AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0154620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154620 MtrunA17_Chr1g0165201 96.829 473 11 2 1 473 1 469 0.0 932
Msa0154620 MtrunA17_Chr1g0212321 65.227 463 136 7 28 473 32 486 0.0 580
Msa0154620 MtrunA17_Chr1g0210201 49.367 395 187 4 79 473 86 467 2.34e-125 373
Msa0154620 MtrunA17_Chr7g0275731 46.445 422 213 6 55 473 73 484 1.83e-118 355
Msa0154620 MtrunA17_Chr6g0484901 41.650 497 253 13 2 473 7 491 2.72e-107 327
Msa0154620 MtrunA17_Chr3g0130931 38.018 434 238 13 50 473 36 448 1.55e-84 267
Msa0154620 MtrunA17_Chr8g0390761 37.209 344 210 4 125 466 109 448 1.02e-78 252
Msa0154620 MtrunA17_Chr4g0001991 32.767 412 254 7 75 473 116 517 6.66e-68 226
Msa0154620 MtrunA17_Chr8g0386441 33.333 429 245 16 59 473 28 429 4.10e-67 221
Msa0154620 MtrunA17_Chr5g0435871 33.179 431 245 14 59 473 31 434 4.66e-67 221
Msa0154620 MtrunA17_Chr5g0435971 33.411 431 244 14 59 473 28 431 7.02e-67 221
Msa0154620 MtrunA17_Chr3g0099891 36.508 378 217 9 107 473 89 454 3.05e-66 219
Msa0154620 MtrunA17_Chr3g0095891 32.102 433 251 8 76 473 34 458 4.61e-66 219
Msa0154620 MtrunA17_Chr2g0291201 31.453 461 277 16 31 473 12 451 4.75e-66 219
Msa0154620 MtrunA17_Chr5g0419291 35.040 371 211 12 121 473 70 428 2.00e-64 214
Msa0154620 MtrunA17_Chr3g0099901 40.278 360 197 9 124 473 134 485 2.89e-62 210
Msa0154620 MtrunA17_Chr5g0442921 31.489 470 270 12 53 473 87 553 3.69e-61 209
Msa0154620 MtrunA17_Chr6g0486981 30.115 435 258 16 59 473 28 436 1.40e-59 202
Msa0154620 MtrunA17_Chr8g0352671 31.651 436 255 16 55 473 27 436 7.92e-59 199
Msa0154620 MtrunA17_Chr8g0352661 33.967 368 202 16 131 473 85 436 2.67e-58 198
Msa0154620 MtrunA17_Chr6g0487471 33.643 431 246 15 59 473 28 434 3.19e-58 198
Msa0154620 MtrunA17_Chr2g0333991 29.882 425 259 16 62 470 31 432 5.32e-58 197
Msa0154620 MtrunA17_Chr2g0311451 29.837 429 260 17 62 473 31 435 5.15e-56 192
Msa0154620 MtrunA17_Chr2g0307991 33.795 361 205 13 132 473 59 404 6.12e-53 183
Msa0154620 MtrunA17_Chr2g0308001 32.493 357 220 13 132 473 58 408 2.91e-50 177
Msa0154620 MtrunA17_Chr3g0087851 32.133 361 218 12 130 473 86 436 1.23e-49 175
Msa0154620 MtrunA17_Chr2g0310831 30.874 366 214 14 130 473 92 440 7.76e-49 173
Msa0154620 MtrunA17_Chr2g0310821 31.638 354 213 12 131 473 107 442 1.15e-48 173
Msa0154620 MtrunA17_Chr4g0061641 35.072 345 187 13 148 473 70 396 9.84e-48 169
Msa0154620 MtrunA17_Chr5g0395931 31.956 363 213 12 131 473 87 435 1.09e-47 170
Msa0154620 MtrunA17_Chr5g0395911 32.493 357 218 11 131 473 66 413 1.25e-47 169
Msa0154620 MtrunA17_Chr2g0310801 30.411 365 212 13 131 473 89 433 1.19e-45 164
Msa0154620 MtrunA17_Chr5g0415951 30.618 356 222 14 133 473 89 434 5.32e-45 162
Msa0154620 MtrunA17_Chr5g0395871 32.961 358 214 12 130 473 79 424 6.32e-45 162
Msa0154620 MtrunA17_Chr2g0307461 31.793 368 216 12 133 473 71 430 7.82e-44 159
Msa0154620 MtrunA17_Chr3g0133071 31.144 411 247 13 75 473 51 437 5.20e-42 154
Msa0154620 MtrunA17_Chr6g0457401 29.095 409 250 15 82 473 51 436 7.41e-42 154
Msa0154620 MtrunA17_Chr1g0164401 33.333 354 211 9 130 473 90 428 2.56e-41 152
Msa0154620 MtrunA17_Chr4g0023351 37.963 216 120 7 132 334 58 272 1.83e-40 147
Msa0154620 MtrunA17_Chr8g0353261 33.048 351 217 9 130 473 94 433 1.65e-37 142
Msa0154620 MtrunA17_Chr2g0312941 29.815 379 217 12 130 471 85 451 6.37e-36 138
Msa0154620 MtrunA17_Chr7g0265331 27.168 346 218 12 131 450 80 417 2.89e-30 123
Msa0154620 MtrunA17_Chr2g0309561 25.397 441 272 14 87 473 71 508 7.65e-30 122
Msa0154620 MtrunA17_Chr1g0179091 27.869 366 196 15 129 473 48 366 6.34e-29 117
Msa0154620 MtrunA17_Chr7g0267171 27.949 390 221 15 131 473 89 465 4.00e-28 116
Msa0154620 MtrunA17_Chr8g0352691 32.463 268 162 10 215 473 2 259 8.71e-28 111
Msa0154620 MtrunA17_Chr2g0320621 28.329 353 206 17 131 450 76 414 1.01e-27 115
Msa0154620 MtrunA17_Chr3g0112051 28.689 366 202 14 135 473 76 409 1.04e-27 114
Msa0154620 MtrunA17_Chr5g0395891 29.836 305 184 12 187 473 20 312 4.60e-27 110
Msa0154620 MtrunA17_Chr3g0082281 33.688 282 166 7 202 473 49 319 6.38e-27 110
Msa0154620 MtrunA17_Chr4g0027681 28.771 358 199 19 130 450 75 413 3.27e-26 111
Msa0154620 MtrunA17_Chr4g0056211 28.180 401 213 19 137 473 81 470 4.91e-26 110
Msa0154620 MtrunA17_Chr5g0401601 25.067 371 233 14 130 473 86 438 1.05e-25 110
Msa0154620 MtrunA17_Chr4g0053721 24.528 371 235 14 130 473 78 430 3.60e-25 108
Msa0154620 MtrunA17_Chr7g0239001 28.800 375 220 18 135 473 62 425 9.27e-25 106
Msa0154620 MtrunA17_Chr3g0110191 26.902 368 222 15 135 473 89 438 1.73e-24 105
Msa0154620 MtrunA17_Chr8g0384731 26.075 372 227 15 131 473 72 424 2.38e-24 105
Msa0154620 MtrunA17_Chr5g0399171 25.666 413 219 17 132 473 74 469 3.59e-24 105
Msa0154620 MtrunA17_Chr1g0189381 25.926 378 231 16 130 473 67 429 1.07e-23 103
Msa0154620 MtrunA17_Chr7g0221681 24.416 385 241 14 124 473 64 433 1.97e-23 102
Msa0154620 MtrunA17_Chr1g0179751 26.136 352 220 12 129 450 105 446 3.08e-23 102
Msa0154620 MtrunA17_Chr5g0419151 28.832 274 175 9 208 473 106 367 8.71e-23 99.8
Msa0154620 MtrunA17_Chr5g0434411 25.500 400 257 13 102 473 51 437 4.55e-22 98.6
Msa0154620 MtrunA17_Chr7g0249451 25.067 375 239 15 124 468 63 425 1.29e-21 97.4
Msa0154620 MtrunA17_Chr8g0352681 33.108 148 84 4 62 209 32 164 4.14e-20 87.4
Msa0154620 MtrunA17_Chr5g0406001 24.665 373 225 12 133 473 113 461 3.00e-19 90.5
Msa0154620 MtrunA17_Chr3g0124421 23.438 448 292 15 56 473 24 450 4.57e-19 90.1
Msa0154620 MtrunA17_Chr3g0130741 25.654 382 231 17 123 473 75 434 5.16e-19 89.4
Msa0154620 MtrunA17_Chr5g0425141 25.618 445 269 20 60 473 36 449 6.49e-19 89.4
Msa0154620 MtrunA17_Chr8g0371351 23.913 368 234 15 133 473 101 449 5.19e-18 86.7
Msa0154620 MtrunA17_Chr4g0010951 31.064 235 130 10 102 312 31 257 1.62e-17 84.7
Msa0154620 MtrunA17_Chr5g0434441 25.466 322 212 11 135 435 71 385 1.82e-17 84.7
Msa0154620 MtrunA17_Chr6g0484731 23.821 424 265 19 89 473 146 550 2.18e-17 84.7
Msa0154620 MtrunA17_Chr3g0078321 22.423 388 250 14 118 473 44 412 2.24e-14 75.1
Msa0154620 MtrunA17_Chr2g0287821 23.604 394 239 18 127 472 63 442 4.38e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154620 AT3G20015.1 60.626 447 171 4 29 473 27 470 0.0 524
Msa0154620 AT1G25510.1 43.833 454 237 7 32 473 36 483 3.87e-125 373
Msa0154620 AT3G18490.1 42.800 500 250 10 2 473 9 500 7.97e-123 368
Msa0154620 AT1G01300.1 48.418 411 205 6 67 473 77 484 3.46e-118 355
Msa0154620 AT3G61820.1 48.010 402 197 5 83 473 83 483 3.88e-116 350
Msa0154620 AT5G10770.1 35.377 424 243 9 72 473 59 473 1.57e-76 247
Msa0154620 AT2G03200.1 35.802 405 234 13 86 473 63 458 3.01e-70 231
Msa0154620 AT5G10760.1 36.923 390 229 9 87 473 89 464 1.20e-65 219
Msa0154620 AT5G33340.1 31.735 438 260 12 50 473 22 434 6.36e-63 211
Msa0154620 AT1G79720.1 33.415 410 240 10 87 473 83 482 1.62e-59 203
Msa0154620 AT3G59080.1 31.651 436 238 12 89 473 106 532 1.02e-56 197
Msa0154620 AT2G42980.1 32.054 443 256 14 72 473 86 524 1.16e-56 196
Msa0154620 AT3G25700.1 32.895 380 228 11 120 473 71 449 7.69e-55 190
Msa0154620 AT1G64830.1 32.118 439 256 15 50 473 17 428 1.37e-54 189
Msa0154620 AT2G28040.2 33.333 351 201 10 132 473 55 381 7.21e-52 180
Msa0154620 AT2G28040.1 33.333 351 201 10 132 473 64 390 1.25e-51 180
Msa0154620 AT3G54400.1 34.091 352 212 10 130 473 85 424 7.00e-50 176
Msa0154620 AT1G09750.1 31.956 363 229 10 122 473 93 448 7.39e-50 176
Msa0154620 AT2G28010.1 29.716 387 238 9 96 473 30 391 9.42e-49 172
Msa0154620 AT3G59080.2 30.626 431 213 12 89 473 106 496 1.20e-47 172
Msa0154620 AT2G28030.1 31.143 350 209 8 133 473 61 387 6.15e-47 167
Msa0154620 AT2G28220.1 30.447 358 205 11 133 473 67 397 8.26e-44 159
Msa0154620 AT2G35615.1 27.991 443 266 17 59 473 26 443 8.26e-43 157
Msa0154620 AT1G31450.1 29.268 451 263 18 50 473 20 441 1.18e-42 157
Msa0154620 AT4G30040.1 31.214 346 209 10 133 460 85 419 5.33e-39 146
Msa0154620 AT2G28225.1 29.722 360 205 13 133 473 67 397 5.87e-39 146
Msa0154620 AT5G07030.1 33.053 357 212 10 130 473 112 454 1.08e-37 143
Msa0154620 AT2G23945.1 29.000 400 241 17 62 440 32 409 8.52e-37 141
Msa0154620 AT3G12700.1 26.531 441 283 16 52 473 42 460 2.09e-33 132
Msa0154620 AT4G30030.1 31.014 345 199 15 133 457 78 403 2.20e-32 128
Msa0154620 AT5G43100.1 27.548 363 226 13 130 473 73 417 4.60e-32 129
Msa0154620 AT5G43100.2 27.548 363 226 13 130 473 73 417 6.97e-32 127
Msa0154620 AT2G36670.2 27.586 348 213 13 131 450 98 434 1.69e-31 127
Msa0154620 AT2G36670.4 27.457 346 212 13 133 450 34 368 3.45e-31 125
Msa0154620 AT2G36670.3 27.457 346 212 13 133 450 34 368 3.45e-31 125
Msa0154620 AT2G36670.1 27.457 346 212 13 133 450 105 439 4.25e-31 125
Msa0154620 AT3G02740.1 27.733 375 227 15 128 473 80 439 7.23e-31 125
Msa0154620 AT3G02740.2 27.568 370 224 15 133 473 10 364 2.61e-30 122
Msa0154620 AT2G39710.1 28.571 378 215 16 135 472 67 429 9.30e-30 121
Msa0154620 AT3G50050.1 28.056 360 230 13 130 473 90 436 5.77e-29 120
Msa0154620 AT1G05840.1 26.963 382 233 15 124 473 71 438 1.81e-27 115
Msa0154620 AT5G36260.1 25.474 369 238 13 131 473 76 433 2.05e-27 115
Msa0154620 AT1G66180.1 28.022 364 226 13 135 473 74 426 4.17e-26 110
Msa0154620 AT5G22850.1 28.286 350 210 14 131 450 79 417 5.26e-26 110
Msa0154620 AT5G37540.1 27.671 365 230 12 135 473 82 438 5.51e-26 110
Msa0154620 AT1G65240.1 24.866 374 231 15 131 473 72 426 4.09e-25 108
Msa0154620 AT3G52500.1 27.317 410 240 15 110 473 70 467 3.10e-24 105
Msa0154620 AT5G02190.1 28.184 369 208 18 148 473 88 442 5.01e-24 104
Msa0154620 AT4G12920.1 28.000 350 197 11 133 460 58 374 1.09e-22 100
Msa0154620 AT1G08210.4 27.224 371 230 16 131 473 116 474 3.26e-22 100
Msa0154620 AT1G08210.3 27.224 371 230 16 131 473 130 488 3.27e-22 100
Msa0154620 AT5G45120.1 23.326 463 279 18 80 473 23 478 3.90e-22 99.4
Msa0154620 AT1G08210.1 27.224 371 230 16 131 473 82 440 5.83e-22 99.0
Msa0154620 AT1G08210.2 28.527 319 191 14 131 424 82 388 1.44e-21 97.8
Msa0154620 AT3G25700.2 33.537 164 95 5 120 269 71 234 1.06e-18 87.8
Msa0154620 AT1G77480.2 27.013 385 225 20 123 473 57 419 1.07e-18 88.6
Msa0154620 AT4G16563.1 27.136 398 210 16 150 473 100 491 1.25e-18 89.0
Msa0154620 AT4G33490.2 28.897 263 149 12 123 368 50 291 1.68e-18 88.2
Msa0154620 AT1G77480.1 27.013 385 225 20 123 473 57 419 1.68e-18 88.2
Msa0154620 AT4G33490.1 28.897 263 149 12 123 368 47 288 1.80e-18 87.8
Msa0154620 AT1G49050.2 27.132 258 154 13 133 369 30 274 3.85e-18 86.7
Msa0154620 AT3G51340.2 25.886 367 224 18 133 473 103 447 4.15e-17 84.3
Msa0154620 AT4G35880.1 24.217 351 224 14 133 460 107 438 1.25e-16 82.8
Msa0154620 AT3G51350.1 24.518 363 217 17 133 468 102 434 7.56e-16 80.5
Msa0154620 AT4G35880.2 24.189 339 215 14 133 448 107 426 2.98e-15 78.2
Msa0154620 AT3G42550.1 24.930 357 213 15 130 473 75 389 4.27e-15 77.8
Msa0154620 AT3G51340.3 25.566 309 188 14 133 418 103 392 4.80e-15 77.4
Msa0154620 AT1G44130.1 31.068 206 115 10 123 310 39 235 7.90e-15 76.6
Msa0154620 AT3G51360.9 25.641 351 211 16 83 413 62 382 3.68e-14 75.1
Msa0154620 AT3G51360.4 25.067 371 228 16 83 433 52 392 3.74e-14 74.7
Msa0154620 AT3G51360.5 25.067 371 228 16 83 433 62 402 3.75e-14 74.7
Msa0154620 AT3G51360.10 25.641 351 211 16 83 413 52 372 3.85e-14 74.7
Msa0154620 AT3G51360.1 25.641 351 211 16 83 413 52 372 4.20e-14 74.7
Msa0154620 AT3G51360.6 25.641 351 211 16 83 413 62 382 5.28e-14 74.3
Msa0154620 AT3G51340.4 25.175 286 175 13 133 395 103 372 3.12e-13 71.6
Msa0154620 AT3G51340.1 25.175 286 175 13 133 395 103 372 3.58e-13 72.0
Msa0154620 AT3G51360.7 26.246 301 185 14 133 413 5 288 1.19e-12 70.1
Msa0154620 AT3G51360.2 25.574 305 179 14 83 369 62 336 1.48e-11 66.2

Find 137 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATATTTGTTCGGATA+TGG 0.122611 1_4:-19335686 None:intergenic
GCTACTTCTGCTTCGTTTAT+TGG 0.138095 1_4:+19334219 Msa0154620:CDS
TTCTACTACGTCTCACTTTC+GGG 0.179528 1_4:+19335637 Msa0154620:CDS
CTACTTCTGCTTCGTTTATT+GGG 0.181833 1_4:+19334220 Msa0154620:CDS
ACTAAAACAATCTCGAATTT+TGG 0.205561 1_4:+19336130 Msa0154620:three_prime_UTR
GATATCCACACCTCCCTTTC+CGG 0.230477 1_4:-19334293 None:intergenic
ATCCAAAGGCTCCGCCGGTT+TGG 0.265743 1_4:-19335501 None:intergenic
CTTCTACTACGTCTCACTTT+CGG 0.273039 1_4:+19335636 Msa0154620:CDS
TTTGGATCTGATGTAGTTTC+AGG 0.277753 1_4:+19334039 Msa0154620:CDS
CCCTCTGGTCTTTCCATCAT+AGG 0.282371 1_4:+19336006 Msa0154620:CDS
TCCAAAGGCTCCGCCGGTTT+GGG 0.283928 1_4:-19335500 None:intergenic
GGAATGTTTGTTGGAGCAGC+TGG 0.314111 1_4:+19335427 Msa0154620:CDS
ATTCAGGAAGTGATATTGTT+TGG 0.328169 1_4:+19334139 Msa0154620:CDS
GCCCAAACCGGCGGAGCCTT+TGG 0.328588 1_4:+19335499 Msa0154620:CDS
AAGTGGTTAGTGCTGTTATT+AGG 0.330467 1_4:-19333720 None:intergenic
GAGGTTGTTGTTGGGTATTA+TGG 0.333275 1_4:-19333837 None:intergenic
GCAGCTGGGTTGTTAGGCCT+TGG 0.336549 1_4:+19335442 Msa0154620:CDS
GTTTCTATCGATGGAACTAA+TGG 0.337355 1_4:+19336054 Msa0154620:CDS
AACGGTCCGCCCGATGGTTA+TGG 0.339939 1_4:-19335365 None:intergenic
TATGTCTTTCGTGGGCCAAT+TGG 0.346916 1_4:+19335474 Msa0154620:CDS
GTTGGAGCAGCTGGGTTGTT+AGG 0.347858 1_4:+19335436 Msa0154620:CDS
ACAGCAACAGAAGCTAGTTT+TGG 0.352053 1_4:+19334021 Msa0154620:CDS
CGCCGGTTTGGGCTCCCAAT+TGG 0.356798 1_4:-19335489 None:intergenic
TGAAATATTTGTTCGGATAT+GGG 0.367001 1_4:-19335685 None:intergenic
GCATTTGCTCCTTCACCCTC+TGG 0.370463 1_4:+19335991 Msa0154620:CDS
TCCTGCTATTTATCAGTATA+TGG 0.379135 1_4:+19334110 Msa0154620:CDS
ATGTCTTTCGTGGGCCAATT+GGG 0.379833 1_4:+19335475 Msa0154620:CDS
CAGTATATGGTGATAGATTC+AGG 0.394103 1_4:+19334123 Msa0154620:CDS
TTAGTGCTGTTATTAGGATT+AGG 0.408484 1_4:-19333714 None:intergenic
ATATTTCAGTTAACTGATAT+AGG 0.421183 1_4:+19335700 Msa0154620:CDS
TCACTTTCGGGTCTTGCAGT+TGG 0.424557 1_4:+19335649 Msa0154620:CDS
CTTTCGGGTCTTGCAGTTGG+AGG 0.428746 1_4:+19335652 Msa0154620:CDS
TCATCCGGTTCACTCGAATT+CGG 0.429351 1_4:+19335550 Msa0154620:CDS
TGGAACGAGGGAATGTTTGT+TGG 0.432846 1_4:+19335418 Msa0154620:CDS
AAGTGGTGTTGGAAAGGAAA+TGG 0.433318 1_4:-19333743 None:intergenic
TTGCTGAATATTCCCTATGA+TGG 0.435218 1_4:-19336019 None:intergenic
TTGATGATGATGTTGCTTGC+CGG 0.437671 1_4:+19334274 Msa0154620:CDS
CAGTTAACTGATATAGGAAC+CGG 0.438789 1_4:+19335706 Msa0154620:CDS
TTGCACCTACGGGCATTGCT+TGG 0.450316 1_4:-19335576 None:intergenic
TGCATCGCGAAAAGCATTGT+AGG 0.452930 1_4:-19335779 None:intergenic
CTGCAATCGGGTGCGGACAT+TGG 0.452938 1_4:+19335398 Msa0154620:CDS
TGTCTTATCTCTCGAGGCAC+TGG 0.460971 1_4:+19335526 Msa0154620:CDS
GGCCAATTGGGAGCCCAAAC+CGG 0.466739 1_4:+19335487 Msa0154620:CDS
AATAAATGTGTTGATACAAA+AGG 0.472085 1_4:-19336170 None:intergenic
AGTTAACTGAAATATTTGTT+CGG 0.472372 1_4:-19335692 None:intergenic
GCTGGGTTGTTAGGCCTTGG+AGG 0.477592 1_4:+19335445 Msa0154620:CDS
TTCTGCTTCGTTTATTGGGG+TGG 0.480733 1_4:+19334224 Msa0154620:CDS
TTACTGAGTGTGTTGGTGGT+TGG 0.481231 1_4:-19333652 None:intergenic
GGAATTCAAGTTTCTATCGA+TGG 0.482938 1_4:+19336045 Msa0154620:CDS
GAGTGAGGACTGAGGAATGA+TGG 0.485034 1_4:-19333618 None:intergenic
TGTTTGCTTCAACACACATT+GGG 0.485439 1_4:-19336093 None:intergenic
AGTGTGTTGGTGGTTGGTGA+AGG 0.486160 1_4:-19333646 None:intergenic
TTGATCCCGGCCGATGATGT+TGG 0.486689 1_4:+19335955 Msa0154620:CDS
GATTGCAGTGTCCTTGATAA+CGG 0.491062 1_4:-19335383 None:intergenic
ATACCCAACAACAACCTCAA+TGG 0.495019 1_4:+19333842 Msa0154620:CDS
AGGCGGACCTATGTCTTTCG+TGG 0.496338 1_4:+19335465 Msa0154620:CDS
TCTCTTACTGAGTGTGTTGG+TGG 0.501582 1_4:-19333656 None:intergenic
AGTGAGACGTAGTAGAAGCT+AGG 0.502354 1_4:-19335631 None:intergenic
GTTATCAAGGACACTGCAAT+CGG 0.503421 1_4:+19335385 Msa0154620:CDS
ATTAGGATTAGGAGTAGAAG+AGG 0.503521 1_4:-19333703 None:intergenic
AAATCTTGACCTTTCCAGCC+AGG 0.503991 1_4:+19335926 Msa0154620:CDS
AGTAGAAGCTAGGGTAGAAC+GGG 0.505044 1_4:-19335621 None:intergenic
GCGTCCGAATTCGAGTGAAC+CGG 0.505443 1_4:-19335554 None:intergenic
GTGAGACGTAGTAGAAGCTA+GGG 0.505740 1_4:-19335630 None:intergenic
ATAAACGAAGCAGAAGTAGC+AGG 0.508250 1_4:-19334216 None:intergenic
TTTCCATTGAGGTTGTTGTT+GGG 0.509707 1_4:-19333845 None:intergenic
TCTCGAGGCACTGGATCATC+CGG 0.510100 1_4:+19335535 Msa0154620:CDS
CTCTTGAAACCATAACCATC+GGG 0.512781 1_4:+19335356 Msa0154620:CDS
CATCGGGCGGACCGTTATCA+AGG 0.514418 1_4:+19335372 Msa0154620:CDS
AGGAACCCACATTGCACCTA+CGG 0.516231 1_4:-19335587 None:intergenic
CCTCTGGTCTTTCCATCATA+GGG 0.517585 1_4:+19336007 Msa0154620:CDS
AGGTGTTGGAAGTGAGAAAG+TGG 0.522851 1_4:-19333760 None:intergenic
CTTGATAACGGTCCGCCCGA+TGG 0.523145 1_4:-19335371 None:intergenic
ATGATGTTGCTTGCCGGAAA+GGG 0.527421 1_4:+19334280 Msa0154620:CDS
GTGTGTTGGTGGTTGGTGAA+GGG 0.527741 1_4:-19333645 None:intergenic
AAAGTTCCAACATCATCGGC+CGG 0.529607 1_4:-19335961 None:intergenic
TGCTCTCTTACTGAGTGTGT+TGG 0.531988 1_4:-19333659 None:intergenic
AACAGTAGGTAACCTGGTAA+TGG 0.534799 1_4:-19335755 None:intergenic
ACAGTATCCAAAGGCTCCGC+CGG 0.535096 1_4:-19335506 None:intergenic
GTAGTTTCAGGCACTGAAGA+AGG 0.536968 1_4:+19334051 Msa0154620:CDS
TACTTCTGCTTCGTTTATTG+GGG 0.538816 1_4:+19334221 Msa0154620:CDS
AAGACATAGGTCCGCCTCCA+AGG 0.540966 1_4:-19335459 None:intergenic
GGAACCCACATTGCACCTAC+GGG 0.543677 1_4:-19335586 None:intergenic
GGGTTGTTAGGCCTTGGAGG+CGG 0.545043 1_4:+19335448 Msa0154620:CDS
TGGAAGTGAGAAAGTGGTGT+TGG 0.549954 1_4:-19333754 None:intergenic
GGCGGACCTATGTCTTTCGT+GGG 0.550117 1_4:+19335466 Msa0154620:CDS
ACCATATACTGATAAATAGC+AGG 0.551497 1_4:-19334111 None:intergenic
GTGAGAAAGTGGTGTTGGAA+AGG 0.553735 1_4:-19333749 None:intergenic
GAATGTTTGTTGGAGCAGCT+GGG 0.555216 1_4:+19335428 Msa0154620:CDS
GCTCTTGAAACCATAACCAT+CGG 0.557569 1_4:+19335355 Msa0154620:CDS
GACACGTGTTATGACTTGAA+CGG 0.559620 1_4:+19335853 Msa0154620:CDS
GAAATATTTGTTCGGATATG+GGG 0.560097 1_4:-19335684 None:intergenic
AATTGGCCCACGAAAGACAT+AGG 0.560178 1_4:-19335472 None:intergenic
TGTGGATATCAAGTTGCATA+TGG 0.560299 1_4:+19334306 Msa0154620:CDS
GGTGTCCATGACAACTCCTC+CGG 0.560381 1_4:-19335725 None:intergenic
ACACCGGCACAGCCATTACC+AGG 0.562171 1_4:+19335743 Msa0154620:CDS
GATGATGTTGCTTGCCGGAA+AGG 0.565770 1_4:+19334279 Msa0154620:CDS
TAGTAGAAGCTAGGGTAGAA+CGG 0.569534 1_4:-19335622 None:intergenic
ATGTTTGCTTCAACACACAT+TGG 0.570489 1_4:-19336094 None:intergenic
AAGTTCCAACATCATCGGCC+GGG 0.575288 1_4:-19335960 None:intergenic
ATCAAGTTGCATATGGTGAT+GGG 0.575723 1_4:+19334313 Msa0154620:CDS
TTAGGATTAGGAGTAGAAGA+GGG 0.579457 1_4:-19333702 None:intergenic
TCAGGCACTGAAGAAGGAAG+TGG 0.583731 1_4:+19334057 Msa0154620:CDS
AAGGACACTGCAATCGGGTG+CGG 0.585643 1_4:+19335391 Msa0154620:CDS
TATCAAGTTGCATATGGTGA+TGG 0.587507 1_4:+19334312 Msa0154620:CDS
GGATACTGTCTTATCTCTCG+AGG 0.592311 1_4:+19335520 Msa0154620:CDS
ACAGAAAGTTCCAACATCAT+CGG 0.592640 1_4:-19335965 None:intergenic
TAAACGAAGCAGAAGTAGCA+GGG 0.592676 1_4:-19334215 None:intergenic
ATGGAAAGACCAGAGGGTGA+AGG 0.594528 1_4:-19336000 None:intergenic
TGATTATAACATTGATCACA+AGG 0.597383 1_4:-19334174 None:intergenic
ATAGGGAATATTCAGCAAGA+AGG 0.599831 1_4:+19336024 Msa0154620:CDS
TAACCTGGTAATGGCTGTGC+CGG 0.602170 1_4:-19335746 None:intergenic
AAAGCATTGTAGGCAACAGT+AGG 0.603981 1_4:-19335769 None:intergenic
GTAGGCAACAGTAGGTAACC+TGG 0.609612 1_4:-19335761 None:intergenic
AGGAACCGGAGGAGTTGTCA+TGG 0.611224 1_4:+19335720 Msa0154620:CDS
TTATCAAGGACACTGCAATC+GGG 0.611505 1_4:+19335386 Msa0154620:CDS
CAATGCCCGTAGGTGCAATG+TGG 0.615447 1_4:+19335581 Msa0154620:CDS
GGAGGAGTTGTCATGGACAC+CGG 0.619800 1_4:+19335727 Msa0154620:CDS
TTGGTGAAGGGATTTGAGTG+AGG 0.624084 1_4:-19333633 None:intergenic
AATGCCCGTAGGTGCAATGT+GGG 0.625127 1_4:+19335582 Msa0154620:CDS
GGTGATGGGTCGTCTACCAA+AGG 0.626771 1_4:+19334327 Msa0154620:CDS
CCCTATGATGGAAAGACCAG+AGG 0.627903 1_4:-19336007 None:intergenic
ATAGAAACTCTGGGAGCACG+TGG 0.629230 1_4:-19335826 None:intergenic
AGGGATTTGAGTGAGGACTG+AGG 0.629426 1_4:-19333626 None:intergenic
CGACACTGTTGGAACTCGAA+CGG 0.632988 1_4:-19335884 None:intergenic
TGTTGGAAAGGAAATGGAAG+TGG 0.637079 1_4:-19333737 None:intergenic
GGACGCCAAGCAATGCCCGT+AGG 0.637191 1_4:+19335571 Msa0154620:CDS
ATGTTGCTTGCCGGAAAGGG+AGG 0.650168 1_4:+19334283 Msa0154620:CDS
GGGTGCGGACATTGGAACGA+GGG 0.652299 1_4:+19335406 Msa0154620:CDS
CAATTGGGAGCCCAAACCGG+CGG 0.653313 1_4:+19335490 Msa0154620:CDS
CGGGTGCGGACATTGGAACG+AGG 0.653686 1_4:+19335405 Msa0154620:CDS
CCTATGATGGAAAGACCAGA+GGG 0.661561 1_4:-19336006 None:intergenic
TAGAACGGGTTATGAATGAG+AGG 0.671064 1_4:-19335607 None:intergenic
AGAGATAAGACAGTATCCAA+AGG 0.673865 1_4:-19335515 None:intergenic
TTAACTGATATAGGAACCGG+AGG 0.677821 1_4:+19335709 Msa0154620:CDS
TTGAAACCATAACCATCGGG+CGG 0.703144 1_4:+19335359 Msa0154620:CDS
GCTTGCCGGAAAGGGAGGTG+TGG 0.712007 1_4:+19334288 Msa0154620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGAAAATTTTAAAATCAAT+TGG - chr1_4:19335200-19335219 None:intergenic 10.0%
!!! TTTTAGATGATGTTTATATT+AGG + chr1_4:19334464-19334483 Msa0154620:intron 15.0%
!!! AGAATTTAATTTAACATGTT+AGG + chr1_4:19334533-19334552 Msa0154620:intron 15.0%
!! TGATAAAGAATTAATTGTAA+AGG + chr1_4:19334867-19334886 Msa0154620:intron 15.0%
!!! AAGTTAGAATTTTTAGATTA+TGG + chr1_4:19334964-19334983 Msa0154620:intron 15.0%
!! AAAATAAAGTGATTTATGTT+TGG - chr1_4:19335131-19335150 None:intergenic 15.0%
!! TCTTTATGTAATCTAATTAT+TGG + chr1_4:19335306-19335325 Msa0154620:intron 15.0%
!!! TTGTTTTTTTGGTGTTTTTA+AGG - chr1_4:19333900-19333919 None:intergenic 20.0%
!!! TATTTTGTTAGAATAGGAAT+TGG + chr1_4:19334084-19334103 Msa0154620:CDS 20.0%
!!! AGACAAAATTGATTCTAAAA+GGG + chr1_4:19334506-19334525 Msa0154620:intron 20.0%
!!! TGTATGTTTGGTTTTATTTT+TGG + chr1_4:19334843-19334862 Msa0154620:intron 20.0%
!!! ATTAGCTTGATTCTATAAAA+TGG - chr1_4:19335173-19335192 None:intergenic 20.0%
!! AGTTAACTGAAATATTTGTT+CGG - chr1_4:19335695-19335714 None:intergenic 20.0%
!! ATATTTCAGTTAACTGATAT+AGG + chr1_4:19335700-19335719 Msa0154620:CDS 20.0%
!!! AATAAATGTGTTGATACAAA+AGG - chr1_4:19336173-19336192 None:intergenic 20.0%
!!! TTTATAGTTAGCTTTTTCTA+TGG + chr1_4:19336220-19336239 Msa0154620:three_prime_UTR 20.0%
!! GGTGAATATTTTGTTAGAAT+AGG + chr1_4:19334078-19334097 Msa0154620:CDS 25.0%
! TGATTATAACATTGATCACA+AGG - chr1_4:19334177-19334196 None:intergenic 25.0%
!! GAGACAAAATTGATTCTAAA+AGG + chr1_4:19334505-19334524 Msa0154620:intron 25.0%
!! AATTATCATTTCCCCTTTTT+CGG - chr1_4:19334773-19334792 None:intergenic 25.0%
!!! GGTGTAAAATTGATTTTGAT+AGG + chr1_4:19334888-19334907 Msa0154620:intron 25.0%
!!! GAATTTTTAGATTATGGCTA+AGG + chr1_4:19334970-19334989 Msa0154620:intron 25.0%
!!! TGTTTTAAGTACATTGTTCA+AGG - chr1_4:19335085-19335104 None:intergenic 25.0%
! AAAGAGTAGAATAAAATGCA+TGG + chr1_4:19335281-19335300 Msa0154620:intron 25.0%
! AAGAGTAGAATAAAATGCAT+GGG + chr1_4:19335282-19335301 Msa0154620:intron 25.0%
! TGAAATATTTGTTCGGATAT+GGG - chr1_4:19335688-19335707 None:intergenic 25.0%
!!! AAAATTCGAGATTGTTTTAG+TGG - chr1_4:19336131-19336150 None:intergenic 25.0%
!! ACTAAAACAATCTCGAATTT+TGG + chr1_4:19336130-19336149 Msa0154620:three_prime_UTR 25.0%
! TTAGTGCTGTTATTAGGATT+AGG - chr1_4:19333717-19333736 None:intergenic 30.0%
! AGATTGCGTTTTCTACATTT+AGG - chr1_4:19333783-19333802 None:intergenic 30.0%
!!! AACAGTTTTGTTTTCCATTG+AGG - chr1_4:19333859-19333878 None:intergenic 30.0%
!!! TGTTTTGTGGGTTGTTTTTT+TGG - chr1_4:19333911-19333930 None:intergenic 30.0%
! TCCTGCTATTTATCAGTATA+TGG + chr1_4:19334110-19334129 Msa0154620:CDS 30.0%
ACCATATACTGATAAATAGC+AGG - chr1_4:19334114-19334133 None:intergenic 30.0%
ATTCAGGAAGTGATATTGTT+TGG + chr1_4:19334139-19334158 Msa0154620:CDS 30.0%
AACAAAGCAAGAAAAATGTG+TGG - chr1_4:19334388-19334407 None:intergenic 30.0%
!! TAGGTTCTACTGTATTTTAG+AGG + chr1_4:19334483-19334502 Msa0154620:intron 30.0%
ATTCACATTGAGTGTATGTT+TGG + chr1_4:19334831-19334850 Msa0154620:intron 30.0%
AGAGTAGAATAAAATGCATG+GGG + chr1_4:19335283-19335302 Msa0154620:intron 30.0%
! TTGGTTTCCAATGTTGATAA+AGG + chr1_4:19335325-19335344 Msa0154620:intron 30.0%
! TGGTTTCCAATGTTGATAAA+GGG + chr1_4:19335326-19335345 Msa0154620:intron 30.0%
GAAATATTTGTTCGGATATG+GGG - chr1_4:19335687-19335706 None:intergenic 30.0%
CTGAAATATTTGTTCGGATA+TGG - chr1_4:19335689-19335708 None:intergenic 30.0%
GTGTCAAAAATAGAAACTCT+GGG - chr1_4:19335838-19335857 None:intergenic 30.0%
GAAAAATAAAACGACACTGT+TGG - chr1_4:19335898-19335917 None:intergenic 30.0%
!!! ACAGTGTCGTTTTATTTTTC+AGG + chr1_4:19335898-19335917 Msa0154620:CDS 30.0%
!!! ACTAATGGTTTTGTAGGATT+TGG + chr1_4:19336069-19336088 Msa0154620:CDS 30.0%
TTAGGATTAGGAGTAGAAGA+GGG - chr1_4:19333705-19333724 None:intergenic 35.0%
ATTAGGATTAGGAGTAGAAG+AGG - chr1_4:19333706-19333725 None:intergenic 35.0%
! AAGTGGTTAGTGCTGTTATT+AGG - chr1_4:19333723-19333742 None:intergenic 35.0%
! CGTTTTCTACATTTAGGTGT+TGG - chr1_4:19333777-19333796 None:intergenic 35.0%
!!! TTATGGTTTTGCTGTTTGAG+TGG - chr1_4:19333823-19333842 None:intergenic 35.0%
! TTTCCATTGAGGTTGTTGTT+GGG - chr1_4:19333848-19333867 None:intergenic 35.0%
! TTTTCCATTGAGGTTGTTGT+TGG - chr1_4:19333849-19333868 None:intergenic 35.0%
!! TGAGTTGAAACGTGTTTTGT+GGG - chr1_4:19333923-19333942 None:intergenic 35.0%
!! TGTTTTTGTTGAGACGGTTA+AGG - chr1_4:19333981-19334000 None:intergenic 35.0%
!! GTTGTGTGTTTTTGTTGAGA+CGG - chr1_4:19333987-19334006 None:intergenic 35.0%
TTTGGATCTGATGTAGTTTC+AGG + chr1_4:19334039-19334058 Msa0154620:CDS 35.0%
CAGTATATGGTGATAGATTC+AGG + chr1_4:19334123-19334142 Msa0154620:CDS 35.0%
GAAGTAGCAGGGTTAAAAAT+AGG - chr1_4:19334207-19334226 None:intergenic 35.0%
CTACTTCTGCTTCGTTTATT+GGG + chr1_4:19334220-19334239 Msa0154620:CDS 35.0%
TACTTCTGCTTCGTTTATTG+GGG + chr1_4:19334221-19334240 Msa0154620:CDS 35.0%
! TGTGGATATCAAGTTGCATA+TGG + chr1_4:19334306-19334325 Msa0154620:CDS 35.0%
! TATCAAGTTGCATATGGTGA+TGG + chr1_4:19334312-19334331 Msa0154620:CDS 35.0%
! ATCAAGTTGCATATGGTGAT+GGG + chr1_4:19334313-19334332 Msa0154620:CDS 35.0%
CACCAAACATAACCGAAAAA+GGG + chr1_4:19334758-19334777 Msa0154620:intron 35.0%
ACCAAACATAACCGAAAAAG+GGG + chr1_4:19334759-19334778 Msa0154620:intron 35.0%
AGAGATAAGACAGTATCCAA+AGG - chr1_4:19335518-19335537 None:intergenic 35.0%
CAGTTAACTGATATAGGAAC+CGG + chr1_4:19335706-19335725 Msa0154620:CDS 35.0%
CGTGTCAAAAATAGAAACTC+TGG - chr1_4:19335839-19335858 None:intergenic 35.0%
!!! GTGTCGTTTTATTTTTCAGG+TGG + chr1_4:19335901-19335920 Msa0154620:CDS 35.0%
ACAGAAAGTTCCAACATCAT+CGG - chr1_4:19335968-19335987 None:intergenic 35.0%
TTGCTGAATATTCCCTATGA+TGG - chr1_4:19336022-19336041 None:intergenic 35.0%
ATAGGGAATATTCAGCAAGA+AGG + chr1_4:19336024-19336043 Msa0154620:CDS 35.0%
! GGAATTCAAGTTTCTATCGA+TGG + chr1_4:19336045-19336064 Msa0154620:CDS 35.0%
GTTTCTATCGATGGAACTAA+TGG + chr1_4:19336054-19336073 Msa0154620:CDS 35.0%
!!! GATGGAACTAATGGTTTTGT+AGG + chr1_4:19336063-19336082 Msa0154620:CDS 35.0%
TGTTTGCTTCAACACACATT+GGG - chr1_4:19336096-19336115 None:intergenic 35.0%
ATGTTTGCTTCAACACACAT+TGG - chr1_4:19336097-19336116 None:intergenic 35.0%
TGTTGGAAAGGAAATGGAAG+TGG - chr1_4:19333740-19333759 None:intergenic 40.0%
! AAGTGGTGTTGGAAAGGAAA+TGG - chr1_4:19333746-19333765 None:intergenic 40.0%
! GAGGTTGTTGTTGGGTATTA+TGG - chr1_4:19333840-19333859 None:intergenic 40.0%
ATACCCAACAACAACCTCAA+TGG + chr1_4:19333842-19333861 Msa0154620:CDS 40.0%
!! GTGAGTTGAAACGTGTTTTG+TGG - chr1_4:19333924-19333943 None:intergenic 40.0%
! TTTTGTTGAGACGGTTAAGG+AGG - chr1_4:19333978-19333997 None:intergenic 40.0%
!! ACAGCAACAGAAGCTAGTTT+TGG + chr1_4:19334021-19334040 Msa0154620:CDS 40.0%
TAAACGAAGCAGAAGTAGCA+GGG - chr1_4:19334218-19334237 None:intergenic 40.0%
ATAAACGAAGCAGAAGTAGC+AGG - chr1_4:19334219-19334238 None:intergenic 40.0%
GCTACTTCTGCTTCGTTTAT+TGG + chr1_4:19334219-19334238 Msa0154620:CDS 40.0%
!! TTGATGATGATGTTGCTTGC+CGG + chr1_4:19334274-19334293 Msa0154620:CDS 40.0%
CTTCACACAGTACATACCTT+TGG - chr1_4:19334346-19334365 None:intergenic 40.0%
GCACCAAACATAACCGAAAA+AGG + chr1_4:19334757-19334776 Msa0154620:intron 40.0%
!!! TCCCCTTTTTCGGTTATGTT+TGG - chr1_4:19334763-19334782 None:intergenic 40.0%
AAGCGTCCCTTTATCAACAT+TGG - chr1_4:19335335-19335354 None:intergenic 40.0%
GCTCTTGAAACCATAACCAT+CGG + chr1_4:19335355-19335374 Msa0154620:CDS 40.0%
CTCTTGAAACCATAACCATC+GGG + chr1_4:19335356-19335375 Msa0154620:CDS 40.0%
GATTGCAGTGTCCTTGATAA+CGG - chr1_4:19335386-19335405 None:intergenic 40.0%
GTTATCAAGGACACTGCAAT+CGG + chr1_4:19335385-19335404 Msa0154620:CDS 40.0%
TTATCAAGGACACTGCAATC+GGG + chr1_4:19335386-19335405 Msa0154620:CDS 40.0%
!! TAGAACGGGTTATGAATGAG+AGG - chr1_4:19335610-19335629 None:intergenic 40.0%
TAGTAGAAGCTAGGGTAGAA+CGG - chr1_4:19335625-19335644 None:intergenic 40.0%
CTTCTACTACGTCTCACTTT+CGG + chr1_4:19335636-19335655 Msa0154620:CDS 40.0%
TTCTACTACGTCTCACTTTC+GGG + chr1_4:19335637-19335656 Msa0154620:CDS 40.0%
TTAACTGATATAGGAACCGG+AGG + chr1_4:19335709-19335728 Msa0154620:CDS 40.0%
AACAGTAGGTAACCTGGTAA+TGG - chr1_4:19335758-19335777 None:intergenic 40.0%
!! AAAGCATTGTAGGCAACAGT+AGG - chr1_4:19335772-19335791 None:intergenic 40.0%
GACACGTGTTATGACTTGAA+CGG + chr1_4:19335853-19335872 Msa0154620:CDS 40.0%
CAAAAAATTCCTGGCTGGAA+AGG - chr1_4:19335938-19335957 None:intergenic 40.0%
!!! AGCCAGGAATTTTTTGATCC+CGG + chr1_4:19335942-19335961 Msa0154620:CDS 40.0%
TTGGTGAAGGGATTTGAGTG+AGG - chr1_4:19333636-19333655 None:intergenic 45.0%
!! TTACTGAGTGTGTTGGTGGT+TGG - chr1_4:19333655-19333674 None:intergenic 45.0%
TCTCTTACTGAGTGTGTTGG+TGG - chr1_4:19333659-19333678 None:intergenic 45.0%
TGCTCTCTTACTGAGTGTGT+TGG - chr1_4:19333662-19333681 None:intergenic 45.0%
! GTGAGAAAGTGGTGTTGGAA+AGG - chr1_4:19333752-19333771 None:intergenic 45.0%
TGGAAGTGAGAAAGTGGTGT+TGG - chr1_4:19333757-19333776 None:intergenic 45.0%
!! AGGTGTTGGAAGTGAGAAAG+TGG - chr1_4:19333763-19333782 None:intergenic 45.0%
!! GTAGTTTCAGGCACTGAAGA+AGG + chr1_4:19334051-19334070 Msa0154620:CDS 45.0%
TTCTGCTTCGTTTATTGGGG+TGG + chr1_4:19334224-19334243 Msa0154620:CDS 45.0%
! ATGATGTTGCTTGCCGGAAA+GGG + chr1_4:19334280-19334299 Msa0154620:CDS 45.0%
TTGAAACCATAACCATCGGG+CGG + chr1_4:19335359-19335378 Msa0154620:CDS 45.0%
TGGAACGAGGGAATGTTTGT+TGG + chr1_4:19335418-19335437 Msa0154620:CDS 45.0%
! GAATGTTTGTTGGAGCAGCT+GGG + chr1_4:19335428-19335447 Msa0154620:CDS 45.0%
AATTGGCCCACGAAAGACAT+AGG - chr1_4:19335475-19335494 None:intergenic 45.0%
TATGTCTTTCGTGGGCCAAT+TGG + chr1_4:19335474-19335493 Msa0154620:CDS 45.0%
ATGTCTTTCGTGGGCCAATT+GGG + chr1_4:19335475-19335494 Msa0154620:CDS 45.0%
GGATACTGTCTTATCTCTCG+AGG + chr1_4:19335520-19335539 Msa0154620:CDS 45.0%
TCATCCGGTTCACTCGAATT+CGG + chr1_4:19335550-19335569 Msa0154620:CDS 45.0%
AGTAGAAGCTAGGGTAGAAC+GGG - chr1_4:19335624-19335643 None:intergenic 45.0%
GTGAGACGTAGTAGAAGCTA+GGG - chr1_4:19335633-19335652 None:intergenic 45.0%
AGTGAGACGTAGTAGAAGCT+AGG - chr1_4:19335634-19335653 None:intergenic 45.0%
!! TGCATCGCGAAAAGCATTGT+AGG - chr1_4:19335782-19335801 None:intergenic 45.0%
AAATCTTGACCTTTCCAGCC+AGG + chr1_4:19335926-19335945 Msa0154620:CDS 45.0%
GGGATCAAAAAATTCCTGGC+TGG - chr1_4:19335943-19335962 None:intergenic 45.0%
AAAGTTCCAACATCATCGGC+CGG - chr1_4:19335964-19335983 None:intergenic 45.0%
! CCTATGATGGAAAGACCAGA+GGG - chr1_4:19336009-19336028 None:intergenic 45.0%
! CCTCTGGTCTTTCCATCATA+GGG + chr1_4:19336007-19336026 Msa0154620:CDS 45.0%
GAGTGAGGACTGAGGAATGA+TGG - chr1_4:19333621-19333640 None:intergenic 50.0%
AGGGATTTGAGTGAGGACTG+AGG - chr1_4:19333629-19333648 None:intergenic 50.0%
!! GTGTGTTGGTGGTTGGTGAA+GGG - chr1_4:19333648-19333667 None:intergenic 50.0%
!! AGTGTGTTGGTGGTTGGTGA+AGG - chr1_4:19333649-19333668 None:intergenic 50.0%
!! TCAGGCACTGAAGAAGGAAG+TGG + chr1_4:19334057-19334076 Msa0154620:CDS 50.0%
! GATGATGTTGCTTGCCGGAA+AGG + chr1_4:19334279-19334298 Msa0154620:CDS 50.0%
GATATCCACACCTCCCTTTC+CGG - chr1_4:19334296-19334315 None:intergenic 50.0%
! GGAATGTTTGTTGGAGCAGC+TGG + chr1_4:19335427-19335446 Msa0154620:CDS 50.0%
TGTCTTATCTCTCGAGGCAC+TGG + chr1_4:19335526-19335545 Msa0154620:CDS 50.0%
AATGCCCGTAGGTGCAATGT+GGG + chr1_4:19335582-19335601 Msa0154620:CDS 50.0%
AGGAACCCACATTGCACCTA+CGG - chr1_4:19335590-19335609 None:intergenic 50.0%
! TCACTTTCGGGTCTTGCAGT+TGG + chr1_4:19335649-19335668 Msa0154620:CDS 50.0%
!! TAACCTGGTAATGGCTGTGC+CGG - chr1_4:19335749-19335768 None:intergenic 50.0%
GTAGGCAACAGTAGGTAACC+TGG - chr1_4:19335764-19335783 None:intergenic 50.0%
! ATAGAAACTCTGGGAGCACG+TGG - chr1_4:19335829-19335848 None:intergenic 50.0%
CGACACTGTTGGAACTCGAA+CGG - chr1_4:19335887-19335906 None:intergenic 50.0%
GGCCGGGATCAAAAAATTCC+TGG - chr1_4:19335947-19335966 None:intergenic 50.0%
AAGTTCCAACATCATCGGCC+GGG - chr1_4:19335963-19335982 None:intergenic 50.0%
ATGGAAAGACCAGAGGGTGA+AGG - chr1_4:19336003-19336022 None:intergenic 50.0%
! CCCTCTGGTCTTTCCATCAT+AGG + chr1_4:19336006-19336025 Msa0154620:CDS 50.0%
! CCCTATGATGGAAAGACCAG+AGG - chr1_4:19336010-19336029 None:intergenic 50.0%
! ATGTTGCTTGCCGGAAAGGG+AGG + chr1_4:19334283-19334302 Msa0154620:CDS 55.0%
!! GGTGATGGGTCGTCTACCAA+AGG + chr1_4:19334327-19334346 Msa0154620:CDS 55.0%
AAGGACACTGCAATCGGGTG+CGG + chr1_4:19335391-19335410 Msa0154620:CDS 55.0%
GTTGGAGCAGCTGGGTTGTT+AGG + chr1_4:19335436-19335455 Msa0154620:CDS 55.0%
AAGACATAGGTCCGCCTCCA+AGG - chr1_4:19335462-19335481 None:intergenic 55.0%
AGGCGGACCTATGTCTTTCG+TGG + chr1_4:19335465-19335484 Msa0154620:CDS 55.0%
GGCGGACCTATGTCTTTCGT+GGG + chr1_4:19335466-19335485 Msa0154620:CDS 55.0%
ACAGTATCCAAAGGCTCCGC+CGG - chr1_4:19335509-19335528 None:intergenic 55.0%
!! TCTCGAGGCACTGGATCATC+CGG + chr1_4:19335535-19335554 Msa0154620:CDS 55.0%
GCGTCCGAATTCGAGTGAAC+CGG - chr1_4:19335557-19335576 None:intergenic 55.0%
! TTGCACCTACGGGCATTGCT+TGG - chr1_4:19335579-19335598 None:intergenic 55.0%
CAATGCCCGTAGGTGCAATG+TGG + chr1_4:19335581-19335600 Msa0154620:CDS 55.0%
GGAACCCACATTGCACCTAC+GGG - chr1_4:19335589-19335608 None:intergenic 55.0%
! CTTTCGGGTCTTGCAGTTGG+AGG + chr1_4:19335652-19335671 Msa0154620:CDS 55.0%
AGGAACCGGAGGAGTTGTCA+TGG + chr1_4:19335720-19335739 Msa0154620:CDS 55.0%
GGTGTCCATGACAACTCCTC+CGG - chr1_4:19335728-19335747 None:intergenic 55.0%
GGAGGAGTTGTCATGGACAC+CGG + chr1_4:19335727-19335746 Msa0154620:CDS 55.0%
TTGATCCCGGCCGATGATGT+TGG + chr1_4:19335955-19335974 Msa0154620:CDS 55.0%
GCATTTGCTCCTTCACCCTC+TGG + chr1_4:19335991-19336010 Msa0154620:CDS 55.0%
AACGGTCCGCCCGATGGTTA+TGG - chr1_4:19335368-19335387 None:intergenic 60.0%
!! CTTGATAACGGTCCGCCCGA+TGG - chr1_4:19335374-19335393 None:intergenic 60.0%
CATCGGGCGGACCGTTATCA+AGG + chr1_4:19335372-19335391 Msa0154620:CDS 60.0%
CTGCAATCGGGTGCGGACAT+TGG + chr1_4:19335398-19335417 Msa0154620:CDS 60.0%
GGGTGCGGACATTGGAACGA+GGG + chr1_4:19335406-19335425 Msa0154620:CDS 60.0%
GCAGCTGGGTTGTTAGGCCT+TGG + chr1_4:19335442-19335461 Msa0154620:CDS 60.0%
GCTGGGTTGTTAGGCCTTGG+AGG + chr1_4:19335445-19335464 Msa0154620:CDS 60.0%
GGGTTGTTAGGCCTTGGAGG+CGG + chr1_4:19335448-19335467 Msa0154620:CDS 60.0%
GGCCAATTGGGAGCCCAAAC+CGG + chr1_4:19335487-19335506 Msa0154620:CDS 60.0%
CAATTGGGAGCCCAAACCGG+CGG + chr1_4:19335490-19335509 Msa0154620:CDS 60.0%
!! TCCAAAGGCTCCGCCGGTTT+GGG - chr1_4:19335503-19335522 None:intergenic 60.0%
! ATCCAAAGGCTCCGCCGGTT+TGG - chr1_4:19335504-19335523 None:intergenic 60.0%
ACACCGGCACAGCCATTACC+AGG + chr1_4:19335743-19335762 Msa0154620:CDS 60.0%
GCTTGCCGGAAAGGGAGGTG+TGG + chr1_4:19334288-19334307 Msa0154620:CDS 65.0%
CGGGTGCGGACATTGGAACG+AGG + chr1_4:19335405-19335424 Msa0154620:CDS 65.0%
!! CGCCGGTTTGGGCTCCCAAT+TGG - chr1_4:19335492-19335511 None:intergenic 65.0%
GGACGCCAAGCAATGCCCGT+AGG + chr1_4:19335571-19335590 Msa0154620:CDS 65.0%
GCCCAAACCGGCGGAGCCTT+TGG + chr1_4:19335499-19335518 Msa0154620:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 19333555 19336245 19333555 ID=Msa0154620;Name=Msa0154620
chr1_4 mRNA 19333555 19336245 19333555 ID=Msa0154620-mRNA-1;Parent=Msa0154620;Name=Msa0154620-mRNA-1;_AED=0.02;_eAED=0.02;_QI=133|1|1|1|1|1|2|138|473
chr1_4 exon 19333555 19334348 19333555 ID=Msa0154620-mRNA-1:exon:7105;Parent=Msa0154620-mRNA-1
chr1_4 exon 19335347 19336245 19335347 ID=Msa0154620-mRNA-1:exon:7106;Parent=Msa0154620-mRNA-1
chr1_4 five_prime_UTR 19333555 19333687 19333555 ID=Msa0154620-mRNA-1:five_prime_utr;Parent=Msa0154620-mRNA-1
chr1_4 CDS 19333688 19334348 19333688 ID=Msa0154620-mRNA-1:cds;Parent=Msa0154620-mRNA-1
chr1_4 CDS 19335347 19336107 19335347 ID=Msa0154620-mRNA-1:cds;Parent=Msa0154620-mRNA-1
chr1_4 three_prime_UTR 19336108 19336245 19336108 ID=Msa0154620-mRNA-1:three_prime_utr;Parent=Msa0154620-mRNA-1
Gene Sequence

>Msa0154620

ATGCTGCTTCAGTTCCCTCTTCTACTCCTAATCCTAATAACAGCACTAACCACTTCCATTTCCTTTCCAACACCACTTTCTCACTTCCAACACCTAAATGTAGAAAACGCAATCTCAGAAACAAAACTAAAACCACTCAAACAGCAAAACCATAATACCCAACAACAACCTCAATGGAAAACAAAACTGTTTCACAGAGACAACATAAACCTTAAAAACACCAAAAAAACAACCCACAAAACACGTTTCAACTCACGTATCAACAGAGACATAAAAAGAGTAACATTCCTCCTTAACCGTCTCAACAAAAACACACAACAACAAGAAACAACAACAGCAACAGAAGCTAGTTTTGGATCTGATGTAGTTTCAGGCACTGAAGAAGGAAGTGGTGAATATTTTGTTAGAATAGGAATTGGTAGTCCTGCTATTTATCAGTATATGGTGATAGATTCAGGAAGTGATATTGTTTGGATTCAATGTGAACCTTGTGATCAATGTTATAATCAAACTGATCCTATTTTTAACCCTGCTACTTCTGCTTCGTTTATTGGGGTGGCTTGTTCTTCTAATGTTTGTAATCAGCTTGATGATGATGTTGCTTGCCGGAAAGGGAGGTGTGGATATCAAGTTGCATATGGTGATGGGTCGTCTACCAAAGGGACGCTTGCTCTTGAAACCATAACCATCGGGCGGACCGTTATCAAGGACACTGCAATCGGGTGCGGACATTGGAACGAGGGAATGTTTGTTGGAGCAGCTGGGTTGTTAGGCCTTGGAGGCGGACCTATGTCTTTCGTGGGCCAATTGGGAGCCCAAACCGGCGGAGCCTTTGGATACTGTCTTATCTCTCGAGGCACTGGATCATCCGGTTCACTCGAATTCGGACGCCAAGCAATGCCCGTAGGTGCAATGTGGGTTCCTCTCATTCATAACCCGTTCTACCCTAGCTTCTACTACGTCTCACTTTCGGGTCTTGCAGTTGGAGGCATTCGAGTCCCCATATCCGAACAAATATTTCAGTTAACTGATATAGGAACCGGAGGAGTTGTCATGGACACCGGCACAGCCATTACCAGGTTACCTACTGTTGCCTACAATGCTTTTCGCGATGCATTTATTGCTCAGACAACAAATTTGCCACGTGCTCCCAGAGTTTCTATTTTTGACACGTGTTATGACTTGAACGGTTTTGTTACCGTTCGAGTTCCAACAGTGTCGTTTTATTTTTCAGGTGGTCAAATCTTGACCTTTCCAGCCAGGAATTTTTTGATCCCGGCCGATGATGTTGGAACTTTCTGTTTTGCATTTGCTCCTTCACCCTCTGGTCTTTCCATCATAGGGAATATTCAGCAAGAAGGAATTCAAGTTTCTATCGATGGAACTAATGGTTTTGTAGGATTTGGACCCAATGTGTGTTGA
Protein sequence

>Msa0154620

MLLQFPLLLLILITALTTSISFPTPLSHFQHLNVENAISETKLKPLKQQNHNTQQQPQWKTKLFHRDNINLKNTKKTTHKTRFNSRINRDIKRVTFLLNRLNKNTQQQETTTATEASFGSDVVSGTEEGSGEYFVRIGIGSPAIYQYMVIDSGSDIVWIQCEPCDQCYNQTDPIFNPATSASFIGVACSSNVCNQLDDDVACRKGRCGYQVAYGDGSSTKGTLALETITIGRTVIKDTAIGCGHWNEGMFVGAAGLLGLGGGPMSFVGQLGAQTGGAFGYCLISRGTGSSGSLEFGRQAMPVGAMWVPLIHNPFYPSFYYVSLSGLAVGGIRVPISEQIFQLTDIGTGGVVMDTGTAITRLPTVAYNAFRDAFIAQTTNLPRAPRVSIFDTCYDLNGFVTVRVPTVSFYFSGGQILTFPARNFLIPADDVGTFCFAFAPSPSGLSIIGNIQQEGIQVSIDGTNGFVGFGPNVC*