Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703680 | XP_013446789.2 | 100.000 | 309 | 0 | 0 | 1 | 309 | 37 | 345 | 0.0 | 619 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703680 | sp|Q9FYE5|UUAT1_ARATH | 78.964 | 309 | 65 | 0 | 1 | 309 | 1 | 309 | 0.0 | 505 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703680 | A0A072TUH1 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 619 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004540 | Msa0703680 | 0.809964 | 1.458404e-50 | -8.615850e-47 |
| Msa0016670 | Msa0703680 | 0.816152 | 6.409116e-52 | -8.615850e-47 |
| Msa0057710 | Msa0703680 | 0.818444 | 1.953733e-52 | -8.615850e-47 |
| Msa0073670 | Msa0703680 | 0.809196 | 2.132241e-50 | -8.615850e-47 |
| Msa0093850 | Msa0703680 | 0.805618 | 1.223990e-49 | -8.615850e-47 |
| Msa0614880 | Msa0703680 | 0.919893 | 2.546523e-87 | -8.615850e-47 |
| Msa0614930 | Msa0703680 | 0.970594 | 7.444186e-132 | -8.615850e-47 |
| Msa0615420 | Msa0703680 | 0.826477 | 2.655785e-54 | -8.615850e-47 |
| Msa0642880 | Msa0703680 | 0.815606 | 8.482827e-52 | -8.615850e-47 |
| Msa0698640 | Msa0703680 | 0.812322 | 4.493567e-51 | -8.615850e-47 |
| Msa0702340 | Msa0703680 | 0.854537 | 1.152014e-61 | -8.615850e-47 |
| Msa0702590 | Msa0703680 | 0.853520 | 2.262908e-61 | -8.615850e-47 |
| Msa0702660 | Msa0703680 | 0.857482 | 1.584002e-62 | -8.615850e-47 |
| Msa0703680 | Msa0716410 | 0.800924 | 1.147295e-48 | -8.615850e-47 |
| Msa0703680 | Msa0788770 | 0.828796 | 7.367411e-55 | -8.615850e-47 |
| Msa0703680 | Msa0799000 | 0.821683 | 3.543782e-53 | -8.615850e-47 |
| Msa0703680 | Msa0807820 | 0.800454 | 1.430575e-48 | -8.615850e-47 |
| Msa0703680 | Msa0824640 | 0.802094 | 6.603768e-49 | -8.615850e-47 |
| Msa0703680 | Msa0861510 | 0.818261 | 2.150106e-52 | -8.615850e-47 |
| Msa0703680 | Msa0861530 | 0.815937 | 7.156958e-52 | -8.615850e-47 |
| Msa0703680 | Msa0861600 | 0.802173 | 6.361275e-49 | -8.615850e-47 |
| Msa0703680 | Msa0864010 | 0.802391 | 5.735226e-49 | -8.615850e-47 |
| Msa0703680 | Msa0930780 | 0.829664 | 4.534867e-55 | -8.615850e-47 |
| Msa0703680 | Msa1050910 | 0.815742 | 7.911410e-52 | -8.615850e-47 |
| Msa0703680 | Msa1050960 | 0.817225 | 3.682982e-52 | -8.615850e-47 |
| Msa0703680 | Msa1055010 | 0.800793 | 1.220143e-48 | -8.615850e-47 |
| Msa0703680 | Msa1079230 | 0.803550 | 3.303787e-49 | -8.615850e-47 |
| Msa0703680 | Msa1097820 | 0.802250 | 6.133413e-49 | -8.615850e-47 |
| Msa0703680 | Msa1124110 | 0.822198 | 2.693194e-53 | -8.615850e-47 |
| Msa0703680 | Msa1168950 | 0.819542 | 1.099515e-52 | -8.615850e-47 |
| Msa0703680 | Msa1169040 | 0.815130 | 1.082104e-51 | -8.615850e-47 |
| Msa0703680 | Msa1174530 | 0.816492 | 5.377223e-52 | -8.615850e-47 |
| Msa0703680 | Msa1224840 | 0.858599 | 7.374965e-63 | -8.615850e-47 |
| Msa0703680 | Msa1235250 | 0.822845 | 1.904779e-53 | -8.615850e-47 |
| Msa0703680 | Msa1236100 | 0.811241 | 7.725178e-51 | -8.615850e-47 |
| Msa0703680 | Msa1276660 | 0.816855 | 4.459110e-52 | -8.615850e-47 |
| Msa0703680 | Msa1277490 | 0.808691 | 2.734980e-50 | -8.615850e-47 |
| Msa0703680 | Msa1338830 | 0.806978 | 6.326676e-50 | -8.615850e-47 |
| Msa0703680 | Msa1371520 | 0.831525 | 1.589284e-55 | -8.615850e-47 |
| Msa0703680 | Msa1386710 | 0.818798 | 1.624292e-52 | -8.615850e-47 |
| Msa0703680 | Msa1398860 | 0.804634 | 1.966190e-49 | -8.615850e-47 |
| Msa0703680 | Msa1439610 | 0.801432 | 9.029456e-49 | -8.615850e-47 |
| Msa0703680 | Msa1443930 | 0.814643 | 1.387497e-51 | -8.615850e-47 |
| Msa0130240 | Msa0703680 | 0.816575 | 5.152650e-52 | -8.615850e-47 |
| Msa0138360 | Msa0703680 | 0.836867 | 7.274138e-57 | -8.615850e-47 |
| Msa0144360 | Msa0703680 | 0.817348 | 3.455966e-52 | -8.615850e-47 |
| Msa0147980 | Msa0703680 | 0.838159 | 3.393904e-57 | -8.615850e-47 |
| Msa0152400 | Msa0703680 | 0.801276 | 9.720620e-49 | -8.615850e-47 |
| Msa0188890 | Msa0703680 | 0.801848 | 7.418822e-49 | -8.615850e-47 |
| Msa0364690 | Msa0703680 | 0.801541 | 8.576329e-49 | -8.615850e-47 |
| Msa0410500 | Msa0703680 | 0.801630 | 8.223402e-49 | -8.615850e-47 |
| Msa0422850 | Msa0703680 | 0.812871 | 3.409070e-51 | -8.615850e-47 |
| Msa0422930 | Msa0703680 | 0.810249 | 1.266003e-50 | -8.615850e-47 |
| Msa0479200 | Msa0703680 | 0.815717 | 8.011755e-52 | -8.615850e-47 |
| Msa0263080 | Msa0703680 | 0.803180 | 3.941961e-49 | -8.615850e-47 |
| Msa0277450 | Msa0703680 | 0.812323 | 4.490934e-51 | -8.615850e-47 |
| Msa0300270 | Msa0703680 | 0.807974 | 3.888513e-50 | -8.615850e-47 |
| Msa0300320 | Msa0703680 | 0.805698 | 1.177116e-49 | -8.615850e-47 |
| Msa0300690 | Msa0703680 | 0.811296 | 7.514956e-51 | -8.615850e-47 |
| Msa0341690 | Msa0703680 | 0.802240 | 6.163228e-49 | -8.615850e-47 |
| Msa0527190 | Msa0703680 | 0.833983 | 3.897410e-56 | -8.615850e-47 |
| Msa0556010 | Msa0703680 | 0.804012 | 2.649869e-49 | -8.615850e-47 |
| Msa0567260 | Msa0703680 | 0.946930 | 1.795917e-105 | -8.615850e-47 |
| Msa0574890 | Msa0703680 | 0.826186 | 3.114114e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703680 | MtrunA17_Chr8g0382081 | 100.000 | 309 | 0 | 0 | 1 | 309 | 1 | 309 | 0.0 | 619 |
| Msa0703680 | MtrunA17_Chr5g0436341 | 73.490 | 298 | 77 | 2 | 11 | 307 | 8 | 304 | 1.95e-160 | 449 |
| Msa0703680 | MtrunA17_Chr3g0112981 | 72.542 | 295 | 81 | 0 | 15 | 309 | 28 | 322 | 2.64e-155 | 437 |
| Msa0703680 | MtrunA17_Chr4g0008071 | 68.966 | 290 | 90 | 0 | 19 | 308 | 20 | 309 | 9.54e-146 | 412 |
| Msa0703680 | MtrunA17_Chr4g0056661 | 58.784 | 296 | 122 | 0 | 14 | 309 | 55 | 350 | 4.14e-118 | 343 |
| Msa0703680 | MtrunA17_Chr3g0103641 | 71.698 | 159 | 45 | 0 | 151 | 309 | 3 | 161 | 2.70e-77 | 233 |
| Msa0703680 | MtrunA17_Chr3g0103631 | 65.806 | 155 | 53 | 0 | 10 | 164 | 8 | 162 | 4.08e-72 | 220 |
| Msa0703680 | MtrunA17_Chr3g0114581 | 32.646 | 291 | 188 | 3 | 19 | 306 | 22 | 307 | 9.38e-49 | 166 |
| Msa0703680 | MtrunA17_Chr1g0160071 | 32.404 | 287 | 192 | 1 | 19 | 305 | 21 | 305 | 2.73e-48 | 164 |
| Msa0703680 | MtrunA17_Chr2g0307091 | 35.473 | 296 | 177 | 7 | 16 | 305 | 18 | 305 | 1.13e-34 | 129 |
| Msa0703680 | MtrunA17_Chr3g0137921 | 32.313 | 294 | 189 | 4 | 16 | 305 | 22 | 309 | 5.77e-31 | 119 |
| Msa0703680 | MtrunA17_Chr7g0270591 | 29.260 | 311 | 210 | 4 | 1 | 307 | 4 | 308 | 3.48e-27 | 109 |
| Msa0703680 | MtrunA17_Chr4g0063601 | 31.081 | 296 | 162 | 9 | 16 | 305 | 18 | 277 | 1.17e-25 | 103 |
| Msa0703680 | MtrunA17_Chr2g0312911 | 25.796 | 314 | 218 | 6 | 1 | 307 | 1 | 306 | 9.22e-23 | 96.7 |
| Msa0703680 | MtrunA17_Chr4g0069261 | 28.322 | 286 | 197 | 3 | 25 | 307 | 24 | 304 | 2.12e-22 | 96.3 |
| Msa0703680 | MtrunA17_Chr6g0449891 | 27.852 | 298 | 206 | 4 | 14 | 306 | 115 | 408 | 1.96e-18 | 85.1 |
| Msa0703680 | MtrunA17_Chr4g0058971 | 26.578 | 301 | 201 | 9 | 15 | 306 | 88 | 377 | 8.24e-18 | 83.2 |
| Msa0703680 | MtrunA17_Chr8g0341861 | 24.172 | 302 | 203 | 7 | 14 | 298 | 171 | 463 | 3.10e-15 | 75.9 |
| Msa0703680 | MtrunA17_Chr2g0298931 | 29.966 | 297 | 177 | 11 | 14 | 301 | 63 | 337 | 4.07e-15 | 75.1 |
| Msa0703680 | MtrunA17_Chr3g0117501 | 26.389 | 216 | 151 | 2 | 96 | 306 | 190 | 402 | 5.64e-13 | 68.9 |
| Msa0703680 | MtrunA17_Chr5g0419001 | 25.658 | 304 | 201 | 11 | 18 | 307 | 110 | 402 | 9.43e-13 | 68.2 |
| Msa0703680 | MtrunA17_Chr3g0129401 | 23.693 | 287 | 211 | 4 | 22 | 307 | 24 | 303 | 3.68e-11 | 63.2 |
| Msa0703680 | MtrunA17_Chr4g0046561 | 25.490 | 306 | 201 | 12 | 19 | 309 | 106 | 399 | 5.02e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703680 | AT5G04160.1 | 78.964 | 309 | 65 | 0 | 1 | 309 | 1 | 309 | 0.0 | 505 |
| Msa0703680 | AT3G10290.1 | 78.947 | 304 | 64 | 0 | 6 | 309 | 52 | 355 | 0.0 | 504 |
| Msa0703680 | AT3G11320.1 | 74.744 | 293 | 74 | 0 | 15 | 307 | 14 | 306 | 2.66e-158 | 444 |
| Msa0703680 | AT5G05820.1 | 73.038 | 293 | 79 | 0 | 15 | 307 | 14 | 306 | 2.14e-154 | 434 |
| Msa0703680 | AT1G12500.1 | 56.026 | 307 | 135 | 0 | 3 | 309 | 50 | 356 | 4.89e-120 | 349 |
| Msa0703680 | AT1G21870.1 | 33.564 | 289 | 188 | 3 | 19 | 306 | 22 | 307 | 6.13e-48 | 164 |
| Msa0703680 | AT1G77610.1 | 33.219 | 292 | 185 | 5 | 19 | 306 | 16 | 301 | 3.84e-47 | 162 |
| Msa0703680 | AT5G11230.1 | 34.459 | 296 | 180 | 7 | 16 | 305 | 22 | 309 | 9.24e-35 | 129 |
| Msa0703680 | AT5G25400.1 | 34.122 | 296 | 181 | 7 | 16 | 305 | 22 | 309 | 4.39e-33 | 125 |
| Msa0703680 | AT4G32390.1 | 34.797 | 296 | 179 | 7 | 16 | 305 | 22 | 309 | 2.03e-32 | 123 |
| Msa0703680 | AT2G25520.1 | 35.473 | 296 | 177 | 7 | 16 | 305 | 22 | 309 | 1.90e-31 | 120 |
| Msa0703680 | AT1G53660.1 | 29.066 | 289 | 190 | 6 | 25 | 307 | 30 | 309 | 1.87e-26 | 107 |
| Msa0703680 | AT1G53660.3 | 29.066 | 289 | 190 | 6 | 25 | 307 | 48 | 327 | 3.01e-26 | 106 |
| Msa0703680 | AT3G17430.2 | 29.268 | 287 | 193 | 5 | 25 | 307 | 25 | 305 | 6.00e-25 | 103 |
| Msa0703680 | AT3G17430.1 | 29.268 | 287 | 193 | 5 | 25 | 307 | 25 | 305 | 6.00e-25 | 103 |
| Msa0703680 | AT1G53660.4 | 28.832 | 274 | 182 | 5 | 38 | 307 | 11 | 275 | 6.11e-25 | 102 |
| Msa0703680 | AT3G14410.1 | 28.223 | 287 | 198 | 4 | 25 | 307 | 28 | 310 | 1.92e-23 | 99.0 |
| Msa0703680 | AT1G48230.1 | 29.825 | 285 | 190 | 5 | 25 | 305 | 25 | 303 | 1.71e-22 | 96.7 |
| Msa0703680 | AT1G53660.5 | 31.481 | 216 | 136 | 4 | 95 | 307 | 9 | 215 | 9.76e-22 | 92.0 |
| Msa0703680 | AT5G57100.1 | 30.450 | 289 | 172 | 9 | 24 | 301 | 76 | 346 | 1.91e-19 | 88.2 |
| Msa0703680 | AT5G57100.2 | 31.633 | 196 | 121 | 6 | 24 | 214 | 76 | 263 | 3.18e-16 | 77.8 |
| Msa0703680 | AT3G01550.1 | 24.407 | 295 | 213 | 5 | 17 | 306 | 84 | 373 | 1.28e-15 | 77.0 |
| Msa0703680 | AT1G48230.2 | 29.075 | 227 | 153 | 4 | 81 | 305 | 10 | 230 | 5.42e-15 | 74.3 |
| Msa0703680 | AT5G55950.4 | 30.584 | 291 | 169 | 10 | 24 | 301 | 9 | 279 | 1.17e-13 | 70.9 |
| Msa0703680 | AT1G06470.4 | 23.077 | 299 | 210 | 5 | 14 | 298 | 77 | 369 | 1.27e-13 | 71.2 |
| Msa0703680 | AT1G06470.2 | 23.077 | 299 | 210 | 5 | 14 | 298 | 77 | 369 | 1.27e-13 | 71.2 |
| Msa0703680 | AT1G06470.1 | 23.077 | 299 | 210 | 5 | 14 | 298 | 77 | 369 | 1.27e-13 | 71.2 |
| Msa0703680 | AT1G06470.3 | 23.077 | 299 | 210 | 5 | 14 | 298 | 77 | 369 | 1.27e-13 | 71.2 |
| Msa0703680 | AT5G55950.1 | 30.584 | 291 | 169 | 10 | 24 | 301 | 69 | 339 | 1.72e-13 | 70.9 |
| Msa0703680 | AT5G55950.2 | 32.828 | 198 | 116 | 7 | 24 | 214 | 69 | 256 | 1.42e-12 | 67.4 |
| Msa0703680 | AT3G01550.2 | 23.729 | 295 | 210 | 6 | 17 | 306 | 84 | 368 | 2.07e-12 | 67.4 |
| Msa0703680 | AT5G57100.3 | 36.879 | 141 | 78 | 5 | 24 | 159 | 76 | 210 | 2.98e-12 | 66.2 |
| Msa0703680 | AT5G46110.2 | 26.821 | 302 | 197 | 13 | 18 | 309 | 1 | 288 | 3.07e-12 | 66.6 |
| Msa0703680 | AT5G46110.4 | 26.821 | 302 | 197 | 13 | 18 | 309 | 114 | 401 | 1.25e-11 | 65.5 |
| Msa0703680 | AT5G46110.1 | 27.036 | 307 | 193 | 14 | 18 | 307 | 114 | 406 | 1.67e-11 | 65.1 |
| Msa0703680 | AT4G39390.1 | 25.850 | 294 | 196 | 7 | 22 | 307 | 29 | 308 | 1.73e-11 | 64.7 |
| Msa0703680 | AT4G39390.2 | 25.850 | 294 | 196 | 7 | 22 | 307 | 29 | 308 | 1.73e-11 | 64.7 |
| Msa0703680 | AT4G39390.3 | 26.190 | 294 | 195 | 8 | 22 | 307 | 25 | 304 | 1.88e-11 | 64.3 |
Find 47 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAAAGAACATAAATTTA+TGG | 0.161882 | 4_4:-75676158 | Msa0703680:CDS |
| AAGTACCTTATCTCAAATTA+TGG | 0.221981 | 4_4:-75677982 | Msa0703680:CDS |
| ATCAAGGTCACAGTTCTTAA+AGG | 0.247185 | 4_4:-75677854 | Msa0703680:CDS |
| TGGTGATGGCAGGGTGAGCC+TGG | 0.262017 | 4_4:-75677107 | Msa0703680:intron |
| CAATATCTCTCTCAAGTATT+TGG | 0.275503 | 4_4:-75677788 | Msa0703680:CDS |
| AGCTTTCAAATCTGTTCTTC+AGG | 0.288805 | 4_4:-75677036 | Msa0703680:CDS |
| GAGCCTGGGTTTCACTTGTT+TGG | 0.295030 | 4_4:-75677092 | Msa0703680:CDS |
| AATTATGTTAATCAGGTATT+AGG | 0.309168 | 4_4:-75675739 | Msa0703680:intron |
| CAATAAAAGTGACAGGGTTT+TGG | 0.309718 | 4_4:+75675671 | None:intergenic |
| GCTCTTGTTCCTGTTGTTGC+TGG | 0.312689 | 4_4:-75677664 | Msa0703680:CDS |
| CTTAACTCAGCCGCAGCATA+TGG | 0.344628 | 4_4:-75676121 | Msa0703680:CDS |
| GGAAAAGAACATAAATTTAT+GGG | 0.351125 | 4_4:-75676157 | Msa0703680:CDS |
| AGCAAACTTAACAAACTTCT+TGG | 0.373328 | 4_4:-75676098 | Msa0703680:CDS |
| GGCTACTCAGTTACTGTAAT+GGG | 0.394558 | 4_4:-75675640 | Msa0703680:CDS |
| AACATGAATTCTTTGTTGTT+TGG | 0.399155 | 4_4:+75678063 | None:intergenic |
| GCTTTCAAATCTGTTCTTCA+GGG | 0.437125 | 4_4:-75677035 | Msa0703680:CDS |
| TTGCATACGGAGAAACAAAA+AGG | 0.447859 | 4_4:-75675612 | Msa0703680:CDS |
| TGACACATTGTGAGGAAGAT+TGG | 0.449241 | 4_4:+75677949 | None:intergenic |
| TGAAACCATAATTTGAGATA+AGG | 0.466205 | 4_4:+75677977 | None:intergenic |
| GGTGATGGCAGGGTGAGCCT+GGG | 0.473562 | 4_4:-75677106 | Msa0703680:intron |
| GCCATGCCAATAAAAGTGAC+AGG | 0.492901 | 4_4:+75675664 | None:intergenic |
| ATGGCAACACCAGCAACAAC+AGG | 0.493877 | 4_4:+75677655 | None:intergenic |
| CTCTTGCAGCGGTTGCACTT+AGG | 0.496429 | 4_4:+75677057 | None:intergenic |
| AGCGTGATATCTAAAACATT+CGG | 0.497597 | 4_4:+75676187 | None:intergenic |
| TGGCTACTCAGTTACTGTAA+TGG | 0.499480 | 4_4:-75675641 | Msa0703680:CDS |
| CTGCGATTGGAGACATATAC+AGG | 0.502629 | 4_4:+75676245 | None:intergenic |
| TACAAGTGCTTTGACACTCC+AGG | 0.510037 | 4_4:-75676065 | Msa0703680:intron |
| TTAGATATCACGCTATCACT+TGG | 0.514237 | 4_4:-75676178 | Msa0703680:CDS |
| GTTTCTTTCAACCAAGCTGT+AGG | 0.516484 | 4_4:-75677763 | Msa0703680:CDS |
| CTCAACAGATGATTAAATCA+AGG | 0.523888 | 4_4:-75677870 | Msa0703680:CDS |
| GGCTCCATGAAAACTACTGC+AGG | 0.527890 | 4_4:+75676208 | None:intergenic |
| CAGGTATTAGGCAATGCAAA+AGG | 0.533740 | 4_4:-75675727 | Msa0703680:CDS |
| GCTGGTGTTGCCATTGCAAG+TGG | 0.549715 | 4_4:-75677646 | Msa0703680:CDS |
| CTTGATTTAATCATCTGTTG+AGG | 0.555444 | 4_4:+75677871 | None:intergenic |
| AAAACATACCCCACTTGCAA+TGG | 0.557860 | 4_4:+75677636 | None:intergenic |
| CTGGTGTTGCCATTGCAAGT+GGG | 0.559180 | 4_4:-75677645 | Msa0703680:CDS |
| CTTTGGTACTCATCAAACAT+TGG | 0.563810 | 4_4:-75678021 | Msa0703680:CDS |
| TGGTGTTGCCATTGCAAGTG+GGG | 0.597605 | 4_4:-75677644 | Msa0703680:intron |
| GTAATGGGAGTTATTGCATA+CGG | 0.599804 | 4_4:-75675625 | Msa0703680:CDS |
| AATCCAAACAAGTGAAACCC+AGG | 0.628421 | 4_4:+75677089 | None:intergenic |
| CCATGCCAATAAAAGTGACA+GGG | 0.651043 | 4_4:+75675665 | None:intergenic |
| GTGTGGTTGCACCTACAGCT+TGG | 0.651188 | 4_4:+75677752 | None:intergenic |
| AACAATCTTAAAGAACACAA+TGG | 0.654847 | 4_4:+75677894 | None:intergenic |
| TAAGTTTGCTCCATATGCTG+CGG | 0.688898 | 4_4:+75676111 | None:intergenic |
| AGATTTGAAAGCTCTTGCAG+CGG | 0.693952 | 4_4:+75677046 | None:intergenic |
| AACAGCAGTGAAGAAAGGTG+TGG | 0.696609 | 4_4:+75677735 | None:intergenic |
| CTGTCATGTGACACATTGTG+AGG | 0.735310 | 4_4:+75677941 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATAATTGCTTATAATAT+AGG | - | chr4_4:75676103-75676122 | Msa0703680:CDS | 10.0% |
| !! | AACAAATTAAAATTATCATA+TGG | + | chr4_4:75676507-75676526 | None:intergenic | 10.0% |
| !!! | TGATAATTTTAATTTGTTAT+TGG | - | chr4_4:75676509-75676528 | Msa0703680:intron | 10.0% |
| !! | TTTATAATCAATTAGCATTT+AGG | - | chr4_4:75676371-75676390 | Msa0703680:intron | 15.0% |
| !! | TTAAGAATCCAAAAAAATAT+GGG | + | chr4_4:75677301-75677320 | None:intergenic | 15.0% |
| !! | ATTAAGAATCCAAAAAAATA+TGG | + | chr4_4:75677302-75677321 | None:intergenic | 15.0% |
| !!! | TAATTATATTTTTAGCTTTG+AGG | + | chr4_4:75677687-75677706 | None:intergenic | 15.0% |
| !!! | GTTTTTTTTGTTTGATTTGA+TGG | - | chr4_4:75676054-75676073 | Msa0703680:intron | 20.0% |
| !!! | GAAATTATACATAGATGTTT+TGG | - | chr4_4:75676157-75676176 | Msa0703680:CDS | 20.0% |
| !!! | TGGTTTTGATTTTGAAATTA+TGG | - | chr4_4:75676177-75676196 | Msa0703680:CDS | 20.0% |
| !!! | TTTTGAAATTATGGATGTTT+TGG | - | chr4_4:75676186-75676205 | Msa0703680:CDS | 20.0% |
| !!! | GATTTTGGATTTTAATTTTC+TGG | - | chr4_4:75676285-75676304 | Msa0703680:intron | 20.0% |
| !! | TATAAAAATACCAAACAATC+TGG | + | chr4_4:75676357-75676376 | None:intergenic | 20.0% |
| !!! | CTAATTCATGACAATTTTTA+TGG | + | chr4_4:75676450-75676469 | None:intergenic | 20.0% |
| !! | CATAAAAATTGTCATGAATT+AGG | - | chr4_4:75676448-75676467 | Msa0703680:intron | 20.0% |
| !! | ATTAAAATTATCATATGGTG+TGG | + | chr4_4:75676502-75676521 | None:intergenic | 20.0% |
| !!! | ACATTTTTTTAATACGTTGT+TGG | - | chr4_4:75676545-75676564 | Msa0703680:intron | 20.0% |
| !!! | ATTTTATAGTACTGTTCTAA+AGG | - | chr4_4:75676781-75676800 | Msa0703680:intron | 20.0% |
| !! | CATATAAAAAATGAGATTCA+AGG | + | chr4_4:75676898-75676917 | None:intergenic | 20.0% |
| !! | TAAGAATCCAAAAAAATATG+GGG | + | chr4_4:75677300-75677319 | None:intergenic | 20.0% |
| !! | TGGAAAAGAACATAAATTTA+TGG | - | chr4_4:75677514-75677533 | Msa0703680:intron | 20.0% |
| !! | GGAAAAGAACATAAATTTAT+GGG | - | chr4_4:75677515-75677534 | Msa0703680:intron | 20.0% |
| !! | ATTATTATTACAACCTTCAA+TGG | - | chr4_4:75677726-75677745 | Msa0703680:CDS | 20.0% |
| !! | AATTATGTTAATCAGGTATT+AGG | - | chr4_4:75677933-75677952 | Msa0703680:CDS | 20.0% |
| !!! | AACATGAATTCTTTGTTGTT+TGG | + | chr4_4:75675612-75675631 | None:intergenic | 25.0% |
| ! | AAGTACCTTATCTCAAATTA+TGG | - | chr4_4:75675690-75675709 | Msa0703680:CDS | 25.0% |
| !! | TGAAACCATAATTTGAGATA+AGG | + | chr4_4:75675698-75675717 | None:intergenic | 25.0% |
| ! | AACAATCTTAAAGAACACAA+TGG | + | chr4_4:75675781-75675800 | None:intergenic | 25.0% |
| !! | TATAATATAGGTGTGTTGAT+TGG | - | chr4_4:75676115-75676134 | Msa0703680:CDS | 25.0% |
| ! | ATTAACTATATTTCCAGATC+TGG | + | chr4_4:75676233-75676252 | None:intergenic | 25.0% |
| !!! | TTTTTCAAATCTGTGACATT+TGG | - | chr4_4:75676263-75676282 | Msa0703680:CDS | 25.0% |
| !!! | TGTTATTTCATCTATTCATG+TGG | - | chr4_4:75676407-75676426 | Msa0703680:intron | 25.0% |
| !! | TTCATGACAATTTTTATGGT+AGG | + | chr4_4:75676446-75676465 | None:intergenic | 25.0% |
| ! | TAAAAAAATGTACACCATGT+TGG | + | chr4_4:75676536-75676555 | None:intergenic | 25.0% |
| ! | TATTTAGTTATTGTGAACTG+TGG | - | chr4_4:75676732-75676751 | Msa0703680:intron | 25.0% |
| ! | TTTATTATTCAGCTCTTTGT+TGG | - | chr4_4:75676809-75676828 | Msa0703680:intron | 25.0% |
| ! | TTCAATTGTGTATGAACAAT+GGG | + | chr4_4:75677052-75677071 | None:intergenic | 25.0% |
| ! | TTTCAATTGTGTATGAACAA+TGG | + | chr4_4:75677053-75677072 | None:intergenic | 25.0% |
| !! | CATCAGTGTATTAAAACTTT+TGG | + | chr4_4:75677079-75677098 | None:intergenic | 25.0% |
| ! | TATTTATCGCAAAGTAAAGA+AGG | + | chr4_4:75677216-75677235 | None:intergenic | 25.0% |
| ! | CTTTACTTTGCGATAAATAT+TGG | - | chr4_4:75677217-75677236 | Msa0703680:intron | 25.0% |
| !! | ATGTACATAAAAGTCACTTT+CGG | + | chr4_4:75677756-75677775 | None:intergenic | 25.0% |
| ! | TCTGTCAAATTATGTTAATC+AGG | - | chr4_4:75677926-75677945 | Msa0703680:CDS | 25.0% |
| ! | CATGTTCATATGTTTTCTTG+TGG | - | chr4_4:75675626-75675645 | Msa0703680:CDS | 30.0% |
| CTTGATTTAATCATCTGTTG+AGG | + | chr4_4:75675804-75675823 | None:intergenic | 30.0% | |
| CTCAACAGATGATTAAATCA+AGG | - | chr4_4:75675802-75675821 | Msa0703680:intron | 30.0% | |
| !!! | ACTTTGAGCTTTGTTTTTTG+TGG | - | chr4_4:75675846-75675865 | Msa0703680:intron | 30.0% |
| !!! | CTTTGAGCTTTGTTTTTTGT+GGG | - | chr4_4:75675847-75675866 | Msa0703680:intron | 30.0% |
| CAATATCTCTCTCAAGTATT+TGG | - | chr4_4:75675884-75675903 | Msa0703680:intron | 30.0% | |
| !!! | TTTAGCTACTTTTAAGAGAG+AGG | - | chr4_4:75675968-75675987 | Msa0703680:intron | 30.0% |
| ! | AATCTGTGACATTTGGATTT+TGG | - | chr4_4:75676270-75676289 | Msa0703680:CDS | 30.0% |
| !! | AATTTGTTATTGGACCAACA+TGG | - | chr4_4:75676519-75676538 | Msa0703680:intron | 30.0% |
| !! | TTTTAATACGTTGTTGGTGA+TGG | - | chr4_4:75676551-75676570 | Msa0703680:intron | 30.0% |
| ! | GCATTTTACTTTCTTCTGAA+GGG | - | chr4_4:75676659-75676678 | Msa0703680:intron | 30.0% |
| ATGCTTAATAAGCTGTGAAA+GGG | + | chr4_4:75677000-75677019 | None:intergenic | 30.0% | |
| TATGCTTAATAAGCTGTGAA+AGG | + | chr4_4:75677001-75677020 | None:intergenic | 30.0% | |
| TATGGTCAATTTCTATAGCT+TGG | + | chr4_4:75677260-75677279 | None:intergenic | 30.0% | |
| TAGAAATTGACCATAACTTG+AGG | - | chr4_4:75677265-75677284 | Msa0703680:intron | 30.0% | |
| ! | AGCGTGATATCTAAAACATT+CGG | + | chr4_4:75677488-75677507 | None:intergenic | 30.0% |
| AGCAAACTTAACAAACTTCT+TGG | - | chr4_4:75677574-75677593 | Msa0703680:intron | 30.0% | |
| GAGATAAAAAAAGACAAACC+TGG | + | chr4_4:75677628-75677647 | None:intergenic | 30.0% | |
| !! | GTGCTCATGATTTTTATTTG+TGG | + | chr4_4:75677657-75677676 | None:intergenic | 30.0% |
| ! | TATGTTTTCTTGTGGCTCTT+TGG | - | chr4_4:75675634-75675653 | Msa0703680:CDS | 35.0% |
| CTTTGGTACTCATCAAACAT+TGG | - | chr4_4:75675651-75675670 | Msa0703680:CDS | 35.0% | |
| ATCAAGGTCACAGTTCTTAA+AGG | - | chr4_4:75675818-75675837 | Msa0703680:intron | 35.0% | |
| ! | TATATTTCCAGATCTGGAGT+GGG | + | chr4_4:75676227-75676246 | None:intergenic | 35.0% |
| AGCTTTCAAATCTGTTCTTC+AGG | - | chr4_4:75676636-75676655 | Msa0703680:intron | 35.0% | |
| GCTTTCAAATCTGTTCTTCA+GGG | - | chr4_4:75676637-75676656 | Msa0703680:intron | 35.0% | |
| ! | GGCATTTTACTTTCTTCTGA+AGG | - | chr4_4:75676658-75676677 | Msa0703680:intron | 35.0% |
| TTAGCCACCAAACATCATTA+AGG | - | chr4_4:75676968-75676987 | Msa0703680:intron | 35.0% | |
| TTCTCCTTAATGATGTTTGG+TGG | + | chr4_4:75676975-75676994 | None:intergenic | 35.0% | |
| GTGTTCTCCTTAATGATGTT+TGG | + | chr4_4:75676978-75676997 | None:intergenic | 35.0% | |
| AAGCAAAAGCAATGTTAAGC+TGG | + | chr4_4:75677185-75677204 | None:intergenic | 35.0% | |
| GAATCCAAAAAAATATGGGG+AGG | + | chr4_4:75677297-75677316 | None:intergenic | 35.0% | |
| TGCATAATCCTCGTTACATA+TGG | - | chr4_4:75677332-75677351 | Msa0703680:intron | 35.0% | |
| AACAAAAATACGACTGCGAT+TGG | + | chr4_4:75677443-75677462 | None:intergenic | 35.0% | |
| TTAGATATCACGCTATCACT+TGG | - | chr4_4:75677494-75677513 | Msa0703680:intron | 35.0% | |
| AACAAAGCTGAGTTAGCAAT+AGG | + | chr4_4:75677879-75677898 | None:intergenic | 35.0% | |
| ! | CAATAAAAGTGACAGGGTTT+TGG | + | chr4_4:75678004-75678023 | None:intergenic | 35.0% |
| !!! | CAAAACCCTGTCACTTTTAT+TGG | - | chr4_4:75678002-75678021 | Msa0703680:CDS | 35.0% |
| ! | GTAATGGGAGTTATTGCATA+CGG | - | chr4_4:75678047-75678066 | Msa0703680:CDS | 35.0% |
| TTGCATACGGAGAAACAAAA+AGG | - | chr4_4:75678060-75678079 | Msa0703680:CDS | 35.0% | |
| TGACACATTGTGAGGAAGAT+TGG | + | chr4_4:75675726-75675745 | None:intergenic | 40.0% | |
| !!! | CTTTGTTTTTTGTGGGTCTG+TGG | - | chr4_4:75675854-75675873 | Msa0703680:intron | 40.0% |
| GTTTCTTTCAACCAAGCTGT+AGG | - | chr4_4:75675909-75675928 | Msa0703680:intron | 40.0% | |
| GCAAAAACAGCAGTGAAGAA+AGG | + | chr4_4:75675945-75675964 | None:intergenic | 40.0% | |
| !! | CTACTTTTAAGAGAGAGGCT+TGG | - | chr4_4:75675973-75675992 | Msa0703680:intron | 40.0% |
| AAAACATACCCCACTTGCAA+TGG | + | chr4_4:75676039-75676058 | None:intergenic | 40.0% | |
| CTATATTTCCAGATCTGGAG+TGG | + | chr4_4:75676228-75676247 | None:intergenic | 40.0% | |
| TAGCAGATAGCCAGATTGTT+TGG | - | chr4_4:75676344-75676363 | Msa0703680:intron | 40.0% | |
| AATCCAAACAAGTGAAACCC+AGG | + | chr4_4:75676586-75676605 | None:intergenic | 40.0% | |
| AGATTTGAAAGCTCTTGCAG+CGG | + | chr4_4:75676629-75676648 | None:intergenic | 40.0% | |
| CCAAAAAAATATGGGGAGGA+AGG | + | chr4_4:75677293-75677312 | None:intergenic | 40.0% | |
| !!! | CCTTCCTCCCCATATTTTTT+TGG | - | chr4_4:75677290-75677309 | Msa0703680:intron | 40.0% |
| GTTCTTTGCCATATGTAACG+AGG | + | chr4_4:75677343-75677362 | None:intergenic | 40.0% | |
| TTCATCCATTTCAACCTACC+AGG | - | chr4_4:75677378-75677397 | Msa0703680:intron | 40.0% | |
| TCATCCATTTCAACCTACCA+GGG | - | chr4_4:75677379-75677398 | Msa0703680:intron | 40.0% | |
| ! | TAAGTTTGCTCCATATGCTG+CGG | + | chr4_4:75677564-75677583 | None:intergenic | 40.0% |
| CAGGTATTAGGCAATGCAAA+AGG | - | chr4_4:75677945-75677964 | Msa0703680:CDS | 40.0% | |
| CCATGCCAATAAAAGTGACA+GGG | + | chr4_4:75678010-75678029 | None:intergenic | 40.0% | |
| TGGCTACTCAGTTACTGTAA+TGG | - | chr4_4:75678031-75678050 | Msa0703680:CDS | 40.0% | |
| GGCTACTCAGTTACTGTAAT+GGG | - | chr4_4:75678032-75678051 | Msa0703680:CDS | 40.0% | |
| CATACGGAGAAACAAAAAGG+AGG | - | chr4_4:75678063-75678082 | Msa0703680:CDS | 40.0% | |
| CTGTCATGTGACACATTGTG+AGG | + | chr4_4:75675734-75675753 | None:intergenic | 45.0% | |
| !!! | GTTTTTTGTGGGTCTGTGGT+TGG | - | chr4_4:75675858-75675877 | Msa0703680:intron | 45.0% |
| AACAGCAGTGAAGAAAGGTG+TGG | + | chr4_4:75675940-75675959 | None:intergenic | 45.0% | |
| TTTCTGGATCTGCACTCATC+TGG | - | chr4_4:75676301-75676320 | Msa0703680:intron | 45.0% | |
| !! | AATACGTTGTTGGTGATGGC+AGG | - | chr4_4:75676555-75676574 | Msa0703680:intron | 45.0% |
| !! | ATACGTTGTTGGTGATGGCA+GGG | - | chr4_4:75676556-75676575 | Msa0703680:intron | 45.0% |
| ! | TTCTCCCTGGTAGGTTGAAA+TGG | + | chr4_4:75677386-75677405 | None:intergenic | 45.0% |
| CATTGAGTTCAACTTCTCCC+TGG | + | chr4_4:75677399-75677418 | None:intergenic | 45.0% | |
| CTGCGATTGGAGACATATAC+AGG | + | chr4_4:75677430-75677449 | None:intergenic | 45.0% | |
| ! | GTTGCCTGCAGTAGTTTTCA+TGG | - | chr4_4:75677460-75677479 | Msa0703680:intron | 45.0% |
| TACAAGTGCTTTGACACTCC+AGG | - | chr4_4:75677607-75677626 | Msa0703680:intron | 45.0% | |
| ! | CACTTTCGGTTTGCCATTGA+AGG | + | chr4_4:75677742-75677761 | None:intergenic | 45.0% |
| !!! | AAGTGACAGGGTTTTGGAAC+AGG | + | chr4_4:75677998-75678017 | None:intergenic | 45.0% |
| !!! | CCCTGTCACTTTTATTGGCA+TGG | - | chr4_4:75678007-75678026 | Msa0703680:CDS | 45.0% |
| GCCATGCCAATAAAAGTGAC+AGG | + | chr4_4:75678011-75678030 | None:intergenic | 45.0% | |
| !!! | GTCACTTTTATTGGCATGGC+TGG | - | chr4_4:75678011-75678030 | Msa0703680:CDS | 45.0% |
| ! | TTTTGTGGGTCTGTGGTTGG+TGG | - | chr4_4:75675861-75675880 | Msa0703680:intron | 50.0% |
| GCTCTTGTTCCTGTTGTTGC+TGG | - | chr4_4:75676008-75676027 | Msa0703680:intron | 50.0% | |
| ATGGCAACACCAGCAACAAC+AGG | + | chr4_4:75676020-75676039 | None:intergenic | 50.0% | |
| !! | CTGGTGTTGCCATTGCAAGT+GGG | - | chr4_4:75676027-75676046 | Msa0703680:intron | 50.0% |
| !! | TGGTGTTGCCATTGCAAGTG+GGG | - | chr4_4:75676028-75676047 | Msa0703680:intron | 50.0% |
| ! | CCAGATCTGGAGTGGGATTA+TGG | + | chr4_4:75676220-75676239 | None:intergenic | 50.0% |
| CCATAATCCCACTCCAGATC+TGG | - | chr4_4:75676217-75676236 | Msa0703680:CDS | 50.0% | |
| GAGCCTGGGTTTCACTTGTT+TGG | - | chr4_4:75676580-75676599 | Msa0703680:intron | 50.0% | |
| !! | GAGGAAGGCACCTCAAGTTA+TGG | + | chr4_4:75677278-75677297 | None:intergenic | 50.0% |
| GAGTTCAACTTCTCCCTGGT+AGG | + | chr4_4:75677395-75677414 | None:intergenic | 50.0% | |
| GGCTCCATGAAAACTACTGC+AGG | + | chr4_4:75677467-75677486 | None:intergenic | 50.0% | |
| CTTAACTCAGCCGCAGCATA+TGG | - | chr4_4:75677551-75677570 | Msa0703680:intron | 50.0% | |
| GTGTGGTTGCACCTACAGCT+TGG | + | chr4_4:75675923-75675942 | None:intergenic | 55.0% | |
| !! | GCTGGTGTTGCCATTGCAAG+TGG | - | chr4_4:75676026-75676045 | Msa0703680:intron | 55.0% |
| CTCTTGCAGCGGTTGCACTT+AGG | + | chr4_4:75676618-75676637 | None:intergenic | 55.0% | |
| !! | TGGTGATGGCAGGGTGAGCC+TGG | - | chr4_4:75676565-75676584 | Msa0703680:intron | 65.0% |
| ! | GGTGATGGCAGGGTGAGCCT+GGG | - | chr4_4:75676566-75676585 | Msa0703680:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 75675600 | 75678094 | 75675600 | ID=Msa0703680;Name=Msa0703680 |
| chr4_4 | mRNA | 75675600 | 75678094 | 75675600 | ID=Msa0703680-mRNA-1;Parent=Msa0703680;Name=Msa0703680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|4|0|309 |
| chr4_4 | exon | 75677645 | 75678094 | 75677645 | ID=Msa0703680-mRNA-1:exon:23778;Parent=Msa0703680-mRNA-1 |
| chr4_4 | exon | 75677014 | 75677117 | 75677014 | ID=Msa0703680-mRNA-1:exon:23777;Parent=Msa0703680-mRNA-1 |
| chr4_4 | exon | 75676066 | 75676294 | 75676066 | ID=Msa0703680-mRNA-1:exon:23776;Parent=Msa0703680-mRNA-1 |
| chr4_4 | exon | 75675600 | 75675746 | 75675600 | ID=Msa0703680-mRNA-1:exon:23775;Parent=Msa0703680-mRNA-1 |
| chr4_4 | CDS | 75677645 | 75678094 | 75677645 | ID=Msa0703680-mRNA-1:cds;Parent=Msa0703680-mRNA-1 |
| chr4_4 | CDS | 75677014 | 75677117 | 75677014 | ID=Msa0703680-mRNA-1:cds;Parent=Msa0703680-mRNA-1 |
| chr4_4 | CDS | 75676066 | 75676294 | 75676066 | ID=Msa0703680-mRNA-1:cds;Parent=Msa0703680-mRNA-1 |
| chr4_4 | CDS | 75675600 | 75675746 | 75675600 | ID=Msa0703680-mRNA-1:cds;Parent=Msa0703680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
