AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1223560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1223560 MtrunA17_Chr4g0075201 93.117 494 26 6 1 488 7 498 0.0 830
Msa1223560 MtrunA17_Chr2g0288871 56.112 499 156 19 15 469 53 532 4.18e-140 414
Msa1223560 MtrunA17_Chr2g0322391 49.807 518 193 16 28 487 81 589 6.75e-129 387
Msa1223560 MtrunA17_Chr3g0099641 41.850 454 206 12 28 466 34 444 2.27e-101 311
Msa1223560 MtrunA17_Chr7g0270831 40.129 466 186 16 35 477 17 412 2.15e-81 259
Msa1223560 MtrunA17_Chr5g0419241 97.196 107 3 0 181 287 1 107 1.96e-72 224
Msa1223560 MtrunA17_Chr4g0025061 51.406 249 102 4 231 474 334 568 1.13e-69 233
Msa1223560 MtrunA17_Chr4g0025061 36.792 106 62 2 82 182 103 208 1.16e-11 67.0
Msa1223560 MtrunA17_Chr4g0024391 55.042 238 98 4 235 467 207 440 4.31e-69 228
Msa1223560 MtrunA17_Chr4g0025051 56.846 241 101 3 235 473 313 552 1.29e-68 230
Msa1223560 MtrunA17_Chr2g0302391 33.596 381 215 9 110 482 68 418 1.05e-52 184
Msa1223560 MtrunA17_Chr7g0216891 31.158 475 197 16 101 467 31 483 3.78e-48 174
Msa1223560 MtrunA17_Chr4g0060271 37.209 344 164 8 87 378 46 389 1.60e-43 162
Msa1223560 MtrunA17_Chr4g0051181 84.416 77 12 0 241 317 227 303 4.09e-42 156
Msa1223560 MtrunA17_Chr4g0001361 33.190 232 97 8 232 463 137 310 9.61e-29 115
Msa1223560 MtrunA17_Chr2g0333851 27.778 360 157 13 106 463 51 309 2.60e-27 111
Msa1223560 MtrunA17_Chr1g0150281 33.051 236 128 7 97 331 8 214 5.83e-27 110
Msa1223560 MtrunA17_Chr1g0150271 32.599 227 95 6 109 333 7 177 2.47e-26 107
Msa1223560 MtrunA17_Chr3g0136961 27.419 372 165 9 99 466 15 285 2.51e-25 105
Msa1223560 MtrunA17_Chr3g0079691 48.039 102 52 1 218 319 456 556 1.61e-24 107
Msa1223560 MtrunA17_Chr3g0079691 41.935 93 52 2 226 318 249 339 1.13e-14 76.6
Msa1223560 MtrunA17_Chr3g0101131 43.182 132 60 4 240 357 398 528 3.92e-24 105
Msa1223560 MtrunA17_Chr3g0101131 49.180 61 29 2 257 317 243 301 9.70e-12 67.4
Msa1223560 MtrunA17_Chr3g0106491 54.430 79 35 1 239 317 347 424 4.75e-24 105
Msa1223560 MtrunA17_Chr3g0106491 38.298 94 52 3 226 319 171 258 4.82e-13 71.2
Msa1223560 MtrunA17_Chr5g0445311 55.000 80 35 1 240 319 347 425 5.63e-24 105
Msa1223560 MtrunA17_Chr5g0445311 50.820 61 28 2 257 317 200 258 2.51e-12 68.9
Msa1223560 MtrunA17_Chr7g0243891 42.029 138 63 4 234 368 351 474 1.19e-23 103
Msa1223560 MtrunA17_Chr7g0243891 46.575 73 32 3 245 317 209 274 1.56e-11 66.6
Msa1223560 MtrunA17_Chr5g0432161 52.381 84 39 1 236 319 400 482 3.68e-23 102
Msa1223560 MtrunA17_Chr5g0432161 43.590 78 42 2 257 334 244 319 3.13e-13 72.0
Msa1223560 MtrunA17_Chr5g0447701 44.643 112 59 3 224 334 218 327 3.86e-23 101
Msa1223560 MtrunA17_Chr4g0004911 53.165 79 36 1 239 317 318 395 4.12e-23 102
Msa1223560 MtrunA17_Chr4g0004911 41.333 75 41 2 243 317 172 243 1.60e-13 72.8
Msa1223560 MtrunA17_Chr2g0294231 50.000 82 40 1 241 322 187 267 4.15e-23 100
Msa1223560 MtrunA17_Chr1g0150801 52.703 74 34 1 242 315 87 159 5.57e-23 95.5
Msa1223560 MtrunA17_Chr7g0218081 51.190 84 40 1 236 319 389 471 6.85e-23 101
Msa1223560 MtrunA17_Chr7g0218081 52.459 61 27 2 257 317 228 286 8.41e-13 70.5
Msa1223560 MtrunA17_Chr7g0270191 46.316 95 46 2 241 335 129 218 8.26e-23 98.6
Msa1223560 MtrunA17_Chr7g0224801 52.113 71 33 1 246 316 82 151 2.47e-22 94.0
Msa1223560 MtrunA17_Chr5g0418191 50.617 81 39 1 239 319 302 381 5.77e-22 98.2
Msa1223560 MtrunA17_Chr5g0418191 37.615 109 64 3 257 365 151 255 8.92e-13 70.1
Msa1223560 MtrunA17_Chr7g0249311 51.250 80 38 1 240 319 375 453 1.02e-21 98.6
Msa1223560 MtrunA17_Chr7g0249311 48.333 60 29 2 258 317 180 237 5.38e-11 65.1
Msa1223560 MtrunA17_Chr7g0249301 51.250 80 38 1 240 319 275 353 1.40e-21 96.3
Msa1223560 MtrunA17_Chr7g0249301 46.154 65 33 2 253 317 58 120 4.49e-11 64.7
Msa1223560 MtrunA17_Chr4g0042831 46.316 95 44 2 225 319 252 339 1.91e-21 96.3
Msa1223560 MtrunA17_Chr1g0194481 51.948 77 36 1 241 317 142 217 2.40e-21 94.7
Msa1223560 MtrunA17_Chr8g0386241 45.570 79 42 1 242 320 88 165 7.14e-21 90.1
Msa1223560 MtrunA17_Chr4g0051881 44.554 101 49 2 244 338 165 264 1.10e-20 93.2
Msa1223560 MtrunA17_Chr4g0068541 50.649 77 37 1 241 317 192 267 2.35e-20 93.2
Msa1223560 MtrunA17_Chr7g0263531 41.489 94 54 1 224 317 328 420 4.95e-20 92.4
Msa1223560 MtrunA17_Chr7g0263531 50.000 60 28 2 258 317 190 247 1.04e-11 66.6
Msa1223560 MtrunA17_Chr5g0418781 47.561 82 42 1 233 314 100 180 5.24e-20 90.1
Msa1223560 MtrunA17_Chr8g0383251 45.946 74 39 1 244 317 95 167 1.18e-19 86.7
Msa1223560 MtrunA17_Chr7g0270051 52.703 74 32 1 257 330 53 123 2.46e-19 87.8
Msa1223560 MtrunA17_Chr4g0074001 50.633 79 36 2 241 317 343 420 2.62e-19 90.9
Msa1223560 MtrunA17_Chr5g0443131 50.000 74 36 1 244 317 144 216 1.14e-18 86.7
Msa1223560 MtrunA17_Chr7g0248721 47.500 80 40 2 236 315 120 197 1.55e-18 84.3
Msa1223560 MtrunA17_Chr3g0129361 48.000 75 38 1 240 314 127 200 1.57e-18 84.3
Msa1223560 MtrunA17_Chr1g0147881 48.649 74 38 0 241 314 246 319 1.95e-18 86.7
Msa1223560 MtrunA17_Chr8g0335811 43.902 82 45 1 233 314 130 210 2.44e-18 84.0
Msa1223560 MtrunA17_Chr3g0129321 47.297 74 38 1 244 317 105 177 4.17e-18 82.8
Msa1223560 MtrunA17_Chr5g0401821 48.684 76 39 0 241 316 183 258 6.56e-18 84.0
Msa1223560 MtrunA17_Chr4g0053281 48.101 79 41 0 241 319 250 328 7.43e-18 84.7
Msa1223560 MtrunA17_Chr8g0379461 52.113 71 29 1 257 327 76 141 1.42e-17 82.4
Msa1223560 MtrunA17_Chr2g0319961 39.623 106 50 2 226 317 35 140 2.50e-17 82.0
Msa1223560 MtrunA17_Chr8g0348311 52.113 71 34 0 257 327 155 225 2.91e-17 83.2
Msa1223560 MtrunA17_Chr8g0383351 47.368 76 38 2 240 314 142 216 4.30e-17 80.5
Msa1223560 MtrunA17_Chr7g0228941 47.887 71 37 0 244 314 39 109 4.45e-17 77.4
Msa1223560 MtrunA17_Chr1g0188941 41.667 84 48 1 234 317 28 110 4.77e-17 77.0
Msa1223560 MtrunA17_Chr1g0184691 52.459 61 29 0 258 318 294 354 7.01e-17 82.0
Msa1223560 MtrunA17_Chr4g0071521 47.297 74 39 0 241 314 229 302 7.99e-17 81.3
Msa1223560 MtrunA17_Chr2g0284321 51.613 62 30 0 257 318 35 96 8.12e-17 79.3
Msa1223560 MtrunA17_Chr2g0293481 48.571 70 36 0 258 327 268 337 1.36e-16 82.0
Msa1223560 MtrunA17_Chr3g0139021 45.946 74 40 0 241 314 298 371 5.63e-16 79.7
Msa1223560 MtrunA17_Chr2g0289181 34.074 135 79 3 252 376 9 143 6.99e-16 77.0
Msa1223560 MtrunA17_Chr7g0248691 37.607 117 71 2 213 327 108 224 8.90e-16 78.6
Msa1223560 MtrunA17_Chr6g0485431 48.000 75 37 2 244 317 141 214 1.22e-14 75.5
Msa1223560 MtrunA17_Chr7g0238921 38.318 107 50 2 230 321 82 187 1.30e-14 74.7
Msa1223560 MtrunA17_Chr8g0387501 44.286 70 39 0 258 327 130 199 1.31e-14 73.9
Msa1223560 MtrunA17_Chr1g0189391 43.056 72 39 2 244 314 84 154 1.03e-13 72.4
Msa1223560 MtrunA17_Chr1g0202461 43.421 76 43 0 258 333 262 337 1.86e-13 72.0
Msa1223560 MtrunA17_Chr3g0126391 53.333 60 26 1 256 313 132 191 5.47e-13 70.1
Msa1223560 MtrunA17_Chr5g0432071 38.462 78 46 2 257 334 104 179 1.33e-12 67.0
Msa1223560 MtrunA17_Chr8g0347251 47.826 69 34 1 256 322 135 203 1.92e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1223560 AT1G62300.1 50.787 508 186 17 4 466 50 538 4.41e-125 376
Msa1223560 AT4G04450.1 48.156 488 195 15 28 466 25 503 1.31e-124 374
Msa1223560 AT4G22070.4 47.860 514 169 15 27 466 26 514 4.98e-111 339
Msa1223560 AT4G22070.3 47.860 514 169 15 27 466 26 514 4.98e-111 339
Msa1223560 AT4G22070.2 47.860 514 169 15 27 466 26 514 4.98e-111 339
Msa1223560 AT4G22070.1 47.860 514 169 15 27 466 26 514 4.98e-111 339
Msa1223560 AT4G04450.2 49.761 418 158 13 92 466 1 409 5.87e-110 333
Msa1223560 AT4G01720.1 45.312 384 169 15 96 463 85 443 1.53e-66 222
Msa1223560 AT5G15130.2 44.118 272 115 5 229 468 157 423 8.09e-50 178
Msa1223560 AT5G15130.1 44.118 272 115 5 229 468 199 465 9.44e-50 179
Msa1223560 AT1G68150.1 42.194 237 110 6 113 338 96 316 4.63e-47 167
Msa1223560 AT1G18860.1 42.069 290 107 8 240 468 222 511 5.35e-44 162
Msa1223560 AT1G69810.1 36.364 308 155 10 107 380 22 322 5.39e-38 143
Msa1223560 AT1G69810.2 54.930 142 58 3 240 380 128 264 2.00e-36 137
Msa1223560 AT4G31800.1 35.270 241 125 5 104 335 36 254 3.22e-33 128
Msa1223560 AT1G80840.1 34.034 238 108 7 91 317 7 206 3.85e-31 122
Msa1223560 AT2G25000.1 32.870 216 103 4 106 321 37 210 1.05e-26 109
Msa1223560 AT2G25000.2 33.173 208 97 4 114 321 2 167 2.02e-26 107
Msa1223560 AT2G25000.4 33.173 208 97 4 114 321 2 167 2.02e-26 107
Msa1223560 AT2G25000.3 33.173 208 97 4 114 321 24 189 3.61e-26 107
Msa1223560 AT4G31800.2 55.000 80 36 0 256 335 110 189 3.25e-25 104
Msa1223560 AT2G38470.1 53.086 81 37 1 239 319 344 423 2.80e-24 106
Msa1223560 AT2G38470.1 46.575 73 36 3 245 317 173 242 8.49e-13 70.9
Msa1223560 AT4G26640.5 55.128 78 34 1 240 317 188 264 6.86e-24 103
Msa1223560 AT4G26640.5 38.095 84 50 2 234 317 12 93 4.28e-12 68.2
Msa1223560 AT4G26640.6 55.128 78 34 1 240 317 188 264 6.86e-24 103
Msa1223560 AT4G26640.6 38.095 84 50 2 234 317 12 93 4.28e-12 68.2
Msa1223560 AT4G26640.4 55.128 78 34 1 240 317 188 264 6.86e-24 103
Msa1223560 AT4G26640.4 38.095 84 50 2 234 317 12 93 4.28e-12 68.2
Msa1223560 AT4G26640.3 55.128 78 34 1 240 317 188 264 6.86e-24 103
Msa1223560 AT4G26640.3 38.095 84 50 2 234 317 12 93 4.28e-12 68.2
Msa1223560 AT4G26640.7 55.128 78 34 1 240 317 324 400 1.61e-23 103
Msa1223560 AT4G26640.7 38.095 84 50 2 234 317 148 229 6.59e-12 68.2
Msa1223560 AT4G26640.1 55.128 78 34 1 240 317 292 368 1.72e-23 103
Msa1223560 AT4G26640.1 38.095 84 50 2 234 317 116 197 6.50e-12 68.2
Msa1223560 AT4G26640.2 55.128 78 34 1 240 317 364 440 2.02e-23 103
Msa1223560 AT4G26640.2 38.095 84 50 2 234 317 188 269 6.81e-12 68.2
Msa1223560 AT5G56270.1 50.000 90 44 1 230 319 460 548 2.62e-23 103
Msa1223560 AT5G56270.1 49.180 61 29 2 258 318 274 332 1.94e-11 67.0
Msa1223560 AT5G26170.1 52.778 72 33 1 243 314 99 169 3.77e-23 96.7
Msa1223560 AT2G04880.2 47.674 86 44 1 236 321 262 346 4.37e-23 102
Msa1223560 AT2G04880.2 31.081 148 82 5 255 391 109 247 6.15e-13 71.2
Msa1223560 AT2G04880.1 47.674 86 44 1 236 321 286 370 5.59e-23 102
Msa1223560 AT2G04880.1 47.143 70 34 3 255 323 109 176 1.38e-12 70.1
Msa1223560 AT5G49520.1 51.948 77 36 1 241 317 205 280 6.45e-23 100
Msa1223560 AT1G29860.1 46.392 97 48 2 241 337 120 212 6.65e-23 99.0
Msa1223560 AT5G07100.2 48.276 87 44 1 231 317 115 200 8.50e-23 97.1
Msa1223560 AT5G07100.2 39.189 74 44 1 257 330 24 96 4.06e-11 63.2
Msa1223560 AT5G07100.4 48.276 87 44 1 231 317 115 200 8.50e-23 97.1
Msa1223560 AT5G07100.4 39.189 74 44 1 257 330 24 96 4.06e-11 63.2
Msa1223560 AT5G07100.5 48.276 87 44 1 231 317 115 200 8.50e-23 97.1
Msa1223560 AT5G07100.5 39.189 74 44 1 257 330 24 96 4.06e-11 63.2
Msa1223560 AT5G07100.3 48.276 87 44 1 231 317 115 200 8.50e-23 97.1
Msa1223560 AT5G07100.3 39.189 74 44 1 257 330 24 96 4.06e-11 63.2
Msa1223560 AT1G13960.1 51.190 84 40 1 234 317 386 468 1.19e-22 101
Msa1223560 AT1G13960.1 49.180 61 29 2 257 317 229 287 5.74e-12 68.2
Msa1223560 AT1G13960.2 51.190 84 40 1 234 317 233 315 1.20e-22 99.8
Msa1223560 AT1G13960.2 49.180 61 29 2 257 317 76 134 6.89e-12 67.4
Msa1223560 AT5G07100.1 48.276 87 44 1 231 317 208 293 1.28e-22 98.6
Msa1223560 AT5G07100.1 39.189 74 44 1 257 330 117 189 9.06e-11 63.5
Msa1223560 AT2G03340.1 48.837 86 43 1 234 319 392 476 1.11e-21 98.6
Msa1223560 AT2G03340.1 49.180 61 29 2 257 317 250 308 2.55e-12 69.3
Msa1223560 AT4G18170.1 51.948 77 36 1 241 317 156 231 1.57e-21 95.5
Msa1223560 AT5G13080.1 53.425 73 33 1 244 316 54 125 1.90e-21 90.9
Msa1223560 AT2G47260.1 45.161 93 50 1 233 325 150 241 2.02e-21 95.5
Msa1223560 AT1G64000.1 45.882 85 45 1 231 315 88 171 3.03e-21 92.0
Msa1223560 AT5G41570.1 51.250 80 37 2 237 315 77 155 6.05e-21 90.5
Msa1223560 AT2G46130.1 47.126 87 45 1 229 315 2 87 7.38e-21 88.2
Msa1223560 AT4G31800.3 32.500 200 104 5 104 294 36 213 1.06e-20 91.7
Msa1223560 AT4G26440.1 41.071 112 63 2 230 341 345 453 1.86e-20 95.1
Msa1223560 AT1G55600.1 43.333 90 50 1 230 319 280 368 2.23e-20 94.4
Msa1223560 AT3G62340.1 53.165 79 36 1 244 322 105 182 2.28e-20 91.7
Msa1223560 AT3G01080.1 46.429 84 44 1 234 317 283 365 2.34e-20 93.6
Msa1223560 AT3G01080.1 49.180 61 29 2 257 317 167 225 8.24e-11 64.3
Msa1223560 AT3G01080.2 49.367 79 39 1 239 317 326 403 2.61e-20 94.0
Msa1223560 AT3G01080.2 49.180 61 29 2 257 317 205 263 8.99e-11 64.3
Msa1223560 AT1G69310.4 45.783 83 44 1 232 314 122 203 4.22e-20 90.9
Msa1223560 AT1G69310.2 45.783 83 44 1 232 314 122 203 4.22e-20 90.9
Msa1223560 AT1G69310.1 45.783 83 44 1 232 314 122 203 4.22e-20 90.9
Msa1223560 AT2G30250.1 50.000 80 39 1 240 319 311 389 4.28e-20 92.4
Msa1223560 AT4G30935.1 41.176 119 58 3 224 334 298 412 4.32e-20 93.2
Msa1223560 AT5G46350.1 50.000 74 36 1 244 317 170 242 4.59e-20 91.7
Msa1223560 AT3G01970.1 51.389 72 34 1 243 314 51 121 1.42e-19 85.9
Msa1223560 AT2G23320.1 59.322 59 24 0 258 316 241 299 1.06e-18 87.4
Msa1223560 AT2G30590.1 50.000 82 40 1 241 321 297 378 1.15e-18 88.2
Msa1223560 AT3G58710.1 42.056 107 47 2 226 317 25 131 1.25e-18 86.3
Msa1223560 AT5G64810.1 47.887 71 36 1 244 314 97 166 1.26e-18 84.3
Msa1223560 AT3G58710.2 42.453 106 47 2 226 317 25 130 1.87e-18 85.9
Msa1223560 AT2G44745.1 46.667 75 39 1 240 314 128 201 3.73e-18 83.6
Msa1223560 AT5G28650.1 62.264 53 20 0 262 314 267 319 4.01e-18 85.9
Msa1223560 AT1G29280.1 52.459 61 29 0 257 317 74 134 7.23e-18 84.0
Msa1223560 AT4G39410.1 48.000 75 38 1 240 314 206 279 9.23e-18 84.3
Msa1223560 AT3G04670.1 57.895 57 24 0 258 314 263 319 2.31e-17 83.6
Msa1223560 AT4G24240.1 48.684 76 39 0 241 316 265 340 3.23e-17 83.6
Msa1223560 AT4G31550.2 48.649 74 38 0 241 314 229 302 3.47e-17 82.8
Msa1223560 AT4G31550.1 48.649 74 38 0 241 314 230 303 3.54e-17 82.8
Msa1223560 AT2G24570.1 48.649 74 38 0 241 314 227 300 5.00e-17 82.4
Msa1223560 AT4G01250.1 40.426 94 51 1 239 327 105 198 1.56e-16 80.5
Msa1223560 AT5G52830.1 46.753 77 41 0 251 327 159 235 1.79e-16 81.3
Msa1223560 AT2G37260.3 45.977 87 41 3 252 333 264 349 1.81e-16 81.3
Msa1223560 AT2G37260.3 43.243 74 40 2 258 331 86 157 7.51e-14 73.2
Msa1223560 AT2G37260.2 45.977 87 41 3 252 333 262 347 1.84e-16 81.3
Msa1223560 AT2G37260.2 43.243 74 40 2 258 331 84 155 7.60e-14 73.2
Msa1223560 AT2G34830.1 46.053 76 40 1 243 317 200 275 2.14e-16 81.6
Msa1223560 AT1G30650.1 46.053 76 40 1 243 317 202 277 2.35e-16 81.6
Msa1223560 AT2G34830.2 46.053 76 40 1 243 317 209 284 2.45e-16 81.6
Msa1223560 AT2G37260.1 45.977 87 41 3 252 333 344 429 3.88e-16 80.9
Msa1223560 AT2G37260.1 43.243 74 40 2 258 331 166 237 1.29e-13 73.2
Msa1223560 AT2G46130.2 43.678 87 44 2 229 315 2 83 7.26e-16 73.9
Msa1223560 AT4G23550.1 41.975 81 47 0 252 332 135 215 1.27e-15 78.2
Msa1223560 AT1G69310.3 42.169 83 43 2 232 314 122 199 3.47e-15 76.3
Msa1223560 AT5G43290.1 35.455 110 52 3 214 322 87 178 1.13e-14 74.7
Msa1223560 AT5G45050.2 40.449 89 41 1 257 333 1152 1240 1.94e-13 73.6
Msa1223560 AT5G45050.1 40.449 89 41 1 257 333 1180 1268 2.13e-13 73.6
Msa1223560 AT5G45050.4 40.449 89 41 1 257 333 1041 1129 2.33e-13 73.6
Msa1223560 AT2G21900.1 43.548 62 34 1 256 317 108 168 2.81e-13 69.3
Msa1223560 AT2G46400.1 42.500 80 44 1 236 313 83 162 4.13e-12 67.4
Msa1223560 AT5G24110.1 50.000 60 28 1 256 313 112 171 6.03e-12 67.0
Msa1223560 AT5G45260.1 37.255 102 57 3 233 332 1189 1285 8.49e-12 68.6
Msa1223560 AT2G40740.1 35.849 106 51 3 257 345 173 278 2.84e-11 64.7
Msa1223560 AT2G40740.3 35.849 106 51 3 257 345 182 287 3.57e-11 64.7
Msa1223560 AT3G01080.3 49.180 61 29 2 257 317 205 263 5.25e-11 64.3

Find 130 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGAAGGCCGGAGAATT+TGG 0.150973 8_1:+88068137 None:intergenic
AAGAATTCTGGCTAGTAAAT+TGG 0.190593 8_1:+88068347 None:intergenic
TCAGCATTTCCTTCAGCTTT+TGG 0.196513 8_1:+88069362 None:intergenic
GCTGCTACTGTGTTACTATC+TGG 0.288928 8_1:-88068402 Msa1223560:CDS
GGGATTACTGTTATTGTTGC+TGG 0.289882 8_1:+88067978 None:intergenic
GGCATTGGAGGGGTTTAATC+TGG 0.291166 8_1:+88068966 None:intergenic
GGGAGGAGCAGCACCAATTA+TGG 0.310343 8_1:+88067999 None:intergenic
GAGGCTGTCCAAGCTGGAAT+TGG 0.315291 8_1:+88068217 None:intergenic
CTGTTTGGCAAGAGAGCTTA+TGG 0.316403 8_1:-88067881 Msa1223560:three_prime_UTR
CACTTCCGGTGTTAGTAATA+AGG 0.320090 8_1:+88069696 None:intergenic
AGAATTCTGGCTAGTAAATT+GGG 0.323046 8_1:+88068348 None:intergenic
TTCTGAGGTGTGCATGGTTC+AGG 0.327374 8_1:+88069054 None:intergenic
ATCAGAACTAGTACAACTTT+TGG 0.338708 8_1:-88069004 Msa1223560:CDS
CACCATTGATTGATCACTTC+CGG 0.338711 8_1:+88069682 None:intergenic
GGATGCCAATGGCGAAAATA+TGG 0.339559 8_1:-88068757 Msa1223560:CDS
GAAATATCATCAAGTTGTTC+TGG 0.344687 8_1:+88070149 None:intergenic
GGTAGCACTGTTGATGATAT+TGG 0.345701 8_1:+88067957 None:intergenic
CCTGATCATGTTCTGTGGTT+TGG 0.349953 8_1:+88069272 None:intergenic
GCTGCTATCTCCTCCATAAT+TGG 0.361075 8_1:-88068012 Msa1223560:CDS
TGCTTGATCAGCGGCATTGG+AGG 0.372243 8_1:+88068954 None:intergenic
GTAGCACTGTTGATGATATT+GGG 0.374246 8_1:+88067958 None:intergenic
TTGGGTTCTATAAGTCTGTT+TGG 0.383456 8_1:-88067896 Msa1223560:three_prime_UTR
GCGAGTGCTGCCGTGAAGTT+TGG 0.385939 8_1:+88068036 None:intergenic
CGGCATGAATCGTGTAACTA+TGG 0.386196 8_1:-88070210 Msa1223560:CDS
CTTGGACAGCCTCAAAACTT+TGG 0.392430 8_1:-88068207 Msa1223560:CDS
GTTCTGTTGCTGTTGCATTA+AGG 0.395721 8_1:+88069294 None:intergenic
AGAGCAATTAGGAAGAATTC+TGG 0.399082 8_1:+88068335 None:intergenic
TTACAGATGCACAATGGCTC+TGG 0.402318 8_1:-88068695 Msa1223560:CDS
AGACTCACTTGTTTGCGAAC+GGG 0.403359 8_1:+88068667 None:intergenic
AGTTCTGATGCGTCTGGATC+AGG 0.404046 8_1:+88069018 None:intergenic
TGATCATCCACTTCTGCTGC+TGG 0.410282 8_1:+88069084 None:intergenic
CCTGTTGAGTTGGATGCAAC+TGG 0.412568 8_1:+88068091 None:intergenic
TACCATGTGGGCTTCTGATC+GGG 0.414425 8_1:+88068888 None:intergenic
TTCAGGAAGCTAGAAGCTCT+TGG 0.420968 8_1:-88067915 Msa1223560:three_prime_UTR
CAACATCTTGAGACATTTGA+AGG 0.433132 8_1:+88068124 None:intergenic
CTGTAATATGCGCGAGGACA+AGG 0.435386 8_1:+88068712 None:intergenic
TATCTGTAGTGTCGCGTGTA+AGG 0.437510 8_1:+88068259 None:intergenic
CCAGTTGCATCCAACTCAAC+AGG 0.445863 8_1:-88068091 Msa1223560:CDS
TCAGGAAGCTAGAAGCTCTT+GGG 0.449470 8_1:-88067914 Msa1223560:three_prime_UTR
AGGCTGTCCAAGCTGGAATT+GGG 0.454087 8_1:+88068218 None:intergenic
TCCTAATTGCTCTTCTAGCA+TGG 0.455237 8_1:-88068325 Msa1223560:CDS
TATAACCAATCCAAATTCTC+CGG 0.471393 8_1:-88068147 Msa1223560:CDS
GTGTGCATGGTTCAGGCTCT+TGG 0.473309 8_1:+88069061 None:intergenic
GAAATTGACCTGTTCAGTCT+TGG 0.478191 8_1:+88069622 None:intergenic
GCTGCGGTGGTCGATGCCAT+TGG 0.480782 8_1:+88068426 None:intergenic
CTGCTACTGTGTTACTATCT+GGG 0.480934 8_1:-88068401 Msa1223560:CDS
GCTTGATCAGCGGCATTGGA+GGG 0.485891 8_1:+88068955 None:intergenic
GAAGTTTGGATCCGCGGTGA+TGG 0.486096 8_1:+88068050 None:intergenic
CAAAACGCAAATAGACTCAT+AGG 0.486782 8_1:+88067858 None:intergenic
CAGCCTCAAAACTTTGGATC+AGG 0.489452 8_1:-88068201 Msa1223560:CDS
GGATTACTGTTATTGTTGCT+GGG 0.489961 8_1:+88067979 None:intergenic
TCTAGTGTCACAGTAGGAAA+TGG 0.493043 8_1:+88068282 None:intergenic
TGTACTAGTTCTGATGCGTC+TGG 0.496058 8_1:+88069012 None:intergenic
TAAGCGTGCCAAGACTGAAC+AGG 0.497408 8_1:-88069630 Msa1223560:intron
ATTTGCTTGATCAGCGGCAT+TGG 0.498345 8_1:+88068951 None:intergenic
GGCTCTTTCTGATTGTTCTG+AGG 0.499101 8_1:+88069039 None:intergenic
GGCATAGCAGCAGGTGGGAG+TGG 0.499597 8_1:+88068447 None:intergenic
ATCTTGAGACATTTGAAGGC+CGG 0.503538 8_1:+88068128 None:intergenic
TAGCAGCAGGTGGGAGTGGA+TGG 0.505934 8_1:+88068451 None:intergenic
GACTCACTTGTTTGCGAACG+GGG 0.507020 8_1:+88068668 None:intergenic
TCTCCTTCCCAATTCCAGCT+TGG 0.508244 8_1:-88068225 Msa1223560:CDS
TTTACATTTCAGATTGTCAA+AGG 0.510724 8_1:-88069159 Msa1223560:intron
CTTGATCAGCGGCATTGGAG+GGG 0.514641 8_1:+88068956 None:intergenic
CCAAACCACAGAACATGATC+AGG 0.515476 8_1:-88069272 Msa1223560:intron
ATACCATGTGGGCTTCTGAT+CGG 0.516534 8_1:+88068887 None:intergenic
ATGAATCTAATAAGCACAAA+CGG 0.517014 8_1:-88070281 Msa1223560:CDS
GGTCAATGTCAAGTGCTGAT+GGG 0.517767 8_1:-88068380 Msa1223560:CDS
GGGTCAATGTCAAGTGCTGA+TGG 0.517914 8_1:-88068381 Msa1223560:CDS
TGATTGTTCTGAGGTGTGCA+TGG 0.520062 8_1:+88069048 None:intergenic
GCCATGCTAGAAGAGCAATT+AGG 0.522733 8_1:+88068324 None:intergenic
AAAGGAAAAGCTGAGGAGAA+AGG 0.526527 8_1:-88069141 Msa1223560:CDS
GAGCAATTGCAAGAGGAGCT+TGG 0.529769 8_1:-88069403 Msa1223560:CDS
AAAGCTGAGGAGAAAGGAGA+TGG 0.531880 8_1:-88069135 Msa1223560:CDS
GCATTGGAGGGGTTTAATCT+GGG 0.534841 8_1:+88068967 None:intergenic
GATGCCATTGGCATAGCAGC+AGG 0.540165 8_1:+88068438 None:intergenic
GTTTGGATCCGCGGTGATGG+CGG 0.540992 8_1:+88068053 None:intergenic
GATCAATCAATGGTGGATGA+TGG 0.542601 8_1:-88069674 Msa1223560:CDS
TTAATCTGGGAAGCTGACTT+CGG 0.543926 8_1:+88068980 None:intergenic
TCACAATTGCAGATCAATGA+TGG 0.544968 8_1:-88068778 Msa1223560:intron
TGTGGGCTTCTGATCGGGCA+CGG 0.545553 8_1:+88068893 None:intergenic
TACAGATGCACAATGGCTCT+GGG 0.545795 8_1:-88068694 Msa1223560:CDS
AGTGATGATGCCTGTTGAGT+TGG 0.552709 8_1:+88068081 None:intergenic
CACCGGAAGTGATCAATCAA+TGG 0.556816 8_1:-88069684 Msa1223560:CDS
CTTCAAATGTCTCAAGATGT+TGG 0.558376 8_1:-88068123 Msa1223560:CDS
CAGACTCACTTGTTTGCGAA+CGG 0.559552 8_1:+88068666 None:intergenic
CACAGTAGCAGCAGCTGCGG+TGG 0.562058 8_1:+88068413 None:intergenic
GAATGGTCCAGCAGCAGAAG+TGG 0.565888 8_1:-88069091 Msa1223560:CDS
TTCTGCATTTGCTTGATCAG+CGG 0.571955 8_1:+88068945 None:intergenic
CCCACCTGCTGCTATGCCAA+TGG 0.574594 8_1:-88068442 Msa1223560:CDS
GAAGGCCGGAGAATTTGGAT+TGG 0.577352 8_1:+88068142 None:intergenic
CGCGACACTACAGATAATAA+CGG 0.582926 8_1:-88068252 Msa1223560:CDS
GCCATTGGCATAGCAGCAGG+TGG 0.589340 8_1:+88068441 None:intergenic
TGTAATATGCGCGAGGACAA+GGG 0.590385 8_1:+88068713 None:intergenic
CAACTCCTTATTACTAACAC+CGG 0.591469 8_1:-88069701 Msa1223560:CDS
CAAATGTCTCAAGATGTTGG+AGG 0.596501 8_1:-88068120 Msa1223560:CDS
AATCACCTGATCATGTTCTG+TGG 0.597094 8_1:+88069267 None:intergenic
GTGCTTATTAGATTCATCGT+CGG 0.598438 8_1:+88070287 None:intergenic
ATTATAGGTTCAACGTTGTG+CGG 0.604622 8_1:-88068517 Msa1223560:intron
CGCATATTACAGATGCACAA+TGG 0.609217 8_1:-88068701 Msa1223560:CDS
TGCAGAGAACCAAAAGCTGA+AGG 0.611336 8_1:-88069371 Msa1223560:CDS
CACCGCGGATCCAAACTTCA+CGG 0.616851 8_1:-88068046 Msa1223560:CDS
TGCCCGATCAGAAGCCCACA+TGG 0.617135 8_1:-88068890 Msa1223560:intron
CGGAAGTGATCAATCAATGG+TGG 0.617601 8_1:-88069681 Msa1223560:CDS
TGCCGTGAAGTTTGGATCCG+CGG 0.618501 8_1:+88068044 None:intergenic
GTCAATGTCAAGTGCTGATG+GGG 0.622615 8_1:-88068379 Msa1223560:CDS
TGTCCAAGCTGGAATTGGGA+AGG 0.625433 8_1:+88068222 None:intergenic
TTAGACGGAGCAATTGCAAG+AGG 0.625946 8_1:-88069410 Msa1223560:intron
GAATTCTGGCTAGTAAATTG+GGG 0.628387 8_1:+88068349 None:intergenic
GTGGCTAGAAAGTTGATGAA+TGG 0.628570 8_1:-88069108 Msa1223560:CDS
GTAAGGTCTAGTGTCACAGT+AGG 0.632116 8_1:+88068276 None:intergenic
TGAGGAGAAAGGAGATGGTG+TGG 0.634590 8_1:-88069130 Msa1223560:CDS
TTACTGTTATTGTTGCTGGG+AGG 0.638488 8_1:+88067982 None:intergenic
ATAGGTTCAACGTTGTGCGG+AGG 0.648017 8_1:-88068514 Msa1223560:intron
TGGTGCTGATGCTGAGAGTG+TGG 0.648731 8_1:+88068302 None:intergenic
CCATTGGCATAGCAGCAGGT+GGG 0.656238 8_1:+88068442 None:intergenic
GATTGTCAAAGGAAAAGCTG+AGG 0.658561 8_1:-88069148 Msa1223560:CDS
AGTGCTTGTGCAATCACTTG+TGG 0.658579 8_1:+88068171 None:intergenic
AGGAGCAGCACCAATTATGG+AGG 0.660834 8_1:+88068002 None:intergenic
TAACACAGTAGCAGCAGCTG+CGG 0.661912 8_1:+88068410 None:intergenic
TAAGCACAAACGGAGCAACA+TGG 0.662166 8_1:-88070271 Msa1223560:CDS
CTGTTGAGTTGGATGCAACT+GGG 0.664888 8_1:+88068092 None:intergenic
GTGCATCTGTAATATGCGCG+AGG 0.676244 8_1:+88068706 None:intergenic
ACTAATATAGTATACCATGT+GGG 0.681722 8_1:+88068876 None:intergenic
AGATCAATGATGGATGCCAA+TGG 0.705200 8_1:-88068768 Msa1223560:CDS
AATGAAGATGATCAAAATCG+CGG 0.707922 8_1:-88070233 Msa1223560:CDS
GGAGAAAGGAGATGGTGTGG+TGG 0.745088 8_1:-88069127 Msa1223560:CDS
TCAGCGCGGAGATTGCAATG+AGG 0.745989 8_1:+88070086 None:intergenic
ACTAAGTGCCGCCATCACCG+CGG 0.753006 8_1:-88068061 Msa1223560:CDS
GAAGATGATCAAAATCGCGG+CGG 0.753200 8_1:-88070230 Msa1223560:CDS
TTACGTTGACATGGTCAGCG+CGG 0.778275 8_1:+88070072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAGAAAGAAAAAA+TGG + chr8_1:88068095-88068114 None:intergenic 10.0%
!! ATTAGTGAAATAAAAAATAT+TGG - chr8_1:88068264-88068283 Msa1223560:CDS 10.0%
!! AATGTGAAAATATTTATCAT+AGG - chr8_1:88068360-88068379 Msa1223560:CDS 15.0%
!! ATGTGAAAATATTTATCATA+GGG - chr8_1:88068361-88068380 Msa1223560:CDS 15.0%
!! AAAATGAAATTAAACTAAGA+TGG + chr8_1:88068551-88068570 None:intergenic 15.0%
!!! TTAGTTTAATTTCATTTTAC+AGG - chr8_1:88068553-88068572 Msa1223560:intron 15.0%
!!! TAGTTTAATTTCATTTTACA+GGG - chr8_1:88068554-88068573 Msa1223560:intron 15.0%
!!! TGTTTCATTTGAAATATTAT+AGG - chr8_1:88069608-88069627 Msa1223560:intron 15.0%
!! AGTTACAAAGTAGAAATATA+AGG - chr8_1:88068125-88068144 Msa1223560:CDS 20.0%
!! AGAAATATAAGGAAAATACT+TGG - chr8_1:88068136-88068155 Msa1223560:CDS 20.0%
!! GAATAAAACAAACAGATATT+TGG + chr8_1:88068170-88068189 None:intergenic 20.0%
!! AATATTTATCATAGGGATTA+CGG - chr8_1:88068368-88068387 Msa1223560:CDS 20.0%
!!! AGTTTAATTTCATTTTACAG+GGG - chr8_1:88068555-88068574 Msa1223560:intron 20.0%
!!! ACACTTGTTAAATTTTTAGA+CGG - chr8_1:88068715-88068734 Msa1223560:CDS 20.0%
!!! GGTATAAAGATATTTTAAAC+TGG + chr8_1:88068915-88068934 None:intergenic 20.0%
!!! TCATAATTTCATTTCAAGTT+CGG + chr8_1:88068938-88068957 None:intergenic 20.0%
!! TTGAAATGAAATTATGAAAC+TGG - chr8_1:88068941-88068960 Msa1223560:CDS 20.0%
!! ATTAGTTAATTAAATCAGCT+AGG + chr8_1:88069339-88069358 None:intergenic 20.0%
!!! ATTTTGATAACAACATATGA+AGG - chr8_1:88069657-88069676 Msa1223560:CDS 20.0%
!!! TTTTGATAACAACATATGAA+GGG - chr8_1:88069658-88069677 Msa1223560:CDS 20.0%
! ATGAATCTAATAAGCACAAA+CGG - chr8_1:88067859-88067878 Msa1223560:three_prime_UTR 25.0%
!! AACAAACAGATATTTGGTTT+GGG + chr8_1:88068164-88068183 None:intergenic 25.0%
! AAACAAACAGATATTTGGTT+TGG + chr8_1:88068165-88068184 None:intergenic 25.0%
!!! ATAATTTCATTTCAAGTTCG+GGG + chr8_1:88068936-88068955 None:intergenic 25.0%
!!! CATAATTTCATTTCAAGTTC+GGG + chr8_1:88068937-88068956 None:intergenic 25.0%
! AATGAAATTATGAAACTGGA+TGG - chr8_1:88068945-88068964 Msa1223560:CDS 25.0%
! TTTACATTTCAGATTGTCAA+AGG - chr8_1:88068981-88069000 Msa1223560:CDS 25.0%
! ACTAATATAGTATACCATGT+GGG + chr8_1:88069267-88069286 None:intergenic 25.0%
!! AAAAATATGCTACTGATTGT+AGG + chr8_1:88070207-88070226 None:intergenic 25.0%
AATGAAGATGATCAAAATCG+CGG - chr8_1:88067907-88067926 Msa1223560:three_prime_UTR 30.0%
! GAAATATCATCAAGTTGTTC+TGG + chr8_1:88067994-88068013 None:intergenic 30.0%
!! ACAAACAGATATTTGGTTTG+GGG + chr8_1:88068163-88068182 None:intergenic 30.0%
!! TCATAATTACCTTTGAAGTG+TGG - chr8_1:88068294-88068313 Msa1223560:CDS 30.0%
TTGATGCAAATATTGTAGAC+TGG - chr8_1:88068412-88068431 Msa1223560:CDS 30.0%
! ATCAGAACTAGTACAACTTT+TGG - chr8_1:88069136-88069155 Msa1223560:CDS 30.0%
CACTAATATAGTATACCATG+TGG + chr8_1:88069268-88069287 None:intergenic 30.0%
GCGAAAATATGGACAAAAAA+TGG - chr8_1:88069394-88069413 Msa1223560:CDS 30.0%
TATGGACAAAAAATGGCTAA+AGG - chr8_1:88069401-88069420 Msa1223560:CDS 30.0%
! AGAATTCTGGCTAGTAAATT+GGG + chr8_1:88069795-88069814 None:intergenic 30.0%
! AAGAATTCTGGCTAGTAAAT+TGG + chr8_1:88069796-88069815 None:intergenic 30.0%
TATAACCAATCCAAATTCTC+CGG - chr8_1:88069993-88070012 Msa1223560:intron 30.0%
! CAATCAGTAGCATATTTTTC+AGG - chr8_1:88070208-88070227 Msa1223560:CDS 30.0%
GTGCTTATTAGATTCATCGT+CGG + chr8_1:88067856-88067875 None:intergenic 35.0%
AAAAATGGCTTACGTTGACA+TGG + chr8_1:88068080-88068099 None:intergenic 35.0%
AAATCACAACCACACTTCAA+AGG + chr8_1:88068306-88068325 None:intergenic 35.0%
CAACTCCTTATTACTAACAC+CGG - chr8_1:88068439-88068458 Msa1223560:CDS 35.0%
TCACAATTGCAGATCAATGA+TGG - chr8_1:88069362-88069381 Msa1223560:CDS 35.0%
! TTTGTCCATATTTTCGCCAT+TGG + chr8_1:88069391-88069410 None:intergenic 35.0%
ATTATAGGTTCAACGTTGTG+CGG - chr8_1:88069623-88069642 Msa1223560:intron 35.0%
! GAATTCTGGCTAGTAAATTG+GGG + chr8_1:88069794-88069813 None:intergenic 35.0%
AGAGCAATTAGGAAGAATTC+TGG + chr8_1:88069808-88069827 None:intergenic 35.0%
CAACATCTTGAGACATTTGA+AGG + chr8_1:88070019-88070038 None:intergenic 35.0%
CTTCAAATGTCTCAAGATGT+TGG - chr8_1:88070017-88070036 Msa1223560:intron 35.0%
GGATTACTGTTATTGTTGCT+GGG + chr8_1:88070164-88070183 None:intergenic 35.0%
!! GTAGCACTGTTGATGATATT+GGG + chr8_1:88070185-88070204 None:intergenic 35.0%
TTGGGTTCTATAAGTCTGTT+TGG - chr8_1:88070244-88070263 Msa1223560:CDS 35.0%
CAAAACGCAAATAGACTCAT+AGG + chr8_1:88070285-88070304 None:intergenic 35.0%
! CACTTCCGGTGTTAGTAATA+AGG + chr8_1:88068447-88068466 None:intergenic 40.0%
! CACCATTGATTGATCACTTC+CGG + chr8_1:88068461-88068480 None:intergenic 40.0%
!! GATCAATCAATGGTGGATGA+TGG - chr8_1:88068466-88068485 Msa1223560:CDS 40.0%
GAAATTGACCTGTTCAGTCT+TGG + chr8_1:88068521-88068540 None:intergenic 40.0%
! TCAGCATTTCCTTCAGCTTT+TGG + chr8_1:88068781-88068800 None:intergenic 40.0%
! GTTCTGTTGCTGTTGCATTA+AGG + chr8_1:88068849-88068868 None:intergenic 40.0%
AATCACCTGATCATGTTCTG+TGG + chr8_1:88068876-88068895 None:intergenic 40.0%
GATTGTCAAAGGAAAAGCTG+AGG - chr8_1:88068992-88069011 Msa1223560:CDS 40.0%
AAAGGAAAAGCTGAGGAGAA+AGG - chr8_1:88068999-88069018 Msa1223560:CDS 40.0%
!! GTGGCTAGAAAGTTGATGAA+TGG - chr8_1:88069032-88069051 Msa1223560:CDS 40.0%
! TTAATCTGGGAAGCTGACTT+CGG + chr8_1:88069163-88069182 None:intergenic 40.0%
TTCTGCATTTGCTTGATCAG+CGG + chr8_1:88069198-88069217 None:intergenic 40.0%
!! AGATCAATGATGGATGCCAA+TGG - chr8_1:88069372-88069391 Msa1223560:CDS 40.0%
CGCATATTACAGATGCACAA+TGG - chr8_1:88069439-88069458 Msa1223560:intron 40.0%
GACGCATATTATGAGACGAA+AGG + chr8_1:88069578-88069597 None:intergenic 40.0%
! CTGCTACTGTGTTACTATCT+GGG - chr8_1:88069739-88069758 Msa1223560:intron 40.0%
TCCTAATTGCTCTTCTAGCA+TGG - chr8_1:88069815-88069834 Msa1223560:intron 40.0%
TCTAGTGTCACAGTAGGAAA+TGG + chr8_1:88069861-88069880 None:intergenic 40.0%
CGCGACACTACAGATAATAA+CGG - chr8_1:88069888-88069907 Msa1223560:intron 40.0%
! TGTCCTGATCCAAAGTTTTG+AGG + chr8_1:88069945-88069964 None:intergenic 40.0%
ATCTTGAGACATTTGAAGGC+CGG + chr8_1:88070015-88070034 None:intergenic 40.0%
CAAATGTCTCAAGATGTTGG+AGG - chr8_1:88070020-88070039 Msa1223560:intron 40.0%
TTACTGTTATTGTTGCTGGG+AGG + chr8_1:88070161-88070180 None:intergenic 40.0%
GGGATTACTGTTATTGTTGC+TGG + chr8_1:88070165-88070184 None:intergenic 40.0%
!! GGTAGCACTGTTGATGATAT+TGG + chr8_1:88070186-88070205 None:intergenic 40.0%
TAAGCACAAACGGAGCAACA+TGG - chr8_1:88067869-88067888 Msa1223560:three_prime_UTR 45.0%
GAAGATGATCAAAATCGCGG+CGG - chr8_1:88067910-88067929 Msa1223560:three_prime_UTR 45.0%
CGGCATGAATCGTGTAACTA+TGG - chr8_1:88067930-88067949 Msa1223560:exon 45.0%
CACCGGAAGTGATCAATCAA+TGG - chr8_1:88068456-88068475 Msa1223560:CDS 45.0%
CGGAAGTGATCAATCAATGG+TGG - chr8_1:88068459-88068478 Msa1223560:CDS 45.0%
TTAGACGGAGCAATTGCAAG+AGG - chr8_1:88068730-88068749 Msa1223560:CDS 45.0%
TGCAGAGAACCAAAAGCTGA+AGG - chr8_1:88068769-88068788 Msa1223560:CDS 45.0%
CCTGATCATGTTCTGTGGTT+TGG + chr8_1:88068871-88068890 None:intergenic 45.0%
CCAAACCACAGAACATGATC+AGG - chr8_1:88068868-88068887 Msa1223560:intron 45.0%
AAAGCTGAGGAGAAAGGAGA+TGG - chr8_1:88069005-88069024 Msa1223560:CDS 45.0%
!! TGATTGTTCTGAGGTGTGCA+TGG + chr8_1:88069095-88069114 None:intergenic 45.0%
GGCTCTTTCTGATTGTTCTG+AGG + chr8_1:88069104-88069123 None:intergenic 45.0%
!! TGTACTAGTTCTGATGCGTC+TGG + chr8_1:88069131-88069150 None:intergenic 45.0%
GCATTGGAGGGGTTTAATCT+GGG + chr8_1:88069176-88069195 None:intergenic 45.0%
ATTTGCTTGATCAGCGGCAT+TGG + chr8_1:88069192-88069211 None:intergenic 45.0%
! GACTCGTGCTTTTCTCATAG+TGG + chr8_1:88069222-88069241 None:intergenic 45.0%
ATACCATGTGGGCTTCTGAT+CGG + chr8_1:88069256-88069275 None:intergenic 45.0%
GGATGCCAATGGCGAAAATA+TGG - chr8_1:88069383-88069402 Msa1223560:CDS 45.0%
TGTAATATGCGCGAGGACAA+GGG + chr8_1:88069430-88069449 None:intergenic 45.0%
TTACAGATGCACAATGGCTC+TGG - chr8_1:88069445-88069464 Msa1223560:intron 45.0%
TACAGATGCACAATGGCTCT+GGG - chr8_1:88069446-88069465 Msa1223560:intron 45.0%
AGACTCACTTGTTTGCGAAC+GGG + chr8_1:88069476-88069495 None:intergenic 45.0%
CAGACTCACTTGTTTGCGAA+CGG + chr8_1:88069477-88069496 None:intergenic 45.0%
! GCTGCTACTGTGTTACTATC+TGG - chr8_1:88069738-88069757 Msa1223560:intron 45.0%
!! GGTCAATGTCAAGTGCTGAT+GGG - chr8_1:88069760-88069779 Msa1223560:intron 45.0%
!! GTCAATGTCAAGTGCTGATG+GGG - chr8_1:88069761-88069780 Msa1223560:intron 45.0%
GCCATGCTAGAAGAGCAATT+AGG + chr8_1:88069819-88069838 None:intergenic 45.0%
GTAAGGTCTAGTGTCACAGT+AGG + chr8_1:88069867-88069886 None:intergenic 45.0%
TATCTGTAGTGTCGCGTGTA+AGG + chr8_1:88069884-88069903 None:intergenic 45.0%
CTTGGACAGCCTCAAAACTT+TGG - chr8_1:88069933-88069952 Msa1223560:intron 45.0%
CAGCCTCAAAACTTTGGATC+AGG - chr8_1:88069939-88069958 Msa1223560:intron 45.0%
AGTGCTTGTGCAATCACTTG+TGG + chr8_1:88069972-88069991 None:intergenic 45.0%
CATTTGAAGGCCGGAGAATT+TGG + chr8_1:88070006-88070025 None:intergenic 45.0%
!! CTGTTGAGTTGGATGCAACT+GGG + chr8_1:88070051-88070070 None:intergenic 45.0%
! AGTGATGATGCCTGTTGAGT+TGG + chr8_1:88070062-88070081 None:intergenic 45.0%
GCTGCTATCTCCTCCATAAT+TGG - chr8_1:88070128-88070147 Msa1223560:CDS 45.0%
TTCAGGAAGCTAGAAGCTCT+TGG - chr8_1:88070225-88070244 Msa1223560:CDS 45.0%
TCAGGAAGCTAGAAGCTCTT+GGG - chr8_1:88070226-88070245 Msa1223560:CDS 45.0%
CTGTTTGGCAAGAGAGCTTA+TGG - chr8_1:88070259-88070278 Msa1223560:CDS 45.0%
TTACGTTGACATGGTCAGCG+CGG + chr8_1:88068071-88068090 None:intergenic 50.0%
TAAGCGTGCCAAGACTGAAC+AGG - chr8_1:88068510-88068529 Msa1223560:CDS 50.0%
GAGCAATTGCAAGAGGAGCT+TGG - chr8_1:88068737-88068756 Msa1223560:CDS 50.0%
TGAGGAGAAAGGAGATGGTG+TGG - chr8_1:88069010-88069029 Msa1223560:CDS 50.0%
TGATCATCCACTTCTGCTGC+TGG + chr8_1:88069059-88069078 None:intergenic 50.0%
!! TTCTGAGGTGTGCATGGTTC+AGG + chr8_1:88069089-88069108 None:intergenic 50.0%
! AGTTCTGATGCGTCTGGATC+AGG + chr8_1:88069125-88069144 None:intergenic 50.0%
! GGCATTGGAGGGGTTTAATC+TGG + chr8_1:88069177-88069196 None:intergenic 50.0%
TACCATGTGGGCTTCTGATC+GGG + chr8_1:88069255-88069274 None:intergenic 50.0%
CTGTAATATGCGCGAGGACA+AGG + chr8_1:88069431-88069450 None:intergenic 50.0%
GTGCATCTGTAATATGCGCG+AGG + chr8_1:88069437-88069456 None:intergenic 50.0%
GACTCACTTGTTTGCGAACG+GGG + chr8_1:88069475-88069494 None:intergenic 50.0%
CGTTCGCAAACAAGTGAGTC+TGG - chr8_1:88069475-88069494 Msa1223560:intron 50.0%
ATAGGTTCAACGTTGTGCGG+AGG - chr8_1:88069626-88069645 Msa1223560:intron 50.0%
TAACACAGTAGCAGCAGCTG+CGG + chr8_1:88069733-88069752 None:intergenic 50.0%
!! GGGTCAATGTCAAGTGCTGA+TGG - chr8_1:88069759-88069778 Msa1223560:intron 50.0%
TCTCCTTCCCAATTCCAGCT+TGG - chr8_1:88069915-88069934 Msa1223560:intron 50.0%
TGTCCAAGCTGGAATTGGGA+AGG + chr8_1:88069921-88069940 None:intergenic 50.0%
AGGCTGTCCAAGCTGGAATT+GGG + chr8_1:88069925-88069944 None:intergenic 50.0%
!!! AGTTTTGAGGCTGTCCAAGC+TGG + chr8_1:88069932-88069951 None:intergenic 50.0%
GAAGGCCGGAGAATTTGGAT+TGG + chr8_1:88070001-88070020 None:intergenic 50.0%
!! CCTGTTGAGTTGGATGCAAC+TGG + chr8_1:88070052-88070071 None:intergenic 50.0%
CCAGTTGCATCCAACTCAAC+AGG - chr8_1:88070049-88070068 Msa1223560:intron 50.0%
AGGAGCAGCACCAATTATGG+AGG + chr8_1:88070141-88070160 None:intergenic 50.0%
TCAGCGCGGAGATTGCAATG+AGG + chr8_1:88068057-88068076 None:intergenic 55.0%
GGAGAAAGGAGATGGTGTGG+TGG - chr8_1:88069013-88069032 Msa1223560:CDS 55.0%
GAATGGTCCAGCAGCAGAAG+TGG - chr8_1:88069049-88069068 Msa1223560:CDS 55.0%
GTGTGCATGGTTCAGGCTCT+TGG + chr8_1:88069082-88069101 None:intergenic 55.0%
! CTTGATCAGCGGCATTGGAG+GGG + chr8_1:88069187-88069206 None:intergenic 55.0%
! GCTTGATCAGCGGCATTGGA+GGG + chr8_1:88069188-88069207 None:intergenic 55.0%
! TGCTTGATCAGCGGCATTGG+AGG + chr8_1:88069189-88069208 None:intergenic 55.0%
CCATTGGCATAGCAGCAGGT+GGG + chr8_1:88069701-88069720 None:intergenic 55.0%
GATGCCATTGGCATAGCAGC+AGG + chr8_1:88069705-88069724 None:intergenic 55.0%
!! TGGTGCTGATGCTGAGAGTG+TGG + chr8_1:88069841-88069860 None:intergenic 55.0%
GAGGCTGTCCAAGCTGGAAT+TGG + chr8_1:88069926-88069945 None:intergenic 55.0%
GAAGTTTGGATCCGCGGTGA+TGG + chr8_1:88070093-88070112 None:intergenic 55.0%
CACCGCGGATCCAAACTTCA+CGG - chr8_1:88070094-88070113 Msa1223560:CDS 55.0%
TGCCGTGAAGTTTGGATCCG+CGG + chr8_1:88070099-88070118 None:intergenic 55.0%
GGGAGGAGCAGCACCAATTA+TGG + chr8_1:88070144-88070163 None:intergenic 55.0%
TGTGGGCTTCTGATCGGGCA+CGG + chr8_1:88069250-88069269 None:intergenic 60.0%
TGCCCGATCAGAAGCCCACA+TGG - chr8_1:88069250-88069269 Msa1223560:intron 60.0%
TAGCAGCAGGTGGGAGTGGA+TGG + chr8_1:88069692-88069711 None:intergenic 60.0%
CCCACCTGCTGCTATGCCAA+TGG - chr8_1:88069698-88069717 Msa1223560:CDS 60.0%
GCCATTGGCATAGCAGCAGG+TGG + chr8_1:88069702-88069721 None:intergenic 60.0%
! ACTAAGTGCCGCCATCACCG+CGG - chr8_1:88070079-88070098 Msa1223560:CDS 60.0%
! GTTTGGATCCGCGGTGATGG+CGG + chr8_1:88070090-88070109 None:intergenic 60.0%
! GCGAGTGCTGCCGTGAAGTT+TGG + chr8_1:88070107-88070126 None:intergenic 60.0%
GGCATAGCAGCAGGTGGGAG+TGG + chr8_1:88069696-88069715 None:intergenic 65.0%
GCTGCGGTGGTCGATGCCAT+TGG + chr8_1:88069717-88069736 None:intergenic 65.0%
CACAGTAGCAGCAGCTGCGG+TGG + chr8_1:88069730-88069749 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 88067848 88070314 88067848 ID=Msa1223560;Name=Msa1223560
chr8_1 mRNA 88067848 88070314 88067848 ID=Msa1223560-mRNA-1;Parent=Msa1223560;Name=Msa1223560-mRNA-1;_AED=0.05;_eAED=0.05;_QI=11|1|0.83|1|1|1|6|97|488
chr8_1 exon 88067848 88068532 88067848 ID=Msa1223560-mRNA-1:exon:31947;Parent=Msa1223560-mRNA-1
chr8_1 exon 88068675 88068788 88068675 ID=Msa1223560-mRNA-1:exon:31946;Parent=Msa1223560-mRNA-1
chr8_1 exon 88068891 88069169 88068891 ID=Msa1223560-mRNA-1:exon:31945;Parent=Msa1223560-mRNA-1
chr8_1 exon 88069273 88069428 88069273 ID=Msa1223560-mRNA-1:exon:31944;Parent=Msa1223560-mRNA-1
chr8_1 exon 88069631 88069732 88069631 ID=Msa1223560-mRNA-1:exon:31943;Parent=Msa1223560-mRNA-1
chr8_1 exon 88070076 88070314 88070076 ID=Msa1223560-mRNA-1:exon:31942;Parent=Msa1223560-mRNA-1
chr8_1 five_prime_UTR 88070304 88070314 88070304 ID=Msa1223560-mRNA-1:five_prime_utr;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88070076 88070303 88070076 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88069631 88069732 88069631 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88069273 88069428 88069273 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88068891 88069169 88068891 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88068675 88068788 88068675 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 CDS 88067945 88068532 88067945 ID=Msa1223560-mRNA-1:cds;Parent=Msa1223560-mRNA-1
chr8_1 three_prime_UTR 88067848 88067944 88067848 ID=Msa1223560-mRNA-1:three_prime_utr;Parent=Msa1223560-mRNA-1
Gene Sequence

>Msa1223560

ATGAATCTAATAAGCACAAACGGAGCAACATGGTGTGATGAGAATGAAAATGAAGATGATCAAAATCGCGGCGGCATGAATCGTGTAACTATGGATGAAGTTGATTTCTTCTCAAACGATAAATCATCATCGCCAGAACAACTTGATGATATTTCAATGAAGAATAATAATAATAATATTAATGAGATTTACGATCCTCATTGCAATCTCCGCGCTGACCATGTCAACACTGGTTTACAACTCCTTATTACTAACACCGGAAGTGATCAATCAATGGTGGATGATGGAACTTCAATAAATGCTGAAGATAAGCGTGCCAAGACTGAACAGACGGAGCAATTGCAAGAGGAGCTTGGACGTGTGAATGCAGAGAACCAAAAGCTGAAGGAAATGCTGAGTGACATGAGCAGTAGCTACACAAACTTGCATAAGCGTTTTATGTCCTTAATGCAACAGCAACAGAACCAAACCACAGAACATGATCAGATTGTCAAAGGAAAAGCTGAGGAGAAAGGAGATGGTGTGGTGGCTAGAAAGTTGATGAATGGTCCAGCAGCAGAAGTGGATGATCACCAAGAGCCTGAACCATGCACACCTCAGAACAATCAGAAAGAGCCTGATCCAGACGCATCAGAACTAGTACAACTTTTGGACCGAAGTCAGCTTCCCAGATTAAACCCCTCCAATGCCGCTGATCAAGCAAATGCAGAAGCCACTATGAGAAAAGCACGAGTCTCAGTCCGTGCCCGATCAGAAGCCCACATGATCAATGATGGATGCCAATGGCGAAAATATGGACAAAAAATGGCTAAAGGAAACCCTTGTCCTCGCGCATATTACAGATGCACAATGGCTCTGGGTTGCCCCGTTCGCAAACAAGTTCAACGTTGTGCGGAGGACAGATCAATTTTGATAACAACATATGAAGGGACACATAGCCATCCACTCCCACCTGCTGCTATGCCAATGGCATCGACCACCGCAGCTGCTGCTACTGTGTTACTATCTGGGTCAATGTCAAGTGCTGATGGGGTGATGAACCCCAATTTACTAGCCAGAATTCTTCCTAATTGCTCTTCTAGCATGGCCACACTCTCAGCATCAGCACCATTTCCTACTGTGACACTAGACCTTACACGCGACACTACAGATAATAACGGAAATTCTCCTTCCCAATTCCAGCTTGGACAGCCTCAAAACTTTGGATCAGGACAATTACCACAAGTGATTGCACAAGCACTTTATAACCAATCCAAATTCTCCGGCCTTCAAATGTCTCAAGATGTTGGAGGCTCTTCCCAGTTGCATCCAACTCAACAGGCATCATCACTAAGTGCCGCCATCACCGCGGATCCAAACTTCACGGCAGCACTCGCAGCTGCTATCTCCTCCATAATTGGTGCTGCTCCTCCCAGCAACAATAACAGTAATCCCAATATCATCAACAGTGCTACCTACAATCAGTAG
Protein sequence

>Msa1223560

MNLISTNGATWCDENENEDDQNRGGMNRVTMDEVDFFSNDKSSSPEQLDDISMKNNNNNINEIYDPHCNLRADHVNTGLQLLITNTGSDQSMVDDGTSINAEDKRAKTEQTEQLQEELGRVNAENQKLKEMLSDMSSSYTNLHKRFMSLMQQQQNQTTEHDQIVKGKAEEKGDGVVARKLMNGPAAEVDDHQEPEPCTPQNNQKEPDPDASELVQLLDRSQLPRLNPSNAADQANAEATMRKARVSVRARSEAHMINDGCQWRKYGQKMAKGNPCPRAYYRCTMALGCPVRKQVQRCAEDRSILITTYEGTHSHPLPPAAMPMASTTAAAATVLLSGSMSSADGVMNPNLLARILPNCSSSMATLSASAPFPTVTLDLTRDTTDNNGNSPSQFQLGQPQNFGSGQLPQVIAQALYNQSKFSGLQMSQDVGGSSQLHPTQQASSLSAAITADPNFTAALAAAISSIIGAAPPSNNNSNPNIINSATYNQ*