AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184070 MtrunA17_Chr7g0274951 97.955 440 9 0 1 440 1 440 0.0 902
Msa0184070 MtrunA17_Chr1g0189961 71.556 450 111 6 5 439 3 450 0.0 666
Msa0184070 MtrunA17_Chr8g0380511 59.897 389 145 7 53 439 36 415 1.68e-172 489
Msa0184070 MtrunA17_Chr2g0309251 56.331 387 164 4 53 439 44 425 8.76e-166 473
Msa0184070 MtrunA17_Chr8g0368161 58.056 391 139 5 50 439 81 447 5.43e-162 464
Msa0184070 MtrunA17_Chr4g0058771 56.359 401 152 6 39 439 103 480 5.53e-161 462
Msa0184070 MtrunA17_Chr3g0117271 57.033 391 144 7 49 439 129 495 3.76e-157 453
Msa0184070 MtrunA17_Chr5g0413131 53.882 425 171 7 16 439 62 462 1.22e-155 448
Msa0184070 MtrunA17_Chr8g0368241 47.552 429 194 8 17 439 10 413 3.33e-134 392
Msa0184070 MtrunA17_Chr2g0314461 46.734 398 182 7 43 439 37 405 8.92e-125 368
Msa0184070 MtrunA17_Chr2g0314471 44.028 427 200 8 22 439 9 405 2.59e-124 367
Msa0184070 MtrunA17_Chr2g0314451 45.116 430 199 7 18 439 4 404 1.17e-122 362
Msa0184070 MtrunA17_Chr8g0368201 49.080 326 134 7 131 439 9 319 1.92e-103 310
Msa0184070 MtrunA17_Chr6g0453411 42.820 383 190 11 70 439 8 374 2.70e-100 304
Msa0184070 MtrunA17_Chr8g0368291 39.631 434 212 11 17 439 10 404 1.59e-98 300
Msa0184070 MtrunA17_Chr8g0368341 41.351 370 177 8 71 439 4 334 1.67e-92 283
Msa0184070 MtrunA17_Chr3g0135381 38.517 418 234 8 31 437 29 434 2.49e-91 284
Msa0184070 MtrunA17_Chr2g0326371 50.000 302 129 9 142 439 76 359 3.08e-88 273
Msa0184070 MtrunA17_Chr3g0135371 36.674 439 243 10 11 438 18 432 2.91e-86 270
Msa0184070 MtrunA17_Chr8g0368181 59.375 192 77 1 248 439 2 192 1.15e-82 252
Msa0184070 MtrunA17_Chr8g0368221 48.062 258 105 7 58 315 30 258 3.96e-74 233
Msa0184070 MtrunA17_Chr3g0129891 52.284 197 86 3 246 440 190 380 1.49e-72 233
Msa0184070 MtrunA17_Chr3g0129891 40.299 134 61 3 54 180 82 203 1.32e-20 93.2
Msa0184070 MtrunA17_Chr3g0135401 39.879 331 174 8 123 437 26 347 6.17e-70 225
Msa0184070 MtrunA17_Chr1g0151871 37.963 324 171 7 120 439 29 326 2.94e-69 223
Msa0184070 MtrunA17_Chr8g0368331 39.568 278 134 5 191 439 13 285 1.52e-67 217
Msa0184070 MtrunA17_Chr8g0368361 46.392 194 101 2 248 439 2 194 1.36e-66 211
Msa0184070 MtrunA17_Chr8g0368281 47.668 193 100 1 248 439 2 194 6.00e-64 204
Msa0184070 MtrunA17_Chr3g0138811 29.496 417 242 13 26 436 16 386 2.87e-49 172
Msa0184070 MtrunA17_Chr8g0368231 57.258 124 53 0 316 439 8 131 1.81e-48 162
Msa0184070 MtrunA17_Chr3g0135441 37.083 240 128 4 200 437 10 228 2.88e-47 162
Msa0184070 MtrunA17_Chr3g0135431 30.155 388 186 9 17 336 16 386 1.88e-43 157
Msa0184070 MtrunA17_Chr2g0302691 32.982 379 195 15 68 434 37 368 5.56e-43 155
Msa0184070 MtrunA17_Chr8g0368351 40.278 216 99 6 34 249 63 248 2.70e-42 150
Msa0184070 MtrunA17_Chr8g0368261 51.587 126 59 2 315 439 4 128 1.50e-40 141
Msa0184070 MtrunA17_Chr8g0368381 50.442 113 56 0 248 360 2 114 9.89e-40 139
Msa0184070 MtrunA17_Chr8g0339871 37.200 250 137 11 202 440 2 242 5.89e-39 140
Msa0184070 MtrunA17_Chr8g0391201 35.983 239 138 9 204 434 4 235 4.07e-36 133
Msa0184070 MtrunA17_Chr5g0445181 34.050 279 141 11 98 375 61 297 1.06e-34 130
Msa0184070 MtrunA17_Chr8g0368171 30.672 238 117 7 17 249 10 204 1.08e-24 100
Msa0184070 MtrunA17_Chr2g0302651 33.937 221 110 10 225 434 1 196 1.90e-23 97.4
Msa0184070 MtrunA17_Chr3g0135451 37.107 159 85 4 17 167 8 159 2.80e-23 97.1
Msa0184070 MtrunA17_Chr8g0353941 32.447 188 117 5 253 436 1 182 9.33e-23 95.1
Msa0184070 MtrunA17_Chr8g0353931 31.878 229 119 11 30 249 3 203 2.64e-22 94.4
Msa0184070 MtrunA17_Chr8g0353971 33.918 171 105 4 268 434 4 170 1.27e-21 91.7
Msa0184070 MtrunA17_Chr8g0368321 42.105 114 49 3 54 167 46 142 3.75e-21 90.5
Msa0184070 MtrunA17_Chr8g0368271 41.228 114 55 2 54 167 52 153 3.25e-20 88.6
Msa0184070 MtrunA17_Chr5g0434771 33.962 159 97 5 261 417 12 164 1.68e-19 85.9
Msa0184070 MtrunA17_Chr5g0426971 29.897 194 110 6 200 393 46 213 3.47e-18 84.0
Msa0184070 MtrunA17_Chr8g0353961 34.783 138 74 4 30 161 3 130 1.61e-17 79.7
Msa0184070 MtrunA17_Chr5g0443801 33.898 118 75 2 196 313 15 129 2.87e-16 76.6
Msa0184070 MtrunA17_Chr3g0135411 55.738 61 26 1 42 101 52 112 2.29e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184070 AT5G18460.1 72.030 404 106 4 38 440 33 430 0.0 600
Msa0184070 AT1G55360.1 54.651 430 180 8 11 439 6 421 2.57e-171 487
Msa0184070 AT3G13510.1 54.654 419 173 8 23 439 15 418 1.01e-169 483
Msa0184070 AT5G56530.2 53.721 430 183 7 11 439 5 419 4.99e-167 476
Msa0184070 AT5G56530.1 53.721 430 183 7 11 439 5 419 4.99e-167 476
Msa0184070 AT1G10750.1 58.462 390 138 5 50 439 101 466 3.68e-163 468
Msa0184070 AT1G23340.2 54.374 423 165 9 17 439 14 408 7.08e-163 465
Msa0184070 AT1G23340.1 54.374 423 165 9 17 439 14 408 7.08e-163 465
Msa0184070 AT5G50150.1 55.721 402 154 6 39 439 41 419 1.97e-160 459
Msa0184070 AT1G70550.1 57.179 390 143 8 50 439 99 464 4.26e-158 455
Msa0184070 AT2G44210.1 50.948 422 194 7 19 439 5 414 2.82e-157 451
Msa0184070 AT2G44210.2 50.957 418 168 7 53 439 33 444 6.57e-151 436
Msa0184070 AT2G44220.1 44.681 423 209 7 18 439 4 402 1.08e-133 390
Msa0184070 AT3G48230.1 51.042 384 164 9 58 439 11 372 3.32e-132 386
Msa0184070 AT2G44240.1 43.294 425 211 10 18 439 4 401 1.70e-123 365
Msa0184070 AT2G17750.1 45.063 395 197 9 45 439 21 395 2.31e-123 364
Msa0184070 AT2G19360.1 42.955 440 211 7 19 440 9 426 4.79e-123 364
Msa0184070 AT5G19170.1 43.257 393 191 7 46 434 1 365 2.49e-107 323
Msa0184070 AT2G44250.1 41.121 428 227 10 17 439 1 408 4.36e-107 323
Msa0184070 AT1G23340.3 54.044 272 99 8 44 315 39 284 1.66e-95 289
Msa0184070 AT2G44250.2 42.687 335 176 10 111 439 1 325 9.22e-83 258
Msa0184070 AT5G25950.1 39.000 400 218 10 54 439 44 431 5.35e-81 256
Msa0184070 AT2G20170.1 36.879 423 222 15 22 434 9 396 3.77e-74 238
Msa0184070 AT2G20170.2 36.879 423 222 15 22 434 9 396 3.77e-74 238
Msa0184070 AT5G25960.1 37.949 390 180 10 54 439 25 356 4.21e-70 226
Msa0184070 AT4G23370.1 33.652 419 245 12 25 434 7 401 2.23e-69 226
Msa0184070 AT4G23390.1 36.082 388 206 13 58 434 41 397 1.53e-67 221
Msa0184070 AT2G20170.3 34.434 424 216 15 21 434 8 379 2.05e-64 212
Msa0184070 AT4G23365.1 32.506 403 235 13 41 434 24 398 7.87e-64 211
Msa0184070 AT4G23355.1 32.741 394 232 13 47 434 32 398 2.31e-61 205
Msa0184070 AT1G70550.2 50.230 217 84 8 50 266 99 291 9.54e-61 199
Msa0184070 AT4G23350.1 31.095 402 244 12 41 434 24 400 1.91e-60 202
Msa0184070 AT4G23380.1 32.487 394 222 13 52 434 38 398 3.82e-59 199
Msa0184070 AT4G23360.1 32.506 403 236 14 41 434 24 399 4.68e-59 199
Msa0184070 AT1G10750.2 49.020 204 80 5 50 253 101 280 1.74e-52 180
Msa0184070 AT2G35250.1 34.417 369 182 13 75 436 24 339 1.35e-48 169
Msa0184070 AT4G17505.1 30.933 375 185 12 66 437 18 321 4.07e-45 159
Msa0184070 AT1G10190.1 29.907 428 256 16 19 435 1 395 5.95e-44 158
Msa0184070 AT4G15050.1 30.049 406 242 15 39 435 23 395 6.95e-44 158
Msa0184070 AT2G27320.1 34.579 321 159 10 77 395 30 301 1.34e-42 153
Msa0184070 AT5G46200.1 29.426 401 223 17 77 439 30 408 8.22e-40 147
Msa0184070 AT4G15053.1 30.691 391 232 14 54 435 42 402 1.08e-39 147
Msa0184070 AT4G10220.1 30.233 387 230 15 53 434 47 398 1.02e-38 144
Msa0184070 AT2G24950.1 29.111 450 259 22 6 435 1 410 3.01e-38 143
Msa0184070 AT5G25410.1 27.968 379 224 12 62 434 28 363 3.22e-37 140
Msa0184070 AT5G46810.1 30.108 372 216 15 78 436 11 351 4.96e-37 139
Msa0184070 AT4G23373.1 32.453 265 135 5 25 289 7 227 5.17e-37 137
Msa0184070 AT5G05030.1 29.124 388 218 15 59 436 19 359 5.48e-37 139
Msa0184070 AT5G60380.1 26.984 378 227 13 65 434 31 367 3.43e-36 137
Msa0184070 AT5G46820.1 30.163 368 209 13 78 434 17 347 1.54e-35 135
Msa0184070 AT4G17860.1 28.117 377 205 17 78 439 29 354 4.75e-32 125
Msa0184070 AT4G10210.1 27.358 424 260 16 6 419 1 386 5.46e-30 120
Msa0184070 AT2G38255.1 35.088 228 104 6 78 305 14 197 8.14e-30 115
Msa0184070 AT2G03930.2 56.522 92 40 0 190 281 42 133 3.56e-29 112
Msa0184070 AT4G23080.1 28.894 398 231 17 53 436 47 406 2.00e-28 116
Msa0184070 AT5G11660.1 29.836 305 182 12 139 436 25 304 8.64e-28 112
Msa0184070 AT5G25415.1 29.825 228 141 6 220 436 113 332 1.10e-21 95.9
Msa0184070 AT5G36680.1 27.653 311 186 13 134 434 46 327 3.85e-21 94.4
Msa0184070 AT5G36770.1 27.653 311 186 13 134 434 46 327 3.85e-21 94.4
Msa0184070 AT5G04937.1 29.648 199 113 7 245 436 22 200 3.55e-17 80.1
Msa0184070 AT5G37520.1 32.386 176 102 9 265 434 33 197 5.13e-15 73.9

Find 174 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAAATTTGGATTCTTTC+TGG 0.084366 1_4:+73214120 Msa0184070:CDS
AATTTAGTCTTTCTCAAATT+TGG 0.102248 1_4:+73214109 Msa0184070:CDS
CATTGTTTGGTTTATTTGTT+TGG 0.151929 1_4:+73212287 Msa0184070:five_prime_UTR
TGTCTTAAATATTGTATTAA+TGG 0.166742 1_4:-73212178 None:intergenic
GGTTTATTTGTTTGGGTATA+TGG 0.197181 1_4:+73212295 Msa0184070:five_prime_UTR
GCTAAATCTTTGTATGAATA+TGG 0.199278 1_4:+73213593 Msa0184070:CDS
CTCTAATAATTGTTTGTAAA+AGG 0.210392 1_4:-73212508 None:intergenic
TGGATTCTTTCTGGATCTTT+TGG 0.225879 1_4:+73214129 Msa0184070:CDS
TCGCCGTTATTAACAACTTC+AGG 0.268540 1_4:-73213662 None:intergenic
TGTTTGGGTATATGGTAAAA+TGG 0.269322 1_4:+73212303 Msa0184070:five_prime_UTR
TTGGACAACGTGTCCCATTT+TGG 0.271651 1_4:-73213523 None:intergenic
TGGCAAATGTGGCACCAAAA+TGG 0.279621 1_4:+73213509 Msa0184070:CDS
TGGCGTTGAAAGAGAAATTA+TGG 0.285107 1_4:+73212415 Msa0184070:exon
TTTCTTGAGAGGAAGAAAAT+AGG 0.292445 1_4:-73212241 None:intergenic
GACCCAGATTATTCACTTAC+TGG 0.306757 1_4:+73214649 Msa0184070:CDS
GCGATTGCGTTCACAAAATC+AGG 0.309262 1_4:+73214021 Msa0184070:CDS
TTTGGTGACGGCACATTGAT+AGG 0.315027 1_4:+73215133 Msa0184070:CDS
ATTGTTTGGTTTATTTGTTT+GGG 0.331356 1_4:+73212288 Msa0184070:five_prime_UTR
CACTTTGCTGAAGATGGCTT+TGG 0.334222 1_4:+73215277 Msa0184070:CDS
TTTACCTATTTCTTCTCTCT+TGG 0.342556 1_4:+73212395 Msa0184070:five_prime_UTR
TTGGAAAAGCAAGCTACTTC+AGG 0.347824 1_4:+73215296 Msa0184070:CDS
GCCTATACTTCGTGTAGTTT+AGG 0.363508 1_4:-73212544 None:intergenic
TCCGGCCACTTTGCTGAAGA+TGG 0.363622 1_4:+73215271 Msa0184070:CDS
AAATTTGCGGTTGGCATGGC+TGG 0.366473 1_4:-73211952 None:intergenic
CGTCCAGTAAGTGAATAATC+TGG 0.368717 1_4:-73214652 None:intergenic
CAAAGTAAATCTAACTTTCA+TGG 0.373663 1_4:-73212332 None:intergenic
TATACTACATAAGTACTAAC+TGG 0.375399 1_4:-73215501 None:intergenic
ATAAGAAGAGAGAGGAGAAA+TGG 0.388996 1_4:-73214921 None:intergenic
TGCTACAACCTTCTTTGTGC+TGG 0.397788 1_4:+73214860 Msa0184070:CDS
CCAACACACCTCCACTCAAA+TGG 0.398678 1_4:+73215246 Msa0184070:CDS
TAGGTCAGTCCGGAGCTTTA+TGG 0.400753 1_4:+73214618 Msa0184070:intron
AGAGCTCCTACAACAACAAA+TGG 0.403956 1_4:+73215407 Msa0184070:CDS
TTGAATGCATAAAACATGAT+TGG 0.414562 1_4:+73215664 Msa0184070:three_prime_UTR
GCATAAAACATGATTGGAAA+TGG 0.416691 1_4:+73215670 Msa0184070:three_prime_UTR
GTATATGATAGTAGTAATAA+TGG 0.420648 1_4:-73212067 None:intergenic
ATAATATTACCTCTATAGTT+AGG 0.422015 1_4:-73212631 None:intergenic
TCGTGCCACCATGGTGCAAT+GGG 0.425828 1_4:+73215192 Msa0184070:CDS
GGCAAATGTGGCACCAAAAT+GGG 0.428900 1_4:+73213510 Msa0184070:CDS
ATAAACCTTCCAGATGAGTA+TGG 0.431310 1_4:-73214966 None:intergenic
CGCCGTTATTAACAACTTCA+GGG 0.431464 1_4:-73213661 None:intergenic
AAATAAACCAAACAATGAAA+AGG 0.432690 1_4:-73212281 None:intergenic
AAATCAGGGGAAGAGGTGTA+TGG 0.433416 1_4:+73214036 Msa0184070:CDS
GGGAACTGGTGGATGAGATT+TGG 0.437839 1_4:+73215115 Msa0184070:CDS
TGTGCCCCATTTGTTGTTGT+AGG 0.439039 1_4:-73215413 None:intergenic
ATATTCATATCCATTCCCTT+TGG 0.441719 1_4:-73213437 None:intergenic
GGCAGAAACAAGAATGGAAA+TGG 0.447953 1_4:-73215617 None:intergenic
GAGCATTGTGGATTGTTGCC+AGG 0.452796 1_4:-73215454 None:intergenic
TCAAGGTTGCACAAACGTTA+AGG 0.453406 1_4:-73211992 None:intergenic
GAGCTCCTACAACAACAAAT+GGG 0.454693 1_4:+73215408 Msa0184070:CDS
AACGCCAAGAGAGAAGAAAT+AGG 0.456202 1_4:-73212399 None:intergenic
GCTGATTGCATTACTAGTAT+TGG 0.461023 1_4:+73212356 Msa0184070:five_prime_UTR
AGGATCCACAACTTGGGAAC+TGG 0.461610 1_4:+73215101 Msa0184070:intron
ATCGTGCCACCATGGTGCAA+TGG 0.461729 1_4:+73215191 Msa0184070:CDS
AGTGACACATATCAAGCAAC+TGG 0.463842 1_4:+73214836 Msa0184070:CDS
ATTCCCTTTGGCATCGTCGT+TGG 0.464017 1_4:-73213425 None:intergenic
CTAAATCTTTGTATGAATAT+GGG 0.468256 1_4:+73213594 Msa0184070:CDS
AGTGGAGGTGTGTTGGCCAT+TGG 0.481123 1_4:-73215239 None:intergenic
TTTAAAGAATCACAAAATCC+AGG 0.482298 1_4:+73212876 Msa0184070:CDS
TTTATGATGCTAGCTGTTCA+AGG 0.482412 1_4:-73212009 None:intergenic
TTGACATCACCATACTCATC+TGG 0.484373 1_4:+73214957 Msa0184070:CDS
ATTGATTAGGATCCACAACT+TGG 0.486292 1_4:+73215094 Msa0184070:intron
TCATTGACTACTTCCACCGT+TGG 0.487439 1_4:-73214087 None:intergenic
CAACACACCTCCACTCAAAT+GGG 0.487631 1_4:+73215247 Msa0184070:CDS
TAGCATTGAAGCTGGTTGGC+AGG 0.488190 1_4:+73214167 Msa0184070:CDS
AATGGGACACGTTGTCCAAA+GGG 0.489971 1_4:+73213527 Msa0184070:CDS
AATAAACCAAACAATGAAAA+GGG 0.491298 1_4:-73212280 None:intergenic
GATGAGAGAAGTGGTAAGAG+TGG 0.497824 1_4:+73213479 Msa0184070:CDS
AATCAGGGGAAGAGGTGTAT+GGG 0.502251 1_4:+73214037 Msa0184070:CDS
TGAGGAAAGGATGGTGGGTG+AGG 0.502471 1_4:-73212155 None:intergenic
TTAATAGCATTGAAGCTGGT+TGG 0.503786 1_4:+73214163 Msa0184070:CDS
TGCACCGTGCTCCGCCGTAT+CGG 0.505620 1_4:-73213557 None:intergenic
TTGCACCATGGTGGCACGAT+CGG 0.507100 1_4:-73215188 None:intergenic
ATCCACCAGTTCCCAAGTTG+TGG 0.508328 1_4:-73215106 None:intergenic
CTGGGCTAAGGTTAAGATGT+TGG 0.509928 1_4:-73215362 None:intergenic
GTGGTGAACACTCGCGCCAA+TGG 0.510609 1_4:+73215223 Msa0184070:CDS
AGCAAGAAGAAGATGAGGAG+TGG 0.515521 1_4:-73212471 None:intergenic
TTGCACAAACGTTAAGGAGA+TGG 0.515856 1_4:-73211986 None:intergenic
ACCTCCACTCAAATGGGTTC+CGG 0.515882 1_4:+73215253 Msa0184070:CDS
CGTATCGGTACAGTTCCCTT+TGG 0.516871 1_4:-73213542 None:intergenic
ATTACCTCTATAGTTAGGAC+AGG 0.517511 1_4:-73212626 None:intergenic
CGCGGAGGTGAGACTGTTGT+CGG 0.520352 1_4:-73215338 None:intergenic
CATGGTGCAATGGGGTGGCG+AGG 0.521629 1_4:+73215201 Msa0184070:CDS
AATAATTGTTTGTAAAAGGA+AGG 0.522506 1_4:-73212504 None:intergenic
TCTAGAGGAGATGAGAGAAG+TGG 0.524587 1_4:+73213470 Msa0184070:CDS
AACGACGATGCCAAAGGGAA+TGG 0.525654 1_4:+73213427 Msa0184070:CDS
GCACAAAACCAGCACAAAGA+AGG 0.529315 1_4:-73214868 None:intergenic
TTGTTACTTCAGAGTCAAGA+TGG 0.529370 1_4:+73212799 Msa0184070:intron
ACAAATAGCAAGATAGCCAT+TGG 0.530075 1_4:+73214893 Msa0184070:CDS
TGCACAAACGTTAAGGAGAT+GGG 0.532098 1_4:-73211985 None:intergenic
GCCATCTTCAGCAAAGTGGC+CGG 0.534756 1_4:-73215272 None:intergenic
GAAAGAGAAATAGAAAGAGA+AGG 0.537513 1_4:+73212207 Msa0184070:five_prime_UTR
TTAGCTAAGAGAGACCCTCT+TGG 0.539344 1_4:-73212120 None:intergenic
ATTAATACAATATTTAAGAC+AGG 0.539849 1_4:+73212180 Msa0184070:five_prime_UTR
CTGCTGTCACCATAAAGCTC+CGG 0.539948 1_4:-73214627 None:intergenic
AAGAAAGCAAGAAGAAGATG+AGG 0.541340 1_4:-73212476 None:intergenic
TAAATATTGTATTAATGGTG+AGG 0.541813 1_4:-73212173 None:intergenic
TAATGGTGAGGAAAGGATGG+TGG 0.542775 1_4:-73212161 None:intergenic
TTAGTAGTTTGAGACTTGAA+AGG 0.545306 1_4:+73212570 Msa0184070:CDS
TTGGTGACGGCACATTGATA+GGG 0.548675 1_4:+73215134 Msa0184070:CDS
TTGAGAGGAAGAAAATAGGA+AGG 0.548794 1_4:-73212237 None:intergenic
AGAGAAATTTGCGGTTGGCA+TGG 0.550112 1_4:-73211956 None:intergenic
AGCATTGTGGATTGTTGCCA+GGG 0.551399 1_4:-73215453 None:intergenic
GATCTTAATAGCATTGAAGC+TGG 0.552923 1_4:+73214159 Msa0184070:CDS
TTGCACTAGTTAGGTCAGTC+CGG 0.552965 1_4:+73214608 Msa0184070:intron
ACCTAAACTACACGAAGTAT+AGG 0.555495 1_4:+73212543 Msa0184070:CDS
CAAAGCCATCTTCAGCAAAG+TGG 0.555709 1_4:-73215276 None:intergenic
ACGGCACATTGATAGGGTAC+TGG 0.556765 1_4:+73215140 Msa0184070:CDS
GGTGCAATGGGGTGGCGAGG+TGG 0.560849 1_4:+73215204 Msa0184070:CDS
GTCCAGTAAGTGAATAATCT+GGG 0.564075 1_4:-73214651 None:intergenic
AGGAGAAATGGAAGCTCCAA+TGG 0.564350 1_4:-73214909 None:intergenic
ACAAACAAGAAGGACGGAAG+TGG 0.564408 1_4:-73215638 None:intergenic
AACATGAAGCTAAGATGACT+TGG 0.565165 1_4:-73215552 None:intergenic
TATGGTGATGTCAAATTGCT+TGG 0.565653 1_4:-73214948 None:intergenic
TATTAATGGTGAGGAAAGGA+TGG 0.568483 1_4:-73212164 None:intergenic
CAGATTATTCACTTACTGGA+CGG 0.568692 1_4:+73214653 Msa0184070:CDS
CCATTTGAGTGGAGGTGTGT+TGG 0.568898 1_4:-73215246 None:intergenic
AGGCCAACGACGATGCCAAA+GGG 0.574441 1_4:+73213422 Msa0184070:CDS
AAATGGGACACGTTGTCCAA+AGG 0.575071 1_4:+73213526 Msa0184070:CDS
CGATTGCGTTCACAAAATCA+GGG 0.576262 1_4:+73214022 Msa0184070:CDS
GTTCACAAAATCAGGGGAAG+AGG 0.576386 1_4:+73214029 Msa0184070:CDS
AAGGCCAACGACGATGCCAA+AGG 0.577437 1_4:+73213421 Msa0184070:CDS
ATCCACAACTTGGGAACTGG+TGG 0.577654 1_4:+73215104 Msa0184070:CDS
TTGATTAGGATCCACAACTT+GGG 0.578524 1_4:+73215095 Msa0184070:intron
AAAAGCAACAATAAATGTGT+GGG 0.580116 1_4:+73214062 Msa0184070:CDS
AATAAATGTGTGGGATCCAA+CGG 0.581407 1_4:+73214071 Msa0184070:CDS
ATTGTATTAATGGTGAGGAA+AGG 0.583949 1_4:-73212168 None:intergenic
CAAAAGCAACAATAAATGTG+TGG 0.585339 1_4:+73214061 Msa0184070:CDS
AAGTGTGTGAATAACTCCAC+TGG 0.587412 1_4:-73215163 None:intergenic
GGAAGTGGCAGAAACAAGAA+TGG 0.590112 1_4:-73215623 None:intergenic
AATATTAATGAAGAGTCTAG+AGG 0.592197 1_4:+73213455 Msa0184070:CDS
AGAAAAGAGAAATTTGCGGT+TGG 0.593162 1_4:-73211961 None:intergenic
AAATTGGGGAAATTTCAGCT+TGG 0.593582 1_4:-73215724 None:intergenic
TGTCGGTGTCTACAATCTCA+AGG 0.593806 1_4:-73215321 None:intergenic
ACCTCTATAGTTAGGACAGG+GGG 0.594543 1_4:-73212623 None:intergenic
GGGAAGAAAAGAGAAATTTG+CGG 0.594832 1_4:-73211965 None:intergenic
AATGGTGAGGAAAGGATGGT+GGG 0.599351 1_4:-73212160 None:intergenic
CTCGCCACCCCATTGCACCA+TGG 0.599661 1_4:-73215200 None:intergenic
GCCACCATGGTGCAATGGGG+TGG 0.599972 1_4:+73215196 Msa0184070:CDS
CATTCAATCAACAAACAAGA+AGG 0.601184 1_4:-73215648 None:intergenic
CTTAGCCGATCGTGCCACCA+TGG 0.601641 1_4:+73215183 Msa0184070:CDS
TAAGGTTAAGATGTTGGTCG+CGG 0.602338 1_4:-73215356 None:intergenic
CCGTTATTAACAACTTCAGG+GGG 0.604223 1_4:-73213659 None:intergenic
GAAGTTGTTAATAACGGCGA+AGG 0.604884 1_4:+73213665 Msa0184070:CDS
CAAAGGGAACTGTACCGATA+CGG 0.605213 1_4:+73213543 Msa0184070:CDS
TGAGACTTGAAAGGATTCAG+AGG 0.607933 1_4:+73212579 Msa0184070:CDS
GGTTAAGATGTTGGTCGCGG+AGG 0.610177 1_4:-73215353 None:intergenic
TGGACACTATTATTGTTGAG+AGG 0.613110 1_4:+73212435 Msa0184070:CDS
ATTGATAGGGTACTGGCCAG+TGG 0.614292 1_4:+73215147 Msa0184070:CDS
TGTACCGATACGGCGGAGCA+CGG 0.614700 1_4:+73213553 Msa0184070:CDS
TCGTTGCGACGGAATAAAAG+TGG 0.617469 1_4:-73213635 None:intergenic
TTACCTCTATAGTTAGGACA+GGG 0.618276 1_4:-73212625 None:intergenic
GCCGGAACCCATTTGAGTGG+AGG 0.619376 1_4:-73215254 None:intergenic
CATCACCATACTCATCTGGA+AGG 0.620289 1_4:+73214961 Msa0184070:CDS
CAATCAACAAACAAGAAGGA+CGG 0.625186 1_4:-73215644 None:intergenic
GTGGTGTGGCATGGCAAATG+TGG 0.628794 1_4:+73213498 Msa0184070:CDS
AGTGATGATCACGAGCATTG+TGG 0.630196 1_4:-73215466 None:intergenic
GTGGCCGGAACCCATTTGAG+TGG 0.633212 1_4:-73215257 None:intergenic
ATCAGGGGAAGAGGTGTATG+GGG 0.633285 1_4:+73214038 Msa0184070:CDS
GCCGTTATTAACAACTTCAG+GGG 0.633552 1_4:-73213660 None:intergenic
GTGGTAAGAGTGGTGTGGCA+TGG 0.656278 1_4:+73213489 Msa0184070:CDS
GCCACCCCATTGCACCATGG+TGG 0.657695 1_4:-73215197 None:intergenic
TCATAACATGATAGTCCAAG+AGG 0.660472 1_4:+73212105 Msa0184070:five_prime_UTR
CATAACATGATAGTCCAAGA+GGG 0.666154 1_4:+73212106 Msa0184070:five_prime_UTR
TTGGCATTATAAGAAGAGAG+AGG 0.666431 1_4:-73214929 None:intergenic
GAGAAGTGGTAAGAGTGGTG+TGG 0.676091 1_4:+73213484 Msa0184070:CDS
AGCTCCTACAACAACAAATG+GGG 0.676238 1_4:+73215409 Msa0184070:CDS
AAATGTGTGGGATCCAACGG+TGG 0.688543 1_4:+73214074 Msa0184070:CDS
TAATAACGGCGAAGGCCATG+AGG 0.692332 1_4:+73213673 Msa0184070:CDS
AGGGAACTGTACCGATACGG+CGG 0.708061 1_4:+73213546 Msa0184070:CDS
GATTGCGTTCACAAAATCAG+GGG 0.725289 1_4:+73214023 Msa0184070:CDS
CGTGCCACCATGGTGCAATG+GGG 0.726243 1_4:+73215193 Msa0184070:CDS
TGGTGGATGAGATTTGGTGA+CGG 0.732995 1_4:+73215121 Msa0184070:CDS
TACCTCTATAGTTAGGACAG+GGG 0.733427 1_4:-73212624 None:intergenic
AATATGGGAAGAAACAACGA+CGG 0.747260 1_4:+73213609 Msa0184070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATTTTTTAACAGAAA+AGG + chr1_4:73213402-73213421 Msa0184070:intron 10.0%
!!! TTAGACTATAATATTTTTTT+TGG - chr1_4:73214241-73214260 None:intergenic 10.0%
!!! TAATTAGAGATATATTTTAA+AGG - chr1_4:73214333-73214352 None:intergenic 10.0%
!! TGTCTTAAATATTGTATTAA+TGG - chr1_4:73212181-73212200 None:intergenic 15.0%
!!! ATTAATACAATATTTAAGAC+AGG + chr1_4:73212180-73212199 Msa0184070:five_prime_UTR 15.0%
!! AACAATATATATATATAGCT+AGG - chr1_4:73212726-73212745 None:intergenic 15.0%
!! TAATGAACATATAAGAATAA+AGG - chr1_4:73212755-73212774 None:intergenic 15.0%
!!! ATTTTGTGATTCTTTAAAAA+GGG - chr1_4:73212874-73212893 None:intergenic 15.0%
!!! ATTCATTTGTATTTATTTCT+TGG + chr1_4:73213312-73213331 Msa0184070:intron 15.0%
!!! TTTGCTCTAATTTTTTTTTA+AGG + chr1_4:73213759-73213778 Msa0184070:intron 15.0%
!! TTATATTTATAAATGCGATA+TGG - chr1_4:73213861-73213880 None:intergenic 15.0%
!! TCGCATTTATAAATATAAAT+TGG + chr1_4:73213863-73213882 Msa0184070:intron 15.0%
!! ATATTGTTTAAATAATTGAG+AGG + chr1_4:73213946-73213965 Msa0184070:intron 15.0%
!!! CATTTGATTTTTTATTTGTT+TGG + chr1_4:73214715-73214734 Msa0184070:intron 15.0%
!! GTATATGATAGTAGTAATAA+TGG - chr1_4:73212070-73212089 None:intergenic 20.0%
!! TAAATATTGTATTAATGGTG+AGG - chr1_4:73212176-73212195 None:intergenic 20.0%
!! AATAAACCAAACAATGAAAA+GGG - chr1_4:73212283-73212302 None:intergenic 20.0%
!! AAATAAACCAAACAATGAAA+AGG - chr1_4:73212284-73212303 None:intergenic 20.0%
!!! ATTGTTTGGTTTATTTGTTT+GGG + chr1_4:73212288-73212307 Msa0184070:five_prime_UTR 20.0%
!! AATAATTGTTTGTAAAAGGA+AGG - chr1_4:73212507-73212526 None:intergenic 20.0%
!! CTCTAATAATTGTTTGTAAA+AGG - chr1_4:73212511-73212530 None:intergenic 20.0%
!! ATAATATTACCTCTATAGTT+AGG - chr1_4:73212634-73212653 None:intergenic 20.0%
!!! TTTTGTGATTCTTTAAAAAG+GGG - chr1_4:73212873-73212892 None:intergenic 20.0%
!!! GATTTTGTGATTCTTTAAAA+AGG - chr1_4:73212875-73212894 None:intergenic 20.0%
!! TATATTTCATCACACATATA+TGG + chr1_4:73212901-73212920 Msa0184070:intron 20.0%
!! GGGACAAATTTATAATATTA+AGG - chr1_4:73212996-73213015 None:intergenic 20.0%
!! CAACAGCAAAAAAAAAAAAA+AGG - chr1_4:73213114-73213133 None:intergenic 20.0%
!!! CTTTTTTTTTTTTTTGCTGT+TGG + chr1_4:73213112-73213131 Msa0184070:intron 20.0%
!!! TATTTATTTCTTGGTTTGAA+AGG + chr1_4:73213321-73213340 Msa0184070:intron 20.0%
!! CTAAATCTTTGTATGAATAT+GGG + chr1_4:73213594-73213613 Msa0184070:CDS 20.0%
!!! CTAATTTTTTTTTAAGGCAA+TGG + chr1_4:73213765-73213784 Msa0184070:intron 20.0%
!! AATTGAGAGGAAAATAATTA+AGG + chr1_4:73213959-73213978 Msa0184070:intron 20.0%
!!! AATTTAGTCTTTCTCAAATT+TGG + chr1_4:73214109-73214128 Msa0184070:CDS 20.0%
!!! TATAATCTGAAATTGAAGAT+TGG - chr1_4:73214209-73214228 None:intergenic 20.0%
!!! CATATGCAAAAAGAATTTTT+TGG + chr1_4:73214381-73214400 Msa0184070:intron 20.0%
!!! TGATTTTTTATTTGTTTGGT+TGG + chr1_4:73214719-73214738 Msa0184070:intron 20.0%
!!! GATTTTTTATTTGTTTGGTT+GGG + chr1_4:73214720-73214739 Msa0184070:intron 20.0%
!!! TTGTCAATTACTTATTGATT+AGG + chr1_4:73215081-73215100 Msa0184070:intron 20.0%
!! CATTGTTTGGTTTATTTGTT+TGG + chr1_4:73212287-73212306 Msa0184070:five_prime_UTR 25.0%
! CAAAGTAAATCTAACTTTCA+TGG - chr1_4:73212335-73212354 None:intergenic 25.0%
! TTTAAAGAATCACAAAATCC+AGG + chr1_4:73212876-73212895 Msa0184070:CDS 25.0%
!!! TTTTTTTTGCTGTTGGTTTT+TGG + chr1_4:73213119-73213138 Msa0184070:intron 25.0%
!! ACAAGTTAGTGACATTATTT+TGG - chr1_4:73213205-73213224 None:intergenic 25.0%
! AATATTAATGAAGAGTCTAG+AGG + chr1_4:73213455-73213474 Msa0184070:CDS 25.0%
! GCTAAATCTTTGTATGAATA+TGG + chr1_4:73213593-73213612 Msa0184070:CDS 25.0%
! TAATAATAATACACACCTCA+TGG - chr1_4:73213691-73213710 None:intergenic 25.0%
! TAACTACTATCCATTTATGT+TGG + chr1_4:73213817-73213836 Msa0184070:intron 25.0%
! AAAAGCAACAATAAATGTGT+GGG + chr1_4:73214062-73214081 Msa0184070:CDS 25.0%
!!! TTTTGATAATTGCACTAGTT+AGG + chr1_4:73214599-73214618 Msa0184070:intron 25.0%
!! ATTGTAACAGTTTTTGTTCT+GGG - chr1_4:73215383-73215402 None:intergenic 25.0%
!! TATTGTAACAGTTTTTGTTC+TGG - chr1_4:73215384-73215403 None:intergenic 25.0%
! TATACTACATAAGTACTAAC+TGG - chr1_4:73215504-73215523 None:intergenic 25.0%
! TTGAATGCATAAAACATGAT+TGG + chr1_4:73215664-73215683 Msa0184070:three_prime_UTR 25.0%
ATTGTATTAATGGTGAGGAA+AGG - chr1_4:73212171-73212190 None:intergenic 30.0%
GAAAGAGAAATAGAAAGAGA+AGG + chr1_4:73212207-73212226 Msa0184070:five_prime_UTR 30.0%
TTTCTTGAGAGGAAGAAAAT+AGG - chr1_4:73212244-73212263 None:intergenic 30.0%
! CAATTTCCCTTTTCATTGTT+TGG + chr1_4:73212274-73212293 Msa0184070:five_prime_UTR 30.0%
GGTTTATTTGTTTGGGTATA+TGG + chr1_4:73212295-73212314 Msa0184070:five_prime_UTR 30.0%
TGTTTGGGTATATGGTAAAA+TGG + chr1_4:73212303-73212322 Msa0184070:five_prime_UTR 30.0%
! TTTACCTATTTCTTCTCTCT+TGG + chr1_4:73212395-73212414 Msa0184070:five_prime_UTR 30.0%
! TTAGTAGTTTGAGACTTGAA+AGG + chr1_4:73212570-73212589 Msa0184070:CDS 30.0%
TGTGTGATGAAATATATACC+TGG - chr1_4:73212897-73212916 None:intergenic 30.0%
!! ACTAACTACTCATTTTAGAG+GGG - chr1_4:73213016-73213035 None:intergenic 30.0%
! CACTAACTACTCATTTTAGA+GGG - chr1_4:73213017-73213036 None:intergenic 30.0%
! ACACTAACTACTCATTTTAG+AGG - chr1_4:73213018-73213037 None:intergenic 30.0%
ACTACTTCAACTCATTACAA+AGG - chr1_4:73213148-73213167 None:intergenic 30.0%
ATGTTATATGACGCAGTATA+AGG - chr1_4:73213171-73213190 None:intergenic 30.0%
! GAATAGAAAACGTTTTGCTT+TGG - chr1_4:73213296-73213315 None:intergenic 30.0%
! ATTTCTTGGTTTGAAAGGAA+AGG + chr1_4:73213326-73213345 Msa0184070:intron 30.0%
! TTTCTTGGTTTGAAAGGAAA+GGG + chr1_4:73213327-73213346 Msa0184070:intron 30.0%
AATCCAATTAACTTACTCCT+AGG + chr1_4:73213355-73213374 Msa0184070:intron 30.0%
ATCCAATTAACTTACTCCTA+GGG + chr1_4:73213356-73213375 Msa0184070:intron 30.0%
AACAAAAACTAATCAACCCT+AGG - chr1_4:73213375-73213394 None:intergenic 30.0%
ATATTCATATCCATTCCCTT+TGG - chr1_4:73213440-73213459 None:intergenic 30.0%
! TGGTAGTAATTCTTTTCGTT+TGG + chr1_4:73213883-73213902 Msa0184070:intron 30.0%
CAAAAGCAACAATAAATGTG+TGG + chr1_4:73214061-73214080 Msa0184070:CDS 30.0%
TCTCAAATTTGGATTCTTTC+TGG + chr1_4:73214120-73214139 Msa0184070:CDS 30.0%
AGTAATGTTTCTTCTGAACT+TGG - chr1_4:73214486-73214505 None:intergenic 30.0%
TTCAGAAGAAACATTACTCA+AGG + chr1_4:73214488-73214507 Msa0184070:intron 30.0%
CATTCAATCAACAAACAAGA+AGG - chr1_4:73215651-73215670 None:intergenic 30.0%
GCATAAAACATGATTGGAAA+TGG + chr1_4:73215670-73215689 Msa0184070:three_prime_UTR 30.0%
AGAAAAGAGAAATTTGCGGT+TGG - chr1_4:73211964-73211983 None:intergenic 35.0%
GGGAAGAAAAGAGAAATTTG+CGG - chr1_4:73211968-73211987 None:intergenic 35.0%
TTTATGATGCTAGCTGTTCA+AGG - chr1_4:73212012-73212031 None:intergenic 35.0%
TCATAACATGATAGTCCAAG+AGG + chr1_4:73212105-73212124 Msa0184070:five_prime_UTR 35.0%
CATAACATGATAGTCCAAGA+GGG + chr1_4:73212106-73212125 Msa0184070:five_prime_UTR 35.0%
TATTAATGGTGAGGAAAGGA+TGG - chr1_4:73212167-73212186 None:intergenic 35.0%
TTGAGAGGAAGAAAATAGGA+AGG - chr1_4:73212240-73212259 None:intergenic 35.0%
! GAAAAACGACTTTTCTTGAG+AGG - chr1_4:73212255-73212274 None:intergenic 35.0%
GCTGATTGCATTACTAGTAT+TGG + chr1_4:73212356-73212375 Msa0184070:five_prime_UTR 35.0%
! TGGCGTTGAAAGAGAAATTA+TGG + chr1_4:73212415-73212434 Msa0184070:exon 35.0%
TGGACACTATTATTGTTGAG+AGG + chr1_4:73212435-73212454 Msa0184070:CDS 35.0%
AAGAAAGCAAGAAGAAGATG+AGG - chr1_4:73212479-73212498 None:intergenic 35.0%
ACCTAAACTACACGAAGTAT+AGG + chr1_4:73212543-73212562 Msa0184070:CDS 35.0%
TTACCTCTATAGTTAGGACA+GGG - chr1_4:73212628-73212647 None:intergenic 35.0%
ATTACCTCTATAGTTAGGAC+AGG - chr1_4:73212629-73212648 None:intergenic 35.0%
! TTGTTACTTCAGAGTCAAGA+TGG + chr1_4:73212799-73212818 Msa0184070:intron 35.0%
! CTTTAAAAAGGGGTGATCTA+AGG - chr1_4:73212863-73212882 None:intergenic 35.0%
CATGAAACGCTAGTACAAAA+TGG - chr1_4:73212968-73212987 None:intergenic 35.0%
! CATTTTGTACTAGCGTTTCA+TGG + chr1_4:73212966-73212985 Msa0184070:intron 35.0%
AACCCTAGGAGTAAGTTAAT+TGG - chr1_4:73213361-73213380 None:intergenic 35.0%
AATATGGGAAGAAACAACGA+CGG + chr1_4:73213609-73213628 Msa0184070:CDS 35.0%
! AAGGCAATGGTCTCTATTTT+TGG + chr1_4:73213778-73213797 Msa0184070:intron 35.0%
AAATGCGATATGGACAGTTA+GGG - chr1_4:73213851-73213870 None:intergenic 35.0%
TAAATGCGATATGGACAGTT+AGG - chr1_4:73213852-73213871 None:intergenic 35.0%
!! AAGGAACTAATTTGCTTGAG+TGG + chr1_4:73213978-73213997 Msa0184070:intron 35.0%
AATAAATGTGTGGGATCCAA+CGG + chr1_4:73214071-73214090 Msa0184070:CDS 35.0%
! TGGATTCTTTCTGGATCTTT+TGG + chr1_4:73214129-73214148 Msa0184070:CDS 35.0%
! ATTCTTTCTGGATCTTTTGG+TGG + chr1_4:73214132-73214151 Msa0184070:CDS 35.0%
! GATCTTAATAGCATTGAAGC+TGG + chr1_4:73214159-73214178 Msa0184070:CDS 35.0%
! TTAATAGCATTGAAGCTGGT+TGG + chr1_4:73214163-73214182 Msa0184070:CDS 35.0%
GTCCAGTAAGTGAATAATCT+GGG - chr1_4:73214654-73214673 None:intergenic 35.0%
CAGATTATTCACTTACTGGA+CGG + chr1_4:73214653-73214672 Msa0184070:CDS 35.0%
ACAAATAGCAAGATAGCCAT+TGG + chr1_4:73214893-73214912 Msa0184070:CDS 35.0%
ATAAGAAGAGAGAGGAGAAA+TGG - chr1_4:73214924-73214943 None:intergenic 35.0%
TTGGCATTATAAGAAGAGAG+AGG - chr1_4:73214932-73214951 None:intergenic 35.0%
! TATGGTGATGTCAAATTGCT+TGG - chr1_4:73214951-73214970 None:intergenic 35.0%
ATAAACCTTCCAGATGAGTA+TGG - chr1_4:73214969-73214988 None:intergenic 35.0%
ATTGATTAGGATCCACAACT+TGG + chr1_4:73215094-73215113 Msa0184070:intron 35.0%
TTGATTAGGATCCACAACTT+GGG + chr1_4:73215095-73215114 Msa0184070:intron 35.0%
AACATGAAGCTAAGATGACT+TGG - chr1_4:73215555-73215574 None:intergenic 35.0%
CAATCAACAAACAAGAAGGA+CGG - chr1_4:73215647-73215666 None:intergenic 35.0%
TGCACAAACGTTAAGGAGAT+GGG - chr1_4:73211988-73212007 None:intergenic 40.0%
TTGCACAAACGTTAAGGAGA+TGG - chr1_4:73211989-73212008 None:intergenic 40.0%
TCAAGGTTGCACAAACGTTA+AGG - chr1_4:73211995-73212014 None:intergenic 40.0%
AACGCCAAGAGAGAAGAAAT+AGG - chr1_4:73212402-73212421 None:intergenic 40.0%
GCCTATACTTCGTGTAGTTT+AGG - chr1_4:73212547-73212566 None:intergenic 40.0%
TGAGACTTGAAAGGATTCAG+AGG + chr1_4:73212579-73212598 Msa0184070:CDS 40.0%
TACCTCTATAGTTAGGACAG+GGG - chr1_4:73212627-73212646 None:intergenic 40.0%
CCGTTATTAACAACTTCAGG+GGG - chr1_4:73213662-73213681 None:intergenic 40.0%
CCCCCTGAAGTTGTTAATAA+CGG + chr1_4:73213659-73213678 Msa0184070:CDS 40.0%
GCCGTTATTAACAACTTCAG+GGG - chr1_4:73213663-73213682 None:intergenic 40.0%
CGCCGTTATTAACAACTTCA+GGG - chr1_4:73213664-73213683 None:intergenic 40.0%
TCGCCGTTATTAACAACTTC+AGG - chr1_4:73213665-73213684 None:intergenic 40.0%
GAAGTTGTTAATAACGGCGA+AGG + chr1_4:73213665-73213684 Msa0184070:CDS 40.0%
GGACATAGGACCAACATAAA+TGG - chr1_4:73213830-73213849 None:intergenic 40.0%
ATATGGACAGTTAGGGACAT+AGG - chr1_4:73213844-73213863 None:intergenic 40.0%
CGATTGCGTTCACAAAATCA+GGG + chr1_4:73214022-73214041 Msa0184070:CDS 40.0%
GATTGCGTTCACAAAATCAG+GGG + chr1_4:73214023-73214042 Msa0184070:CDS 40.0%
GACCCAGATTATTCACTTAC+TGG + chr1_4:73214649-73214668 Msa0184070:CDS 40.0%
CGTCCAGTAAGTGAATAATC+TGG - chr1_4:73214655-73214674 None:intergenic 40.0%
AGTGACACATATCAAGCAAC+TGG + chr1_4:73214836-73214855 Msa0184070:CDS 40.0%
TTGACATCACCATACTCATC+TGG + chr1_4:73214957-73214976 Msa0184070:CDS 40.0%
AAGTGTGTGAATAACTCCAC+TGG - chr1_4:73215166-73215185 None:intergenic 40.0%
TTGGAAAAGCAAGCTACTTC+AGG + chr1_4:73215296-73215315 Msa0184070:CDS 40.0%
TAAGGTTAAGATGTTGGTCG+CGG - chr1_4:73215359-73215378 None:intergenic 40.0%
!! ACAGTTTTTGTTCTGGGCTA+AGG - chr1_4:73215377-73215396 None:intergenic 40.0%
AGAGCTCCTACAACAACAAA+TGG + chr1_4:73215407-73215426 Msa0184070:CDS 40.0%
GAGCTCCTACAACAACAAAT+GGG + chr1_4:73215408-73215427 Msa0184070:CDS 40.0%
AGCTCCTACAACAACAAATG+GGG + chr1_4:73215409-73215428 Msa0184070:CDS 40.0%
! TGGGGCACATACTTTTACTA+TGG + chr1_4:73215427-73215446 Msa0184070:CDS 40.0%
! GGCACATACTTTTACTATGG+TGG + chr1_4:73215430-73215449 Msa0184070:CDS 40.0%
GGCAGAAACAAGAATGGAAA+TGG - chr1_4:73215620-73215639 None:intergenic 40.0%
AGAGAAATTTGCGGTTGGCA+TGG - chr1_4:73211959-73211978 None:intergenic 45.0%
TTAGCTAAGAGAGACCCTCT+TGG - chr1_4:73212123-73212142 None:intergenic 45.0%
AATGGTGAGGAAAGGATGGT+GGG - chr1_4:73212163-73212182 None:intergenic 45.0%
TAATGGTGAGGAAAGGATGG+TGG - chr1_4:73212164-73212183 None:intergenic 45.0%
AGCAAGAAGAAGATGAGGAG+TGG - chr1_4:73212474-73212493 None:intergenic 45.0%
ACCTCTATAGTTAGGACAGG+GGG - chr1_4:73212626-73212645 None:intergenic 45.0%
! AAAAAGGGGTGATCTAAGGC+TGG - chr1_4:73212859-73212878 None:intergenic 45.0%
TCTAGAGGAGATGAGAGAAG+TGG + chr1_4:73213470-73213489 Msa0184070:CDS 45.0%
GATGAGAGAAGTGGTAAGAG+TGG + chr1_4:73213479-73213498 Msa0184070:CDS 45.0%
TGGCAAATGTGGCACCAAAA+TGG + chr1_4:73213509-73213528 Msa0184070:CDS 45.0%
GGCAAATGTGGCACCAAAAT+GGG + chr1_4:73213510-73213529 Msa0184070:CDS 45.0%
! TTGGACAACGTGTCCCATTT+TGG - chr1_4:73213526-73213545 None:intergenic 45.0%
AAATGGGACACGTTGTCCAA+AGG + chr1_4:73213526-73213545 Msa0184070:CDS 45.0%
AATGGGACACGTTGTCCAAA+GGG + chr1_4:73213527-73213546 Msa0184070:CDS 45.0%
! CAAAGGGAACTGTACCGATA+CGG + chr1_4:73213543-73213562 Msa0184070:CDS 45.0%
TCGTTGCGACGGAATAAAAG+TGG - chr1_4:73213638-73213657 None:intergenic 45.0%
GCGATTGCGTTCACAAAATC+AGG + chr1_4:73214021-73214040 Msa0184070:CDS 45.0%
GTTCACAAAATCAGGGGAAG+AGG + chr1_4:73214029-73214048 Msa0184070:CDS 45.0%
! AAATCAGGGGAAGAGGTGTA+TGG + chr1_4:73214036-73214055 Msa0184070:CDS 45.0%
! AATCAGGGGAAGAGGTGTAT+GGG + chr1_4:73214037-73214056 Msa0184070:CDS 45.0%
! TCATTGACTACTTCCACCGT+TGG - chr1_4:73214090-73214109 None:intergenic 45.0%
TTGCACTAGTTAGGTCAGTC+CGG + chr1_4:73214608-73214627 Msa0184070:intron 45.0%
TGCTACAACCTTCTTTGTGC+TGG + chr1_4:73214860-73214879 Msa0184070:CDS 45.0%
GCACAAAACCAGCACAAAGA+AGG - chr1_4:73214871-73214890 None:intergenic 45.0%
AGGAGAAATGGAAGCTCCAA+TGG - chr1_4:73214912-73214931 None:intergenic 45.0%
CATCACCATACTCATCTGGA+AGG + chr1_4:73214961-73214980 Msa0184070:CDS 45.0%
!! TGGTGGATGAGATTTGGTGA+CGG + chr1_4:73215121-73215140 Msa0184070:CDS 45.0%
!! TTTGGTGACGGCACATTGAT+AGG + chr1_4:73215133-73215152 Msa0184070:CDS 45.0%
!! TTGGTGACGGCACATTGATA+GGG + chr1_4:73215134-73215153 Msa0184070:CDS 45.0%
CAACACACCTCCACTCAAAT+GGG + chr1_4:73215247-73215266 Msa0184070:CDS 45.0%
CAAAGCCATCTTCAGCAAAG+TGG - chr1_4:73215279-73215298 None:intergenic 45.0%
CACTTTGCTGAAGATGGCTT+TGG + chr1_4:73215277-73215296 Msa0184070:CDS 45.0%
! TGTCGGTGTCTACAATCTCA+AGG - chr1_4:73215324-73215343 None:intergenic 45.0%
CTGGGCTAAGGTTAAGATGT+TGG - chr1_4:73215365-73215384 None:intergenic 45.0%
!! TGTGCCCCATTTGTTGTTGT+AGG - chr1_4:73215416-73215435 None:intergenic 45.0%
! TACTTTTACTATGGTGGCCC+TGG + chr1_4:73215436-73215455 Msa0184070:CDS 45.0%
! AGCATTGTGGATTGTTGCCA+GGG - chr1_4:73215456-73215475 None:intergenic 45.0%
! AGTGATGATCACGAGCATTG+TGG - chr1_4:73215469-73215488 None:intergenic 45.0%
GGAAGTGGCAGAAACAAGAA+TGG - chr1_4:73215626-73215645 None:intergenic 45.0%
ACAAACAAGAAGGACGGAAG+TGG - chr1_4:73215641-73215660 None:intergenic 45.0%
!!! TAATTTTTATAAGAATTAAT+TGG - chr1_4:73212659-73212678 None:intergenic 5.0%
AAATTTGCGGTTGGCATGGC+TGG - chr1_4:73211955-73211974 None:intergenic 50.0%
ATTCCCTTTGGCATCGTCGT+TGG - chr1_4:73213428-73213447 None:intergenic 50.0%
AACGACGATGCCAAAGGGAA+TGG + chr1_4:73213427-73213446 Msa0184070:CDS 50.0%
GAGAAGTGGTAAGAGTGGTG+TGG + chr1_4:73213484-73213503 Msa0184070:CDS 50.0%
! CGTATCGGTACAGTTCCCTT+TGG - chr1_4:73213545-73213564 None:intergenic 50.0%
TAATAACGGCGAAGGCCATG+AGG + chr1_4:73213673-73213692 Msa0184070:CDS 50.0%
! ATCAGGGGAAGAGGTGTATG+GGG + chr1_4:73214038-73214057 Msa0184070:CDS 50.0%
AAATGTGTGGGATCCAACGG+TGG + chr1_4:73214074-73214093 Msa0184070:CDS 50.0%
! TAGCATTGAAGCTGGTTGGC+AGG + chr1_4:73214167-73214186 Msa0184070:CDS 50.0%
TAGGTCAGTCCGGAGCTTTA+TGG + chr1_4:73214618-73214637 Msa0184070:intron 50.0%
CTGCTGTCACCATAAAGCTC+CGG - chr1_4:73214630-73214649 None:intergenic 50.0%
AGGATCCACAACTTGGGAAC+TGG + chr1_4:73215101-73215120 Msa0184070:intron 50.0%
ATCCACAACTTGGGAACTGG+TGG + chr1_4:73215104-73215123 Msa0184070:CDS 50.0%
ATCCACCAGTTCCCAAGTTG+TGG - chr1_4:73215109-73215128 None:intergenic 50.0%
! GGGAACTGGTGGATGAGATT+TGG + chr1_4:73215115-73215134 Msa0184070:CDS 50.0%
ACGGCACATTGATAGGGTAC+TGG + chr1_4:73215140-73215159 Msa0184070:CDS 50.0%
!! ATTGATAGGGTACTGGCCAG+TGG + chr1_4:73215147-73215166 Msa0184070:CDS 50.0%
! CCATTTGAGTGGAGGTGTGT+TGG - chr1_4:73215249-73215268 None:intergenic 50.0%
CCAACACACCTCCACTCAAA+TGG + chr1_4:73215246-73215265 Msa0184070:CDS 50.0%
! ACCTCCACTCAAATGGGTTC+CGG + chr1_4:73215253-73215272 Msa0184070:CDS 50.0%
! GAGCATTGTGGATTGTTGCC+AGG - chr1_4:73215457-73215476 None:intergenic 50.0%
!! TGAGGAAAGGATGGTGGGTG+AGG - chr1_4:73212158-73212177 None:intergenic 55.0%
GCCCCCTGTCCTAACTATAG+AGG + chr1_4:73212622-73212641 Msa0184070:CDS 55.0%
AAGGCCAACGACGATGCCAA+AGG + chr1_4:73213421-73213440 Msa0184070:CDS 55.0%
AGGCCAACGACGATGCCAAA+GGG + chr1_4:73213422-73213441 Msa0184070:CDS 55.0%
GTGGTAAGAGTGGTGTGGCA+TGG + chr1_4:73213489-73213508 Msa0184070:CDS 55.0%
GTGGTGTGGCATGGCAAATG+TGG + chr1_4:73213498-73213517 Msa0184070:CDS 55.0%
! AGGGAACTGTACCGATACGG+CGG + chr1_4:73213546-73213565 Msa0184070:CDS 55.0%
TTGCACCATGGTGGCACGAT+CGG - chr1_4:73215191-73215210 None:intergenic 55.0%
ATCGTGCCACCATGGTGCAA+TGG + chr1_4:73215191-73215210 Msa0184070:CDS 55.0%
TCGTGCCACCATGGTGCAAT+GGG + chr1_4:73215192-73215211 Msa0184070:CDS 55.0%
! AGTGGAGGTGTGTTGGCCAT+TGG - chr1_4:73215242-73215261 None:intergenic 55.0%
! TCCGGCCACTTTGCTGAAGA+TGG + chr1_4:73215271-73215290 Msa0184070:CDS 55.0%
GCCATCTTCAGCAAAGTGGC+CGG - chr1_4:73215275-73215294 None:intergenic 55.0%
GGTTAAGATGTTGGTCGCGG+AGG - chr1_4:73215356-73215375 None:intergenic 55.0%
! TGTACCGATACGGCGGAGCA+CGG + chr1_4:73213553-73213572 Msa0184070:CDS 60.0%
CTTAGCCGATCGTGCCACCA+TGG + chr1_4:73215183-73215202 Msa0184070:CDS 60.0%
CGTGCCACCATGGTGCAATG+GGG + chr1_4:73215193-73215212 Msa0184070:CDS 60.0%
GTGGTGAACACTCGCGCCAA+TGG + chr1_4:73215223-73215242 Msa0184070:CDS 60.0%
GCCGGAACCCATTTGAGTGG+AGG - chr1_4:73215257-73215276 None:intergenic 60.0%
GTGGCCGGAACCCATTTGAG+TGG - chr1_4:73215260-73215279 None:intergenic 60.0%
CGCGGAGGTGAGACTGTTGT+CGG - chr1_4:73215341-73215360 None:intergenic 60.0%
TGCACCGTGCTCCGCCGTAT+CGG - chr1_4:73213560-73213579 None:intergenic 65.0%
CTTCAGGGGGCTCGTTGCGA+CGG - chr1_4:73213649-73213668 None:intergenic 65.0%
GCCACCATGGTGCAATGGGG+TGG + chr1_4:73215196-73215215 Msa0184070:CDS 65.0%
GCCACCCCATTGCACCATGG+TGG - chr1_4:73215200-73215219 None:intergenic 65.0%
CTCGCCACCCCATTGCACCA+TGG - chr1_4:73215203-73215222 None:intergenic 65.0%
CATGGTGCAATGGGGTGGCG+AGG + chr1_4:73215201-73215220 Msa0184070:CDS 65.0%
GGTGCAATGGGGTGGCGAGG+TGG + chr1_4:73215204-73215223 Msa0184070:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 73211934 73215739 73211934 ID=Msa0184070;Name=Msa0184070
chr1_4 mRNA 73211934 73215739 73211934 ID=Msa0184070-mRNA-1;Parent=Msa0184070;Name=Msa0184070-mRNA-1;_AED=0.08;_eAED=0.08;_QI=500|0.83|0.85|1|1|1|7|259|440
chr1_4 exon 73211934 73212643 73211934 ID=Msa0184070-mRNA-1:exon:24843;Parent=Msa0184070-mRNA-1
chr1_4 exon 73212811 73212897 73212811 ID=Msa0184070-mRNA-1:exon:24844;Parent=Msa0184070-mRNA-1
chr1_4 exon 73213419 73213694 73213419 ID=Msa0184070-mRNA-1:exon:24845;Parent=Msa0184070-mRNA-1
chr1_4 exon 73214018 73214188 73214018 ID=Msa0184070-mRNA-1:exon:24846;Parent=Msa0184070-mRNA-1
chr1_4 exon 73214621 73214674 73214621 ID=Msa0184070-mRNA-1:exon:24847;Parent=Msa0184070-mRNA-1
chr1_4 exon 73214836 73214982 73214836 ID=Msa0184070-mRNA-1:exon:24848;Parent=Msa0184070-mRNA-1
chr1_4 exon 73215103 73215739 73215103 ID=Msa0184070-mRNA-1:exon:24849;Parent=Msa0184070-mRNA-1
chr1_4 five_prime_UTR 73211934 73212433 73211934 ID=Msa0184070-mRNA-1:five_prime_utr;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73212434 73212643 73212434 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73212811 73212897 73212811 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73213419 73213694 73213419 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73214018 73214188 73214018 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73214621 73214674 73214621 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73214836 73214982 73214836 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 CDS 73215103 73215480 73215103 ID=Msa0184070-mRNA-1:cds;Parent=Msa0184070-mRNA-1
chr1_4 three_prime_UTR 73215481 73215739 73215481 ID=Msa0184070-mRNA-1:three_prime_utr;Parent=Msa0184070-mRNA-1
Gene Sequence

>Msa0184070

ATGGACACTATTATTGTTGAGAGGAAGAAAAGAATTTCCACTCCTCATCTTCTTCTTGCTTTCTTGTTCACCTTCCTTTTACAAACAATTATTAGAGTTGAATGCATCAACCTAAACTACACGAAGTATAGGCAAGTTAGTAGTTTGAGACTTGAAAGGATTCAGAGGCACTTGAACAAGATTAACAAGCCCCCTGTCCTAACTATAGAGAGTCAAGATGGTGATCTCATAGATTGTGTTCACAAAAGAAAGCAACCAGCCTTAGATCACCCCTTTTTAAAGAATCACAAAATCCAGAAAAGGCCAACGACGATGCCAAAGGGAATGGATATGAATATTAATGAAGAGTCTAGAGGAGATGAGAGAAGTGGTAAGAGTGGTGTGGCATGGCAAATGTGGCACCAAAATGGGACACGTTGTCCAAAGGGAACTGTACCGATACGGCGGAGCACGGTGCATGATGTGTTGAGAGCTAAATCTTTGTATGAATATGGGAAGAAACAACGACGGTCACCACTTTTATTCCGTCGCAACGAGCCCCCTGAAGTTGTTAATAACGGCGAAGGCCATGAGCATGCGATTGCGTTCACAAAATCAGGGGAAGAGGTGTATGGGGCAAAAGCAACAATAAATGTGTGGGATCCAACGGTGGAAGTAGTCAATGAATTTAGTCTTTCTCAAATTTGGATTCTTTCTGGATCTTTTGGTGGCTCTGATCTTAATAGCATTGAAGCTGGTTGGCAGGTCAGTCCGGAGCTTTATGGTGACAGCAGACCCAGATTATTCACTTACTGGACGAGTGACACATATCAAGCAACTGGATGCTACAACCTTCTTTGTGCTGGTTTTGTGCAAACAAATAGCAAGATAGCCATTGGAGCTTCCATTTCTCCTCTCTCTTCTTATAATGCCAAGCAATTTGACATCACCATACTCATCTGGAAGGATCCACAACTTGGGAACTGGTGGATGAGATTTGGTGACGGCACATTGATAGGGTACTGGCCAGTGGAGTTATTCACACACTTAGCCGATCGTGCCACCATGGTGCAATGGGGTGGCGAGGTGGTGAACACTCGCGCCAATGGCCAACACACCTCCACTCAAATGGGTTCCGGCCACTTTGCTGAAGATGGCTTTGGAAAAGCAAGCTACTTCAGGAACCTTGAGATTGTAGACACCGACAACAGTCTCACCTCCGCGACCAACATCTTAACCTTAGCCCAGAACAAAAACTGTTACAATATCAAGAGCTCCTACAACAACAAATGGGGCACATACTTTTACTATGGTGGCCCTGGCAACAATCCACAATGCTCGTGA
Protein sequence

>Msa0184070

MDTIIVERKKRISTPHLLLAFLFTFLLQTIIRVECINLNYTKYRQVSSLRLERIQRHLNKINKPPVLTIESQDGDLIDCVHKRKQPALDHPFLKNHKIQKRPTTMPKGMDMNINEESRGDERSGKSGVAWQMWHQNGTRCPKGTVPIRRSTVHDVLRAKSLYEYGKKQRRSPLLFRRNEPPEVVNNGEGHEHAIAFTKSGEEVYGAKATINVWDPTVEVVNEFSLSQIWILSGSFGGSDLNSIEAGWQVSPELYGDSRPRLFTYWTSDTYQATGCYNLLCAGFVQTNSKIAIGASISPLSSYNAKQFDITILIWKDPQLGNWWMRFGDGTLIGYWPVELFTHLADRATMVQWGGEVVNTRANGQHTSTQMGSGHFAEDGFGKASYFRNLEIVDTDNSLTSATNILTLAQNKNCYNIKSSYNNKWGTYFYYGGPGNNPQCS*