - chr2_2:29661685-29661704 Msa0247470:CDS 20.0%
!!! TGTGTAATTCTGTTTTAAAA+TGG - chr2_2:29661721-29661740 Msa0247470:CDS 20.0%
!! TTAATTACATTGCAATTAAC+AGG - chr2_2:29661793-29661812 Msa0247470:intron 20.0%
!! TAAAATCAAGTGTAATTGTA+GGG + chr2_2:29662066-29662085 None:intergenic 20.0%
!! TTGAATTGTAAATAGTTACT+TGG + chr2_2:29662097-29662116 None:intergenic 20.0%
! GAAAACTTACAGTATTCTTA+TGG + chr2_2:29658598-29658617 None:intergenic 25.0%
!! AAGAGTCATATACTTTGTTT+AGG - chr2_2:29658745-29658764 Msa0247470:CDS 25.0%
! TTAGCTTATGATGAAAATCA+TGG - chr2_2:29658853-29658872 Msa0247470:CDS 25.0%
! TAGCTTATGATGAAAATCAT+GGG - chr2_2:29658854-29658873 Msa0247470:CDS 25.0%
! AAATGTCAAGTTATTAGGTT+AGG + chr2_2:29658943-29658962 None:intergenic 25.0%
! CATCAAAATGTCAAGTTATT+AGG + chr2_2:29658948-29658967 None:intergenic 25.0%
!! CTAATAACTTGACATTTTGA+TGG - chr2_2:29658946-29658965 Msa0247470:intron 25.0%
! TTAATCACTTACTTGGATTT+GGG + chr2_2:29659058-29659077 None:intergenic 25.0%
! ATTAATCACTTACTTGGATT+TGG + chr2_2:29659059-29659078 None:intergenic 25.0%
!!! TTTGATAATTGCTCTAAGAT+TGG - chr2_2:29659263-29659282 Msa0247470:intron 25.0%
! AAATGAGTGTGAATCAAATA+AGG + chr2_2:29659487-29659506 None:intergenic 25.0%
! TTATCTATTTGTAAGTAGAC+GGG + chr2_2:29659612-29659631 None:intergenic 25.0%
!!! TTATTTTTGCTACAATCTGA+AGG + chr2_2:29659965-29659984 None:intergenic 25.0%
! TCAGATTGTAGCAAAAATAA+TGG - chr2_2:29659965-29659984 Msa0247470:intron 25.0%
! GAAAACAATGAAAACAACAT+GGG - chr2_2:29660001-29660020 Msa0247470:intron 25.0%
!! TCGAACTTATGATAGTTTTT+AGG + chr2_2:29660426-29660445 None:intergenic 25.0%
! ATGTAATGAATGTTTGTGAA+AGG - chr2_2:29661143-29661162 Msa0247470:intron 25.0%
! TTCCTTATAATGCTAATTAG+TGG + chr2_2:29661380-29661399 None:intergenic 25.0%
! ATTATAAGGAAGATGATGAT+GGG - chr2_2:29661389-29661408 Msa0247470:CDS 25.0%
! AAAGTCATGAATACATCATA+AGG - chr2_2:29661661-29661680 Msa0247470:intron 25.0%
! TCAACAAATCTACAACAATA+TGG - chr2_2:29661950-29661969 Msa0247470:intron 25.0%
! GTAAAATCAAGTGTAATTGT+AGG + chr2_2:29662067-29662086 None:intergenic 25.0%
! AATTGTAAATAGTTACTTGG+AGG + chr2_2:29662094-29662113 None:intergenic 25.0%
! CTCTCATTGTTTATCATATT+TGG + chr2_2:29662187-29662206 None:intergenic 25.0%
! GTGGTTAATTGTAAAAAGTT+TGG - chr2_2:29662347-29662366 Msa0247470:five_prime_UTR 25.0%
! AATTGTAAAAAGTTTGGACT+CGG - chr2_2:29662353-29662372 Msa0247470:five_prime_UTR 25.0%
AACACAAAGGCTACATAAAA+TGG - chr2_2:29658469-29658488 Msa0247470:CDS 30.0%
AGTTCAAACAATGAAATTGC+TGG - chr2_2:29658499-29658518 Msa0247470:CDS 30.0%
ACTTACAGTATTCTTATGGA+AGG + chr2_2:29658594-29658613 None:intergenic 30.0%
TAATCCAGTATACACAAACA+TGG + chr2_2:29658628-29658647 None:intergenic 30.0%
CTTAATAAAAGAGTAGATCC+TGG + chr2_2:29658985-29659004 None:intergenic 30.0%
! GAAGTTGTGTTGTGTTATTA+AGG + chr2_2:29659014-29659033 None:intergenic 30.0%
TAATCACTTACTTGGATTTG+GGG + chr2_2:29659057-29659076 None:intergenic 30.0%
AAGGAGAATGAAAACTTAAG+AGG - chr2_2:29659317-29659336 Msa0247470:intron 30.0%
! TCTTCAGAATCGTTTACTTT+TGG - chr2_2:29659373-29659392 Msa0247470:intron 30.0%
!! AGCAAGTTGTGATTATTTTG+AGG + chr2_2:29659428-29659447 None:intergenic 30.0%
!!! AATTTTGGATTTGGTGAATG+TGG - chr2_2:29659778-29659797 Msa0247470:intron 30.0%
!! AAATAATGGATTTGCCATAG+TGG - chr2_2:29659979-29659998 Msa0247470:intron 30.0%
! GTTTTCATTGTTTTCCACTA+TGG + chr2_2:29659996-29660015 None:intergenic 30.0%
GGAAAACAATGAAAACAACA+TGG - chr2_2:29660000-29660019 Msa0247470:intron 30.0%
! GATCAGATTTTGCATTTGTA+AGG - chr2_2:29660131-29660150 Msa0247470:intron 30.0%
! TCGATTTTATAGAAGTGAGT+TGG - chr2_2:29660467-29660486 Msa0247470:intron 30.0%
! CGGACAAACTATTAATTTGA+GGG - chr2_2:29660640-29660659 Msa0247470:CDS 30.0%
! GGACAAACTATTAATTTGAG+GGG - chr2_2:29660641-29660660 Msa0247470:CDS 30.0%
!! GCAAATATGTTACTTTGTTC+TGG - chr2_2:29660784-29660803 Msa0247470:CDS 30.0%
TTGTAGTGCATCATCAATTA+AGG + chr2_2:29660817-29660836 None:intergenic 30.0%
! GACTATTAGTTCTTACTAGA+TGG - chr2_2:29660988-29661007 Msa0247470:intron 30.0%
! TATTAGTTCTTACTAGATGG+AGG - chr2_2:29660991-29661010 Msa0247470:intron 30.0%
! TTGATAATATATGCAAGGTC+TGG - chr2_2:29661030-29661049 Msa0247470:intron 30.0%
TGATAATATATGCAAGGTCT+GGG - chr2_2:29661031-29661050 Msa0247470:intron 30.0%
ATGAATGTTTGTGAAAGGAA+AGG - chr2_2:29661148-29661167 Msa0247470:intron 30.0%
!! AGGGTATTGTTTCTCATATT+AGG - chr2_2:29661277-29661296 Msa0247470:intron 30.0%
! GGGTATTGTTTCTCATATTA+GGG - chr2_2:29661278-29661297 Msa0247470:intron 30.0%
!! TGTTCTAAAGACACTCTTTA+AGG - chr2_2:29661315-29661334 Msa0247470:CDS 30.0%
TGCCACTAATTAGCATTATA+AGG - chr2_2:29661375-29661394 Msa0247470:CDS 30.0%
CATTATAAGGAAGATGATGA+TGG - chr2_2:29661388-29661407 Msa0247470:CDS 30.0%
!!! TTCTTTTAGGTTGAATGTGT+AGG + chr2_2:29661446-29661465 None:intergenic 30.0%
ATTCAAGAACTAACTATTGG+AGG + chr2_2:29661515-29661534 None:intergenic 30.0%
! GTAAATGAGACAAGTTTTCA+TGG + chr2_2:29661617-29661636 None:intergenic 30.0%
!! TTGTTGTAGATTTGTTGATG+AGG + chr2_2:29661948-29661967 None:intergenic 30.0%
! TCTTACTAGGTAATTGGTTT+CGG + chr2_2:29662324-29662343 None:intergenic 30.0%
AATTAACCACTACTCTTACT+AGG + chr2_2:29662337-29662356 None:intergenic 30.0%
ATAGACTTAGCAAAGACTAG+TGG - chr2_2:29658343-29658362 Msa0247470:three_prime_UTR 35.0%
!! AATTGCTGGAAGTTGATGAA+AGG - chr2_2:29658513-29658532 Msa0247470:CDS 35.0%
AGTATTCTTATGGAAGGAAG+AGG + chr2_2:29658588-29658607 None:intergenic 35.0%
TATGCCATGTTTGTGTATAC+TGG - chr2_2:29658621-29658640 Msa0247470:CDS 35.0%
TATGATGAAAATCATGGGTG+TGG - chr2_2:29658859-29658878 Msa0247470:CDS 35.0%
GGATTTCCTTGAATTTGATG+GGG + chr2_2:29659036-29659055 None:intergenic 35.0%
GGGATTTCCTTGAATTTGAT+GGG + chr2_2:29659037-29659056 None:intergenic 35.0%
! CTTAAGAGGTATGCTAAATC+TGG - chr2_2:29659331-29659350 Msa0247470:intron 35.0%
!!! GTTGTGATTATTTTGAGGCA+AGG + chr2_2:29659423-29659442 None:intergenic 35.0%
!! AAATAATCACAACTTGCTGC+TGG - chr2_2:29659430-29659449 Msa0247470:intron 35.0%
TCGACAACACTACAACATAT+AGG + chr2_2:29659715-29659734 None:intergenic 35.0%
TAGTGTTGTCGATCATCAAT+TGG - chr2_2:29659723-29659742 Msa0247470:intron 35.0%
GCATGTGAGTACATTAATGA+AGG - chr2_2:29660034-29660053 Msa0247470:intron 35.0%
TGTAATGTTTAGATGGTCGA+TGG - chr2_2:29660248-29660267 Msa0247470:intron 35.0%
GTAATGTTTAGATGGTCGAT+GGG - chr2_2:29660249-29660268 Msa0247470:intron 35.0%
! GCGGACAAACTATTAATTTG+AGG - chr2_2:29660639-29660658 Msa0247470:CDS 35.0%
TTAATGCATTGAAAAAGGGG+AGG - chr2_2:29660855-29660874 Msa0247470:intron 35.0%
! TTCCAAAGCACTTCAAACTT+AGG - chr2_2:29660882-29660901 Msa0247470:intron 35.0%
GATAATATATGCAAGGTCTG+GGG - chr2_2:29661032-29661051 Msa0247470:intron 35.0%
!!! CATCTTTTTTGGTTGTGTTC+GGG + chr2_2:29661064-29661083 None:intergenic 35.0%
!!! CCATCTTTTTTGGTTGTGTT+CGG + chr2_2:29661065-29661084 None:intergenic 35.0%
AACACAACCAAAAAAGATGG+AGG - chr2_2:29661065-29661084 Msa0247470:intron 35.0%
!!! ACTTTCACCTCCATCTTTTT+TGG + chr2_2:29661075-29661094 None:intergenic 35.0%
AATGTTTGTGAAAGGAAAGG+TGG - chr2_2:29661151-29661170 Msa0247470:intron 35.0%
ATGTTTGTGAAAGGAAAGGT+GGG - chr2_2:29661152-29661171 Msa0247470:intron 35.0%
ATTGCAATTAACAGGTGCAA+AGG - chr2_2:29661801-29661820 Msa0247470:intron 35.0%
TGTTCATCACAACCTATGAA+GGG - chr2_2:29661843-29661862 Msa0247470:intron 35.0%
!! GATGATGTTAGGTTTGCTAT+TGG + chr2_2:29661902-29661921 None:intergenic 35.0%
!! TGTAGATTTGTTGATGAGGT+AGG + chr2_2:29661944-29661963 None:intergenic 35.0%
! TTTGAGTTTGGTGGAGATAA+TGG + chr2_2:29662010-29662029 None:intergenic 35.0%
! GTAGCTATAGCATTTGAGTT+TGG + chr2_2:29662022-29662041 None:intergenic 35.0%
GGAGCAATGATGATTGTTTA+TGG + chr2_2:29662123-29662142 None:intergenic 35.0%
GAAACACCTCAAATGATTGA+GGG + chr2_2:29662165-29662184 None:intergenic 35.0%
! CAATGAGAGAAAACTTGCTT+TGG - chr2_2:29662198-29662217 Msa0247470:CDS 35.0%
AGAAAAAGATCCTTCTCTCA+AGG - chr2_2:29662243-29662262 Msa0247470:exon 35.0%
AATAATGCTTCCTTGAGAGA+AGG + chr2_2:29662256-29662275 None:intergenic 35.0%
ATGATCTCCATTTGTGAATG+AGG + chr2_2:29662290-29662309 None:intergenic 35.0%
CACTACTCTTACTAGGTAAT+TGG + chr2_2:29662330-29662349 None:intergenic 35.0%
CAATTACCTAGTAAGAGTAG+TGG - chr2_2:29662328-29662347 Msa0247470:five_prime_UTR 35.0%
TAGAGCAGCAATCAACACAA+AGG - chr2_2:29658456-29658475 Msa0247470:CDS 40.0%
TGTATGAATCGAGATCATCG+AGG + chr2_2:29658679-29658698 None:intergenic 40.0%
CAACTTCCCCATCAAATTCA+AGG - chr2_2:29659027-29659046 Msa0247470:intron 40.0%
GGGGATTTCCTTGAATTTGA+TGG + chr2_2:29659038-29659057 None:intergenic 40.0%
GAAGCTTGATGAAGCAAAGA+AGG - chr2_2:29659298-29659317 Msa0247470:intron 40.0%
GAAGGGAAATACACAAGATG+CGG - chr2_2:29659895-29659914 Msa0247470:intron 40.0%
TCATCAAAGAACTGACGAGT+AGG + chr2_2:29659935-29659954 None:intergenic 40.0%
TTGCTACAATCTGAAGGTGA+AGG + chr2_2:29659959-29659978 None:intergenic 40.0%
AATAGAGGACCAATCATCTG+AGG - chr2_2:29660072-29660091 Msa0247470:intron 40.0%
AAAATCTGATCGTGCCCTTA+TGG + chr2_2:29660122-29660141 None:intergenic 40.0%
GGGTGTCAATGGAGAAAATA+TGG - chr2_2:29660269-29660288 Msa0247470:intron 40.0%
ATATGGACAAAAGACAGCCA+AGG - chr2_2:29660286-29660305 Msa0247470:intron 40.0%
TATGGACAAAAGACAGCCAA+GGG - chr2_2:29660287-29660306 Msa0247470:intron 40.0%
AGTTTACCTGCTTACGAACT+GGG + chr2_2:29660361-29660380 None:intergenic 40.0%
TAGTTTACCTGCTTACGAAC+TGG + chr2_2:29660362-29660381 None:intergenic 40.0%
CCTCTCTATTGAGAATGATG+CGG - chr2_2:29660620-29660639 Msa0247470:CDS 40.0%
! AGCCTAAGTTTGAAGTGCTT+TGG + chr2_2:29660887-29660906 None:intergenic 40.0%
CCGAACACAACCAAAAAAGA+TGG - chr2_2:29661062-29661081 Msa0247470:intron 40.0%
!! TAAGGAAGATGATGATGGGA+AGG - chr2_2:29661393-29661412 Msa0247470:CDS 40.0%
GTGTTCATCACAACCTATGA+AGG - chr2_2:29661842-29661861 Msa0247470:intron 40.0%
ATGATTATGGCTCCCTTCAT+AGG + chr2_2:29661858-29661877 None:intergenic 40.0%
!! CTGGTGGAAGTTGATGATTA+TGG + chr2_2:29661871-29661890 None:intergenic 40.0%
!!! TTTGCTATTGGTTTTGCTGC+TGG + chr2_2:29661890-29661909 None:intergenic 40.0%
!! TGTTGAGTGCTGATGATGTT+AGG + chr2_2:29661913-29661932 None:intergenic 40.0%
!! GATTTGTTGATGAGGTAGGA+AGG + chr2_2:29661940-29661959 None:intergenic 40.0%
! GAGATAATGGTGAAGAGAAG+AGG + chr2_2:29661997-29662016 None:intergenic 40.0%
! GCTATAGCATTTGAGTTTGG+TGG + chr2_2:29662019-29662038 None:intergenic 40.0%
AGTGTAATTGTAGGGCATGT+TGG + chr2_2:29662058-29662077 None:intergenic 40.0%
GGTAACCTTGGAATGGAAAA+GGG + chr2_2:29662144-29662163 None:intergenic 40.0%
CAAATGATTGAGGGTAACCT+TGG + chr2_2:29662156-29662175 None:intergenic 40.0%
AGGTTACCCTCAATCATTTG+AGG - chr2_2:29662156-29662175 Msa0247470:CDS 40.0%
GGAAACACCTCAAATGATTG+AGG + chr2_2:29662166-29662185 None:intergenic 40.0%
TCCATTTGTGAATGAGGACA+TGG + chr2_2:29662284-29662303 None:intergenic 40.0%
GTTCCATCAACTGAATCTGC+TGG - chr2_2:29658375-29658394 Msa0247470:exon 45.0%
GCTGCTCTATAGTTCATGTG+AGG + chr2_2:29658445-29658464 None:intergenic 45.0%
AACTGACGAGTAGGTAACAC+TGG + chr2_2:29659926-29659945 None:intergenic 45.0%
! AGGACCAATCATCTGAGGTT+GGG - chr2_2:29660077-29660096 Msa0247470:intron 45.0%
! GAGAGCTAGAGTGTCCATAA+GGG - chr2_2:29660105-29660124 Msa0247470:intron 45.0%
AGCCTACTATCGTTGCAGTA+TGG - chr2_2:29660325-29660344 Msa0247470:intron 45.0%
GCCTACTATCGTTGCAGTAT+GGG - chr2_2:29660326-29660345 Msa0247470:intron 45.0%
CCTACTATCGTTGCAGTATG+GGG - chr2_2:29660327-29660346 Msa0247470:intron 45.0%
CCGCATCATTCTCAATAGAG+AGG + chr2_2:29660623-29660642 None:intergenic 45.0%
ATTGTAGGGCATGTTGGAGA+TGG + chr2_2:29662052-29662071 None:intergenic 45.0%
! TTGCTCCCTTTTCCATTCCA+AGG - chr2_2:29662136-29662155 Msa0247470:CDS 45.0%
GGGTAACCTTGGAATGGAAA+AGG + chr2_2:29662145-29662164 None:intergenic 45.0%
GATTGAGGGTAACCTTGGAA+TGG + chr2_2:29662151-29662170 None:intergenic 45.0%
GCCATGTCCTCATTCACAAA+TGG - chr2_2:29662280-29662299 Msa0247470:five_prime_UTR 45.0%
CAGCCAGCAGATTCAGTTGA+TGG + chr2_2:29658381-29658400 None:intergenic 50.0%
GATGGGAAAATCATGCAGCC+AGG - chr2_2:29658964-29658983 Msa0247470:intron 50.0%
! GAGGACCAATCATCTGAGGT+TGG - chr2_2:29660076-29660095 Msa0247470:intron 50.0%
TGCACCCAACCTCAGATGAT+TGG + chr2_2:29660084-29660103 None:intergenic 50.0%
! AATCATCTGAGGTTGGGTGC+AGG - chr2_2:29660083-29660102 Msa0247470:intron 50.0%
! GGAGAGCTAGAGTGTCCATA+AGG - chr2_2:29660104-29660123 Msa0247470:intron 50.0%
AGATGGTCGATGGGTGTCAA+TGG - chr2_2:29660258-29660277 Msa0247470:intron 50.0%
GATAGTAGGCTCGTGGACAA+GGG + chr2_2:29660316-29660335 None:intergenic 50.0%
CCCCATACTGCAACGATAGT+AGG + chr2_2:29660330-29660349 None:intergenic 50.0%
TTCATGCCCAGTTCGTAAGC+AGG - chr2_2:29660352-29660371 Msa0247470:intron 50.0%
!!! GCTATTGGTTTTGCTGCTGG+TGG + chr2_2:29661887-29661906 None:intergenic 50.0%
GATCCTCTCCCACTGTACAA+CGG - chr2_2:29662375-29662394 Msa0247470:five_prime_UTR 50.0%
CGATAGTAGGCTCGTGGACA+AGG + chr2_2:29660317-29660336 None:intergenic 55.0%
CTGCAACGATAGTAGGCTCG+TGG + chr2_2:29660323-29660342 None:intergenic 55.0%
GGAAAGGTGGGAAGCGAACT+AGG - chr2_2:29661164-29661183 Msa0247470:intron 55.0%
ACACCGTTGTACAGTGGGAG+AGG + chr2_2:29662381-29662400 None:intergenic 55.0%
TCGTGGACAAGGGTTGCCCT+TGG + chr2_2:29660306-29660325 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 29658338 29662402 29658338 ID=Msa0247470;Name=Msa0247470
chr2_2 mRNA 29658338 29662402 29658338 ID=Msa0247470-mRNA-1;Parent=Msa0247470;Name=Msa0247470-mRNA-1;_AED=0.03;_eAED=0.03;_QI=150|1|1|1|1|1|6|48|426
chr2_2 exon 29658338 29658925 29658338 ID=Msa0247470-mRNA-1:exon:10532;Parent=Msa0247470-mRNA-1
chr2_2 exon 29660367 29660480 29660367 ID=Msa0247470-mRNA-1:exon:10531;Parent=Msa0247470-mRNA-1
chr2_2 exon 29660593 29660844 29660593 ID=Msa0247470-mRNA-1:exon:10530;Parent=Msa0247470-mRNA-1
chr2_2 exon 29661289 29661459 29661289 ID=Msa0247470-mRNA-1:exon:10529;Parent=Msa0247470-mRNA-1
chr2_2 exon 29661675 29661758 29661675 ID=Msa0247470-mRNA-1:exon:10528;Parent=Msa0247470-mRNA-1
chr2_2 exon 29662133 29662402 29662133 ID=Msa0247470-mRNA-1:exon:10527;Parent=Msa0247470-mRNA-1
chr2_2 five_prime_UTR 29662253 29662402 29662253 ID=Msa0247470-mRNA-1:five_prime_utr;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29662133 29662252 29662133 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29661675 29661758 29661675 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29661289 29661459 29661289 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29660593 29660844 29660593 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29660367 29660480 29660367 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 CDS 29658386 29658925 29658386 ID=Msa0247470-mRNA-1:cds;Parent=Msa0247470-mRNA-1
chr2_2 three_prime_UTR 29658338 29658385 29658338 ID=Msa0247470-mRNA-1:three_prime_utr;Parent=Msa0247470-mRNA-1
Gene Sequence

>Msa0247470

ATGGAAGAAAAAGTTCAAACAATGAAATTGCTGGAAGTTGATGAAAGGCCACCATATGATAATAACAATAAAGATCTACAAAACAAGATCGATGAATCCTCTTCCTTCCATAAGAATACTCCAGGATCTACTCTTTTATTAAGTTTGAGCCTTAATAACACAACACAACTTCCCCATCAAATTCAAGGAAATCCCCAAATCCAAATTGGCAATATACGAGTGAAGCTTGATGAAGCAAAGAAGGAGAATGAAAACTTAAGAGGTATGCTAAATCTGGTCAACGATCGATGCAATGTTCTTCAGAATCGTTTACTTTTGGCTATGCATATGCATCAATCATCTTCCTTGCCTCAAAATAATCACAACTTGCTGCTGAAGGGAAATACACAAGATGCGGAGAAACCAGTGTTACCTACTCGTCAGTTCTTTGATGAGCCTTCACCTTCAGATTGTAGCAAAAATAATGGATTTGCCATAGTGGAAAACAATGAAAACAACATGGGAAGAAATTTTGCATGTGAGTACATTAATGAAGGTGAAATAAATAGCAAAATAGAGGACCAATCATCTGAGGTTGGGTGCAGGAGAGCTAGAGTGTCCATAAGGGCACGATCAGATTTTGCATTTATGGTCGATGGGTGTCAATGGAGAAAATATGGACAAAAGACAGCCAAGGGCAACCCTTGTCCACGAGCCTACTATCGTTGCAGTATGGGGACTTCATGCCCAGTTCGTAAGCAGGTGCAAAGGTGTTTCAAAGATGAGAGTGTGTTCATCACAACCTATGAAGGGAGCCATAATCATCAACTTCCACCAGCAGCAAAACCAATAGCAAACCTAACATCATCAGCACTCAACACATTCCTTCCTACCTCATCAACAAATCTACAACAATATGGCAACAATCTAACAAACACATTCCTCTTCTCTTCACCATTATCTCCACCAAACTCAAATGCTATAGCTACTTTCTCTCCATCTCCAACATGCCCTACAATTACACTTGATTTTACACTTCCTCCAAGTAACTATTTACAATTCAAAAACCATAAACAATCATCATTGCTCCCTTTTCCATTCCAAGGTTACCCTCAATCATTTGAGGTGTTTCCAAATATGATAAACAATGAGAGAAAACTTGCTTTGGTTGATGTTGTCAGTGAAGCTTTAGAAAAAGATCCTTCTCTCAAGGAAGCATTATTTTCAGCCATGTCCTCATTCACAAATGGAGATCATTCAAATAATAACCGAAACCAATTACCTAGTAAGAGTAGTGGTTAA
Protein sequence

>Msa0247470

MEEKVQTMKLLEVDERPPYDNNNKDLQNKIDESSSFHKNTPGSTLLLSLSLNNTTQLPHQIQGNPQIQIGNIRVKLDEAKKENENLRGMLNLVNDRCNVLQNRLLLAMHMHQSSSLPQNNHNLLLKGNTQDAEKPVLPTRQFFDEPSPSDCSKNNGFAIVENNENNMGRNFACEYINEGEINSKIEDQSSEVGCRRARVSIRARSDFAFMVDGCQWRKYGQKTAKGNPCPRAYYRCSMGTSCPVRKQVQRCFKDESVFITTYEGSHNHQLPPAAKPIANLTSSALNTFLPTSSTNLQQYGNNLTNTFLFSSPLSPPNSNAIATFSPSPTCPTITLDFTLPPSNYLQFKNHKQSSLLPFPFQGYPQSFEVFPNMINNERKLALVDVVSEALEKDPSLKEALFSAMSSFTNGDHSNNNRNQLPSKSSG*