AlfalfaGEDB | Msa0535210

M. sativa cultivar zhongmu-4 / Msa0535210

KEGG: -
GO: -
PFAM: PF01582.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535210	AES87048.2	46.429	196	89	2	672	867	6	185	4.72e-37	166
Msa0535210	AES87048.2	66.990	206	51	2	173	378	6	194	2.91e-68	268
Msa0535210	AES87048.2	74.426	1611	280	14	8	1572	190	1714	0.0	2345

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535210	sp\|V9M398\|RUN1_VITRO	39.649	797	418	12	524	1283	27	797	5.32e-157	516
Msa0535210	sp\|V9M398\|RUN1_VITRO	34.325	504	302	13	16	510	27	510	1.53e-61	236

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535210	G7JSA9	46.429	196	89	2	672	867	6	185	2.25e-37	166
Msa0535210	G7JSA9	66.990	206	51	2	173	378	6	194	1.39e-68	268
Msa0535210	G7JSA9	74.426	1611	280	14	8	1572	190	1714	0.0	2345

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007080	Msa0535210	0.810044	1.401589e-50	-8.615850e-47
Msa0039330	Msa0535210	0.816737	4.738678e-52	-8.615850e-47
Msa0101810	Msa0535210	-0.810002	1.431314e-50	-8.615850e-47
Msa0188830	Msa0535210	0.801864	7.363792e-49	-8.615850e-47
Msa0195770	Msa0535210	0.811178	7.972946e-51	-8.615850e-47
Msa0195820	Msa0535210	0.804452	2.145536e-49	-8.615850e-47
Msa0212310	Msa0535210	0.815345	9.694184e-52	-8.615850e-47
Msa0217400	Msa0535210	0.812060	5.126684e-51	-8.615850e-47
Msa0221060	Msa0535210	0.810827	9.498978e-51	-8.615850e-47
Msa0260810	Msa0535210	0.800217	1.598769e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535210	MtrunA17_Chr4g0009141	70.665	1609	394	15	1	1557	1	1583	0.0	2218
Msa0535210	MtrunA17_Chr4g0009101	69.951	1624	405	16	7	1570	22	1622	0.0	2174
Msa0535210	MtrunA17_Chr4g0009241	69.106	1599	390	13	1	1532	1	1562	0.0	2130
Msa0535210	MtrunA17_Chr4g0009301	68.372	1603	411	19	1	1532	1	1578	0.0	2110
Msa0535210	MtrunA17_Chr4g0009041	67.875	1600	392	18	1	1532	1	1546	0.0	2045
Msa0535210	MtrunA17_Chr4g0009401	66.242	1570	443	13	16	1532	5	1540	0.0	1997
Msa0535210	MtrunA17_Chr4g0009401	41.211	512	270	13	14	514	503	994	1.38e-86	313
Msa0535210	MtrunA17_Chr4g0009171	73.644	1051	222	6	521	1532	729	1763	0.0	1521
Msa0535210	MtrunA17_Chr4g0009171	76.984	756	158	5	518	1273	1	740	0.0	1167
Msa0535210	MtrunA17_Chr4g0009171	40.643	529	272	12	11	523	2	504	1.01e-93	336

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535210	AT5G36930.3	42.466	803	426	10	524	1321	14	785	1.55e-178	566
Msa0535210	AT5G36930.3	33.463	514	310	11	13	517	11	501	9.68e-56	213
Msa0535210	AT5G36930.4	42.466	803	426	10	524	1321	14	785	7.57e-178	566
Msa0535210	AT5G36930.4	33.592	515	308	13	13	517	11	501	1.05e-55	213
Msa0535210	AT5G36930.1	42.466	803	426	10	524	1321	14	785	7.57e-178	566
Msa0535210	AT5G36930.1	33.592	515	308	13	13	517	11	501	1.05e-55	213
Msa0535210	AT5G36930.2	42.466	803	426	10	524	1321	17	788	9.14e-178	566
Msa0535210	AT5G36930.2	33.592	515	308	13	13	517	14	504	1.06e-55	213
Msa0535210	AT1G27170.1	35.750	800	452	9	524	1275	14	799	3.39e-144	479
Msa0535210	AT1G27170.1	30.534	524	324	17	8	514	6	506	3.00e-50	196

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Find 291 sgRNAs with CRISPR-Local

Find 536 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCCCTTATATTCAAA+AGG	0.436995	4_1:+11302362	None:intergenic
AAAGAATGCACTTGAGTGGA+AGG	0.588143	4_1:-11305000	Msa0535210:CDS
AAAGAATTCGAAGCTTTAGA+AGG	0.424141	4_1:+11300327	None:intergenic
AAAGCCATTTACAATCAAAT+TGG	0.276057	4_1:-11302409	Msa0535210:CDS
AAAGCTTCGAATTCTTTGTG+TGG	0.606680	4_1:-11300321	Msa0535210:CDS
AAAGGCTTTGTGTGGAAATC+GGG	0.407627	4_1:-11305270	Msa0535210:CDS
AACACTGCAATCCAGTAGAC+GGG	0.519634	4_1:-11299334	Msa0535210:CDS
AACAGAAGCTGTCAAGGGAC+TGG	0.543672	4_1:-11301290	Msa0535210:CDS
AACAGAGTGGCAGAAAGTTT+TGG	0.303211	4_1:-11301888	Msa0535210:CDS
AACATTCAGAGTTGAATCTG+AGG	0.594524	4_1:+11306200	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGGTTTGAAAGTGAAGAT+CGG	-	chr4_1:11299454-11299473	Msa0535210:CDS	30.0%
GATTCAACTCTGAATGTTAT+TGG	-	chr4_1:11299495-11299514	Msa0535210:CDS	30.0%
AAGATGGTGTCTTAAAGAAT+TGG	-	chr4_1:11299566-11299585	Msa0535210:intron	30.0%
ATTCATCCAACAAAAGATTG+TGG	-	chr4_1:11299649-11299668	Msa0535210:intron	30.0%
TTCATCCAACAAAAGATTGT+GGG	-	chr4_1:11299650-11299669	Msa0535210:intron	30.0%
CTTGTCTTTATCTTCAGAAT+AGG	+	chr4_1:11299727-11299746	None:intergenic	30.0%
TCTAATGATAAGCAAGAAGA+CGG	-	chr4_1:11300030-11300049	Msa0535210:intron	30.0%
CAATAGTTGAATCACATCTT+GGG	+	chr4_1:11300089-11300108	None:intergenic	30.0%
TCAATAGTTGAATCACATCT+TGG	+	chr4_1:11300090-11300109	None:intergenic	30.0%
TCAAAATCTCCTCTTCTAAT+AGG	-	chr4_1:11300115-11300134	Msa0535210:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	11299151	11306561	11299151	ID=Msa0535210;Name=Msa0535210
chr4_1	mRNA	11299151	11306561	11299151	ID=Msa0535210-mRNA-1;Parent=Msa0535210;Name=Msa0535210-mRNA-1;_AED=0.07;_eAED=0.07;_QI=152\|0.85\|0.75\|1\|1\|1\|8\|0\|1572
chr4_1	exon	11299151	11299578	11299151	ID=Msa0535210-mRNA-1:exon:3627;Parent=Msa0535210-mRNA-1
chr4_1	exon	11299680	11299743	11299680	ID=Msa0535210-mRNA-1:exon:3626;Parent=Msa0535210-mRNA-1
chr4_1	exon	11300064	11300861	11300064	ID=Msa0535210-mRNA-1:exon:3625;Parent=Msa0535210-mRNA-1
chr4_1	exon	11301039	11301314	11301039	ID=Msa0535210-mRNA-1:exon:3624;Parent=Msa0535210-mRNA-1
chr4_1	exon	11301529	11302666	11301529	ID=Msa0535210-mRNA-1:exon:3623;Parent=Msa0535210-mRNA-1
chr4_1	exon	11303960	11304414	11303960	ID=Msa0535210-mRNA-1:exon:3622;Parent=Msa0535210-mRNA-1
chr4_1	exon	11304683	11305724	11304683	ID=Msa0535210-mRNA-1:exon:3621;Parent=Msa0535210-mRNA-1
chr4_1	exon	11305892	11306561	11305892	ID=Msa0535210-mRNA-1:exon:3620;Parent=Msa0535210-mRNA-1
chr4_1	five_prime_UTR	11306410	11306561	11306410	ID=Msa0535210-mRNA-1:five_prime_utr;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11305892	11306409	11305892	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11304683	11305724	11304683	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11303960	11304414	11303960	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11301529	11302666	11301529	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11301039	11301314	11301039	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11300064	11300861	11300064	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11299680	11299743	11299680	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1
chr4_1	CDS	11299151	11299578	11299151	ID=Msa0535210-mRNA-1:cds;Parent=Msa0535210-mRNA-1

Gene Sequence

>Msa0535210

ATGTCTTCCTTCACAACTTCCGCTACTGATTCAAACAGGAAGAGGTACGATGTGTACCTGAGTTTCTGTGACGAAGACTCGCGTTTTTTTGTTTTGGGTATATATACAGCTTTTACTTCAAAACCTGACGTTGTTGTTTTCTGGGAGGATCAATGGTTTGAAAGTGAAGATCGGAGATCAGAACAACCCTCAGATTCAACTCTGAATGTTATTGGAGATTGCAAAATAGTTGTAATTGTATTTTCAAAGAATTATTTCAATTCAAGATGGTGTCTTAAAGAATTGGAGAAAATAATCCAGTGTTGCCATAGAGCCACGGATGGTCTCATTGTTCTGCCTGTGTTCTATTCATCCAACAAAAGATTGTGGGTACAAAGTGATATGTATGAAGAATTGGACAAAATTTCGATGCAGGAGGAGACCTATTCTGAAGATAAAGACAAGTTCATGACTTGTGTGGCAGCAATCACTAATGAAGCTTCCAAATATGCTTTTGATTCTTTACATTGCGAACATGAGAACAGAGGCGAACATATAACACATGTAGTGAAATGTGCTACTCTAATGATAAGCAAGAAGACGGGCCCGTTCCATATAGAGAGCGTACATTCTCGTGCCCAAGATGTGATTCAACTATTGAAACAATCAAAATCTCCTCTTCTAATAGGGATATGGGGGATGAAAGGGATTGGTAAATCAACCATTGCCCAATCTATTTATTATCAAGGTTGTCCCTATTTTGAGCACAAGTGCTTCCTCAGAAACATACGGGGAGCTTGGGAAGAAGATAATGGTCAAGTTTCTTTACAAGATAAACTTCTTTTTTATATTGACGGAGCAACTGAAATAAAGATACCTACAGTTGAATCAGGAAGAGTTCTTTTAAAAGAAAGACTTCGACATAAAAGGGTACTTCTTCTACTTGACAGTGTTGATAAATTGGAGCAACTAAAGGCTTTGTGTGGAAATCGGGATTGGTTTGGCCCAGGCAGCAAAATAATCATCACAACAAGCAATAGACATCTGCTCAAGGAGCATGGAGTTGACCATATATATAGAATGAAAGAACTAGACGAAAGTGAATCACTTATGGTTTTGAGTCAGGGTGCATTCAAGCAAGCAGCTTCTCCCGAAGCTTTTACTGAACCTTCCAAACAGATAGTTGCTTATTGTGAGGGATTGCCTCTTGCTCTTGAAGTCTTTGGCAGGGATTTGTGTGGAAAGAATGCACTTGAGTGGAAGGGTGTGTTGAGGAGTCTTGAAAGATTTTCCATTCCAGCTCCACGTCTACTAGACGCTCTAGAAAAGAGTTTGAGTGATTTGGGCGATGAAGAGAAACAAATATTCTTTGATATAGCATGTTTCTTTATCGGGATGAGCCTAAATGATGTACTTCAAACATTAAATAGGTCAATACAATGCACAACTCTTCAAATAAATTGCCTTGAAGACAAAAGCTTTGTAACCATTGATGAGAATAACAAACTTCAAATGCATGTTCTGCTACAGGCCATGGCAAGAGATATCATTAACAGAGAGTCAAGTAATAAGATGTATCAGCCAAAGATGTACGACGTGTTCTTGAGTTTCAGAGGAGAAGATTGTCGTACAAAGTTCATATCACATCTTTATACCTCTCTTCAAAATGCTGGAATTTATACTTTCAGAGATGATGACGAGATTCAGAGAGGAGATCAAATCTCAATCTCACTGTTACAAGCAATCGGACGGTCTAGAATTTCTATTGTTGTTTTGTCTACAACTTATGCCAATTCAAGATGGTGTATGCTAGAGTTGGAGAAGATAATGGAAATTGGCAGAACTAGGGATCTGATCGTTGTGCCAGTGTTCTACGAGGTAGATCCCTCAGAAGTACGTCATCAGACAGGACAGTTTGGAGTTTGCTTTGAAGATCTTATATCAAAAATCTCAGTGGGCGAGTCTACCAAGAGGAATTGGAGAAGAGACCTCATTGATATTGGTGGCATAGCAGGATTTGTACTCATTGATTCCAGGAATGAAAGTGCAGATATCAAGAATGTTGTTGAACGAGTCACCCGTTTGCTAGATAGGACAGAGTTATTTGTTGCCGAACATCCGGTGGGTGTAGAATCTCGTGTGGAAGCTGCAACTAAACTATTAAAGATCCCAAAATCTTTGTACCCAAAAAAAAAAAAGATCCCAAAATCAAAAGATGTTTTGCTTCTAGGGATATGGGGAATGGGGGGTACGGGTAAAACAACCATTGCCAAAGCCATTTACAATCAAATTGGAAGCAAATTTGAAGGAAAGAGCTTCCTTTTGAATATAAGGGAATTTTGGGAGGCAAATATTAATCTAGTTTCTCTACAGCAACAAGTTCTTTGTGATGTTTACAAAACAACAACATTCAAGATACGCGACATCGATTCAGGGAAAAATATATTGAAGGAAAAGCTTGCCCAGAATAGGGTACTTTTTGTGCTTGATGATGTGAATGATTTGGACCAACTAAAAGCTTTATGTGGAAGCCGCGAATGGTTTGGTCCGGGGAGTAGAATAATAATCACAACAAGAGATATGCATCTTCTTAGATCGTGTAGAGTAGATGAAGTGTATGCAATACAAGAAATGGACGAAAGTGAATCTCTTGAGCTTTTCAGTTGGCATGCATTCAAGCAACCAAGTCCGGCAGAAGGTTTTACTACACATTCAACAGATGTGATTGCTTATTCTGGGAGATTGCCACTGGCACTTGAAGTCCTTGGGTCATATTTGTCTGACTGTGAAATAACAGAGTGGCAGAAAGTTTTGGAGAAACTCAAATGTATTCCCCATCATGAAGTACAAAAGAAGTTGAAAGTAAGTTTTGATGGTTTAAAAGATGTTACAGAGCAACAAATCTTCCTTGATATAGCTTGTTTCTTTATTGGGATGGACCGGAATGATACCATACAAATATTAAATGGGTGTGGATTTTTTGCTGATATTGGAATTAAAGTCCTTGTAGAGCGAAGCCTTGTAACTATTGACAATAGGAACAAGCTTAGAATGCATGATTTGTTGAGAGATATGGGAAGGCAAATAATTTACGAGGAGTCACCTTTAGATCCGGAGAAGCGCAGTAGGTTGTGGCGGTGTGAGGAAGTGTTTGATATATTATCAAAGCATAAGGGAACAGAAGCTGTCAAGGGACTGGCATTGGAGTTTCCAAGGAAGAATACAGTTTCTCTGAATACCAAAGCATTTAAAAAGATGAATAAGCTCAGATTGCTTCAACTTGCCGGAGTGAAGCTTAAGGGAGATTTTAAATATCTTTCCGGAGATCTTAAGTTTCTATACTGGCATGAATTTCCAAAAACCTACATTCCTACAGAATTTCAACAAGGAAGCCTAGTTGCTATCACATTAAAATACAGTAATCTAAAACAAATATCGAAGAAGAGCCAGATGCTAGAGAACCTAAAAATTCTTAATCTCAGTTATTCTCAGAATTTGACTGAAACCCCTGACTTTTCATGCTTGCCTAATCTTGAAAAGCTAGTGCTCAAAGACTGTCCAAGTTTGTCTACAGTCTCCCATAGCATTGGATCCCTCCATAAACTTCTTATGATAAATTTGACAAACTGCACTGGTCTTCGAAAACTTCCAAGAAGCATCTATAAATTAAAATCTCTAGAAACTTTGATTCTTTCAGGATGTTCCAAGATTGACAAATTGGAGGAGGACTTGGAACAGATGGTATCATTGAAAACCTTGATTGCAGATAAGACTGCAATAACAAAAGTTCCCTTTTCAATTGTAAGATCAAAAAGCATCGGATTTATTTCTTTATGTGGCTTTAAAGGATTTACACGTGATGTATTTCCTTCTCTCATTCGGTCTTGGTTGTCACCGTCAAATAATGTTATATCCCGAGTTCAGACATCGATGTCCCTGTCATCTCTTGGTACTTTCAAGGACCTTCTAAAGCTTCGAATTCTTTGTGTGGAGTGTGGATCACAACTACAACTAAATCAAGATATAACAAGAATTTTGGATGCATTAAAAGGCAAAAAATGTCATGAATGGGAAGCAAGTGCAAGTTCAACTACATCACAAATCTCTGATATGTATGATTCTCCTTTAATTGATGATTGCCTTCCTCAAGTTCACATTTCAAGGTCAAACAATTACTCGAAATCCCTTTTAATTCAAATGGGAACGAAATGTCAAGTTTCAAATATTACCGAAGACGGTATTTTCCAGACTGCGAATGGGACTTGTGGTTCGTTTTTCCTCTCCTCTGATAATAACTCCTTCAGTTGCAAAGAAGGTTGCCAAGGTGTTTTGATCATTAATCACACAAAGAACACCATTCAACTCTATAAAAGAGAAACACTAGCCTCTTTTGGCCATGGGGATTGGCAGAGTATAACATCAAATCTAGAACCTGGTAACAAAGTGGAGGTTATTGTTGTTTTTGGGGAGGGATTCATTGTAGAAAAGACAACATTATCTCTTTTATATGATGAACCAATCAACAAAGAAATTGAACACTGCAATCCAGTAGACGGGGAAGATGTTATTGTTTCTGCTCATGATGATAAGAATGTTGGTGTTTCTGGTGGTGATAATATCGATATGCCGGTGGACAACAATGTTACGGGTCAAGAGCAAGATGAAAATATAAGTGAAGATGAGCACAAACATGAAGAAACGTGTAGAAAAAGAAAGAGAGGAGACATGACAGAACTGTAA

Protein sequence

>Msa0535210

MSSFTTSATDSNRKRYDVYLSFCDEDSRFFVLGIYTAFTSKPDVVVFWEDQWFESEDRRSEQPSDSTLNVIGDCKIVVIVFSKNYFNSRWCLKELEKIIQCCHRATDGLIVLPVFYSSNKRLWVQSDMYEELDKISMQEETYSEDKDKFMTCVAAITNEASKYAFDSLHCEHENRGEHITHVVKCATLMISKKTGPFHIESVHSRAQDVIQLLKQSKSPLLIGIWGMKGIGKSTIAQSIYYQGCPYFEHKCFLRNIRGAWEEDNGQVSLQDKLLFYIDGATEIKIPTVESGRVLLKERLRHKRVLLLLDSVDKLEQLKALCGNRDWFGPGSKIIITTSNRHLLKEHGVDHIYRMKELDESESLMVLSQGAFKQAASPEAFTEPSKQIVAYCEGLPLALEVFGRDLCGKNALEWKGVLRSLERFSIPAPRLLDALEKSLSDLGDEEKQIFFDIACFFIGMSLNDVLQTLNRSIQCTTLQINCLEDKSFVTIDENNKLQMHVLLQAMARDIINRESSNKMYQPKMYDVFLSFRGEDCRTKFISHLYTSLQNAGIYTFRDDDEIQRGDQISISLLQAIGRSRISIVVLSTTYANSRWCMLELEKIMEIGRTRDLIVVPVFYEVDPSEVRHQTGQFGVCFEDLISKISVGESTKRNWRRDLIDIGGIAGFVLIDSRNESADIKNVVERVTRLLDRTELFVAEHPVGVESRVEAATKLLKIPKSLYPKKKKIPKSKDVLLLGIWGMGGTGKTTIAKAIYNQIGSKFEGKSFLLNIREFWEANINLVSLQQQVLCDVYKTTTFKIRDIDSGKNILKEKLAQNRVLFVLDDVNDLDQLKALCGSREWFGPGSRIIITTRDMHLLRSCRVDEVYAIQEMDESESLELFSWHAFKQPSPAEGFTTHSTDVIAYSGRLPLALEVLGSYLSDCEITEWQKVLEKLKCIPHHEVQKKLKVSFDGLKDVTEQQIFLDIACFFIGMDRNDTIQILNGCGFFADIGIKVLVERSLVTIDNRNKLRMHDLLRDMGRQIIYEESPLDPEKRSRLWRCEEVFDILSKHKGTEAVKGLALEFPRKNTVSLNTKAFKKMNKLRLLQLAGVKLKGDFKYLSGDLKFLYWHEFPKTYIPTEFQQGSLVAITLKYSNLKQISKKSQMLENLKILNLSYSQNLTETPDFSCLPNLEKLVLKDCPSLSTVSHSIGSLHKLLMINLTNCTGLRKLPRSIYKLKSLETLILSGCSKIDKLEEDLEQMVSLKTLIADKTAITKVPFSIVRSKSIGFISLCGFKGFTRDVFPSLIRSWLSPSNNVISRVQTSMSLSSLGTFKDLLKLRILCVECGSQLQLNQDITRILDALKGKKCHEWEASASSTTSQISDMYDSPLIDDCLPQVHISRSNNYSKSLLIQMGTKCQVSNITEDGIFQTANGTCGSFFLSSDNNSFSCKEGCQGVLIINHTKNTIQLYKRETLASFGHGDWQSITSNLEPGNKVEVIVVFGEGFIVEKTTLSLLYDEPINKEIEHCNPVDGEDVIVSAHDDKNVGVSGGDNIDMPVDNNVTGQEQDENISEDEHKHEETCRKRKRGDMTEL*