AlfalfaGEDB | Msa1300850

M. sativa cultivar zhongmu-4 / Msa1300850

KEGG: -
GO: -
PFAM: PF04690.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300850	XP_003609353.2	95.812	191	6	1	1	189	1	191	1.07e-132	379

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300850	sp\|Q8GW46\|YAB5_ARATH	75.301	166	35	2	7	172	4	163	7.05e-87	255

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300850	A0A396IM32	95.812	191	6	1	1	189	1	191	5.11e-133	379

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TFs/TRs:

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Gene ID	Type	Classification
Msa1300850	TF	C2C2-YABBY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0047630	Msa1300850	0.801274	9.726591e-49	-8.615850e-47
Msa0094060	Msa1300850	0.835317	1.800547e-56	-8.615850e-47
Msa0099830	Msa1300850	0.832632	8.463862e-56	-8.615850e-47
Msa0104860	Msa1300850	-0.801034	1.089182e-48	-8.615850e-47
Msa0184070	Msa1300850	0.825629	4.224770e-54	-8.615850e-47
Msa0204000	Msa1300850	0.803006	4.283362e-49	-8.615850e-47
Msa0224700	Msa1300850	0.842439	2.583760e-58	-8.615850e-47
Msa0247340	Msa1300850	0.817580	3.063705e-52	-8.615850e-47
Msa0265780	Msa1300850	0.836150	1.107522e-56	-8.615850e-47
Msa0303780	Msa1300850	0.848878	4.629211e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300850	MtrunA17_Chr4g0063931	95.812	191	6	1	1	189	1	191	9.83e-137	379
Msa1300850	MtrunA17_Chr2g0322221	56.667	180	67	4	5	173	2	181	3.50e-60	186
Msa1300850	MtrunA17_Chr4g0011841	53.403	191	63	4	14	184	25	209	2.08e-54	172
Msa1300850	MtrunA17_Chr4g0025361	56.647	173	61	6	5	173	2	164	4.67e-53	167
Msa1300850	MtrunA17_Chr4g0056521	54.971	171	59	4	8	163	14	181	1.82e-50	162
Msa1300850	MtrunA17_Chr5g0413381	49.686	159	58	4	12	156	12	162	1.59e-45	149
Msa1300850	MtrunA17_Chr5g0419871	41.830	153	82	2	5	155	8	155	5.38e-32	113

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300850	AT2G26580.2	75.301	166	35	2	7	172	4	163	7.16e-88	255
Msa1300850	AT2G26580.1	75.301	166	35	2	7	172	4	163	7.16e-88	255
Msa1300850	AT1G08465.1	53.631	179	60	5	5	171	2	169	5.45e-56	175
Msa1300850	AT2G45190.1	54.802	177	63	6	4	163	14	190	2.31e-54	172
Msa1300850	AT4G00180.1	50.811	185	57	4	12	165	23	204	1.47e-47	155
Msa1300850	AT4G00180.2	48.864	176	56	4	21	165	1	173	2.84e-39	133
Msa1300850	AT1G23420.1	47.205	161	71	3	13	159	20	180	1.05e-38	132
Msa1300850	AT1G23420.2	47.205	161	71	3	13	159	51	211	3.06e-38	132
Msa1300850	AT2G45190.2	92.982	57	4	0	107	163	70	126	4.44e-34	119
Msa1300850	AT1G69180.1	42.069	145	78	2	11	155	18	156	4.05e-32	114

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Find 50 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTATGGTCTGTAAACA+TGG	0.552321	8_3:-62537698	Msa1300850:CDS
AACCCTTTCCTCAGTAACAT+GGG	0.626838	8_3:+62536357	None:intergenic
AATTTCCAGCTCCCGAGAAG+AGG	0.540719	8_3:-62536090	Msa1300850:intron
ACAATATTGCAAAAGTTGCA+AGG	0.540036	8_3:+62538785	None:intergenic
ACCAACCACCCATGTTACTG+AGG	0.669098	8_3:-62536365	Msa1300850:CDS
ACCGCTTACTACTTGTTTAA+TGG	0.111264	8_3:-62535393	Msa1300850:three_prime_UTR
ACTGCATGAATCCAGAGTAT+AGG	0.501971	8_3:-62536441	Msa1300850:CDS
ACTGTCATGGCAAGATGTTC+AGG	0.423195	8_3:-62537659	Msa1300850:intron
AGAAACTGATAAGGATGACA+AGG	0.582761	8_3:+62535334	None:intergenic
AGACATGTCTAGCTGTAGCA+TGG	0.594915	8_3:-62538840	Msa1300850:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATATTGCAAAAGTTGCAA+GGG	+	chr8_3:62535424-62535443	None:intergenic	30.0%
ACAATATTGCAAAAGTTGCA+AGG	+	chr8_3:62535425-62535444	None:intergenic	30.0%
TCAGATCCAAGTAAACTATA+AGG	+	chr8_3:62535482-62535501	None:intergenic	30.0%
ATATATATGTAGCATGCAAG+AGG	+	chr8_3:62535512-62535531	None:intergenic	30.0%
GTGTACATATCCAAGTATAA+AGG	-	chr8_3:62535648-62535667	Msa1300850:intron	30.0%
AATTCATTCAGATCCTGAAA+AGG	-	chr8_3:62535671-62535690	Msa1300850:intron	30.0%
GAAAGAAAGAGAAAGAGAAA+GGG	+	chr8_3:62535897-62535916	None:intergenic	30.0%
TGAAAGAAAGAGAAAGAGAA+AGG	+	chr8_3:62535898-62535917	None:intergenic	30.0%
CTTTCTTTCAAGCTAAAACA+AGG	-	chr8_3:62535908-62535927	Msa1300850:CDS	30.0%
AAAACAAGGCATATAGAGAA+AGG	-	chr8_3:62535922-62535941	Msa1300850:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	62535269	62538960	62535269	ID=Msa1300850;Name=Msa1300850
chr8_3	mRNA	62535269	62538960	62535269	ID=Msa1300850-mRNA-1;Parent=Msa1300850;Name=Msa1300850-mRNA-1;_AED=0.06;_eAED=0.06;_QI=102\|1\|1\|1\|1\|1\|7\|299\|189
chr8_3	exon	62535269	62535615	62535269	ID=Msa1300850-mRNA-1:exon:18595;Parent=Msa1300850-mRNA-1
chr8_3	exon	62535714	62535782	62535714	ID=Msa1300850-mRNA-1:exon:18594;Parent=Msa1300850-mRNA-1
chr8_3	exon	62535867	62535942	62535867	ID=Msa1300850-mRNA-1:exon:18593;Parent=Msa1300850-mRNA-1
chr8_3	exon	62536055	62536103	62536055	ID=Msa1300850-mRNA-1:exon:18592;Parent=Msa1300850-mRNA-1
chr8_3	exon	62536347	62536473	62536347	ID=Msa1300850-mRNA-1:exon:18591;Parent=Msa1300850-mRNA-1
chr8_3	exon	62537660	62537779	62537660	ID=Msa1300850-mRNA-1:exon:18590;Parent=Msa1300850-mRNA-1
chr8_3	exon	62538778	62538960	62538778	ID=Msa1300850-mRNA-1:exon:18589;Parent=Msa1300850-mRNA-1
chr8_3	five_prime_UTR	62538859	62538960	62538859	ID=Msa1300850-mRNA-1:five_prime_utr;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62538778	62538858	62538778	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62537660	62537779	62537660	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62536347	62536473	62536347	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62536055	62536103	62536055	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62535867	62535942	62535867	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62535714	62535782	62535714	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	CDS	62535568	62535615	62535568	ID=Msa1300850-mRNA-1:cds;Parent=Msa1300850-mRNA-1
chr8_3	three_prime_UTR	62535269	62535567	62535269	ID=Msa1300850-mRNA-1:three_prime_utr;Parent=Msa1300850-mRNA-1

Gene Sequence

>Msa1300850

ATGTCTAGCTGTAGCATGGATGTTGCACCTGCTGAACAACTCTGCTACATCCCTTGCAACTTTTGCAATATTGTTCTTGCGGTGAGTGTTCCATGCAGTAGCTTGTTTGAAATAGTGACCGTTCGATGTGGTCACTGCACAAATCTATGGTCTGTAAACATGGCCGCCGCGTTTCAGTCACTGTCATGGCAAGATGTTCAGGCACCTAGCCACTGCATGAATCCAGAGTATAGGATTCGAACTAGCTCCACTCCCAAATGCAACGATAGGATTGCAATGCGTTCTGCACCAACCACCCATGTTACTGAGGAAAGGGTTGTTAACCGCCCTCCCGAGAAGAGGCAACGTGTACCTTCTGCTTATAATCAGTTCATCAAGGAAGAGATTCAGAGGATCAAGGCTAATAATCCTGATATCAGTCACAGAGAAGCTTTTAGTACGGCTGCAAAGAACTGGGCACATTTTCCTCATATTCATTTTGGGCTGATGTTGGAGAGCAACAATCAAGCTAAGATGGAGACTGTCTCTGAGAAGCGTTTGATGTCTACGGCTGCGCTAATGAACAAATGA

Protein sequence

>Msa1300850

MSSCSMDVAPAEQLCYIPCNFCNIVLAVSVPCSSLFEIVTVRCGHCTNLWSVNMAAAFQSLSWQDVQAPSHCMNPEYRIRTSSTPKCNDRIAMRSAPTTHVTEERVVNRPPEKRQRVPSAYNQFIKEEIQRIKANNPDISHREAFSTAAKNWAHFPHIHFGLMLESNNQAKMETVSEKRLMSTAALMNK*