AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0506950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506950 MtrunA17_Chr3g0110271 96.028 428 14 3 1 425 664 1091 0.0 846
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0506950 AT5G37630.1 51.378 399 186 5 1 398 636 1027 1.67e-121 378

Find 97 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTCAAATTCATCAATTA+TGG 0.086387 3_4:+53809115 None:intergenic
TCTTGGTCTCTTTGGGCTTT+TGG 0.122377 3_4:-53812196 Msa0506950:five_prime_UTR
TCATCTTCATTGCTAGTTAA+AGG 0.161903 3_4:+53811889 None:intergenic
AGATGAAGTTAATCTCATTT+TGG 0.176342 3_4:-53809768 Msa0506950:CDS
GCTAAGGACTTCATACATTA+AGG 0.212444 3_4:-53812142 Msa0506950:five_prime_UTR
CGCATCTGAGACACCATAAA+AGG 0.234340 3_4:+53811383 None:intergenic
TCAGTCTCTTTAACGAATAA+TGG 0.234601 3_4:+53811311 None:intergenic
GTAAGATGTCTTGGTCTCTT+TGG 0.251500 3_4:-53812204 Msa0506950:five_prime_UTR
TTGTGGATGGTTTACCTCTA+TGG 0.265051 3_4:+53811187 None:intergenic
TAAGATGTCTTGGTCTCTTT+GGG 0.269846 3_4:-53812203 Msa0506950:five_prime_UTR
ACTCGACTTCAACCTGGATT+TGG 0.293852 3_4:-53809351 Msa0506950:CDS
ATCAATTATGGGTGATCTAA+TGG 0.324078 3_4:+53809127 None:intergenic
GCCACTGTTAATACGGTAAA+AGG 0.325788 3_4:-53809200 Msa0506950:CDS
CATTTGCTTGTGCAAGTCAT+AGG 0.329583 3_4:-53808907 Msa0506950:three_prime_UTR
TCTTCAAATTCATCAATTAT+GGG 0.341216 3_4:+53809116 None:intergenic
CTGTGACTCCGTTGGCCATT+TGG 0.349365 3_4:-53808819 Msa0506950:three_prime_UTR
GAATGCATTTATGCTGTTCT+TGG 0.350176 3_4:-53811868 Msa0506950:CDS
TATTGTTTGATTTAGATTGC+AGG 0.372737 3_4:-53808963 Msa0506950:three_prime_UTR
TTGTCACAGTTTACTTGGCT+TGG 0.400224 3_4:+53812021 None:intergenic
ATTTCTTGTCACAGTTTACT+TGG 0.401403 3_4:+53812016 None:intergenic
TGTTTCAAAAGTTCAGCATT+TGG 0.403143 3_4:+53812165 None:intergenic
AATGCATTTATGCTGTTCTT+GGG 0.415543 3_4:-53811867 Msa0506950:CDS
GCCACATTTAATTAGTATAG+AGG 0.422640 3_4:-53812118 Msa0506950:five_prime_UTR
CTAAGGACTTCATACATTAA+GGG 0.428149 3_4:-53812141 Msa0506950:five_prime_UTR
TGCAAAGAAGCCGGTATGCT+TGG 0.444374 3_4:+53811805 None:intergenic
GGCAGACGACATCTTGTTCA+TGG 0.457240 3_4:+53809148 None:intergenic
GGCTGTACAGCTGGTGTTTG+TGG 0.458710 3_4:+53809311 None:intergenic
AGAATTGCTGTAAGATGTCT+TGG 0.461269 3_4:-53812213 Msa0506950:five_prime_UTR
GGCCACATTGAACGCATAGC+TGG 0.462649 3_4:+53811434 None:intergenic
GCTCTCGTTGGCTGTACAGC+TGG 0.470915 3_4:+53809302 None:intergenic
ATTTATGCTGTTCTTGGGGA+AGG 0.473319 3_4:-53811862 Msa0506950:CDS
CCCTCCTCACCACACTCAAA+TGG 0.476517 3_4:+53811227 None:intergenic
CCATTTGAGTGTGGTGAGGA+GGG 0.476983 3_4:-53811227 Msa0506950:CDS
GATTCCCGCCACTGTTAATA+CGG 0.483662 3_4:-53809207 Msa0506950:CDS
GCAATATTGTCTATAACTTG+TGG 0.484729 3_4:+53811257 None:intergenic
TGATGAATTTGAAGAGCAAG+AGG 0.486928 3_4:-53809108 Msa0506950:CDS
AGCTATATATTTAACTAGAC+TGG 0.489337 3_4:+53809039 None:intergenic
CAATATTGTCTATAACTTGT+GGG 0.492294 3_4:+53811258 None:intergenic
TGAATGTGTATCTTCAGAAA+AGG 0.497037 3_4:-53809867 Msa0506950:CDS
CATTTGAGTGTGGTGAGGAG+GGG 0.497051 3_4:-53811226 Msa0506950:CDS
CTGATACATCACTGAAGTAC+AGG 0.498479 3_4:+53811755 None:intergenic
GCATCTGAGACACCATAAAA+GGG 0.500033 3_4:+53811384 None:intergenic
GTGGCTTTGAAAATGAAGAC+TGG 0.506420 3_4:-53811918 Msa0506950:CDS
CAACAGAAGCATCCAAATCC+AGG 0.510663 3_4:+53809339 None:intergenic
ATCATCTAATGACAATTGAC+AGG 0.511830 3_4:-53809814 Msa0506950:CDS
TCCATTTGAGTGTGGTGAGG+AGG 0.514524 3_4:-53811228 Msa0506950:CDS
AAAACCTTGTCAACTTCTTG+AGG 0.515022 3_4:+53812057 None:intergenic
GAGCCACACGTGCAAGGAGA+AGG 0.517188 3_4:-53809283 Msa0506950:CDS
GAAGAAGACAGCTGCTGAGA+AGG 0.523042 3_4:-53809990 Msa0506950:CDS
CTTGCACGTGTGGCTCTCGT+TGG 0.523140 3_4:+53809290 None:intergenic
AACTTGCTCTCAATACCAAA+TGG 0.526173 3_4:+53808804 None:intergenic
TTCACGATTTATGTTGCAGA+TGG 0.529130 3_4:-53811342 Msa0506950:CDS
TACCCTTCTCCTTGCACGTG+TGG 0.533430 3_4:+53809280 None:intergenic
CGCACTACGCATAGCCATAG+AGG 0.534242 3_4:-53811201 Msa0506950:intron
TGCTTGGATAGTTATCGCTT+AGG 0.535282 3_4:+53811821 None:intergenic
AGCACAGGGTGCAAAGAAGC+CGG 0.535677 3_4:+53811796 None:intergenic
ATGAGCTTAGATAAAAGCAC+AGG 0.535927 3_4:+53811781 None:intergenic
AACACAGAGGTGTATATTGA+AGG 0.537558 3_4:-53811465 Msa0506950:intron
CATTTAATTAGTATAGAGGC+TGG 0.537733 3_4:-53812114 Msa0506950:five_prime_UTR
CAAATGGCCAACGGAGTCAC+AGG 0.540398 3_4:+53808820 None:intergenic
TGTTAAAGAATTGAAACGCA+TGG 0.546500 3_4:-53809840 Msa0506950:CDS
GTGAACTTCTCTGATTCTGA+AGG 0.548776 3_4:-53811982 Msa0506950:CDS
TTGTGATTTGTTGAGAGACA+TGG 0.551010 3_4:+53811601 None:intergenic
CACCATAAAAGGGGAACCCC+CGG 0.553359 3_4:+53811394 None:intergenic
ACTGGAACTCGACTTCAACC+TGG 0.556588 3_4:-53809357 Msa0506950:CDS
CGATAACTATCCAAGCATAC+CGG 0.558116 3_4:-53811815 Msa0506950:CDS
GCTATGCGTTCAATGTGGCC+AGG 0.567131 3_4:-53811431 Msa0506950:CDS
CAACGAGAGCCACACGTGCA+AGG 0.571146 3_4:-53809289 Msa0506950:CDS
TGAACTTCTCTGATTCTGAA+GGG 0.576518 3_4:-53811981 Msa0506950:CDS
GAGGAAACTTCTATGTCGTA+TGG 0.579263 3_4:-53809891 Msa0506950:CDS
ATTATTACCTGTGACTCCGT+TGG 0.586873 3_4:-53808827 Msa0506950:three_prime_UTR
GAACTTCTCTGATTCTGAAG+GGG 0.588638 3_4:-53811980 Msa0506950:CDS
TCAGAATCAGAGAAGTTCAC+AGG 0.594318 3_4:+53811985 None:intergenic
CGGTCCTCAAGAAGTTGACA+AGG 0.596046 3_4:-53812061 Msa0506950:CDS
TTTACCGTATTAACAGTGGC+GGG 0.596262 3_4:+53809203 None:intergenic
TAAGGACTTCATACATTAAG+GGG 0.602541 3_4:-53812140 Msa0506950:five_prime_UTR
AGCCACACGTGCAAGGAGAA+GGG 0.602740 3_4:-53809282 Msa0506950:CDS
TGGCTTTGAAAATGAAGACT+GGG 0.604797 3_4:-53811917 Msa0506950:CDS
GTCTCAGATGCGTAAGCGTG+CGG 0.606283 3_4:-53811372 Msa0506950:CDS
GCCTCTATACTAATTAAATG+TGG 0.613343 3_4:+53812117 None:intergenic
ATGTATCAGAAAATATGCAC+AGG 0.613792 3_4:-53811741 Msa0506950:intron
AGTTAAATATATAGCTAACA+TGG 0.618068 3_4:-53809032 Msa0506950:exon
ATATATTTAACTAGACTGGT+CGG 0.625813 3_4:+53809043 None:intergenic
AAGGAGAAGGGTAAGGATCG+AGG 0.636337 3_4:-53809270 Msa0506950:CDS
CACGTGCAAGGAGAAGGGTA+AGG 0.639412 3_4:-53809277 Msa0506950:CDS
TGTGATTTGTTGAGAGACAT+GGG 0.640214 3_4:+53811602 None:intergenic
ATTGATCTTGTGATGTGGCA+CGG 0.642720 3_4:-53812081 Msa0506950:exon
ATGCATTTATGCTGTTCTTG+GGG 0.658448 3_4:-53811866 Msa0506950:CDS
GCTGAAGTTCCATTTGAGTG+TGG 0.658594 3_4:-53811236 Msa0506950:CDS
CATTAATTGATCTTGTGATG+TGG 0.666350 3_4:-53812086 Msa0506950:exon
ATGTCTCTCAACAAATCACA+AGG 0.670858 3_4:-53811599 Msa0506950:intron
CATCTGAGACACCATAAAAG+GGG 0.671973 3_4:+53811385 None:intergenic
TGAGCTTAGATAAAAGCACA+GGG 0.674749 3_4:+53811782 None:intergenic
TCTCAATACCAAATGGCCAA+CGG 0.678875 3_4:+53808811 None:intergenic
TTCCAGCTATGCGTTCAATG+TGG 0.679058 3_4:-53811436 Msa0506950:CDS
GAAGCAGTGAGTGAAAACTC+TGG 0.680430 3_4:-53811290 Msa0506950:CDS
AGTTCCATTTGAGTGTGGTG+AGG 0.698415 3_4:-53811231 Msa0506950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTACGAAATAAAACAATTA+AGG + chr3_4:53809489-53809508 None:intergenic 15.0%
!!! GTTAACTTATTTTTTTTTTG+TGG - chr3_4:53810030-53810049 Msa0506950:intron 15.0%
!!! TAATTTTAACTATAAGAATC+TGG + chr3_4:53810556-53810575 None:intergenic 15.0%
!!! ATCTAATTTTTAAATTCAGT+TGG + chr3_4:53811356-53811375 None:intergenic 15.0%
!! AGAAAAATAAGTAAAAACGA+TGG + chr3_4:53809440-53809459 None:intergenic 20.0%
!! TAAATGATATCTGCATATTA+TGG - chr3_4:53809965-53809984 Msa0506950:CDS 20.0%
!! AATATCTTAATGAATTGAAC+AGG - chr3_4:53810206-53810225 Msa0506950:intron 20.0%
!! ATATCTTAATGAATTGAACA+GGG - chr3_4:53810207-53810226 Msa0506950:intron 20.0%
!! TTTCCTTCAAAAAAATAAGA+TGG + chr3_4:53811595-53811614 None:intergenic 20.0%
!!! TTATTTTTTTGAAGGAAAAC+TGG - chr3_4:53811597-53811616 Msa0506950:intron 20.0%
!! TCTTCAAATTCATCAATTAT+GGG + chr3_4:53811859-53811878 None:intergenic 20.0%
!! AGTTAAATATATAGCTAACA+TGG - chr3_4:53811940-53811959 Msa0506950:CDS 20.0%
!! GACATTCTTGATCTTTTATA+CGG - chr3_4:53809032-53809051 Msa0506950:exon 25.0%
! CTAAATTAGCTAATTGTACA+GGG + chr3_4:53809291-53809310 None:intergenic 25.0%
! TCTAAATTAGCTAATTGTAC+AGG + chr3_4:53809292-53809311 None:intergenic 25.0%
!! CAATATTGTCTATAACTTGT+GGG + chr3_4:53809717-53809736 None:intergenic 25.0%
!! TTTAACAAACTTCAAGTTTG+TGG + chr3_4:53809805-53809824 None:intergenic 25.0%
! TCAATTAGAACAGAATTATG+TGG + chr3_4:53809924-53809943 None:intergenic 25.0%
!! TCTGTTCTAATTGATTCTAA+AGG - chr3_4:53809930-53809949 Msa0506950:CDS 25.0%
!!! TATTTTTTTTTTGTGGTGTC+CGG - chr3_4:53810037-53810056 Msa0506950:intron 25.0%
! GATAATGCATAATATATGCA+AGG + chr3_4:53810078-53810097 None:intergenic 25.0%
!!! AGGGAAAGTTGATTTTTAAT+GGG - chr3_4:53810226-53810245 Msa0506950:intron 25.0%
!!! ACACATTTTAACACAGAAAA+AGG - chr3_4:53810312-53810331 Msa0506950:intron 25.0%
!!! CACATTTTAACACAGAAAAA+GGG - chr3_4:53810313-53810332 Msa0506950:intron 25.0%
! TATATCAACAGTAACATCAT+AGG + chr3_4:53810475-53810494 None:intergenic 25.0%
!!! ACCTTTTTGTTGTATATTCT+TGG - chr3_4:53810579-53810598 Msa0506950:intron 25.0%
! TCCAAGAATATACAACAAAA+AGG + chr3_4:53810583-53810602 None:intergenic 25.0%
! AAATTGAAAATAGTCTGAAC+TGG - chr3_4:53810706-53810725 Msa0506950:intron 25.0%
! AATTGAAAATAGTCTGAACT+GGG - chr3_4:53810707-53810726 Msa0506950:intron 25.0%
! TATATGAATTTCATGTCAGA+TGG - chr3_4:53810868-53810887 Msa0506950:intron 25.0%
! ATATGAATTTCATGTCAGAT+GGG - chr3_4:53810869-53810888 Msa0506950:intron 25.0%
!! AGTTTCCTCGTAATTTTTAT+TGG + chr3_4:53811070-53811089 None:intergenic 25.0%
!! AGATGAAGTTAATCTCATTT+TGG - chr3_4:53811204-53811223 Msa0506950:CDS 25.0%
!! GATGAAGTTAATCTCATTTT+GGG - chr3_4:53811205-53811224 Msa0506950:CDS 25.0%
! ATATTATTATGATTGATGCG+AGG - chr3_4:53811300-53811319 Msa0506950:CDS 25.0%
! GCTGTATTATTCAGTATTAT+TGG - chr3_4:53811520-53811539 Msa0506950:intron 25.0%
!!! CTACCATCTTATTTTTTTGA+AGG - chr3_4:53811589-53811608 Msa0506950:intron 25.0%
! CTCTTCAAATTCATCAATTA+TGG + chr3_4:53811860-53811879 None:intergenic 25.0%
! ATATATTTAACTAGACTGGT+CGG + chr3_4:53811932-53811951 None:intergenic 25.0%
! AGCTATATATTTAACTAGAC+TGG + chr3_4:53811936-53811955 None:intergenic 25.0%
!! TATTGTTTGATTTAGATTGC+AGG - chr3_4:53812009-53812028 Msa0506950:CDS 25.0%
TGTTTCAAAAGTTCAGCATT+TGG + chr3_4:53808810-53808829 None:intergenic 30.0%
CTAAGGACTTCATACATTAA+GGG - chr3_4:53808831-53808850 Msa0506950:three_prime_UTR 30.0%
TAAGGACTTCATACATTAAG+GGG - chr3_4:53808832-53808851 Msa0506950:three_prime_UTR 30.0%
GCCACATTTAATTAGTATAG+AGG - chr3_4:53808854-53808873 Msa0506950:three_prime_UTR 30.0%
GCCTCTATACTAATTAAATG+TGG + chr3_4:53808858-53808877 None:intergenic 30.0%
CATTTAATTAGTATAGAGGC+TGG - chr3_4:53808858-53808877 Msa0506950:three_prime_UTR 30.0%
CATTAATTGATCTTGTGATG+TGG - chr3_4:53808886-53808905 Msa0506950:three_prime_UTR 30.0%
ATTTCTTGTCACAGTTTACT+TGG + chr3_4:53808959-53808978 None:intergenic 30.0%
! ATTCTTGATCTTTTATACGG+TGG - chr3_4:53809035-53809054 Msa0506950:exon 30.0%
TCATCTTCATTGCTAGTTAA+AGG + chr3_4:53809086-53809105 None:intergenic 30.0%
AATGCATTTATGCTGTTCTT+GGG - chr3_4:53809105-53809124 Msa0506950:CDS 30.0%
ATGTATCAGAAAATATGCAC+AGG - chr3_4:53809231-53809250 Msa0506950:CDS 30.0%
CGAAATAAAACAATTAAGGC+AGG + chr3_4:53809485-53809504 None:intergenic 30.0%
!!! GTTTTATTTCGTAAACACAG+AGG - chr3_4:53809494-53809513 Msa0506950:intron 30.0%
TCAGTCTCTTTAACGAATAA+TGG + chr3_4:53809664-53809683 None:intergenic 30.0%
GCAATATTGTCTATAACTTG+TGG + chr3_4:53809718-53809737 None:intergenic 30.0%
TCACCTAACTTGATATTCAA+AGG - chr3_4:53809848-53809867 Msa0506950:CDS 30.0%
!!! ATTTTTTTTTTGTGGTGTCC+GGG - chr3_4:53810038-53810057 Msa0506950:intron 30.0%
!! CAGGGAAAGTTGATTTTTAA+TGG - chr3_4:53810225-53810244 Msa0506950:intron 30.0%
!! GGGAAAGTTGATTTTTAATG+GGG - chr3_4:53810227-53810246 Msa0506950:intron 30.0%
TTCTGTGTTAAAATGTGTAC+AGG + chr3_4:53810310-53810329 None:intergenic 30.0%
! ACATCATTTTGTGCAATGTT+TGG + chr3_4:53810398-53810417 None:intergenic 30.0%
! CACAAAATGATGTATTGATC+TGG - chr3_4:53810405-53810424 Msa0506950:intron 30.0%
ATAATTCAGTCCAAAATCCT+AGG + chr3_4:53810433-53810452 None:intergenic 30.0%
! TTTTGGACTGAATTATTGTG+CGG - chr3_4:53810437-53810456 Msa0506950:intron 30.0%
TTATCAGTAAATGCAACTAG+AGG - chr3_4:53810530-53810549 Msa0506950:intron 30.0%
AGCAGAATTAAGCAATATAG+TGG + chr3_4:53810777-53810796 None:intergenic 30.0%
ACTATATTGCTTAATTCTGC+TGG - chr3_4:53810776-53810795 Msa0506950:intron 30.0%
TCTTTCTCTTCACTGTATAA+CGG + chr3_4:53810841-53810860 None:intergenic 30.0%
TATGAATTTCATGTCAGATG+GGG - chr3_4:53810870-53810889 Msa0506950:intron 30.0%
! TTTTTCTTTCCTCTCTGTTA+AGG - chr3_4:53810943-53810962 Msa0506950:intron 30.0%
! TACTTGTCTCACTTCATTTT+CGG - chr3_4:53811029-53811048 Msa0506950:intron 30.0%
! ATTTTCGGTTATCAGAACAA+GGG - chr3_4:53811044-53811063 Msa0506950:intron 30.0%
TGAATGTGTATCTTCAGAAA+AGG - chr3_4:53811105-53811124 Msa0506950:intron 30.0%
TGTTAAAGAATTGAAACGCA+TGG - chr3_4:53811132-53811151 Msa0506950:intron 30.0%
ATCATCTAATGACAATTGAC+AGG - chr3_4:53811158-53811177 Msa0506950:intron 30.0%
AGATAAATGTTAGCTGTAAG+AGG + chr3_4:53811568-53811587 None:intergenic 30.0%
ATCAATTATGGGTGATCTAA+TGG + chr3_4:53811848-53811867 None:intergenic 30.0%
AGAATTGCTGTAAGATGTCT+TGG - chr3_4:53808759-53808778 Msa0506950:three_prime_UTR 35.0%
TAAGATGTCTTGGTCTCTTT+GGG - chr3_4:53808769-53808788 Msa0506950:three_prime_UTR 35.0%
!!! CTGAACTTTTGAAACAGCTA+AGG - chr3_4:53808814-53808833 Msa0506950:three_prime_UTR 35.0%
GCTAAGGACTTCATACATTA+AGG - chr3_4:53808830-53808849 Msa0506950:three_prime_UTR 35.0%
AAAACCTTGTCAACTTCTTG+AGG + chr3_4:53808918-53808937 None:intergenic 35.0%
TGAACTTCTCTGATTCTGAA+GGG - chr3_4:53808991-53809010 Msa0506950:three_prime_UTR 35.0%
!! TGGCTTTGAAAATGAAGACT+GGG - chr3_4:53809055-53809074 Msa0506950:CDS 35.0%
GAATGCATTTATGCTGTTCT+TGG - chr3_4:53809104-53809123 Msa0506950:CDS 35.0%
ATGCATTTATGCTGTTCTTG+GGG - chr3_4:53809106-53809125 Msa0506950:CDS 35.0%
! TGAGCTTAGATAAAAGCACA+GGG + chr3_4:53809193-53809212 None:intergenic 35.0%
! ATGAGCTTAGATAAAAGCAC+AGG + chr3_4:53809194-53809213 None:intergenic 35.0%
TATCAGAAAATATGCACAGG+TGG - chr3_4:53809234-53809253 Msa0506950:CDS 35.0%
!! TGTGATTTGTTGAGAGACAT+GGG + chr3_4:53809373-53809392 None:intergenic 35.0%
!! TTGTGATTTGTTGAGAGACA+TGG + chr3_4:53809374-53809393 None:intergenic 35.0%
ATGTCTCTCAACAAATCACA+AGG - chr3_4:53809373-53809392 Msa0506950:intron 35.0%
! AACACAGAGGTGTATATTGA+AGG - chr3_4:53809507-53809526 Msa0506950:intron 35.0%
TTCACGATTTATGTTGCAGA+TGG - chr3_4:53809630-53809649 Msa0506950:intron 35.0%
! ACAAACTTCAAGTTTGTGGA+TGG + chr3_4:53809801-53809820 None:intergenic 35.0%
GTGCCTTTGAATATCAAGTT+AGG + chr3_4:53809854-53809873 None:intergenic 35.0%
ACCTTCACCTTAATATGGAT+TGG - chr3_4:53809876-53809895 Msa0506950:CDS 35.0%
ACCAATCCATATTAAGGTGA+AGG + chr3_4:53809880-53809899 None:intergenic 35.0%
CCAAGTACCAATCCATATTA+AGG + chr3_4:53809886-53809905 None:intergenic 35.0%
!! CCTTAATATGGATTGGTACT+TGG - chr3_4:53809883-53809902 Msa0506950:CDS 35.0%
!! AATATGGATTGGTACTTGGT+AGG - chr3_4:53809887-53809906 Msa0506950:CDS 35.0%
CATAATATATGCAAGGTCTG+AGG + chr3_4:53810071-53810090 None:intergenic 35.0%
ACAGGCAAAAGTTGTACTAA+CGG + chr3_4:53810292-53810311 None:intergenic 35.0%
ATTTCTTGCAGCATACGAAA+AGG + chr3_4:53810662-53810681 None:intergenic 35.0%
TTTCGTATGCTGCAAGAAAT+TGG - chr3_4:53810662-53810681 Msa0506950:intron 35.0%
! ATTGCGTACTTGTTGAAGTT+TGG - chr3_4:53810741-53810760 Msa0506950:intron 35.0%
CTTTCTCTTCACTGTATAAC+GGG + chr3_4:53810840-53810859 None:intergenic 35.0%
! CATTTTCGGTTATCAGAACA+AGG - chr3_4:53811043-53811062 Msa0506950:intron 35.0%
AAGGGCCAATAAAAATTACG+AGG - chr3_4:53811062-53811081 Msa0506950:intron 35.0%
ATGATTACATGCCTAGACTT+TGG - chr3_4:53811263-53811282 Msa0506950:CDS 35.0%
!!! ACACGAAGTATCACTTTTTC+AGG - chr3_4:53811381-53811400 Msa0506950:CDS 35.0%
! TGTTAGCTGTAAGAGGTTTT+CGG + chr3_4:53811561-53811580 None:intergenic 35.0%
! TTTTACCGTATTAACAGTGG+CGG + chr3_4:53811773-53811792 None:intergenic 35.0%
! ACCTTTTACCGTATTAACAG+TGG + chr3_4:53811776-53811795 None:intergenic 35.0%
TGATGAATTTGAAGAGCAAG+AGG - chr3_4:53811864-53811883 Msa0506950:CDS 35.0%
! AACTTGCTCTCAATACCAAA+TGG + chr3_4:53812171-53812190 None:intergenic 35.0%
GTAAGATGTCTTGGTCTCTT+TGG - chr3_4:53808768-53808787 Msa0506950:three_prime_UTR 40.0%
ATTGATCTTGTGATGTGGCA+CGG - chr3_4:53808891-53808910 Msa0506950:three_prime_UTR 40.0%
TTGTCACAGTTTACTTGGCT+TGG + chr3_4:53808954-53808973 None:intergenic 40.0%
TCAGAATCAGAGAAGTTCAC+AGG + chr3_4:53808990-53809009 None:intergenic 40.0%
GTGAACTTCTCTGATTCTGA+AGG - chr3_4:53808990-53809009 Msa0506950:three_prime_UTR 40.0%
! GAACTTCTCTGATTCTGAAG+GGG - chr3_4:53808992-53809011 Msa0506950:three_prime_UTR 40.0%
!! GTGGCTTTGAAAATGAAGAC+TGG - chr3_4:53809054-53809073 Msa0506950:CDS 40.0%
ATTTATGCTGTTCTTGGGGA+AGG - chr3_4:53809110-53809129 Msa0506950:CDS 40.0%
TGCTTGGATAGTTATCGCTT+AGG + chr3_4:53809154-53809173 None:intergenic 40.0%
CGATAACTATCCAAGCATAC+CGG - chr3_4:53809157-53809176 Msa0506950:CDS 40.0%
CTGATACATCACTGAAGTAC+AGG + chr3_4:53809220-53809239 None:intergenic 40.0%
CAGGGCAAGAATAGTACAAA+GGG + chr3_4:53809273-53809292 None:intergenic 40.0%
ACAGGGCAAGAATAGTACAA+AGG + chr3_4:53809274-53809293 None:intergenic 40.0%
! TCAAATGTTTTTCACCTGCC+AGG - chr3_4:53809312-53809331 Msa0506950:CDS 40.0%
! AGATAAGCATTGCTTCAACC+TGG + chr3_4:53809333-53809352 None:intergenic 40.0%
! TCAATGTGGCCAGGAATTTT+TGG - chr3_4:53809550-53809569 Msa0506950:intron 40.0%
!!! CCAGGAATTTTTGGCAATTC+CGG - chr3_4:53809559-53809578 Msa0506950:intron 40.0%
!!! CAGGAATTTTTGGCAATTCC+GGG - chr3_4:53809560-53809579 Msa0506950:intron 40.0%
!!! AGGAATTTTTGGCAATTCCG+GGG - chr3_4:53809561-53809580 Msa0506950:intron 40.0%
! CATCTGAGACACCATAAAAG+GGG + chr3_4:53809590-53809609 None:intergenic 40.0%
! GCATCTGAGACACCATAAAA+GGG + chr3_4:53809591-53809610 None:intergenic 40.0%
TTGTGGATGGTTTACCTCTA+TGG + chr3_4:53809788-53809807 None:intergenic 40.0%
CACACACCTTCACCTTAATA+TGG - chr3_4:53809871-53809890 Msa0506950:CDS 40.0%
! GATGTATTGATCTGGAACCT+AGG - chr3_4:53810413-53810432 Msa0506950:intron 40.0%
!! TGATCTGGAACCTAGGATTT+TGG - chr3_4:53810420-53810439 Msa0506950:intron 40.0%
TTCCCATATGTAACATGCAC+AGG + chr3_4:53810688-53810707 None:intergenic 40.0%
AAATAGTCTGAACTGGGTGT+TGG - chr3_4:53810713-53810732 Msa0506950:intron 40.0%
TAATTCTGCTGGATGTCACT+TGG - chr3_4:53810787-53810806 Msa0506950:intron 40.0%
GAGGAAACTTCTATGTCGTA+TGG - chr3_4:53811081-53811100 Msa0506950:intron 40.0%
CACAACAAGAACCAAAGTCT+AGG + chr3_4:53811277-53811296 None:intergenic 40.0%
!! CTAGACTTTGGTTCTTGTTG+TGG - chr3_4:53811275-53811294 Msa0506950:CDS 40.0%
! GTTAGCTGTAAGAGGTTTTC+GGG + chr3_4:53811560-53811579 None:intergenic 40.0%
TTTACCGTATTAACAGTGGC+GGG + chr3_4:53811772-53811791 None:intergenic 40.0%
GCCACTGTTAATACGGTAAA+AGG - chr3_4:53811772-53811791 Msa0506950:CDS 40.0%
CATTTGCTTGTGCAAGTCAT+AGG - chr3_4:53812065-53812084 Msa0506950:CDS 40.0%
ATTATTACCTGTGACTCCGT+TGG - chr3_4:53812145-53812164 Msa0506950:five_prime_UTR 40.0%
TCTCAATACCAAATGGCCAA+CGG + chr3_4:53812164-53812183 None:intergenic 40.0%
! TCTTGGTCTCTTTGGGCTTT+TGG - chr3_4:53808776-53808795 Msa0506950:three_prime_UTR 45.0%
TTCCAGCTATGCGTTCAATG+TGG - chr3_4:53809536-53809555 Msa0506950:intron 45.0%
CCGGAATTGCCAAAAATTCC+TGG + chr3_4:53809562-53809581 None:intergenic 45.0%
!!! GGAATTTTTGGCAATTCCGG+GGG - chr3_4:53809562-53809581 Msa0506950:intron 45.0%
! CGCATCTGAGACACCATAAA+AGG + chr3_4:53809592-53809611 None:intergenic 45.0%
GAAGCAGTGAGTGAAAACTC+TGG - chr3_4:53809682-53809701 Msa0506950:intron 45.0%
GCTGAAGTTCCATTTGAGTG+TGG - chr3_4:53809736-53809755 Msa0506950:intron 45.0%
AGTTCCATTTGAGTGTGGTG+AGG - chr3_4:53809741-53809760 Msa0506950:intron 45.0%
! TGAGCTTAGGTCAGTTGGTA+GGG + chr3_4:53810100-53810119 None:intergenic 45.0%
TACCAACTGACCTAAGCTCA+CGG - chr3_4:53810100-53810119 Msa0506950:intron 45.0%
GCATACGAAAAGGCTTCACT+AGG + chr3_4:53810652-53810671 None:intergenic 45.0%
TGGCCTGTGCATGTTACATA+TGG - chr3_4:53810682-53810701 Msa0506950:intron 45.0%
GGCCTGTGCATGTTACATAT+GGG - chr3_4:53810683-53810702 Msa0506950:intron 45.0%
ACTCGACTTCAACCTGGATT+TGG - chr3_4:53811621-53811640 Msa0506950:CDS 45.0%
!! CAACAGAAGCATCCAAATCC+AGG + chr3_4:53811636-53811655 None:intergenic 45.0%
GATTCCCGCCACTGTTAATA+CGG - chr3_4:53811765-53811784 Msa0506950:CDS 45.0%
!! GTCTCTTTGGGCTTTTGGAG+AGG - chr3_4:53808781-53808800 Msa0506950:three_prime_UTR 50.0%
CGGTCCTCAAGAAGTTGACA+AGG - chr3_4:53808911-53808930 Msa0506950:three_prime_UTR 50.0%
TGCAAAGAAGCCGGTATGCT+TGG + chr3_4:53809170-53809189 None:intergenic 50.0%
! AAGCATTGCTTCAACCTGGC+AGG + chr3_4:53809329-53809348 None:intergenic 50.0%
TCCATTTGAGTGTGGTGAGG+AGG - chr3_4:53809744-53809763 Msa0506950:intron 50.0%
CCATTTGAGTGTGGTGAGGA+GGG - chr3_4:53809745-53809764 Msa0506950:intron 50.0%
CATTTGAGTGTGGTGAGGAG+GGG - chr3_4:53809746-53809765 Msa0506950:intron 50.0%
! AAGGTCTGAGGTTCGAATCC+CGG + chr3_4:53810059-53810078 None:intergenic 50.0%
GTGAGCTTAGGTCAGTTGGT+AGG + chr3_4:53810101-53810120 None:intergenic 50.0%
ACCAACTGACCTAAGCTCAC+GGG - chr3_4:53810101-53810120 Msa0506950:intron 50.0%
AACTCTATCCCCGTGAGCTT+AGG + chr3_4:53810113-53810132 None:intergenic 50.0%
AGCTTGCGACCTTAACAGAG+AGG + chr3_4:53810955-53810974 None:intergenic 50.0%
GAAGAAGACAGCTGCTGAGA+AGG - chr3_4:53810982-53811001 Msa0506950:intron 50.0%
!! GCTGTAAGAGGTTTTCGGGA+GGG + chr3_4:53811556-53811575 None:intergenic 50.0%
!! AGCTGTAAGAGGTTTTCGGG+AGG + chr3_4:53811557-53811576 None:intergenic 50.0%
ACTGGAACTCGACTTCAACC+TGG - chr3_4:53811615-53811634 Msa0506950:CDS 50.0%
AAGGAGAAGGGTAAGGATCG+AGG - chr3_4:53811702-53811721 Msa0506950:intron 50.0%
GGCAGACGACATCTTGTTCA+TGG + chr3_4:53811827-53811846 None:intergenic 50.0%
AGCACAGGGTGCAAAGAAGC+CGG + chr3_4:53809179-53809198 None:intergenic 55.0%
GGCCACATTGAACGCATAGC+TGG + chr3_4:53809541-53809560 None:intergenic 55.0%
GCTATGCGTTCAATGTGGCC+AGG - chr3_4:53809541-53809560 Msa0506950:intron 55.0%
! TTCCGGGGGTTCCCCTTTTA+TGG - chr3_4:53809576-53809595 Msa0506950:intron 55.0%
CACCATAAAAGGGGAACCCC+CGG + chr3_4:53809581-53809600 None:intergenic 55.0%
GTCTCAGATGCGTAAGCGTG+CGG - chr3_4:53809600-53809619 Msa0506950:intron 55.0%
CCCTCCTCACCACACTCAAA+TGG + chr3_4:53809748-53809767 None:intergenic 55.0%
CGCACTACGCATAGCCATAG+AGG - chr3_4:53809771-53809790 Msa0506950:CDS 55.0%
CCAACTGACCTAAGCTCACG+GGG - chr3_4:53810102-53810121 Msa0506950:intron 55.0%
GGCTGTACAGCTGGTGTTTG+TGG + chr3_4:53811664-53811683 None:intergenic 55.0%
AGCCACACGTGCAAGGAGAA+GGG - chr3_4:53811690-53811709 Msa0506950:intron 55.0%
TACCCTTCTCCTTGCACGTG+TGG + chr3_4:53811695-53811714 None:intergenic 55.0%
CACGTGCAAGGAGAAGGGTA+AGG - chr3_4:53811695-53811714 Msa0506950:intron 55.0%
CAAATGGCCAACGGAGTCAC+AGG + chr3_4:53812155-53812174 None:intergenic 55.0%
CTGTGACTCCGTTGGCCATT+TGG - chr3_4:53812153-53812172 Msa0506950:five_prime_UTR 55.0%
CCCCGTGAGCTTAGGTCAGT+TGG + chr3_4:53810105-53810124 None:intergenic 60.0%
! GCTCTCGTTGGCTGTACAGC+TGG + chr3_4:53811673-53811692 None:intergenic 60.0%
CTTGCACGTGTGGCTCTCGT+TGG + chr3_4:53811685-53811704 None:intergenic 60.0%
CAACGAGAGCCACACGTGCA+AGG - chr3_4:53811683-53811702 Msa0506950:intron 60.0%
GAGCCACACGTGCAAGGAGA+AGG - chr3_4:53811689-53811708 Msa0506950:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 53808746 53812248 53808746 ID=Msa0506950;Name=Msa0506950
chr3_4 mRNA 53808746 53812248 53808746 ID=Msa0506950-mRNA-1;Parent=Msa0506950;Name=Msa0506950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=157|1|1|1|0.5|0.6|5|303|428
chr3_4 exon 53808746 53809383 53808746 ID=Msa0506950-mRNA-1:exon:11950;Parent=Msa0506950-mRNA-1
chr3_4 exon 53809768 53810029 53809768 ID=Msa0506950-mRNA-1:exon:11949;Parent=Msa0506950-mRNA-1
chr3_4 exon 53811202 53811480 53811202 ID=Msa0506950-mRNA-1:exon:11948;Parent=Msa0506950-mRNA-1
chr3_4 exon 53811600 53811660 53811600 ID=Msa0506950-mRNA-1:exon:11947;Parent=Msa0506950-mRNA-1
chr3_4 exon 53811742 53812248 53811742 ID=Msa0506950-mRNA-1:exon:11946;Parent=Msa0506950-mRNA-1
chr3_4 five_prime_UTR 53812092 53812248 53812092 ID=Msa0506950-mRNA-1:five_prime_utr;Parent=Msa0506950-mRNA-1
chr3_4 CDS 53811742 53812091 53811742 ID=Msa0506950-mRNA-1:cds;Parent=Msa0506950-mRNA-1
chr3_4 CDS 53811600 53811660 53811600 ID=Msa0506950-mRNA-1:cds;Parent=Msa0506950-mRNA-1
chr3_4 CDS 53811202 53811480 53811202 ID=Msa0506950-mRNA-1:cds;Parent=Msa0506950-mRNA-1
chr3_4 CDS 53809768 53810029 53809768 ID=Msa0506950-mRNA-1:cds;Parent=Msa0506950-mRNA-1
chr3_4 CDS 53809049 53809383 53809049 ID=Msa0506950-mRNA-1:cds;Parent=Msa0506950-mRNA-1
chr3_4 three_prime_UTR 53808746 53809048 53808746 ID=Msa0506950-mRNA-1:three_prime_utr;Parent=Msa0506950-mRNA-1
Gene Sequence

>Msa0506950

ATGTGGCACGGTCCTCAAGAAGTTGACAAGGTTTTAAGTCACGATGTTCCAAGCCAAGTAAACTGTGACAAGAAATGTTTTGTTCCTGTGAACTTCTCTGATTCTGAAGGGGATTCAAACTCAAATGTTGACATTCTTGATCTTTTATACGGTGGCTTTGAAAATGAAGACTGGGCTAATCCTTTAACTAGCAATGAAGATGAATGCATTTATGCTGTTCTTGGGGAAGGCTTTGCGAAAATTCTTCTCCTAAGCGATAACTATCCAAGCATACCGGCTTCTTTGCACCCTGTGCTTTTATCTAAGCTCATTTACCTGTACTTCAGTGATGTATCAGAAAATATGCACAGGTTGAAGCAATGCTTATCTGTTTTCTTTGAGCACTACCCATGTCTCTCAACAAATCACAAGAGGTGTATATTGAAGGCTTTTATTCCAGCTATGCGTTCAATGTGGCCAGGAATTTTTGGCAATTCCGGGGGTTCCCCTTTTATGGTGTCTCAGATGCGTAAGCGTGCGGTTCAAGCTTCACGATTTATGTTGCAGATGGTTCAGATTCCATTATTCGTTAAAGAGACTGAAGCAGTGAGTGAAAACTCTGGTACAGAACACCCACAAGTTATAGACAATATTGCTGAAGTTCCATTTGAGTGTGGTGAGGAGGGGCTCGCACTACGCATAGCCATAGAGGTCGCAAGCTTTCACTCGAAGAAGACAGCTGCTGAGAAGGCATATGTATCTGCATTATGCAAAATACTTGTCTCACTTCATTTTCGGTTATCAGAACAAGGGCCAATAAAAATTACGAGGAAACTTCTATGTCGTATGGCTGAATGTGTATCTTCAGAAAAGGATCTTGTTAAAGAATTGAAACGCATGGCTGATCATCTAATGACAATTGACAGGCAACAAGATCAAGAATTGTTGCAAGATGAAGTTAATCTCATTTTGGGAAAACTGGAACTCGACTTCAACCTGGATTTGGATGCTTCTGTTGCAATGCCACAAACACCAGCTGTACAGCCAACGAGAGCCACACGTGCAAGGAGAAGGGTAAGGATCGAGGAAGATAGTTCTGATGATGAAGAAGACTCGCAAGCTTCTGTGATTCCCGCCACTGTTAATACGGTAAAAGGTCGCTCGCAGAGAGCAAGCAAAACTGCAGCCATGAACAAGATGTCGTCTGCCATTAGATCACCCATAATTGATGAATTTGAAGAGCAAGAGGAAGAAGATGAAGCGTCGAATTTGACATCTGAAGATGAAGAATCCGACCAGTCTAGTTAA
Protein sequence

>Msa0506950

MWHGPQEVDKVLSHDVPSQVNCDKKCFVPVNFSDSEGDSNSNVDILDLLYGGFENEDWANPLTSNEDECIYAVLGEGFAKILLLSDNYPSIPASLHPVLLSKLIYLYFSDVSENMHRLKQCLSVFFEHYPCLSTNHKRCILKAFIPAMRSMWPGIFGNSGGSPFMVSQMRKRAVQASRFMLQMVQIPLFVKETEAVSENSGTEHPQVIDNIAEVPFECGEEGLALRIAIEVASFHSKKTAAEKAYVSALCKILVSLHFRLSEQGPIKITRKLLCRMAECVSSEKDLVKELKRMADHLMTIDRQQDQELLQDEVNLILGKLELDFNLDLDASVAMPQTPAVQPTRATRARRRVRIEEDSSDDEEDSQASVIPATVNTVKGRSQRASKTAAMNKMSSAIRSPIIDEFEEQEEEDEASNLTSEDEESDQSS*