AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1129920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1129920 MtrunA17_Chr7g0274951 97.955 440 9 0 1 440 1 440 0.0 902
Msa1129920 MtrunA17_Chr1g0189961 71.556 450 111 6 5 439 3 450 0.0 666
Msa1129920 MtrunA17_Chr8g0380511 59.897 389 145 7 53 439 36 415 1.68e-172 489
Msa1129920 MtrunA17_Chr2g0309251 56.331 387 164 4 53 439 44 425 8.76e-166 473
Msa1129920 MtrunA17_Chr8g0368161 58.056 391 139 5 50 439 81 447 5.43e-162 464
Msa1129920 MtrunA17_Chr4g0058771 56.359 401 152 6 39 439 103 480 5.53e-161 462
Msa1129920 MtrunA17_Chr3g0117271 57.033 391 144 7 49 439 129 495 3.76e-157 453
Msa1129920 MtrunA17_Chr5g0413131 53.882 425 171 7 16 439 62 462 1.22e-155 448
Msa1129920 MtrunA17_Chr8g0368241 47.552 429 194 8 17 439 10 413 3.33e-134 392
Msa1129920 MtrunA17_Chr2g0314461 46.734 398 182 7 43 439 37 405 8.92e-125 368
Msa1129920 MtrunA17_Chr2g0314471 44.028 427 200 8 22 439 9 405 2.59e-124 367
Msa1129920 MtrunA17_Chr2g0314451 45.116 430 199 7 18 439 4 404 1.17e-122 362
Msa1129920 MtrunA17_Chr8g0368201 49.080 326 134 7 131 439 9 319 1.92e-103 310
Msa1129920 MtrunA17_Chr6g0453411 42.820 383 190 11 70 439 8 374 2.70e-100 304
Msa1129920 MtrunA17_Chr8g0368291 39.631 434 212 11 17 439 10 404 1.59e-98 300
Msa1129920 MtrunA17_Chr8g0368341 41.351 370 177 8 71 439 4 334 1.67e-92 283
Msa1129920 MtrunA17_Chr3g0135381 38.517 418 234 8 31 437 29 434 2.49e-91 284
Msa1129920 MtrunA17_Chr2g0326371 50.000 302 129 9 142 439 76 359 3.08e-88 273
Msa1129920 MtrunA17_Chr3g0135371 36.674 439 243 10 11 438 18 432 2.91e-86 270
Msa1129920 MtrunA17_Chr8g0368181 59.375 192 77 1 248 439 2 192 1.15e-82 252
Msa1129920 MtrunA17_Chr8g0368221 48.062 258 105 7 58 315 30 258 3.96e-74 233
Msa1129920 MtrunA17_Chr3g0129891 52.284 197 86 3 246 440 190 380 1.49e-72 233
Msa1129920 MtrunA17_Chr3g0129891 40.299 134 61 3 54 180 82 203 1.32e-20 93.2
Msa1129920 MtrunA17_Chr3g0135401 39.879 331 174 8 123 437 26 347 6.17e-70 225
Msa1129920 MtrunA17_Chr1g0151871 37.963 324 171 7 120 439 29 326 2.94e-69 223
Msa1129920 MtrunA17_Chr8g0368331 39.568 278 134 5 191 439 13 285 1.52e-67 217
Msa1129920 MtrunA17_Chr8g0368361 46.392 194 101 2 248 439 2 194 1.36e-66 211
Msa1129920 MtrunA17_Chr8g0368281 47.668 193 100 1 248 439 2 194 6.00e-64 204
Msa1129920 MtrunA17_Chr3g0138811 29.496 417 242 13 26 436 16 386 2.87e-49 172
Msa1129920 MtrunA17_Chr8g0368231 57.258 124 53 0 316 439 8 131 1.81e-48 162
Msa1129920 MtrunA17_Chr3g0135441 37.083 240 128 4 200 437 10 228 2.88e-47 162
Msa1129920 MtrunA17_Chr3g0135431 30.155 388 186 9 17 336 16 386 1.88e-43 157
Msa1129920 MtrunA17_Chr2g0302691 32.982 379 195 15 68 434 37 368 5.56e-43 155
Msa1129920 MtrunA17_Chr8g0368351 40.278 216 99 6 34 249 63 248 2.70e-42 150
Msa1129920 MtrunA17_Chr8g0368261 51.587 126 59 2 315 439 4 128 1.50e-40 141
Msa1129920 MtrunA17_Chr8g0368381 50.442 113 56 0 248 360 2 114 9.89e-40 139
Msa1129920 MtrunA17_Chr8g0339871 37.200 250 137 11 202 440 2 242 5.89e-39 140
Msa1129920 MtrunA17_Chr8g0391201 35.983 239 138 9 204 434 4 235 4.07e-36 133
Msa1129920 MtrunA17_Chr5g0445181 34.050 279 141 11 98 375 61 297 1.06e-34 130
Msa1129920 MtrunA17_Chr8g0368171 30.672 238 117 7 17 249 10 204 1.08e-24 100
Msa1129920 MtrunA17_Chr2g0302651 33.937 221 110 10 225 434 1 196 1.90e-23 97.4
Msa1129920 MtrunA17_Chr3g0135451 37.107 159 85 4 17 167 8 159 2.80e-23 97.1
Msa1129920 MtrunA17_Chr8g0353941 32.447 188 117 5 253 436 1 182 9.33e-23 95.1
Msa1129920 MtrunA17_Chr8g0353931 31.878 229 119 11 30 249 3 203 2.64e-22 94.4
Msa1129920 MtrunA17_Chr8g0353971 33.918 171 105 4 268 434 4 170 1.27e-21 91.7
Msa1129920 MtrunA17_Chr8g0368321 42.105 114 49 3 54 167 46 142 3.75e-21 90.5
Msa1129920 MtrunA17_Chr8g0368271 41.228 114 55 2 54 167 52 153 3.25e-20 88.6
Msa1129920 MtrunA17_Chr5g0434771 33.962 159 97 5 261 417 12 164 1.68e-19 85.9
Msa1129920 MtrunA17_Chr5g0426971 29.897 194 110 6 200 393 46 213 3.47e-18 84.0
Msa1129920 MtrunA17_Chr8g0353961 34.783 138 74 4 30 161 3 130 1.61e-17 79.7
Msa1129920 MtrunA17_Chr5g0443801 33.898 118 75 2 196 313 15 129 2.87e-16 76.6
Msa1129920 MtrunA17_Chr3g0135411 55.738 61 26 1 42 101 52 112 2.29e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1129920 AT5G18460.1 72.030 404 106 4 38 440 33 430 0.0 600
Msa1129920 AT1G55360.1 54.651 430 180 8 11 439 6 421 2.57e-171 487
Msa1129920 AT3G13510.1 54.654 419 173 8 23 439 15 418 1.01e-169 483
Msa1129920 AT5G56530.2 53.721 430 183 7 11 439 5 419 4.99e-167 476
Msa1129920 AT5G56530.1 53.721 430 183 7 11 439 5 419 4.99e-167 476
Msa1129920 AT1G10750.1 58.462 390 138 5 50 439 101 466 3.68e-163 468
Msa1129920 AT1G23340.2 54.374 423 165 9 17 439 14 408 7.08e-163 465
Msa1129920 AT1G23340.1 54.374 423 165 9 17 439 14 408 7.08e-163 465
Msa1129920 AT5G50150.1 55.721 402 154 6 39 439 41 419 1.97e-160 459
Msa1129920 AT1G70550.1 57.179 390 143 8 50 439 99 464 4.26e-158 455
Msa1129920 AT2G44210.1 50.948 422 194 7 19 439 5 414 2.82e-157 451
Msa1129920 AT2G44210.2 50.957 418 168 7 53 439 33 444 6.57e-151 436
Msa1129920 AT2G44220.1 44.681 423 209 7 18 439 4 402 1.08e-133 390
Msa1129920 AT3G48230.1 51.042 384 164 9 58 439 11 372 3.32e-132 386
Msa1129920 AT2G44240.1 43.294 425 211 10 18 439 4 401 1.70e-123 365
Msa1129920 AT2G17750.1 45.063 395 197 9 45 439 21 395 2.31e-123 364
Msa1129920 AT2G19360.1 42.955 440 211 7 19 440 9 426 4.79e-123 364
Msa1129920 AT5G19170.1 43.257 393 191 7 46 434 1 365 2.49e-107 323
Msa1129920 AT2G44250.1 41.121 428 227 10 17 439 1 408 4.36e-107 323
Msa1129920 AT1G23340.3 54.044 272 99 8 44 315 39 284 1.66e-95 289
Msa1129920 AT2G44250.2 42.687 335 176 10 111 439 1 325 9.22e-83 258
Msa1129920 AT5G25950.1 39.000 400 218 10 54 439 44 431 5.35e-81 256
Msa1129920 AT2G20170.1 36.879 423 222 15 22 434 9 396 3.77e-74 238
Msa1129920 AT2G20170.2 36.879 423 222 15 22 434 9 396 3.77e-74 238
Msa1129920 AT5G25960.1 37.949 390 180 10 54 439 25 356 4.21e-70 226
Msa1129920 AT4G23370.1 33.652 419 245 12 25 434 7 401 2.23e-69 226
Msa1129920 AT4G23390.1 36.082 388 206 13 58 434 41 397 1.53e-67 221
Msa1129920 AT2G20170.3 34.434 424 216 15 21 434 8 379 2.05e-64 212
Msa1129920 AT4G23365.1 32.506 403 235 13 41 434 24 398 7.87e-64 211
Msa1129920 AT4G23355.1 32.741 394 232 13 47 434 32 398 2.31e-61 205
Msa1129920 AT1G70550.2 50.230 217 84 8 50 266 99 291 9.54e-61 199
Msa1129920 AT4G23350.1 31.095 402 244 12 41 434 24 400 1.91e-60 202
Msa1129920 AT4G23380.1 32.487 394 222 13 52 434 38 398 3.82e-59 199
Msa1129920 AT4G23360.1 32.506 403 236 14 41 434 24 399 4.68e-59 199
Msa1129920 AT1G10750.2 49.020 204 80 5 50 253 101 280 1.74e-52 180
Msa1129920 AT2G35250.1 34.417 369 182 13 75 436 24 339 1.35e-48 169
Msa1129920 AT4G17505.1 30.933 375 185 12 66 437 18 321 4.07e-45 159
Msa1129920 AT1G10190.1 29.907 428 256 16 19 435 1 395 5.95e-44 158
Msa1129920 AT4G15050.1 30.049 406 242 15 39 435 23 395 6.95e-44 158
Msa1129920 AT2G27320.1 34.579 321 159 10 77 395 30 301 1.34e-42 153
Msa1129920 AT5G46200.1 29.426 401 223 17 77 439 30 408 8.22e-40 147
Msa1129920 AT4G15053.1 30.691 391 232 14 54 435 42 402 1.08e-39 147
Msa1129920 AT4G10220.1 30.233 387 230 15 53 434 47 398 1.02e-38 144
Msa1129920 AT2G24950.1 29.111 450 259 22 6 435 1 410 3.01e-38 143
Msa1129920 AT5G25410.1 27.968 379 224 12 62 434 28 363 3.22e-37 140
Msa1129920 AT5G46810.1 30.108 372 216 15 78 436 11 351 4.96e-37 139
Msa1129920 AT4G23373.1 32.453 265 135 5 25 289 7 227 5.17e-37 137
Msa1129920 AT5G05030.1 29.124 388 218 15 59 436 19 359 5.48e-37 139
Msa1129920 AT5G60380.1 26.984 378 227 13 65 434 31 367 3.43e-36 137
Msa1129920 AT5G46820.1 30.163 368 209 13 78 434 17 347 1.54e-35 135
Msa1129920 AT4G17860.1 28.117 377 205 17 78 439 29 354 4.75e-32 125
Msa1129920 AT4G10210.1 27.358 424 260 16 6 419 1 386 5.46e-30 120
Msa1129920 AT2G38255.1 35.088 228 104 6 78 305 14 197 8.14e-30 115
Msa1129920 AT2G03930.2 56.522 92 40 0 190 281 42 133 3.56e-29 112
Msa1129920 AT4G23080.1 28.894 398 231 17 53 436 47 406 2.00e-28 116
Msa1129920 AT5G11660.1 29.836 305 182 12 139 436 25 304 8.64e-28 112
Msa1129920 AT5G25415.1 29.825 228 141 6 220 436 113 332 1.10e-21 95.9
Msa1129920 AT5G36680.1 27.653 311 186 13 134 434 46 327 3.85e-21 94.4
Msa1129920 AT5G36770.1 27.653 311 186 13 134 434 46 327 3.85e-21 94.4
Msa1129920 AT5G04937.1 29.648 199 113 7 245 436 22 200 3.55e-17 80.1
Msa1129920 AT5G37520.1 32.386 176 102 9 265 434 33 197 5.13e-15 73.9

Find 174 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAAATTTGGATTCTTTC+TGG 0.084366 7_3:-81964029 Msa1129920:CDS
AATTTAGTCTTTCTCAAATT+TGG 0.102248 7_3:-81964040 Msa1129920:CDS
CATTGTTTGGTTTATTTGTT+TGG 0.151929 7_3:-81965863 Msa1129920:five_prime_UTR
TGTCTTAAATATTGTATTAA+TGG 0.166742 7_3:+81965972 None:intergenic
GGTTTATTTGTTTGGGTATA+TGG 0.197181 7_3:-81965855 Msa1129920:five_prime_UTR
GCTAAATCTTTGTATGAATA+TGG 0.199278 7_3:-81964556 Msa1129920:CDS
CTCTAATAATTGTTTGTAAA+AGG 0.210392 7_3:+81965642 None:intergenic
TGGATTCTTTCTGGATCTTT+TGG 0.225879 7_3:-81964020 Msa1129920:CDS
TCGCCGTTATTAACAACTTC+AGG 0.268540 7_3:+81964487 None:intergenic
TGTTTGGGTATATGGTAAAA+TGG 0.269322 7_3:-81965847 Msa1129920:five_prime_UTR
TTGGACAACGTGTCCCATTT+TGG 0.271651 7_3:+81964626 None:intergenic
TGGCAAATGTGGCACCAAAA+TGG 0.279621 7_3:-81964640 Msa1129920:CDS
TGGCGTTGAAAGAGAAATTA+TGG 0.285107 7_3:-81965735 Msa1129920:exon
TTTCTTGAGAGGAAGAAAAT+AGG 0.292445 7_3:+81965909 None:intergenic
GACCCAGATTATTCACTTAC+TGG 0.306757 7_3:-81963499 Msa1129920:CDS
GCGATTGCGTTCACAAAATC+AGG 0.309262 7_3:-81964128 Msa1129920:CDS
TTTGGTGACGGCACATTGAT+AGG 0.315027 7_3:-81963015 Msa1129920:CDS
ATTGTTTGGTTTATTTGTTT+GGG 0.331356 7_3:-81965862 Msa1129920:five_prime_UTR
CACTTTGCTGAAGATGGCTT+TGG 0.334222 7_3:-81962871 Msa1129920:CDS
TTTACCTATTTCTTCTCTCT+TGG 0.342556 7_3:-81965755 Msa1129920:five_prime_UTR
TTGGAAAAGCAAGCTACTTC+AGG 0.347824 7_3:-81962852 Msa1129920:CDS
GCCTATACTTCGTGTAGTTT+AGG 0.363508 7_3:+81965606 None:intergenic
TCCGGCCACTTTGCTGAAGA+TGG 0.363622 7_3:-81962877 Msa1129920:CDS
AAATTTGCGGTTGGCATGGC+TGG 0.366473 7_3:+81966198 None:intergenic
CGTCCAGTAAGTGAATAATC+TGG 0.368717 7_3:+81963496 None:intergenic
CAAAGTAAATCTAACTTTCA+TGG 0.373663 7_3:+81965818 None:intergenic
TATACTACATAAGTACTAAC+TGG 0.375399 7_3:+81962647 None:intergenic
ATAAGAAGAGAGAGGAGAAA+TGG 0.388996 7_3:+81963227 None:intergenic
TGCTACAACCTTCTTTGTGC+TGG 0.397788 7_3:-81963288 Msa1129920:CDS
CCAACACACCTCCACTCAAA+TGG 0.398678 7_3:-81962902 Msa1129920:CDS
TAGGTCAGTCCGGAGCTTTA+TGG 0.400753 7_3:-81963530 Msa1129920:CDS
AGAGCTCCTACAACAACAAA+TGG 0.403956 7_3:-81962741 Msa1129920:CDS
TTGAATGCATAAAACATGAT+TGG 0.414562 7_3:-81962484 Msa1129920:three_prime_UTR
GCATAAAACATGATTGGAAA+TGG 0.416691 7_3:-81962478 Msa1129920:three_prime_UTR
GTATATGATAGTAGTAATAA+TGG 0.420648 7_3:+81966083 None:intergenic
ATAATATTACCTCTATAGTT+AGG 0.422015 7_3:+81965519 None:intergenic
TCGTGCCACCATGGTGCAAT+GGG 0.425828 7_3:-81962956 Msa1129920:CDS
GGCAAATGTGGCACCAAAAT+GGG 0.428900 7_3:-81964639 Msa1129920:CDS
ATAAACCTTCCAGATGAGTA+TGG 0.431310 7_3:+81963182 None:intergenic
CGCCGTTATTAACAACTTCA+GGG 0.431464 7_3:+81964488 None:intergenic
AAATAAACCAAACAATGAAA+AGG 0.432690 7_3:+81965869 None:intergenic
AAATCAGGGGAAGAGGTGTA+TGG 0.433416 7_3:-81964113 Msa1129920:CDS
GGGAACTGGTGGATGAGATT+TGG 0.437839 7_3:-81963033 Msa1129920:CDS
TGTGCCCCATTTGTTGTTGT+AGG 0.439039 7_3:+81962735 None:intergenic
ATATTCATATCCATTCCCTT+TGG 0.441719 7_3:+81964712 None:intergenic
GGCAGAAACAAGAATGGAAA+TGG 0.447953 7_3:+81962531 None:intergenic
GAGCATTGTGGATTGTTGCC+AGG 0.452796 7_3:+81962694 None:intergenic
TCAAGGTTGCACAAACGTTA+AGG 0.453406 7_3:+81966158 None:intergenic
GAGCTCCTACAACAACAAAT+GGG 0.454693 7_3:-81962740 Msa1129920:CDS
AACGCCAAGAGAGAAGAAAT+AGG 0.456202 7_3:+81965751 None:intergenic
GCTGATTGCATTACTAGTAT+TGG 0.461023 7_3:-81965794 Msa1129920:five_prime_UTR
AGGATCCACAACTTGGGAAC+TGG 0.461610 7_3:-81963047 Msa1129920:CDS
ATCGTGCCACCATGGTGCAA+TGG 0.461729 7_3:-81962957 Msa1129920:CDS
AGTGACACATATCAAGCAAC+TGG 0.463842 7_3:-81963312 Msa1129920:CDS
ATTCCCTTTGGCATCGTCGT+TGG 0.464017 7_3:+81964724 None:intergenic
CTAAATCTTTGTATGAATAT+GGG 0.468256 7_3:-81964555 Msa1129920:CDS
AGTGGAGGTGTGTTGGCCAT+TGG 0.481123 7_3:+81962909 None:intergenic
TTTAAAGAATCACAAAATCC+AGG 0.482298 7_3:-81965274 Msa1129920:intron
TTTATGATGCTAGCTGTTCA+AGG 0.482412 7_3:+81966141 None:intergenic
TTGACATCACCATACTCATC+TGG 0.484373 7_3:-81963191 Msa1129920:CDS
ATTGATTAGGATCCACAACT+TGG 0.486292 7_3:-81963054 Msa1129920:intron
TCATTGACTACTTCCACCGT+TGG 0.487439 7_3:+81964062 None:intergenic
CAACACACCTCCACTCAAAT+GGG 0.487631 7_3:-81962901 Msa1129920:CDS
TAGCATTGAAGCTGGTTGGC+AGG 0.488190 7_3:-81963982 Msa1129920:intron
AATGGGACACGTTGTCCAAA+GGG 0.489971 7_3:-81964622 Msa1129920:CDS
AATAAACCAAACAATGAAAA+GGG 0.491298 7_3:+81965870 None:intergenic
GATGAGAGAAGTGGTAAGAG+TGG 0.497824 7_3:-81964670 Msa1129920:CDS
AATCAGGGGAAGAGGTGTAT+GGG 0.502251 7_3:-81964112 Msa1129920:CDS
TGAGGAAAGGATGGTGGGTG+AGG 0.502471 7_3:+81965995 None:intergenic
TTAATAGCATTGAAGCTGGT+TGG 0.503786 7_3:-81963986 Msa1129920:CDS
TGCACCGTGCTCCGCCGTAT+CGG 0.505620 7_3:+81964592 None:intergenic
TTGCACCATGGTGGCACGAT+CGG 0.507100 7_3:+81962960 None:intergenic
ATCCACCAGTTCCCAAGTTG+TGG 0.508328 7_3:+81963042 None:intergenic
CTGGGCTAAGGTTAAGATGT+TGG 0.509928 7_3:+81962786 None:intergenic
GTGGTGAACACTCGCGCCAA+TGG 0.510609 7_3:-81962925 Msa1129920:CDS
AGCAAGAAGAAGATGAGGAG+TGG 0.515521 7_3:+81965679 None:intergenic
TTGCACAAACGTTAAGGAGA+TGG 0.515856 7_3:+81966164 None:intergenic
ACCTCCACTCAAATGGGTTC+CGG 0.515882 7_3:-81962895 Msa1129920:CDS
CGTATCGGTACAGTTCCCTT+TGG 0.516871 7_3:+81964607 None:intergenic
ATTACCTCTATAGTTAGGAC+AGG 0.517511 7_3:+81965524 None:intergenic
CGCGGAGGTGAGACTGTTGT+CGG 0.520352 7_3:+81962810 None:intergenic
CATGGTGCAATGGGGTGGCG+AGG 0.521629 7_3:-81962947 Msa1129920:CDS
AATAATTGTTTGTAAAAGGA+AGG 0.522506 7_3:+81965646 None:intergenic
TCTAGAGGAGATGAGAGAAG+TGG 0.524587 7_3:-81964679 Msa1129920:CDS
AACGACGATGCCAAAGGGAA+TGG 0.525654 7_3:-81964722 Msa1129920:CDS
GCACAAAACCAGCACAAAGA+AGG 0.529315 7_3:+81963280 None:intergenic
TTGTTACTTCAGAGTCAAGA+TGG 0.529370 7_3:-81965351 Msa1129920:intron
ACAAATAGCAAGATAGCCAT+TGG 0.530075 7_3:-81963255 Msa1129920:CDS
TGCACAAACGTTAAGGAGAT+GGG 0.532098 7_3:+81966165 None:intergenic
GCCATCTTCAGCAAAGTGGC+CGG 0.534756 7_3:+81962876 None:intergenic
GAAAGAGAAATAGAAAGAGA+AGG 0.537513 7_3:-81965943 Msa1129920:five_prime_UTR
TTAGCTAAGAGAGACCCTCT+TGG 0.539344 7_3:+81966030 None:intergenic
ATTAATACAATATTTAAGAC+AGG 0.539849 7_3:-81965970 Msa1129920:five_prime_UTR
CTGCTGTCACCATAAAGCTC+CGG 0.539948 7_3:+81963521 None:intergenic
AAGAAAGCAAGAAGAAGATG+AGG 0.541340 7_3:+81965674 None:intergenic
TAAATATTGTATTAATGGTG+AGG 0.541813 7_3:+81965977 None:intergenic
TAATGGTGAGGAAAGGATGG+TGG 0.542775 7_3:+81965989 None:intergenic
TTAGTAGTTTGAGACTTGAA+AGG 0.545306 7_3:-81965580 Msa1129920:CDS
TTGGTGACGGCACATTGATA+GGG 0.548675 7_3:-81963014 Msa1129920:CDS
TTGAGAGGAAGAAAATAGGA+AGG 0.548794 7_3:+81965913 None:intergenic
AGAGAAATTTGCGGTTGGCA+TGG 0.550112 7_3:+81966194 None:intergenic
AGCATTGTGGATTGTTGCCA+GGG 0.551399 7_3:+81962695 None:intergenic
GATCTTAATAGCATTGAAGC+TGG 0.552923 7_3:-81963990 Msa1129920:CDS
TTGCACTAGTTAGGTCAGTC+CGG 0.552965 7_3:-81963540 Msa1129920:intron
ACCTAAACTACACGAAGTAT+AGG 0.555495 7_3:-81965607 Msa1129920:CDS
CAAAGCCATCTTCAGCAAAG+TGG 0.555709 7_3:+81962872 None:intergenic
ACGGCACATTGATAGGGTAC+TGG 0.556765 7_3:-81963008 Msa1129920:CDS
GGTGCAATGGGGTGGCGAGG+TGG 0.560849 7_3:-81962944 Msa1129920:CDS
GTCCAGTAAGTGAATAATCT+GGG 0.564075 7_3:+81963497 None:intergenic
AGGAGAAATGGAAGCTCCAA+TGG 0.564350 7_3:+81963239 None:intergenic
ACAAACAAGAAGGACGGAAG+TGG 0.564408 7_3:+81962510 None:intergenic
AACATGAAGCTAAGATGACT+TGG 0.565165 7_3:+81962596 None:intergenic
TATGGTGATGTCAAATTGCT+TGG 0.565653 7_3:+81963200 None:intergenic
TATTAATGGTGAGGAAAGGA+TGG 0.568483 7_3:+81965986 None:intergenic
CAGATTATTCACTTACTGGA+CGG 0.568692 7_3:-81963495 Msa1129920:intron
CCATTTGAGTGGAGGTGTGT+TGG 0.568898 7_3:+81962902 None:intergenic
AGGCCAACGACGATGCCAAA+GGG 0.574441 7_3:-81964727 Msa1129920:CDS
AAATGGGACACGTTGTCCAA+AGG 0.575071 7_3:-81964623 Msa1129920:CDS
CGATTGCGTTCACAAAATCA+GGG 0.576262 7_3:-81964127 Msa1129920:CDS
GTTCACAAAATCAGGGGAAG+AGG 0.576386 7_3:-81964120 Msa1129920:CDS
AAGGCCAACGACGATGCCAA+AGG 0.577437 7_3:-81964728 Msa1129920:CDS
ATCCACAACTTGGGAACTGG+TGG 0.577654 7_3:-81963044 Msa1129920:CDS
TTGATTAGGATCCACAACTT+GGG 0.578524 7_3:-81963053 Msa1129920:intron
AAAAGCAACAATAAATGTGT+GGG 0.580116 7_3:-81964087 Msa1129920:CDS
AATAAATGTGTGGGATCCAA+CGG 0.581407 7_3:-81964078 Msa1129920:CDS
ATTGTATTAATGGTGAGGAA+AGG 0.583949 7_3:+81965982 None:intergenic
CAAAAGCAACAATAAATGTG+TGG 0.585339 7_3:-81964088 Msa1129920:CDS
AAGTGTGTGAATAACTCCAC+TGG 0.587412 7_3:+81962985 None:intergenic
GGAAGTGGCAGAAACAAGAA+TGG 0.590112 7_3:+81962525 None:intergenic
AATATTAATGAAGAGTCTAG+AGG 0.592197 7_3:-81964694 Msa1129920:CDS
AGAAAAGAGAAATTTGCGGT+TGG 0.593162 7_3:+81966189 None:intergenic
AAATTGGGGAAATTTCAGCT+TGG 0.593582 7_3:+81962424 None:intergenic
TGTCGGTGTCTACAATCTCA+AGG 0.593806 7_3:+81962827 None:intergenic
ACCTCTATAGTTAGGACAGG+GGG 0.594543 7_3:+81965527 None:intergenic
GGGAAGAAAAGAGAAATTTG+CGG 0.594832 7_3:+81966185 None:intergenic
AATGGTGAGGAAAGGATGGT+GGG 0.599351 7_3:+81965990 None:intergenic
CTCGCCACCCCATTGCACCA+TGG 0.599661 7_3:+81962948 None:intergenic
GCCACCATGGTGCAATGGGG+TGG 0.599972 7_3:-81962952 Msa1129920:CDS
CATTCAATCAACAAACAAGA+AGG 0.601184 7_3:+81962500 None:intergenic
CTTAGCCGATCGTGCCACCA+TGG 0.601641 7_3:-81962965 Msa1129920:CDS
TAAGGTTAAGATGTTGGTCG+CGG 0.602338 7_3:+81962792 None:intergenic
CCGTTATTAACAACTTCAGG+GGG 0.604223 7_3:+81964490 None:intergenic
GAAGTTGTTAATAACGGCGA+AGG 0.604884 7_3:-81964484 Msa1129920:CDS
CAAAGGGAACTGTACCGATA+CGG 0.605213 7_3:-81964606 Msa1129920:CDS
TGAGACTTGAAAGGATTCAG+AGG 0.607933 7_3:-81965571 Msa1129920:CDS
GGTTAAGATGTTGGTCGCGG+AGG 0.610177 7_3:+81962795 None:intergenic
TGGACACTATTATTGTTGAG+AGG 0.613110 7_3:-81965715 Msa1129920:CDS
ATTGATAGGGTACTGGCCAG+TGG 0.614292 7_3:-81963001 Msa1129920:CDS
TGTACCGATACGGCGGAGCA+CGG 0.614700 7_3:-81964596 Msa1129920:CDS
TCGTTGCGACGGAATAAAAG+TGG 0.617469 7_3:+81964514 None:intergenic
TTACCTCTATAGTTAGGACA+GGG 0.618276 7_3:+81965525 None:intergenic
GCCGGAACCCATTTGAGTGG+AGG 0.619376 7_3:+81962894 None:intergenic
CATCACCATACTCATCTGGA+AGG 0.620289 7_3:-81963187 Msa1129920:intron
CAATCAACAAACAAGAAGGA+CGG 0.625186 7_3:+81962504 None:intergenic
GTGGTGTGGCATGGCAAATG+TGG 0.628794 7_3:-81964651 Msa1129920:CDS
AGTGATGATCACGAGCATTG+TGG 0.630196 7_3:+81962682 None:intergenic
GTGGCCGGAACCCATTTGAG+TGG 0.633212 7_3:+81962891 None:intergenic
ATCAGGGGAAGAGGTGTATG+GGG 0.633285 7_3:-81964111 Msa1129920:CDS
GCCGTTATTAACAACTTCAG+GGG 0.633552 7_3:+81964489 None:intergenic
GTGGTAAGAGTGGTGTGGCA+TGG 0.656278 7_3:-81964660 Msa1129920:CDS
GCCACCCCATTGCACCATGG+TGG 0.657695 7_3:+81962951 None:intergenic
TCATAACATGATAGTCCAAG+AGG 0.660472 7_3:-81966045 Msa1129920:five_prime_UTR
CATAACATGATAGTCCAAGA+GGG 0.666154 7_3:-81966044 Msa1129920:five_prime_UTR
TTGGCATTATAAGAAGAGAG+AGG 0.666431 7_3:+81963219 None:intergenic
GAGAAGTGGTAAGAGTGGTG+TGG 0.676091 7_3:-81964665 Msa1129920:CDS
AGCTCCTACAACAACAAATG+GGG 0.676238 7_3:-81962739 Msa1129920:CDS
AAATGTGTGGGATCCAACGG+TGG 0.688543 7_3:-81964075 Msa1129920:CDS
TAATAACGGCGAAGGCCATG+AGG 0.692332 7_3:-81964476 Msa1129920:intron
AGGGAACTGTACCGATACGG+CGG 0.708061 7_3:-81964603 Msa1129920:CDS
GATTGCGTTCACAAAATCAG+GGG 0.725289 7_3:-81964126 Msa1129920:CDS
CGTGCCACCATGGTGCAATG+GGG 0.726243 7_3:-81962955 Msa1129920:CDS
TGGTGGATGAGATTTGGTGA+CGG 0.732995 7_3:-81963027 Msa1129920:CDS
TACCTCTATAGTTAGGACAG+GGG 0.733427 7_3:+81965526 None:intergenic
AATATGGGAAGAAACAACGA+CGG 0.747260 7_3:-81964540 Msa1129920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAAATTTTTTAACAGAAA+AGG - chr7_3:81963900-81963919 Msa1129920:intron 10.0%
!!! TTAGACTATAATATTTTTTT+TGG + chr7_3:81964739-81964758 None:intergenic 10.0%
!!! TAATTAGAGATATATTTTAA+AGG + chr7_3:81964831-81964850 None:intergenic 10.0%
!! TGTCTTAAATATTGTATTAA+TGG + chr7_3:81962678-81962697 None:intergenic 15.0%
!!! ATTAATACAATATTTAAGAC+AGG - chr7_3:81962677-81962696 Msa1129920:exon 15.0%
!! AACAATATATATATATAGCT+AGG + chr7_3:81963223-81963242 None:intergenic 15.0%
!! TAATGAACATATAAGAATAA+AGG + chr7_3:81963252-81963271 None:intergenic 15.0%
!!! ATTTTGTGATTCTTTAAAAA+GGG + chr7_3:81963371-81963390 None:intergenic 15.0%
!! AACAGCAAAAAAAAAAAAAA+AGG + chr7_3:81963611-81963630 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTGCTGT+TGG - chr7_3:81963610-81963629 Msa1129920:intron 15.0%
!!! ATTCATTTGTATTTATTTCT+TGG - chr7_3:81963810-81963829 Msa1129920:intron 15.0%
!!! TTTGCTCTAATTTTTTTTTA+AGG - chr7_3:81964257-81964276 Msa1129920:intron 15.0%
!! TTATATTTATAAATGCGATA+TGG + chr7_3:81964359-81964378 None:intergenic 15.0%
!! TCGCATTTATAAATATAAAT+TGG - chr7_3:81964361-81964380 Msa1129920:intron 15.0%
!! ATATTGTTTAAATAATTGAG+AGG - chr7_3:81964444-81964463 Msa1129920:intron 15.0%
!!! CATTTGATTTTTTATTTGTT+TGG - chr7_3:81965214-81965233 Msa1129920:intron 15.0%
!! GTATATGATAGTAGTAATAA+TGG + chr7_3:81962567-81962586 None:intergenic 20.0%
!! TAAATATTGTATTAATGGTG+AGG + chr7_3:81962673-81962692 None:intergenic 20.0%
!! AATAAACCAAACAATGAAAA+GGG + chr7_3:81962780-81962799 None:intergenic 20.0%
!! AAATAAACCAAACAATGAAA+AGG + chr7_3:81962781-81962800 None:intergenic 20.0%
!!! ATTGTTTGGTTTATTTGTTT+GGG - chr7_3:81962785-81962804 Msa1129920:CDS 20.0%
!! AATAATTGTTTGTAAAAGGA+AGG + chr7_3:81963004-81963023 None:intergenic 20.0%
!! CTCTAATAATTGTTTGTAAA+AGG + chr7_3:81963008-81963027 None:intergenic 20.0%
!! ATAATATTACCTCTATAGTT+AGG + chr7_3:81963131-81963150 None:intergenic 20.0%
!!! TTTTGTGATTCTTTAAAAAG+GGG + chr7_3:81963370-81963389 None:intergenic 20.0%
!!! GATTTTGTGATTCTTTAAAA+AGG + chr7_3:81963372-81963391 None:intergenic 20.0%
!! TATATTTCATCACACATATA+TGG - chr7_3:81963398-81963417 Msa1129920:intron 20.0%
!! GGGACAAATTTATAATATTA+AGG + chr7_3:81963493-81963512 None:intergenic 20.0%
!!! TATTTATTTCTTGGTTTGAA+AGG - chr7_3:81963819-81963838 Msa1129920:intron 20.0%
!! CTAAATCTTTGTATGAATAT+GGG - chr7_3:81964092-81964111 Msa1129920:CDS 20.0%
!!! CTAATTTTTTTTTAAGGCAA+TGG - chr7_3:81964263-81964282 Msa1129920:intron 20.0%
!! AATTGAGAGGAAAATAATTA+AGG - chr7_3:81964457-81964476 Msa1129920:intron 20.0%
!!! AATTTAGTCTTTCTCAAATT+TGG - chr7_3:81964607-81964626 Msa1129920:CDS 20.0%
!!! TATAATCTGAAATTGAAGAT+TGG + chr7_3:81964707-81964726 None:intergenic 20.0%
!!! CATATGCAAAAAGAATTTTT+TGG - chr7_3:81964879-81964898 Msa1129920:intron 20.0%
!!! TGATTTTTTATTTGTTTGGT+TGG - chr7_3:81965218-81965237 Msa1129920:intron 20.0%
!!! GATTTTTTATTTGTTTGGTT+GGG - chr7_3:81965219-81965238 Msa1129920:intron 20.0%
!!! TTGTCAATTACTTATTGATT+AGG - chr7_3:81965580-81965599 Msa1129920:CDS 20.0%
!! CATTGTTTGGTTTATTTGTT+TGG - chr7_3:81962784-81962803 Msa1129920:CDS 25.0%
! CAAAGTAAATCTAACTTTCA+TGG + chr7_3:81962832-81962851 None:intergenic 25.0%
! TTTAAAGAATCACAAAATCC+AGG - chr7_3:81963373-81963392 Msa1129920:intron 25.0%
!!! TTTTTTTTGCTGTTGGTTTT+TGG - chr7_3:81963617-81963636 Msa1129920:intron 25.0%
!! ACAAGTTAGTGACATTATTT+TGG + chr7_3:81963703-81963722 None:intergenic 25.0%
! AATATTAATGAAGAGTCTAG+AGG - chr7_3:81963953-81963972 Msa1129920:intron 25.0%
! GCTAAATCTTTGTATGAATA+TGG - chr7_3:81964091-81964110 Msa1129920:CDS 25.0%
! TAATAATAATACACACCTCA+TGG + chr7_3:81964189-81964208 None:intergenic 25.0%
! TAACTACTATCCATTTATGT+TGG - chr7_3:81964315-81964334 Msa1129920:intron 25.0%
! AAAAGCAACAATAAATGTGT+GGG - chr7_3:81964560-81964579 Msa1129920:CDS 25.0%
!!! TTTTGATAATTGCACTAGTT+AGG - chr7_3:81965098-81965117 Msa1129920:intron 25.0%
!! ATTGTAACAGTTTTTGTTCT+GGG + chr7_3:81965882-81965901 None:intergenic 25.0%
!! TATTGTAACAGTTTTTGTTC+TGG + chr7_3:81965883-81965902 None:intergenic 25.0%
! TATACTACATAAGTACTAAC+TGG + chr7_3:81966003-81966022 None:intergenic 25.0%
! TTGAATGCATAAAACATGAT+TGG - chr7_3:81966163-81966182 Msa1129920:five_prime_UTR 25.0%
ATTGTATTAATGGTGAGGAA+AGG + chr7_3:81962668-81962687 None:intergenic 30.0%
GAAAGAGAAATAGAAAGAGA+AGG - chr7_3:81962704-81962723 Msa1129920:CDS 30.0%
TTTCTTGAGAGGAAGAAAAT+AGG + chr7_3:81962741-81962760 None:intergenic 30.0%
! CAATTTCCCTTTTCATTGTT+TGG - chr7_3:81962771-81962790 Msa1129920:CDS 30.0%
GGTTTATTTGTTTGGGTATA+TGG - chr7_3:81962792-81962811 Msa1129920:CDS 30.0%
TGTTTGGGTATATGGTAAAA+TGG - chr7_3:81962800-81962819 Msa1129920:CDS 30.0%
! TTTACCTATTTCTTCTCTCT+TGG - chr7_3:81962892-81962911 Msa1129920:CDS 30.0%
! TTAGTAGTTTGAGACTTGAA+AGG - chr7_3:81963067-81963086 Msa1129920:intron 30.0%
TGTGTGATGAAATATATACC+TGG + chr7_3:81963394-81963413 None:intergenic 30.0%
!! ACTAACTACTCATTTTAGAG+GGG + chr7_3:81963513-81963532 None:intergenic 30.0%
! CACTAACTACTCATTTTAGA+GGG + chr7_3:81963514-81963533 None:intergenic 30.0%
! ACACTAACTACTCATTTTAG+AGG + chr7_3:81963515-81963534 None:intergenic 30.0%
ACTACTTCAACTCATTACAA+AGG + chr7_3:81963646-81963665 None:intergenic 30.0%
ATGTTATATGACGCAGTATA+AGG + chr7_3:81963669-81963688 None:intergenic 30.0%
! GAATAGAAAACGTTTTGCTT+TGG + chr7_3:81963794-81963813 None:intergenic 30.0%
! ATTTCTTGGTTTGAAAGGAA+AGG - chr7_3:81963824-81963843 Msa1129920:intron 30.0%
! TTTCTTGGTTTGAAAGGAAA+GGG - chr7_3:81963825-81963844 Msa1129920:intron 30.0%
AATCCAATTAACTTACTCCT+AGG - chr7_3:81963853-81963872 Msa1129920:intron 30.0%
ATCCAATTAACTTACTCCTA+GGG - chr7_3:81963854-81963873 Msa1129920:intron 30.0%
AACAAAAACTAATCAACCCT+AGG + chr7_3:81963873-81963892 None:intergenic 30.0%
ATATTCATATCCATTCCCTT+TGG + chr7_3:81963938-81963957 None:intergenic 30.0%
! TGGTAGTAATTCTTTTCGTT+TGG - chr7_3:81964381-81964400 Msa1129920:intron 30.0%
CAAAAGCAACAATAAATGTG+TGG - chr7_3:81964559-81964578 Msa1129920:CDS 30.0%
TCTCAAATTTGGATTCTTTC+TGG - chr7_3:81964618-81964637 Msa1129920:CDS 30.0%
AGTAATGTTTCTTCTGAACT+TGG + chr7_3:81964984-81965003 None:intergenic 30.0%
TTCAGAAGAAACATTACTCA+AGG - chr7_3:81964986-81965005 Msa1129920:intron 30.0%
CATTCAATCAACAAACAAGA+AGG + chr7_3:81966150-81966169 None:intergenic 30.0%
GCATAAAACATGATTGGAAA+TGG - chr7_3:81966169-81966188 Msa1129920:five_prime_UTR 30.0%
AGAAAAGAGAAATTTGCGGT+TGG + chr7_3:81962461-81962480 None:intergenic 35.0%
GGGAAGAAAAGAGAAATTTG+CGG + chr7_3:81962465-81962484 None:intergenic 35.0%
TTTATGATGCTAGCTGTTCA+AGG + chr7_3:81962509-81962528 None:intergenic 35.0%
TCATAACATGATAGTCCAAG+AGG - chr7_3:81962602-81962621 Msa1129920:three_prime_UTR 35.0%
CATAACATGATAGTCCAAGA+GGG - chr7_3:81962603-81962622 Msa1129920:three_prime_UTR 35.0%
TATTAATGGTGAGGAAAGGA+TGG + chr7_3:81962664-81962683 None:intergenic 35.0%
TTGAGAGGAAGAAAATAGGA+AGG + chr7_3:81962737-81962756 None:intergenic 35.0%
! GAAAAACGACTTTTCTTGAG+AGG + chr7_3:81962752-81962771 None:intergenic 35.0%
GCTGATTGCATTACTAGTAT+TGG - chr7_3:81962853-81962872 Msa1129920:CDS 35.0%
! TGGCGTTGAAAGAGAAATTA+TGG - chr7_3:81962912-81962931 Msa1129920:CDS 35.0%
TGGACACTATTATTGTTGAG+AGG - chr7_3:81962932-81962951 Msa1129920:CDS 35.0%
AAGAAAGCAAGAAGAAGATG+AGG + chr7_3:81962976-81962995 None:intergenic 35.0%
ACCTAAACTACACGAAGTAT+AGG - chr7_3:81963040-81963059 Msa1129920:CDS 35.0%
TTACCTCTATAGTTAGGACA+GGG + chr7_3:81963125-81963144 None:intergenic 35.0%
ATTACCTCTATAGTTAGGAC+AGG + chr7_3:81963126-81963145 None:intergenic 35.0%
! TTGTTACTTCAGAGTCAAGA+TGG - chr7_3:81963296-81963315 Msa1129920:CDS 35.0%
! CTTTAAAAAGGGGTGATCTA+AGG + chr7_3:81963360-81963379 None:intergenic 35.0%
CATGAAACGCTAGTACAAAA+TGG + chr7_3:81963465-81963484 None:intergenic 35.0%
! CATTTTGTACTAGCGTTTCA+TGG - chr7_3:81963463-81963482 Msa1129920:intron 35.0%
AACCCTAGGAGTAAGTTAAT+TGG + chr7_3:81963859-81963878 None:intergenic 35.0%
AATATGGGAAGAAACAACGA+CGG - chr7_3:81964107-81964126 Msa1129920:CDS 35.0%
! AAGGCAATGGTCTCTATTTT+TGG - chr7_3:81964276-81964295 Msa1129920:intron 35.0%
AAATGCGATATGGACAGTTA+GGG + chr7_3:81964349-81964368 None:intergenic 35.0%
TAAATGCGATATGGACAGTT+AGG + chr7_3:81964350-81964369 None:intergenic 35.0%
!! AAGGAACTAATTTGCTTGAG+TGG - chr7_3:81964476-81964495 Msa1129920:intron 35.0%
AATAAATGTGTGGGATCCAA+CGG - chr7_3:81964569-81964588 Msa1129920:CDS 35.0%
! TGGATTCTTTCTGGATCTTT+TGG - chr7_3:81964627-81964646 Msa1129920:CDS 35.0%
! ATTCTTTCTGGATCTTTTGG+TGG - chr7_3:81964630-81964649 Msa1129920:CDS 35.0%
! GATCTTAATAGCATTGAAGC+TGG - chr7_3:81964657-81964676 Msa1129920:CDS 35.0%
! TTAATAGCATTGAAGCTGGT+TGG - chr7_3:81964661-81964680 Msa1129920:CDS 35.0%
GTCCAGTAAGTGAATAATCT+GGG + chr7_3:81965153-81965172 None:intergenic 35.0%
CAGATTATTCACTTACTGGA+CGG - chr7_3:81965152-81965171 Msa1129920:intron 35.0%
ACAAATAGCAAGATAGCCAT+TGG - chr7_3:81965392-81965411 Msa1129920:intron 35.0%
ATAAGAAGAGAGAGGAGAAA+TGG + chr7_3:81965423-81965442 None:intergenic 35.0%
TTGGCATTATAAGAAGAGAG+AGG + chr7_3:81965431-81965450 None:intergenic 35.0%
! TATGGTGATGTCAAATTGCT+TGG + chr7_3:81965450-81965469 None:intergenic 35.0%
ATAAACCTTCCAGATGAGTA+TGG + chr7_3:81965468-81965487 None:intergenic 35.0%
ATTGATTAGGATCCACAACT+TGG - chr7_3:81965593-81965612 Msa1129920:CDS 35.0%
TTGATTAGGATCCACAACTT+GGG - chr7_3:81965594-81965613 Msa1129920:CDS 35.0%
AACATGAAGCTAAGATGACT+TGG + chr7_3:81966054-81966073 None:intergenic 35.0%
CAATCAACAAACAAGAAGGA+CGG + chr7_3:81966146-81966165 None:intergenic 35.0%
TGCACAAACGTTAAGGAGAT+GGG + chr7_3:81962485-81962504 None:intergenic 40.0%
TTGCACAAACGTTAAGGAGA+TGG + chr7_3:81962486-81962505 None:intergenic 40.0%
TCAAGGTTGCACAAACGTTA+AGG + chr7_3:81962492-81962511 None:intergenic 40.0%
AACGCCAAGAGAGAAGAAAT+AGG + chr7_3:81962899-81962918 None:intergenic 40.0%
GCCTATACTTCGTGTAGTTT+AGG + chr7_3:81963044-81963063 None:intergenic 40.0%
TGAGACTTGAAAGGATTCAG+AGG - chr7_3:81963076-81963095 Msa1129920:intron 40.0%
TACCTCTATAGTTAGGACAG+GGG + chr7_3:81963124-81963143 None:intergenic 40.0%
CCGTTATTAACAACTTCAGG+GGG + chr7_3:81964160-81964179 None:intergenic 40.0%
CCCCCTGAAGTTGTTAATAA+CGG - chr7_3:81964157-81964176 Msa1129920:intron 40.0%
GCCGTTATTAACAACTTCAG+GGG + chr7_3:81964161-81964180 None:intergenic 40.0%
CGCCGTTATTAACAACTTCA+GGG + chr7_3:81964162-81964181 None:intergenic 40.0%
TCGCCGTTATTAACAACTTC+AGG + chr7_3:81964163-81964182 None:intergenic 40.0%
GAAGTTGTTAATAACGGCGA+AGG - chr7_3:81964163-81964182 Msa1129920:intron 40.0%
GGACATAGGACCAACATAAA+TGG + chr7_3:81964328-81964347 None:intergenic 40.0%
ATATGGACAGTTAGGGACAT+AGG + chr7_3:81964342-81964361 None:intergenic 40.0%
CGATTGCGTTCACAAAATCA+GGG - chr7_3:81964520-81964539 Msa1129920:CDS 40.0%
GATTGCGTTCACAAAATCAG+GGG - chr7_3:81964521-81964540 Msa1129920:CDS 40.0%
GACCCAGATTATTCACTTAC+TGG - chr7_3:81965148-81965167 Msa1129920:intron 40.0%
CGTCCAGTAAGTGAATAATC+TGG + chr7_3:81965154-81965173 None:intergenic 40.0%
AGTGACACATATCAAGCAAC+TGG - chr7_3:81965335-81965354 Msa1129920:CDS 40.0%
TTGACATCACCATACTCATC+TGG - chr7_3:81965456-81965475 Msa1129920:intron 40.0%
AAGTGTGTGAATAACTCCAC+TGG + chr7_3:81965665-81965684 None:intergenic 40.0%
TTGGAAAAGCAAGCTACTTC+AGG - chr7_3:81965795-81965814 Msa1129920:five_prime_UTR 40.0%
TAAGGTTAAGATGTTGGTCG+CGG + chr7_3:81965858-81965877 None:intergenic 40.0%
!! ACAGTTTTTGTTCTGGGCTA+AGG + chr7_3:81965876-81965895 None:intergenic 40.0%
AGAGCTCCTACAACAACAAA+TGG - chr7_3:81965906-81965925 Msa1129920:five_prime_UTR 40.0%
GAGCTCCTACAACAACAAAT+GGG - chr7_3:81965907-81965926 Msa1129920:five_prime_UTR 40.0%
AGCTCCTACAACAACAAATG+GGG - chr7_3:81965908-81965927 Msa1129920:five_prime_UTR 40.0%
! TGGGGCACATACTTTTACTA+TGG - chr7_3:81965926-81965945 Msa1129920:five_prime_UTR 40.0%
! GGCACATACTTTTACTATGG+TGG - chr7_3:81965929-81965948 Msa1129920:five_prime_UTR 40.0%
GGCAGAAACAAGAATGGAAA+TGG + chr7_3:81966119-81966138 None:intergenic 40.0%
AGAGAAATTTGCGGTTGGCA+TGG + chr7_3:81962456-81962475 None:intergenic 45.0%
TTAGCTAAGAGAGACCCTCT+TGG + chr7_3:81962620-81962639 None:intergenic 45.0%
AATGGTGAGGAAAGGATGGT+GGG + chr7_3:81962660-81962679 None:intergenic 45.0%
TAATGGTGAGGAAAGGATGG+TGG + chr7_3:81962661-81962680 None:intergenic 45.0%
AGCAAGAAGAAGATGAGGAG+TGG + chr7_3:81962971-81962990 None:intergenic 45.0%
ACCTCTATAGTTAGGACAGG+GGG + chr7_3:81963123-81963142 None:intergenic 45.0%
! AAAAAGGGGTGATCTAAGGC+TGG + chr7_3:81963356-81963375 None:intergenic 45.0%
TCTAGAGGAGATGAGAGAAG+TGG - chr7_3:81963968-81963987 Msa1129920:intron 45.0%
GATGAGAGAAGTGGTAAGAG+TGG - chr7_3:81963977-81963996 Msa1129920:intron 45.0%
TGGCAAATGTGGCACCAAAA+TGG - chr7_3:81964007-81964026 Msa1129920:CDS 45.0%
GGCAAATGTGGCACCAAAAT+GGG - chr7_3:81964008-81964027 Msa1129920:CDS 45.0%
! TTGGACAACGTGTCCCATTT+TGG + chr7_3:81964024-81964043 None:intergenic 45.0%
AAATGGGACACGTTGTCCAA+AGG - chr7_3:81964024-81964043 Msa1129920:CDS 45.0%
AATGGGACACGTTGTCCAAA+GGG - chr7_3:81964025-81964044 Msa1129920:CDS 45.0%
! CAAAGGGAACTGTACCGATA+CGG - chr7_3:81964041-81964060 Msa1129920:CDS 45.0%
TCGTTGCGACGGAATAAAAG+TGG + chr7_3:81964136-81964155 None:intergenic 45.0%
GCGATTGCGTTCACAAAATC+AGG - chr7_3:81964519-81964538 Msa1129920:CDS 45.0%
GTTCACAAAATCAGGGGAAG+AGG - chr7_3:81964527-81964546 Msa1129920:CDS 45.0%
! AAATCAGGGGAAGAGGTGTA+TGG - chr7_3:81964534-81964553 Msa1129920:CDS 45.0%
! AATCAGGGGAAGAGGTGTAT+GGG - chr7_3:81964535-81964554 Msa1129920:CDS 45.0%
! TCATTGACTACTTCCACCGT+TGG + chr7_3:81964588-81964607 None:intergenic 45.0%
TTGCACTAGTTAGGTCAGTC+CGG - chr7_3:81965107-81965126 Msa1129920:intron 45.0%
TGCTACAACCTTCTTTGTGC+TGG - chr7_3:81965359-81965378 Msa1129920:intron 45.0%
GCACAAAACCAGCACAAAGA+AGG + chr7_3:81965370-81965389 None:intergenic 45.0%
AGGAGAAATGGAAGCTCCAA+TGG + chr7_3:81965411-81965430 None:intergenic 45.0%
CATCACCATACTCATCTGGA+AGG - chr7_3:81965460-81965479 Msa1129920:intron 45.0%
!! TGGTGGATGAGATTTGGTGA+CGG - chr7_3:81965620-81965639 Msa1129920:CDS 45.0%
!! TTTGGTGACGGCACATTGAT+AGG - chr7_3:81965632-81965651 Msa1129920:CDS 45.0%
!! TTGGTGACGGCACATTGATA+GGG - chr7_3:81965633-81965652 Msa1129920:CDS 45.0%
CAACACACCTCCACTCAAAT+GGG - chr7_3:81965746-81965765 Msa1129920:five_prime_UTR 45.0%
CAAAGCCATCTTCAGCAAAG+TGG + chr7_3:81965778-81965797 None:intergenic 45.0%
CACTTTGCTGAAGATGGCTT+TGG - chr7_3:81965776-81965795 Msa1129920:five_prime_UTR 45.0%
! TGTCGGTGTCTACAATCTCA+AGG + chr7_3:81965823-81965842 None:intergenic 45.0%
CTGGGCTAAGGTTAAGATGT+TGG + chr7_3:81965864-81965883 None:intergenic 45.0%
!! TGTGCCCCATTTGTTGTTGT+AGG + chr7_3:81965915-81965934 None:intergenic 45.0%
! TACTTTTACTATGGTGGCCC+TGG - chr7_3:81965935-81965954 Msa1129920:five_prime_UTR 45.0%
! AGCATTGTGGATTGTTGCCA+GGG + chr7_3:81965955-81965974 None:intergenic 45.0%
! AGTGATGATCACGAGCATTG+TGG + chr7_3:81965968-81965987 None:intergenic 45.0%
GGAAGTGGCAGAAACAAGAA+TGG + chr7_3:81966125-81966144 None:intergenic 45.0%
ACAAACAAGAAGGACGGAAG+TGG + chr7_3:81966140-81966159 None:intergenic 45.0%
!!! TAATTTTTATAAGAATTAAT+TGG + chr7_3:81963156-81963175 None:intergenic 5.0%
AAATTTGCGGTTGGCATGGC+TGG + chr7_3:81962452-81962471 None:intergenic 50.0%
ATTCCCTTTGGCATCGTCGT+TGG + chr7_3:81963926-81963945 None:intergenic 50.0%
AACGACGATGCCAAAGGGAA+TGG - chr7_3:81963925-81963944 Msa1129920:intron 50.0%
GAGAAGTGGTAAGAGTGGTG+TGG - chr7_3:81963982-81964001 Msa1129920:intron 50.0%
! CGTATCGGTACAGTTCCCTT+TGG + chr7_3:81964043-81964062 None:intergenic 50.0%
TAATAACGGCGAAGGCCATG+AGG - chr7_3:81964171-81964190 Msa1129920:intron 50.0%
! ATCAGGGGAAGAGGTGTATG+GGG - chr7_3:81964536-81964555 Msa1129920:CDS 50.0%
AAATGTGTGGGATCCAACGG+TGG - chr7_3:81964572-81964591 Msa1129920:CDS 50.0%
! TAGCATTGAAGCTGGTTGGC+AGG - chr7_3:81964665-81964684 Msa1129920:CDS 50.0%
TAGGTCAGTCCGGAGCTTTA+TGG - chr7_3:81965117-81965136 Msa1129920:intron 50.0%
CTGCTGTCACCATAAAGCTC+CGG + chr7_3:81965129-81965148 None:intergenic 50.0%
AGGATCCACAACTTGGGAAC+TGG - chr7_3:81965600-81965619 Msa1129920:CDS 50.0%
ATCCACAACTTGGGAACTGG+TGG - chr7_3:81965603-81965622 Msa1129920:CDS 50.0%
ATCCACCAGTTCCCAAGTTG+TGG + chr7_3:81965608-81965627 None:intergenic 50.0%
! GGGAACTGGTGGATGAGATT+TGG - chr7_3:81965614-81965633 Msa1129920:CDS 50.0%
ACGGCACATTGATAGGGTAC+TGG - chr7_3:81965639-81965658 Msa1129920:CDS 50.0%
!! ATTGATAGGGTACTGGCCAG+TGG - chr7_3:81965646-81965665 Msa1129920:CDS 50.0%
! CCATTTGAGTGGAGGTGTGT+TGG + chr7_3:81965748-81965767 None:intergenic 50.0%
CCAACACACCTCCACTCAAA+TGG - chr7_3:81965745-81965764 Msa1129920:five_prime_UTR 50.0%
! ACCTCCACTCAAATGGGTTC+CGG - chr7_3:81965752-81965771 Msa1129920:five_prime_UTR 50.0%
! GAGCATTGTGGATTGTTGCC+AGG + chr7_3:81965956-81965975 None:intergenic 50.0%
!! TGAGGAAAGGATGGTGGGTG+AGG + chr7_3:81962655-81962674 None:intergenic 55.0%
GCCCCCTGTCCTAACTATAG+AGG - chr7_3:81963119-81963138 Msa1129920:intron 55.0%
AAGGCCAACGACGATGCCAA+AGG - chr7_3:81963919-81963938 Msa1129920:intron 55.0%
AGGCCAACGACGATGCCAAA+GGG - chr7_3:81963920-81963939 Msa1129920:intron 55.0%
GTGGTAAGAGTGGTGTGGCA+TGG - chr7_3:81963987-81964006 Msa1129920:CDS 55.0%
GTGGTGTGGCATGGCAAATG+TGG - chr7_3:81963996-81964015 Msa1129920:CDS 55.0%
! AGGGAACTGTACCGATACGG+CGG - chr7_3:81964044-81964063 Msa1129920:CDS 55.0%
TTGCACCATGGTGGCACGAT+CGG + chr7_3:81965690-81965709 None:intergenic 55.0%
ATCGTGCCACCATGGTGCAA+TGG - chr7_3:81965690-81965709 Msa1129920:CDS 55.0%
TCGTGCCACCATGGTGCAAT+GGG - chr7_3:81965691-81965710 Msa1129920:CDS 55.0%
! AGTGGAGGTGTGTTGGCCAT+TGG + chr7_3:81965741-81965760 None:intergenic 55.0%
! TCCGGCCACTTTGCTGAAGA+TGG - chr7_3:81965770-81965789 Msa1129920:five_prime_UTR 55.0%
GCCATCTTCAGCAAAGTGGC+CGG + chr7_3:81965774-81965793 None:intergenic 55.0%
GGTTAAGATGTTGGTCGCGG+AGG + chr7_3:81965855-81965874 None:intergenic 55.0%
! TGTACCGATACGGCGGAGCA+CGG - chr7_3:81964051-81964070 Msa1129920:CDS 60.0%
CTTAGCCGATCGTGCCACCA+TGG - chr7_3:81965682-81965701 Msa1129920:CDS 60.0%
CGTGCCACCATGGTGCAATG+GGG - chr7_3:81965692-81965711 Msa1129920:CDS 60.0%
GTGGTGAACACTCGCGCCAA+TGG - chr7_3:81965722-81965741 Msa1129920:exon 60.0%
GCCGGAACCCATTTGAGTGG+AGG + chr7_3:81965756-81965775 None:intergenic 60.0%
GTGGCCGGAACCCATTTGAG+TGG + chr7_3:81965759-81965778 None:intergenic 60.0%
CGCGGAGGTGAGACTGTTGT+CGG + chr7_3:81965840-81965859 None:intergenic 60.0%
TGCACCGTGCTCCGCCGTAT+CGG + chr7_3:81964058-81964077 None:intergenic 65.0%
CTTCAGGGGGCTCGTTGCGA+CGG + chr7_3:81964147-81964166 None:intergenic 65.0%
GCCACCATGGTGCAATGGGG+TGG - chr7_3:81965695-81965714 Msa1129920:CDS 65.0%
GCCACCCCATTGCACCATGG+TGG + chr7_3:81965699-81965718 None:intergenic 65.0%
CTCGCCACCCCATTGCACCA+TGG + chr7_3:81965702-81965721 None:intergenic 65.0%
CATGGTGCAATGGGGTGGCG+AGG - chr7_3:81965700-81965719 Msa1129920:CDS 65.0%
GGTGCAATGGGGTGGCGAGG+TGG - chr7_3:81965703-81965722 Msa1129920:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 81962431 81966238 81962431 ID=Msa1129920;Name=Msa1129920
chr7_3 mRNA 81962431 81966238 81962431 ID=Msa1129920-mRNA-1;Parent=Msa1129920;Name=Msa1129920-mRNA-1;_AED=0.07;_eAED=0.07;_QI=500|0.83|0.85|1|1|1|7|259|440
chr7_3 exon 81962431 81963067 81962431 ID=Msa1129920-mRNA-1:exon:24012;Parent=Msa1129920-mRNA-1
chr7_3 exon 81963188 81963334 81963188 ID=Msa1129920-mRNA-1:exon:24011;Parent=Msa1129920-mRNA-1
chr7_3 exon 81963496 81963549 81963496 ID=Msa1129920-mRNA-1:exon:24010;Parent=Msa1129920-mRNA-1
chr7_3 exon 81963983 81964153 81963983 ID=Msa1129920-mRNA-1:exon:24009;Parent=Msa1129920-mRNA-1
chr7_3 exon 81964477 81964752 81964477 ID=Msa1129920-mRNA-1:exon:24008;Parent=Msa1129920-mRNA-1
chr7_3 exon 81965275 81965361 81965275 ID=Msa1129920-mRNA-1:exon:24007;Parent=Msa1129920-mRNA-1
chr7_3 exon 81965529 81966238 81965529 ID=Msa1129920-mRNA-1:exon:24006;Parent=Msa1129920-mRNA-1
chr7_3 five_prime_UTR 81965739 81966238 81965739 ID=Msa1129920-mRNA-1:five_prime_utr;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81965529 81965738 81965529 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81965275 81965361 81965275 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81964477 81964752 81964477 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81963983 81964153 81963983 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81963496 81963549 81963496 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81963188 81963334 81963188 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 CDS 81962690 81963067 81962690 ID=Msa1129920-mRNA-1:cds;Parent=Msa1129920-mRNA-1
chr7_3 three_prime_UTR 81962431 81962689 81962431 ID=Msa1129920-mRNA-1:three_prime_utr;Parent=Msa1129920-mRNA-1
Gene Sequence

>Msa1129920

ATGGACACTATTATTGTTGAGAGGAAGAAAAGAATTTCCACTCCTCATCTTCTTCTTGCTTTCTTGTTCACCTTCCTTTTACAAACAATTATTAGAGTTGAATGCATCAACCTAAACTACACGAAGTATAGGCAAGTTAGTAGTTTGAGACTTGAAAGGATTCAGAGGCACTTGAACAAGATTAACAAGCCCCCTGTCCTAACTATAGAGAGTCAAGATGGTGATCTCATAGATTGTGTTCACAAAAGAAAGCAACCAGCCTTAGATCACCCCTTTTTAAAGAATCACAAAATCCAGAAAAGGCCAACGACGATGCCAAAGGGAATGGATATGAATATTAATGAAGAGTCTAGAGGAGATGAGAGAAGTGGTAAGAGTGGTGTGGCATGGCAAATGTGGCACCAAAATGGGACACGTTGTCCAAAGGGAACTGTACCGATACGGCGGAGCACGGTGCATGATGTGTTGAGAGCTAAATCTTTGTATGAATATGGGAAGAAACAACGACGGTCACCACTTTTATTCCGTCGCAACGAGCCCCCTGAAGTTGTTAATAACGGCGAAGGCCATGAGCATGCGATTGCGTTCACAAAATCAGGGGAAGAGGTGTATGGGGCAAAAGCAACAATAAATGTGTGGGATCCAACGGTGGAAGTAGTCAATGAATTTAGTCTTTCTCAAATTTGGATTCTTTCTGGATCTTTTGGTGGCTCTGATCTTAATAGCATTGAAGCTGGTTGGCAGGTCAGTCCGGAGCTTTATGGTGACAGCAGACCCAGATTATTCACTTACTGGACGAGTGACACATATCAAGCAACTGGATGCTACAACCTTCTTTGTGCTGGTTTTGTGCAAACAAATAGCAAGATAGCCATTGGAGCTTCCATTTCTCCTCTCTCTTCTTATAATGCCAAGCAATTTGACATCACCATACTCATCTGGAAGGATCCACAACTTGGGAACTGGTGGATGAGATTTGGTGACGGCACATTGATAGGGTACTGGCCAGTGGAGTTATTCACACACTTAGCCGATCGTGCCACCATGGTGCAATGGGGTGGCGAGGTGGTGAACACTCGCGCCAATGGCCAACACACCTCCACTCAAATGGGTTCCGGCCACTTTGCTGAAGATGGCTTTGGAAAAGCAAGCTACTTCAGGAACCTTGAGATTGTAGACACCGACAACAGTCTCACCTCCGCGACCAACATCTTAACCTTAGCCCAGAACAAAAACTGTTACAATATCAAGAGCTCCTACAACAACAAATGGGGCACATACTTTTACTATGGTGGCCCTGGCAACAATCCACAATGCTCGTGA
Protein sequence

>Msa1129920

MDTIIVERKKRISTPHLLLAFLFTFLLQTIIRVECINLNYTKYRQVSSLRLERIQRHLNKINKPPVLTIESQDGDLIDCVHKRKQPALDHPFLKNHKIQKRPTTMPKGMDMNINEESRGDERSGKSGVAWQMWHQNGTRCPKGTVPIRRSTVHDVLRAKSLYEYGKKQRRSPLLFRRNEPPEVVNNGEGHEHAIAFTKSGEEVYGAKATINVWDPTVEVVNEFSLSQIWILSGSFGGSDLNSIEAGWQVSPELYGDSRPRLFTYWTSDTYQATGCYNLLCAGFVQTNSKIAIGASISPLSSYNAKQFDITILIWKDPQLGNWWMRFGDGTLIGYWPVELFTHLADRATMVQWGGEVVNTRANGQHTSTQMGSGHFAEDGFGKASYFRNLEIVDTDNSLTSATNILTLAQNKNCYNIKSSYNNKWGTYFYYGGPGNNPQCS*