AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0324840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0324840 MtrunA17_Chr2g0302391 95.128 431 18 2 1 430 1 429 0.0 804
Msa0324840 MtrunA17_Chr3g0099641 47.162 229 108 6 187 411 225 444 5.20e-57 194
Msa0324840 MtrunA17_Chr7g0270831 33.973 365 185 10 64 417 92 411 1.51e-51 179
Msa0324840 MtrunA17_Chr4g0075201 36.786 280 160 4 68 340 120 389 4.93e-49 174
Msa0324840 MtrunA17_Chr2g0322391 35.854 410 176 16 66 410 167 554 1.12e-48 175
Msa0324840 MtrunA17_Chr2g0288871 32.581 399 206 10 68 413 143 531 3.24e-45 164
Msa0324840 MtrunA17_Chr4g0025061 50.000 164 73 2 198 359 345 501 3.24e-41 154
Msa0324840 MtrunA17_Chr4g0025051 54.430 158 65 3 184 340 306 457 3.37e-40 151
Msa0324840 MtrunA17_Chr4g0051181 50.658 152 64 4 197 340 227 375 2.82e-37 142
Msa0324840 MtrunA17_Chr4g0060271 63.158 95 31 1 183 273 221 315 9.84e-37 142
Msa0324840 MtrunA17_Chr7g0216891 61.386 101 38 1 187 286 192 292 2.63e-36 140
Msa0324840 MtrunA17_Chr4g0024391 39.241 237 120 7 192 410 208 438 1.66e-33 131
Msa0324840 MtrunA17_Chr4g0001361 42.857 105 58 2 194 296 141 245 5.25e-24 101
Msa0324840 MtrunA17_Chr5g0432161 49.020 102 50 2 174 275 383 482 1.78e-23 103
Msa0324840 MtrunA17_Chr5g0432161 46.667 60 30 2 214 273 245 302 7.70e-11 63.9
Msa0324840 MtrunA17_Chr2g0333851 39.850 133 70 3 196 323 147 274 1.08e-22 97.8
Msa0324840 MtrunA17_Chr7g0270191 37.671 146 80 4 197 332 129 273 1.55e-22 97.1
Msa0324840 MtrunA17_Chr1g0150271 48.235 85 42 2 211 293 99 183 2.63e-22 95.5
Msa0324840 MtrunA17_Chr3g0136961 50.602 83 38 1 211 293 136 215 3.19e-22 96.3
Msa0324840 MtrunA17_Chr1g0150281 50.685 73 36 0 198 270 125 197 5.32e-22 95.5
Msa0324840 MtrunA17_Chr3g0079691 37.584 149 81 5 163 301 435 581 3.12e-21 96.7
Msa0324840 MtrunA17_Chr3g0079691 39.362 94 54 3 185 277 251 342 9.99e-14 73.2
Msa0324840 MtrunA17_Chr5g0445311 50.633 79 38 1 197 275 348 425 3.97e-21 95.9
Msa0324840 MtrunA17_Chr7g0263531 44.954 109 52 3 186 289 329 434 8.04e-21 94.4
Msa0324840 MtrunA17_Chr7g0263531 42.308 78 43 2 214 291 190 265 3.01e-12 68.2
Msa0324840 MtrunA17_Chr3g0106491 51.282 78 37 1 196 273 348 424 9.01e-21 94.7
Msa0324840 MtrunA17_Chr5g0419241 65.517 58 19 1 185 241 48 105 1.36e-20 86.7
Msa0324840 MtrunA17_Chr3g0101131 50.633 79 38 1 197 275 399 476 1.73e-20 94.0
Msa0324840 MtrunA17_Chr4g0004911 50.000 82 37 2 195 273 315 395 3.12e-20 92.8
Msa0324840 MtrunA17_Chr4g0004911 37.864 103 60 3 179 281 153 251 2.91e-12 68.2
Msa0324840 MtrunA17_Chr7g0218081 47.674 86 44 1 200 285 397 481 6.05e-20 92.0
Msa0324840 MtrunA17_Chr7g0218081 48.333 60 29 2 214 273 229 286 1.30e-11 66.2
Msa0324840 MtrunA17_Chr4g0042831 45.977 87 46 1 198 284 263 348 7.08e-20 90.9
Msa0324840 MtrunA17_Chr7g0249311 48.148 81 41 1 195 275 374 453 8.04e-20 92.0
Msa0324840 MtrunA17_Chr7g0249301 48.148 81 41 1 195 275 274 353 1.20e-19 90.1
Msa0324840 MtrunA17_Chr7g0249301 43.939 66 35 2 212 277 61 124 6.82e-11 63.5
Msa0324840 MtrunA17_Chr5g0418191 45.161 93 47 2 200 289 307 398 1.65e-19 90.1
Msa0324840 MtrunA17_Chr5g0418191 50.000 60 28 2 214 273 152 209 5.40e-12 67.4
Msa0324840 MtrunA17_Chr3g0129321 51.351 74 35 1 200 273 105 177 1.76e-19 86.3
Msa0324840 MtrunA17_Chr4g0068541 47.674 86 43 2 197 282 192 275 2.06e-19 89.7
Msa0324840 MtrunA17_Chr2g0294231 45.122 82 44 1 197 278 187 267 5.38e-19 88.2
Msa0324840 MtrunA17_Chr2g0289181 49.296 71 36 0 214 284 15 85 5.70e-19 85.5
Msa0324840 MtrunA17_Chr7g0224801 45.263 95 47 2 180 270 56 149 6.69e-19 84.0
Msa0324840 MtrunA17_Chr5g0447701 40.708 113 61 3 184 292 223 333 6.78e-19 88.2
Msa0324840 MtrunA17_Chr5g0443131 34.711 121 75 2 160 277 101 220 1.24e-18 86.3
Msa0324840 MtrunA17_Chr1g0194481 44.444 81 44 1 197 277 142 221 1.29e-18 86.3
Msa0324840 MtrunA17_Chr7g0248721 34.503 171 85 7 118 283 58 206 1.79e-18 84.0
Msa0324840 MtrunA17_Chr4g0051881 35.252 139 70 4 200 325 165 296 1.93e-18 85.9
Msa0324840 MtrunA17_Chr7g0243891 47.500 80 41 1 196 275 357 435 2.21e-18 87.4
Msa0324840 MtrunA17_Chr8g0383251 47.297 74 38 1 200 273 95 167 2.43e-18 82.4
Msa0324840 MtrunA17_Chr2g0284321 55.738 61 27 0 214 274 36 96 4.87e-18 82.4
Msa0324840 MtrunA17_Chr1g0150801 49.315 73 36 1 198 270 87 158 5.12e-18 80.9
Msa0324840 MtrunA17_Chr4g0053281 54.839 62 28 0 214 275 267 328 8.29e-18 84.3
Msa0324840 MtrunA17_Chr8g0379461 56.667 60 26 0 214 273 77 136 3.82e-17 80.5
Msa0324840 MtrunA17_Chr5g0401821 41.837 98 48 1 184 272 161 258 4.90e-17 80.9
Msa0324840 MtrunA17_Chr4g0074001 44.156 77 42 1 197 273 343 418 7.78e-17 82.4
Msa0324840 MtrunA17_Chr5g0418781 42.683 82 46 1 190 271 101 181 8.31e-17 80.5
Msa0324840 MtrunA17_Chr8g0386241 34.646 127 73 4 159 282 52 171 8.82e-17 78.2
Msa0324840 MtrunA17_Chr7g0270051 50.725 69 34 0 214 282 54 122 9.41e-17 80.1
Msa0324840 MtrunA17_Chr1g0184691 52.459 61 29 0 214 274 294 354 1.13e-16 81.3
Msa0324840 MtrunA17_Chr1g0188941 43.373 83 46 1 191 273 29 110 2.19e-16 75.1
Msa0324840 MtrunA17_Chr1g0147881 52.542 59 28 0 214 272 263 321 5.09e-16 79.0
Msa0324840 MtrunA17_Chr2g0319961 55.000 60 27 0 214 273 81 140 6.06e-16 77.4
Msa0324840 MtrunA17_Chr7g0228941 50.000 58 29 0 214 271 53 110 6.97e-16 73.9
Msa0324840 MtrunA17_Chr8g0335811 45.882 85 41 2 190 270 127 210 8.75e-16 76.3
Msa0324840 MtrunA17_Chr2g0293481 39.394 99 59 1 188 285 241 339 1.22e-15 79.0
Msa0324840 MtrunA17_Chr3g0129361 41.053 95 53 2 197 291 128 219 1.40e-15 75.5
Msa0324840 MtrunA17_Chr8g0348311 51.429 70 34 0 214 283 156 225 2.14e-15 77.0
Msa0324840 MtrunA17_Chr1g0202461 43.678 87 49 0 213 299 261 347 6.10e-15 76.3
Msa0324840 MtrunA17_Chr8g0387501 42.353 85 47 1 212 296 128 210 7.05e-15 74.7
Msa0324840 MtrunA17_Chr7g0248691 50.000 70 35 0 214 283 155 224 1.28e-14 74.7
Msa0324840 MtrunA17_Chr4g0071521 52.632 57 27 0 214 270 246 302 1.84e-14 73.9
Msa0324840 MtrunA17_Chr8g0383351 48.438 64 32 1 207 270 154 216 8.40e-14 70.9
Msa0324840 MtrunA17_Chr3g0139021 39.744 78 47 0 197 274 298 375 1.12e-13 72.4
Msa0324840 MtrunA17_Chr1g0189391 31.915 141 92 3 131 270 17 154 1.63e-13 71.2
Msa0324840 MtrunA17_Chr6g0485431 52.308 65 29 2 214 277 155 218 2.99e-13 70.9
Msa0324840 MtrunA17_Chr7g0238921 52.632 57 26 1 214 270 125 180 7.17e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0324840 AT4G04450.2 37.942 311 145 7 70 340 20 322 6.36e-53 183
Msa0324840 AT4G04450.1 37.942 311 145 7 70 340 114 416 5.63e-52 182
Msa0324840 AT1G62300.1 36.386 404 172 17 74 413 158 540 2.30e-49 176
Msa0324840 AT4G01720.1 34.301 379 182 11 66 408 96 443 2.30e-46 167
Msa0324840 AT4G22070.4 34.969 326 154 9 66 343 108 423 2.10e-43 160
Msa0324840 AT4G22070.3 34.969 326 154 9 66 343 108 423 2.10e-43 160
Msa0324840 AT4G22070.2 34.969 326 154 9 66 343 108 423 2.10e-43 160
Msa0324840 AT4G22070.1 34.969 326 154 9 66 343 108 423 2.10e-43 160
Msa0324840 AT5G15130.1 34.524 336 143 10 68 342 35 354 9.61e-42 155
Msa0324840 AT5G15130.2 51.534 163 63 4 189 342 157 312 1.85e-41 154
Msa0324840 AT1G68150.1 64.815 108 37 1 191 298 213 319 6.11e-41 149
Msa0324840 AT1G18860.1 33.618 351 158 11 68 345 41 389 7.89e-37 141
Msa0324840 AT1G69810.2 44.172 163 78 3 182 342 114 265 1.57e-32 125
Msa0324840 AT1G69810.1 45.513 156 72 3 189 342 179 323 1.61e-32 127
Msa0324840 AT5G07100.2 39.130 138 73 4 148 282 81 210 3.48e-25 102
Msa0324840 AT5G07100.4 39.130 138 73 4 148 282 81 210 3.48e-25 102
Msa0324840 AT5G07100.5 39.130 138 73 4 148 282 81 210 3.48e-25 102
Msa0324840 AT5G07100.3 39.130 138 73 4 148 282 81 210 3.48e-25 102
Msa0324840 AT5G07100.1 38.571 140 75 4 146 282 172 303 4.97e-25 104
Msa0324840 AT1G80840.1 31.343 201 106 6 75 273 36 206 4.55e-22 96.3
Msa0324840 AT4G31800.2 56.000 75 32 1 207 280 104 178 6.36e-22 94.7
Msa0324840 AT4G31800.1 56.000 75 32 1 207 280 169 243 6.77e-22 95.9
Msa0324840 AT2G38470.1 42.857 119 64 3 158 275 308 423 7.56e-22 98.2
Msa0324840 AT2G38470.1 48.333 60 29 2 214 273 185 242 5.50e-11 64.7
Msa0324840 AT2G47260.1 48.810 84 42 1 197 280 158 240 1.32e-21 95.5
Msa0324840 AT1G29860.1 48.148 81 41 1 197 277 120 199 2.31e-21 94.0
Msa0324840 AT5G41570.1 48.352 91 42 2 184 270 65 154 2.42e-21 91.3
Msa0324840 AT2G30250.1 47.826 92 45 2 188 277 301 391 3.72e-21 95.1
Msa0324840 AT2G25000.2 55.556 72 31 1 206 276 95 166 5.59e-21 91.7
Msa0324840 AT2G25000.4 55.556 72 31 1 206 276 95 166 5.59e-21 91.7
Msa0324840 AT2G04880.2 49.351 77 38 1 199 275 269 344 5.88e-21 95.1
Msa0324840 AT2G04880.1 49.351 77 38 1 199 275 293 368 8.02e-21 95.1
Msa0324840 AT2G25000.3 55.556 72 31 1 206 276 117 188 1.01e-20 91.3
Msa0324840 AT4G18170.1 45.714 105 51 4 171 273 131 231 1.19e-20 92.4
Msa0324840 AT2G25000.1 55.556 72 31 1 206 276 138 209 1.27e-20 91.7
Msa0324840 AT1G64000.1 51.190 84 37 2 200 283 101 180 3.27e-20 88.6
Msa0324840 AT5G56270.1 48.864 88 44 1 188 275 462 548 5.24e-20 93.2
Msa0324840 AT4G26640.5 49.351 77 38 1 197 273 189 264 5.37e-20 91.7
Msa0324840 AT4G26640.6 49.351 77 38 1 197 273 189 264 5.37e-20 91.7
Msa0324840 AT4G26640.4 49.351 77 38 1 197 273 189 264 5.37e-20 91.7
Msa0324840 AT4G26640.3 49.351 77 38 1 197 273 189 264 5.37e-20 91.7
Msa0324840 AT4G26640.7 48.718 78 39 1 196 273 324 400 9.36e-20 92.0
Msa0324840 AT4G26640.1 49.351 77 38 1 197 273 293 368 1.05e-19 91.7
Msa0324840 AT4G26640.2 49.351 77 38 1 197 273 365 440 1.19e-19 91.7
Msa0324840 AT5G26170.1 52.778 72 33 1 199 270 99 169 1.46e-19 86.3
Msa0324840 AT1G13960.2 50.000 74 36 1 200 273 243 315 1.97e-19 89.7
Msa0324840 AT1G13960.2 50.000 60 28 2 214 273 77 134 1.28e-11 66.2
Msa0324840 AT1G13960.1 50.000 74 36 1 200 273 396 468 2.90e-19 90.5
Msa0324840 AT1G13960.1 50.000 60 28 2 214 273 230 287 9.97e-12 67.0
Msa0324840 AT3G62340.1 44.037 109 55 2 200 303 105 212 3.90e-19 87.4
Msa0324840 AT5G64810.1 48.276 87 41 2 185 271 85 167 4.58e-19 85.5
Msa0324840 AT5G13080.1 48.193 83 39 2 200 282 54 132 4.87e-19 84.0
Msa0324840 AT2G46130.1 45.263 95 48 2 189 283 6 96 4.91e-19 82.8
Msa0324840 AT2G03340.1 48.684 76 38 1 200 275 402 476 5.13e-19 89.7
Msa0324840 AT2G03340.1 51.667 60 27 2 214 273 251 308 9.38e-13 70.5
Msa0324840 AT5G49520.1 45.161 93 49 2 197 288 205 296 5.29e-19 89.0
Msa0324840 AT5G46350.1 41.667 96 54 2 200 295 170 263 5.45e-19 87.8
Msa0324840 AT3G01970.1 50.000 74 36 1 199 272 51 123 1.40e-18 82.4
Msa0324840 AT3G01080.1 48.649 74 37 1 200 273 293 365 1.65e-18 87.4
Msa0324840 AT3G01080.2 48.649 74 37 1 200 273 331 403 1.93e-18 87.8
Msa0324840 AT1G69310.4 45.238 84 45 1 188 271 122 204 2.17e-18 85.5
Msa0324840 AT1G69310.2 45.238 84 45 1 188 271 122 204 2.17e-18 85.5
Msa0324840 AT1G69310.1 45.238 84 45 1 188 271 122 204 2.17e-18 85.5
Msa0324840 AT4G26440.1 34.459 148 83 5 163 297 311 457 3.18e-18 87.4
Msa0324840 AT4G30935.1 37.302 126 63 3 184 295 303 426 7.42e-18 85.9
Msa0324840 AT2G37260.3 39.695 131 59 6 159 276 211 334 1.57e-17 84.0
Msa0324840 AT2G37260.3 44.156 77 39 3 213 289 85 157 3.27e-13 70.9
Msa0324840 AT2G37260.2 39.695 131 59 6 159 276 209 332 1.76e-17 84.0
Msa0324840 AT2G37260.2 44.156 77 39 3 213 289 83 155 3.54e-13 70.9
Msa0324840 AT2G37260.1 41.221 131 57 7 159 276 291 414 2.77e-17 84.0
Msa0324840 AT2G37260.1 44.156 77 39 3 213 289 165 237 4.55e-13 71.2
Msa0324840 AT2G23320.1 57.627 59 25 0 214 272 241 299 3.08e-17 82.8
Msa0324840 AT2G30590.1 54.688 64 28 1 214 276 314 377 7.79e-17 82.0
Msa0324840 AT5G28650.1 57.895 57 24 0 214 270 263 319 8.51e-17 81.6
Msa0324840 AT3G04670.1 57.895 57 24 0 214 270 263 319 1.17e-16 81.3
Msa0324840 AT4G24240.1 55.932 59 26 0 214 272 282 340 2.76e-16 80.1
Msa0324840 AT1G55600.1 42.697 89 46 2 191 275 281 368 3.12e-16 80.9
Msa0324840 AT1G29280.1 45.882 85 38 1 204 288 65 141 3.76e-16 78.6
Msa0324840 AT4G31550.2 44.872 78 40 1 214 291 246 320 4.38e-16 79.3
Msa0324840 AT4G31550.1 44.872 78 40 1 214 291 247 321 4.73e-16 79.3
Msa0324840 AT2G44745.1 43.956 91 46 2 184 270 112 201 4.86e-16 77.4
Msa0324840 AT2G24570.1 53.448 58 27 0 214 271 244 301 1.13e-15 78.2
Msa0324840 AT1G30650.1 34.884 129 74 2 146 273 158 277 1.64e-15 78.6
Msa0324840 AT2G34830.1 43.529 85 47 1 190 273 191 275 1.95e-15 78.2
Msa0324840 AT4G01250.1 48.649 74 38 0 210 283 125 198 2.18e-15 77.0
Msa0324840 AT2G34830.2 43.529 85 47 1 190 273 200 284 2.51e-15 78.2
Msa0324840 AT4G23550.1 43.373 83 47 0 211 293 138 220 2.84e-15 76.6
Msa0324840 AT3G58710.1 55.000 60 27 0 214 273 72 131 4.44e-15 75.5
Msa0324840 AT3G58710.2 55.000 60 27 0 214 273 71 130 4.50e-15 75.5
Msa0324840 AT5G52830.1 50.000 70 35 0 214 283 166 235 1.43e-14 75.1
Msa0324840 AT2G46130.2 42.105 95 47 3 189 283 6 92 3.33e-14 68.9
Msa0324840 AT4G39410.1 40.000 85 50 1 186 270 196 279 6.50e-14 72.8
Msa0324840 AT5G45050.2 36.842 114 69 1 214 324 1153 1266 1.46e-13 73.6
Msa0324840 AT5G45050.1 36.842 114 69 1 214 324 1181 1294 1.47e-13 73.6
Msa0324840 AT1G69310.3 41.667 84 44 2 188 271 122 200 1.53e-13 71.2
Msa0324840 AT5G45050.4 36.842 114 69 1 214 324 1042 1155 1.54e-13 73.6
Msa0324840 AT5G43290.1 47.541 61 31 1 214 274 115 174 3.16e-12 67.0
Msa0324840 AT4G31800.3 62.222 45 16 1 207 250 169 213 4.08e-12 66.2
Msa0324840 AT2G21900.1 46.667 60 31 1 214 273 110 168 5.77e-12 65.1
Msa0324840 AT2G46400.1 36.000 100 62 1 192 289 83 182 3.24e-11 64.3
Msa0324840 AT2G40740.1 43.902 82 35 3 214 284 174 255 3.91e-11 63.9
Msa0324840 AT2G40740.3 43.373 83 36 3 214 285 183 265 5.06e-11 63.9

Find 108 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATTGTTTATCATATT+TGG 0.055350 2_4:+30768706 None:intergenic
CACTACTCTTACTAGGTAAT+TGG 0.189983 2_4:+30768560 None:intergenic
ATTAATCACTTACTTGGATT+TGG 0.221328 2_4:+30771844 None:intergenic
AACACAAAGGCTACATAAAA+TGG 0.242040 2_4:-30772430 Msa0324840:exon
TTAATCACTTACTTGGATTT+GGG 0.256367 2_4:+30771845 None:intergenic
ATGCATAAACTATATATATA+AGG 0.261334 2_4:+30772492 None:intergenic
GAAAACTTACAGTATTCTTA+TGG 0.285405 2_4:+30772304 None:intergenic
TCTTCAGAATCGTTTACTTT+TGG 0.317553 2_4:-30771527 Msa0324840:CDS
AGCCTACTATCGTTGCAGTA+TGG 0.326812 2_4:-30770567 Msa0324840:CDS
GGGGATTTCCTTGAATTTGA+TGG 0.332291 2_4:+30771865 None:intergenic
AAAATCTGATCGTGCCCTTA+TGG 0.343745 2_4:+30770774 None:intergenic
GGGTGTCAATGGAGAAAATA+TGG 0.346609 2_4:-30770623 Msa0324840:CDS
TCTTTATTGTTATTATCATA+TGG 0.359665 2_4:+30772360 None:intergenic
TAGTTTACCTGCTTACGAAC+TGG 0.359755 2_4:+30770533 None:intergenic
GAAGTTGTGTTGTGTTATTA+AGG 0.369494 2_4:+30771889 None:intergenic
GATGATGTTAGGTTTGCTAT+TGG 0.371407 2_4:+30768994 None:intergenic
TCTTACTAGGTAATTGGCTT+TGG 0.372010 2_4:+30768566 None:intergenic
TGCATAAACTATATATATAA+GGG 0.377095 2_4:+30772493 None:intergenic
TTTGAGTTTGGTGGAGATAA+TGG 0.378048 2_4:+30768886 None:intergenic
AATTAACCACTACTCTTACT+AGG 0.379801 2_4:+30768553 None:intergenic
GTAGCTATAACATTTGAGTT+TGG 0.383227 2_4:+30768874 None:intergenic
TCAACAAATCTACAACAATA+TGG 0.389536 2_4:-30768943 Msa0324840:CDS
TTTAAGAGGTATGCTAAATC+TGG 0.393699 2_4:-30771569 Msa0324840:CDS
ATGATTATGGCTCCCTTCAT+AGG 0.402020 2_4:+30769038 None:intergenic
CAATGAGAGAAAACTTGCTT+TGG 0.409723 2_4:-30768692 Msa0324840:CDS
TGTTGAGTGCTGATGATGTT+AGG 0.413129 2_4:+30768983 None:intergenic
CAGCCAGCAGATTCAGTTGA+TGG 0.415892 2_4:+30772521 None:intergenic
AGTTCAAACAATGAAATTGC+TGG 0.417191 2_4:-30772400 Msa0324840:CDS
CGATAGTAGGCTCGTGGACA+AGG 0.430122 2_4:+30770578 None:intergenic
TTGCTGCACCGTTGTACAAT+GGG 0.433208 2_4:+30768504 None:intergenic
GTAAAATCAAGTGTAATTGT+AGG 0.435039 2_4:+30768829 None:intergenic
TGTAATGTTTAGATGGTTGA+TGG 0.440145 2_4:-30770644 Msa0324840:intron
GGAGAGCTAGAGTGTCCATA+AGG 0.442520 2_4:-30770789 Msa0324840:CDS
GGGATTTCCTTGAATTTGAT+GGG 0.451310 2_4:+30771866 None:intergenic
GCTATAACATTTGAGTTTGG+TGG 0.456982 2_4:+30768877 None:intergenic
TTGAATTGTAAATAGTTACT+TGG 0.461039 2_4:+30768799 None:intergenic
CTGGTGGAAGTTGATGATTA+TGG 0.462621 2_4:+30769025 None:intergenic
TGAAATAAATAGCAAAATAG+AGG 0.463782 2_4:-30770836 Msa0324840:CDS
AAGGAGAATGAAAATTTAAG+AGG 0.466438 2_4:-30771583 Msa0324840:CDS
TAATGACCTTGGAATGGAAA+AGG 0.467036 2_4:+30768751 None:intergenic
CAACTTCCCCATCAAATTCA+AGG 0.467550 2_4:-30771873 Msa0324840:CDS
AGATGGTTGATGGGTGTCAA+TGG 0.469133 2_4:-30770634 Msa0324840:CDS
GCCTACTATCGTTGCAGTAT+GGG 0.470435 2_4:-30770566 Msa0324840:CDS
TGCACCCAACCTCAGATGAT+TGG 0.470879 2_4:+30770812 None:intergenic
ACAGGTGCAAAGGTGTTTCA+AGG 0.472939 2_4:-30769082 Msa0324840:intron
GCCATGTCCTCGTTCACAAA+TGG 0.479556 2_4:-30768610 Msa0324840:CDS
TTTGGAAACACCTCAAATGA+TGG 0.495575 2_4:+30768724 None:intergenic
CCCCATACTGCAACGATAGT+AGG 0.503349 2_4:+30770565 None:intergenic
GAAGCTTGATGAAGCAAAGA+AGG 0.508309 2_4:-30771602 Msa0324840:CDS
AGGACCAATCATCTGAGGTT+GGG 0.511884 2_4:-30770816 Msa0324840:CDS
AGTTTACCTGCTTACGAACT+GGG 0.512250 2_4:+30770534 None:intergenic
GAGAGCTAGAGTGTCCATAA+GGG 0.513998 2_4:-30770788 Msa0324840:CDS
TTCATGCCCAGTTCGTAAGC+AGG 0.514284 2_4:-30770540 Msa0324840:intron
CTTAATAAAAGAGTAGATCC+TGG 0.516100 2_4:+30771918 None:intergenic
GTGTTCATCACAACCTATGA+AGG 0.519912 2_4:-30769051 Msa0324840:CDS
AGTATTCTTATGGAAGGAAG+AGG 0.520852 2_4:+30772314 None:intergenic
TAAAATCAAGTGTAATTGTA+GGG 0.521053 2_4:+30768830 None:intergenic
AAATAATCACAACTTGCTGC+TGG 0.523498 2_4:-30771464 Msa0324840:intron
GAGGACCAATCATCTGAGGT+TGG 0.528239 2_4:-30770817 Msa0324840:CDS
TCGTGGACAAGGGTTGCCCT+TGG 0.531338 2_4:+30770589 None:intergenic
ATTGTAGGGCATGTTGGAGA+TGG 0.532259 2_4:+30768844 None:intergenic
AGTGTAATTGTAGGGCATGT+TGG 0.533130 2_4:+30768838 None:intergenic
GTTCCATCAACTGAATCTGC+TGG 0.533550 2_4:-30772524 Msa0324840:five_prime_UTR
AATCATCTGAGGTTGGGTGC+AGG 0.533609 2_4:-30770810 Msa0324840:CDS
AATTGTAAATAGTTACTTGG+AGG 0.535746 2_4:+30768802 None:intergenic
TTGCTACAATCTGAAGGTGA+AGG 0.539687 2_4:+30770937 None:intergenic
GAGATAATGGTGAAGAGAAG+AGG 0.540861 2_4:+30768899 None:intergenic
TCATTACCCTCCATCATTTG+AGG 0.541576 2_4:-30768734 Msa0324840:CDS
ATGATGGAGGGTAATGACCT+TGG 0.552017 2_4:+30768740 None:intergenic
TTTGCTGCACCGTTGTACAA+TGG 0.552817 2_4:+30768503 None:intergenic
TCCATTTGTGAACGAGGACA+TGG 0.553076 2_4:+30768609 None:intergenic
ATTGTAAATAGTTACTTGGA+GGG 0.553408 2_4:+30768803 None:intergenic
GTAATGTTTAGATGGTTGAT+GGG 0.564516 2_4:-30770643 Msa0324840:intron
TGTAGATTTGTTGATGAGGT+AGG 0.565582 2_4:+30768952 None:intergenic
GATTTGTTGATGAGGTAGGA+AGG 0.569280 2_4:+30768956 None:intergenic
AACTGACGAGTAGGTAACAC+TGG 0.572998 2_4:+30770970 None:intergenic
ACTTACAGTATTCTTATGGA+AGG 0.574288 2_4:+30772308 None:intergenic
ATTGCAATTAACAGGTGCAA+AGG 0.574633 2_4:-30769092 Msa0324840:intron
AATGACCTTGGAATGGAAAA+GGG 0.575264 2_4:+30768752 None:intergenic
TAATCACTTACTTGGATTTG+GGG 0.580090 2_4:+30771846 None:intergenic
AATAATGCTTCCTTGAGAGA+AGG 0.583998 2_4:+30768637 None:intergenic
CCTACTATCGTTGCAGTATG+GGG 0.587972 2_4:-30770565 Msa0324840:CDS
TTATTGTTATTATCATATGG+TGG 0.589100 2_4:+30772363 None:intergenic
GCACCGTTGTACAATGGGAG+AGG 0.596900 2_4:+30768509 None:intergenic
GGAAACACCTCAAATGATGG+AGG 0.600501 2_4:+30768727 None:intergenic
GGAGGGTAATGACCTTGGAA+TGG 0.607233 2_4:+30768745 None:intergenic
GAAGGGAAATACACAAGATG+CGG 0.615352 2_4:-30770998 Msa0324840:CDS
TCATCAAAGAACTGACGAGT+AGG 0.619422 2_4:+30770961 None:intergenic
TATGGACAAAAGACAGCCAA+GGG 0.623915 2_4:-30770605 Msa0324840:CDS
ATATGGACAAAAGACAGCCA+AGG 0.629423 2_4:-30770606 Msa0324840:CDS
GCATGTGAGTACATTAATGA+AGG 0.632412 2_4:-30770859 Msa0324840:CDS
GAAACACCTCAAATGATGGA+GGG 0.632932 2_4:+30768728 None:intergenic
CTGCAACGATAGTAGGCTCG+TGG 0.637106 2_4:+30770572 None:intergenic
CAATTACCTAGTAAGAGTAG+TGG 0.638666 2_4:-30768559 Msa0324840:CDS
GATAGTAGGCTCGTGGACAA+GGG 0.644110 2_4:+30770579 None:intergenic
GCATAAACTATATATATAAG+GGG 0.644507 2_4:+30772494 None:intergenic
GGAAAACAATGAAAACAACA+TGG 0.645971 2_4:-30770893 Msa0324840:CDS
TGTTCATCACAACCTATGAA+GGG 0.646804 2_4:-30769050 Msa0324840:CDS
GGATTTCCTTGAATTTGATG+GGG 0.651214 2_4:+30771867 None:intergenic
AATAGAGGACCAATCATCTG+AGG 0.655874 2_4:-30770821 Msa0324840:CDS
AGAATCTCCATTTGTGAACG+AGG 0.664014 2_4:+30768603 None:intergenic
ATAGACTTAGCAAAGACTAG+TGG 0.669454 2_4:-30772556 Msa0324840:five_prime_UTR
GAAAACAATGAAAACAACAT+GGG 0.672904 2_4:-30770892 Msa0324840:CDS
TTGTTGTAGATTTGTTGATG+AGG 0.675654 2_4:+30768948 None:intergenic
AATTGCTGGAAGTTGATGAA+AGG 0.676322 2_4:-30772386 Msa0324840:CDS
GCTGCTCTATAGTTCATGTG+AGG 0.677528 2_4:+30772457 None:intergenic
GATCCTCTCCCATTGTACAA+CGG 0.679247 2_4:-30768512 Msa0324840:three_prime_UTR
TAGAGCAGCAATCAACACAA+AGG 0.685681 2_4:-30772443 Msa0324840:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAATCAAATATCAAATA+TGG + chr2_4:30768824-30768843 None:intergenic 10.0%
!!! ATTTTTGTATTTTATATTTG+TGG - chr2_4:30770756-30770775 Msa0324840:intron 10.0%
!!! TTTTATATGTATAATAGAAA+TGG - chr2_4:30770904-30770923 Msa0324840:CDS 10.0%
!!! ATCTATTTTCAAATATTATA+AGG - chr2_4:30771432-30771451 Msa0324840:intron 10.0%
!!! TCTATTTTCAAATATTATAA+GGG - chr2_4:30771433-30771452 Msa0324840:intron 10.0%
!! TGCATAAACTATATATATAA+GGG + chr2_4:30768578-30768597 None:intergenic 15.0%
!! ATGCATAAACTATATATATA+AGG + chr2_4:30768579-30768598 None:intergenic 15.0%
!!! TCTTTATTGTTATTATCATA+TGG + chr2_4:30768711-30768730 None:intergenic 15.0%
!!! GATAAATTAAACTCTAATTT+TGG - chr2_4:30769938-30769957 Msa0324840:intron 15.0%
!! TTTCTTCATAATTAATTAGA+AGG - chr2_4:30770052-30770071 Msa0324840:intron 15.0%
!! TTCTTCATAATTAATTAGAA+GGG - chr2_4:30770053-30770072 Msa0324840:intron 15.0%
!! CAAAATATAAGAATTTCAAT+TGG - chr2_4:30770674-30770693 Msa0324840:intron 15.0%
!!! CATTTTTAAGAATTTATGTT+GGG + chr2_4:30770717-30770736 None:intergenic 15.0%
!!! ACATTTTTAAGAATTTATGT+TGG + chr2_4:30770718-30770737 None:intergenic 15.0%
!!! AATTTTAATGCATTGAAAAA+GGG - chr2_4:30771027-30771046 Msa0324840:intron 15.0%
!!! AAAAGTTTTTGTAATGTAAT+TGG + chr2_4:30771086-30771105 None:intergenic 15.0%
!! TTAAATTTCTATCAAAGATA+TGG - chr2_4:30771139-30771158 Msa0324840:intron 15.0%
!!! ATTTTCAAATATTTCGAAAA+TGG - chr2_4:30771404-30771423 Msa0324840:intron 15.0%
!!! TTTTCAAATATTTCGAAAAT+GGG - chr2_4:30771405-30771424 Msa0324840:intron 15.0%
!! GCATAAACTATATATATAAG+GGG + chr2_4:30768577-30768596 None:intergenic 20.0%
!!! TTATTGTTATTATCATATGG+TGG + chr2_4:30768708-30768727 None:intergenic 20.0%
!!! TAATAACTTGACATTTTGAT+GGG - chr2_4:30769115-30769134 Msa0324840:intron 20.0%
!! AATAAGATTAATCACTTACT+TGG + chr2_4:30769233-30769252 None:intergenic 20.0%
!! ATTTAATGATCTCATGTATT+TGG - chr2_4:30769709-30769728 Msa0324840:intron 20.0%
!! TTTATCTATTTGTAAGTAGA+CGG + chr2_4:30769788-30769807 None:intergenic 20.0%
!!! TGTTGTTTATGTAATTAACA+TGG - chr2_4:30769864-30769883 Msa0324840:intron 20.0%
!!! TTAAACTCTAATTTTGGATT+TGG - chr2_4:30769944-30769963 Msa0324840:intron 20.0%
!!! TTACAAGTTGAAAATATCAT+AGG + chr2_4:30769997-30770016 None:intergenic 20.0%
!! TATGAAGAAAAAATGTACAA+AGG + chr2_4:30770042-30770061 None:intergenic 20.0%
!! TGAAATAAATAGCAAAATAG+AGG - chr2_4:30770232-30770251 Msa0324840:intron 20.0%
!! ATCAATTTGTAATGTTTAGA+TGG - chr2_4:30770417-30770436 Msa0324840:intron 20.0%
!! TAGGTTATATCATATCAAAA+TGG + chr2_4:30770583-30770602 None:intergenic 20.0%
!!! CAATTTTAATGCATTGAAAA+AGG - chr2_4:30771026-30771045 Msa0324840:intron 20.0%
!!! ATTTTAATGCATTGAAAAAG+GGG - chr2_4:30771028-30771047 Msa0324840:intron 20.0%
!! TAACCGAACAATAAATAAAA+TGG - chr2_4:30771288-30771307 Msa0324840:intron 20.0%
!!! CTTCCATTTTATTTATTGTT+CGG + chr2_4:30771294-30771313 None:intergenic 20.0%
!! TTTCAAATATTTCGAAAATG+GGG - chr2_4:30771406-30771425 Msa0324840:intron 20.0%
!!! AATTCTCATTTCATTCTTTT+AGG + chr2_4:30771634-30771653 None:intergenic 20.0%
!! AAAATTCAAGAACTAACTAT+TGG + chr2_4:30771693-30771712 None:intergenic 20.0%
!!! ATAATGATGAGACTATTTTA+AGG - chr2_4:30771860-30771879 Msa0324840:CDS 20.0%
!!! TGTGTAATTCTGTTTTAAAA+TGG - chr2_4:30771896-30771915 Msa0324840:CDS 20.0%
!! TTAATTACATTGCAATTAAC+AGG - chr2_4:30771968-30771987 Msa0324840:intron 20.0%
!! TAAAATCAAGTGTAATTGTA+GGG + chr2_4:30772241-30772260 None:intergenic 20.0%
!! TTGAATTGTAAATAGTTACT+TGG + chr2_4:30772272-30772291 None:intergenic 20.0%
!! AGTGGTTAATTATAAAAAGT+TGG - chr2_4:30772527-30772546 Msa0324840:five_prime_UTR 20.0%
!! GTGGTTAATTATAAAAAGTT+GGG - chr2_4:30772528-30772547 Msa0324840:five_prime_UTR 20.0%
! GAAAACTTACAGTATTCTTA+TGG + chr2_4:30768767-30768786 None:intergenic 25.0%
!! AAGAGTCATATACTTTGTTT+AGG - chr2_4:30768914-30768933 Msa0324840:CDS 25.0%
! TTAGCTTATGATGAAAATCA+TGG - chr2_4:30769021-30769040 Msa0324840:CDS 25.0%
! TAGCTTATGATGAAAATCAT+GGG - chr2_4:30769022-30769041 Msa0324840:CDS 25.0%
! AAATGTCAAGTTATTAGGTT+AGG + chr2_4:30769111-30769130 None:intergenic 25.0%
! CATCAAAATGTCAAGTTATT+AGG + chr2_4:30769116-30769135 None:intergenic 25.0%
!! CTAATAACTTGACATTTTGA+TGG - chr2_4:30769114-30769133 Msa0324840:intron 25.0%
! TTAATCACTTACTTGGATTT+GGG + chr2_4:30769226-30769245 None:intergenic 25.0%
! ATTAATCACTTACTTGGATT+TGG + chr2_4:30769227-30769246 None:intergenic 25.0%
!!! TTTGATAATTGCTCTAAGAT+TGG - chr2_4:30769431-30769450 Msa0324840:intron 25.0%
! AAGGAGAATGAAAATTTAAG+AGG - chr2_4:30769485-30769504 Msa0324840:intron 25.0%
! AAATGAGTGTGAATCAAATA+AGG + chr2_4:30769661-30769680 None:intergenic 25.0%
! TTATCTATTTGTAAGTAGAC+GGG + chr2_4:30769787-30769806 None:intergenic 25.0%
!!! TTTTTTTTGCTACAATCTGA+AGG + chr2_4:30770140-30770159 None:intergenic 25.0%
! TCAGATTGTAGCAAAAAAAA+TGG - chr2_4:30770140-30770159 Msa0324840:intron 25.0%
! GAAAACAATGAAAACAACAT+GGG - chr2_4:30770176-30770195 Msa0324840:intron 25.0%
!!! TCGAACTTTTGATAGTTTTT+AGG + chr2_4:30770602-30770621 None:intergenic 25.0%
!! GTTCAATTGATAATATAGCA+AGG - chr2_4:30771200-30771219 Msa0324840:intron 25.0%
! ATGTAATGAATGTTTGTGAA+AGG - chr2_4:30771318-30771337 Msa0324840:intron 25.0%
! TTCCTTATAATGCTAATTAG+TGG + chr2_4:30771555-30771574 None:intergenic 25.0%
! ATTATAAGGAAGATGATGAT+GGG - chr2_4:30771564-30771583 Msa0324840:CDS 25.0%
! AAAGTCATGAATACATCATA+AGG - chr2_4:30771836-30771855 Msa0324840:intron 25.0%
! TCAACAAATCTACAACAATA+TGG - chr2_4:30772125-30772144 Msa0324840:intron 25.0%
! GTAAAATCAAGTGTAATTGT+AGG + chr2_4:30772242-30772261 None:intergenic 25.0%
! ATTGTAAATAGTTACTTGGA+GGG + chr2_4:30772268-30772287 None:intergenic 25.0%
! AATTGTAAATAGTTACTTGG+AGG + chr2_4:30772269-30772288 None:intergenic 25.0%
! CTCTCATTGTTTATCATATT+TGG + chr2_4:30772365-30772384 None:intergenic 25.0%
AACACAAAGGCTACATAAAA+TGG - chr2_4:30768638-30768657 Msa0324840:CDS 30.0%
AGTTCAAACAATGAAATTGC+TGG - chr2_4:30768668-30768687 Msa0324840:CDS 30.0%
ACTTACAGTATTCTTATGGA+AGG + chr2_4:30768763-30768782 None:intergenic 30.0%
TAATCCAGTATACACAAACA+TGG + chr2_4:30768797-30768816 None:intergenic 30.0%
CTTAATAAAAGAGTAGATCC+TGG + chr2_4:30769153-30769172 None:intergenic 30.0%
! GAAGTTGTGTTGTGTTATTA+AGG + chr2_4:30769182-30769201 None:intergenic 30.0%
TAATCACTTACTTGGATTTG+GGG + chr2_4:30769225-30769244 None:intergenic 30.0%
! TTTAAGAGGTATGCTAAATC+TGG - chr2_4:30769499-30769518 Msa0324840:intron 30.0%
! TCTTCAGAATCGTTTACTTT+TGG - chr2_4:30769541-30769560 Msa0324840:intron 30.0%
!! AGCAAGTTGTGATTATTTTG+AGG + chr2_4:30769602-30769621 None:intergenic 30.0%
!!! AATTTTGGATTTGGTGAATG+TGG - chr2_4:30769953-30769972 Msa0324840:intron 30.0%
! AAAAAATGGATTTGCCATAG+TGG - chr2_4:30770154-30770173 Msa0324840:intron 30.0%
! GTTTTCATTGTTTTCCACTA+TGG + chr2_4:30770171-30770190 None:intergenic 30.0%
GGAAAACAATGAAAACAACA+TGG - chr2_4:30770175-30770194 Msa0324840:intron 30.0%
! GATCAGATTTTGCATTTGTA+AGG - chr2_4:30770306-30770325 Msa0324840:intron 30.0%
TGTAATGTTTAGATGGTTGA+TGG - chr2_4:30770424-30770443 Msa0324840:intron 30.0%
GTAATGTTTAGATGGTTGAT+GGG - chr2_4:30770425-30770444 Msa0324840:intron 30.0%
! TCGATTTTATAGAAGTGAGT+TGG - chr2_4:30770643-30770662 Msa0324840:intron 30.0%
! CGGACAAACTATTAATTTGA+GGG - chr2_4:30770816-30770835 Msa0324840:CDS 30.0%
! GGACAAACTATTAATTTGAG+GGG - chr2_4:30770817-30770836 Msa0324840:CDS 30.0%
!! GCAAATATGTTACTTTGTTC+TGG - chr2_4:30770960-30770979 Msa0324840:CDS 30.0%
TTGTAGTGCATCATCAATTA+AGG + chr2_4:30770993-30771012 None:intergenic 30.0%
! GACTATTAGTTCTTACTAGA+TGG - chr2_4:30771164-30771183 Msa0324840:intron 30.0%
! TATTAGTTCTTACTAGATGG+AGG - chr2_4:30771167-30771186 Msa0324840:intron 30.0%
! ATTGATAATATAGCAAGGTC+TGG - chr2_4:30771205-30771224 Msa0324840:intron 30.0%
! TTGATAATATAGCAAGGTCT+GGG - chr2_4:30771206-30771225 Msa0324840:intron 30.0%
ATGAATGTTTGTGAAAGGAA+AGG - chr2_4:30771323-30771342 Msa0324840:intron 30.0%
!! AGGGTATTGTTTCTCATATT+AGG - chr2_4:30771452-30771471 Msa0324840:intron 30.0%
! GGGTATTGTTTCTCATATTA+GGG - chr2_4:30771453-30771472 Msa0324840:intron 30.0%
!! TGTTCTAAAGACACTCTTTA+TGG - chr2_4:30771490-30771509 Msa0324840:CDS 30.0%
TGCCACTAATTAGCATTATA+AGG - chr2_4:30771550-30771569 Msa0324840:CDS 30.0%
CATTATAAGGAAGATGATGA+TGG - chr2_4:30771563-30771582 Msa0324840:CDS 30.0%
!!! TTCTTTTAGGTTGAATGTGT+AGG + chr2_4:30771621-30771640 None:intergenic 30.0%
ATTCAAGAACTAACTATTGG+AGG + chr2_4:30771690-30771709 None:intergenic 30.0%
! GTAAATGAGACAAGTTTTCA+TGG + chr2_4:30771792-30771811 None:intergenic 30.0%
!! TTGTTGTAGATTTGTTGATG+AGG + chr2_4:30772123-30772142 None:intergenic 30.0%
! GTAGCTATAACATTTGAGTT+TGG + chr2_4:30772197-30772216 None:intergenic 30.0%
AATTAACCACTACTCTTACT+AGG + chr2_4:30772518-30772537 None:intergenic 30.0%
ATAGACTTAGCAAAGACTAG+TGG - chr2_4:30768512-30768531 Msa0324840:three_prime_UTR 35.0%
!! AATTGCTGGAAGTTGATGAA+AGG - chr2_4:30768682-30768701 Msa0324840:CDS 35.0%
AGTATTCTTATGGAAGGAAG+AGG + chr2_4:30768757-30768776 None:intergenic 35.0%
TATGCCATGTTTGTGTATAC+TGG - chr2_4:30768790-30768809 Msa0324840:CDS 35.0%
TATGATGAAAATCATGGGTG+TGG - chr2_4:30769027-30769046 Msa0324840:CDS 35.0%
GGATTTCCTTGAATTTGATG+GGG + chr2_4:30769204-30769223 None:intergenic 35.0%
GGGATTTCCTTGAATTTGAT+GGG + chr2_4:30769205-30769224 None:intergenic 35.0%
!!! GTTGTGATTATTTTGAGGCA+AGG + chr2_4:30769597-30769616 None:intergenic 35.0%
!! AAATAATCACAACTTGCTGC+TGG - chr2_4:30769604-30769623 Msa0324840:intron 35.0%
TCGACAACACTACAACATAT+AGG + chr2_4:30769890-30769909 None:intergenic 35.0%
TAGTGTTGTCGATCATCAAT+TGG - chr2_4:30769898-30769917 Msa0324840:intron 35.0%
GCATGTGAGTACATTAATGA+AGG - chr2_4:30770209-30770228 Msa0324840:intron 35.0%
! GCGGACAAACTATTAATTTG+AGG - chr2_4:30770815-30770834 Msa0324840:CDS 35.0%
TTAATGCATTGAAAAAGGGG+AGG - chr2_4:30771031-30771050 Msa0324840:intron 35.0%
! TTCCAAAGCACTTCAAACTT+AGG - chr2_4:30771058-30771077 Msa0324840:intron 35.0%
TGATAATATAGCAAGGTCTG+GGG - chr2_4:30771207-30771226 Msa0324840:intron 35.0%
!!! CATCTTTTTTGGTTGTGTTC+GGG + chr2_4:30771239-30771258 None:intergenic 35.0%
!!! CCATCTTTTTTGGTTGTGTT+CGG + chr2_4:30771240-30771259 None:intergenic 35.0%
AACACAACCAAAAAAGATGG+AGG - chr2_4:30771240-30771259 Msa0324840:intron 35.0%
!!! ACTTTCACCTCCATCTTTTT+TGG + chr2_4:30771250-30771269 None:intergenic 35.0%
AATGTTTGTGAAAGGAAAGG+TGG - chr2_4:30771326-30771345 Msa0324840:intron 35.0%
ATGTTTGTGAAAGGAAAGGT+GGG - chr2_4:30771327-30771346 Msa0324840:intron 35.0%
ATTGCAATTAACAGGTGCAA+AGG - chr2_4:30771976-30771995 Msa0324840:intron 35.0%
TGTTCATCACAACCTATGAA+GGG - chr2_4:30772018-30772037 Msa0324840:intron 35.0%
!! GATGATGTTAGGTTTGCTAT+TGG + chr2_4:30772077-30772096 None:intergenic 35.0%
!! TGTAGATTTGTTGATGAGGT+AGG + chr2_4:30772119-30772138 None:intergenic 35.0%
! TTTGAGTTTGGTGGAGATAA+TGG + chr2_4:30772185-30772204 None:intergenic 35.0%
! GCTATAACATTTGAGTTTGG+TGG + chr2_4:30772194-30772213 None:intergenic 35.0%
AATGACCTTGGAATGGAAAA+GGG + chr2_4:30772319-30772338 None:intergenic 35.0%
TAATGACCTTGGAATGGAAA+AGG + chr2_4:30772320-30772339 None:intergenic 35.0%
TTTGGAAACACCTCAAATGA+TGG + chr2_4:30772347-30772366 None:intergenic 35.0%
! CAATGAGAGAAAACTTGCTT+TGG - chr2_4:30772376-30772395 Msa0324840:CDS 35.0%
AGAAAAAGATCCTTCTCTCA+AGG - chr2_4:30772421-30772440 Msa0324840:exon 35.0%
AATAATGCTTCCTTGAGAGA+AGG + chr2_4:30772434-30772453 None:intergenic 35.0%
! TCTTACTAGGTAATTGGCTT+TGG + chr2_4:30772505-30772524 None:intergenic 35.0%
CACTACTCTTACTAGGTAAT+TGG + chr2_4:30772511-30772530 None:intergenic 35.0%
CAATTACCTAGTAAGAGTAG+TGG - chr2_4:30772509-30772528 Msa0324840:five_prime_UTR 35.0%
TAGAGCAGCAATCAACACAA+AGG - chr2_4:30768625-30768644 Msa0324840:CDS 40.0%
TGTATGAATCGAGATCATCG+AGG + chr2_4:30768848-30768867 None:intergenic 40.0%
CAACTTCCCCATCAAATTCA+AGG - chr2_4:30769195-30769214 Msa0324840:intron 40.0%
GGGGATTTCCTTGAATTTGA+TGG + chr2_4:30769206-30769225 None:intergenic 40.0%
GAAGCTTGATGAAGCAAAGA+AGG - chr2_4:30769466-30769485 Msa0324840:intron 40.0%
GAAGGGAAATACACAAGATG+CGG - chr2_4:30770070-30770089 Msa0324840:intron 40.0%
TCATCAAAGAACTGACGAGT+AGG + chr2_4:30770110-30770129 None:intergenic 40.0%
TTGCTACAATCTGAAGGTGA+AGG + chr2_4:30770134-30770153 None:intergenic 40.0%
AATAGAGGACCAATCATCTG+AGG - chr2_4:30770247-30770266 Msa0324840:intron 40.0%
AAAATCTGATCGTGCCCTTA+TGG + chr2_4:30770297-30770316 None:intergenic 40.0%
GGGTGTCAATGGAGAAAATA+TGG - chr2_4:30770445-30770464 Msa0324840:intron 40.0%
ATATGGACAAAAGACAGCCA+AGG - chr2_4:30770462-30770481 Msa0324840:intron 40.0%
TATGGACAAAAGACAGCCAA+GGG - chr2_4:30770463-30770482 Msa0324840:intron 40.0%
AGTTTACCTGCTTACGAACT+GGG + chr2_4:30770537-30770556 None:intergenic 40.0%
TAGTTTACCTGCTTACGAAC+TGG + chr2_4:30770538-30770557 None:intergenic 40.0%
CCTCTCTATTGAGAATGATG+CGG - chr2_4:30770796-30770815 Msa0324840:CDS 40.0%
! AGCCTAAGTTTGAAGTGCTT+TGG + chr2_4:30771063-30771082 None:intergenic 40.0%
CCGAACACAACCAAAAAAGA+TGG - chr2_4:30771237-30771256 Msa0324840:intron 40.0%
!! TAAGGAAGATGATGATGGGA+AGG - chr2_4:30771568-30771587 Msa0324840:CDS 40.0%
GTGTTCATCACAACCTATGA+AGG - chr2_4:30772017-30772036 Msa0324840:intron 40.0%
ATGATTATGGCTCCCTTCAT+AGG + chr2_4:30772033-30772052 None:intergenic 40.0%
!! CTGGTGGAAGTTGATGATTA+TGG + chr2_4:30772046-30772065 None:intergenic 40.0%
!!! TTTGCTATTGGTTTTGCTGC+TGG + chr2_4:30772065-30772084 None:intergenic 40.0%
!! TGTTGAGTGCTGATGATGTT+AGG + chr2_4:30772088-30772107 None:intergenic 40.0%
!! GATTTGTTGATGAGGTAGGA+AGG + chr2_4:30772115-30772134 None:intergenic 40.0%
! GAGATAATGGTGAAGAGAAG+AGG + chr2_4:30772172-30772191 None:intergenic 40.0%
AGTGTAATTGTAGGGCATGT+TGG + chr2_4:30772233-30772252 None:intergenic 40.0%
TCATTACCCTCCATCATTTG+AGG - chr2_4:30772334-30772353 Msa0324840:CDS 40.0%
! GAAACACCTCAAATGATGGA+GGG + chr2_4:30772343-30772362 None:intergenic 40.0%
AGAATCTCCATTTGTGAACG+AGG + chr2_4:30772468-30772487 None:intergenic 40.0%
GTTCCATCAACTGAATCTGC+TGG - chr2_4:30768544-30768563 Msa0324840:exon 45.0%
GCTGCTCTATAGTTCATGTG+AGG + chr2_4:30768614-30768633 None:intergenic 45.0%
AACTGACGAGTAGGTAACAC+TGG + chr2_4:30770101-30770120 None:intergenic 45.0%
! AGGACCAATCATCTGAGGTT+GGG - chr2_4:30770252-30770271 Msa0324840:intron 45.0%
! GAGAGCTAGAGTGTCCATAA+GGG - chr2_4:30770280-30770299 Msa0324840:intron 45.0%
!! AGATGGTTGATGGGTGTCAA+TGG - chr2_4:30770434-30770453 Msa0324840:intron 45.0%
AGCCTACTATCGTTGCAGTA+TGG - chr2_4:30770501-30770520 Msa0324840:intron 45.0%
GCCTACTATCGTTGCAGTAT+GGG - chr2_4:30770502-30770521 Msa0324840:intron 45.0%
CCTACTATCGTTGCAGTATG+GGG - chr2_4:30770503-30770522 Msa0324840:intron 45.0%
CCGCATCATTCTCAATAGAG+AGG + chr2_4:30770799-30770818 None:intergenic 45.0%
!! ACAGGTGCAAAGGTGTTTCA+AGG - chr2_4:30771986-30772005 Msa0324840:intron 45.0%
ATTGTAGGGCATGTTGGAGA+TGG + chr2_4:30772227-30772246 None:intergenic 45.0%
! TTGCTCCCTTTTCCATTCCA+AGG - chr2_4:30772311-30772330 Msa0324840:intron 45.0%
! ATGATGGAGGGTAATGACCT+TGG + chr2_4:30772331-30772350 None:intergenic 45.0%
GGAAACACCTCAAATGATGG+AGG + chr2_4:30772344-30772363 None:intergenic 45.0%
TCCATTTGTGAACGAGGACA+TGG + chr2_4:30772462-30772481 None:intergenic 45.0%
GATCCTCTCCCATTGTACAA+CGG - chr2_4:30772556-30772575 Msa0324840:five_prime_UTR 45.0%
CAGCCAGCAGATTCAGTTGA+TGG + chr2_4:30768550-30768569 None:intergenic 50.0%
GATGGGAAAATCATGCAGCC+AGG - chr2_4:30769132-30769151 Msa0324840:intron 50.0%
! GAGGACCAATCATCTGAGGT+TGG - chr2_4:30770251-30770270 Msa0324840:intron 50.0%
TGCACCCAACCTCAGATGAT+TGG + chr2_4:30770259-30770278 None:intergenic 50.0%
! AATCATCTGAGGTTGGGTGC+AGG - chr2_4:30770258-30770277 Msa0324840:intron 50.0%
! GGAGAGCTAGAGTGTCCATA+AGG - chr2_4:30770279-30770298 Msa0324840:intron 50.0%
GATAGTAGGCTCGTGGACAA+GGG + chr2_4:30770492-30770511 None:intergenic 50.0%
CCCCATACTGCAACGATAGT+AGG + chr2_4:30770506-30770525 None:intergenic 50.0%
TTCATGCCCAGTTCGTAAGC+AGG - chr2_4:30770528-30770547 Msa0324840:intron 50.0%
!!! GCTATTGGTTTTGCTGCTGG+TGG + chr2_4:30772062-30772081 None:intergenic 50.0%
GGAGGGTAATGACCTTGGAA+TGG + chr2_4:30772326-30772345 None:intergenic 50.0%
GCCATGTCCTCGTTCACAAA+TGG - chr2_4:30772458-30772477 Msa0324840:five_prime_UTR 50.0%
CGATAGTAGGCTCGTGGACA+AGG + chr2_4:30770493-30770512 None:intergenic 55.0%
CTGCAACGATAGTAGGCTCG+TGG + chr2_4:30770499-30770518 None:intergenic 55.0%
GGAAAGGTGGGAAGCGAACT+AGG - chr2_4:30771339-30771358 Msa0324840:intron 55.0%
GCACCGTTGTACAATGGGAG+AGG + chr2_4:30772562-30772581 None:intergenic 55.0%
TCGTGGACAAGGGTTGCCCT+TGG + chr2_4:30770482-30770501 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 30768507 30772583 30768507 ID=Msa0324840;Name=Msa0324840
chr2_4 mRNA 30768507 30772583 30768507 ID=Msa0324840-mRNA-1;Parent=Msa0324840;Name=Msa0324840-mRNA-1;_AED=0.03;_eAED=0.03;_QI=150|1|1|1|1|1|6|48|430
chr2_4 exon 30768507 30769100 30768507 ID=Msa0324840-mRNA-1:exon:10310;Parent=Msa0324840-mRNA-1
chr2_4 exon 30770541 30770654 30770541 ID=Msa0324840-mRNA-1:exon:10309;Parent=Msa0324840-mRNA-1
chr2_4 exon 30770768 30771019 30770768 ID=Msa0324840-mRNA-1:exon:10308;Parent=Msa0324840-mRNA-1
chr2_4 exon 30771465 30771641 30771465 ID=Msa0324840-mRNA-1:exon:10307;Parent=Msa0324840-mRNA-1
chr2_4 exon 30771857 30771940 30771857 ID=Msa0324840-mRNA-1:exon:10306;Parent=Msa0324840-mRNA-1
chr2_4 exon 30772314 30772583 30772314 ID=Msa0324840-mRNA-1:exon:10305;Parent=Msa0324840-mRNA-1
chr2_4 five_prime_UTR 30772434 30772583 30772434 ID=Msa0324840-mRNA-1:five_prime_utr;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30772314 30772433 30772314 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30771857 30771940 30771857 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30771465 30771641 30771465 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30770768 30771019 30770768 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30770541 30770654 30770541 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 CDS 30768555 30769100 30768555 ID=Msa0324840-mRNA-1:cds;Parent=Msa0324840-mRNA-1
chr2_4 three_prime_UTR 30768507 30768554 30768507 ID=Msa0324840-mRNA-1:three_prime_utr;Parent=Msa0324840-mRNA-1
Gene Sequence

>Msa0324840

ATGGAAGAAAAAGTTCAAACAATGAAATTGCTGGAAGTTGATGAAAGGCCACCATATGATAATAACAATAAAGATCTACAAAACAAGATCGATGAATCCTCTTCCTTCCATAAGAATACTCCAGGATCTACTCTTTTATTAAGTTTGAGCCTTAATAACACAACACAACTTCCCCATCAAATTCAAGGAAATCCCCAAATCCAAATTGGCAATATACGAGTGAAGCTTGATGAAGCAAAGAAGGAGAATGAAAATTTAAGAGGTATGCTAAATCTGGTCAACGATCGATGCAATGTTCTTCAGAATCGTTTACTTTTGGCTATGCATATGCATATGCATCAATCATCTTCCTTGCCTCAAAATAATCACAACTTGCTGCTGAAGGGAAATACACAAGATGCGGAGAAACCAGTGTTACCTACTCGTCAGTTCTTTGATGAGCCTTCACCTTCAGATTGTAGCAAAAAAAATGGATTTGCCATAGTGGAAAACAATGAAAACAACATGGGAAGAAATCTTGCATGTGAGTACATTAATGAAGGTGAAATAAATAGCAAAATAGAGGACCAATCATCTGAGGTTGGGTGCAGGAGAGCTAGAGTGTCCATAAGGGCACGATCAGATTTTGCATTTATGGTTGATGGGTGTCAATGGAGAAAATATGGACAAAAGACAGCCAAGGGCAACCCTTGTCCACGAGCCTACTATCGTTGCAGTATGGGGACTTCATGCCCAGTTCGTAAGCAGGTGCAAAGGTGTTTCAAGGATGAGAGTGTGTTCATCACAACCTATGAAGGGAGCCATAATCATCAACTTCCACCAGCAGCAAAACCAATAGCAAACCTAACATCATCAGCACTCAACACATTCCTTCCTACCTCATCAACAAATCTACAACAATATGGCAACAATCTAACAAATACATTCCTCTTCTCTTCACCATTATCTCCACCAAACTCAAATGTTATAGCTACTTTCTCTCCATCTCCAACATGCCCTACAATTACACTTGATTTTACACTCCCTCCAAGTAACTATTTACAATTCAAAAATCATAAACAATCATCATTGCTCCCTTTTCCATTCCAAGGTCATTACCCTCCATCATTTGAGGTGTTTCCAAATATGATAAACAATGAGAGAAAACTTGCTTTGGTTGATGTTGTGAGTGAAGCTCTAGAAAAAGATCCTTCTCTCAAGGAAGCATTATTTTCAGCCATGTCCTCGTTCACAAATGGAGATTCTTCAAATATTAATAACCAAAGCCAATTACCTAGTAAGAGTAGTGGTTAA
Protein sequence

>Msa0324840

MEEKVQTMKLLEVDERPPYDNNNKDLQNKIDESSSFHKNTPGSTLLLSLSLNNTTQLPHQIQGNPQIQIGNIRVKLDEAKKENENLRGMLNLVNDRCNVLQNRLLLAMHMHMHQSSSLPQNNHNLLLKGNTQDAEKPVLPTRQFFDEPSPSDCSKKNGFAIVENNENNMGRNLACEYINEGEINSKIEDQSSEVGCRRARVSIRARSDFAFMVDGCQWRKYGQKTAKGNPCPRAYYRCSMGTSCPVRKQVQRCFKDESVFITTYEGSHNHQLPPAAKPIANLTSSALNTFLPTSSTNLQQYGNNLTNTFLFSSPLSPPNSNVIATFSPSPTCPTITLDFTLPPSNYLQFKNHKQSSLLPFPFQGHYPPSFEVFPNMINNERKLALVDVVSEALEKDPSLKEALFSAMSSFTNGDSSNINNQSQLPSKSSG*