AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0409270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0409270 MtrunA17_Chr4g0027321 54.598 174 62 6 60 227 130 292 4.98e-49 162
Msa0409270 MtrunA17_Chr3g0119711 38.835 206 105 9 33 227 3 198 1.90e-30 111
Msa0409270 MtrunA17_Chr3g0120641 36.364 220 116 8 28 227 26 241 5.41e-21 87.8
Msa0409270 MtrunA17_Chr3g0120941 36.041 197 100 10 44 227 59 242 2.61e-18 80.9
Msa0409270 MtrunA17_Chr3g0120951 36.686 169 85 9 68 227 51 206 8.24e-18 78.6
Msa0409270 MtrunA17_Chr3g0120591 34.615 208 123 6 30 227 18 222 1.15e-17 78.6
Msa0409270 MtrunA17_Chr3g0120961 37.179 156 71 8 74 221 47 183 2.30e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATGACAGCTTTCCATTT+TGG 0.200035 3_2:-49789629 Msa0409270:CDS
CACTAATGTTTCTGTATAAT+TGG 0.200274 3_2:-49788861 Msa0409270:three_prime_UTR
GCTTTAAAAGTAATGTAATA+TGG 0.262097 3_2:+49789117 None:intergenic
GATTTCAGCCGTTTCGATTT+CGG 0.272727 3_2:+49789834 None:intergenic
TGGTTCTTTGTATACGAATA+AGG 0.284069 3_2:-49789592 Msa0409270:CDS
GAATCTTCTGGATCCGGTTT+CGG 0.312704 3_2:+49789693 None:intergenic
GGTTATGGTTATGCTTGTTT+TGG 0.320799 3_2:-49788969 Msa0409270:three_prime_UTR
TCACGACGCTTATACCAAAA+TGG 0.333746 3_2:+49789615 None:intergenic
TCACTATACTCAGAATCTTC+TGG 0.339930 3_2:+49789681 None:intergenic
GAAATCGAAACGGCTGAAAT+CGG 0.340115 3_2:-49789832 Msa0409270:CDS
AATTAATTAAATAAATATGA+TGG 0.373275 3_2:-49788918 Msa0409270:three_prime_UTR
AATACACACCGCCATGGTTA+TGG 0.393191 3_2:-49788984 Msa0409270:three_prime_UTR
ACTTTATCAAACACAAAAGT+TGG 0.407632 3_2:+49789186 None:intergenic
ATGAAACACTCTTTGACAAC+AGG 0.409120 3_2:+49789018 None:intergenic
GTGTTTCAAAATGTCGAAAT+CGG 0.413339 3_2:-49789865 Msa0409270:exon
GCTGAAATCGGAACCGAAAC+CGG 0.414648 3_2:-49789706 Msa0409270:CDS
AAATCGAAACGGCTGAAATC+GGG 0.418006 3_2:-49789831 Msa0409270:CDS
CTACTTCTTTATATGCTCCT+AGG 0.435411 3_2:-49789140 Msa0409270:CDS
ATCTGTGGAGGAGGAGGAGG+AGG 0.436107 3_2:-49789736 Msa0409270:CDS
TACTCAGAATCTTCTGGATC+CGG 0.439841 3_2:+49789687 None:intergenic
TGCAGAGGGGTTTGAGAGTA+CGG 0.440533 3_2:+49789343 None:intergenic
TAAGGCTTGGAGGGATCGTC+AGG 0.442047 3_2:-49789574 Msa0409270:CDS
GGAGGAGAAACGGCTGAAAT+CGG 0.450703 3_2:-49789718 Msa0409270:CDS
AGAAAGAAATGAAACTCTAA+AGG 0.454475 3_2:+49788793 None:intergenic
TTCATCTGTGGAGGAGGAGG+AGG 0.455235 3_2:-49789739 Msa0409270:CDS
CAAAATGTCGAAATCGGAAC+AGG 0.457893 3_2:-49789859 Msa0409270:exon
CACTGTCCACCACGTTGTGC+TGG 0.467693 3_2:-49789402 Msa0409270:CDS
TACGCTGCAATACATGAGTC+TGG 0.467899 3_2:+49789052 None:intergenic
AAAGTAATGTAATATGGCCT+AGG 0.487724 3_2:+49789123 None:intergenic
TAAAGCAAAAGCAGAAGCAA+AGG 0.500294 3_2:-49789100 Msa0409270:CDS
ATTAATTAAATAAATATGAT+GGG 0.503492 3_2:-49788917 Msa0409270:three_prime_UTR
CAATCAAACGAATATTAAGC+AGG 0.504217 3_2:-49789301 Msa0409270:intron
TGAGGATGAGGAGGATAAGC+AGG 0.508361 3_2:-49789778 Msa0409270:CDS
ACACGATGAAGATTCATATG+AGG 0.514467 3_2:-49789796 Msa0409270:CDS
CTTTGTATACGAATAAGGCT+TGG 0.515687 3_2:-49789587 Msa0409270:CDS
AGATTCATCTGTGGAGGAGG+AGG 0.522683 3_2:-49789742 Msa0409270:CDS
GTCTGGAAAGTTGTTAGAGC+AGG 0.523457 3_2:+49789069 None:intergenic
GTTGTCAAGTGTACCCATCC+TGG 0.532074 3_2:-49789165 Msa0409270:CDS
TGTATACGAATAAGGCTTGG+AGG 0.537395 3_2:-49789584 Msa0409270:CDS
AAAGCAAAAGCAGAAGCAAA+GGG 0.538089 3_2:-49789099 Msa0409270:CDS
ACAAGATTCATCTGTGGAGG+AGG 0.546974 3_2:-49789745 Msa0409270:CDS
TGAAGATTCATATGAGGATG+AGG 0.548643 3_2:-49789790 Msa0409270:CDS
CACATCATCGCTTAAGTCGA+GGG 0.551344 3_2:+49789368 None:intergenic
AACAGGAACCGAAATCGAAA+CGG 0.558710 3_2:-49789842 Msa0409270:CDS
CTTCAAAGCAGCATTGCAGA+GGG 0.559702 3_2:+49789329 None:intergenic
GCATATAAAGAAGTAGCACC+AGG 0.567371 3_2:+49789147 None:intergenic
TGTCCACCACGTTGTGCTGG+CGG 0.572730 3_2:-49789399 Msa0409270:CDS
CATTAGTGACATAAATAACA+TGG 0.577507 3_2:+49788876 None:intergenic
GTATACGAATAAGGCTTGGA+GGG 0.581642 3_2:-49789583 Msa0409270:CDS
TAAAGAAGTAGCACCAGGAT+GGG 0.590468 3_2:+49789152 None:intergenic
GCACATCATCGCTTAAGTCG+AGG 0.599300 3_2:+49789367 None:intergenic
ATAAAGAAGTAGCACCAGGA+TGG 0.610411 3_2:+49789151 None:intergenic
CAGCACAACGTGGTGGACAG+TGG 0.610706 3_2:+49789403 None:intergenic
GAAACAAGATTCATCTGTGG+AGG 0.613961 3_2:-49789748 Msa0409270:CDS
AAATCTAATACACACCGCCA+TGG 0.616867 3_2:-49788990 Msa0409270:exon
TCTTCAAAGCAGCATTGCAG+AGG 0.630589 3_2:+49789328 None:intergenic
AGATTCATATGAGGATGAGG+AGG 0.633765 3_2:-49789787 Msa0409270:CDS
GAAACGGCTGAAATCGGGAT+CGG 0.652067 3_2:-49789826 Msa0409270:CDS
AGAAGATTCTGAGTATAGTG+AGG 0.652619 3_2:-49789679 Msa0409270:CDS
AACAAGCATAACCATAACCA+TGG 0.658373 3_2:+49788973 None:intergenic
GCAAAAGCAGAAGCAAAGGG+AGG 0.667232 3_2:-49789096 Msa0409270:CDS
ACTACACGTAAGTCTTGCCA+AGG 0.669722 3_2:+49788942 None:intergenic
TGAGTATAGTGAGGAAGACG+AGG 0.671624 3_2:-49789670 Msa0409270:CDS
CATGAAACAAGATTCATCTG+TGG 0.677002 3_2:-49789751 Msa0409270:CDS
ATACCGCCAGCACAACGTGG+TGG 0.689012 3_2:+49789396 None:intergenic
ACATCATCGCTTAAGTCGAG+GGG 0.690101 3_2:+49789369 None:intergenic
TTCAAAGCAGCATTGCAGAG+GGG 0.703047 3_2:+49789330 None:intergenic
ACAATACCGCCAGCACAACG+TGG 0.758364 3_2:+49789393 None:intergenic
AAGCATAACCATAACCATGG+CGG 0.774143 3_2:+49788976 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCTTTAAAAGTAATGTAATA+TGG + chr3_2:49789554-49789573 None:intergenic 20.0%
!!! GCTTTAAAAGTAATGTAATA+TGG + chr3_2:49789554-49789573 None:intergenic 20.0%
!!! CACTTAATTTTGATTGATTC+TGG - chr3_2:49789214-49789233 Msa0409270:intron 25.0%
! ACTTTATCAAACACAAAAGT+TGG + chr3_2:49789485-49789504 None:intergenic 25.0%
! CATTAGTGACATAAATAACA+TGG + chr3_2:49789795-49789814 None:intergenic 25.0%
!! CACTAATGTTTCTGTATAAT+TGG - chr3_2:49789807-49789826 Msa0409270:CDS 25.0%
! AGAAAGAAATGAAACTCTAA+AGG + chr3_2:49789878-49789897 None:intergenic 25.0%
!!! CACTTAATTTTGATTGATTC+TGG - chr3_2:49789214-49789233 Msa0409270:intron 25.0%
! ACTTTATCAAACACAAAAGT+TGG + chr3_2:49789485-49789504 None:intergenic 25.0%
! CATTAGTGACATAAATAACA+TGG + chr3_2:49789795-49789814 None:intergenic 25.0%
!! CACTAATGTTTCTGTATAAT+TGG - chr3_2:49789807-49789826 Msa0409270:CDS 25.0%
! AGAAAGAAATGAAACTCTAA+AGG + chr3_2:49789878-49789897 None:intergenic 25.0%
!!! GATTTCGACATTTTGAAACA+CGG + chr3_2:49788804-49788823 None:intergenic 30.0%
GTGTTTCAAAATGTCGAAAT+CGG - chr3_2:49788803-49788822 Msa0409270:three_prime_UTR 30.0%
TGGTTCTTTGTATACGAATA+AGG - chr3_2:49789076-49789095 Msa0409270:CDS 30.0%
CAATCAAACGAATATTAAGC+AGG - chr3_2:49789367-49789386 Msa0409270:CDS 30.0%
AAAGTAATGTAATATGGCCT+AGG + chr3_2:49789548-49789567 None:intergenic 30.0%
!!! GATTTCGACATTTTGAAACA+CGG + chr3_2:49788804-49788823 None:intergenic 30.0%
GTGTTTCAAAATGTCGAAAT+CGG - chr3_2:49788803-49788822 Msa0409270:three_prime_UTR 30.0%
TGGTTCTTTGTATACGAATA+AGG - chr3_2:49789076-49789095 Msa0409270:CDS 30.0%
CAATCAAACGAATATTAAGC+AGG - chr3_2:49789367-49789386 Msa0409270:CDS 30.0%
AAAGTAATGTAATATGGCCT+AGG + chr3_2:49789548-49789567 None:intergenic 30.0%
!!! ATTTTGAAACACGGAAGGAA+TGG + chr3_2:49788795-49788814 None:intergenic 35.0%
ACACGATGAAGATTCATATG+AGG - chr3_2:49788872-49788891 Msa0409270:three_prime_UTR 35.0%
TGAAGATTCATATGAGGATG+AGG - chr3_2:49788878-49788897 Msa0409270:three_prime_UTR 35.0%
CATGAAACAAGATTCATCTG+TGG - chr3_2:49788917-49788936 Msa0409270:three_prime_UTR 35.0%
TCACTATACTCAGAATCTTC+TGG + chr3_2:49788990-49789009 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG - chr3_2:49788989-49789008 Msa0409270:exon 35.0%
! ACAATGACAGCTTTCCATTT+TGG - chr3_2:49789039-49789058 Msa0409270:CDS 35.0%
CTTTGTATACGAATAAGGCT+TGG - chr3_2:49789081-49789100 Msa0409270:CDS 35.0%
CAAACGAATATTAAGCAGGT+TGG - chr3_2:49789371-49789390 Msa0409270:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG - chr3_2:49789528-49789547 Msa0409270:intron 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG - chr3_2:49789568-49789587 Msa0409270:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG - chr3_2:49789569-49789588 Msa0409270:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG + chr3_2:49789653-49789672 None:intergenic 35.0%
AACAAGCATAACCATAACCA+TGG + chr3_2:49789698-49789717 None:intergenic 35.0%
! GGTTATGGTTATGCTTGTTT+TGG - chr3_2:49789699-49789718 Msa0409270:CDS 35.0%
!!! ATTTTGAAACACGGAAGGAA+TGG + chr3_2:49788795-49788814 None:intergenic 35.0%
ACACGATGAAGATTCATATG+AGG - chr3_2:49788872-49788891 Msa0409270:three_prime_UTR 35.0%
TGAAGATTCATATGAGGATG+AGG - chr3_2:49788878-49788897 Msa0409270:three_prime_UTR 35.0%
CATGAAACAAGATTCATCTG+TGG - chr3_2:49788917-49788936 Msa0409270:three_prime_UTR 35.0%
TCACTATACTCAGAATCTTC+TGG + chr3_2:49788990-49789009 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG - chr3_2:49788989-49789008 Msa0409270:exon 35.0%
! ACAATGACAGCTTTCCATTT+TGG - chr3_2:49789039-49789058 Msa0409270:CDS 35.0%
CTTTGTATACGAATAAGGCT+TGG - chr3_2:49789081-49789100 Msa0409270:CDS 35.0%
CAAACGAATATTAAGCAGGT+TGG - chr3_2:49789371-49789390 Msa0409270:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG - chr3_2:49789528-49789547 Msa0409270:intron 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG - chr3_2:49789568-49789587 Msa0409270:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG - chr3_2:49789569-49789588 Msa0409270:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG + chr3_2:49789653-49789672 None:intergenic 35.0%
AACAAGCATAACCATAACCA+TGG + chr3_2:49789698-49789717 None:intergenic 35.0%
! GGTTATGGTTATGCTTGTTT+TGG - chr3_2:49789699-49789718 Msa0409270:CDS 35.0%
!!! TCGACATTTTGAAACACGGA+AGG + chr3_2:49788800-49788819 None:intergenic 40.0%
CAAAATGTCGAAATCGGAAC+AGG - chr3_2:49788809-49788828 Msa0409270:three_prime_UTR 40.0%
AACAGGAACCGAAATCGAAA+CGG - chr3_2:49788826-49788845 Msa0409270:three_prime_UTR 40.0%
GATTTCAGCCGTTTCGATTT+CGG + chr3_2:49788837-49788856 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG - chr3_2:49788836-49788855 Msa0409270:three_prime_UTR 40.0%
AAATCGAAACGGCTGAAATC+GGG - chr3_2:49788837-49788856 Msa0409270:three_prime_UTR 40.0%
AGATTCATATGAGGATGAGG+AGG - chr3_2:49788881-49788900 Msa0409270:three_prime_UTR 40.0%
GAAACAAGATTCATCTGTGG+AGG - chr3_2:49788920-49788939 Msa0409270:three_prime_UTR 40.0%
TACTCAGAATCTTCTGGATC+CGG + chr3_2:49788984-49789003 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG + chr3_2:49789056-49789075 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG - chr3_2:49789056-49789075 Msa0409270:CDS 40.0%
TGTATACGAATAAGGCTTGG+AGG - chr3_2:49789084-49789103 Msa0409270:CDS 40.0%
GTATACGAATAAGGCTTGGA+GGG - chr3_2:49789085-49789104 Msa0409270:CDS 40.0%
GATTCTGGACAATACAAAGC+AGG - chr3_2:49789229-49789248 Msa0409270:intron 40.0%
TAAAGAAGTAGCACCAGGAT+GGG + chr3_2:49789519-49789538 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG + chr3_2:49789520-49789539 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG + chr3_2:49789524-49789543 None:intergenic 40.0%
AAATCTAATACACACCGCCA+TGG - chr3_2:49789678-49789697 Msa0409270:CDS 40.0%
AAGCATAACCATAACCATGG+CGG + chr3_2:49789695-49789714 None:intergenic 40.0%
!!! ATGCTTGTTTTGGTTTGCCT+TGG - chr3_2:49789709-49789728 Msa0409270:CDS 40.0%
!!! TCGACATTTTGAAACACGGA+AGG + chr3_2:49788800-49788819 None:intergenic 40.0%
CAAAATGTCGAAATCGGAAC+AGG - chr3_2:49788809-49788828 Msa0409270:three_prime_UTR 40.0%
AACAGGAACCGAAATCGAAA+CGG - chr3_2:49788826-49788845 Msa0409270:three_prime_UTR 40.0%
GATTTCAGCCGTTTCGATTT+CGG + chr3_2:49788837-49788856 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG - chr3_2:49788836-49788855 Msa0409270:three_prime_UTR 40.0%
AAATCGAAACGGCTGAAATC+GGG - chr3_2:49788837-49788856 Msa0409270:three_prime_UTR 40.0%
AGATTCATATGAGGATGAGG+AGG - chr3_2:49788881-49788900 Msa0409270:three_prime_UTR 40.0%
GAAACAAGATTCATCTGTGG+AGG - chr3_2:49788920-49788939 Msa0409270:three_prime_UTR 40.0%
TACTCAGAATCTTCTGGATC+CGG + chr3_2:49788984-49789003 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG + chr3_2:49789056-49789075 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG - chr3_2:49789056-49789075 Msa0409270:CDS 40.0%
TGTATACGAATAAGGCTTGG+AGG - chr3_2:49789084-49789103 Msa0409270:CDS 40.0%
GTATACGAATAAGGCTTGGA+GGG - chr3_2:49789085-49789104 Msa0409270:CDS 40.0%
GATTCTGGACAATACAAAGC+AGG - chr3_2:49789229-49789248 Msa0409270:intron 40.0%
TAAAGAAGTAGCACCAGGAT+GGG + chr3_2:49789519-49789538 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG + chr3_2:49789520-49789539 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG + chr3_2:49789524-49789543 None:intergenic 40.0%
AAATCTAATACACACCGCCA+TGG - chr3_2:49789678-49789697 Msa0409270:CDS 40.0%
AAGCATAACCATAACCATGG+CGG + chr3_2:49789695-49789714 None:intergenic 40.0%
!!! ATGCTTGTTTTGGTTTGCCT+TGG - chr3_2:49789709-49789728 Msa0409270:CDS 40.0%
ACAAGATTCATCTGTGGAGG+AGG - chr3_2:49788923-49788942 Msa0409270:three_prime_UTR 45.0%
GAATCTTCTGGATCCGGTTT+CGG + chr3_2:49788978-49788997 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG - chr3_2:49788998-49789017 Msa0409270:exon 45.0%
CGCTCTAGCAAACTAAGTGT+AGG - chr3_2:49789140-49789159 Msa0409270:CDS 45.0%
ACATCATCGCTTAAGTCGAG+GGG + chr3_2:49789302-49789321 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG + chr3_2:49789303-49789322 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG + chr3_2:49789341-49789360 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG + chr3_2:49789342-49789361 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG + chr3_2:49789343-49789362 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG + chr3_2:49789602-49789621 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG + chr3_2:49789619-49789638 None:intergenic 45.0%
AATACACACCGCCATGGTTA+TGG - chr3_2:49789684-49789703 Msa0409270:CDS 45.0%
ACTACACGTAAGTCTTGCCA+AGG + chr3_2:49789729-49789748 None:intergenic 45.0%
ACAAGATTCATCTGTGGAGG+AGG - chr3_2:49788923-49788942 Msa0409270:three_prime_UTR 45.0%
GAATCTTCTGGATCCGGTTT+CGG + chr3_2:49788978-49788997 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG - chr3_2:49788998-49789017 Msa0409270:exon 45.0%
CGCTCTAGCAAACTAAGTGT+AGG - chr3_2:49789140-49789159 Msa0409270:CDS 45.0%
ACATCATCGCTTAAGTCGAG+GGG + chr3_2:49789302-49789321 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG + chr3_2:49789303-49789322 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG + chr3_2:49789341-49789360 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG + chr3_2:49789342-49789361 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG + chr3_2:49789343-49789362 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG + chr3_2:49789602-49789621 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG + chr3_2:49789619-49789638 None:intergenic 45.0%
AATACACACCGCCATGGTTA+TGG - chr3_2:49789684-49789703 Msa0409270:CDS 45.0%
ACTACACGTAAGTCTTGCCA+AGG + chr3_2:49789729-49789748 None:intergenic 45.0%
!! TTACTTATTTATTATTAATT+AGG - chr3_2:49789457-49789476 Msa0409270:intron 5.0%
!! TACTTATTTATTATTAATTA+GGG - chr3_2:49789458-49789477 Msa0409270:intron 5.0%
!! AATTAATTAAATAAATATGA+TGG - chr3_2:49789750-49789769 Msa0409270:CDS 5.0%
!! ATTAATTAAATAAATATGAT+GGG - chr3_2:49789751-49789770 Msa0409270:CDS 5.0%
!! TTACTTATTTATTATTAATT+AGG - chr3_2:49789457-49789476 Msa0409270:intron 5.0%
!! TACTTATTTATTATTAATTA+GGG - chr3_2:49789458-49789477 Msa0409270:intron 5.0%
!! AATTAATTAAATAAATATGA+TGG - chr3_2:49789750-49789769 Msa0409270:CDS 5.0%
!! ATTAATTAAATAAATATGAT+GGG - chr3_2:49789751-49789770 Msa0409270:CDS 5.0%
GAAACGGCTGAAATCGGGAT+CGG - chr3_2:49788842-49788861 Msa0409270:three_prime_UTR 50.0%
TGAGGATGAGGAGGATAAGC+AGG - chr3_2:49788890-49788909 Msa0409270:three_prime_UTR 50.0%
AGATTCATCTGTGGAGGAGG+AGG - chr3_2:49788926-49788945 Msa0409270:three_prime_UTR 50.0%
GGAGGAGAAACGGCTGAAAT+CGG - chr3_2:49788950-49788969 Msa0409270:three_prime_UTR 50.0%
GCTGAAATCGGAACCGAAAC+CGG - chr3_2:49788962-49788981 Msa0409270:three_prime_UTR 50.0%
GCACATCATCGCTTAAGTCG+AGG + chr3_2:49789304-49789323 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG + chr3_2:49789328-49789347 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG - chr3_2:49789503-49789522 Msa0409270:intron 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG - chr3_2:49789572-49789591 Msa0409270:CDS 50.0%
GAAACGGCTGAAATCGGGAT+CGG - chr3_2:49788842-49788861 Msa0409270:three_prime_UTR 50.0%
TGAGGATGAGGAGGATAAGC+AGG - chr3_2:49788890-49788909 Msa0409270:three_prime_UTR 50.0%
AGATTCATCTGTGGAGGAGG+AGG - chr3_2:49788926-49788945 Msa0409270:three_prime_UTR 50.0%
GGAGGAGAAACGGCTGAAAT+CGG - chr3_2:49788950-49788969 Msa0409270:three_prime_UTR 50.0%
GCTGAAATCGGAACCGAAAC+CGG - chr3_2:49788962-49788981 Msa0409270:three_prime_UTR 50.0%
GCACATCATCGCTTAAGTCG+AGG + chr3_2:49789304-49789323 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG + chr3_2:49789328-49789347 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG - chr3_2:49789503-49789522 Msa0409270:intron 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG - chr3_2:49789572-49789591 Msa0409270:CDS 50.0%
TTCATCTGTGGAGGAGGAGG+AGG - chr3_2:49788929-49788948 Msa0409270:three_prime_UTR 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG - chr3_2:49788940-49788959 Msa0409270:three_prime_UTR 55.0%
TAAGGCTTGGAGGGATCGTC+AGG - chr3_2:49789094-49789113 Msa0409270:CDS 55.0%
ACAATACCGCCAGCACAACG+TGG + chr3_2:49789278-49789297 None:intergenic 55.0%
TTCATCTGTGGAGGAGGAGG+AGG - chr3_2:49788929-49788948 Msa0409270:three_prime_UTR 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG - chr3_2:49788940-49788959 Msa0409270:three_prime_UTR 55.0%
TAAGGCTTGGAGGGATCGTC+AGG - chr3_2:49789094-49789113 Msa0409270:CDS 55.0%
ACAATACCGCCAGCACAACG+TGG + chr3_2:49789278-49789297 None:intergenic 55.0%
ATCTGTGGAGGAGGAGGAGG+AGG - chr3_2:49788932-49788951 Msa0409270:three_prime_UTR 60.0%
!! CAGCACAACGTGGTGGACAG+TGG + chr3_2:49789268-49789287 None:intergenic 60.0%
CACTGTCCACCACGTTGTGC+TGG - chr3_2:49789266-49789285 Msa0409270:intron 60.0%
TGTCCACCACGTTGTGCTGG+CGG - chr3_2:49789269-49789288 Msa0409270:intron 60.0%
ATACCGCCAGCACAACGTGG+TGG + chr3_2:49789275-49789294 None:intergenic 60.0%
ATCTGTGGAGGAGGAGGAGG+AGG - chr3_2:49788932-49788951 Msa0409270:three_prime_UTR 60.0%
!! CAGCACAACGTGGTGGACAG+TGG + chr3_2:49789268-49789287 None:intergenic 60.0%
CACTGTCCACCACGTTGTGC+TGG - chr3_2:49789266-49789285 Msa0409270:intron 60.0%
TGTCCACCACGTTGTGCTGG+CGG - chr3_2:49789269-49789288 Msa0409270:intron 60.0%
ATACCGCCAGCACAACGTGG+TGG + chr3_2:49789275-49789294 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 49788790 49789900 49788790 ID=Msa0409270;Name=Msa0409270
chr3_2 mRNA 49788790 49789900 49788790 ID=Msa0409270-mRNA-1;Parent=Msa0409270;Name=Msa0409270-mRNA-1;_AED=0.36;_eAED=0.36;_QI=23|1|1|1|1|1|3|215|229
chr3_2 exon 49788790 49789211 49788790 ID=Msa0409270-mRNA-1:exon:9835;Parent=Msa0409270-mRNA-1
chr3_2 exon 49789302 49789439 49789302 ID=Msa0409270-mRNA-1:exon:9834;Parent=Msa0409270-mRNA-1
chr3_2 exon 49789533 49789900 49789533 ID=Msa0409270-mRNA-1:exon:9833;Parent=Msa0409270-mRNA-1
chr3_2 five_prime_UTR 49789878 49789900 49789878 ID=Msa0409270-mRNA-1:five_prime_utr;Parent=Msa0409270-mRNA-1
chr3_2 CDS 49789533 49789877 49789533 ID=Msa0409270-mRNA-1:cds;Parent=Msa0409270-mRNA-1
chr3_2 CDS 49789302 49789439 49789302 ID=Msa0409270-mRNA-1:cds;Parent=Msa0409270-mRNA-1
chr3_2 CDS 49789005 49789211 49789005 ID=Msa0409270-mRNA-1:cds;Parent=Msa0409270-mRNA-1
chr3_2 three_prime_UTR 49788790 49789004 49788790 ID=Msa0409270-mRNA-1:three_prime_utr;Parent=Msa0409270-mRNA-1
Gene Sequence

>Msa0409270

ATGTCGAAATCGGAACAGGAACCGAAATCGAAACGGCTGAAATCGGGATCGGAACAGAAACACGATGAAGATTCATATGAGGATGAGGAGGATAAGCAGGAATTCATGAAACAAGATTCATCTGTGGAGGAGGAGGAGGAGGAGAAACGGCTGAAATCGGAACCGAAACCGGATCCAGAAGATTCTGAGTATAGTGAGGAAGACGAGGATAACTTGTCTGTGCATGACAATGACAGCTTTCCATTTTGGTATAAGCGTCGTGATGGTTCTTTGTATACGAATAAGGCTTGGAGGGATCGTCAGGCTAAAATTCTTGACTATAAAAATCGCTCTAGCAAACTAAGTGTCTACGATGCTATCCACTGTCCACCACGTTGTGCTGGCGGTATTGTACCCCTCGACTTAAGCGATGATGTGCTCCGTACTCTCAAACCCCTCTGCAATGCTGCTTTGAAGATTTACAATCAAACGAATATTAAGCAGGGTCCAACTTTTGTGTTTGATAAAGTTGTCAAGTGTACCCATCCTGGTGCTACTTCTTTATATGCTCCTAGGCCATATTACATTACTTTTAAAGCAAAAGCAGAAGCAAAGGGAGGAAGACCTGCTCTAACAACTTTCCAGACTCATGTATTGCAGCGTAAAGATAAAGATCCTGTTGTCAAAGAGTGTTTCATTAAAAAAATCTAA
Protein sequence

>Msa0409270

MSKSEQEPKSKRLKSGSEQKHDEDSYEDEEDKQEFMKQDSSVEEEEEEKRLKSEPKPDPEDSEYSEEDEDNLSVHDNDSFPFWYKRRDGSLYTNKAWRDRQAKILDYKNRSSKLSVYDAIHCPPRCAGGIVPLDLSDDVLRTLKPLCNAALKIYNQTNIKQGPTFVFDKVVKCTHPGATSLYAPRPYYITFKAKAEAKGGRPALTTFQTHVLQRKDKDPVVKECFIKKI*