AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449470 MtrunA17_Chr4g0075201 93.320 494 26 6 1 489 7 498 0.0 830
Msa1449470 MtrunA17_Chr2g0288871 56.000 500 156 20 15 470 53 532 1.29e-139 413
Msa1449470 MtrunA17_Chr2g0322391 49.614 518 195 16 28 488 81 589 2.97e-129 388
Msa1449470 MtrunA17_Chr3g0099641 41.538 455 207 11 28 467 34 444 4.57e-101 311
Msa1449470 MtrunA17_Chr7g0270831 39.914 466 188 15 35 478 17 412 2.14e-81 259
Msa1449470 MtrunA17_Chr5g0419241 97.196 107 3 0 182 288 1 107 1.82e-72 224
Msa1449470 MtrunA17_Chr4g0025061 51.406 249 102 4 232 475 334 568 1.04e-69 233
Msa1449470 MtrunA17_Chr4g0025061 32.075 159 96 4 30 183 57 208 9.84e-12 67.4
Msa1449470 MtrunA17_Chr4g0024391 55.042 238 98 4 236 468 207 440 4.42e-69 228
Msa1449470 MtrunA17_Chr4g0025051 56.846 241 101 3 236 474 313 552 1.34e-68 230
Msa1449470 MtrunA17_Chr2g0302391 33.596 381 215 9 111 483 68 418 9.94e-53 184
Msa1449470 MtrunA17_Chr7g0216891 31.158 475 197 16 102 468 31 483 3.63e-48 174
Msa1449470 MtrunA17_Chr4g0060271 37.209 344 164 8 88 379 46 389 1.77e-43 162
Msa1449470 MtrunA17_Chr4g0051181 84.416 77 12 0 242 318 227 303 4.16e-42 156
Msa1449470 MtrunA17_Chr4g0001361 33.190 232 97 8 233 464 137 310 9.43e-29 115
Msa1449470 MtrunA17_Chr2g0333851 27.778 360 157 13 107 464 51 309 2.65e-27 111
Msa1449470 MtrunA17_Chr1g0150281 33.051 236 128 7 98 332 8 214 6.30e-27 110
Msa1449470 MtrunA17_Chr1g0150271 32.599 227 95 6 110 334 7 177 2.02e-26 107
Msa1449470 MtrunA17_Chr3g0136961 27.419 372 165 9 100 467 15 285 2.14e-25 105
Msa1449470 MtrunA17_Chr3g0079691 48.039 102 52 1 219 320 456 556 1.57e-24 107
Msa1449470 MtrunA17_Chr3g0079691 41.935 93 52 2 227 319 249 339 1.09e-14 76.6
Msa1449470 MtrunA17_Chr3g0101131 43.182 132 60 4 241 358 398 528 3.85e-24 105
Msa1449470 MtrunA17_Chr3g0101131 49.180 61 29 2 258 318 243 301 9.32e-12 67.4
Msa1449470 MtrunA17_Chr3g0106491 54.430 79 35 1 240 318 347 424 4.93e-24 105
Msa1449470 MtrunA17_Chr3g0106491 38.298 94 52 3 227 320 171 258 5.15e-13 71.2
Msa1449470 MtrunA17_Chr5g0445311 55.000 80 35 1 241 320 347 425 5.84e-24 105
Msa1449470 MtrunA17_Chr5g0445311 50.820 61 28 2 258 318 200 258 2.52e-12 68.9
Msa1449470 MtrunA17_Chr7g0243891 42.029 138 63 4 235 369 351 474 1.18e-23 104
Msa1449470 MtrunA17_Chr7g0243891 46.575 73 32 3 246 318 209 274 1.54e-11 66.6
Msa1449470 MtrunA17_Chr5g0432161 52.381 84 39 1 237 320 400 482 3.75e-23 102
Msa1449470 MtrunA17_Chr5g0432161 43.590 78 42 2 258 335 244 319 3.12e-13 72.0
Msa1449470 MtrunA17_Chr5g0447701 44.643 112 59 3 225 335 218 327 4.04e-23 101
Msa1449470 MtrunA17_Chr4g0004911 53.165 79 36 1 240 318 318 395 4.08e-23 102
Msa1449470 MtrunA17_Chr4g0004911 41.333 75 41 2 244 318 172 243 1.59e-13 72.8
Msa1449470 MtrunA17_Chr2g0294231 50.000 82 40 1 242 323 187 267 4.30e-23 100
Msa1449470 MtrunA17_Chr1g0150801 52.703 74 34 1 243 316 87 159 5.44e-23 95.5
Msa1449470 MtrunA17_Chr7g0218081 51.190 84 40 1 237 320 389 471 7.03e-23 101
Msa1449470 MtrunA17_Chr7g0218081 52.459 61 27 2 258 318 228 286 8.16e-13 70.5
Msa1449470 MtrunA17_Chr7g0270191 46.316 95 46 2 242 336 129 218 7.41e-23 98.6
Msa1449470 MtrunA17_Chr7g0224801 52.113 71 33 1 247 317 82 151 2.49e-22 94.0
Msa1449470 MtrunA17_Chr5g0418191 50.617 81 39 1 240 320 302 381 5.55e-22 98.2
Msa1449470 MtrunA17_Chr5g0418191 37.615 109 64 3 258 366 151 255 8.80e-13 70.1
Msa1449470 MtrunA17_Chr7g0249311 51.250 80 38 1 241 320 375 453 9.63e-22 98.6
Msa1449470 MtrunA17_Chr7g0249311 48.333 60 29 2 259 318 180 237 5.22e-11 65.1
Msa1449470 MtrunA17_Chr7g0249301 51.250 80 38 1 241 320 275 353 1.39e-21 96.3
Msa1449470 MtrunA17_Chr7g0249301 46.154 65 33 2 254 318 58 120 4.39e-11 64.7
Msa1449470 MtrunA17_Chr4g0042831 46.316 95 44 2 226 320 252 339 1.87e-21 96.3
Msa1449470 MtrunA17_Chr1g0194481 51.948 77 36 1 242 318 142 217 2.58e-21 94.7
Msa1449470 MtrunA17_Chr8g0386241 45.570 79 42 1 243 321 88 165 7.03e-21 90.1
Msa1449470 MtrunA17_Chr4g0051881 44.554 101 49 2 245 339 165 264 1.04e-20 93.2
Msa1449470 MtrunA17_Chr4g0068541 50.649 77 37 1 242 318 192 267 2.32e-20 93.2
Msa1449470 MtrunA17_Chr5g0418781 47.561 82 42 1 234 315 100 180 4.78e-20 90.1
Msa1449470 MtrunA17_Chr7g0263531 41.489 94 54 1 225 318 328 420 5.03e-20 92.4
Msa1449470 MtrunA17_Chr7g0263531 50.000 60 28 2 259 318 190 247 1.01e-11 67.0
Msa1449470 MtrunA17_Chr8g0383251 45.946 74 39 1 245 318 95 167 1.18e-19 86.7
Msa1449470 MtrunA17_Chr7g0270051 52.703 74 32 1 258 331 53 123 2.40e-19 87.8
Msa1449470 MtrunA17_Chr4g0074001 50.633 79 36 2 242 318 343 420 2.63e-19 90.9
Msa1449470 MtrunA17_Chr5g0443131 50.000 74 36 1 245 318 144 216 1.12e-18 86.7
Msa1449470 MtrunA17_Chr7g0248721 47.500 80 40 2 237 316 120 197 1.42e-18 84.3
Msa1449470 MtrunA17_Chr3g0129361 48.000 75 38 1 241 315 127 200 1.62e-18 84.3
Msa1449470 MtrunA17_Chr1g0147881 48.649 74 38 0 242 315 246 319 1.84e-18 86.7
Msa1449470 MtrunA17_Chr8g0335811 43.902 82 45 1 234 315 130 210 2.50e-18 84.0
Msa1449470 MtrunA17_Chr3g0129321 47.297 74 38 1 245 318 105 177 4.19e-18 82.8
Msa1449470 MtrunA17_Chr5g0401821 48.684 76 39 0 242 317 183 258 6.65e-18 84.0
Msa1449470 MtrunA17_Chr4g0053281 48.101 79 41 0 242 320 250 328 7.54e-18 84.7
Msa1449470 MtrunA17_Chr8g0379461 52.113 71 29 1 258 328 76 141 1.43e-17 82.4
Msa1449470 MtrunA17_Chr2g0319961 39.623 106 50 2 227 318 35 140 2.55e-17 82.0
Msa1449470 MtrunA17_Chr8g0348311 52.113 71 34 0 258 328 155 225 3.01e-17 82.8
Msa1449470 MtrunA17_Chr8g0383351 47.368 76 38 2 241 315 142 216 4.31e-17 80.5
Msa1449470 MtrunA17_Chr7g0228941 47.887 71 37 0 245 315 39 109 4.46e-17 77.4
Msa1449470 MtrunA17_Chr1g0188941 41.667 84 48 1 235 318 28 110 4.83e-17 77.0
Msa1449470 MtrunA17_Chr1g0184691 52.459 61 29 0 259 319 294 354 6.73e-17 82.0
Msa1449470 MtrunA17_Chr2g0284321 51.613 62 30 0 258 319 35 96 8.30e-17 79.3
Msa1449470 MtrunA17_Chr4g0071521 47.297 74 39 0 242 315 229 302 8.41e-17 81.3
Msa1449470 MtrunA17_Chr2g0293481 48.571 70 36 0 259 328 268 337 1.29e-16 82.4
Msa1449470 MtrunA17_Chr3g0139021 45.946 74 40 0 242 315 298 371 5.60e-16 79.7
Msa1449470 MtrunA17_Chr2g0289181 34.074 135 79 3 253 377 9 143 7.72e-16 77.0
Msa1449470 MtrunA17_Chr7g0248691 37.607 117 71 2 214 328 108 224 9.02e-16 78.6
Msa1449470 MtrunA17_Chr6g0485431 48.000 75 37 2 245 318 141 214 1.19e-14 75.5
Msa1449470 MtrunA17_Chr7g0238921 38.318 107 50 2 231 322 82 187 1.25e-14 74.7
Msa1449470 MtrunA17_Chr8g0387501 44.286 70 39 0 259 328 130 199 1.37e-14 73.9
Msa1449470 MtrunA17_Chr1g0189391 43.056 72 39 2 245 315 84 154 1.05e-13 72.4
Msa1449470 MtrunA17_Chr1g0202461 43.421 76 43 0 259 334 262 337 1.85e-13 72.0
Msa1449470 MtrunA17_Chr3g0126391 53.333 60 26 1 257 314 132 191 5.39e-13 70.1
Msa1449470 MtrunA17_Chr5g0432071 38.462 78 46 2 258 335 104 179 1.39e-12 67.0
Msa1449470 MtrunA17_Chr8g0347251 47.826 69 34 1 257 323 135 203 2.00e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449470 AT1G62300.1 50.688 509 186 17 4 467 50 538 2.26e-125 377
Msa1449470 AT4G04450.1 47.773 494 189 15 28 467 25 503 4.21e-124 372
Msa1449470 AT4G22070.4 48.054 514 169 14 27 467 26 514 8.05e-113 344
Msa1449470 AT4G22070.3 48.054 514 169 14 27 467 26 514 8.05e-113 344
Msa1449470 AT4G22070.2 48.054 514 169 14 27 467 26 514 8.05e-113 344
Msa1449470 AT4G22070.1 48.054 514 169 14 27 467 26 514 8.05e-113 344
Msa1449470 AT4G04450.2 49.761 418 158 13 93 467 1 409 3.69e-110 333
Msa1449470 AT4G01720.1 45.312 384 169 15 97 464 85 443 1.77e-66 222
Msa1449470 AT5G15130.2 44.118 272 115 5 230 469 157 423 8.79e-50 178
Msa1449470 AT5G15130.1 44.118 272 115 5 230 469 199 465 1.00e-49 179
Msa1449470 AT1G68150.1 42.194 237 110 6 114 339 96 316 5.24e-47 167
Msa1449470 AT1G18860.1 42.069 290 107 8 241 469 222 511 5.29e-44 162
Msa1449470 AT1G69810.1 36.364 308 155 10 108 381 22 322 5.53e-38 143
Msa1449470 AT1G69810.2 54.930 142 58 3 241 381 128 264 1.91e-36 137
Msa1449470 AT4G31800.1 35.270 241 125 5 105 336 36 254 2.75e-33 128
Msa1449470 AT1G80840.1 34.034 238 108 7 92 318 7 206 3.97e-31 122
Msa1449470 AT2G25000.1 32.870 216 103 4 107 322 37 210 1.11e-26 109
Msa1449470 AT2G25000.2 33.173 208 97 4 115 322 2 167 2.06e-26 107
Msa1449470 AT2G25000.4 33.173 208 97 4 115 322 2 167 2.06e-26 107
Msa1449470 AT2G25000.3 33.173 208 97 4 115 322 24 189 3.20e-26 107
Msa1449470 AT4G31800.2 55.000 80 36 0 257 336 110 189 3.44e-25 104
Msa1449470 AT2G38470.1 53.086 81 37 1 240 320 344 423 2.70e-24 106
Msa1449470 AT2G38470.1 46.575 73 36 3 246 318 173 242 8.45e-13 70.9
Msa1449470 AT4G26640.5 55.128 78 34 1 241 318 188 264 6.53e-24 103
Msa1449470 AT4G26640.5 38.095 84 50 2 235 318 12 93 4.18e-12 68.2
Msa1449470 AT4G26640.6 55.128 78 34 1 241 318 188 264 6.53e-24 103
Msa1449470 AT4G26640.6 38.095 84 50 2 235 318 12 93 4.18e-12 68.2
Msa1449470 AT4G26640.4 55.128 78 34 1 241 318 188 264 6.53e-24 103
Msa1449470 AT4G26640.4 38.095 84 50 2 235 318 12 93 4.18e-12 68.2
Msa1449470 AT4G26640.3 55.128 78 34 1 241 318 188 264 6.53e-24 103
Msa1449470 AT4G26640.3 38.095 84 50 2 235 318 12 93 4.18e-12 68.2
Msa1449470 AT4G26640.7 55.128 78 34 1 241 318 324 400 1.39e-23 104
Msa1449470 AT4G26640.7 38.095 84 50 2 235 318 148 229 5.91e-12 68.2
Msa1449470 AT4G26640.1 55.128 78 34 1 241 318 292 368 1.77e-23 103
Msa1449470 AT4G26640.1 38.095 84 50 2 235 318 116 197 6.82e-12 68.2
Msa1449470 AT4G26640.2 55.128 78 34 1 241 318 364 440 2.04e-23 103
Msa1449470 AT4G26640.2 38.095 84 50 2 235 318 188 269 6.67e-12 68.2
Msa1449470 AT5G56270.1 50.000 90 44 1 231 320 460 548 2.67e-23 103
Msa1449470 AT5G56270.1 49.180 61 29 2 259 319 274 332 1.95e-11 67.0
Msa1449470 AT5G26170.1 52.778 72 33 1 244 315 99 169 3.91e-23 96.7
Msa1449470 AT2G04880.2 47.674 86 44 1 237 322 262 346 4.37e-23 102
Msa1449470 AT2G04880.2 31.081 148 82 5 256 392 109 247 6.18e-13 71.2
Msa1449470 AT2G04880.1 47.674 86 44 1 237 322 286 370 5.43e-23 102
Msa1449470 AT2G04880.1 47.143 70 34 3 256 324 109 176 1.37e-12 70.1
Msa1449470 AT1G29860.1 46.392 97 48 2 242 338 120 212 6.44e-23 99.0
Msa1449470 AT5G49520.1 51.948 77 36 1 242 318 205 280 6.62e-23 100
Msa1449470 AT5G07100.2 48.276 87 44 1 232 318 115 200 7.99e-23 97.1
Msa1449470 AT5G07100.2 39.189 74 44 1 258 331 24 96 4.07e-11 63.2
Msa1449470 AT5G07100.4 48.276 87 44 1 232 318 115 200 7.99e-23 97.1
Msa1449470 AT5G07100.4 39.189 74 44 1 258 331 24 96 4.07e-11 63.2
Msa1449470 AT5G07100.5 48.276 87 44 1 232 318 115 200 7.99e-23 97.1
Msa1449470 AT5G07100.5 39.189 74 44 1 258 331 24 96 4.07e-11 63.2
Msa1449470 AT5G07100.3 48.276 87 44 1 232 318 115 200 7.99e-23 97.1
Msa1449470 AT5G07100.3 39.189 74 44 1 258 331 24 96 4.07e-11 63.2
Msa1449470 AT1G13960.1 51.190 84 40 1 235 318 386 468 1.15e-22 101
Msa1449470 AT1G13960.1 49.180 61 29 2 258 318 229 287 5.72e-12 68.2
Msa1449470 AT1G13960.2 51.190 84 40 1 235 318 233 315 1.20e-22 99.8
Msa1449470 AT1G13960.2 49.180 61 29 2 258 318 76 134 6.79e-12 67.4
Msa1449470 AT5G07100.1 48.276 87 44 1 232 318 208 293 1.26e-22 98.6
Msa1449470 AT5G07100.1 39.189 74 44 1 258 331 117 189 9.34e-11 63.5
Msa1449470 AT2G03340.1 48.837 86 43 1 235 320 392 476 1.13e-21 98.6
Msa1449470 AT2G03340.1 49.180 61 29 2 258 318 250 308 2.51e-12 69.3
Msa1449470 AT4G18170.1 51.948 77 36 1 242 318 156 231 1.53e-21 95.9
Msa1449470 AT5G13080.1 53.425 73 33 1 245 317 54 125 2.08e-21 90.9
Msa1449470 AT2G47260.1 45.161 93 50 1 234 326 150 241 2.13e-21 95.5
Msa1449470 AT1G64000.1 45.882 85 45 1 232 316 88 171 2.95e-21 92.0
Msa1449470 AT5G41570.1 51.250 80 37 2 238 316 77 155 6.38e-21 90.5
Msa1449470 AT2G46130.1 47.126 87 45 1 230 316 2 87 7.41e-21 88.2
Msa1449470 AT4G31800.3 32.500 200 104 5 105 295 36 213 1.08e-20 91.7
Msa1449470 AT4G26440.1 41.071 112 63 2 231 342 345 453 2.06e-20 94.7
Msa1449470 AT1G55600.1 43.333 90 50 1 231 320 280 368 2.20e-20 94.4
Msa1449470 AT3G62340.1 53.165 79 36 1 245 323 105 182 2.31e-20 91.3
Msa1449470 AT3G01080.1 46.429 84 44 1 235 318 283 365 2.44e-20 93.6
Msa1449470 AT3G01080.1 49.180 61 29 2 258 318 167 225 8.35e-11 64.3
Msa1449470 AT3G01080.2 49.367 79 39 1 240 318 326 403 2.63e-20 94.0
Msa1449470 AT3G01080.2 49.180 61 29 2 258 318 205 263 9.19e-11 64.3
Msa1449470 AT4G30935.1 41.176 119 58 3 225 335 298 412 4.01e-20 93.2
Msa1449470 AT2G30250.1 50.000 80 39 1 241 320 311 389 4.15e-20 92.8
Msa1449470 AT5G46350.1 50.000 74 36 1 245 318 170 242 4.36e-20 91.7
Msa1449470 AT1G69310.4 45.783 83 44 1 233 315 122 203 4.40e-20 90.9
Msa1449470 AT1G69310.2 45.783 83 44 1 233 315 122 203 4.40e-20 90.9
Msa1449470 AT1G69310.1 45.783 83 44 1 233 315 122 203 4.40e-20 90.9
Msa1449470 AT3G01970.1 51.389 72 34 1 244 315 51 121 1.47e-19 85.5
Msa1449470 AT2G23320.1 59.322 59 24 0 259 317 241 299 1.05e-18 87.4
Msa1449470 AT2G30590.1 50.000 82 40 1 242 322 297 378 1.16e-18 88.2
Msa1449470 AT5G64810.1 47.887 71 36 1 245 315 97 166 1.26e-18 84.3
Msa1449470 AT3G58710.1 42.056 107 47 2 227 318 25 131 1.27e-18 86.3
Msa1449470 AT3G58710.2 42.453 106 47 2 227 318 25 130 1.74e-18 85.9
Msa1449470 AT2G44745.1 46.667 75 39 1 241 315 128 201 3.64e-18 83.6
Msa1449470 AT5G28650.1 62.264 53 20 0 263 315 267 319 4.07e-18 85.9
Msa1449470 AT1G29280.1 52.459 61 29 0 258 318 74 134 7.19e-18 84.0
Msa1449470 AT4G39410.1 48.000 75 38 1 241 315 206 279 9.53e-18 84.3
Msa1449470 AT3G04670.1 57.895 57 24 0 259 315 263 319 2.26e-17 83.6
Msa1449470 AT4G24240.1 48.684 76 39 0 242 317 265 340 3.13e-17 83.6
Msa1449470 AT4G31550.2 48.649 74 38 0 242 315 229 302 3.36e-17 83.2
Msa1449470 AT4G31550.1 48.649 74 38 0 242 315 230 303 3.49e-17 83.2
Msa1449470 AT2G24570.1 48.649 74 38 0 242 315 227 300 4.79e-17 82.4
Msa1449470 AT4G01250.1 40.426 94 51 1 240 328 105 198 1.53e-16 80.5
Msa1449470 AT2G37260.3 45.977 87 41 3 253 334 264 349 1.72e-16 81.3
Msa1449470 AT2G37260.3 43.243 74 40 2 259 332 86 157 7.20e-14 73.2
Msa1449470 AT2G37260.2 45.977 87 41 3 253 334 262 347 1.74e-16 81.3
Msa1449470 AT2G37260.2 43.243 74 40 2 259 332 84 155 7.70e-14 73.2
Msa1449470 AT5G52830.1 46.753 77 41 0 252 328 159 235 1.90e-16 81.3
Msa1449470 AT2G34830.1 46.053 76 40 1 244 318 200 275 2.05e-16 81.6
Msa1449470 AT2G34830.2 46.053 76 40 1 244 318 209 284 2.53e-16 81.6
Msa1449470 AT1G30650.1 46.053 76 40 1 244 318 202 277 2.54e-16 81.6
Msa1449470 AT2G37260.1 45.977 87 41 3 253 334 344 429 3.73e-16 80.9
Msa1449470 AT2G37260.1 43.243 74 40 2 259 332 166 237 1.30e-13 73.2
Msa1449470 AT2G46130.2 43.678 87 44 2 230 316 2 83 7.28e-16 73.9
Msa1449470 AT4G23550.1 41.975 81 47 0 253 333 135 215 1.34e-15 78.2
Msa1449470 AT1G69310.3 42.169 83 43 2 233 315 122 199 3.48e-15 76.3
Msa1449470 AT5G43290.1 35.455 110 52 3 215 323 87 178 1.13e-14 74.7
Msa1449470 AT5G45050.2 40.449 89 41 1 258 334 1152 1240 2.00e-13 73.6
Msa1449470 AT5G45050.1 40.449 89 41 1 258 334 1180 1268 2.05e-13 73.6
Msa1449470 AT5G45050.4 40.449 89 41 1 258 334 1041 1129 2.29e-13 73.6
Msa1449470 AT2G21900.1 43.548 62 34 1 257 318 108 168 2.87e-13 69.3
Msa1449470 AT2G46400.1 42.500 80 44 1 237 314 83 162 3.88e-12 67.4
Msa1449470 AT5G24110.1 50.000 60 28 1 257 314 112 171 5.72e-12 67.0
Msa1449470 AT5G45260.1 37.255 102 57 3 234 333 1189 1285 8.03e-12 68.6
Msa1449470 AT2G40740.1 35.849 106 51 3 258 346 173 278 3.10e-11 64.7
Msa1449470 AT2G40740.3 35.849 106 51 3 258 346 182 287 3.51e-11 64.7
Msa1449470 AT3G01080.3 49.180 61 29 2 258 318 205 263 5.22e-11 64.3

Find 130 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGAAGGCCGGAGAATT+TGG 0.150973 tig0032628:+9054 None:intergenic
AAGAATTCTGGCTAGTAAAT+TGG 0.190593 tig0032628:+9264 None:intergenic
TCAGCATTTCCTTCAGCTTT+TGG 0.196513 tig0032628:+10279 None:intergenic
GCTGCTACTGTGTTACTATC+TGG 0.288928 tig0032628:-9319 Msa1449470:CDS
GGGATTACTGTTATTGTTGC+TGG 0.289882 tig0032628:+8895 None:intergenic
GGCATTGGAGGGGTTTAATC+TGG 0.291166 tig0032628:+9883 None:intergenic
GGGAGGAGCAGCACCAATTA+TGG 0.310343 tig0032628:+8916 None:intergenic
GAGGCTGTCCAAGCTGGAAT+TGG 0.315291 tig0032628:+9134 None:intergenic
CTGTTTGGCAAGAGAGCTTA+TGG 0.316403 tig0032628:-8798 Msa1449470:three_prime_UTR
CACTTCCGGTGTTAGTAATA+AGG 0.320090 tig0032628:+10613 None:intergenic
AGAATTCTGGCTAGTAAATT+GGG 0.323046 tig0032628:+9265 None:intergenic
TTCTGAGGTGTGCATGGTTC+AGG 0.327374 tig0032628:+9971 None:intergenic
ATCAGAACTAGTACAACTTT+TGG 0.338708 tig0032628:-9921 Msa1449470:CDS
CACCATTGATTGATCACTTC+CGG 0.338711 tig0032628:+10599 None:intergenic
GGATGCCAATGGCGAAAATA+TGG 0.339559 tig0032628:-9674 Msa1449470:CDS
GGTAGCACTGTTGATGATAT+TGG 0.345701 tig0032628:+8874 None:intergenic
GAAACATCATCAAGTTGTTC+TGG 0.348285 tig0032628:+11074 None:intergenic
CCTGATCATGTTCTGTGGTT+TGG 0.349953 tig0032628:+10189 None:intergenic
GCTGCTATCTCCTCCATAAT+TGG 0.361075 tig0032628:-8929 Msa1449470:CDS
TGCTTGATCAGCGGCATTGG+AGG 0.372243 tig0032628:+9871 None:intergenic
GTAGCACTGTTGATGATATT+GGG 0.374246 tig0032628:+8875 None:intergenic
TTGGGTTCTATAAGTCTGTT+TGG 0.383456 tig0032628:-8813 Msa1449470:three_prime_UTR
GCGAGTGCTGCCGTGAAGTT+TGG 0.385939 tig0032628:+8953 None:intergenic
CGGCATGAATCGTGTAACTA+TGG 0.386196 tig0032628:-11138 Msa1449470:CDS
CTTGGACAGCCTCAAAACTT+TGG 0.392430 tig0032628:-9124 Msa1449470:CDS
GTTCTGTTGCTGTTGCATTA+AGG 0.395721 tig0032628:+10211 None:intergenic
AGAGCAATTAGGAAGAATTC+TGG 0.399082 tig0032628:+9252 None:intergenic
TTACAGATGCACAATGGCTC+TGG 0.402318 tig0032628:-9612 Msa1449470:CDS
AGACTCACTTGTTTGCGAAC+GGG 0.403359 tig0032628:+9584 None:intergenic
AGTTCTGATGCGTCTGGATC+AGG 0.404046 tig0032628:+9935 None:intergenic
TGATCATCCACTTCTGCTGC+TGG 0.410282 tig0032628:+10001 None:intergenic
CCTGTTGAGTTGGATGCAAC+TGG 0.412568 tig0032628:+9008 None:intergenic
TACCATGTGGGCTTCTGATC+GGG 0.414425 tig0032628:+9805 None:intergenic
TTCAGGAAGCTAGAAGCTCT+TGG 0.420968 tig0032628:-8832 Msa1449470:three_prime_UTR
CAACATCTTGAGACATTTGA+AGG 0.433132 tig0032628:+9041 None:intergenic
CTGTAATATGCGCGAGGACA+AGG 0.435386 tig0032628:+9629 None:intergenic
TATCTGTAGTGTCGCGTGTA+AGG 0.437510 tig0032628:+9176 None:intergenic
CCAGTTGCATCCAACTCAAC+AGG 0.445863 tig0032628:-9008 Msa1449470:CDS
TCAGGAAGCTAGAAGCTCTT+GGG 0.449470 tig0032628:-8831 Msa1449470:three_prime_UTR
AGGCTGTCCAAGCTGGAATT+GGG 0.454087 tig0032628:+9135 None:intergenic
TCCTAATTGCTCTTCTAGCA+TGG 0.455237 tig0032628:-9242 Msa1449470:CDS
TATAACCAATCCAAATTCTC+CGG 0.471393 tig0032628:-9064 Msa1449470:CDS
GTGTGCATGGTTCAGGCTCT+TGG 0.473309 tig0032628:+9978 None:intergenic
GAAATTGACCTGTTCAGTCT+TGG 0.478191 tig0032628:+10539 None:intergenic
GCTGCGGTGGTCGATGCCAT+TGG 0.480782 tig0032628:+9343 None:intergenic
CTGCTACTGTGTTACTATCT+GGG 0.480934 tig0032628:-9318 Msa1449470:CDS
GCTTGATCAGCGGCATTGGA+GGG 0.485891 tig0032628:+9872 None:intergenic
GAAGTTTGGATCCGCGGTGA+TGG 0.486096 tig0032628:+8967 None:intergenic
CAAAACGCAAATAGACTCAT+AGG 0.486782 tig0032628:+8775 None:intergenic
CAGCCTCAAAACTTTGGATC+AGG 0.489452 tig0032628:-9118 Msa1449470:CDS
GGATTACTGTTATTGTTGCT+GGG 0.489961 tig0032628:+8896 None:intergenic
TCTAGTGTCACAGTAGGAAA+TGG 0.493043 tig0032628:+9199 None:intergenic
TGTACTAGTTCTGATGCGTC+TGG 0.496058 tig0032628:+9929 None:intergenic
TAAGCGTGCCAAGACTGAAC+AGG 0.497408 tig0032628:-10547 Msa1449470:intron
ATTTGCTTGATCAGCGGCAT+TGG 0.498345 tig0032628:+9868 None:intergenic
GGCTCTTTCTGATTGTTCTG+AGG 0.499101 tig0032628:+9956 None:intergenic
GGCATAGCAGCAGGTGGGAG+TGG 0.499597 tig0032628:+9364 None:intergenic
ATCTTGAGACATTTGAAGGC+CGG 0.503538 tig0032628:+9045 None:intergenic
TAGCAGCAGGTGGGAGTGGA+TGG 0.505934 tig0032628:+9368 None:intergenic
GACTCACTTGTTTGCGAACG+GGG 0.507020 tig0032628:+9585 None:intergenic
TCTCCTTCCCAATTCCAGCT+TGG 0.508244 tig0032628:-9142 Msa1449470:CDS
TTTACATTTCAGATTGTCAA+AGG 0.510724 tig0032628:-10076 Msa1449470:intron
CTTGATCAGCGGCATTGGAG+GGG 0.514641 tig0032628:+9873 None:intergenic
CCAAACCACAGAACATGATC+AGG 0.515476 tig0032628:-10189 Msa1449470:intron
ATACCATGTGGGCTTCTGAT+CGG 0.516534 tig0032628:+9804 None:intergenic
ATGAATCTAATAAGCACAAA+CGG 0.517014 tig0032628:-11209 Msa1449470:CDS
GGTCAATGTCAAGTGCTGAT+GGG 0.517767 tig0032628:-9297 Msa1449470:CDS
GGGTCAATGTCAAGTGCTGA+TGG 0.517914 tig0032628:-9298 Msa1449470:CDS
TGATTGTTCTGAGGTGTGCA+TGG 0.520062 tig0032628:+9965 None:intergenic
GCCATGCTAGAAGAGCAATT+AGG 0.522733 tig0032628:+9241 None:intergenic
AAAGGAAAAGCTGAGGAGAA+AGG 0.526527 tig0032628:-10058 Msa1449470:CDS
GAGCAATTGCAAGAGGAGCT+TGG 0.529769 tig0032628:-10320 Msa1449470:CDS
AAAGCTGAGGAGAAAGGAGA+TGG 0.531880 tig0032628:-10052 Msa1449470:CDS
GCATTGGAGGGGTTTAATCT+GGG 0.534841 tig0032628:+9884 None:intergenic
GATGCCATTGGCATAGCAGC+AGG 0.540165 tig0032628:+9355 None:intergenic
GTTTGGATCCGCGGTGATGG+CGG 0.540992 tig0032628:+8970 None:intergenic
GATCAATCAATGGTGGATGA+TGG 0.542601 tig0032628:-10591 Msa1449470:CDS
TTAATCTGGGAAGCTGACTT+CGG 0.543926 tig0032628:+9897 None:intergenic
TCACAATTGCAGATCAATGA+TGG 0.544968 tig0032628:-9695 Msa1449470:intron
TGTGGGCTTCTGATCGGGCA+CGG 0.545553 tig0032628:+9810 None:intergenic
TACAGATGCACAATGGCTCT+GGG 0.545795 tig0032628:-9611 Msa1449470:CDS
AGTGATGATGCCTGTTGAGT+TGG 0.552709 tig0032628:+8998 None:intergenic
CACCGGAAGTGATCAATCAA+TGG 0.556816 tig0032628:-10601 Msa1449470:CDS
CTTCAAATGTCTCAAGATGT+TGG 0.558376 tig0032628:-9040 Msa1449470:CDS
CAGACTCACTTGTTTGCGAA+CGG 0.559552 tig0032628:+9583 None:intergenic
CACAGTAGCAGCAGCTGCGG+TGG 0.562058 tig0032628:+9330 None:intergenic
GAATGGTCCAGCAGCAGAAG+TGG 0.565888 tig0032628:-10008 Msa1449470:CDS
TTCTGCATTTGCTTGATCAG+CGG 0.571955 tig0032628:+9862 None:intergenic
CCCACCTGCTGCTATGCCAA+TGG 0.574594 tig0032628:-9359 Msa1449470:CDS
GAAGGCCGGAGAATTTGGAT+TGG 0.577352 tig0032628:+9059 None:intergenic
CGCGACACTACAGATAATAA+CGG 0.582926 tig0032628:-9169 Msa1449470:CDS
GCCATTGGCATAGCAGCAGG+TGG 0.589340 tig0032628:+9358 None:intergenic
TGTAATATGCGCGAGGACAA+GGG 0.590385 tig0032628:+9630 None:intergenic
CAACTCCTTATTACTAACAC+CGG 0.591469 tig0032628:-10618 Msa1449470:CDS
CAAATGTCTCAAGATGTTGG+AGG 0.596501 tig0032628:-9037 Msa1449470:CDS
AATCACCTGATCATGTTCTG+TGG 0.597094 tig0032628:+10184 None:intergenic
GTGCTTATTAGATTCATCGT+CGG 0.598438 tig0032628:+11215 None:intergenic
ATTATAGGTTCAACGTTGTG+CGG 0.604622 tig0032628:-9434 Msa1449470:intron
CGCATATTACAGATGCACAA+TGG 0.609217 tig0032628:-9618 Msa1449470:CDS
TGCAGAGAACCAAAAGCTGA+AGG 0.611336 tig0032628:-10288 Msa1449470:CDS
CACCGCGGATCCAAACTTCA+CGG 0.616851 tig0032628:-8963 Msa1449470:CDS
TGCCCGATCAGAAGCCCACA+TGG 0.617135 tig0032628:-9807 Msa1449470:intron
CGGAAGTGATCAATCAATGG+TGG 0.617601 tig0032628:-10598 Msa1449470:CDS
TGCCGTGAAGTTTGGATCCG+CGG 0.618501 tig0032628:+8961 None:intergenic
GTCAATGTCAAGTGCTGATG+GGG 0.622615 tig0032628:-9296 Msa1449470:CDS
TGTCCAAGCTGGAATTGGGA+AGG 0.625433 tig0032628:+9139 None:intergenic
TTAGACGGAGCAATTGCAAG+AGG 0.625946 tig0032628:-10327 Msa1449470:intron
GAATTCTGGCTAGTAAATTG+GGG 0.628387 tig0032628:+9266 None:intergenic
GTGGCTAGAAAGTTGATGAA+TGG 0.628570 tig0032628:-10025 Msa1449470:CDS
GTAAGGTCTAGTGTCACAGT+AGG 0.632116 tig0032628:+9193 None:intergenic
TGAGGAGAAAGGAGATGGTG+TGG 0.634590 tig0032628:-10047 Msa1449470:CDS
TTACTGTTATTGTTGCTGGG+AGG 0.638488 tig0032628:+8899 None:intergenic
ATAGGTTCAACGTTGTGCGG+AGG 0.648017 tig0032628:-9431 Msa1449470:intron
TGGTGCTGATGCTGAGAGTG+TGG 0.648731 tig0032628:+9219 None:intergenic
CCATTGGCATAGCAGCAGGT+GGG 0.656238 tig0032628:+9359 None:intergenic
GATTGTCAAAGGAAAAGCTG+AGG 0.658561 tig0032628:-10065 Msa1449470:CDS
AGTGCTTGTGCAATCACTTG+TGG 0.658579 tig0032628:+9088 None:intergenic
AGGAGCAGCACCAATTATGG+AGG 0.660834 tig0032628:+8919 None:intergenic
TAACACAGTAGCAGCAGCTG+CGG 0.661912 tig0032628:+9327 None:intergenic
TAAGCACAAACGGAGCAACA+TGG 0.662166 tig0032628:-11199 Msa1449470:CDS
CTGTTGAGTTGGATGCAACT+GGG 0.664888 tig0032628:+9009 None:intergenic
GTGCATCTGTAATATGCGCG+AGG 0.676244 tig0032628:+9623 None:intergenic
ACTAATATAGTATACCATGT+GGG 0.681722 tig0032628:+9793 None:intergenic
AGATCAATGATGGATGCCAA+TGG 0.705200 tig0032628:-9685 Msa1449470:CDS
AATGAAGATGATCAAAATCG+CGG 0.707922 tig0032628:-11161 Msa1449470:CDS
GGAGAAAGGAGATGGTGTGG+TGG 0.745088 tig0032628:-10044 Msa1449470:CDS
TCAGCGCGGAGATTGCAATG+AGG 0.745989 tig0032628:+11011 None:intergenic
ACTAAGTGCCGCCATCACCG+CGG 0.753006 tig0032628:-8978 Msa1449470:CDS
GAAGATGATCAAAATCGCGG+CGG 0.753200 tig0032628:-11158 Msa1449470:CDS
TTACGTTGACATGGTCAGCG+CGG 0.778275 tig0032628:+10997 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAGAAAGAAAAAA+TGG + tig0032628:9015-9034 None:intergenic 10.0%
!! ATTAGTGAAATAAAAAATAT+TGG - tig0032628:9192-9211 Msa1449470:CDS 10.0%
!! AATGTGAAAATATTTATCAT+AGG - tig0032628:9288-9307 Msa1449470:CDS 15.0%
!! ATGTGAAAATATTTATCATA+GGG - tig0032628:9289-9308 Msa1449470:CDS 15.0%
!! AAAATGAAATTAAACTAAGA+TGG + tig0032628:9479-9498 None:intergenic 15.0%
!!! TTAGTTTAATTTCATTTTAC+AGG - tig0032628:9481-9500 Msa1449470:intron 15.0%
!!! TAGTTTAATTTCATTTTACA+GGG - tig0032628:9482-9501 Msa1449470:intron 15.0%
!!! TGTTTCATTTGAAATATTAT+AGG - tig0032628:10536-10555 Msa1449470:intron 15.0%
!! AGTTACAAAGTAGAAATATA+AGG - tig0032628:9045-9064 Msa1449470:CDS 20.0%
!! AGAAATATAAGGAAAATACT+TGG - tig0032628:9056-9075 Msa1449470:CDS 20.0%
!! AATATTTATCATAGGGATTA+CGG - tig0032628:9296-9315 Msa1449470:CDS 20.0%
!!! AGTTTAATTTCATTTTACAG+GGG - tig0032628:9483-9502 Msa1449470:intron 20.0%
!!! ACACTTGTTAAATTTTTAGA+CGG - tig0032628:9643-9662 Msa1449470:CDS 20.0%
!!! GGTATAAAGATATTTTAAAC+TGG + tig0032628:9843-9862 None:intergenic 20.0%
!!! TCATAATTTCATTTCAAGTT+CGG + tig0032628:9866-9885 None:intergenic 20.0%
!! TTGAAATGAAATTATGAAAC+TGG - tig0032628:9869-9888 Msa1449470:CDS 20.0%
!! ATTAGTTAATTAAATCAGCT+AGG + tig0032628:10267-10286 None:intergenic 20.0%
!!! ATTTTGATAACAACATATGA+AGG - tig0032628:10585-10604 Msa1449470:CDS 20.0%
!!! TTTTGATAACAACATATGAA+GGG - tig0032628:10586-10605 Msa1449470:CDS 20.0%
! ATGAATCTAATAAGCACAAA+CGG - tig0032628:8776-8795 Msa1449470:three_prime_UTR 25.0%
!!! TAAATACGATATTTGGTTTG+GGG + tig0032628:9083-9102 None:intergenic 25.0%
!! GTAAATACGATATTTGGTTT+GGG + tig0032628:9084-9103 None:intergenic 25.0%
! AGTAAATACGATATTTGGTT+TGG + tig0032628:9085-9104 None:intergenic 25.0%
! CAAACAGTAAATACGATATT+TGG + tig0032628:9090-9109 None:intergenic 25.0%
!!! ATAATTTCATTTCAAGTTCG+GGG + tig0032628:9864-9883 None:intergenic 25.0%
!!! CATAATTTCATTTCAAGTTC+GGG + tig0032628:9865-9884 None:intergenic 25.0%
! AATGAAATTATGAAACTGGA+TGG - tig0032628:9873-9892 Msa1449470:CDS 25.0%
! TTTACATTTCAGATTGTCAA+AGG - tig0032628:9909-9928 Msa1449470:CDS 25.0%
! ACTAATATAGTATACCATGT+GGG + tig0032628:10195-10214 None:intergenic 25.0%
!! AAAAATATGCTACTGATTGT+AGG + tig0032628:11135-11154 None:intergenic 25.0%
AATGAAGATGATCAAAATCG+CGG - tig0032628:8824-8843 Msa1449470:three_prime_UTR 30.0%
!! TCATAATTACCTTTGAAGTG+TGG - tig0032628:9222-9241 Msa1449470:CDS 30.0%
TTGATGCAAATATTGTAGAC+TGG - tig0032628:9340-9359 Msa1449470:CDS 30.0%
! ATCAGAACTAGTACAACTTT+TGG - tig0032628:10064-10083 Msa1449470:CDS 30.0%
CACTAATATAGTATACCATG+TGG + tig0032628:10196-10215 None:intergenic 30.0%
GCGAAAATATGGACAAAAAA+TGG - tig0032628:10322-10341 Msa1449470:CDS 30.0%
TATGGACAAAAAATGGCTAA+AGG - tig0032628:10329-10348 Msa1449470:intron 30.0%
! AGAATTCTGGCTAGTAAATT+GGG + tig0032628:10723-10742 None:intergenic 30.0%
! AAGAATTCTGGCTAGTAAAT+TGG + tig0032628:10724-10743 None:intergenic 30.0%
TATAACCAATCCAAATTCTC+CGG - tig0032628:10921-10940 Msa1449470:intron 30.0%
! CAATCAGTAGCATATTTTTC+AGG - tig0032628:11136-11155 Msa1449470:CDS 30.0%
GTGCTTATTAGATTCATCGT+CGG + tig0032628:8773-8792 None:intergenic 35.0%
! GAAACATCATCAAGTTGTTC+TGG + tig0032628:8914-8933 None:intergenic 35.0%
AAAAATGGCTTACGTTGACA+TGG + tig0032628:9000-9019 None:intergenic 35.0%
AAATCACAACCACACTTCAA+AGG + tig0032628:9234-9253 None:intergenic 35.0%
CAACTCCTTATTACTAACAC+CGG - tig0032628:9367-9386 Msa1449470:CDS 35.0%
TCACAATTGCAGATCAATGA+TGG - tig0032628:10290-10309 Msa1449470:CDS 35.0%
! TTTGTCCATATTTTCGCCAT+TGG + tig0032628:10319-10338 None:intergenic 35.0%
ATTATAGGTTCAACGTTGTG+CGG - tig0032628:10551-10570 Msa1449470:CDS 35.0%
! GAATTCTGGCTAGTAAATTG+GGG + tig0032628:10722-10741 None:intergenic 35.0%
AGAGCAATTAGGAAGAATTC+TGG + tig0032628:10736-10755 None:intergenic 35.0%
CAACATCTTGAGACATTTGA+AGG + tig0032628:10947-10966 None:intergenic 35.0%
CTTCAAATGTCTCAAGATGT+TGG - tig0032628:10945-10964 Msa1449470:intron 35.0%
GGATTACTGTTATTGTTGCT+GGG + tig0032628:11092-11111 None:intergenic 35.0%
!! GTAGCACTGTTGATGATATT+GGG + tig0032628:11113-11132 None:intergenic 35.0%
TTGGGTTCTATAAGTCTGTT+TGG - tig0032628:11172-11191 Msa1449470:CDS 35.0%
CAAAACGCAAATAGACTCAT+AGG + tig0032628:11213-11232 None:intergenic 35.0%
! CACTTCCGGTGTTAGTAATA+AGG + tig0032628:9375-9394 None:intergenic 40.0%
! CACCATTGATTGATCACTTC+CGG + tig0032628:9389-9408 None:intergenic 40.0%
!! GATCAATCAATGGTGGATGA+TGG - tig0032628:9394-9413 Msa1449470:CDS 40.0%
GAAATTGACCTGTTCAGTCT+TGG + tig0032628:9449-9468 None:intergenic 40.0%
! TCAGCATTTCCTTCAGCTTT+TGG + tig0032628:9709-9728 None:intergenic 40.0%
! GTTCTGTTGCTGTTGCATTA+AGG + tig0032628:9777-9796 None:intergenic 40.0%
AATCACCTGATCATGTTCTG+TGG + tig0032628:9804-9823 None:intergenic 40.0%
GATTGTCAAAGGAAAAGCTG+AGG - tig0032628:9920-9939 Msa1449470:CDS 40.0%
AAAGGAAAAGCTGAGGAGAA+AGG - tig0032628:9927-9946 Msa1449470:CDS 40.0%
!! GTGGCTAGAAAGTTGATGAA+TGG - tig0032628:9960-9979 Msa1449470:CDS 40.0%
! TTAATCTGGGAAGCTGACTT+CGG + tig0032628:10091-10110 None:intergenic 40.0%
TTCTGCATTTGCTTGATCAG+CGG + tig0032628:10126-10145 None:intergenic 40.0%
!! AGATCAATGATGGATGCCAA+TGG - tig0032628:10300-10319 Msa1449470:CDS 40.0%
CGCATATTACAGATGCACAA+TGG - tig0032628:10367-10386 Msa1449470:intron 40.0%
GACGCATATTATGAGACGAA+AGG + tig0032628:10506-10525 None:intergenic 40.0%
! CTGCTACTGTGTTACTATCT+GGG - tig0032628:10667-10686 Msa1449470:intron 40.0%
TCCTAATTGCTCTTCTAGCA+TGG - tig0032628:10743-10762 Msa1449470:intron 40.0%
TCTAGTGTCACAGTAGGAAA+TGG + tig0032628:10789-10808 None:intergenic 40.0%
CGCGACACTACAGATAATAA+CGG - tig0032628:10816-10835 Msa1449470:intron 40.0%
! TGTCCTGATCCAAAGTTTTG+AGG + tig0032628:10873-10892 None:intergenic 40.0%
ATCTTGAGACATTTGAAGGC+CGG + tig0032628:10943-10962 None:intergenic 40.0%
CAAATGTCTCAAGATGTTGG+AGG - tig0032628:10948-10967 Msa1449470:intron 40.0%
TTACTGTTATTGTTGCTGGG+AGG + tig0032628:11089-11108 None:intergenic 40.0%
GGGATTACTGTTATTGTTGC+TGG + tig0032628:11093-11112 None:intergenic 40.0%
!! GGTAGCACTGTTGATGATAT+TGG + tig0032628:11114-11133 None:intergenic 40.0%
TAAGCACAAACGGAGCAACA+TGG - tig0032628:8786-8805 Msa1449470:three_prime_UTR 45.0%
GAAGATGATCAAAATCGCGG+CGG - tig0032628:8827-8846 Msa1449470:three_prime_UTR 45.0%
CGGCATGAATCGTGTAACTA+TGG - tig0032628:8847-8866 Msa1449470:exon 45.0%
CACCGGAAGTGATCAATCAA+TGG - tig0032628:9384-9403 Msa1449470:CDS 45.0%
CGGAAGTGATCAATCAATGG+TGG - tig0032628:9387-9406 Msa1449470:CDS 45.0%
TTAGACGGAGCAATTGCAAG+AGG - tig0032628:9658-9677 Msa1449470:CDS 45.0%
TGCAGAGAACCAAAAGCTGA+AGG - tig0032628:9697-9716 Msa1449470:intron 45.0%
CCTGATCATGTTCTGTGGTT+TGG + tig0032628:9799-9818 None:intergenic 45.0%
CCAAACCACAGAACATGATC+AGG - tig0032628:9796-9815 Msa1449470:intron 45.0%
AAAGCTGAGGAGAAAGGAGA+TGG - tig0032628:9933-9952 Msa1449470:CDS 45.0%
!! TGATTGTTCTGAGGTGTGCA+TGG + tig0032628:10023-10042 None:intergenic 45.0%
GGCTCTTTCTGATTGTTCTG+AGG + tig0032628:10032-10051 None:intergenic 45.0%
!! TGTACTAGTTCTGATGCGTC+TGG + tig0032628:10059-10078 None:intergenic 45.0%
GCATTGGAGGGGTTTAATCT+GGG + tig0032628:10104-10123 None:intergenic 45.0%
ATTTGCTTGATCAGCGGCAT+TGG + tig0032628:10120-10139 None:intergenic 45.0%
! GACTCGTGCTTTTCTCATAG+TGG + tig0032628:10150-10169 None:intergenic 45.0%
ATACCATGTGGGCTTCTGAT+CGG + tig0032628:10184-10203 None:intergenic 45.0%
GGATGCCAATGGCGAAAATA+TGG - tig0032628:10311-10330 Msa1449470:CDS 45.0%
TGTAATATGCGCGAGGACAA+GGG + tig0032628:10358-10377 None:intergenic 45.0%
TTACAGATGCACAATGGCTC+TGG - tig0032628:10373-10392 Msa1449470:intron 45.0%
TACAGATGCACAATGGCTCT+GGG - tig0032628:10374-10393 Msa1449470:intron 45.0%
AGACTCACTTGTTTGCGAAC+GGG + tig0032628:10404-10423 None:intergenic 45.0%
CAGACTCACTTGTTTGCGAA+CGG + tig0032628:10405-10424 None:intergenic 45.0%
! GCTGCTACTGTGTTACTATC+TGG - tig0032628:10666-10685 Msa1449470:intron 45.0%
!! GGTCAATGTCAAGTGCTGAT+GGG - tig0032628:10688-10707 Msa1449470:intron 45.0%
!! GTCAATGTCAAGTGCTGATG+GGG - tig0032628:10689-10708 Msa1449470:intron 45.0%
GCCATGCTAGAAGAGCAATT+AGG + tig0032628:10747-10766 None:intergenic 45.0%
GTAAGGTCTAGTGTCACAGT+AGG + tig0032628:10795-10814 None:intergenic 45.0%
TATCTGTAGTGTCGCGTGTA+AGG + tig0032628:10812-10831 None:intergenic 45.0%
CTTGGACAGCCTCAAAACTT+TGG - tig0032628:10861-10880 Msa1449470:intron 45.0%
CAGCCTCAAAACTTTGGATC+AGG - tig0032628:10867-10886 Msa1449470:intron 45.0%
AGTGCTTGTGCAATCACTTG+TGG + tig0032628:10900-10919 None:intergenic 45.0%
CATTTGAAGGCCGGAGAATT+TGG + tig0032628:10934-10953 None:intergenic 45.0%
!! CTGTTGAGTTGGATGCAACT+GGG + tig0032628:10979-10998 None:intergenic 45.0%
! AGTGATGATGCCTGTTGAGT+TGG + tig0032628:10990-11009 None:intergenic 45.0%
GCTGCTATCTCCTCCATAAT+TGG - tig0032628:11056-11075 Msa1449470:CDS 45.0%
TTCAGGAAGCTAGAAGCTCT+TGG - tig0032628:11153-11172 Msa1449470:CDS 45.0%
TCAGGAAGCTAGAAGCTCTT+GGG - tig0032628:11154-11173 Msa1449470:CDS 45.0%
CTGTTTGGCAAGAGAGCTTA+TGG - tig0032628:11187-11206 Msa1449470:CDS 45.0%
TTACGTTGACATGGTCAGCG+CGG + tig0032628:8991-9010 None:intergenic 50.0%
TAAGCGTGCCAAGACTGAAC+AGG - tig0032628:9438-9457 Msa1449470:intron 50.0%
GAGCAATTGCAAGAGGAGCT+TGG - tig0032628:9665-9684 Msa1449470:CDS 50.0%
TGAGGAGAAAGGAGATGGTG+TGG - tig0032628:9938-9957 Msa1449470:CDS 50.0%
TGATCATCCACTTCTGCTGC+TGG + tig0032628:9987-10006 None:intergenic 50.0%
!! TTCTGAGGTGTGCATGGTTC+AGG + tig0032628:10017-10036 None:intergenic 50.0%
! AGTTCTGATGCGTCTGGATC+AGG + tig0032628:10053-10072 None:intergenic 50.0%
! GGCATTGGAGGGGTTTAATC+TGG + tig0032628:10105-10124 None:intergenic 50.0%
TACCATGTGGGCTTCTGATC+GGG + tig0032628:10183-10202 None:intergenic 50.0%
CTGTAATATGCGCGAGGACA+AGG + tig0032628:10359-10378 None:intergenic 50.0%
GTGCATCTGTAATATGCGCG+AGG + tig0032628:10365-10384 None:intergenic 50.0%
GACTCACTTGTTTGCGAACG+GGG + tig0032628:10403-10422 None:intergenic 50.0%
CGTTCGCAAACAAGTGAGTC+TGG - tig0032628:10403-10422 Msa1449470:intron 50.0%
ATAGGTTCAACGTTGTGCGG+AGG - tig0032628:10554-10573 Msa1449470:CDS 50.0%
TAACACAGTAGCAGCAGCTG+CGG + tig0032628:10661-10680 None:intergenic 50.0%
!! GGGTCAATGTCAAGTGCTGA+TGG - tig0032628:10687-10706 Msa1449470:intron 50.0%
TCTCCTTCCCAATTCCAGCT+TGG - tig0032628:10843-10862 Msa1449470:intron 50.0%
TGTCCAAGCTGGAATTGGGA+AGG + tig0032628:10849-10868 None:intergenic 50.0%
AGGCTGTCCAAGCTGGAATT+GGG + tig0032628:10853-10872 None:intergenic 50.0%
!!! AGTTTTGAGGCTGTCCAAGC+TGG + tig0032628:10860-10879 None:intergenic 50.0%
GAAGGCCGGAGAATTTGGAT+TGG + tig0032628:10929-10948 None:intergenic 50.0%
!! CCTGTTGAGTTGGATGCAAC+TGG + tig0032628:10980-10999 None:intergenic 50.0%
CCAGTTGCATCCAACTCAAC+AGG - tig0032628:10977-10996 Msa1449470:intron 50.0%
AGGAGCAGCACCAATTATGG+AGG + tig0032628:11069-11088 None:intergenic 50.0%
TCAGCGCGGAGATTGCAATG+AGG + tig0032628:8977-8996 None:intergenic 55.0%
GGAGAAAGGAGATGGTGTGG+TGG - tig0032628:9941-9960 Msa1449470:CDS 55.0%
GAATGGTCCAGCAGCAGAAG+TGG - tig0032628:9977-9996 Msa1449470:CDS 55.0%
GTGTGCATGGTTCAGGCTCT+TGG + tig0032628:10010-10029 None:intergenic 55.0%
! CTTGATCAGCGGCATTGGAG+GGG + tig0032628:10115-10134 None:intergenic 55.0%
! GCTTGATCAGCGGCATTGGA+GGG + tig0032628:10116-10135 None:intergenic 55.0%
! TGCTTGATCAGCGGCATTGG+AGG + tig0032628:10117-10136 None:intergenic 55.0%
CCATTGGCATAGCAGCAGGT+GGG + tig0032628:10629-10648 None:intergenic 55.0%
GATGCCATTGGCATAGCAGC+AGG + tig0032628:10633-10652 None:intergenic 55.0%
!! TGGTGCTGATGCTGAGAGTG+TGG + tig0032628:10769-10788 None:intergenic 55.0%
GAGGCTGTCCAAGCTGGAAT+TGG + tig0032628:10854-10873 None:intergenic 55.0%
GAAGTTTGGATCCGCGGTGA+TGG + tig0032628:11021-11040 None:intergenic 55.0%
CACCGCGGATCCAAACTTCA+CGG - tig0032628:11022-11041 Msa1449470:CDS 55.0%
TGCCGTGAAGTTTGGATCCG+CGG + tig0032628:11027-11046 None:intergenic 55.0%
GGGAGGAGCAGCACCAATTA+TGG + tig0032628:11072-11091 None:intergenic 55.0%
TGTGGGCTTCTGATCGGGCA+CGG + tig0032628:10178-10197 None:intergenic 60.0%
TGCCCGATCAGAAGCCCACA+TGG - tig0032628:10178-10197 Msa1449470:intron 60.0%
TAGCAGCAGGTGGGAGTGGA+TGG + tig0032628:10620-10639 None:intergenic 60.0%
CCCACCTGCTGCTATGCCAA+TGG - tig0032628:10626-10645 Msa1449470:CDS 60.0%
GCCATTGGCATAGCAGCAGG+TGG + tig0032628:10630-10649 None:intergenic 60.0%
! ACTAAGTGCCGCCATCACCG+CGG - tig0032628:11007-11026 Msa1449470:CDS 60.0%
! GTTTGGATCCGCGGTGATGG+CGG + tig0032628:11018-11037 None:intergenic 60.0%
! GCGAGTGCTGCCGTGAAGTT+TGG + tig0032628:11035-11054 None:intergenic 60.0%
GGCATAGCAGCAGGTGGGAG+TGG + tig0032628:10624-10643 None:intergenic 65.0%
GCTGCGGTGGTCGATGCCAT+TGG + tig0032628:10645-10664 None:intergenic 65.0%
CACAGTAGCAGCAGCTGCGG+TGG + tig0032628:10658-10677 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0032628 gene 8765 11242 8765 ID=Msa1449470;Name=Msa1449470
tig0032628 mRNA 8765 11242 8765 ID=Msa1449470-mRNA-1;Parent=Msa1449470;Name=Msa1449470-mRNA-1;_AED=0.05;_eAED=0.05;_QI=11|1|0.83|1|1|1|6|97|489
tig0032628 exon 8765 9449 8765 ID=Msa1449470-mRNA-1:exon:16964;Parent=Msa1449470-mRNA-1
tig0032628 exon 9592 9705 9592 ID=Msa1449470-mRNA-1:exon:16963;Parent=Msa1449470-mRNA-1
tig0032628 exon 9808 10086 9808 ID=Msa1449470-mRNA-1:exon:16962;Parent=Msa1449470-mRNA-1
tig0032628 exon 10190 10345 10190 ID=Msa1449470-mRNA-1:exon:16961;Parent=Msa1449470-mRNA-1
tig0032628 exon 10548 10649 10548 ID=Msa1449470-mRNA-1:exon:16960;Parent=Msa1449470-mRNA-1
tig0032628 exon 11001 11242 11001 ID=Msa1449470-mRNA-1:exon:16959;Parent=Msa1449470-mRNA-1
tig0032628 five_prime_UTR 11232 11242 11232 ID=Msa1449470-mRNA-1:five_prime_utr;Parent=Msa1449470-mRNA-1
tig0032628 CDS 11001 11231 11001 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 CDS 10548 10649 10548 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 CDS 10190 10345 10190 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 CDS 9808 10086 9808 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 CDS 9592 9705 9592 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 CDS 8862 9449 8862 ID=Msa1449470-mRNA-1:cds;Parent=Msa1449470-mRNA-1
tig0032628 three_prime_UTR 8765 8861 8765 ID=Msa1449470-mRNA-1:three_prime_utr;Parent=Msa1449470-mRNA-1
Gene Sequence

>Msa1449470

ATGAATCTAATAAGCACAAACGGAGCAACATGGTGTGATGAGAATGAAAATGAAGATGATCAAAATCGCGGCGGCATGAATCGTGTAACTATGGATGAAGTTGATTTCTTCTCAAATGATAAATCATCATCATCGCCAGAACAACTTGATGATGTTTCAATGAAGAATAATAATAATAATATTAATGAGATTTACGATCCTCATTGCAATCTCCGCGCTGACCATGTCAACACTGGTTTACAACTCCTTATTACTAACACCGGAAGTGATCAATCAATGGTGGATGATGGAACTTCAATAAATGCTGAAGATAAGCGTGCCAAGACTGAACAGACGGAGCAATTGCAAGAGGAGCTTGGACGTGTGAATGCAGAGAACCAAAAGCTGAAGGAAATGCTGAGTGACATGAGCAGTAGCTACACAAACTTGCATAAGCGTTTTATGTCCTTAATGCAACAGCAACAGAACCAAACCACAGAACATGATCAGATTGTCAAAGGAAAAGCTGAGGAGAAAGGAGATGGTGTGGTGGCTAGAAAGTTGATGAATGGTCCAGCAGCAGAAGTGGATGATCACCAAGAGCCTGAACCATGCACACCTCAGAACAATCAGAAAGAGCCTGATCCAGACGCATCAGAACTAGTACAACTTTTGGACCGAAGTCAGCTTCCCAGATTAAACCCCTCCAATGCCGCTGATCAAGCAAATGCAGAAGCCACTATGAGAAAAGCACGAGTCTCAGTCCGTGCCCGATCAGAAGCCCACATGATCAATGATGGATGCCAATGGCGAAAATATGGACAAAAAATGGCTAAAGGAAACCCTTGTCCTCGCGCATATTACAGATGCACAATGGCTCTGGGTTGCCCCGTTCGCAAACAAGTTCAACGTTGTGCGGAGGACAGATCAATTTTGATAACAACATATGAAGGGACACATAGCCATCCACTCCCACCTGCTGCTATGCCAATGGCATCGACCACCGCAGCTGCTGCTACTGTGTTACTATCTGGGTCAATGTCAAGTGCTGATGGGGTGATGAACCCCAATTTACTAGCCAGAATTCTTCCTAATTGCTCTTCTAGCATGGCCACACTCTCAGCATCAGCACCATTTCCTACTGTGACACTAGACCTTACACGCGACACTACAGATAATAACGGAAATTCTCCTTCCCAATTCCAGCTTGGACAGCCTCAAAACTTTGGATCAGGACAATTACCACAAGTGATTGCACAAGCACTTTATAACCAATCCAAATTCTCCGGCCTTCAAATGTCTCAAGATGTTGGAGGCTCTTCCCAGTTGCATCCAACTCAACAGGCATCATCACTAAGTGCCGCCATCACCGCGGATCCAAACTTCACGGCAGCACTCGCAGCTGCTATCTCCTCCATAATTGGTGCTGCTCCTCCCAGCAACAATAACAGTAATCCCAATATCATCAACAGTGCTACCTACAATCAGTAG
Protein sequence

>Msa1449470

MNLISTNGATWCDENENEDDQNRGGMNRVTMDEVDFFSNDKSSSSPEQLDDVSMKNNNNNINEIYDPHCNLRADHVNTGLQLLITNTGSDQSMVDDGTSINAEDKRAKTEQTEQLQEELGRVNAENQKLKEMLSDMSSSYTNLHKRFMSLMQQQQNQTTEHDQIVKGKAEEKGDGVVARKLMNGPAAEVDDHQEPEPCTPQNNQKEPDPDASELVQLLDRSQLPRLNPSNAADQANAEATMRKARVSVRARSEAHMINDGCQWRKYGQKMAKGNPCPRAYYRCTMALGCPVRKQVQRCAEDRSILITTYEGTHSHPLPPAAMPMASTTAAAATVLLSGSMSSADGVMNPNLLARILPNCSSSMATLSASAPFPTVTLDLTRDTTDNNGNSPSQFQLGQPQNFGSGQLPQVIAQALYNQSKFSGLQMSQDVGGSSQLHPTQQASSLSAAITADPNFTAALAAAISSIIGAAPPSNNNSNPNIINSATYNQ*